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Unpacking Gender Inequality For Inclusive Development: A Case Study Of Myanmar Female Migrant Workers In Samut Sakhon Province, Thailand, Hsan Thawdar Htun Jan 2022

Unpacking Gender Inequality For Inclusive Development: A Case Study Of Myanmar Female Migrant Workers In Samut Sakhon Province, Thailand, Hsan Thawdar Htun

Chulalongkorn University Theses and Dissertations (Chula ETD)

The study aims to address the issue of gender inequality in Thailand's seafood industry, with a specific focus on Myanmar female migrant workers in Mahachai (Samut Sakhon province). Although women migrant workers play a significant role in Thailand's economy, they have been facing various challenges in low-wage industries. Gender inequality remains a prominent concern in these low-skilled labor-intensive workplaces like seafood industry. While there have been studies on migration that include both men and women or focus solely on women, gender has not received sufficient emphasis in understanding the experiences of female migrant workers, particularly in the seafood industry. This …

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Political Astronomy: South Korea Space Development Under The International Political Dimension, Nutnarin Ueasukpakdee Jan 2022

Political Astronomy: South Korea Space Development Under The International Political Dimension, Nutnarin Ueasukpakdee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human Knows about space technology development since 1950s era during that time U.S. and Soviet Union were main actors in “Space race” war. Space technology development of U.S. and Soviet Union in proxy war era were emphasized to develop for competition of political ideology and power of military. At the present time the purpose of space technology development different from the past, many countries that try to develop space technology for economic benefit, technology benefit, communication benefit or hidden connotation others. South Korea was the one that have potential and interesting to develop space technology because of the conflict with …

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Exploring Roles Of Mir-885-5p In Hepatocellular Carcinoma, Archittapon Nokkeaw Jan 2022

Exploring Roles Of Mir-885-5p In Hepatocellular Carcinoma, Archittapon Nokkeaw

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatocellular carcinoma (HCC), the most prevalent form of liver cancer, exerts a significant burden on Southeast Asian countries and stands as the third leading cause of cancer-related mortality worldwide. Despite this alarming impact, effective treatments for HCC are lacking, resulting in low survival rates and high recurrence rates. Therefore, a comprehensive understanding of the disease's underlying mechanisms is crucial for the development of novel and potent therapies. Recently, it has been recognized that microRNAs (miRNAs) play a vital role in tumorigenesis, including HCC. Our bioinformatic analysis has highlighted hsa-miR-885-5p as a potential candidate miRNA due to its downregulation in HCC …

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Exploring Roles Of Mir-372-3p In Proliferation Of Hepatocellular Carcinoma Cells, Pannathon Thamjamrassri Jan 2022

Exploring Roles Of Mir-372-3p In Proliferation Of Hepatocellular Carcinoma Cells, Pannathon Thamjamrassri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. HCC has a replicative immortality and sustained proliferation rate. In addition, cell cycle-related protein regulating proliferation in cancer are often found dysregulated, allowing cancer cells to proceed their proliferation uncontrollably. Recently, a small non-coding RNA, microRNA (miRNA), was found to play an important role in numerous biological functions. Specific miRNA may ameliorate or promote cancer progression through different target mRNA. MiR-372-3p has been explored in various cancers such as colon cancer, colorectal cancer, and glioma. However, its functions have been rarely studied in HCC, especially in the aspect …

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Classification Of Bacteria And Fungi In Peritoneal Dialysis Fluids Of Patients With Chronic Kidney Disease Based On Metagenomic Analysis, Suthida Visedthorn Jan 2022

Classification Of Bacteria And Fungi In Peritoneal Dialysis Fluids Of Patients With Chronic Kidney Disease Based On Metagenomic Analysis, Suthida Visedthorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chronic kidney disease (CKD) is a long-term condition where sustained damage of the renal parenchyma leads to the chronic deterioration of renal function that may gradually progress to end-stage kidney disease (ESKD). Peritoneal dialysis (PD) is a type of ESKD treatment that is beneficial to improve a patient's quality of life. However, PD-associated peritonitis is a major complication that contributes cause of death, and the detection of the pathogen provided a high culture-negative rate. This study aims to apply metagenomic approaches for identifying the bacteria and fungi in peritoneal dialysis effluent (PDE) of CKD patients based on the full-length 16S …

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Effects Of Chitosan Oligosaccharide And Probiotics On Chronic Kidney Disease Rats, Weerapat Anegkamol Jan 2022

Effects Of Chitosan Oligosaccharide And Probiotics On Chronic Kidney Disease Rats, Weerapat Anegkamol

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chronic kidney disease (CKD) patients suffer from the accumulation of toxic substances in their blood due to the loss of kidney function, which results in hyperphosphatemia. This condition contributes to hyperparathyroidism, leading to the development of chronic kidney disease-related mineral bone disorder (CKD-MBD). Additionally, CKD patients experience changes in their gut microbiota, disrupting epithelial tight junctions and allowing excessive absorption of dietary phosphate. In this study, we aimed to investigate the effects of various oligosaccharides and probiotics on the gut microbiota, intestinal barrier, hyperphosphatemia, and hyperparathyroidism in CKD rats. We isolated Lactobacillus and Bifidobacterium strains from healthy participants and tested …

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Study Of Thermodynamic Binding Between Ader And Ades In Two-Component Antibiotic Sensor Of Antibiotic Resistant Strains Of Acinetobacter Baumannii, Konrawee Thananon Jan 2022

Study Of Thermodynamic Binding Between Ader And Ades In Two-Component Antibiotic Sensor Of Antibiotic Resistant Strains Of Acinetobacter Baumannii, Konrawee Thananon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The proteins AdeR (response regulator) and AdeS (histidine kinase sensor) play a crucial role in regulating the efflux pump, which is responsible for removing antibiotics from bacterial cells. This regulation occurs through a two-component regulatory system (TCS) involving autophosphorylation. Upon receiving external signals, AdeS undergoes autophosphorylation, leading to the activation of AdeR, which in turn initiates the expression of target genes related to the efflux pump. In this study, both enzymes from the reference strain of Acinetobacter baumannii (ATCC19606) and antibiotic resistant strains (H1074 and G560) were cloned and expressed in E. coli. The enzymes were purified using an immobilized …

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Epigenetic Regulation In Trained And Tolerized Macrophages, Salisa Benjaskulluecha Jan 2022

Epigenetic Regulation In Trained And Tolerized Macrophages, Salisa Benjaskulluecha

Chulalongkorn University Theses and Dissertations (Chula ETD)

Innate immune memory is the phenomenon that can either enhance (trained) or suppress (tolerized) immune response by innate immune cells during the second encounter of the stimuli such as pathogens. Previous studies reported that epigenetic regulations critically regulate both types of innate immune memory. However, a complete understanding of the underlying mechanisms is still lacking. In this study, we performed a screening of an epigenetics compound library to identify inhibitors that affect ß-glucan (BG)-trained or LPS-tolerized macrophages. Among 181 compounds tested, various inhibitors targeting Aurora kinase, histone methyltransferase PRMT and EZH2, histone demethylase LSD1 and JMJD2, histone deacetylase 6 and …

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In Vivo Dosimetry Of 3d Gynecological Brachytherapy Using The Glass Dosimeter: A Phantom Study, Itsaraporn Konlak Jan 2022

In Vivo Dosimetry Of 3d Gynecological Brachytherapy Using The Glass Dosimeter: A Phantom Study, Itsaraporn Konlak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brachytherapy can deliver high doses to the target while sparing healthy tissues due to its steep dose gradient property. However, treatment was only performed according to the dose calculated by a treatment planning system without verification of the dose due to the characteristics. Therefore, the aim of this study was to design the in-house phantom to evaluate the dosimetric differences of gynecological brachytherapy under clinical conditions between calculation by the treatment planning system and measurement by the RPLGDs. An in-house phantom consisting of the glass dosimeter holder and the holder of the applicator was created. This holder was designed to …

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Accuracy In Classification Of Liver Lesion Ultrasound Using Artificial Intelligence, Sutthirak Tangruangkiat Jan 2022

Accuracy In Classification Of Liver Lesion Ultrasound Using Artificial Intelligence, Sutthirak Tangruangkiat

Chulalongkorn University Theses and Dissertations (Chula ETD)

B-mode ultrasound imaging is the standard method for hepatocellular carcinoma (HCC) screening. If a lesion has a unique appearance, a conventional detector can be applied. However, if the lesion shares its appearance with other lesion, a large training dataset is required, which may be unavailable. Therefore, a two-stage method is proposed. In the first stage, lesions are detected but not differentiated into any particular class. The detected lesions are then classified in the second stage using a conventional convolutional neural network (CNN). The aims of the dissertation are to design an artificial intelligent system model and to investigate the most …

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Accuracy Of Small-Field High Energy Photon Using Plastic Scintillation Detector In Heterogenous Media Compared With Model-Based Algorithm Calculation, Thanat Kaewsuksri Jan 2022

Accuracy Of Small-Field High Energy Photon Using Plastic Scintillation Detector In Heterogenous Media Compared With Model-Based Algorithm Calculation, Thanat Kaewsuksri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Some radiotherapy techniques such as stereotactic body radiotherapy (SBRT) has the challenges in small field dosimetry in heterogenous media. This study aims at evaluating the accuracy of W2 plastic scintillation detector (PSD) compared with the calculation from algorithms for small-field high energy photon in heterogenous media. The characteristics of W2-PSD were studied. The percentage depth dose (PDD) of 6 FFF and 10 FFF with various field sizes ranging from 1x1 to 4x4 cm2 were measured in solid water phantom with low-density inhomogeneities slabs of air (Styrofoam) and lung (cork) and high-density inhomogeneity slab of aluminum. The characteristics of W2 plastic …

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Regulatory T Cell In Drug-Induced Severe Cutaneous Adverse Reactions (Scars) During Acute And Recovery Phase, Suparada Khanaruksombat Jan 2022

Regulatory T Cell In Drug-Induced Severe Cutaneous Adverse Reactions (Scars) During Acute And Recovery Phase, Suparada Khanaruksombat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Drug reaction with eosinophilia and systemic symptoms (DRESS) is a type of SCARs induced by various drugs. The autoimmune consequences were observed throughout the recovery phase ranging between 10 – 20 % of DRESS patients. The dynamics of regulatory T cells (Tregs) are thought to be responsible for the many symptoms of DRESS. They may be a key player in developing autoimmune sequelae in this syndrome. This study concentrated on the number and function of CD4+CD25+CD127-FoxP3+ Tregs in autoimmune development in DRESS patients. CD4+CD25+CD127-FoxP3+ Tregs and their immunophenotyping were characterized using peripheral blood mononuclear cells (PBMCs) from patients by Flow …

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D-Lactic Acid Fermentation Platform From Oil Palm Trunk Sap By Sporolactobacillus Terrae Sbt-1, Naphatsawan Sukaiem Jan 2022

D-Lactic Acid Fermentation Platform From Oil Palm Trunk Sap By Sporolactobacillus Terrae Sbt-1, Naphatsawan Sukaiem

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lactic acid is an organic acid that contains two enantiomers, L- and D-lactic acid, and is considered the most potential monomer for many industrial products. Lactic acid has versatile applications in the pharmaceutical, food, textile, chemical, and leather industries moreover in poly-lactic acid (PLA) manufacturing. While L-lactic acid is well known in many commercial products and studies, only a few studies on D-lactic acid were found. Lactic acid can be produced either by microbial fermentation or chemical synthesis. Approximately 90 percent of the total lactic acid produced worldwide is via microbial fermentation which provides an optically pure L- or D-lactic …

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Phthalate Ester Biodegradation In Agricultural Soil Using Exogenous Bacterial Consortium Under Fertilizer Addition, Theodora Mega Putri Jan 2022

Phthalate Ester Biodegradation In Agricultural Soil Using Exogenous Bacterial Consortium Under Fertilizer Addition, Theodora Mega Putri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Phthalate esters (PAEs) have been classified as a priority contaminant due to their endocrine-disrupting properties and abundance in the environment. Previously, several PAE-degrading consortia were enriched from landfill soil. This study aimed to initially select a consortium with efficient degradation activity, to characterize based on degradation kinetics and community dynamics, and to evaluate bioaugmentation feasibility while maintaining activity through continuous transfer. It was obtained based on enriched consortium selection that LF-NK-DEHP could simultaneously degrade 92.9% 100 mg l-1 DBP and 63.4% 100 mg l-1 DEHP within seven days. Kinetic degradation revealed DBP degradation by LF-NK-DEHP to fit the modified Gompertz …

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Effect Of Normal Saline In Different Concentration On Soft Tissue Healing Of Extraction Wound: A Clinical Study, Walailuk Kunthikarn Jan 2022

Effect Of Normal Saline In Different Concentration On Soft Tissue Healing Of Extraction Wound: A Clinical Study, Walailuk Kunthikarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sodium chloride solution (NaCl) is regarded as the most suitable and recommended medical irrigation, because of the nontoxic and isotonic properties. The previous in vitro study showed that NaCl solution induced migration and extracellular matrix excretion from human gingival fibroblast. However, there is no report concerning the effect of rinsing extraction wound with NaCl solution in clinical trial study. This research study is split mouth single-blind randomized controlled clinical trial to investigate if rinsing with 0.9% and 1.8% NaCl solution on extraction wound can promote soft tissue healing. The operation was performed in 60 socket sites. The patients, who had …

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The Effects Of Decellularized Extracellular Matrix Derived From Jagged1-Treated Human Dental Pulp Stem Cells On Biological Responses Of Stem Cells Isolated From Apical Papilla, Suphalak Phothichailert Jan 2022

The Effects Of Decellularized Extracellular Matrix Derived From Jagged1-Treated Human Dental Pulp Stem Cells On Biological Responses Of Stem Cells Isolated From Apical Papilla, Suphalak Phothichailert

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in hDPSCs. This study aimed to investigate the characteristic of the Jagged1-treated hDPSCs-derived dECM and its biological activity on odonto/osteogenic differentiation SCAPs. Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed by NetworkAnalyst. hDPSCs seeded on Jagged1 immobilized surface were maintained with N or OM followed by decellularization procedure, dECM-N or dECM-OM, respectively. SCAPs were reseeded on each dECM with either normal medium or OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining and alcian blue staining. A Multipotential differentiation assay. …

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Effects Of The Orthodontic Miniscrew Geometric Design On The Bone Microdamage And Primary Stability, Panida Methawit Jan 2022

Effects Of The Orthodontic Miniscrew Geometric Design On The Bone Microdamage And Primary Stability, Panida Methawit

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigated the effects of the pitch (P) and thread shape on orthodontic miniscrew cortical bone microdamage. The relationship between microdamage and primary stability was examined. Ti6Al4V orthodontic miniscrews had custom-made thread heights (H) and pitch sizes and were classified into the following groups: HCPC (control); HCPN (with a narrower pitch); and HTPC (with a taller thread height) (HC = 0.12 mm; HT = 0.36 mm; PC = 0.60 mm; PN = 0.30 mm). The orthodontic miniscrews were inserted in the pilot hole of prepared 1.0-mm-thick tibia cortical bone, and the maximum insertion torque (MIT) and Periotest value (PTV) …

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Prediction Of Future Caries In Toddlers Via Salivary Microbiome: A 1-Year Longitudinal Study, Ratcha Raksakmanut Jan 2022

Prediction Of Future Caries In Toddlers Via Salivary Microbiome: A 1-Year Longitudinal Study, Ratcha Raksakmanut

Chulalongkorn University Theses and Dissertations (Chula ETD)

Dental caries in children is the most common chronic oral disease that could disturb their quality of life including their development. Early prevention is a key approach to reducing the prevalence of early childhood caries. However, a reliable caries predictor, as an essential tool for targeted prevention that is important to this approach, is still lacking for infants before caries onset. Therefore, we aimed to develop the caries risk prediction model based on the salivary microbiome of caries-free 1-year-old children to predict caries onset at 1-year follow-up. Using a nested case-control design within a prospective cohort study, 30 saliva samples, …

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Expression Of Superoxide Dismutase 3 (Sod3) During Orthodontic Tooth Movement In Rats, Phanchanit Jindarojanakul Jan 2022

Expression Of Superoxide Dismutase 3 (Sod3) During Orthodontic Tooth Movement In Rats, Phanchanit Jindarojanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hypoxia induces reactive oxygen species (ROS) production in periodontal tissues. Superoxide dismutase 3 (SOD3) is an enzyme protecting cells from ROS. This study investigated SOD3 expression and function during rat orthodontic tooth movement (OTM) and in hypoxia-exposed rat PDL cells. OTM of right maxillary first molars were performed in Sprague-Dawley rats for 1 and 14 days (n = 6 per group). SOD3 and HIF-1α expression were evaluated by immunohistochemistry. SOD3 effects on cell viability and proliferation, ROS production, and mRNA expression of Hif-1α, Rankl, and Opg in PDL cells and osteoclast differentiation were investigated under normoxia and hypoxia. SOD3 expression …

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The Effect Of Nano-Silver Fluoride In Remineralization On Artificial Dentine Caries: An In Vitro Study, Peeraya Punpeng Jan 2022

The Effect Of Nano-Silver Fluoride In Remineralization On Artificial Dentine Caries: An In Vitro Study, Peeraya Punpeng

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this in vitro study was to compare the remineralization efficiency among Nano-silver fluoride 400 ppm (NSF400), 38% Silver diamine fluoride (38% SDF) and 5% Sodium fluoride (5% NaF) on artificial dentine caries in permanent molar or premolar teeth. The teeth were sectioned to obtain 120 specimens (3 x 3 x 2 mm3). After artificial caries were created, the lesion depth (LD) was measured using Micro-computed tomography (Micro-CT) (n=15 specimens/group), and the surface microhardness (SMH) was measured using a Knoop microhardness tester (n=15 specimens/group). Subsequently, all specimens were randomly assigned to four groups: Group 1) NSF400, Group 2) …

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Evaluation Of Different Scaffolds For Bone Regeneration In Rat Calvarial Bone Defects, Jeerawit Sirakittiworapong Jan 2022

Evaluation Of Different Scaffolds For Bone Regeneration In Rat Calvarial Bone Defects, Jeerawit Sirakittiworapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Scaffold plays a key role in the context of tissue engineering. It not only provides a structural support for cells, but also creates an appropriate milieu for recruited cells, enhancing the therapeutic effects of cell-based treatments, and enabling the controlled release of biological cues such as growth factors. Collagen is widely used scaffold material in tissue engineering, particularly in periodontal and bone regeneration. Other natural polymers, however, have been developed and can be useful. Therefore, in this study, we aim to investigate the ability of four different natural polymers including collagen, chitosan, silk fibroin, and silk fibroin/gelatin hydrogel in promoting …

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Protein Expression After Gingival Injection Of Messenger Rna Encoding Platelet-Derived Growth Factor-Bb In Ligature-Induced Rat Periodontitis, Pimphorn Meekhantong Jan 2022

Protein Expression After Gingival Injection Of Messenger Rna Encoding Platelet-Derived Growth Factor-Bb In Ligature-Induced Rat Periodontitis, Pimphorn Meekhantong

Chulalongkorn University Theses and Dissertations (Chula ETD)

As a new class of medicines, mRNA platform has proven to be safe and effective. The objective of this study was to investigate the effect of local inflammation on platelet derived growth factor-BB (PDGF-BB) expression after gingival injection of PDGF-BB mRNA in lipid nanoparticles (LNPs) in ligature-induced rat periodontitis and to examine the dose-dependent effect of gingival delivery of mRNA. 3-0 silk was placed around maxillary left second molar for two weeks and removed, while the corresponding molar on the right was non-ligated. Under stereomicroscope, a significant bone loss at ligature sites was observed as compared to the non-ligature sites, …

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Application Of Core Annular Flow For Oil Transportation In Different Pipe Configurations Using Computational Fluid Dynamics, Cindy Dianita Jan 2022

Application Of Core Annular Flow For Oil Transportation In Different Pipe Configurations Using Computational Fluid Dynamics, Cindy Dianita

Chulalongkorn University Theses and Dissertations (Chula ETD)

The application of Core Annular Flow (CAF) has become an interesting solution in transporting heavy oil through pipeline because of its energy reduction and cost efficiency. Current study conducted a 3D computational fluid dynamic (CFD) to simulate CAF of oil-water in horizontal T- and Y-pipe junctions with two types of oil characteristic i.e., oil as Newtonian fluid and oil as non-Newtonian Carreau Fluid. The 2k factorial statistical experimental design was applied to investigate the effect of geometry on the flow performance. Eight cases were run with different diameter combinations and junction angle. The most attractive design was measured by the …

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Preparation Of Cellulose Micro- And Nano Fibers From Bagasse Through Deep Eutectic Solvent System, Worapoj Phuckpetch Jan 2022

Preparation Of Cellulose Micro- And Nano Fibers From Bagasse Through Deep Eutectic Solvent System, Worapoj Phuckpetch

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to prepare the cellulose micro- and nanofibers (CMNFs) using green chemistry viewpoint. The CMNFs were fabricated from sugarcane bagasse (SCB) pulp through treatment with zinc chloride (ZnCl2) - lactic acid (LA) in deep eutectic solvent (ZnCl2-LA DES) followed by short time ultrasonication of the DES-treated fibers in water. Under the optimum condition, the SCB pulp at high solid loading of 6.25% was treated with ZnCl2-LA DES. Lactate functionalized CMNFs with 230-270 nm length and 15-16 nm width were resulted in more than 90% yield and up to 62.2% crystallinity. The results showed that ZnCl2-LA DES was a …

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Stimulation Of Immune Response In Mice Using The Plant-Produced Sars-Cov-2 S1 Subunit Protein Linked To The Fc Region, Chalisa Panapitakkul Jan 2022

Stimulation Of Immune Response In Mice Using The Plant-Produced Sars-Cov-2 S1 Subunit Protein Linked To The Fc Region, Chalisa Panapitakkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 made a devastating impact worldwide, and virus management is challenging owing to a lack of specific therapies or affordable vaccines, especially in low-and middle-income countries. Hence a low-cost and efficient vaccine is necessary. In the present study, the immunogenicity of the plant-produced S1 subunit protein of SARS-CoV-2 was investigated in order to utilize it as a subunit vaccine. The SARS-CoV-2 S1 subunit protein was fused with the Fc region of human immunoglobulin (IgG1) and expressed in tobacco plants in species of Nicotiana benthamiana plants by agroinfiltration. The …

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Identification Of Potential Drug Targets For Covid 19 Patients With Cytokine Storm, Bayu Cakra Buana Jan 2022

Identification Of Potential Drug Targets For Covid 19 Patients With Cytokine Storm, Bayu Cakra Buana

Chulalongkorn University Theses and Dissertations (Chula ETD)

The outcome of SARS-CoV-2 infection may vary depending on gender, lifestyle, and age. The elderly is more likely to have the severe condition than the younger population. However, several young people suffer from severe lung diseases caused by cytokine dysregulation, also known as a "cytokine storm." Cytokine storms are associated with Covid-19 severity and mortality. Identification of potential cytokine storm targets is critical for improving patient survival. Transcriptomic analysis of expression data from publicly available databases could be used to identify differentially expressed genes and pathways. The list of both results was used to narrow down the potential candidates in …

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Renieramycin T Derivative Dh_22 Induces Apoptosis In Lung Cancer Cells By Downregulation Of C-Myc Protein, Indiana Gita Anggraeni Jan 2022

Renieramycin T Derivative Dh_22 Induces Apoptosis In Lung Cancer Cells By Downregulation Of C-Myc Protein, Indiana Gita Anggraeni

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung cancer is a prevalent cause of death, indicated by an elevated c-Myc level. We synthesized a new derivative of renieramycin T (RT), named DH_22, and tested it for anti-cancer activities in human lung cancer cells. In the cell viability and nuclear staining tests, DH_22 was discovered to be cytotoxic, with an IC50 of around 13 μM; and found to mediate apoptosis in the lung cancer cells. Meanwhile, in a mechanistic sense, DH_22 contributed to the activation of p53-dependent apoptosis and decreased the cellular level of c-Myc. The p53-dependent mechanism was indicated by an increase of p53, an induction of …

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Development Of Surrogate Sars-Cov-2 Virus Neutralization Test Method Using Plant-Produced Recombinant Proteins, Perawat Jirarojwattana Jan 2022

Development Of Surrogate Sars-Cov-2 Virus Neutralization Test Method Using Plant-Produced Recombinant Proteins, Perawat Jirarojwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but a conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and a pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals in BSL2. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme (ACE2) produced from Nicotiana benthamiana for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2-His can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with …

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Development Of Plant-Produced Subunit Vaccines To Elicit The Immunogenicity Against Sars-Cov-2 Variant Viruses, Narach Khorattanakulchai Jan 2022

Development Of Plant-Produced Subunit Vaccines To Elicit The Immunogenicity Against Sars-Cov-2 Variant Viruses, Narach Khorattanakulchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Since the emergence of the coronavirus pandemic in 2019 (COVID-19), the development of effective vaccines to combat the infection has accelerated worldwide. While the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) with mutations in the receptor-binding domain (RBD) with high transmissibility, enhanced infectivity, and immune escape from vaccination is also predominantly emerging. Effective vaccines against variant of concern (VOC) and optimized booster vaccination strategies are thus highly required. Here, the gene encoding seven different RBD (ancestral (Wuhan), Alpha, Beta, Gamma, Kappa, Delta, and Epsilon variants) fused with the fragment crystallizable region (Fc) of human IgG1 (RBD-Fc) was constructed and cloned into …

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Predictors Of Fertility Quality Of Life In Infertile Patients Visiting Infertility Center In Kathmandu, Nepal : A Cross-Sectional Study., Shital Shakya Jan 2022

Predictors Of Fertility Quality Of Life In Infertile Patients Visiting Infertility Center In Kathmandu, Nepal : A Cross-Sectional Study., Shital Shakya

Chulalongkorn University Theses and Dissertations (Chula ETD)

In Nepal, infertility is a rising reproductive health issue with an estimated prevalence of 15%. There are few studies on quality of life in infertile women, but none on quality of life in infertile men and women although infertility is a shared condition and has effects on couples. The study objective is to describe the various predictors of infertile patients seeking infertility treatment and find association between these predictors and the quality of life. A cross-sectional study using a self-administered disease specific FertiQoL questionnaire was conducted among 409 infertile men and women seeking infertility treatment in an infertility center in …

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