Investigating The Multiple Enzyme Complex In The Carotenoid Biosynthesis Pathway, Mercy Mormor

Electronic Theses and Dissertations

In nature, plant carotenoids are 40-carbon isoprenoids with polyene chains that may have double bonds. They are vital components of all photosynthetic organisms, playing several roles in the plant cell, including photosynthesis, pigmentation, phytohormone synthesis, signaling and photoprotection. In this study, we investigated protein-protein interactions among key enzymes involved in the carotenoid biosynthesis pathway using the Bimolecular Fluorescent Complementation (BiFC) assay. Our results revealed several interactions that suggest a coordinated regulation of enzymatic activity within this pathway, highlighting potential regulatory complexes that influence carotenoid production. The study also highlights the pivotal role of Zeta-Carotene Isomerase (Z-ISO) as a key regulatory …

Gut Passage Time In Small Mammals: Influences Of Small Mammal Traits, Digesta Phase, And Particle Morphology, Noah G. Hale

Electronic Theses and Dissertations

Dispersal is crucial in maintaining healthy ecosystems, but little is known about mechanisms influencing fungal spore dispersal by animals. We aimed to develop a more mechanistic understanding of the influence of fungal spore traits and small mammal traits on gut retention time. We fed three species of small mammals (shrew, mouse, vole), which differ in gut morphologies, digestive strategies, and activity a combination of markers including: liquid digesta phase (CrEDTA), small particle (fungal spores with diverse morphologies), and large particles (glitter and beads, representing plant cells and seeds, respectively). We found that hindgut fermenters selectively retained the liquid markers, spores …

Cattle Regenerative Grazing Effects On Bat Activity In Southern Appalachia, Seth Morelock

Electronic Theses and Dissertations

Livestock farming accounts for about 70% of agricultural activity, with continuous grazing as the dominant conventional practice. However, this method often degrades biodiversity and ecosystem health through overgrazing. Regenerative grazing practices offer a potential ecologically sustainable approach to cattle management. Insectivorous bats are known to use agroecosystems for foraging and roosting habitats, offering an opportunity to assess their response to regenerative grazing. Using passive acoustic monitors, we evaluated the response of bats to cattle within regenerative grazed farms in East Tennessee, USA. Overall bat activity declined when cattle entered and exited paddocks, except for the tri-colored bat (Perimyotis subflavus …

A Grounded Theory Investigation Of The Experiences Of Rehabilitation Administrators, Counselors, And Supervisors Providing Services To Women With Disabilities Experiencing Intimate Partner Violence, Dana Posey

Graduate Theses and Dissertations

Abstract There is a noticeable gap in the literature as it relates to how rehabilitation and disability service providers have worked with women with disabilities who have experienced intimate partner violence. To address the dearth of knowledge, a qualitative grounded theory study was conducted with 12 rehabilitation administrators, counselors, and supervisors regarding their experiences providing services to women with disabilities who have been in or are in an intimate partner violence relationship. Semi-structured interviews were administered, recorded, and transcribed verbatim in an ongoing manner throughout the process. As data was collected, it was analyzed using NVIVO 15, which is a …

Impact Of Three Professional Development Models (Online, Face-To-Face, And Hybrid) On Bhutanese Inservice Science Teachers' Conceptions Of Aspects Of Nature Of Science (Nos) With Reports Of Translation Of Nos Content Into Classroom Teaching Practices, Pabi Maya Das

Graduate Theses and Dissertations

Having a scientifically literate citizenry is an asset nationally and globally, and knowledge of the nature of science (NOS) is a crucial element in such literacy. Science teachers are a vital link if students are to understand key elements of the NOS, yet teachers themselves often lack knowledge of NOS and how to integrate NOS content into science lessons. Investigating the impact of a series of inservice professional development experiences using three distinct delivery modes (face-to-face, hybrid, online) for teachers in my home country of Bhutan is the overarching goal of the study reported here. The study used a mixed-methods …

Critical Communication Pedagogy At The End Of The World, Tyler S. Rife

Journal of Communication Pedagogy

This essay draws upon my experiences teaching the undergraduate course, “Communication at the End of the World,” to argue for an approach to critical communication pedagogy (CCP) contextualized by and reflexively responsive to planetary-scale ecological crises. I outline how five commitments have come to guide my classroom’s explorations through our topic of “end-times” communication: (1) Anthropocentrism and speciesism participate in the maintenance of social hierarchy; (2) environmental justice and social justice are intimately entangled projects; (3) ecological crisis is (re)made concrete, embodied, proximal, and implicative; (4) critique of language involves critique of humanism and humanist historicization; and, (5) ecologically just …

Comparative Phylogeography Of Three Widely Distributed Amazonian Poison Frogs, Sofia Del Milagro Granados Martinez

Theses

Amazonia has gone through extensive changes in its climates, geology, hydrology and drainage systems over the past 10 mya; these processes have been postulated as key drivers of diversification. Contrary to the trend where species once broadly distributed are subdivided into several species with restricted distributions, many taxa span the Amazon basin without noticeable genetic differentiation. In my thesis, I studied the phylogenetic and phylogeographic patterns of three widely distributed Amazonian poison frogs: Ameerega hahneli, Ameerega trivittata and Ranitomeya variabilis. I examined phylogenetic relationships based on two different types of genomic data—nuclear and mitochondrial—and I carried out population structure analyses. …

Microplastics And Other Plastic-Like Microdebris In Sediments From The Dart River And Estuary, Southwest England, Maia Killian, Andrew Turner

School of Geography, Earth and Environmental Sciences

Surficial sediment samples (n = 10) collected from a transect of a river-estuary (Dart) in southwest England have been analysed for geochemical characteristics, anthropogenic metals (Cr, Cu, Sn, Pb, Zn), and microplastics (∼50 μm–5 mm) and other microplastic-like debris. Results indicated the finest grains towards the lower river and upper estuary, a seaward increase in sedimentary Ca, and loss on ignition ranging from about 0.7 to 4 %. Anthropogenic metals were heterogeneously distributed throughout the catchment with maximum concentrations in the river or lower estuary. Microplastics were dominated by fibres, with concentrations ranging from 110 to 1800 kg−1 and composition …

Sarcoplasmic Reticulum-Mitochondria Microdomains: Hugging And Kissing In The Heart, Bong Sook Jhun, Jin O-Uchi, Brian Rhee, Ameneh Ahrari, Nathan Demichaelis, Kye-Im Jeon, David M. Booth, Shey-Shing Sheu

Center for Translational Medicine Faculty Papers

Endoplasmic reticulum (ER)-mitochondrial (ER-Mito) interface, termed mitochondrial-ER contacts (MERCs), plays significant roles in the maintenance of bioenergetics and basal cell functions via the exchange of lipids, Ca 2 þ , and reactive oxygen species (ROS) in various cell types/tissues. Genetic deletion of mitofusin 2 (Mfn2), one of the key components of ER-Mito tethering, in cardiomy- ocytes (CMs) in vivo revealed the importance of the microdomains between mitochondria and sarcoplasmic reticulum (SR), a dif- ferentiated form of ER in muscle cells, for maintaining normal mitochondrial Ca 2 þ (mtCa 2 þ ) handling and bioenergetics in the adult heart. However, key …

Factors Underlying A Latitudinal Gradient In The S/G Lignin Monomer Ratio In Natural Poplar Variants, Jin Zhang, Pradeep Kumar Prabhakar, Yen On Chan, Rachel A. Weber, Mengjun Shu, Ganesh Panzade, Connor J. Cooper, Russell B. Davidson, Jin-Gui Chen, Sara S. Jawdy, Jerry M. Parks, Breeanna R. Urbanowicz, Gerald A. Tuskan, Trupti Joshi, Richard A. Dixon, Wellington Muchero, Jaime Barros

Biomedical Sciences

The chemical composition of wood plays a pivotal role in the adaptability and structural integrity of trees. However, few studies have investigated the environmental factors that determine lignin composition and its biological significance in plants. Here, we examined the lignin syringyl-to-guaiacyl (S/G) ratio in members of a Populus trichocarpa population sourced from their native habitat and conducted a genome wide association study to identify genes linked to lignin formation. Our results revealed many significant associations, suggesting that lignin biosynthesis is a complex polygenic trait. Additionally, we found an increase in the S/G ratio from northern to southern geographic origin of …

Differential Inhibition Of Tumor Oxidative Metabolism While Sparing T Cells Through A Novel Genetic Approach, Jean Schneider, Michael Curran Phd, Priyamvada Jayaprakash Phd, Krithikaa Rajkumar Bhanu

Students Summer in Oncology at Anderson Research (SOAR) 2025

In pursuit of identifying new potential targets for immunotherapy, there has been renewed focus on Oxidative Phosphorylation (OxPhos) metabolism that cancers rely on for growth and, to some degree, immune suppression. Previous research has shown selective deletion of the mitochondrial Complex I (CI) subunits Ndufs4 and Ndufs6 increases the efficacy of immunotherapy through induction of genes related to MHC class-I expression leading to improved tumor antigen presentation. Based on these studies, we hypothesize that selectively targeting CI subunits differentially expressed in tumor cells versus immune effector cells will significantly hinder tumor cell viability and growth without compromising anti-tumor immunity.

Embryos On Ice: Issues With Estate Law And Assisted Reproduction In A Post-Dobbs World, Nicole Pomerantz

University of Florida Journal of Law & Public Policy

Will you have to share your property interests with an embryo in a petri dish? This Note explores the complex legal landscape surrounding the estate interests of cryopreserved embryos in the wake of the Supreme Court’s decision in Dobbs v. Jackson Women’s Health Organization. The piece delves into the implications of recognizing embryos as legal persons with rights, particularly in the context of estate law, where the classification of embryos can significantly impact property distribution, class-closing rules, and the application of the Rule Against Perpetuities. By examining case law, statutes, and the potential consequences of states adopting the Dobbs …

"No One Leaves For Free": A Media Analysis Of The Lived Experiences And Cumulative Impacts Of The Flint Water Crisis., Jordan Leigh Gatewood

Electronic Theses and Dissertations

In April 2014, the city of Flint, Michigan changed their municipal water source from Lake Huron to the Flint River. The effects of this change have been felt far and wide as news broke across the nation that this water was contaminated with lead and residents of Flint were getting sick. This research analyzes 170 news articles from three local and three national news outlets over a 10-year span, 2014 to 2024, to determine how community voice is presented in news media to portray the lived experiences and cumulative impacts of the Flint Water Crisis. Applying a Critical Environmental Justice …

Rheumatic Heart Disease In Primary Aged Children In Samoa, Kallysta Lindquist

Undergraduate Honors Theses

Rheumatic heart disease (RHD) is a condition where the heart valves are permanently damaged by an autoimmune response to chronic strep infections. RHD remains extremely prevalent in Oceania - specifically Samoa. However, despite its prevalence, RHD is underexplored in Samoa and there are many disparities for medical care to treat RHD in Samoa. This research shifts the focus of RHD to early detection in children (5-18 years old) and provides critical insights into RHD and the geographic difference in RHD prevalence in Samoa. Our dataset was collected between 2013-2019 from the Rheumatic Relief program done by Brigham Young University. Data …

Guide For Teaching Faculty 2025-2026, Coastal Carolina University

Guide for Teaching Faculty

The intention of the Guide for Teaching Faculty is to ensure that all faculty members have the requisite knowledge about University policies, procedures, and services to effectively carry out their instructional assignments.

Oyster Shell Recycling Efforts In Horry County, Corabella Dieguez

Goal 14: Life Below Water

No abstract provided.

Reducing Single-Use Plastic In Georgetown County, Alex Gaspar

Goal 12: Responsible Consumption and Production

No abstract provided.

Sccosmix: A Mixed-Effects Framework For Differential Coexpression And Transcriptional Interactions Modeling In Single-Cell Rna-Seq, Anderson Bussing, Giampiero Marra, Daping Fan, Russell Shinohara, Danni Tu, Yen-Yi Ho

Faculty Publications

Advancements in single-cell RNA-sequencing (scRNA-seq) technologies generate a wealth of gene expression data that provide exciting opportunities for studying gene-gene interactions systematically at individual cell resolution. Genetic interactions within a cell are tightly regulated and often highly dynamic in response to internal cellular signals and external stimuli. Evidence of these dynamic interactions can often be observed in scRNA-seq data by examining conditional co-expression changes. Existing approaches for studying these dynamic interaction changes in scRNA-seq data do not address the multi-subject hierarchical design commonly considered in single-cell experiments. In this paper, we propose a Mixed-effects framework for differential Coexpression and transcriptional …

Assessment Of A Muscadine Grape (Vitis Rotundifolia) Mapping Population For Tolerance To Pierce’S Disease Pathogen Xylella Fastidiosa And Resistance To Black Rot Disease Pathogen Guignardia Bidwellii, Anushka Tennakoon

Master's Theses

Pierce’s disease (PD) and black rot disease cause severe economic losses for grape production under humid and warm conditions. Identifying resistance genes is crucial for controlling these disease by developing resistant cultivars. Muscadine grape (Vitis rotundifolia) cultivar ‘Pam’ has been identified to possess a high level of resistance to diseases. To elucidate the genetic basis of the resistance to the causal pathogens Xylella fastidiosa and Guignardia bidwellii in this cultivar, parents and 141 F1 individuals developed from open pollinated ‘Pam’ were evaluated for two seasons under natural disease pressure. The mapping population was genotyped using genotyping-by-sequencing (GBS) and …

The Effects Of Temperature And Cardiotonic Steroids On Cardiac Function And Metabolism In Snakes, Hannah Wilson

All Graduate Theses and Dissertations, Fall 2023 to Present

The function of the heart provides a window into the physiology of vertebrates and a means of evaluating their response to the stresses imposed by diverse activities. I developed a miniaturized instrument to record electrocardiograms (ECGs), using open-source Arduino technology, that a snake can wear as a backpack. The backpack can also be used when the snake is in a metabolic chamber, to record the metabolic rate and ECG simultaneously. I first used the ECG backpack to record the physiology of snakes under different temperature conditions, to verify that the system worked as expected. the backpack was then used on …

Msw Portfolio, Krystal F. Youngs

Graduate Student Portfolios, Professional Papers, and Capstone Projects

No abstract provided.

Projecting Echoes: Valeria Luiselli's Writing On Immigration, Jacob C. Cisneros

Electronic Theses, Projects, and Dissertations

The growing concerns of immigration has been a fraught topic in both U.S. society and the American political landscape. Certain political narratives are formed which fuel the debate for and against immigration. This coincides with how immigrants are treated and how legal processes change throughout each term of presidency within the United States. This has sparked outrage from both advocates and adversaries of immigrants coming into the United States. Papers, speeches, news articles, and more have been created from both sides of the argument concerning how to handle the immigration crisis along with the question of how immigrants and asylum …

Association Between Social Participation And Nonmotor Symptoms Of Parkinson's Disease, Zackary B. Zanotelli

Electronic Theses, Projects, and Dissertations

Research has shown that social participation can aid in slowing cognitive decline and is associated with cognitive functioning in older adults. Being that Parkinson’s Disease (PD) is a neurodegenerative disorder primarily known for its motor and cognitive symptoms, it is worth evaluating social participation in patients with Parkinson’s Disease. The aim of this project is to determine whether social participation is associated with more favorable trajectories of neuropsychiatric symptoms among patients afflicted with Parkinson’s Disease. More specifically, this project will seek to examine how specific social activities may be uniquely associated with cognitive decline.

Participants (N = 8,389) who were …

Medical Surveillance In Custodial Jsas: Preventing Bloodborne Pathogen Exposure, Kiara Gadea

Electronic Theses, Projects, and Dissertations

Custodians play an important role in maintaining our surroundings safe and sanitary. However, in doing so, they are their exposure to health hazards that put them at risk, yet these exposures are often overlooked. One major risk they face is contact with bloodborne pathogens, which put custodial staff at risk for infections such as hepatitis B. While Job Safety Analyses (JSA’s) outline various protective measures, many fail to incorporate medical surveillance as part of their risk management program. Overlooking this important detail leaves custodians vulnerable to health risks that can be prevented.

Adding a medical surveillance section to custodial JSA’s, …

The Use Of Sludge Content For Effective Recall Of Information, Rahaf G. Fares

Electronic Theses, Projects, and Dissertations

In an age where social media dominates daily consumption, the impact of engaging multi-modal media, specifically “sludge content,” on attention and memory has garnered significant interest. Sludge content, which generally combines unrelated visual gameplay with narrated audio and subtitles, has emerged as a popular form of online entertainment. This study explored whether this type of content enhances information recall by capturing users’ attention, a claim bolstered by theories of optimal arousal, non-pharmacological interventions such as fidget toys, and audiovisual redundancy. Using a within-subjects experimental design, the study investigated the effects of sludge content on effective recall through three conditions: sludge …

Temperature Effects On Fruit Doubling And Bloom Prediction In Tart Cherry, Josette M. Moon

All Graduate Theses and Dissertations, Fall 2023 to Present

Tart cherry (Prunus cerasus) is a key horticultural crop in Utah. Extreme summer heat and cold weather in late spring are challenges to tart cherry production causing economic losses. These problematic weather conditions are influenced by the local landscape and are exacerbated by changing climate. This research explores (1) the impact of summer heat stress on fruit deformities, and (2) the accuracy of temperature-based predictions of spring bud development. High temperatures and water stress after harvest are known to cause a deformity known as twin or double fruits, resulting in unmarketable fruit. This disorder has been studied in …

Lifetime Stressor Exposure, Race And Ethnicity, And Habitual Stress Processes: An Evaluation Of Repetitive Negative Thinking And Hair Cortisol Concentration, Harrison Dickens

Graduate Theses and Dissertations

Habitual stress processes are thought to influence acute stress reactivity and stress-related disease. Habitual stress processes have been shown to adapt in response to stressor exposure experienced across the lifespan. The current study seeks to explore whether two habitual stress processes, hypothalamic-pituitary-adrenal (HPA) axis activity and repetitive negative thinking (RNT), were associated with lifetime stressor exposure (self-reported and objective neighborhood-level stressors). Additionally, the current study attempts to quantify how this process may be moderated by race and ethnicity. Participants (N = 134, Mage = 18 [SDage= 1.69], 56.7% female) consisting of White (N = 52), Black (N = 37 ), …

Improved Autofluorescence Lifetime Decay Analysis For Identification Of Cellular Autofluorescence Biomarkers For Label-Free, Single-Cell Assessment Of Mitochondrial Membrane Potential, Olivia Isabel Kolenc

Graduate Theses and Dissertations

Optical imaging of metabolism using the autofluorescence of the metabolic cofactors NADH, NADPH, and FAD has a wide range of sensitivities and the capability to collect a great deal of metabolic information. Multiphoton microscopy (MPM) is a non-invasive, label-free optical imaging technique capable of performing fluorescence lifetime imaging (FLIM) using time correlated single photon counting (TCSPC). However, autofluorescence FLIM presents a challenge in collecting sufficient photons to provide adequate signal to noise ratios (SNR) for analysis. Additionally, the specificity of autofluorescence biomarkers of metabolism has not been well-defined. Contextualizing these autofluorescence measures using established functional biomarkers can improve interpretability of …

Restoring A Longleaf Pine Savanna In The Wiregrass Gap: Community Responses To Post-Harvest Slash Manipulation, Eva Purcell

All Theses

Now reduced to just a fraction of their historical range, Longleaf pine savannas in the southeastern United States are home to a wide array of species, some of which are threatened or endemic. These characteristics have inspired widespread restoration initiatives in recent decades, accompanied by an expanding literature on how management techniques affect restoration outcomes. However, research is lacking in a unique region of longleaf pine’s range, where the dominant fire-carrying grasses are different: the Wiregrass Gap. This study investigated the effects of three different slash manipulation treatments on restoration outcomes of a Wiregrass Gap longleaf pine savanna. Specifically, we …

An Exploration Of Elderly Representation & Stereotypes In Animation, Tyler Kidd

All Theses

In the world of animated features, supporting cast members are often reduced down to simple character traits in order to give the audience a simple idea of their personality or role in the story, or to support the creation of a work that is easier to understand for people of all ages. However, this can often result in the application of negative tropes that do not reflect kindly on those being depicted. The elderly are a group that often get caught in these stereotypes, being depicted as wrinkly, clumsy, overweight villains. While you can still find many positive portrayals in …