Digital Commons Network^™

Imaging Features Of Primary Intracranial Sarcoma With Dicer1 Mutation: A Multicenter Case Series, Rami W Eldaya, Richard J Fagan, Samir A Dagher, Angshumoy Roy, Sonika Dahyia, Gregory N Fuller, Max Wintermark, Matthew S Parsons, Thierry A G M Huisman

Faculty, Staff and Student Publications

Primary intracranial sarcoma, DICER1-mutant, is a rare, recently described entity in the fifth edition of the WHO Classification of CNS Tumors. Given the entity's rarity and recent description, imaging data on primary intracranial sarcoma, DICER1-mutant, remains scarce. In this multicenter case series, we present detailed multimodality imaging features of primary intracranial sarcoma, DICER1-mutant, with emphasis on the appearance of the entity on MR imaging. In total, 8 patients were included. In all 8 patients, the lesion demonstrated blood products on T1WI. In 7 patients, susceptibility-weighted imaging was obtained and demonstrated blood products. Primary intracranial sarcoma, DICER1-mutant, …

Vista: May 9, 2024, University Of San Diego

USD Vista (1965-)

No abstract provided.

Overcoming Challenges: Management Of Dentat Implants In The Aesthetic Zone, Blake Giuliani

Excellence Day

Project Summary The patient was seen by two different student dentists before being transferred to me. I came to assist with the implant surgery and continued dental care from there on. The patient presented with missing #8 and a large vertical bone defect from #7-#9. The patient's confidence had gotten worse due to the continuous failure of implants and missing a front tooth. After over 3 years of treatment at the school, the patient felt whole again. Significance: Vertical bone defects can be very hard to manage, but espcially hard to manage when they are in the anterior region. Some …

Attempting Heroics: Restoring Buccally Angulated Implants, Ryan Leong

Excellence Day

Buccally placed tissue-level implants in the esthetic zone may encounter various challenges, particularly concerning the trajectory of the screw channel and the implant body's placement. In this case, while addressing an edentulous space in the upper left quadrant with an implant bridge spanning from #12 to #14 it became evident that #12 was far too buccally placed. To address this issue, #14 was designed for screw retention, while #12 was planned for cement retention to accommodate the screw channel exiting through the buccal cusp. Although this method may seem to make the cement retained aspect of the restoration unretrievable, using …

Restoring A Smile: Functional And Esthetic Full Mouth Rehabilitation, Rachel Min

Excellence Day

Summary of Project: Restoring vertical dimension of occlusion (VDO) involves a complex interdisciplinary approach involving multiple specialties. The patient presented to the clinic with loss of VDO, tooth structure, and multiple posterior teeth leading to a smile and function that she was unsatisfied with. After careful planning, we created a treatment plan that would be carried out in multiple phases: increasing VDO through anterior direct restorations, endodontic treatment, placing implants, and restoring her posterior occlusion through indirect restoration of multiple premolars and implant crowns. This case demonstrates the detailed steps, treatment planning, and care provided to achieve a stable occlusion …

Dental Pulp Stem Cells In Regenerative Dentistry, Zachary Eschenberg, Marie Tolarova, Mirek Tolar

Excellence Day

Abstract Objectives: This literature review aims to provide a comprehensive overview of recent research on dental pulp stem cells (DPSCs) in regenerative dentistry, addressing their characteristics, importance, isolation techniques, utilization in different dental specialties, and future prospects. Methods: Relevant studies published within the last 20 years were identified through database searches and manual selection. The review synthesizes findings from key research articles, including those investigating DPSC characterization, isolation methods, therapeutic applications, and future uses. Results: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located within dental pulp tissue, exhibiting a high proliferation rate and multilineage differentiation capacity. DPSCs offer …

Management Of Pain In Orthodontics, Brianna Wolfe, Gregory Pavlos, Miroslav Tolar, Marie Tolarova

Excellence Day

Objectives: The objective of this literature review is to explore the current studies on orthodontic pain management amongst various orthodontic treatment approaches (fixed appliances, clear aligner therapy, expansions, temporary skeletal anchorage devices, etc.), and to analyze the efficacy of existing and new interventions for patients experiencing orthodontic pain. Methods: Search engines, including PubMed and Google Scholar, were employed, using keywords of orthodontic pain, orthodontic pain management, orthodontics AND pain, with NCBI filters of Free Full Text, Clinical Trial, Meta-Analysis, Randomized Controlled Trial, and Systematic Review. A time restriction from 2007-2024 and English language were applied. Results: After rigorous restrictions a …

Zirconia Vs Pfm In Long Span Bridges, Puneet Datewas, Sithara Nair, Hamza Tariq

Excellence Day

Objectives: 1. Compare the basic properties of Zirconia and Porcelain-Fused-to-Metal (PFM) in dental prostheses. 2. Assess the rates of technical complications like fracture, chipping and biological outcomes between Zirconia and PFM prosthesis. 3. Investigate the survival rates of Zirconia-based fixed dental prosthesis (FDPs) versus PFM FDPs. 4. Evaluate the suitability of Zirconia and PFM for long-span bridges, considering strength, durability, aesthetics and its effects. Methods: 1. Conducted systematic research on peer-reviewed articles from websites such as embase, pubMed, google scholar and ovid medline comparing Zirconia Vs PFM. 2. Selected studies evaluating mechanical properties, aesthetics, biocompatibility, clinical outcomes and survival rates. …

Histological And Immunohistochemical Observations Of The Hippocampus In Clinically Established Neurodegenerative Disorders Linked To Cause Of Death, Joshua Owolabi, Jacquelyn Gerhart, Rasheed Agboola, Steven Morency, Brian J. Balin Phd

Research Day

Introduction: Neurodegeneration remains a very important subject of neuroscience research. While experimental studies have modeled conditions and attempted to provide insights, special opportunities exist in situations where clinically established conditions in the actual human brain can be investigated for the neurological, glial and molecular changes that characterize brain changes that produce pathological and neuronal aberrations that are manifested in these conditions. This study considers hippocampi in brains that have suffered clinically established neurodegeneration leading to death.

Methods: Twelve hippocampus samples [N=12] were obtained from 6 brains with clinically established cause of death being neurodegenerative disorders. In each case, whole hippocampi …

Comparative Life Cycle Assessment Of Hydrogen Production Via Various Pv-Assisted Electrochemical Water Splitting Techniques, Achyuth Ravilla, Ilke Celik

Student Research Symposium

Photoelectrochemical (PEC) and photovoltaic-electrochemical (PV-EC) water-splitting technologies have emerged as cost-effective options for large-scale green hydrogen production in industrial applications. Solar to hydrogen (STH) efficiencies of these technologies have reached up to 20% and several pathways have been explored to drive down the cost of hydrogen using these technologies to less than $2/kg. However, the environmental impact assessment of these technologies for industry-scale deployment has not been explored in previous studies. This study assesses the environmental impacts of PEC and PV-EC technologies by conducting a cradle-to-gate life cycle assessment. The functional unit considered for this assessment is 1 kg of …

Hell’S Tails: Localizing The Tail Proteins Of The Hyperthermophilic-Infecting Virus Ssv1, Jane M. Arterberry

Student Research Symposium

The tail of Saccharolobus spindle-shaped virus 1 (SSV1) has long been thought to be composed of viral protein 4 (VP4), but recent SSV1 virion structural studies have implicated additional structural proteins C166 and B78 in SSV1’s unique tail. The exact locations of these proteins have not been confirmed. Structural proteins VP4 (C-terminus) and B78 are hypothesized to contribute to the adaptor region bridging the viral capsid to the tail, whereas C166 is thought to comprise the majority of the internal extended structure, respectively. The structural proteins comprising SSV1 lack cysteine residues. Mutations were made in the open reading frames of …

Mutagenetic Analysis Of The Minor Capsid Protein Vp3 Of The Archaeal Fusellovirus Ssv1, Laura Costal Lagarde

Student Research Symposium

Viruses, while often associated with disease, are integral to life and play an important role in the microbiome. Despite their importance in medical research, archaeal viruses, particularly those found in extreme environments, are not widely understood. The Sulfolobus Spindle-Shaped Viruses (SSVs) of the Fuselloviridae family are unique among viruses in that they do not kill their host, the archaeon Sulfolobus Solfataricus in volcanic hot springs, but rather slow its growth, allowing for halo assay assessments.The study focuses on the type virus SSV1, which is predominantly composed of the Major and Minor capsid proteins vp1 and vp3, respectively. Previous study has …

Relationship Between Trunk Cross-Sectional Area Growth And Water Stress In Garry Oaks (Q. Garryana): A Species Of Conservation Concern, John Cochrane

Student Research Symposium

In the Pacific Northwest, Quercus garryana (Garry oak trees) support over 627 native species. Garry oak natural habitat was originally maintained by indigenous practices but has been reduced to 5-10% of its range due to change in land management strategies. To support oak conservation, we need to understand heat and water affect the growth and physiology of this species. In this study, we created a fixed linear model of trunk cross-sectional growth with water stress (Ψ), photosynthetic water-use efficiency (δ¹³C), and leaf Carbon-Nitrogen ratio. We collected branch samples from the canopies of 47-64 mature Garry oaks in the …

Describing Shen & Shen Ming Via A Hybrid Quantum Computational Model, Alex M. Cullen, Johnny Lemau

Student Research Symposium

Definitions for Traditional Chinese Medicine (TCM) terminology within the context of quantum biology (QB) have recently been proposed. These definitions aim to elucidate concepts such as qi meridians in TCM. Our study presents a hypothesis that seeks to provide an operational definition for Spirit (Shen) and Bright Spirit (Shen Ming) in TCM via a scoping review. We propose that Shen represents physical, phenomenological proto-consciousness/consciousness in the forms of computation, such as singular particles/bits/qubits, computers, organisms, or computational strategies, whereas Shen Ming may refer to emergent, quasi-physical meta-proto-consciousness/consciousness formed by coherent relationships of multiple orchestrated/integrated units of computation; manifesting itself as …

Expressive And Instrumental Social Capital Facilitates Network Connections For Community College Transfer Students In Stem, Makenna R. Stone

Student Research Symposium

Students that transfer from a community college (CC) to a university often experience social and academic barriers that can lead to feelings of isolation and challenge their persistence. One way to alleviate these barriers is to develop capital. We aimed to understand how a cohort of CC STEM transfer students may have developed and exhibited social capital, or “assets accessed through social connections”. We leveraged the Network Theory of Social Capital to examine the instrumental (i.e., concrete advice and support) and expressive (i.e., emotional support and encouragement) actions that led to feelings of connection and persistence. We conducted semi-structured interviews …

Improved Genome Maintenance And Dna Replication In The Anoxia Tolerant Annual Killifish, Riley A. Roth-Carter

Student Research Symposium

Timely and faithful replication of the genome is a requirement for cell survival and proliferation, with errors in this process leading to cancers and cell death. DNA replication during exposure to stressful conditions can lead to increased mutational burden, with collapsed replication forks causing mutations leading to cancers due to loss of repair capabilities during these exposures. Learning how stressful DNA replication takes places can lead to a better understanding of how resistant cancers survive similar conditions, like hypoxic tumor microenvironment, direct irradiation, and DNA damaging chemotherapeutics. Using an extremophile model, Austrofundulus limnaeus, which can survive these genotoxic stressors could …

The Longitudinal Relationship Between Socioeconomic Status, Child Separation Anxiety Symptoms, And School Achievement In 1st Grade, Filip-Bogdan Serban-Dragan

Student Research Symposium

This informative poster highlights a study examining the association between child separation anxiety disorder (SAD) and school achievement, considering socioeconomic status (SES) and perceived financial stress. Data came from the Early Growth and Development Study (EGDS). Data from the kindergarten (age 6) and 1^st grade (age 7) assessments in the adoptive families (N=360 adoptive triads) were analyzed.

By controlling for parent’s income, financial stress, child’s gender and by measuring children at two time points (6 and 7 years), our study aims to identify the specific contribution of separation anxiety on academic achievement, enhancing our understanding of this …

Examining The Role Of Rgs2 In The Maintenance Of Diapause After Anoxic Stress In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Patrick Clouser

Student Research Symposium

Austrofundulus limnaeus live in ephemeral ponds of Venezuela. They have evolved a unique life history that includes embryonic diapause, a period of developmental dormancy, metabolic arrest, and reduced protein synthesis. Diapausing embryos are resistant to environmental stress and survive months without oxygen (anoxia). A. limnaeus’ anoxia tolerance is an important survival mechanism as they can be buried in anoxic soil during development. Analysis of RNAseq data from diapausing A. limnaeus embryos show significant down-regulation of RGS2 transcripts in anoxic embryos. RGS2 regulates G-protein receptor signaling by inhibiting associated G_a-proteins and can inhibit protein synthesis. I hypothesis that …

Neuroscience Educational Interventions For Mental Health Management Within The Neurodiverse Population: A Working Model, Kadi Rae Smith, C.K. Miller, Bill Griesar, Jeff Leake

Student Research Symposium

Sociological and environmental factors such as economic disparity and cultural marginalization hinder access to educational and medical resources and must be taken into account to ensure accurate diagnoses, effective self-regulation and mental health management for neurodiverse individuals, and offset disparities in access to healthcare and education. Arbitrary attachment of stigma to traits commonly associated with neurodivergence perpetuate neuro-myths1 that have dire consequences when internalized, informing individuals’ development and sense of self. Neuroscience outreach done by NW Noggin is an active model for engaging communities with limited resources and a greater need for services to offset economic disparity. Integrated arts programming …

Locating Vitamin D Receptors (Vdrs) In Annual Killifish, Austrofundulus Limnaeus, Rosalia N. Tanori, Amie L. Romney, Jason E. Podrabsky

Student Research Symposium

Austrofundulus limnaeus, a species of annual killifish found in unpredictable temporary habitats in South America, has a profound ability to survive long periods without water and oxygen. This tolerance is associated with the ability to enter metabolic dormancy associated with diapause. Entrance into diapause and tolerance of environmental stress is governed by an interplay of genetic and environmental factors. The vitamin D receptor (VDR) is among these factors and plays a crucial role in determining if an embryo will enter diapause or actively develop. My work aims to identify the genomic locations of VDR proteins in the A. limnaeus genome …

Construction And Mutagenesis Of Ssv1 Mutants In Extreme Viruses, Lou Ann F. O'Connor, Jono Abshier, Kenneth M. Stedman

Student Research Symposium

Archaeal viruses with unique structures such as spindle-shaped virions are found abundantly in extreme environments like geothermal hot springs around the world. Among all spindle-shaped viruses, the model Sulfolobus Spindle-shaped Virus 1 (SSV1) is best studied. Creating the lemon-shaped or spindle-shaped virion structure are two proteins, VP1 as the major capsid protein, and VP3 as the minor capsid protein. The primary structure of VP1 consists of a proteolytic cleavage site at position 66 that is believed to be necessary for virus evolution. Recent studies showed that genetic mutation of the amino acid, glutamate (E) at position 66 in VP1 which …

Impact Of Intersecting Vulnerabilities On Functional Outcome And Health-Related Quality Of Life After Traumatic Brain Injury: A Prospective Cohort Study, Rekesh Subedi, Tatiana Hoyos-Gomez, Luis Tinoco-Garcia, John Cervantes Cervantes

Student Research Symposium

Traumatic Brain Injury (TBI) is a critical public health issue that disproportionately affects select vulnerable populations, including race/ethnic minorities, low-income individuals, and those without health insurance. Despite high incidences of TBI and its considerable impact on morbidity and mortality, research is limited in addressing how the intersectionality of vulnerabilities contributes to outcomes post-TBI. This literature review aims to synthesize existing knowledge regarding Social determinants of health (SDH) on TBI outcomes, focusing on functional recovery and health-related quality of life (HRQOL). This study will use a prospective cohort research design where TBI patients at Oregon Health and Science University (OHSU) will …

Exploring Factors That Contribute To Stem Transfer Student Sense Of Belonging, Brittney R. Gallant, Cate Pocitari

Student Research Symposium

There is an ongoing disconnect between the number of students who enter college intending to earn a STEM degree and the number of students who persist to degree, this is particularly true for transfer students. Sense of belonging has been named as a STEM persistence factor, yet how that belonging develops for transfer students is largely unknown. Here we used a mixed-methods design to explore the experiences of STEM transfer students at PSU (n=38). Participants completed a survey prior to participating in an interview. The survey and interviews explored their science identity, sense of belonging, and involvement in STEM-related activities. …

Histone Changes As A Response To Lactic Acid Selection In Cells Of Austrofundulus Limnaeus, Lori Tran, Jason Podrabsky, Chelsea Hughes

Student Research Symposium

The killifish Austrofundulus limnaeus is an extremophile native to small temporary ponds of Venezuela. Normal embryonic development of A. limnaeus is segmented into up to three stages of metabolic depression (diapause), and both developing and diapausing embryos can survive long bouts of the complete absence of oxygen (anoxia) by using anaerobic metabolic pathways. While there has been much focus on how mutations in DNA drive evolution, environmental stress can cause heritable changes to histone post-translational modifications (hPTMs) in multiple species. However, this evolutionary role of hPTMs has never been studied in A. limnaeus. Additionally, the possible evolutionary role of extracellular …

Qibc Analysis On Killifish Cells Under Anoxic Conditions, Jaina Canlas

Student Research Symposium

Faithful DNA replication is vital for all living organisms. Environmental stressors can cause a multitude of mutations and DNA lesions during replication leading to cell death if not accurately repaired. The annual killifish, Austrofundulus limnaeus, has a heightened tolerance to many known genotoxic stressors, including anoxia. Embryos of the annual killifish can survive months of anoxia during the dry season when the ephemeral ponds they inhabit, completely dry. During these exposures, they have shown they are capable of repairing substantial amounts of damaged DNA. This resilience has led us to hypothesize that A. limnaeus have enhanced DNA repair capabilities. …

Development Of A Two-Photon Laser Scanning Microscope, Jess Hollenbaugh

Student Research Symposium

The objective of this project was to convert a Sarastro 2000 confocal laser scanning microscope into a system capable of imaging using two-photon excitation (TPE) fluorescence for the use of the PSU biology department. TPE microscopy operates on the ability of fluorophores to accept two photons each with half the energy of a desired transition in a single quantum event via a virtual energy state and then emit a higher energy photon upon relaxation. This is preferable to single-photon excitation (SPE) due to the lower energy photons causing less damage to delicate biological samples. The adaptation process included physically altering …

Annual Killifish: Eye Development And Retinal Cell Regeneration, Carmen Z. Rodriguez

Student Research Symposium

Vertebrate eye development is highly conserved. Current models have allowed the understanding of gene expression patterns that support eye development, but lack the context of extreme environmental conditions that challenge these fundamental programs. Typical vertebrate life spans can be years, but annual killifish, Austrofundulus limnaeus, are able to complete their entire adult lifespan in only weeks. Thus, A. limnaeus is a strong model for age-related macular degeneration. A. limnaeus also experiences embryonic diapause; a period of developmental dormancy. Developing/diapausing embryos of A. limnaeus are resistant to environmental stresses such as hypoxia/anoxia that would cause irreparable damage to vital organs, such …

Guide Rna Design And Delivery For Crispr/Cas9 Editing In Annual Killifish, Keria N. Moritsugu-Vandehey, Isabel Henkes, Yekaterina Chmykh, Amie Romney, Jason Podrabsky

Student Research Symposium

The CRISPR-Cas9 genome editing tool has shown to be successful in knocking out genes in model organisms such as zebrafish, turquoise killifish, and cichlid fish. CRISPR-Cas9 genome editing has been demonstrated in many species of fish, but this technology has not been verified in the annual killifish, Austrofundulus limnaeus. We hypothesize that targeted editing of the tyrosinase gene in embryos of A. limnaeus would lead to the development of fish without the ability to produce melanin, the black/brown pigment molecule. Early embryos (1-cell stage) were injected with a Cas9 cocktail containing a mix of guide RNA molecules that target …

Selection During Reproduction In Mimulus Guttatus, Desmond C. Willson, Karla De Lima Berg, Mitch Cruzan

Student Research Symposium

A lack of genomic studies examining gametophytic selection and selective embryo abortion—which occur during the reproduction of angiosperms—leaves questions regarding the adaptive and evolutionary effects of these processes. Analyzing deviations from Mendelian segregation offers an avenue for identifying loci targeted by GS and SEA, and their contributions to purging of genetic load. However, other selective processes such as meiotic drive and cytonuclear interactions, as well as pollen and ovule abortion, can cause distortion. To distinguish the effects of GS and SEA from other causes of distortion, we will perform reciprocal crosses between highly homozygous and highly heterozygous individuals of Mimulus …

Pulse Modulation In Braided Pneumatic Actuators Mimics Contractile Behavior Of Biological Muscles, Mohamad Elzein

Student Research Symposium

Advancements in robotics and bioengineering aim to emulate biological muscle systems through robotic actuators, blending mechanical strength with biological adaptability. A lesser-explored aspect is mimicking the pulse-like control characteristic of biological muscles, which contract in response to action potentials from motoneurons, with muscle contractile force relying heavily on the timing between these potentials. This study explores the effect of pulse lengths and the gaps between pulses on braided pneumatic actuators (BPAs), which mimic the nonlinearity and dynamic response of biological muscles. It hypothesizes that artificial muscles utilizing pulse-based control will exhibit a similar force dependency on the intervals between pulses …