Digital Commons Network^™

Antidepressant Effect Of Enzymatic Porcine Placenta Hydrolysate In Repeated Immobilization Stress-Induced Ovariectomized Female Mice, Minsook Ye, Sharon Nguyen, Min Ju Kim, Jee Sun Hwang, Gun Won Bae, Keun-Hang Susan Yang, Insop Shim

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

When postmenopausal women are under stress conditions, this exacerbates mood disorders and issues with neuroimmune systems. The porcine placenta is known to relieve menopausal depression in clinical trials, but its underlying mechanisms for depression and anti-inflammatory functions remain poorly defined. The present study was designed to examine the anti-inflammatory effects of enzymatic porcine placenta hydrolysate (EPPH) on LPS-induced levels of nitric oxide (NO), prostaglandin E2 (PGE2), corticosterone (CORT), and pro-inflammatory cytokine interleukin-1 beta (IL-1β) in RAW 264.7 macrophage cells. In addition, the neurite outgrowth of PC12 cells was evaluated to examine the effects of EPPH on neurite growth. To mimic …

“Safer To Plant Corn And Beans”? Navigating The Challenges And Opportunities Of Agricultural Diversification In The U.S. Corn Belt, Rebecca Traldi, Lauren Asprooth, Emily M. Usher, Kristin Floress, J. Gordon Arbuckle, Megan Baskerville, Sarah P. Church, Ken Genskow, Seth Harden, Elizabeth Maynard, Aaron Thompson, Ariana P. Torres, Linda Stalker Prokopy

Department of Horticulture & Landscape Architecture Faculty Publications

Agricultural diversification in the Midwestern Corn Belt has the potential to improve socioeconomic and environmental outcomes by buffering farmers from environmental and economic shocks and improving soil, water, and air quality. However, complex barriers related to agricultural markets, individual behavior, social norms, and government policy constrain diversification in this region. This study examines farmer perspectives regarding the challenges and opportunities for both corn and soybean production and agricultural diversification strategies. We analyze data from 20 focus groups with 100 participants conducted in Indiana, Illinois, and Iowa through a combined inductive and deductive approach, drawing upon interpretive grounded theory. Our results …

Structural And Biophysical Insights Into Targeting Of Claudin-4 By A Synthetic Antibody Fragment, Satchal K. Erramilli, Pawel K. Dominik, Chinemerem P. Ogbu, Anthony A. Kossiakoff, Alex J. J.

Department of Biochemistry: Faculty Publications

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. As the backbone of tight junction structure and function, claudins are attractive targets for modulating tissue permeability to deliver drugs or treat disease. However, structures of claudins are limited due to their small sizes and physicochemical properties—these traits also make therapy development a challenge. Here we report the development of a synthetic antibody fragment (sFab) that binds human claudin-4 and the determination of a high-resolution structure of it bound to claudin-4/enterotoxin complexes …

Relationship Between Health Education Systems Incorporated Admission Assessment Scores And Student Completion Of A Baccalaureate Nursing Program, Colleen Peace

Walden Dissertations and Doctoral Studies

Math, reading, and vocabulary comprehension are important skills needed to ensure nursing students have a solid foundation on which to develop critical thinking skills to complete a nursing program. One of the common preadmission standardized tests is the Health Education Systems Incorporated Admission Assessment (HESI A2), but little research had been conducted to determine whether there is a correlation between the score on the HESI A2 and readiness to master nursing courses at the bachelor of science in nursing (BSN) level and completion of the BSN program. The purposes of this quantitative retrospective study, guided by Jeffreys’s nursing undergraduate retention …

Childhood Trauma, Attachment, And Adult Leadership Styles, Joshua S. Duncan

Walden Dissertations and Doctoral Studies

Past researchers have shown relationships between leadership styles and attachment and that secure attachment is positively related to transformational leadership. However, there is a gap in the research on how childhood trauma and attachment security relate to leadership styles in adults. The purpose of this quantitative study was to examine how childhood trauma and attachment security relate to transactional, transformational, and laissez-faire leadership styles. Social cognitive theory and attachment theory grounded this study. In this quantitative study, the specific research design was comparative. SurveyMonkey was used to gather data from supervisor/manager leaders who were recruited on social media (N = …

Relationship Between Health Education Systems Incorporated Admission Assessment Scores And Student Completion Of A Baccalaureate Nursing Program, Colleen Peace

Walden Dissertations and Doctoral Studies

Math, reading, and vocabulary comprehension are important skills needed to ensure nursing students have a solid foundation on which to develop critical thinking skills to complete a nursing program. One of the common preadmission standardized tests is the Health Education Systems Incorporated Admission Assessment (HESI A2), but little research had been conducted to determine whether there is a correlation between the score on the HESI A2 and readiness to master nursing courses at the bachelor of science in nursing (BSN) level and completion of the BSN program. The purposes of this quantitative retrospective study, guided by Jeffreys’s nursing undergraduate retention …

Exploration Of Final-Year Teacher Preparation Through The Lens Of Emotional Intelligence, Dorina Sackman-Ebuwa

Walden Dissertations and Doctoral Studies

Although research has suggested that emotional intelligence (EI) is an important factor in career and life success, teacher preparation programs often fail to include topics for developing EI. The college of education that participated in this study sought to explore EI development in its teacher preparation program. This multimodal descriptive qualitative study explored the EI experiences of final-year preservice teachers to determine the extent to which their teacher preparation program developed EI from the participants’ perspectives. The conceptual framework combined transformative emotional intelligence (TEI), the emotional learning system (ELS), and an EI self-assessment of TEI skills. A normed EI profile …

The Intersection Of Disability, Gender, And Sexual Violence Perceptions In Juror Decision-Making, Melissa West

Walden Dissertations and Doctoral Studies

Sexual violence is a societal epidemic and disproportionally affects disabled women. Disabled women belong to an intersectional identity that also experiences more negative attitudes, stigma, stereotypes, and discrimination than nondisabled individuals. In sexual assault cases, juror biases may translate into more detrimental attitudes toward disabled victims. The purpose of this study was to examine how these attitudes may impact outcomes in sexual assault trials when comparing disabled and nondisabled female victims. This study was conducted through the lens of just-world theory and feminist disability theory using a quantitative, experimental jury simulation design. The 304 participants in an online survey designed …

Educators’ Perceptions Of The Implementation And Influence Of The Middle School Concept, Carri Burns

Walden Dissertations and Doctoral Studies

The problem addressed in this study was that teachers struggle to apply the middle school concept in three middle schools in the U.S. region of New England. This study was a qualitative investigation of teachers’ perceptions of the middle school concept and their needs for additional support to effectively apply it. The conceptual framework for this study was the middle school concept itself, which theorizes five essential attributes of effective middle schools: responsive, challenging, empowering, equitable, and engaging. The research questions were about teachers’ perceptions of their knowledge about and application of the middle school concept, the extent to which …

Sting Agonist 8803 Reprograms The Immune Microenvironment And Increases Survival In Preclinical Models Of Glioblastoma, Hinda Najem, Spencer T Lea, Shashwat Tripathi, Lisa Hurley, Chao-Hsien Chen, Ivana William, Moloud Sooreshjani, Michelle Bowie, Genevieve Hartley, Corey Dussold, Sebastian Pacheco, Crismita Dmello, Catalina Lee-Chang, Kathleen Mccortney, Alicia Steffens, Jordain Walshon, Martina Ott, Jun Wei, Anantha Marisetty, Irina Balyasnikova, Roger Stupp, Rimas V Lukas, Jian Hu, Charles David James, Craig M Horbinski, Maciej S Lesniak, David M Ashley, Waldemar Priebe, Leonidas C Platanias, Michael A Curran, Amy B Heimberger

Faculty, Staff and Student Publications

STING agonists can reprogram the tumor microenvironment to induce immunological clearance within the central nervous system. Using multiplexed sequential immunofluorescence (SeqIF) and the Ivy Glioblastoma Atlas, STING expression was found in myeloid populations and in the perivascular space. The STING agonist 8803 increased median survival in multiple preclinical models of glioblastoma, including QPP8, an immune checkpoint blockade-resistant model, where 100% of mice were cured. Ex vivo flow cytometry profiling during the therapeutic window demonstrated increases in myeloid tumor trafficking and activation, alongside enhancement of CD8+ T cell and NK effector responses. Treatment with 8803 reprogrammed microglia to express costimulatory CD80/CD86 …

Design And Characterization Of 3d-Printed Hydrogel Lattices With Consistent Structural And Mechanical Anisotropy, Daniel Yoon

McKelvey School of Engineering Graduate Student Theses & Dissertations

This thesis explores the use of 3D printing to fabricate soft hydrogel lattice structures with consistent properties, including varying levels of mechanical anisotropy. Magnetic resonance elastography (MRE) is a non-invasive imaging tool to estimate and characterize the mechanical properties of tissue. MRE “phantoms” (physical surrogate models) have been used to assess how well MRE can measure and estimate mechanical properties. While gel phantoms generally simulate brain tissue, almost all have been isotropic. Prior studies have shown that white matter brain tissue is structurally and mechanically anisotropic. To validate anisotropic MRE, phantoms with controllable, reproducible anisotropic material properties are needed. This …

Investigation Of Self-Assembly Of Hen Egg White Lysozyme Fibrils, Nabila Bushra

USF Tampa Graduate Theses and Dissertations

Amyloid proteins are a large group of proteins that tend to aggregate and form insoluble fibrils with a characteristic cross-β sheet structure. Intriguingly, this process of “amyloid formation” has been simultaneously linked to devastating human diseases called amyloidoses, ranging from Alzheimer’s Disease, cardiac amyloidoses to type II diabetes, as well as functional biological responses such as hormone storage and sperm quality control. Amyloid fibrils have also attracted significant attention for their unique mechanical, chemical, and optical characteristics, establishing them as promising tough biomaterials. The urgent need to find treatments for Alzheimer’s disease, and the demand for safe and renewable biomaterials …

Detection Of Wintertime Green Vegetated Cover Using Object-Based Classification With Open-Source Remote Sensing And Geospatial Technologies, Jae Sung Kim, Sara P. Syswerda, Sigrid D. P. Smith

Michigan Tech Publications

It is important to detect wintertime green vegetated cover (WGVC), since it includes cover crop information, which is one of the most important agricultural best management practices used today. Related to this, cover crop area, which is part of WGVC, has been estimated using survey methods traditionally, but remote sensing can be used as a more time- and cost-effective assessment. Previously developed pixel-based methods to assess cover crops using remote sensing can have a salt-and-pepper effect, which lowers classification accuracy. Therefore, object-based classification was applied to estimate the spatial distribution of WGVC across the entire state of Delaware. To reduce …

Tracing The Digital Plant Humanities: Narratives Of Botanical Life And Human-Flora Relations, Paul Longley Arthur, John Charles Ryan

Research outputs 2022 to 2026

In this paper, we trace the emergence of what we term the digital plant humanities (DPH) as an evolution of burgeoning botanical interest among environmental and digital humanists. We argue that DPH coalesces the theoretical and methodological frameworks of the three research areas of plant humanities, environmental humanities, and digital humanities. After conceptualising DPH, we analyse three projects representative of the emergent field—the Native American Ethnobotany Database; Herbaria 3.0; and Microcosms: A Homage to Sacred Plants of America—while referring to a broader range of formative projects including the Plant Humanities Lab.

Association Of Basal Forebrain Atrophy With Cognitive Decline In Early Alzheimer Disease, Ying Xia, Vincent Dore, Jurgen Fripp, Pierrick Bourgeat, Simon M. Laws, Christopher J. Fowler, Stephanie R. Rainey-Smith, Ralph N. Martins, Christopher Rowe, Colin L. Masters, Elizabeth J. Coulson, Paul Maruff

Research outputs 2022 to 2026

Background and Objectives: In early Alzheimer disease (AD), β-amyloid (Aβ) deposition is associated with volume loss in the basal forebrain (BF) and cognitive decline. However, the extent to which Aβ-related BF atrophy manifests as cognitive decline is not understood. This study sought to characterize the relationship between BF atrophy and the decline in memory and attention in patients with early AD.

Methods: Participants from the Australian Imaging, Biomarkers and Lifestyle (AIBL) study who completed Aβ-PET imaging and repeated MRI and cognitive assessments were included. At baseline, participants were classified based on their clinical dementia stage and Aβ status, yielding groups …

A Country-Level Primary-Final-Useful (Cl-Pfu) Energy And Exergy Database: Overview Of Its Construction And 1971–2020 World-Level Efficiency Results, Paul E. Brockway, Matthew Kuperus Heun, Zeke Marshall, Emmanuel Aramendia, Paul Steenwyk, Thomas Relph, Michelle Widjanarko, Jeonghoo (James) Kim, Anjana Sainju, Julian Irtube

University Faculty Publications and Creative Works

Societal exergy analysis examines the flows of energy and exergy through societies, from primary (e.g. oil) to final (e.g. gasoline) to useful (e.g. propulsion) energy stages. By extending the study of energy to the useful stage, new insights into the under-represented role of energy in economic growth have been made. However, currently (a) country coverage is patchy and incomplete, (b) available data are based on varying methods and assumptions including efficiencies based on economic rather than engineering data, and (c) datasets are constructed using piecemeal computational approaches. To address these gaps, we construct a country-level primary-final-useful (CL-PFU) energy and exergy …

Business Innovation As A Force For Good: From Doing Less Harm To Positive Impact Type 1 And Type 2, Chris Laszlo, David Cooperrider, Ronald Fry

Faculty Scholarship

Commitments of “getting to zero” or becoming a regenerative company are raising investor, customer, and employee expectations at a time when businesses are struggling just to reduce negative impacts. Executives are increasingly caught between wanting to build a better world and the reality of managing value-add activities that continue to harm people and the environment. Businesses need to distinguish between three types of innovation impacts to maintain their credibility and legitimacy. The first is doing less harm, where the goal is to minimize social wrongs and reduce ecological footprints. The second is incremental positive impact, where the goal is to …

A Country-Level Primary-Final-Useful (Cl-Pfu) Energy And Exergy Database: Overview Of Its Construction And 1971–2020 World-Level Efficiency Results, Paul E. Brockway, Matthew Kuperus Heun, Zeke Marshall, Emmanuel Aramendia, Paul Steenwyk, Thomas Relph, Michelle Widjanarko, Jeonghoo (James) Kim, Anjana Sainju, Julian Irtube

University Faculty Publications and Creative Works

Societal exergy analysis examines the flows of energy and exergy through societies, from primary (e.g. oil) to final (e.g. gasoline) to useful (e.g. propulsion) energy stages. By extending the study of energy to the useful stage, new insights into the under-represented role of energy in economic growth have been made. However, currently (a) country coverage is patchy and incomplete, (b) available data are based on varying methods and assumptions including efficiencies based on economic rather than engineering data, and (c) datasets are constructed using piecemeal computational approaches. To address these gaps, we construct a country-level primary-final-useful (CL-PFU) energy and exergy …

Structural Characterization Of Ligand Binding And Ph-Specific Enzymatic Activity Of Mouse Acidic Mammalian Chitinase, Roberto Efraín Díaz, Andrew K Ecker, Galen J Correy, Pooja Asthana, Iris D Young, Bryan Faust, Michael C Thompson, Ian B Seiple, Steven Van Dyken, Richard M Locksley, James S Fraser

2020-Current year OA Pubs

Chitin is an abundant biopolymer and pathogen-associated molecular pattern that stimulates a host innate immune response. Mammals express chitin-binding and chitin-degrading proteins to remove chitin from the body. One of these proteins, Acidic Mammalian Chitinase (AMCase), is an enzyme known for its ability to function under acidic conditions in the stomach but is also active in tissues with more neutral pHs, such as the lung. Here, we used a combination of biochemical, structural, and computational modeling approaches to examine how the mouse homolog (mAMCase) can act in both acidic and neutral environments. We measured kinetic properties of mAMCase activity across …

Discrete Relationships Between Spatiotemporal Gait Characteristics And Domain-Specific Neuropsychological Performance In Midlife, Laura Morrison, Adam Dyer, Helena Dolphin, Isabelle Killane, Nollaig M. Bourke, Matthew Widdowson, Conor P. Woods, James Gibney, Richard B. Reilly, Sean Kennelly

Research Outputs: 2025-Present

Midlife risk factors such as type 2 diabetes mellitus (T2DM) confer a significantly increased risk of cognitive impairment in later life with executive function, memory, and attention domains often affected first. Spatiotemporal gait characteristics are emerging as important integrative biomarkers of neurocognitive function and of later dementia risk. We examined 24 spatiotemporal gait parameters across five domains of gait previously linked to cognitive function on usual-pace, maximal-pace, and cognitive dual-task gait conditions in 102 middle-aged adults with (57.5 ± 8.0 years; 40% female) and without (57.0 ± 8.3 years; 62.1% female) T2DM. Neurocognitive function was measured using a neuropsychological assessment …

Mitigating Toxic Metal Exposure Through Leafy Greens: A Comprehensive Review Contrasting Cadmium And Lead In Spinach, Angelia L. Seyfferth, Matthew A. Limmer, Benjamin R. Runkle, Rufus L. Chaney

Biological Sciences Faculty Publications and Presentations

Metals and metalloids (hereafter, metal(loid)s) in plant-based foods are a source of exposure to humans, but not all metal(loid)-food interactions are the same. Differences exist between metal(loid)s in terms of their behavior in soils and in how they are taken up by plants and stored in the edible plant tissue/food. Thus, there cannot be one consistent solution to reducing toxic metal(loid)s exposure to humans from foods. In addition, how metal(loid)s are absorbed, distributed, metabolized, and excreted by the human body differs based on both the metal(loid), other elements and nutrients in the food, and the nutritional status of the human. …

The Relationship Between Communicative And Social Deficits And Challenging Behavior In Angelman Syndrome, Cassie Chanel Feck

LSU Doctoral Dissertations

Angelman Syndrome (AS) is a low-incidence neurodevelopmental disorder characterized by deficits in speech, motor, and social development as well as related differences in challenging behavior, seizures, and sleep issues. AS has a known genetic etiology and research conducted with this population has overwhelmingly focused on exploring the genetic and biological underpinnings of the disorder (e.g., applied gene therapies). At present, far less research on supporting the social, communicative, and behavioral needs of this population and how these specific deficits contribute to the individual’s overall functioning. The goals of this study were to characterize the use of Augmentative and Alternative Communication …

Proceedings Of The Cuny Games Conference 10.0, Robert O. Duncan, Grace Axler-Diperte, Joe Bisz, Christina Boyle, Devorah Kletenik, Carolyn Stallard

Publications and Research

The ten-year anniversary (!) of the CUNY Games Conference combines workshops, idea exchanges, interactive participant presentations, playtesting, and playing tabletop games into a two-day hybrid event to promote and discuss game-based learning. The conference focuses on creative pedagogy, such as playful learning activities or games, that teachers can use in the classroom every day. Day 1 featured interactive presentations by attendees, informal idea exchange sessions, and workshops by the conference organizers. Day 2 featured select presentations and workshops, poster sessions, playtesting and game modding, and casual play of tabletop games.

Maldi Imaging Mass Spectrometry Visualizes The Distribution Of Antidepressant Duloxetine And Its Major Metabolites In Mouse Brain, Liver, Kidney, And Spleen Tissues, Saleh M Khalil, Xuan Qin, John M Hakenjos, Jian Wang, Zhaoyong Hu, Xinli Liu, Jin Wang, Mirjana Maletic-Savatic, Kevin R Mackenzie, Martin M Matzuk, Feng Li

Faculty, Staff and Student Publications

Imaging mass spectrometry (IMS) is a powerful tool for mapping the spatial distribution of unlabeled drugs and metabolites that may find application in assessing drug delivery, explaining drug efficacy, and identifying potential toxicity. This study focuses on determining the spatial distribution of the antidepressant duloxetine, which is widely prescribed despite common adverse effects (liver injury, constant headaches) whose mechanisms are not fully understood. We used high-resolution IMS with matrix-assisted laser desorption/ionization to examine the distribution of duloxetine and its major metabolites in four mouse organs where it may contribute to efficacy or toxicity: brain, liver, kidney, and spleen. In none …

The Ifitm5 Mutation In Osteogenesis Imperfecta Type V Is Associated With An Erk/Sox9-Dependent Osteoprogenitor Differentiation Defect, Ronit Marom, I-Wen Song, Emily C Busse, Megan E Washington, Ava S Berrier, Vittoria C Rossi, Laura Ortinau, Youngjae Jeong, Ming-Ming Jiang, Brian C Dawson, Mary Adeyeye, Carolina Leynes, Caressa D Lietman, Bridget M Stroup, Dominyka Batkovskyte, Mahim Jain, Yuqing Chen, Racel Cela, Alexis Castellon, Alyssa A Tran, Isabel Lorenzo, D Nicole Meyers, Shixia Huang, Alicia Turner, Vinitha Shenava, Maegen Wallace, Eric Orwoll, Dongsu Park, Catherine G Ambrose, Sandesh Cs Nagamani, Jason D Heaney, Brendan H Lee

Faculty, Staff and Students Publications

Osteogenesis imperfecta (OI) type V is the second most common form of OI, distinguished by hyperplastic callus formation and calcification of the interosseous membranes, in addition to the bone fragility. It is caused by a recurrent, dominant pathogenic variant (c.-14C>T) in interferon-induced transmembrane protein 5 (IFITM5). Here, we generated a conditional Rosa26-knockin mouse model to study the mechanistic consequences of the recurrent mutation. Expression of the mutant Ifitm5 in osteo-chondroprogenitor or chondrogenic cells resulted in low bone mass and growth retardation. Mutant limbs showed impaired endochondral ossification, cartilage overgrowth, and abnormal growth plate architecture. The cartilage phenotype correlates with …

Cancer-Associated Histone H3 N-Terminal Arginine Mutations Disrupt Prc2 Activity And Impair Differentiation, Benjamin A Nacev, Benjamin A Garcia, Et Al.

2020-Current year OA Pubs

Dysregulated epigenetic states are a hallmark of cancer and often arise from genetic alterations in epigenetic regulators. This includes missense mutations in histones, which, together with associated DNA, form nucleosome core particles. However, the oncogenic mechanisms of most histone mutations are unknown. Here, we demonstrate that cancer-associated histone mutations at arginines in the histone H3 N-terminal tail disrupt repressive chromatin domains, alter gene regulation, and dysregulate differentiation. We find that histone H3R2C and R26C mutants reduce transcriptionally repressive H3K27me3. While H3K27me3 depletion in cells expressing these mutants is exclusively observed on the minor fraction of histone tails harboring the mutations, …

What Factors Affect Empathy In People With Narcissism?, Ya Yang, Liza Oh

Pacific Journal of Health

This literature review analyzes factors that may affect how people with narcissism experience empathy. The articles that were analyzed in the literature review included those that discussed the relationship between narcissism and empathy. Using a systematic review, a total of 11 articles were chosen from PsycINFO through a three-stage screening process. The studies mainly revolved around perspective-taking, traits associated with narcissism, motivation, and gender. The participants included in the studies were mainly adults in the United States that had no, low, or high narcissism. The main finding was that factors that caused people with narcissism to view others as if …

Great Bay Estuary (Grb) Nerr Nutrient Metadata April 2023 Through December 2023, Thomas K. Gregory, Lara M. Martin

PREP Reports & Publications

No abstract provided.

Isolation Of Gametophytic Selection And Selective Embryo Abortion In Mimulus Guttatus, Desmond C. Willson

University Honors Theses

A lack of genomic studies examining gametophytic selection (GS) and selective embryo abortion (SEA)--which occur during the reproduction of angiosperms--leaves questions regarding the adaptive and evolutionary effects of these processes. Analyzing deviations from Mendelian segregation offers an avenue for identifying loci targeted by GS and SEA, and their contributions to purging of genetic load. However, other selective processes such as meiotic drive and cytonuclear interactions, as well as pollen and ovule abortion, can cause distortion. To distinguish the effects of GS and SEA from other causes of distortion, we will perform reciprocal crosses between highly homozygous and highly heterozygous individuals …

Compassionate Practice: A Review And Framework For Integrating Medical Humanities Into Pre-Medical And Medical Curricula, Megan Mccoy

University Honors Theses

The article presents the findings of a comprehensive narrative literature review aimed at addressing the question: What are the current frameworks of the medical humanities discipline in terms of purpose, methodology, curriculum, and student learning outcomes? Thematic analysis and qualitative coding highlighted key themes and areas of concern, including the purpose of medical humanities, methodology, current curriculum, and student outcomes. Recommendations are provided to address disagreements within the discipline, proposing a conceptual framework and definition for medical humanities, alongside advocating for a mixed-method approach as the primary methodology. Curriculum guidelines emphasize the importance of a quality integrated curriculum focusing on …