Digital Commons Network^™

Egg White Hydrolysate In Hypertension: A Mechanistic Investigation Of Gut Microbiota Dependent And Independent Pathways, Emerson David Nolasco

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Cardiovascular diseases are one of the leading causes of mortality worldwide in which hypertension is a risk factor. Present pharmacological interventions require long-term adherence to the therapy and are often associated with undesirable side effects. Moreover, treatments have overlooked the role of the microbiome in the development of hypertension. Egg white hydrolysate (EWH) has exhibited antihypertensive properties with potential to be used as a natural alternative for the prevention and management of hypertension. However, EWH peptide’s effect on the gut microbiome remains yet to be elucidated. Studies have described that a portion of the ingested protein can remain undigested as …

Functional Genomics Of Phosphatidylcholine Biosynthesis In Saccharomyces Cerevisiae, Amanda Marie Maliva

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Phosphatidylcholine (PtdCho) is a prominent structural building block of cell and organelle membranes in animal, plant, and fungal cells and some bacteria. Due to its abundant nature in the plasma membrane and intracellular organelle membranes in eukaryotes, the biosynthesis of PtdCho must remain regulated to preserve the correct membrane lipid composition. Dysregulations in PtdCho metabolism can lead to mitochondrial dysfunction, cell death, and the development of diseases including cancer, atherosclerosis, and hepatic diseases. Knowledge of PtdCho has remained limited to the genes involved in its three biosynthetic pathways (Kennedy (K), methylation (M), and acyltransferase (A)), despite the growing realization of …

Characterization Of Undergraduate Biology Instructors’ Goals And Strategies For Implementing Quantitative Reasoning, Anum Khushal

School of Natural Resources: Dissertations, Theses, and Student Research

Quantitative reasoning (QR) is the application of mathematics and statistics to real-life and scientific contexts. It is essential for understanding complex biological phenomena and analyzing large datasets in both academic and professional settings. In response to the growing demand for QR skills, biology educators and researchers have engaged in curricular reforms and education research. This qualitative study explores how undergraduate biology instructors integrate QR into their teaching using a framework informed by pedagogical content knowledge (PCK) and QR. The study involved 21 biology faculty from various U.S. institutions who intentionally incorporated QR in their instruction. Data was collected through semi-structured …

Multiscale Drivers Of Water Level Fluctuations And Nutrient Availability In Small Intermittent Reservoirs In The Us Midwest, Uchechukwu Vitus Chinatu Ogbenna

School of Natural Resources: Dissertations, Theses, and Student Research

Reservoir dynamics are often captured by fluctuations in water surface elevation, which reflect a complex interplay of hydrologic inputs, climatic conditions, land use influences, and management operations. While water level fluctuations (WLFs) in large regulated systems have been widely studied, the drivers and implications of WLFs in small reservoirs, particularly those fed by intermittent streams, remain poorly understood. Here, we examined the temporal dynamics of WLFs and their relationship with nutrient availability in nine small reservoirs in the U.S. Midwest using daily records from 1985 to 2022. We used water level classification from modified Zscore and transition matrices to assess …

Exploring Racial Identity Among East And Southeast Asian Transracial Adoptees In The United States Through Communication Theory Of Identity, Brooke Marie Hoá Hornberger

Department of Communication Studies: Dissertations, Theses, and Student Research

The purpose of this study was to understand how East and Southeast Asian transracial adoptees (ESATA) in White adoptive families conceptualize and communicate their racial identity. ESATAs endure various challenges surrounding race that makes it important to understand how ESATAs understand their racial identity, the tensions they might experience, and how they management the tensions surrounding racial identity. Using the theoretical framework Communication Theory of Identity (Hecht, 1993), 25 ESATAs were interviewed about different frames of identity and potential identity gaps they experience surrounding racial identity. In addition, ESATAs were asked about how they communicatively manage identity gap tensions mapping …

Aptamer Selection And Development Of Electrochemical Aptamer-Based Sensors For Neuropeptide Y, Evelyn Adela Carreto Guevara

Department of Chemistry: Dissertations, Theses, and Student Research

In this thesis, we report the results of four aptamers obtained from an aptamer selection process called the systematic evolution of ligands by exponential enrichment (SELEX) with affinity to Neuropeptide Y (NPY). NPY is a highly abundant peptide transmitter with widespread distribution throughout the body which affects various physiological functions. These functions include energy homeostasis, appetite regulation, stress response, and anxiety regulation. Dynamic monitoring of NPY levels is crucial for better understanding behaviors in individuals with neurological disorders associated with NPY. Aptamers are synthetic oligonucleotides obtained through SELEX which have a binding affinity to a target of interest when in …

Artificial Intelligence On Campus: Revisiting Understanding As An Aim Of Higher Education, Jamie Herman, Henry Lara‐Steidel

Prindle Faculty and Staff Publications

AbstractThe launch of the powerful generative AI tool ChatGPT in November 2022 sparked a wave of fear across higher education. The tool could seemingly be used to write essays and do other work without students putting in the effort expected of them. In this paper, Jamie Herman and Henry Lara‐Steidel posit a way of addressing the concerns over ChatGPT and increasingly powerful generative AI tools in the classroom by first examining what exactly, if anything, widespread AI use undermines in education. That question, they argue, is logically prior to the question of what to do or how best to embrace …

Polymer-Enhanced Nanopore Sensing, Eugene Gyasi Agyemang

Graduate Theses and Dissertations

The resistive pulse technique characterizes single analytes by detecting fluctuations in the ion current as they pass through a narrow orifice connecting two electrolyte-filled chambers, each containing a single electrode. The amplitude, shape, frequency, and duration of these fluctuations primarily provide information about the analyte’s size, shape, concentration, and net surface charge, respectively. One configuration for resistive pulse measurements is of an electrolyte-filled nanopipette immersed in a bath, where the nanopipette tip acts as the nanoscale orifice. It was previously shown that incorporating the large quantities polymer polyethylene glycol (PEG 8K, 50% w/v) into the external electrolyte bath dramatically enhanced …

Data-Driven Koopman Theory For Transient Stability And Safety Analysis Of Power Systems With Renewable Penetration, Bhagyashree Umathe

All Dissertations

This dissertation presents a novel approach to analyzing and controlling nonlinear systems using the Koopman operator framework and data-driven methods. Nonlinear power systems, characterized by complex behaviors and sensitivity to initial conditions, pose significant challenges for stability and safety assessment, especially during transient events.

The first part of this work focuses on reachability analysis using the spectral properties of the Koopman operator. By leveraging eigenfunctions extracted from sampled trajectory data, the approach computes forward and backward reachable sets efficiently, even in high-dimensional nonlinear systems, without requiring dense state-space sampling. This method is validated through numerical examples, demonstrating its ability to …

Thermal Inkjet Bioprinting Of Human Fibroblasts Into Stem Cell Environment Leads To Stem-Like Gene And Protein Expression And Changes In Hippo Pathway Effectors Yap/Taz, Patricia Ablanedo Morales

Open Access Theses & Dissertations

Thermal inkjet bioprinting (TIB) has emerged as a powerful tool with many potential applications, such as organ regeneration, drug testing, and cell differentiation, among others. Despite the forces and stress that cells are subjected to during the printing process, there is little research that investigates in detail the combined effects of the TIB process and the surrounding environment on cells. Furthermore, a cell's biological environment greatly influences its behavior. Therefore, understanding the effects of bioprinting on cells in a particular environment at a genetic level can provide clues regarding changes in cell characteristics. Bioprinting could potentially be used as a …

Stress-Survival Pathway Genes In Colorectal Cancer: Crc Progression And Hispanic Disparities, Highlighting Mcm10 As A Potential Player, Urbashi Basnet

Open Access Theses & Dissertations

Despite advances in screening and treatment, colorectal cancer (CRC) remains a leading cause of cancer-related deaths in the U.S., with notable disparities across racial and ethnic groups. Hispanic populations face a disproportionate burden, underscoring significant differences in incidence and outcomes. This study examines the role of the Stress-Survival Pathway (SSP) in colorectal cancer (CRC), with a focus on addressing health disparities among Hispanic populations. Twenty-eight differentially expressed SSP genes identified from bioinformatic analysis were validated in CRC cell lines and cDNA arrays using qRT-PCR and tissue microarrays by immunohistochemistry. SSP genes were further analyzed in CRC tissues from Hispanic and …

Decoding Anisotropic Porous Medium: A Synergy Of Lattice Boltzmann Modelling And Operator Learning To Predict Permeability As A Function Of Orientation, Soumya Shouvik Bhattacharjee

Open Access Theses & Dissertations

Understanding the directional properties of porous media is essential for accurately predicting flow behavior, reactive transport, and fluid-solid interactions in systems ranging from geothermal reservoirs to energy storage devices and biological tissues. Directional variations in permeability - reflecting a medium's response to flow at different angular orientations - are particularly important for complex, inherently anisotropic geometries. In this study, we employ a Lattice Boltzmann (LBM) model to calculate directional permeabilities from porous media images subjected to varying flow inlet angles. Three classes of porous media were investigated: (1) synthetic media with circular grains, serving as isotropic baselines; (2) synthetic media …

Investigating The E74-Mediated Regulation Of A Gata Factor In Aedes Aegypti Mosquitoes, Jacklyn Marie Estrada

Open Access Theses & Dissertations

The mosquito species Aedes aegypti has a significant impact on public health as a vector for multiple pathogens. Its ability to transmit deadly diseases, including Yellow Fever, Dengue Fever, and Zika, emphasizes the urgency of understanding its reproductive biology. In female mosquitoes, reproductive success hinges on obtaining a vertebrate blood meal, which triggers vitellogenesis - the synthesis of yolk protein precursors (YPPs) necessary for egg development. Without a blood meal, female Ae. aegypti remain in a state of previtellogenic arrest, where the YPP gene vitellogenin (Vg) is repressed by a type of transcriptional factor, GATAr (AaGATAr) (Attardo et al., 2003). …

Environmental Drivers Of Vegetation Dynamics In Constructed And Natural Wetlands Of The Chihuahuan Desert, Enrique David Garcia

Open Access Theses & Dissertations

Desert wetlands are critical ecosystems that provide water, food, and habitat for many different species at different trophic levels. Additionally, they have the capacity to recharge aquifers, control floods, and purify water, among others. Unfortunately, they have been facing multiple environmental challenges due to different human activities, sometimes with disastrous consequences. There is an urgent need to better understand the structure and function of wetlands of different types and in different biomes in order to conserve, restore, and even create them so we can continue to obtain all the ecological services they provide. In this dissertation, I investigated how environmental …

Cellular And Noncellular Influences On Lipid Nanoparticle Tropism In The Liver, Mario Yomir Mata Corral

Open Access Theses & Dissertations

Hepatocellular carcinoma (HCC) is currently ranked as the second leading cause of cancer-related mortality worldwide, causing more than 700,000 deaths a year. The liver is important in blood filtration and metabolism, and for this reason is a prime target for drug delivery, with lipid nanoparticles (LNPs) showing promise as carriers due to their natural hepatic tropism. However, LNP efficacy is decreased in HCC due to the limited understanding of liver physiology in cancerous environments. This project proposes to address this gap by using a tissue-clearing technique known as CLARITY along with a chemically tagged LNP for comprehensive visualization of LNPs …

Spatial And Thermal Aspects Of The Ecology Of Three Sympatric Rattlesnakes In The Montane Northern Chihuahuan Desert, Joshua James Mead

Open Access Theses & Dissertations

To understand how animals coexist on the landscape, the individual aspects of a species life history must first be understood. Animals need to find locations that provide the resources they need to survive. Movement, microhabitats, and temperature can alter such areas where animals occur. Understanding animal movements provide important information on space use and microhabitats on the landscape. Ectothermic animals must acquiesce to ambient temperatures or use behavioral modifications to alter their body temperature. The ability to maintain suitable body temperature is essential for physiological function, reproduction, and ability to avoid predation. Research on the variation in a species ecology …

Effects Of Environmental Stressors On Human Tissue-On-A-Chip Platforms, Andie Padilla

Open Access Theses & Dissertations

As space exploration begins to extend beyond low earth orbit, it has become increasingly critical to understand the interaction of the extreme environment of space flight with human systems. While it is known that space-travel induces a vast array of complications to cardiac, neural, musculoskeletal, and immune systems, the mechanisms by which these complications occur are poorly understood. Current research to study the effects of microgravity and radiation are limited to ground simulations, which rarely account for the multifactorial stressors experienced during spaceflight, or long duration studies aboard the International Space Station. Similarly, traditional two-dimensional (2D) models lack the ability …

Expression Of Glycine Transporter 1 In The Rodent Basal Ganglia: Glial Vs. Neuronal, Rosa Atziri Perez

Open Access Theses & Dissertations

Glycine functions as an inhibitory neurotransmitter in the central nervous system (CNS),and it is mainly involved in autonomic functions. The levels of extracellular glycine are regulated by two membrane transporters, glycine transporter 1 and glycine transporter 2 (GlyT1 and GlyT2, respectively). These transporters differ in function and localization within the CNS. For many years, studies suggested that only GlyT2 served as a neuronal marker; however, GlyT1 has been localized in neurons in recent studies. The expression of GlyT1 is widespread compared to GlyT2; hence, the main objective is to determine the nature of the expression of GlyT1 in areas devoid …

Temporal Partitioning And Coexistence Among Four Sympatric Felid Species In The Northern Jaguar Reserve, Kylie Marie Rezendes

Open Access Theses & Dissertations

Coexistence among sympatric carnivores is shaped by behavioral strategies that mediate competition and mortality risk in heterogeneous landscapes. Understanding how sympatric carnivores, such as felids, use space and time in relation to one another requires models that account for both spatial and temporal overlap across landscapes. Models incorporating time-to-event probabilities provide a powerful framework for detecting behavioral dynamics across shared landscapes. This study evaluated the spatiotemporal responses of two apex predators â?? the jaguar (Panthera onca) and mountain lion (Puma concolor) - and two mesopredators - bobcat (Lynx rufus) and ocelot (Leopardus pardalis) - across five years of camera trap …

Experimental Investigation Of Photovoltaic Soiling Losses And Aerosol Absorption And Scattering Related To Dust In The Chihuahuan Desert Region, German Rodriguez Ortiz

Open Access Theses & Dissertations

This dissertation explores the interaction between mineral dust and solar radiation in the Chihuahuan Desert, one of the dustiest areas in North America. I focused on two main aspects: (1) the characterization of atmospheric aerosol optical properties during dust events, and (2) the effects of dust deposition (soiling) on the performance of photovoltaic (PV) modules. I characterized the intrinsic optical properties of aerosols in El Paso, Texas during the peak of the 2021 dust season. Ground-level, high-resolution optical and meteorological data were collected at the Black and Brown Carbon (BC^2) study site, whereas particulate matter and additional meteorological parameters were …

Humboldt County, Dennis Robert Sanchez

Open Access Theses & Dissertations

Danny Marquez, a police sergeant for the Los Angeles Police Department, takes his family on a vacation to Seattle, Washington. On the way, they stop in Humboldt County, and Danny is captivated by the beauty and serenity of this county. He imagines moving his family to Humboldt County and leaving the troubles of big city life behind. He applied for the position of Chief Inspector for the Humboldt Police. By the time he is hired, he loses both his son to Fentanyl and his wife to suicide. He is hired for the position but finds himself alone. He considers suicide. …

Phylogenetics And Integrative Taxonomy Of The Lang's Reed Frog Species Complex (Anura: Hyperoliidae: Hyperolius Langi), Dominic Troiani

Open Access Theses & Dissertations

The genus Hyperolius (Rapp, 1842) is the most speciose amphibian genus in sub-Saharan Africa. The genus has a complex taxonomic history because of its extensive intraspecific and interspecific morphological variation, large geographic distribution of many taxa, gaps in sampling, and the discovery of significant cryptic diversity within many species. The goal of this study is to understand the relationships and taxonomy of Hyperolius langi via an integrative taxonomic approach that combines molecular, morphological, and bioacoustics data to clarify the systematics of the geographically widespread species Hyperolius langi. Two mitochondrial (16S and cyt b) and one nuclear (RAG1) gene were used …

Laser Scan Path Design For Controlled Microstructure In Additive Manufacturing With Integrated Reduced-Order Phase-Field Modeling And Deep Reinforcement Learning, Augustine Twumasi

Open Access Theses & Dissertations

Laser Powder Bed Fusion (L-PBF) is a well-established additive manufacturing technique for fabricating intricate metal components with exceptional precision. A significant challenge in L-PBF is the formation of complex microstructures that influence final material properties. We propose a physics-guided, machine learning-aided approach to optimize scan paths for desired microstructure outcomes, such as equiaxed grains. We employed a phase-field method (PFM) to model the evolution of the crystalline grain structure. To reduce computational costs, we trained a surrogate machine learning model, a 3D U-Net convolutional neural network, using single-track phase-field simulations with varying laser powers to predict crystalline grain orientations based …

August 2025 Library News, University Libraries

Library News

No abstract provided.

Considering The Concept Of Law In The Legal Singularity, Matthew Tse

Canadian Journal of Law and Technology

This review article engages with the idea of a ‘‘legal singularity”, which has been developed by Abdi Aidid and Benjamin Alarie in their book, The Legal Singularity: How Artificial Intelligence Can Make Law Radically Better.

The legal singularity describes a legal system in the future that harnesses algorithmic computation to facilitate a ‘‘stable and complete legal order, capable of addressing and resolving practically all types of legal uncertainty in real time and on demand”. Aidid and Alarie believe algorithmic computation will achieve this promise in an interdependent dual process. On one hand, algorithms will structure the law with conceptual standardization. …

Pre-Service Teacher Perceptions Of An Outdoor Learning Experience Within A Science Methods Course, Steph Dean, Summer Landreth

Publications

Given the challenges currently facing early childhood science education, this exploratory study investigates the advantages of outdoor learning as part of a teacher preparation program. A group of 49 pre-service early childhood education teachers participated in a day-long outdoor learning experience embedded within their science methods course. Guided by the theoretical lens of embodied cognition, we employed a case study approach to collect and analyze qualitative survey data, using both categorizing and connecting strategies to explore participants’ experiences. The findings reveal four key themes related to pre-service teacher perceptions, in that outdoor learning: (1) represented an engaging experience, (2) contributed …

Making Sense Of Myc In Skeletal Muscle, Ronald G. Jones, Sebastian Edman, Nathan Serrano, Yuan Wen, John J. Mccarthy, Christopher S. Fry, Ferdinand Von Walden, Kevin A. Murach

Physiology Faculty Publications

No abstract provided.

Transferrin Receptor 1 Deficiency In Smooth Muscle Cells Attenuates Vascular Remodeling In Hypertensive Mice, Yoshiro Naito, Hisashi Sawada, Tetsuo Horimatsu, Masaki Ohmuraya, Masanori Asakura, Masaharu Ishihara

Physiology Faculty Publications

Transferrin receptor 1 (TfR1), a crucial cellular iron receptor, has a variety of biological functions. We have previously reported that TfR1 abundance increases in the aortic media of hypertensive rat models. However, its role in vascular diseases remains unknown. In the present study, we generated smooth muscle cell (SMC)-specific TfR1 deficient mice and examined the impact of its deletion in mouse models of hypertension induced by angiotensin II or deoxycorticosterone acetate/salt administration. SMC-specific TfR1 deletion attenuated medial thickening and elastin fragmentation in both mouse models of hypertension. These results indicate that TfR1 in SMCs exerts a role in vascular remodeling …

Advancing The Utility Of Unmanned Aerial Systems (Uas)-Based Imaging Techniques In Broadacre Agriculture: A Multimodal Case Study On Table Beets, Mohammad Shahriar Saif

Theses

Efficient and sustainable food production and management are growing concerns in the context of an ever-increasing global population. It is in this context that the integration of remote sensing with advanced imaging technologies presents transformative opportunities for data-driven decision-making in agriculture. This study therefore explores the use of unmanned aerial systems (UAS) equipped with multispectral, hyperspectral, and LiDAR sensors for the non-destructive monitoring of table beet (Beta vulgaris) crop traits, with a specific focus on root yield estimation and foliar disease assessment. Table beet, a subterranean crop of increasing commercial and nutritional importance, poses unique challenges for above-canopy sensing due …

Engineering Tunable Collagen Fiber Alignment Gradients To Quantify Contact Guidance Effects On Cell Migration, Indranil Mandar Joshi

Theses

Directional migration of cells is one of the most important physiological processes, necessary for tissue and organ development. It also plays a crucial role in pathological conditions such as cancer metastasis. This directed migration is influenced by various factors present in the native environment, which guide cells to migrate steadily in a specific direction. Due to its importance, over the years, a lot of emphasis has been placed on understanding and exploring the biochemical and biophysical factors that direct cell migration. In addition to the commonly studied biochemical and biophysical cues, contact guidance from collagen fibers, in the native matrix, …