Digital Commons Network^™

Targeting Il-11r/Ezh2 Signaling Axis As A Therapeutic Strategy For Osteosarcoma Lung Metastases, Eswaran Devarajan, R Eric Davis, Hannah C Beird, Wei-Lien Wang, V Behrana Jensen, Arumugam Jayakumar, Cheuk Hong Leung, Heather Y Lin, Chia-Chin Wu, Stephanie A Ihezie, Jen-Wei Tsai, P Andrew Futreal, Valerae O Lewis

Faculty, Staff and Student Publications

Lung metastases are the primary cause of death for osteosarcoma (OS) patients. We recently validated interleukin-11 receptor α (IL-11Rα) as a molecular target for the inhibition of OS lung metastases. Since there is no clinically approved antibody against this receptor, we sought to identify downstream targets that mediate the effects of IL-11Rα signaling. We used shRNA to deplete IL-11Rα from OS cells; as a complementary approach, we added IL-11 exogenously to OS cells. The resulting changes in gene expression identified EZH2 as a downstream candidate. This was confirmed by knockdown of IL-11Rα in OS cells, which led to increased expression …

Ocean Rain Detection And Wind Retrieval Through Deep Learning Architectures On Advanced Scatterometer Data, Matthew Yoshinori Otani Mckinney

Theses and Dissertations

The Advanced Scatterometer (ASCAT) is a satellite-based remote sensing instrument designed for measuring wind speed and direction over the Earth's oceans. This thesis aims to expand and improve the capabilities of ASCAT by adding rain detection and advancing wind retrieval. Additionally, this expansion to ASCAT serves as evidence of Artificial Intelligence (AI) techniques learning both novel and traditional methods in remote sensing. I apply semantic segmentation to ASCAT measurements to detect rain over the oceans, enhancing capabilities to monitor global precipitation. I use two common neural network architectures and train them on measurements from the Tropical Rainfall Measuring Mission (TRMM) …

Fate-Mapping And Functional Dissection Reveal Perilous Influence Of Type I Interferon Signaling In Mouse Brain Aging, Ethan R Roy, Sanming Li, Sepideh Saroukhani, Yanyu Wang, Wei Cao

Faculty, Staff and Student Publications

Background: Aging significantly elevates the risk of developing neurodegenerative diseases. Neuroinflammation is a universal hallmark of neurodegeneration as well as normal brain aging. Which branches of age-related neuroinflammation, and how they precondition the brain toward pathological progression, remain ill-understood. The presence of elevated type I interferon (IFN-I) has been documented in the aged brain, but its role in promoting degenerative processes, such as the loss of neurons in vulnerable regions, has not been studied in depth.

Methods: To comprehend the scope of IFN-I activity in the aging brain, we surveyed IFN-I-responsive reporter mice at multiple ages. We also examined 5- …

Repeat Controlled Human Plasmodium Falciparum Infections Delay Bloodstream Patency And Reduce Symptoms, Patricia Ferrer, Andrea A Berry, Allison N Bucsan, Surendra K Prajapati, Karthik Krishnan, Michelle C Barbeau, David M Rickert, Sandra Mendoza Guerrero, Miho Usui, Yonas Abebe, Asha Patil, Sumana Chakravarty, Peter F Billingsley, Faith Pa'ahana-Brown, Kathy Strauss, Biraj Shrestha, Effie Nomicos, Gregory A Deye, B Kim Lee Sim, Stephen L Hoffman, Kim C Williamson, Kirsten E Lyke

Faculty, Staff and Student Publications

Resistance to clinical malaria takes years to develop even in hyperendemic regions and sterilizing immunity has rarely been observed. To evaluate the maturation of the host response against controlled repeat exposures to P. falciparum (Pf) NF54 strain-infected mosquitoes, we systematically monitored malaria-naïve participants through an initial exposure to uninfected mosquitoes and 4 subsequent homologous exposures to Pf-infected mosquitoes over 21 months (n = 8 males) (ClinicalTrials.gov# NCT03014258). The primary outcome was to determine whether protective immunity against parasite infection develops following repeat CHMI and the secondary outcomes were to track the clinical signs and symptoms of malaria and anti-Pf antibody …

A Modelled Estimate Of Food Access Within Countries Shows That Inequality Within Countries Has Increased Despite Rising Equality Between Countries, Juan Diego Martinez, Navin Ramankutty, Zia Mehrabi, Tom W. Hertel

Department of Agricultural Economics Faculty Publications

Global food calorie supply per person is more than 2900 kcal per day on average, but we have failed to ensure equitable access to these calories. Yet how uneven food access is within countries has remained poorly understood, as has the progress made over the past five decades on closing the access gap. Using publicly available data, we developed a theoretically-grounded statistical model to estimate the cross-national relationship between average per capita expenditure and per capita food availability and used this to estimate within-country access to food for income deciles in 135 countries. We find that, from 1961 to 2013, …

Evaluation Of Resistance Patterns And Bioremoval Efficiency Of Hydrocarbons And Heavy Metals By The Mycobiome Of Petroleum Refining Wastewater In Jazan With Assessment Of Molecular Typing And Cytotoxicity Of Scedosporium Apiospermum Jaz-20, Fuad Ameen, Mohammad J. Alsarraf, Tarad Abalkahail, Steven Lee Stephenson

Biological Sciences Faculty Publications and Presentations

Jazan Industrial Economic City (JIEC) is located on the Red Sea coast in the province of Jazan, southwest of Saudi Arabia anchors diverse heavy and secondary industries in the energy, water desalination, petroleum, aluminum, copper, refineries, pharmaceuticals and food manufacturing fields. These various industries generate a large quantity of industrial wastewaters containing various toxicants. The present work represents ecologically beneficial alternatives for the advancement of environmental biotechnology, which could help mitigate the adverse impacts of environmental pollution resulting from petroleum refining effluents. The mycobiome (32 fungal strains) isolated from the industrial wastewater of the refinery sector in Jazan were belonged …

Oregon State Rank Assessment For Western Toad (Anaxyrus Boreas), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Western toad (Anaxyrus boreas) using NatureServe methodology, 2024.

Oregon State Rank Assessment For Golden Eagle (Aquila Chrysaetos), Misty Nelson, Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Golden Eagle (Aquila chrysaetos) using NatureServe methodology, 2024.

The Relationship Between Rejection Sensitivity And Higher Order Cognitive Functions, Rachel Gaynor

USF Tampa Graduate Theses and Dissertations

Rejection sensitivity indexes individual differences in the expectation of, reaction to, and interpretation of possible rejection. Research on mechanisms underlying these individual differences have identified a reduction in top-down emotion regulatory processes in those exhibiting heightened rejection sensitivity. Most of these studies utilize rejection-specific affective stimuli. This study examined the relationship between rejection sensitivity score, calculated from the 18-question RSQ, and the recruitment of higher level inhibition resources, as indexed by the conflict N2, on three novel Flanker tasks. In order to further examine previously identified patterns in affective inhibition across differing levels of rejection sensitivity, and to determine whether …

Real World Predictors Of Response And 24-Month Survival In High-Grade Tp53-Mutated Myeloid Neoplasms, Amandeep Kaur, Alexandra E Rojek, Emily Symes, Mariam T Nawas, Anand A Patel, Jay L Patel, Payal Sojitra, Barina Aqil, Madina Sukhanova, Megan E Mcnerney, Leo P Wu, Aibek Akmatbekov, Jeremy Segal, Melissa Y Tjota, Sandeep Gurbuxani, Jason X Cheng, Su-Yeon Yeon, Harini V Ravisankar, Carrie Fitzpatrick, Angela Lager, Michael W Drazer, Caner Saygin, Pankhuri Wanjari, Panagiotis Katsonis, Olivier Lichtarge, Jane E Churpek, Sharmila B Ghosh, Ami B Patel, Madhu P Menon, Daniel A Arber, Peng Wang, Girish Venkataraman

Faculty, Staff and Students Publications

Current therapies for high-grade TP53-mutated myeloid neoplasms (≥10% blasts) do not offer a meaningful survival benefit except allogeneic stem cell transplantation in the minority who achieve a complete response to first line therapy (CR1). To identify reliable pre-therapy predictors of complete response to first-line therapy (CR1) and outcomes, we assembled a cohort of 242 individuals with TP53-mutated myeloid neoplasms and ≥10% blasts with well-annotated clinical, molecular and pathology data. Key outcomes examined were CR1 & 24-month survival (OS24). In this elderly cohort (median age 68.2 years) with 74.0% receiving frontline non-intensive regimens (hypomethylating agents +/- venetoclax), the overall cohort CR1 …

Phase 2 Study Of Neoadjuvant Enzalutamide And Paclitaxel For Luminal Androgen Receptor-Enriched Tnbc: Trial Results And Insights Into “Arness”, Bora Lim, Sahil Seth, Clinton Yam, Lei Huo, Takeo Fujii, Jangsoon Lee, Roland Bassett, Sara Nasser, Lisa Ravenberg, Jason White, Alyson Clayborn, Gil Guerra, Jennifer K Litton, Senthil Damodaran, Rachel Layman, Vicente Valero, Debasish Tripathy, Michael Lewis, Lacey E Dobrolecki, Jonathan Lei, Rosalind Candelaria, Banu Arun, Gaiane Rauch, Li Zhao, Jianhua Zhang, Qingqing Ding, W Fraser Symmans, Jeffrey T Chang, Alastair M Thompson, Stacy L Moulder, Naoto T Ueno

Faculty, Staff and Student Publications

Luminal androgen receptor (LAR)-enriched triple-negative breast cancer (TNBC) is a distinct subtype. The efficacy of AR inhibitors and the relevant biomarkers in neoadjuvant therapy (NAT) are yet to be determined. We tested the combination of the AR inhibitor enzalutamide (120 mg daily by mouth) and paclitaxel (80 mg/m2 weekly intravenously) (ZT) for 12 weeks as NAT for LAR-enriched TNBC. Eligibility criteria included a percentage of cells expressing nuclear AR by immunohistochemistry (iAR) of at least 10% and a reduction in sonographic volume of less than 70% after four cycles of doxorubicin and cyclophosphamide. Twenty-four patients were enrolled. Ten achieved a …

Structural Basis For Hyperpolarization-Dependent Opening Of Human Hcn1 Channel, Verena Burtscher, Jonathan Mount, Jian Huang, John Cowgill, Yongchang Chang, Kathleen Bickel, Jianhan Chen, Peng Yuan, Baron Chanda

2020-Current year OA Pubs

Hyperpolarization and cyclic nucleotide (HCN) activated ion channels are critical for the automaticity of action potentials in pacemaking and rhythmic electrical circuits in the human body. Unlike most voltage-gated ion channels, the HCN and related plant ion channels activate upon membrane hyperpolarization. Although functional studies have identified residues in the interface between the voltage-sensing and pore domain as crucial for inverted electromechanical coupling, the structural mechanisms for this unusual voltage-dependence remain unclear. Here, we present cryo-electron microscopy structures of human HCN1 corresponding to Closed, Open, and a putative Intermediate state. Our structures reveal that the downward motion of the gating …

Protein Restriction Slows The Development And Progression Of Pathology In A Mouse Model Of Alzheimer's Disease, Reji Babygirija, Natalie M Niemi, Et Al.

2020-Current year OA Pubs

Dietary protein is a critical regulator of metabolic health and aging. Low protein diets are associated with healthy aging in humans, and dietary protein restriction extends the lifespan and healthspan of mice. In this study, we examined the effect of protein restriction (PR) on metabolic health and the development and progression of Alzheimer's disease (AD) in the 3xTg mouse model of AD. Here, we show that PR promotes leanness and glycemic control in 3xTg mice, specifically rescuing the glucose intolerance of 3xTg females. PR induces sex-specific alterations in circulating and brain metabolites, downregulating sphingolipid subclasses in 3xTg females. PR also …

Incidence And Risk Factors For New And Recurrent Infarcts In Adults With Sickle Cell Disease, Lori C Jordan, Allison A King, Andria L Ford, Taniya E Varughese, Lisa Garrett, Et Al.

2020-Current year OA Pubs

BACKGROUND: Most adults with sickle cell disease will experience a silent cerebral infarction (SCI) or overt stroke. Identifying patient subgroups with increased stroke incidence is important for future clinical trials focused on stroke prevention. Our 3-center prospective cohort study tested the primary hypothesis that adults with sickle cell disease and SCIs have a greater incidence of new stroke or SCI compared with those without SCI. A secondary aim focused on identifying additional risk factors for progressive infarcts, particularly traditional risk factors for stroke in adults.

METHODS AND RESULTS: This observational study included adults with sickle cell disease and no history …

Selected Essays On Family Law (Committee Of The Association Of American Law Schools, 1950), Karl Krastin

Florida Law Review

No abstract provided.

Lessons Learned: A Case Study On The Creation Of An Online Phd Program, Ashley H. Tharpe

FDLA Journal

This case study delves into the development of an online PhD program in Health Sciences at Liberty University, highlighting valuable lessons learned and offering insights for effective program creation and implementation. The study addresses the complexities of curriculum development, the importance of comprehensive communication, the role of faculty mentorship, and the significance of fostering a sense of community in online education. Drawing on the experiences of program developers, faculty members, and students, this research sheds light on strategies for aligning course content with program goals, embracing imperfection in program design, and cultivating a vibrant online learning community. Additionally, the study …

Evolution Of Global Anatomy Research Through Covid-19: A Post-Pandemic Evaluation, Harsh Chheda, Santanu De

FDLA Journal

The Coronavirus Infectious Disease 2019 (COVID-19) struck the world with a deep impact on many countries' healthcare systems. More specifically, anatomical research experienced a drastic setback due to the limitations and restrictions placed upon them by the medical community. This study aims to review how COVID-19 guidelines presented conflict regarding the retrieval and use of cadavers, as well as how anatomy research was modified on a global scale owing to the pandemic. The COVID-19 outbreak called for several changes upon each significant anatomical research technique, ranging from cadaver dissection to diagnostic imaging. Given the remarkable shift in medical research guidelines …

Global Implications Of Covid-19 On Education And Research In Healthcare And Stem: Analysis Of Case Studies, Yumna Indorewala, Santanu De

FDLA Journal

The zoonotic novel coronavirus, SARS-CoV-2 posed major threats to the world’s economy, social development, and public health. Education and research in STEM and healthcare were no exceptions to the global implications of the Coronavirus Infectious Disease 2019 (COVID-19) pandemic. Social distancing guidelines were initially recommended to refrain from in-person operations for preventing the transmission of the virus. As academic organizations implemented multifarious methods to effectively transition to remote/online or hybrid educational formats, COVID-19 necessitated designing, adopting, as well as evaluating the efficacy of new instructional approaches, especially for disciplines that require active, experiential learning such as healthcare and STEM. These …

Biphasic Burrowing In Atlantic Hagfish (Myxine Limosa), Douglas S. Fudge, Joshua Lee, Kennedy Guillen, Cassandra M. Donatelli, Andrew Lowe, Luke Arnold, Keolani Kahale-Lua, Christian Quinteros, Peter Ly, Larissa Atkins, Noah Bressman, Charlene L. Mccord

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Myxine limosa is a burrowing species of hagfish that occurs in the western North Atlantic in areas with muddy substrate and at depths generally greater than 100 meters. Burrowing of M. limosa has been observed from submersibles, but little is known about the behavior of these animals within the substrate or the biomechanical mechanisms involved. Here, we investigated burrowing in M. limosa by observing individuals as they burrowed through transparent gelatin. A photoelastic setup using crossed polarizers allowed us to visualize stress development in the gelatin as the hagfish moved through it. We found that M. limosa created U-shaped burrows …

Hardware Acceleration Of Numerical Methods For Solving Ordinary Differential Equations, Soham Bhattacharya

Theses and Dissertations

Along with the advancement in technology, the role of hardware accelerators is increasing consistently, delivering advancements in scientific simulations and data analysis in scientific computing, signal processing tasks in communication systems, matrix operations, and neural network computations in artificial intelligence and machine learning models. On the other hand, several high-speed computer applications in this era of high-performance computing often depend on ordinary differential equations (ODEs); however, their nonlinear nature can present a challenge to obtaining analytic solutions. Consequently, numerical approaches prove effective in delivering only approximate solutions to these equations. This research discusses the implementation of a customized hardware accelerator …

Educators’ Perceptions Of The Implementation And Influence Of The Middle School Concept, Carri Burns

Walden Dissertations and Doctoral Studies

The problem addressed in this study was that teachers struggle to apply the middle school concept in three middle schools in the U.S. region of New England. This study was a qualitative investigation of teachers’ perceptions of the middle school concept and their needs for additional support to effectively apply it. The conceptual framework for this study was the middle school concept itself, which theorizes five essential attributes of effective middle schools: responsive, challenging, empowering, equitable, and engaging. The research questions were about teachers’ perceptions of their knowledge about and application of the middle school concept, the extent to which …

Exploration Of Final-Year Teacher Preparation Through The Lens Of Emotional Intelligence, Dorina Sackman-Ebuwa

Walden Dissertations and Doctoral Studies

Although research has suggested that emotional intelligence (EI) is an important factor in career and life success, teacher preparation programs often fail to include topics for developing EI. The college of education that participated in this study sought to explore EI development in its teacher preparation program. This multimodal descriptive qualitative study explored the EI experiences of final-year preservice teachers to determine the extent to which their teacher preparation program developed EI from the participants’ perspectives. The conceptual framework combined transformative emotional intelligence (TEI), the emotional learning system (ELS), and an EI self-assessment of TEI skills. A normed EI profile …

Childhood Trauma, Attachment, And Adult Leadership Styles, Joshua S. Duncan

Walden Dissertations and Doctoral Studies

Past researchers have shown relationships between leadership styles and attachment and that secure attachment is positively related to transformational leadership. However, there is a gap in the research on how childhood trauma and attachment security relate to leadership styles in adults. The purpose of this quantitative study was to examine how childhood trauma and attachment security relate to transactional, transformational, and laissez-faire leadership styles. Social cognitive theory and attachment theory grounded this study. In this quantitative study, the specific research design was comparative. SurveyMonkey was used to gather data from supervisor/manager leaders who were recruited on social media (N = …

The Intersection Of Disability, Gender, And Sexual Violence Perceptions In Juror Decision-Making, Melissa West

Walden Dissertations and Doctoral Studies

Sexual violence is a societal epidemic and disproportionally affects disabled women. Disabled women belong to an intersectional identity that also experiences more negative attitudes, stigma, stereotypes, and discrimination than nondisabled individuals. In sexual assault cases, juror biases may translate into more detrimental attitudes toward disabled victims. The purpose of this study was to examine how these attitudes may impact outcomes in sexual assault trials when comparing disabled and nondisabled female victims. This study was conducted through the lens of just-world theory and feminist disability theory using a quantitative, experimental jury simulation design. The 304 participants in an online survey designed …

Factors Influencing Purpose In Life In Middle-Aged And Older Adults, Kelly Bergstrom

Dissertations

Purpose in life is an important dimension of psychological well-being with associated health benefits. The current study used a path analysis model to examine how perceived cognitive control, health locus of control- self, engagement in physical activity, and engagement in challenging cognitive activities relate to purpose in life longitudinally. Multimorbidity status was also examined as a potential moderator of paths in the model. Participants were middle-aged and older adults who participated in the Midlife in the United States (MIDUS) longitudinal national study (n = 2,481). After controlling for relevant covariates, engagement in physical activity, engagement in challenging cognitive activities, …

The Ecosystem As Super-Organ/Ism, Revisited: Scaling Hydraulics To Forests Under Climate Change, Jeffrey D. Wood, Matteo Detto, Marvin Browne, Nathan J.B. Kraft, Alexandra G. Konings, Joshua B. Fisher, Gregory R. Quetin, Anna T. Trugman, Troy S. Magney, Camila D. Medeiros, Nidhi Vinod, Thomas N. Buckley, Lawren Sack

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Classic debates in community ecology focused on the complexities of considering an ecosystem as a super-organ or organism. New consideration of such perspectives could clarify mechanisms underlying the dynamics of forest carbon dioxide (CO₂) uptake and water vapor loss, important for predicting and managing the future of Earth's ecosystems and climate system. Here, we provide a rubric for considering ecosystem traits as aggregated, systemic, or emergent, i.e., representing the ecosystem as an aggregate of its individuals, or as a metaphorical or literal super-organ or organism. We review recent approaches to scaling-up plant water relations (hydraulics) concepts developed for …

Evaluation Of Major Physical And Mechanical Properties Of Trembling Aspen Lumber, Dawei Wang, Mengyuan Zhang, Meng Gong, Ying-Hei Chui

Aspen Bibliography

Trembling aspen (Populus tremuloides) is one of the major species within Populus, a predominant genus of hardwoods in North America. However, its utilization has been limited to pulp and paper or wood-based composite boards. This study aimed at evaluating the major physical and mechanical properties of trembling aspen lumber, with an ultimate objective of using this species to produce engineered wood products (EWPs). The testing materials consisted of 2 × 4 (38 mm × 89 mm) trembling aspen lumber pieces in lengths of 8, 10, and 12 feet (2.44, 3.05, and 3.66 m) with two visual grades, …

Unveiling The Effectiveness Of Antimicrobial Bpj Polymer Coatings In Enhancing Microbial Resistance, Sonali Gupta, Yashoda Malgar Puttaiahgowda, Ananda Kulal

Open Access archive

This article introduces a novel method for combating microbial infections in textiles using advanced polymer coatings, particularly emphasizing formulations incorporating piperazine. We outline the synthesis and characterization of a copolymer consisting of piperazine, jeffamine, and bisphenol-a-diglycidyl ether, engineered to demonstrate robust antibacterial properties. Through rigorous testing, encompassing assessments of structural integrity and thermal resilience, we highlight the remarkable efficacy of this polymer in preventing microbial colonization. Notably, our antimicrobial studies reveal the coating's substantial resistance against various microbiological challenges, with significant zones of inhibition (ZOI) observed against Escherichia coli (14.6 mm), Staphylococcus aureus (9 mm), Mycobacterium smegmatis (10 mm) and …

The Effectiveness Of Pressure Injury Prevention Through Educational Intervention Within The Long-Term Care Setting, Ashley Nunley

Doctor of Nursing Practice Projects

The prevalence of pressure injuries, particularly in older adults, continues to be disturbingly high, especially in those suffering from chronic diseases and frailty, bringing a higher awareness of applied preventive and therapeutic measures (Jaul et al., 2019). In long-term care nurses, will an educational, evidenced-based pressure ulcer prevention program impact health outcomes by increasing nurses’ knowledge, compared to no intervention over four weeks? The Pieper-Zulkowski Pressure Ulcer/Injuries Knowledge Test was used to evaluate the nurse's pressure injury knowledge pre- and post-educational intervention. The paired t-test was used to analyze the pre-and post-test data from the educational intervention. This DNP project …

Fabrication Of Metallo-Pharmaceutical Composite Hydrogel Composed Of Curcumin-Loaded Cmc-Na/Sodium Alginate/Pdcl2: Optimization, Antimicrobial Activity, And Cancer Cell Mortality In Vitro Assessment, Samar A. Salim, Elbadawy A Kamoun, Mariam Emam, Shahira H El-Moslamy, Ayman Elsawaf, Amal Nassar, Nehal Eldeeb

Nanotechnology Research Centre

This work explores the fabrication route of curcumin-loaded carboxymethyl cellulose sodium/sodium alginate/palladium chloride (CMC-Na/SA/PdCl2) composite hydrogel as localized delivery system for cancer treatment. Herein, an approach was established for improving encapsulation capacity and exploiting therapeutic efficiency. CMC-Na/SA/PdCl2 composite hydrogels were prepared by gelation method using ionic crosslinker (PdCl2). Results proved that the optimized formula (CMC-Na:SA (4:1)/PdCl2 0.4%) recorded a gel fraction of 91% with a swelling ratio ~ 3000% after 96 h, while XRD analysis exhibited no remarks of sharp peaks which confirmed an amorphous phase of tested hydrogel. TGA results showed that this recipe is thermostable compared to other …