Digital Commons Network^™

Selective Transfection Of A Transferrin Receptor-Expressing Cell Line With Dna-Lipid Nanoparticles And Synthesis Of Parasite-Derived Glycans As Biomarkers For Leishmaniasis, Irodiel Vinales Lozano

Open Access Theses & Dissertations

Despite notable progress in lipid nanoparticle (LNP)-mediated gene delivery, achieving selective transfection of specific cell types, such as cancer cells, remains a significant hurdle, hindering the advancement of innovative gene therapies. In this study, we engineered an LNP formulation encapsulating plasmid DNA (pDNA) encoding the monomeric Green Lantern (mGL) fluorescent reporter protein. The DT7 peptide ligand targeting human transferrin receptor 1 (hTfR1) was also conjugated to the LNP surface for targeted delivery to hTfR1-expressing cells. Optimization of LNP composition yielded favorable particle diameter, ζ-potential, yield, and pDNA encapsulation efficiency. Evaluation of transfection selectivity using a panel of two engineered cell …

Resource Scarcity Caused By Environmental Changes: Driving Factor In Terrorism Attacks In Afghanistan, Pakistan, Syria, Amaris Bustamante

Open Access Theses & Dissertations

Climate change, resource scarcity, and terrorist attacks are ever-growing crises that disproportionately impact different states. They are crises that can impact the stability and resilience of humanity in the following decades if they are not addressed and mitigated. This study addresses the impact of resource scarcity caused by climate change that can then serve as a driving force in terrorist attacks in climate-sensitive and conflict-prone states. The objective of this mixed-methods study is to identify the correlation between climate changes that lead to resource scarcity such as rainfall and surface temperatures with terrorist attacks when taking into consideration other demographic, …

In-Between Frame Generation For 2d Animation Using Generative Adversarial Networks, Francisco Arriaga Pazos

Open Access Theses & Dissertations

Traditional 2D animation remains a largely manual process where each frame in a video is hand-drawn, as no robust algorithmic solutions exist to assist in this process. This project introduces a system that generates intermediate frames in an uncolored 2D animated video sequence using Generative Adversarial Networks (GAN), a deep learning approach widely used for tasks within the creative realm. We treat the task as a frame interpolation problem, and show that adding a GAN dynamic to a system significantly improves the perceptual fidelity of the generated images, as measured by perceptual oriented metrics that aim to capture human judgment …

On Hannah Arendt's Study Of Constitutionalism In The Aftermath Of Totalitarianism: A Philosophical Search For The Principle To Secure The Foundations Of Modern Politics, Joseph De Leon Flores

Open Access Theses & Dissertations

Hannah Arendt faces the grotesque appearance of totalitarianism, and with bravery goes on the offensive, herself armed with contemporary philosophical tools of analysis, to do battle in the field of existential-phenomenology against this modern monster. Totalitarianism, birthed from the seeds of lawless action, claims to be the most lawful mode of human existence. The monstrous existence of totalitarianism demonstrates a crisis in the very foundations of modern manâ??s political mode of being. In order to find a solution to this modern political crisis Arendt closely studies the experience of constitution writing at the moment the men of action are about …

Iron Curtains: A Novel Of Germany, 1989, John G. Dodson

Open Access Theses & Dissertations

A work of fiction. A story of Love, Treachery, and Freedom on the East German border in 1989. Iron Curtains is a story of four individuals in East and West Germany brought together by an espionage investigation and the revolution in East Germany which led to the fall of the Berlin Wall and Iron Curtain on November 9, 1989. Their stories ultimately intersect as the year passes and culminate in an escape through a tunnel underneath the East German border. There are four main characters and several secondary characters who help drive the story forward by their interaction with the …

Assessing Urban Tree Coverage Along The U.S.-Mexico Border: A Gis Analysis Of Paso Del Norte, Melanie Escobar

Open Access Theses & Dissertations

In recent years, researchers have extensively studied the spatial distribution of social demographics and urban tree canopy (UTC) in urban cities, but very few, to this date, address U.S.-Mexico border cities. To date, there is no research that assesses the distribution of urban tree canopy (UTC) in the city of El Paso, Texas, and Ciudad Juarez, Chihuahua, along the U.S.- Mexico border. Leveraging advanced mapping techniques and GIS tools, the study performs comparisons between countries (Juárez vs. El Paso urbanized areas and intra-country (within each country). It compares land cover classifications, assesses variations in UTC distribution across census tracts and …

Relations Between Printability And Rheology In A Ceramic Paste Extrusion Process, Nicholas Durand

Open Access Theses & Dissertations

Ceramic additive manufacturing (AM) uses the same fundamentals as material extrusion (MEX) that utilizes polymers or resins for printing applications. Ceramic AM uses the same process of slicing an STL file to determine a toolpath for the printer that will facilitate material deposition to manufacture full parts; ceramic AM also provides a low-cost advantage when compared to processes that require high powered lasers for melting metal powders or heated nozzles used for dispensing thermoplastics. Despite having the similar design freedom and ability for rapid prototyping that other printing methods benefit from with the addition of low cost, ceramic AM has …

Analysis Of Additively Manufactured Inconel 718 Combustion Behavior In Promoted Oxygen Environments, Dominic George Dieguez

Open Access Theses & Dissertations

Promoted combustion testing is a vital tool for engineers to establish the combustion and flammability characteristics of materials (metallic or otherwise) in oxygen enriched environments. Historically, much of the established data for metallic promoted combustion has been with regards to materials in their cast and wrought forms. However, with the emergence of additive manufacturing as a preferred method of fabrication, the need exists to evaluate how metals in that form behave. Recent testing has demonstrated that even if a metal or alloy is nominally the same with regards to chemistry, flammability between samples in the wrought form can differ significantly …

Workplace Bullying In K-12 Education, Lorena Garcia

Open Access Theses & Dissertations

Workplace bullying within K-12 education settings has become an increasingly recognized concern, with potentially profound implications for educators' well-being. This quantitative research study aims to explore the phenomenon of workplace bullying among teachers and administrators in K-12 educational institutions. Findings from this study will contribute to the existing literature by shedding light on the prevalence and manifestations of workplace bullying in K-12 education, as well as its implications for individual well-being, organizational culture, and educational outcomes. The study aims to inform the development of targeted interventions and policies to prevent and address workplace bullying, thereby fostering healthier and more supportive …

Promoting Social Sustainability: Implementing A Dynamic Stem Driven Framework To Empower Underrepresented Communities In West Texas Schools, Raquel Haggerty

Open Access Theses & Dissertations

This dissertation explores how environmental engineering can utilize STEM education as a strategic tool, enhancing social sustainability and thereby empowering underserved Hispanic communities in West Texas. Responding to significant educational and resource disparities, this research introduces an innovative interdisciplinary framework that combines instruction-initiated resource activation (I2RA), culturally attuned parent engagement (CAPE), and community- centered STEM education (CCSE). This approach uniquely addresses the challenges faced by these communities, integrating environmental solutions with educational advancements to forge a path toward equitable STEM access and achievement.

Additionally, the "Elevate and Empower" case study exemplifies the practical application of this comprehensive framework, demonstrating its …

Manipulation Of The Magnetic Properties Of Van Der Waals Materials Through External Stimuli, Luis Martinez

Open Access Theses & Dissertations

A new revolutionary application dependent on the electron spin to carry information with greater efficiency in data storage, transfer, and processing, will rely heavily on 2D magnets and the ability to effectively control their electron spins and engineer their properties. Previously, magnetic thin films were heavily studied to achieve this goal, however, these materials came with pitfalls and lacked naturally occurring 2D magnetism. The recent discovery of intrinsic magnetism in few-layered van der Waals (vdW) magnets has inspired researchers to extensively study them because of the feasibility to exfoliate them down to a monolayer. Due to this dimensionality factor, vdW …

Computational Study Of Fluorescent Molecular Probes For The Early Detection Of Alzheimer's Disease, Gabriela Elizabeth Molina Aguirre

Open Access Theses & Dissertations

Alzheimer's disease is a debilitating brain disorder that affects memory, thinking, and behavior. It is the most prevalent form of dementia and the seventh leading cause of death in the United States. As, researchers have identified biomarkers that can indicate the presence of the disease many years before the first symptoms appear, so there is an opportunity for early detection and treatment follow-ups, which could significantly improve the quality of life for those affected by this disease.This dissertation investigates the relationship between molecular structure and optical properties of donor-bridge-acceptor (DBA) fluorescent molecular probes designed for detecting Amyloid-β and p-Tau protein …

Dynamic Storytelling Algorithms Using Contextual Aspects Of A Large Language Model, Alireza Pasha Nouri

Open Access Theses & Dissertations

Storytelling is a set of algorithms used to create narratives by connecting documents in a sequencethat accurately reflects the evolution of events and entities within a particular topic or theme. Early storytelling algorithms face challenges in encoding the progression and interconnections of information between consecutive texts, given that the conventional approaches rely primarily on connecting document pairs based on content overlap. They often neglect critical linguistic features, such as word contexts, semantics, the roles words play across different documents, and attention to the historical contexts of the underlying documents. Many existing storytelling models frequently produce story chains that, while connected …

Energy Optimization In Pneumatic Systems Using Ens-200 Energy Saving Trainer, Maha Guru Shankar Perumal

Open Access Theses & Dissertations

Various industries such as manufacturing and assembly, the automotive sector, printing and paper handling, packaging, aerospace, etc., widely use pneumatic systems for a variety of operations. Enterprises use pneumatic systems for cost-effectiveness, reliability, and simplicity. However, they also have certain inefficiencies, leading to high operating costs and reduced efficiency in various applications. The focus of this research is on energy optimization in pneumatic systems to improve efficiency in industrial applications, minimize energy waste, and reduce operational costs. The best practices for enhancing energy efficiency in pneumatic systems were proper system design, pressure regulation, leak detection and repair, energy-efficient components, and …

Investigating Changes In Surface Water Chemistry Across The Llano Uplift And The Balcones Fault Zone In Central Texas Using Strontium And Uranium Isotopes, Hao Tuan Pham

Open Access Theses & Dissertations

The landscape of central Texas is shaped by over one billion years of Earth's history. An array of lithology lies within central Texas along with two large geological structures. The Llano Uplift, characterized by Proterozoic metamorphic and igneous rocks dating back to 1.37 billion years ago, is encircled by Paleozoic and Mesozoic sedimentary rocks. To the southeast of the Llano Uplift lies the Balcones Fault Zone (BFZ), an extensional structural system of mostly normal faults that delineates the transition between Paleozoic-Mesozoic sedimentary rocks and Cenozoic sedimentary cover, which are influential to the regional hydrogeology. This study investigates the hydrogeological dynamics …

Mav Localization In Gps-Denied Environments And Synthetic Data Collection In Challenging Simulated Conditions, Julio A. Reyes Munoz

Open Access Theses & Dissertations

The development of unmanned aerial systems presents an opportunity for conducting industrial inspections in environments where traditional navigation systems, such as the Global Navigation Satellite System (GNSS), are compromised. This dissertation investigates the implementation of a micro aerial vehicle (MAV) capable of autonomous data acquisition in complex, GNSS-degraded industrial settings. The primary challenge addressed is the robust localization of MAVs, a critical aspect in ensuring reliable operation under varying and uncertain environmental conditions.

The work is divided into two main parts. The first part focuses on the design and integration of a MAV system specifically for power plant inspections in …

Synthesis Of Neoglycoproteins As Potential Biomarkers For Chagas Disease And Cutaneous Leishmaniasis Caused By Leishmania (Viannia) Braziliensis, Eileni Rodriguez Gil

Open Access Theses & Dissertations

Protozoan parasites are responsible for causing numerous diseases in humans and mammals worldwide. Among them are Chagas disease (CD), caused by the parasite Trypanosoma cruzi (T. cruzi), and tegumentary leishmaniasis (TL), caused by the parasite Leishmania (Viannia). Both diseases coexist in many regions of Latin America, and current hurdles to differentially diagnose them and determine chemotherapy success need to be overcome. CD can be diagnosed by traditional serological tests and polymerase chain reaction (PCR), but disadvantages of these methods are that they may produce false negatives because the antigens used in commercial CD kits are not conserved across all T.cruzi …

Produced Water: Characterization And Treatment, Ramon Antonio Sanchez Rosario

Open Access Theses & Dissertations

In recent years, environmental concerns have urged companies in the energy sector to modify their industrial activities to facilitate greater environmental stewardship. For example, the practice of unconventional oil and gas extraction has drawn the ire of regulators and various environmental groups due to its reliance on millions of barrels of fresh water for hydraulic fracturing well stimulation, which is generally withdrawn from natural sources and public water supplies. Additionally, this process generates two substantial waste streams, which are collectively characterized as flowback and produced water. Whereas flowback water is comprised of various chemical additives that are used during hydraulic …

Characterizing The Mentoring Landscape In An Online Biology Cure: An Examination Of Student And Graduate Teaching Assistant Perspectives And Outcomes, Karen Alejandra Santillan

Open Access Theses & Dissertations

Both undergraduate research experiences (UREs) and course-based undergraduate research experiences (CUREs) provide students with benefits and opportunities such as improving technical skills and making connections that provide resources. Where these two experiences differ is accessibility. Similar to their URE counterpart, CUREs entail mentorship on the part of the faculty/graduate teaching assistant (GTA) instructor (Dolan, 2016). There is general agreement that mentoring has many benefits for undergraduate STEM students, such as an improvement in retention rates, increased confidence and self-efficacy, and a more established sense of belonging and science identity (Dolan, 2016). CURE instructors often adopt the role of “mentor” in …

Physics-Guided Scan Paths Optimization For Controlled Microstructure In Laser Powder Bed Fusion, Augustine Twumasi

Open Access Theses & Dissertations

Laser Powder Bed Fusion (L-PBF) is a renowned additive manufacturing technique, celebrated for its capability to construct intricate metal components with remarkable precision. However, one of the main challenges with L-PBF is the formation of complex microstructures, which can significantly impact the final material properties. To address this issue, our study proposes a physics-guided and machine-learning-aided approach to optimize scan paths for achieving desired microstructure outcomes, such as the generation of equiaxed grains that enhance material properties. By using phase-field modeling, a physics-based computational method, we gain insights into microstructure evolution. To reduce computational costs, we train a surrogate machine-learning …

Automated Composition Of Multivariable Scientific Workflows Considering Scientific Assumptions, Raul Alejandro Vargas Acosta

Open Access Theses & Dissertations

Many ground-breaking scientific experiments require the execution of multiple complex scientific computations. Thus, scientific workflows (i.e., a sequence of scientific computations) have received significant attention, more specifically, the automated composition of scientific workflows. Scientific workflows that repurpose data may have unique scientific assumptions that need to be considered when composing a workflow. Workflow composition tools have enabled a wider range of stakeholders (e.g., policymakers, the general public, and researchers) to create and execute workflows; however, domain expertise is still required for these tasks. The overarching goal of this work is to further improve the automatic composition of scientific workflows by …

Race, Severe Mental Illness, And Crime: An Intersectional Look Into Stigma And Policy Implications, Elena Therese Vaudreuil

Open Access Theses & Dissertations

Criminal behavior has been a long-discussed topic in the United States and often is tied to characteristics such as race and mental illness. The presumed connection between criminal behavior and being a member of a racial minority group or having a mental illness have been researched for years, however few researchers have sought to take an intersectional approach to investigate the unique experiences of people belonging to both groups in the criminal legal system. Using the lenses of attribution and intersectionality theories, the proposed studies sought to understand the effect of race that influences policy support of justice-involved people with …

Analyzing The Impact Of Oil And Gas-Contaminated Fluids On Soil In Eddy And Lea Counties Using Sequential Extraction, Mary Adu-Gyamfi

Open Access Theses & Dissertations

The study investigates the impacts of oil and gas-contaminated fluids on soil within the context of Eddy and Lea Counties in New Mexico, the United States. The increase in oil and gas operations has led to enormous wastewater production that is handled, stored, and transported, leading to thousands of spills yearly. The continuous and accidental release of this wastewater poses several risks to the environment due to its high levels of hazardous pollutants. Several studies were conducted on how to reduce the contaminants; however, the impact caused on the environment and the fate of the spilled fluids is unclear due …

Nationwide Practices And Perspectives On Early Literacy Activities In Daycare, Analaura Amador

Open Access Theses & Dissertations

No abstract provided.

Dispersal And Population Structure Of Euchlanis Chihuahuaensis Via Anemochory In The Chihuahuan Desert, Tristan Chavez-Poeschel

Open Access Theses & Dissertations

Desert ecosystems present challenges for aquatic organisms as habitats are fragmented, both in space and time; however, diapausing stages of rotifers can travel hundreds of kilometers during wind events. I used the rotifer Euchlanis chihuahuaensis as a model species to investigate the influence of wind dispersal on gene flow and population genetics in Chihuahuan Desert populations. I hypothesized that anemochory facilitates gene flow from source populations in the Northern Chihuahuan Desert in Mexico and the western United States to habitats in the Trans-Pecos region via delineated wind corridors. To test this hypothesis, the genetic diversity of populations from both inside …

Water Sufficiency For Organismal Function In Dryland Critical Zones, Lindsey Dacey

Open Access Theses & Dissertations

Water availability is crucial for organismal survival and growth in dryland environments, affecting both ecological interactions and carbon dynamics. The goal of this thesis is to develop soil water release curves (SWRCs) that link soil water potentials (Ψ) to soil water content (θ). Using the SWRCs, temporal soil water sufficiency curves are developed, which quantify the amount of time that dryland critical zones have enough water to sustain the physiology of organisms. These curves allow for effectively indicating water availability across different species, coverage types, and soil conditions, enhancing our understanding of water dynamics in drylands and contributing important parameters …

Depositional Facies And Petrophysical Characterization Of The Leonardian Carbonate (Lower Clear Fork), Tex-Mex, S.E. Field, Central Basin Platform, West Texas, Seyram Nyamasekpor

Electronic Theses and Dissertations

The Lower Clear Fork from Tex-Mex, S.E. Field on the Central Basin Platform is a typical complex reservoir that displays high heterogeneity in lithological and petrophysical properties. The unit represents a producing reservoir succession of Leonardian platform carbonates deposited in shallow marine water during the Early Permian. The sediment of the Lower Clear Fork is composed of a mixed succession of dolomite interbedded with anhydrite, minor clay minerals, and siliciclastics. The high heterolithic nature of the reservoir makes efficient recovery of hydrocarbons difficult. This situation requires an understanding of the variability in depositional facies in terms of mineralogy, depositional textures …

Formalization Of A Security Framework Design For A Health Prescription Assistant In An Internet Of Things System, Thomas Rolando Mellema

Electronic Theses and Dissertations

Security system design flaws will create greater risks and repercussions as the systems being secured further integrate into our daily life. One such application example is incorporating the powerful potential of the concept of the Internet of Things (IoT) into software services engineered for improving the practices of monitoring and prescribing effective healthcare to patients. A study was performed in this application area in order to specify a security system design for a Health Prescription Assistant (HPA) that operated with medical IoT (mIoT) devices in a healthcare environment. Although the efficiency of this system was measured, little was presented to …

Compound Marine Cold Spells And Hypoxic Events In A Nearshore Upwelling System, Ryan K. Walter, Michael Dalsin, Piero L.F. Mazzini, Cassia Pianca

Physics

Prolonged periods of extreme warm and cold seawater temperature anomalies, known as marine heatwaves (MHWs) and marine cold spells (MCSs), respectively, can have significant impacts on coastal marine ecosystems. Prior research has examined the co-occurrence of multiple extremes [low dissolved oxygen (DO) and/or low pH] during MHWs, with the impact of these compound events potentially exceeding that of a single stressor due to synergistic effects. However, we are not aware of any studies that have examined compound extreme events during MCSs, despite the ecosystem implications. Along Eastern Boundary Current Upwelling Systems (EBUS), strong-wind driven upwelling has led to …

An Inconvenient Truth About ‘Bundling’ Commitment, Engagement, & Embeddedness: Unbundling To Extend Theory On Turnover Motivations And Beyond, Carl Maertz, Clark D. Johnson, Brittney C. Bauer

School of Business: Faculty Publications and Other Works

This paper critiques a research practice that we call “bundling,” which has produced highly popular constructs in the organizational behavior literature, including organizational commitment, employee engagement, and organizational embeddedness. We show how these bundled constructs, using broad labels from common parlance, have produced overlapping meanings, confounded theoretical mechanisms, and imposed limiting “ideal employee” conceptions in the literature, organizations, and ultimately, societal discourse about employees. We argue that “unbundling” these constructs can provide multiple benefits to theory, empirical inquiry, and practical assessment of complex employee motives. As a demonstration, we unbundle the three focal constructs to integrate and clarify their component …