Digital Commons Network^™

Investigating The Reversibility Of Protein Adsorption On Gold Nanoparticles And The Role Of Free Thiol, Awosiji Olatunde Awotunde

Theses and Dissertations

Protein-gold nanoparticle (AuNP) bioconjugates have many potential applications in nanomedicine. A thorough understanding of the interaction between the protein and AuNP is critical to engineering these functional bioconjugates with desirable properties. In this work, we investigate the role of free thiols presented by the protein on the stability of the protein-AuNP conjugate. Human serum albumin (HSA) was modified with 2-iminothiolane (Traut’s reagent) to introduce additional thiols on the protein surface, and three variants of HSA were synthesized to present 1, 5, and 20 free thiols by controlling the molar excess of the chemical modifier. Protein exchange studies on AuNPs were …

June 26, 2020: Covid-19 Update And Safe Return To Campus Update Vol. 6, Kettering University

Communication Updates

Coordinated by our CVPrepare Task Force, teams from across the University have been working for over two months researching, identifying, and implementing safety procedures to enable our safe return to campus on July 13.

These steps, guidelines, and processes are outlined in our Playbook for Our Safe Return, which is now online at the University’s website at kettering.edu/return2campus. In addition to a downloadable version, sections can be searched to easily find relevant and important information

Workshop #6: Psychological Adjustment For The 2020 Hurricane Season During Covid-19 Pandemic: After-Action Report (Aar), Converge Nsf Working Group, Joshua G. Behr, Wie Yusuf, Jennifer Marshall, Elizabeth Dunn

Presentations, Lectures, Posters, Reports

Participants in the CONVERGE COVID-19 Working Group’s Workshop #6 on Psychological Adjustment breakout sessions discussed the critical threats to psychological health and well-being facing shelter staff, volunteers, and clients in the 2020 hurricane season.

It is widely recognized that people are approaching the 2020 hurricane season under unusual levels of stress. The COVID-19 pandemic has created chronic stressors that individuals are already managing in addition to addressing new acute fears like –will I get sick if I go to a shelter? These new and exacerbated stressors are a risk for higher levels of burnout, compassion fatigue, and ill-being. …

Synthesizing Modified Polymeric Nanoparticles For Biofilm Inhibition, Antibiotic Encapsulation, And Specific Targeting Against Pseudomonas Aeruginosa, Logan Schnorbus

Theses and Dissertations

Widespread usage of antibiotics is a growing concern due to antibiotic resistance development in bacteria. This is due to common use of antibiotics in agricultural, livestock, and clinical usage. Antibiotic resistance is developing at a rate in which it is outpacing new drugs on the market. New strategies in drug development are necessary to combat the increasing resistance. We designed several motifs of sugar-modified nanoparticles to inhibit the biofilm formation of Pseudomonas aeruginosa.

P. aeruginosa is an opportunistic pathogenic bacterium that is responsible for common life-threatening infection in hospitals. This gram-negative opportunistic pathogenic bacterium infects hosts with compromised immune systems …

Racial And Socioeconomic Disparities In Healthcare- And Community-Associated Clostridium Difficile Infection: A Rapid Review, Rutvin Kyada, Amy Leader, Drph, Mph, Rosemary Frasso, Phd, Cph

Master of Public Health Capstone Presentations

Clostridium difficile infection (CDI) is a major cause of morbidity and mortality in the United States and has been termed by the Centers for Disease Control and Prevention as an “urgent threat” in antimicrobial resistance. Traditionally a major healthcare-associated infection, CDIs have become increasingly common in community settings as well. As usage of healthcare services and certain medications are common risk factors for CDI, an individual’s access to such services can impact the CDI burden they experience. Identifying and addressing such disparities is crucial for an equitable healthcare system, but there is a general dearth of literature reviewing sociodemographic disparities …

Reprapable Automated Open Source Bag Valve Mask-Based Ventilator, Aliaksei Petsiuk, Nagendra Gautam Tanikella, Samantha C. Dertinger, Adam Pringle, Shane Oberloier, Joshua M. Pearce

Michigan Tech Publications, Part 1

This study describes the development of an automated bag valve mask (BVM) compression system, which, during acute shortages and supply chain disruptions can serve as a temporary emergency ventilator. The resuscitation system is based on the Arduino controller with a real-time operating system installed on a largely RepRap 3-D printable parametric component-based structure. The cost of the system is under $170, which makes it affordable for replication by makers around the world. The device provides a controlled breathing mode with tidal volumes from 100 to 800 milliliters, breathing rates from 5 to 40 breaths/minute, and inspiratory-to-expiratory ratio from 1:1 to …

Vaccine Procurement In A Time-Indexed Model With Quantity-Based Discounts, Joseph N. Yeiter

Theses

Vaccination is hailed as one of the greatest scientific achievements of the past century. However, affordability and accessibility prevent low-income countries from realizing the full benefit of immunization. This study applies operations research methods to a hypothetically coordinated global vaccine market with quantity-based discounts to minimize procurement and holding costs while ensuring investment recovery of manufacturers. The effects on affordability of fixed-costs, holding costs, cold-chain capacity, and tender length (i.e. the maximum number of years ahead markets are willing to order supply from producers) are analyzed for each income-based segment of the global market. Experimental results show that increases in …

2020-06-25_Michiganoverview_Covidsymptoms, Michigan Department Of Health & Human Services

Michigan COVID-19 Syndromic Surveillance System Data

The Syndromic Surveillance System Data report was obtained from Michigan's COVID-19 dashboard, from under the link "​Coronavirus Symptoms": https://www.michigan.gov/coronavirus. Data was compiled by the Michigan Department of Health and Human Services (MDHHS). The information was updated daily.

Syndromic Surveillance System data is obtained from approximately 183 facilities participating in syndromic surveillance (132 Emergency Departments (ED), 51 Urgent Cares) across the State of Michigan. The reporting is based on individuals who present at EDs and may not be representative of the overall population. The coronavirus-like symptoms query searches visit free-text (chief complaint, triage notes, and clinical impression) for fever or …

Covid-19: The New Mexico Experience And Background, Matthew Wharton, Marisa Su, Meghan Brett, Jon Femling

Western Journal of Orthopaedics

Introduction: The novel Coronavirus Disease 19 (COVID-19) identified in December 2019 and the associated pandemic has temporarily changed the way that we conduct medicine, allocate resources, and educate resident and fellows. The present investigation is a summary of The University of New Mexico clinical experience managing the sudden surge of critical patients, personal protective equipment management (PPE), and resident education. Methods: We evaluated and analyzed the data related to the COVID-19 pandemic patient load and management in New Mexico, the formation of teams to protect the providers from simultaneous exposure, the resident experience, the PPE management, and the change in …

Jerry B. Wright, Petitioner/Appellant V. Utah Labor Commission, Brent Brown Chevrolet & Buick And/Or Worker's Compensation Fund, Respondents/Appellees. : Brief Of Appellant, Utah Court Of Appeals

Utah Court of Appeals Briefs (2020 –)

Appeal from the Labor Commission of Utah

Matthew J. Black (#8283) Attorney for Respondents/ Appellees Utah Labor Commission Christopher T. Hill Attorney for Respondent

Michael Gary Belnap (#3635) Virginius Dabney (#0795) Stony V. Olsen (#09287) Attorneys for Petitioner/ Appellant Jerry B. Wright

Research With Older People In A World With Covid-19: Identification Of Current And Future Priorities, Challenges And Opportunities, Sarah J. Richardson, Camille B. Carroll, Jacqueline Close, Adam L. Gordon, J O'Brien, Terence J. Quinn, Lynn Rochester, Avan A. Sayer, Susan D. Shenkin, Der Velde N Van, Jean Woo, Miles D. Witham

Peninsula Medical School

Older people are disproportionately affected by the COVID-19 pandemic, which has had a profound impact on research as well as clinical service delivery. This commentary identifies key challenges and opportunities in continuing to conduct research with and for older people, both during and after the current pandemic. It shares opinions from responders to an international survey, a range of academic authors and opinions from specialist societies. Priorities in COVID-19 research include its specific presentation in older people, consequences for physical, cognitive and psychological health, treatments and vaccines, rehabilitation, supporting care homes more effectively, the impact of social distancing, lockdown policies …

Lysophosphatidylcholine Acyltransferase 2 (Lpcat2) Co-Localises With Tlr4 And Regulates Macrophage Inflammatory Gene Expression In Response To Lps, W. Abate, H. Alrammah, M. Kiernan, A. J. Tonks, S. K. Jackson

School of Biomedical Sciences

Despite extensive investigations, an efective treatment for sepsis remains elusive and a better understanding of the infammatory response to infection is required to identify potential new targets for therapy. In this study we have used RNAi technology to show, for the frst time, that the inducible lysophosphatidylcholine acyltransferase 2 (LPCAT2) plays a key role in macrophage infammatory gene expression in response to stimulation with bacterial ligands. Using siRNA- or shRNA-mediated knockdown, we demonstrate that, in contrast to the constitutive LPCAT1, LPCAT2 is required for macrophage cytokine gene expression and release in response to TLR4 and TLR2 ligand stimulation but not …

We Are...Marshall, June 24, 2020, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

2020-06-24_Michiganoverview_Covidsymptoms, Michigan Department Of Health & Human Services

Michigan COVID-19 Syndromic Surveillance System Data

The Syndromic Surveillance System Data report was obtained from Michigan's COVID-19 dashboard, from under the link "​Coronavirus Symptoms": https://www.michigan.gov/coronavirus. Data was compiled by the Michigan Department of Health and Human Services (MDHHS). The information was updated daily.

Syndromic Surveillance System data is obtained from approximately 183 facilities participating in syndromic surveillance (132 Emergency Departments (ED), 51 Urgent Cares) across the State of Michigan. The reporting is based on individuals who present at EDs and may not be representative of the overall population. The coronavirus-like symptoms query searches visit free-text (chief complaint, triage notes, and clinical impression) for fever or …

Innovations, Challenges, And Minimal Information For Standardization Of Humanized Mice., Renata Stripecke, Christian Münz, Jan Jacob Schuringa, Karl-Dimiter Bissig, Brian Soper, Terrence Meeham, Li-Chin Yao, James P Di Santo, Michael Brehm, Estefania Rodriguez, Anja Kathrin Wege, Dominique Bonnet, Silvia Guionaud, Kristina E Howard, Scott Kitchen, Florian Klein, Kourosh Saeb-Parsy, Johannes Sam, Amar Deep Sharma, Andreas Trumpp, Livio Trusolino, Carol J Bult, Leonard D. Shultz

Faculty Research 2020

Mice xenotransplanted with human cells and/or expressing human gene products (also known as "humanized mice") recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human-specific physiology and pathologies. Humanized mice have advanced toward mainstream preclinical models and are now at the forefront of biomedical research. Here, we considered innovations and challenges regarding the reconstitution of human immunity and human tissues, modeling of human infections and cancer, and the use of humanized mice for testing drugs or regenerative therapy products. As the number of publications exploring …

Workshop #5: Workforce: Evacuations, Shelter Staffing, Workforce Structure, Capacity, Ppe, And Telemedicine: After-Action Report (Aar), Converge Nsf Working Group, Joshua G. Behr, Wie Yusuf, Jennifer Marshall, Elizabeth Dunn

Presentations, Lectures, Posters, Reports

Participants in the breakout sessions for the CONVERGE COVID-19 Working Group’s Workshop 5 (Workforce) identified several issues, including unique staffing challenges for this compound event, needed training revisions to address shelter protocols specific to COVID-19, additional staffing needs based on the unusual use of congregate and non-congregate shelters to address COVID-19 risks, and the increased need for ancillary services for staff and volunteers during and after an event.

Ensuring shelter operations are maintained despite COVID-19 will require adequate staffing. Layoffs, furloughs and hiring freezes have affected base employee numbers, where many jurisdictions rely on county and city employees, along with …

The Mbeya Antimicrobial Stewardship Team: Implementing Antimicrobial Stewardship At A Zonal-Level Hospital In Southern Tanzania, Jeffrey W. Hall, Jeannette Bouchard, P Brandon Bookstaver, Matthew S. Haldeman, Peter Kishimbo, Godlove Mbwanji, Issakwisa Mwakyula, Davance Mwasomola, Megan Seddon, Mark Shaffer, Stephanie C. Shealy, Anthony Nsojo

Faculty Publications

In 2017, Mbeya Zonal Referral Hospital (MZRH) and the University of South Carolina (UofSC) agreed to collaboratively strengthen antimicrobial prescribing in the southern highlands of Tanzania and train a new generation of clinicians in responsible antimicrobial use. Key stakeholders and participants were identified and the Mbeya Antimicrobial Stewardship Team (MAST) was created. The team identified assets brought by the collaborators, and four investigations of baseline needs were developed. These investigations included (a) a baseline clinician survey regarding antimicrobial resistance and stewardship, (b) a serial chart review of inpatient antimicrobial prescribing practices, (c) an investigation of antimicrobial resistance rates using existing …

Evaluation Of The Biofire Filmarray Pneumonia Panel For Detection Of Viral And Bacterial Pathogens In Lower Respiratory Tract Specimens In The Setting Of A Tertiary Care Academic Medical Center, Daniel M Webber, Meghan A Wallace, C A Burnham, Neil W Anderson

Open Access Publications

Our objective was to evaluate the diagnostic yield and accuracy of the BioFire FilmArray pneumonia panel (BFPP) for identification of pathogens in lower respiratory tract specimens (

Coronavirus (Covid-19) Fulminant Myopericarditis And Acute Respiratory Distress Syndrome (Ards) In A Middle-Aged Male Patient, Victor Guardiola

All Publications

Myopericarditis remains a prominent infectious inflammatory disorder throughout a patient’s lifetime. Moreover, viral pathogens have been proven to be the leading contributors to myopericarditis in the pediatric and adult populations. Despite the current comprehensive knowledge of myocardial injury in viral and post-viral myopericarditis, the cellular and molecular mechanisms of SARS-CoV-2-induced myopericarditis are poorly understood. This report presents a case of coronavirus (COVID-19) fulminant myopericarditis and acute respiratory distress syndrome (ARDS) in a middle-aged male patient: a 51-year-old man with a history of hypertension who arrived to the emergency department with a dry cough, fatigue, dyspnea, and a fever. A real-time …

2020-06-23_Michiganoverview_Covidsymptoms, Michigan Department Of Health & Human Services

Michigan COVID-19 Syndromic Surveillance System Data

The Syndromic Surveillance System Data report was obtained from Michigan's COVID-19 dashboard, from under the link "​Coronavirus Symptoms": https://www.michigan.gov/coronavirus. Data was compiled by the Michigan Department of Health and Human Services (MDHHS). The information was updated daily.

Syndromic Surveillance System data is obtained from approximately 183 facilities participating in syndromic surveillance (132 Emergency Departments (ED), 51 Urgent Cares) across the State of Michigan. The reporting is based on individuals who present at EDs and may not be representative of the overall population. The coronavirus-like symptoms query searches visit free-text (chief complaint, triage notes, and clinical impression) for fever or …

2020-06-22/23 Daily Unm Global Health Covid-19 Briefing, Christophe G. Lambert, Shawn Stoicu, Ingrid Hendrix, Lori D. Sloane, Mari Anixter, Anastasiya Nestsiarovich, Praveen Kumar, Nicolas Lauve, Randy Ko, Jenny Situ, Alexendra Yingling, Tudor I. Oprea, Kristine Tollestrup, Orrin Myers, Douglas J. Perkins

HSC Covid 19 Briefings

Executive Summary:

NM Highlights: Balloon Fiesta postponed. Robot to sanitize ABQ International Sunport. NMSU plan for fall 2020. NM public schools reopening plan. Fewer hospitalizations for COVID-19. NM case update. US Highlights: Visa restrictions. International Highlights: Second wave in South Korea. Epidemiology: Lockdown can suppress COVID-19. Case fatality rate associated with incidence. Predictors of ICU care and ventilators. Anxiety and depression from COVID-19. Asymptomatic patients as source of infection. Heterogeneous populations affect herd immunity. Healthcare Policy Recommendations: New FDA guidance on clinical trials conduct. Practice Guidelines: The guidelines are provided on COVID-19 diagnostics (Infectious Diseases Society of America), respiratory support …

Transcriptomic Analysis Of Extracellular Rna Governed By The Endocytic Adaptor Protein Cin1 Of Cryptococcus Deneoformans, Muxing Liu, Zhengguang Zhang, Chen Ding, Tuo Wang, Ben Kelly, Ping Wang

Faculty Publications

© Copyright © 2020 Liu, Zhang, Ding, Wang, Kelly and Wang. Membrane vesicles are considered virulence cargoes as they carry capsular and melanin components whose secretory transport is critical for the virulence of the human fungal pathogen Cryptococcus species. However, other components of the vesicles and their function in the growth and virulence of the fungus remain unclear. We have previously found that the cryptococcal intersectin protein Cin1 governs a unique Cin1-Wsp1-Cdc42 endocytic pathway required for intracellular transport and virulence. Using RNA sequencing, we compared the profiles of extracellular RNA (exRNA), including microRNA (miRNA), small interference RNA (siRNA), long noncoding …

Treating And/Or Preventing Insulin-Resistance Related Weight Gain, Robert Vito Farese, Mini Paliyath Sajan

USF Patents

Provided herein are methods of preventing or reducing weight gain in a subject in need thereof by administering 2-acetylcyclopentane-1,3-dione (ACPD).

Macronutrient Intake And Simulated Infection Threat Independently Affect Life History Traits Of Male Decorated Crickets, Kristin Duffield, Kylie J. Hampton, James Rapkin, John Hunt, Scott K. Sakaluk, Ben M. Sadd, Thomas M. Houslay

Faculty Publications – Biological Sciences

Nutritional geometry has advanced our understanding of how macronutrients (e.g.,proteins and carbohydrates) influence the expression of life history traits and theircorresponding trade-offs. For example, recent work has revealed that reproduc-tion and immune function in male decorated crickets are optimized at very differentprotein:carbohydrate (P:C) dietary ratios. However, it is unclear how an individual'smacronutrient intake interacts with its perceived infection status to determine in-vestment in reproduction or other key life history traits. Here, we employed a fullyfactorial design in which calling effort and immune function were quantified formale crickets fed either diets previously demonstrated to maximize calling effort(P:C = 1:8) or immune …

Class Notes/Depaul Pride

DePaul Magazine

Brief updates on DePaul alumni.

Synthesis Of Gold Nanoparticles By Using Batch Method And Study Its Antibacterial Activity, Mahmood Mohammed Ali, Firas Fadhel Ali, Ahmed Mohammed Et Al.

Baghdad Science Journal

The research involves preparing gold nanoparticles (AuNPs) and studying the factors that influence the shape, sizes and distribution ratio of the prepared particles according to Turkevich method. These factors include (reaction temperature, initial heating, concentration of gold ions, concentration and quantity of added citrate, reaction time and order of reactant addition). Gold nanoparticles prepared were characterized by the following measurements: UV-Visible spectroscopy, X-ray diffraction and scanning electron microscopy. The average size of gold nanoparticles was formed in the range (20 -35) nm. The amount of added citrate was changed and studied. In addition, the concentration of added gold ions was …

2020-06-22_Michiganoverview_Covidsymptoms, Michigan Department Of Health & Human Services

Michigan COVID-19 Syndromic Surveillance System Data

The Syndromic Surveillance System Data report was obtained from Michigan's COVID-19 dashboard, from under the link "​Coronavirus Symptoms": https://www.michigan.gov/coronavirus. Data was compiled by the Michigan Department of Health and Human Services (MDHHS). The information was updated daily.

Syndromic Surveillance System data is obtained from approximately 183 facilities participating in syndromic surveillance (132 Emergency Departments (ED), 51 Urgent Cares) across the State of Michigan. The reporting is based on individuals who present at EDs and may not be representative of the overall population. The coronavirus-like symptoms query searches visit free-text (chief complaint, triage notes, and clinical impression) for fever or …

Investigating The Dynamics Of Elk Population Size And Body Mass In A Seasonal Environment Using A Mechanistic Integral Projection Model, Shelly Lachish, Ellen E. Brandell, Meggan E. Craft, Andrew P. Dobson, Peter J. Hudson, Daniel R. Macnulty, Tim Coulson

Wildland Resources Faculty Publications

Environmentally mediated changes in body size often underlie population responses to environmental change, yet this is not a universal phenomenon. Understanding when phenotypic change underlies population responses to environmental change is important for obtaining insights and robust predictions of population dynamics in a changing world. We develop a dynamic integral projection model that mechanistically links environmental conditions to demographic rates and phenotypic traits (body size) via changes in resource availability and individual energetics. We apply the model to the northern Yellowstone elk population and explore population responses to changing patterns of seasonality, incorporating the interdependence of growth, demography, and density-dependent …

Veterinarians’ Attitudes And Practices Regarding Opioid-Related Vet Shopping In Tri-State Appalachian Counties: An Exploratory Study, Falguni C. Patel, Jeffrey A. Raines, Richard W. Kim, Karen Gruszynski, Robert E. Davis, Manoj Sharma, Gilbert Patterson, Jason W. Johnson, Vinayak K. Nahar

Environmental & Global Health Faculty Research

Background: The opioid crisis continues to grow in the United States with 46,700 drug overdose deaths due to opioids in 2017 alone. Vet shopping, the practice of soliciting veterinarians for prescription medications, has been receiving national media attention in recent years. A 2014 review of Prescription Monitoring Drug Programs found less than 10 veterinary shoppers nationwide. Still much is unknown about the role of vet shopping and the opioid crisis. This study sought to understand the practice of vet shopping through the eyes of veterinarians practicing in Appalachian counties within the states of Kentucky, Tennessee, and Virginia, United States. Results: …

Unique Case Of Gastroenteritis Presenting As Pneumatosis Intestinalis With Emphysematous Gastritis And Portal Vein Gas: Do Not Take Gastroenteritis Lightly., Asif Mehmood, Rajesh Essrani Md, Swetha Parvataneni, Umair Iqbal

Department of Medicine

Pneumatosis intestinalis (PI) consists of multiple, thin, gas-filled cysts in the wall of the gastrointestinal (GI) tract. It is an uncommon entity that can involve any gastrointestinal site from the stomach to the rectum. Isolated stomach involvement is rare. PI can represent a broad spectrum of diseases with variable prognoses. We present the case of a patient who was admitted with gastroenteritis-like symptoms. He remained hemodynamically stable, and on further imaging with contrast-enhanced computed tomography of the abdomen and pelvis, air was found in the portal vein and gastric wall, with minimal thickening of the proximal small bowel concerning for …