Digital Commons Network^™

A Turn-Off Fluorescent Sensor For Metal Ions Quantifies Corrosion In An Organic Solvent, Lianlian Liu, Zechariah Pfaffenberger, Mark Siegel, Anuj Saini, Lydia Kisley

Faculty Scholarship

We demonstrate that the corrosion of AISI 1045 medium carbon steel and pure aluminum can be quantified by the turn-off fluorescent sensor Phen Green-SK (PGSK) in ethanol-based solutions. We first evaluate the dependence of the chelation enhanced quenching of PGSK on iron and aluminum ion concentrations. Subsequently, we apply PGSK to examine the anodic dissolution of metal corrosion. The observed time-dependent PGSK-quenching quantifies the corrosion rates of two metals over 24 h of immersion in ethanol-based solutions. The PGSK-based quantification of corrosion is compared to scanning electron microscopy and electrochemical techniques, including open circuit potential and Tafel extrapolation. The corrosion …

High-Density Genetic Map Construction And Qtl Mapping Of A Zigzag-Shaped Stem Trait In Tea Plant (Camellia Sinensis), Dingding Liu, Yuanyuan Ye, Rongjin Tang, Yang Gong, Si Chen, Chenyu Zhang, Piao Mei, Jiedan Chen, Liang Chen, Chunlei Ma

Faculty, Staff and Student Publications

The highly unique zigzag-shaped stem phenotype in tea plants boasts significant ornamental value and is exceptionally rare. To investigate the genetic mechanism behind this trait, we developed BC1 artificial hybrid populations. Our genetic analysis revealed the zigzag-shaped trait as a qualitative trait. Utilizing whole-genome resequencing, we constructed a high-density genetic map from the BC1 population, incorporating 5,250 SNP markers across 15 linkage groups, covering 3,328.51 cM with an average marker interval distance of 0.68 cM. A quantitative trait locus (QTL) for the zigzag-shaped trait was identified on chromosome 4, within a 61.2 to 97.2 Mb range, accounting for a phenotypic …

Can Chatgpt Keep A Secret? An Evaluation Of The Applicability And Suitability Of Trade Secrecy Protection For Ai-Generated Inventions, Gina Campanelli

Duke Law & Technology Review

The rising popularity of generative artificial intelligence has sparked questions around whether AI-generated inventions and works can be protected under current intellectual property regimes, and if so, how. Guidance from the U.S. Copyright Office and recent court cases shed some light on the applicability of copyright and patent protection to AI-generated products; namely “authors” and “inventors” are limited to natural persons. But further developments in copyright and patent law are still lagging behind generative-AI’s rapid growth. Trade secrecy emerges as the most viable path forward to protect AI-generated works and inventions because ownership of trade secrets is not limited to …

Draft Final 2024 Butte Priority Soils Operable Unit (Bpsou) Borrow Amendment Submittal #1, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Clinical Significance Of Pno1 As A Novel Biomarker And Therapeutic Target Of Hepatocellular Carcinoma, Sanjit K. Roy, Shivam Srivastava, Caroline Mccance, Anju Shrivastava, Jason Morvant, Sharmila Shankar, Rakesh K. Srivastava

School of Medicine Faculty Publications

The RNA-binding protein PNO1 plays an essential role in ribosome biogenesis. Recent studies have shown that it is involved in tumorigenesis; however, its role in hepatocellular carcinoma (HCC) is not well understood. The purpose of this study was to examine whether PNO1 can be used as a biomarker of HCC and also examine the therapeutic potential of PNO1 knockout for the treatment of HCC. PNO1 expression was upregulated in HCC and associated with poor prognosis. PNO1 expression was positively associated with tumour stage, lymph node metastasis and poor survival. PNO1 expression was significantly higher in HCC compared to that in …

Activation Of Interleukin 33-Nfκb Axis In Granulosa Cells During Atresia And Its Role In Disposal Of Atretic Follicles, Jean Wu, Colin Carlock, Kiana Tatum, Junbo Shim, Cindy Zhou, Yahuan Lou

Faculty, Staff and Student Publications

It has been previously shown that the cytokine interleukin 33 is required for two processes, i.e., autophagic digestion of granulosa cells and recruitment of macrophages into atretic follicles, for full disposal of atretic follicles. Now, this study shows that activation of interleukin 33-suppression of tumorigenicity 2-Nuclear Factor ĸB (NFκB) axis in granulosa in early atretic follicles may regulate those two events. Injection of human chorionic gonadotropin has been shown to induce a transient peak of interleukin 33 expression with synchronized atresia. In this model, interleukin 33-independent expression of suppression of tumorigenicity 2 in granulosa cells was detected in early atretic …

Exploring The Intersection Of Mental Health, Self-Advocacy, And Sense Of Belonging Among Rural, First-Generation College Students, Sarah Johnson

Dissertations, 2020-current

This dissertation delves into the intersections of social and cultural capital, self-advocacy, sense of belonging, and the experiences of mental health help-seeking among rural, first-generation college students. Rural, first-generation college students face unique challenges throughout their academic journey, this dissertation aims to contribute to the literature by examining the experiences and support mechanisms that are crucial for this population to be successful in higher education. This dissertation follows a triple-manuscript style, including a conceptual manuscript, quantitative manuscript, and a qualitative manuscript. The conceptual manuscript examined how college counselors can build contextual social and cultural capital among rural, first-generation college students …

Using Recurrent Neural Networks To Predict Mastery Outcomes From Learning Journey Clickstream Data, Sean A. York

Dissertations, 2020-current

In this study, I use recurrent neural networks (RNN) and modern natural language processing techniques to analyze clickstream activity data from an automated, online, mastery learning platform called the Indiana University Plagiarism Tutorials and Tests (IPTAT). IPTAT offers learning materials and certification tests on recognizing and avoiding plagiarism, and has awarded over 1.17 million certificates since 2016. Learners may use or not use resources as they wish, but must pass a randomized test drawn from a large item pool to obtain a certificate. Most learners pass after one to seven test attempts, but some take as many as 50 randomized …

Does Breaking Up Moderate Intensity Continuous Exercise Into Accumulated Bouts Improve Postprandial Metabolic Outcomes In Middle Aged And Older Adults?, Miranda Janis

Masters Theses, 2020-current

Due to the high incidence of CVD in older adults, low levels of habitual physical activity, and poor dietary habits, it is imperative to determine an effective minimal dose of exercise that is attainable to mitigate adverse postprandial (post-meal) responses. This study is the first to our knowledge to assess whether accumulated exercise to expend 25% of the calories consumed in a high-fat meal (HFM) of moderate intensity walking will lower postprandial triglycerides (TRG), glucose (GLU) and metabolic load index (MLI: summation of GLU and TRG), compared to continuous exercise, in middle-aged and older adults (OA). Methods: Ten OA (n=10, …

Characterizing The Expression Of The Sphingosine 1-Phosphate Pathway In Injury And Post-Traumatic Fibrosis, Nicole Cubbage

Masters Theses, 2020-current

Following injury, tissue repair allows for the restoration of the architecture and function of the injured tissue, while tissue replacement is characterized by severely damaged or non-regenerable tissue that is repaired by the laying down of extensive connective tissue, also known as fibrosis. The fibrotic process should be arrested at its early stage to have the greatest impact on preventing or reversing associate sequelae and promoting the regeneration of normal tissue. Sphingosine 1-phosphate (S1P), a metabolic product of cell membrane sphingolipids, has emerged as a pathway that may be involved in fibrosis. Most of S1P’s actions are mediated through the …

The Relationship Among Oral Contraceptive Use, Sleep, And Sexual Behavior, Sureyya Sanchez

Masters Theses, 2020-current

The purpose of this study was to discover if individuals taking oral contraceptives differed on measures of sleep and sexual behavior compared to those who are taking no form of birth control. Participants wore motion loggers every night for two-weeks, tracking their sleep, and filled out a questionnaire every morning inquiring about their sleep, sexual feelings, sexual behaviors, and affect from the day before. Research in this area of study is very sparse and results are conflicting, therefore I aimed to obtain comprehensive information to derive a basic understanding on if sexual behavior variables and sleep variables are in fact …

Assessing Structural Variation In And Out Of The Classroom: Characterizing Structural Variation Within Campanula Americana And Effects Of Long Read Sequencing On Students Learning, Tyler Gandee

Masters Theses, 2020-current

Cytonuclear incompatibilities are proposed to be one of the first genetic barriers to arise during speciation, so understanding the underlying evolutionary forces that contribute to cytonuclear incompatibilities is important. Many studies have looked at SNP level variation in the plastid genome and its relationship with cytonuclear incompatibility, but no studies have examined structural variation and its relationship to cytonuclear incompatibility, particularly at the early stages of speciation, i.e. within species. Campanula americana is a great system for this because it exhibits cytonuclear incompatibility and the family in which it is found, the Campanulaceae, exhibits rapidly evolving plastid genomes, including …

Dynamics Of Skin-Based Gene Expression In Red-Sided Garter Snakes (Thamnophis Sirtalis Parietalis): Sex And Seasonal Influences, Emily C. Plant

Masters Theses, 2020-current

The skin is the key organ regulating the animal-environment interface and is particularly sensitive to the abiotic factors of temperate environments, such as extreme cold and aridity during winter. Previous research found that, in the skin, temperate birds and reptiles upregulate expression of lipid synthesis genes to prevent tissue freezing and evaporative water loss in winter. My study species, the red-sided garter snake (Thamnophis sirtalis parietalis), has seasonally variable skin lipid composition, and females produce a modified set of these lipids that function as a sex pheromone. The pheromone is maximally produced in the spring breeding season following …

Assessing The Effectiveness Of Treatment Programs Among Veterans With Opioid Use Disorder In New York State, Olatoye Oluwaseyi Itaniyi

Walden Dissertations and Doctoral Studies

New York State (NYS) is home to millions of veterans and has the fifth-largest veteran population in the country. Veterans represent a vulnerable population and are at higher risk for mental and physical health struggles. The focus of this quantitative study was to assess if there is any significant difference between pre- and post-OUD 24-hour detoxification treatment services, residential/rehabilitation treatment services, ambulatory outpatient treatment services, frequency of opioid use at discharge, and mental disorder among veterans with opioid use disorder in NYS when controlling for age, gender, race, and marital status. The study utilized the harm reduction framework and the …

Case Manager Perceptions Of Trauma-Informed Practice And Policy In Human Service Organizations, Lachandra Brooks Prim

Walden Dissertations and Doctoral Studies

One of the goals of human service organizations is to provide help to victims of trauma, but they are often the cause of retraumatization to those individuals. The problem that was addressed in this study was the negative repercussions of retraumatization such as feelings of being unsafe, anger, guilt, shame, depression, and secondary trauma due to human service organizations not being based in trauma-informed approaches. The purpose of this generic qualitative research study was to describe and understand case managers’ perceptions of trauma-informed practices and policies across organizations and services. Ecological systems theory served as the theoretical framework for this …

Role Of Dunc-13 Isoforms In Alcohol Sensitivity In Drosophila, Makenzie Havard

Honors Theses

Functional alcohol tolerance is an adaptation made by the nervous system to high doses of alcohol, and is seen by a significant reduction in sensitivity to this drug. This tolerance is a significant contributing factor in the progression to Alcohol Use Disorders. A major goal of alcohol research is to understand how alcohol induces this tolerance. Dunc13 is a presynaptic active zone protein essential in synaptic vesicle docking and priming. Alcohol binds to the C1 domain of Dunc13 at physiological concentrations, which inhibits Dunc13 from binding Diacylglycerol. This inhibition is predicted to reduce Dunc13 activity and presynaptic activity. Yet, genetically …

Spin Coating Multilayer Polymer Films To Improve The Consistency And Longevity Of Interval Drug Release, Katherine Nevils

Honors Theses

Treatment-resistant depression (TRD) is a clinical diagnosis for patients suffering from major depressive disorder (MDD). An inadequate response to the dosage or duration of antidepressant medication can lead to the diagnosis of TRD. This diagnosis is common in up to 50% of patients with MDD following treatment. Microdosing of classical psychedelics such as lysergic acid diethylamide (LSD) has been shown to have positive cognitive and therapeutic effects on patients with TRD. However, issues of patient compliance to the complex regimen and abuse/diversion of the schedule I controlled substances remain unresolved. The goal of this project is to create an implantable, …

Creating A Collapsible Sling Lift Capable Of Transporting Patients Between Various Locations, Cameron Delaney Hillman, Macey Jewell Ross, Lydia Noel Miller

Honors Theses

As Senior Biomedical Engineering students at the University of Mississippi, we, Lainey Hillman, Lydia Miller, and Macey Ross, are required to complete a final senior capstone for our department. This project aims to provide a solution to a current unmet clinical need in the healthcare industry, thus our team sought to improve the lack of transportability of sling lifts. The current sling lifts on the market display numerous issues: heavy, bulky, use confined to certain areas, and use specific for only one type of transportation. To combat these problems, we designed a sling lift that was lightweight, collapsible, safe, and …

La Langue Des Autres: The Linguistic Evolution Of African Representation In French Popular Culture, Colonialism To Present, Kathrynanne Eastman

Honors Theses

This thesis examines the linguistic representation of African peoples and cultures in French popular culture, specifically as this pertains to immigration. The foundational research question of this project is: how has the representation of Africans in French popular culture evolved since the colonial period? In order to answer this question, I examine seven sources of popular culture, all works of either literature or cinema, depicting three different time periods: 19th-century French colonization in Algeria (1830-1900), the post-World War II “Trente Glorieuses” [Thirty Glorious Years] (1945 to 1975), and the contemporary era (1990-present). I lay out and analyze the language present …

Guardian's Goals And Gains: The Relationship Between Need Satisfaction And Parenting Goals In Parents Of Toddlers, Kallen E. Mitchell

Honors Theses

Self Determination Theory (SDT) theorizes that when three core psychological needs are met (i.e., competence, relatedness, and autonomy) then humans reach their highest level of optimization (Deci & Ryan, 2000). One common way people tend to satisfy these needs is through social relationships, typically ones where their efforts can be reciprocated; however, not all relationships are reciprocal. This study looks at the psychological needs within an asymmetrical relationship between a parent and their toddler aged child. Given an already established relationship (Smith et al. 2023) between need satisfaction and the types of goals parents have for their college aged kids …

Early Developmental Exposure To Δ9-Tetrahydrocannabinol Causes Latent Behavioral, Growth, And Bioenergetic Effects In Danio Rerio, Astra Hahm

Honors Theses

Early developmental exposure to Δ9-tetrahydrocannabinol causes latent behavioral, growth, and bioenergetic effects in Danio rerio

(Under the direction of Dr. Kristine Willett)

Δ9-tetrahydrocannabinol (Δ9-THC) is used during pregnancy to mitigate symptoms such as nausea, pain, and insomnia. It is established that Δ9-THC exposure early in life causes adverse effects in children’s development including behavioral disorders. Our goal was to determine the latent and generational impacts on growth, behavior, and mitochondrial bioenergetics following exposure to Δ9-THC. Wildtype (5D) zebrafish were exposed to 0 (0.05% DMSO), 0.08, 0.4, or 1 µM Δ9-THC from 6-96 hours post-fertilization (hpf), then subsequently raised in clean …

Into The Wild: A Journey Paddling The Lower Mississippi River With Quapaw Canoe Company, Sarah Caroline Crall

Honors Theses

Humanity’s profound relationship with water is undeniable. From our reliance on it for sustenance and transportation to the recreation it provides, water is a fundamental aspect of our existence. Yet, in our contemporary era, we find ourselves increasingly disconnected from this life-giving source that shapes us and our surroundings. The Mississippi River stands as a prime example of this detachment. Often overlooked as a lifeless river, nothing more than the backdrop to our daily lives, its significance as the heart of America is underappreciated and misunderstood by many. This thesis embarks on a journey to share the true reality that …

The Effects Of Vigorous-Intensity Acute Exercise On Hippocampal- And Prefrontal Cortex-Dependent Memory, Lauren Hord

Honors Theses

Acute exercise has been shown to differentially impact memory, dependent on whether the task is considered prefrontal cortex- or hippocampal-dependent. More specifically, acute exercise has been shown to impair PFC-dependent memory while enhancing hippocampal-dependent memory. No study has compared whether exercise differentially impacts separate types of cognition, so this experiment was designed to determine the relationship between high-intensity acute exercise and both prefrontal cortex-dependent and hippocampal-dependent memory. This study consisted of five total visits for 55 participants. In the first visit, participants engaged in a maximal exercise test, where their heart rate reserve was obtained. In visits 2-5, participants engaged …

Childhood Food Insecurity As It Relates To Adult Intuitive Eating Patterns, Lily Langley

Honors Theses

Many American families struggle with food insecurity and do not have the economic capabilities to obtain a sufficient quantity of nutritious food. Childhood food insecurity is associated with negative health effects and altered relationships with food as an adult. Intuitive eating, a non-diet framework that emphasizes mindful eating, may attenuate these effects. It includes unconditional permission to eat, eating for physical rather than emotional reasons, reliance on hunger and satiety cues, and body-food choice congruence. Previous research has not examined whether childhood food insecurity can affect one’s ability to eat intuitively as an adult. The goal of this study was …

Fe(Iii) And Mn(Ii) Binding De Novo Designed Peptides And Their Oxidation With H2o2, Hannah Randall, Russ Upton

Honors Theses

Metalloenzymes perform a wide variety of reactions that are instrumental to the basic functions of life. Learning more about those enzymes–their structure, folding, active sites, and co-factors–can help to not only better understand the finer details of the chemical world, but also help provide answers and possible solutions to questions pertaining to alternative energy. Particulate Methane Monooxygenase (pMMO) is a metalloenzyme that oxidizes methane into methanol in the biological processes of some methanogenic bacteria, a valuable part of their process to obtain energy. One of its active sites, CuB, has a relatively unknown function. Heptad repeats of model enzymes have …

Science Quest! An Educational Comic For Fifth Graders In Mississippi, Astra Hahm

Honors Theses

Astra Hahm: Science Quest! An educational comic for fifth graders in Mississippi (Under the direction of Dr. Joshua Eyler)

Science Quest! is an educational comic aimed at fifth graders in Mississippi to teach them science in a way that differs from how they learn science in class. The inspirations for the creation of Science Quest! are both my interests as a child and my vision of the future as an adult. During Sophomore year, I had the privilege of volunteering at Oxford Intermediate School, where I witnessed students play a variety of educational games, which reminded me of my childhood. …

Detection Of Sars-Cov-2 Nucleocapsid And Spike Antibodies In Serum Of Covid-19 Vaccinated And Infected Patients Utilizing Immunoblot Assay, Suzanna Horn

Honors Theses

In 2020, the world was introduced to COVID-19, a strain of Coronavirus that quickly caused a pandemic at the global scale. COVID-19 was reported to cause 5.94 million deaths between 2020-2021, and it continues to prevail amongst society in 2024. Many methods of preventing COVID-19 infection arose throughout this time period; however, mRNA vaccines against the SARS-CoV-2 spike (S) protein became the most effective way to protect the population from COVID-19’s destructive reign.

Although society has made significant scientific strides in Coronavirus testing and vaccination efforts, there are many improvements that must be made to better protect our society from …

Validation Of A Triple Quadrupole Lcms Instrument For Cocaine And Amphetamines, Isabel R. Nichols

Honors Theses

Despite the opioid crisis taking headlines, the abuse of cocaine and amphetamines remains rampant and laboratory instruments must be able to effectively and reliably handle the overwhelming case load. In this study, an Agilent 6475 Triple Quadrupole (QQQ) LCMS, recently acquired by the Tarrant County Medical Examiner’s Office (TCMEO) in Fort Worth, Texas, was validated for identifying and quantifying CocAmps. Results show that the instrument was fit-for-purpose and that the instrument passed all validation tests. Reproducibility was ≤ 20% RSD for 12 QC replicates, 6 of blood and 6 of urine. Accuracy was ± 20%. A 98.9% recovery was yielded …

Functional Connectivity Of The Hippocampus In Autism Spectrum Disorder, Noah Watts

Honors Theses

Autism spectrum disorder (ASD) is a neurological developmental disorder that affects around 1/44 of individuals in the United States. ASD individuals tend to struggle with things such as communication and socialization. This disorder has been found to affect many brain regions; however, autism’s effect on the hippocampus has been heavily understudied. Since the hippocampus is not commonly referred to as the brain region affected by autism, the importance of the hippocampus in ASD may have been unrecognized. While ASD hippocampi have been very narrowly researched (only size and shape), there is compelling evidence that the hippocampus could be another part …

Casi: A Framework For Cross-Timepoint Analysis Of Single-Cell Rna Sequencing Data, Yizhuo Wang, Christopher R Flowers, Michael Wang, Xuelin Huang, Ziyi Li

Faculty, Staff and Student Publications

Single-cell RNA sequencing (scRNA-seq) technology has been widely used to study the differences in gene expression at the single cell level, providing insights into the research of cell development, differentiation, and functional heterogeneity. Various pipelines and workflows of scRNA-seq analysis have been developed but few considered multi-timepoint data specifically. In this study, we develop CASi, a comprehensive framework for analyzing multiple timepoints' scRNA-seq data, which provides users with: (1) cross-timepoint cell annotation, (2) detection of potentially novel cell types emerged over time, (3) visualization of cell population evolution, and (4) identification of temporal differentially expressed genes (tDEGs). Through comprehensive simulation …