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Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakask K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan Jan 2021

Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakask K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV) infection to a pregnant woman can be vertically transmitted to the fetus via the placenta leading to Congenital Zika syndrome. This is characterized by microcephaly, retinal defects, and intrauterine growth retardation. ZIKV induces placental trophoblast apoptosis leading to severe abnormalities in the growth and development of the fetus. However, the molecular mechanism behind ZIKV-induced apoptosis in placental trophoblasts remains unclear. We hypothesize that ZIKV infection induces endoplasmic reticulum (ER) stress in the trophoblasts, and sustained ER stress results in apoptosis. HTR-8 (HTR-8/SVneo), a human normal immortalized trophoblast cell and human choriocarcinoma-derived cell lines (JEG-3 and JAR) were …


Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle Jan 2021

Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle

Karl Reinhard Publications

Intestinal contents were sampled from a spontaneously enhanced mummy from the Sepulcher of the Priests of the Piraino Mother Church in the Province of Messina, Sicily. This adult male mummy, Piraino 1, is an unidentified religious dignitary dating from the late-18th to mid-19th centuries. Immunological and molecular diagnostics were used to test for common and clinically significant parasites. A morphological diagnosis of Trichuris trichiura (whipworm) was confirmed genetically. A previously undetected Enterobius vermicularis (pinworm) infection was also identified genetically. These data indicate that the Piraino 1 individual was simultaneously infected with multiple intestinal parasite species indicative of poor hygiene. This …


Biodiversity Of The Coccidia (Apicomplexa: Conoidasida) In Vertebrates: What We Know, What We Do Not Know, And What Needs To Be Done, Donald W. Duszynski Jan 2021

Biodiversity Of The Coccidia (Apicomplexa: Conoidasida) In Vertebrates: What We Know, What We Do Not Know, And What Needs To Be Done, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Over the last two decades my colleagues and I have assembled the literature on a good percentage of most of the coccidians (Conoidasida) known, to date, to parasitise: Amphibia, four major lineages of Reptilia (Amphisbaenia, Chelonia, Crocodylia, Serpentes), and seven major orders in the Mammalia (Carnivora, Chiroptera, Lagomorpha, Insectivora, Marsupialia, Primates, Scandentia). These vertebrates, combined, comprise about 15,225 species; only about 899 (5.8%) of them have been surveyed for coccidia and 1,946 apicomplexan valid species names or other forms are recorded in the literature. Based on these compilations and other factors, I extrapolated that there yet may be an additional …


A Study On The Pathological Effects Of Trypanorhyncha Cestodes In Dusky Groupers Epinephelus Marginatus From The Canary Islands, Carolina De Sales-Ribeiro, Miguel A. Rivero, Antonio Fernández, Natalia García-Álvarez, Jorge Francisco González, Oscar Quesada-Canales, María José Caballero Jan 2021

A Study On The Pathological Effects Of Trypanorhyncha Cestodes In Dusky Groupers Epinephelus Marginatus From The Canary Islands, Carolina De Sales-Ribeiro, Miguel A. Rivero, Antonio Fernández, Natalia García-Álvarez, Jorge Francisco González, Oscar Quesada-Canales, María José Caballero

Harold W. Manter Laboratory of Parasitology: Library Materials

Trypanorhyncha are cestodes commonly infecting marine fish. Numerous studies have detailed the biology of Trypanorhyncha species, but information on the pathological changes produced by these parasites is limited. Dusky groupers are keystone species necessary for the preservation of several marine ecosystems. Considering their vulnerable state of conservation and the efforts being made to culture them, identification of the effects caused by Trypanorhyncha is vital. Here, we aimed to determine the prevalence and pathological changes produced by Trypanorhyncha in dusky groupers from the Canary Islands. The prevalence of trypanorhynch plerocerci was 96%. Grossly, in the abdominal cavity, there were numerous larvae-filled …


Human Body-Fluid Proteome: Quantitative Profiling And Computational Prediction, Lan Huang, Dan Shao, Yan Wang, Xueteng Cui, Yufei Li, Qian Chen, Juan Cui Jan 2021

Human Body-Fluid Proteome: Quantitative Profiling And Computational Prediction, Lan Huang, Dan Shao, Yan Wang, Xueteng Cui, Yufei Li, Qian Chen, Juan Cui

School of Computing: Faculty Publications

Empowered by the advancement of high-throughput bio technologies, recent research on body-fluid proteomes has led to the discoveries of numerous novel disease biomarkers and therapeutic drugs. In the meantime, a tremendous progress in disclosing the body-f luid proteomes was made, resulting in a collection of over 15 000 different proteins detected in major human body fluids. However, common challenges remain with current proteomics technologies about how to effectively handle the large variety of protein modifications in those fluids. To this end, computational effort utilizing statistical and machine-learning approaches has shown early successes in identifying biomarker proteins in specific human diseases. …


At A Covid Crossroads : Public Health, Patient Privacy, And Health Information Confidentiality, Stacey A. Tovino Jan 2021

At A Covid Crossroads : Public Health, Patient Privacy, And Health Information Confidentiality, Stacey A. Tovino

Faculty Articles

This essay summarizes and assesses the various bulletins, guidance documents, and notices of enforcement discretion released by the federal Department of Health and Human Services regarding the application of the HIPAA Privacy Rule to the COVID-19 pandemic. Among other topics and actions, these authorities address the application of the HIPAA Privacy Rule to the use and disclosure of protected health information for public health activities, waive the application of certain HIPAA Privacy Rule requirements during the COVID-19 pandemic, and announce enforcement discretion regarding certain covered entities’ non-compliance with particular provisions within the HIPAA Privacy Rule. These authorities overwhelmingly, and appropriately, …


Analysis Of Designs Used In Monitoring Crop Growth Based On Remote Sensing Methods, Cristina Teodora Dobrota, Rahela Carpa, Anca Butiuc-Keul Jan 2021

Analysis Of Designs Used In Monitoring Crop Growth Based On Remote Sensing Methods, Cristina Teodora Dobrota, Rahela Carpa, Anca Butiuc-Keul

Turkish Journal of Agriculture and Forestry

Choosing appropriate designs and methods for monitoring crop growth is a challenging process of major importance. Remote sensing from space and manned or unmanned airborne operations are used to measure crop reflectance and a wide variety of other agricultural parameters. While some experiments use only a few, specific methods and designs and organize the results in lists of evidence, other experiments use a wider range of techniques to create a more credible and comprehensive assessment of crop yield. Particular situations related to the available resources in terms of data collection and expertise in addition to the intended use of the …


Application Of Optimal Control Theory To A Malaria Model, Cassidy Hill Jan 2021

Application Of Optimal Control Theory To A Malaria Model, Cassidy Hill

Murray State Theses and Dissertations

With malaria still prevalent and considered to be one of the most devastating infectious diseases in the world, many scientific efforts have been made to reduce its impact. One such effort includes the construction of mathematical models. Mathematical models can be used to analyze malaria transmission dynamics in the human population. The development of these models allows researchers to consider the control measures necessary to reduce the prevalence of malaria infection and possibly eliminate it.

The model presented in this thesis will provide the relationship of female Anopheles mosquitoes and insecticide treated paint acting as the control. A deterministic system …


Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett Jan 2021

Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett

Murray State Theses and Dissertations

Over the past four decades, emergent fungal diseases have been the most devastating relative to species declines and extinctions. While most research has focused on fungal diseases affecting amphibians and bats, less has focused on diseases like snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola (Oo). SFD was first described in 2006 in North America within a Timber rattlesnake (Crotalus horridus) population in New Hampshire. Since then SFD has been documented in 19 US states, one US territory (Puerto Rico), and Europe. SFD causes high mortality in some species, including the Eastern Massasauga rattlesnakes (Sistrurus …


Perceptions Of Covid-19, Information Seeking Behavior, And Political Orientation, Bailey Dodd, Sean Rife Jan 2021

Perceptions Of Covid-19, Information Seeking Behavior, And Political Orientation, Bailey Dodd, Sean Rife

Murray State Theses and Dissertations

COVID-19 was declared an international pandemic in March 2020 and infected millions of people globally in less than six months. Research suggests that a large amount of both accurate and misinformation exists on the pandemic, and it would be expected that an individual’s political orientation would influence their perception of the virus and the information that they endorse. Based on past research, it would be expected that both liberals and conservatives would be equally concerned about the COVID-19 pandemic and endorse similar measures. However, Republicans downplayed the pandemic and were more likely to consider it a hoax, while Democrats exaggerated …


Examining Psychological Distress In Parents Of Children With Food Allergies And Asthma During The Covid-19 Pandemic, Kelsey L. Hill Jan 2021

Examining Psychological Distress In Parents Of Children With Food Allergies And Asthma During The Covid-19 Pandemic, Kelsey L. Hill

Master's Theses and Doctoral Dissertations

The COVID-19 pandemic in the United States (U.S.) was anticipated to have a tremendous impact on mental health, leading to heightened anxiety, depression, and post-traumatic stress disorder. The current study examined the psychological impact of the COVID-19 pandemic in parents of children with food allergies and asthma compared to parents of healthy children, examined associations between disease-specific measures and broad measures of anxiety, and explored moderating variables impacting the relationship between COVID-19-related stressors and psychological outcomes. A national sample of 265 parents completed an online survey between April and June 2020. Parents of children with food allergies and asthma experienced …


Comparison Of Voluntary Versus Mandatory Vaccine Discussions In Online Health Communities: A Text Analytics Approach, Massara Alazazi, Bin Wang Jan 2021

Comparison Of Voluntary Versus Mandatory Vaccine Discussions In Online Health Communities: A Text Analytics Approach, Massara Alazazi, Bin Wang

Information Systems Faculty Publications

Vaccines are vital health interventions. However, they are controversial and some people support them while others reject them. Social media discussion and big data are a rich source to understand people’s insights about different vaccines and the related topics that concern most of them. This study aims to explore the online discussions about mandatory and voluntary vaccines using text analysis techniques. Reddit social platform is popular in online health discussion and thus data from Reddit is analyzed. The results show that different aspects are discussed for different types of vaccines. The discussion of mandatory vaccines is more interactive and is …


"Who Can Help Me?'': Knowledge Infused Matching Of Support Seekers And Support Providers During Covid-19 On Reddit, Manas Gaur, Kaushik Roy, Aditya Sharma, Biplav Srivastava, Amit Sheth Jan 2021

"Who Can Help Me?'': Knowledge Infused Matching Of Support Seekers And Support Providers During Covid-19 On Reddit, Manas Gaur, Kaushik Roy, Aditya Sharma, Biplav Srivastava, Amit Sheth

Publications

During the ongoing COVID-19 crisis, subreddits on Reddit, such as r/Coronavirus saw a rapid growth in user's requests for help (support seekers - SSs) including individuals with varying professions and experiences with diverse perspectives on care (support providers - SPs). Currently, knowledgeable human moderators match an SS with a user with relevant experience, i.e, an SP on these subreddits. This unscalable process defers timely care. We present a medical knowledge-infused approach to efficient matching of SS and SPs validated by experts for the users affected by anxiety and depression, in the context of with COVID-19. After matching, each SP to …


Short-Range Forecasting Of Covid-19 During Early Onset At County, Health District, And State Geographic Levels Using Seven Methods: Comparative Forecasting Study, Christopher Lynch, Ross Gore Jan 2021

Short-Range Forecasting Of Covid-19 During Early Onset At County, Health District, And State Geographic Levels Using Seven Methods: Comparative Forecasting Study, Christopher Lynch, Ross Gore

VMASC Publications

BACKGROUND:

Forecasting methods rely on trends and averages of prior observations to forecast COVID-19 case counts. COVID-19 forecasts have received much media attention, and numerous platforms have been created to inform the public. However, forecasting effectiveness varies by geographic scope and is affected by changing assumptions in behaviors and preventative measures in response to the pandemic. Due to time requirements for developing a COVID-19 vaccine, evidence is needed to inform short-term forecasting method selection at county, health district, and state levels.

OBJECTIVE:

COVID-19 forecasts keep the public informed and contribute to public policy. As such, proper understanding of forecasting purposes …


Spatio-Temporal Modelling Of Tick Life-Stage Count Data With Spatially Varying Coefficients, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff Jan 2021

Spatio-Temporal Modelling Of Tick Life-Stage Count Data With Spatially Varying Coefficients, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff

Biological Sciences Faculty Publications

There is a vast amount of geo-referenced data in many fields of study including ecological studies. Geo-referencing is usually by point referencing; that is, latitudes and longitudes or by areal referencing, which includes districts, counties, states, provinces and other administrative units. The availability of large geo-referenced datasets for modelling has necessitated the development and application of spatial statistical methods. However, spatial varying coefficients models exploring the abundance of tick counts remain limited. In this study we used data that was collected and prepared by researchers in the Department of Biological Sciences from the Old Dominion University, Virginia, USA. We modelled …


Covid-19 And Regulating Vaccines, Dorit R. Reiss Jan 2021

Covid-19 And Regulating Vaccines, Dorit R. Reiss

Faculty Scholarship

No abstract provided.


Utility Of Repeat Testing For Covid-19: Laboratory Stewardship When The Stakes Are High, Lindsey M. Rearigh, Angela L. Hewlett, Paul D. Fey, M. Jana Broadhurst, David Brett-Major, Mark Rupp, Trevor Van Schooneveld Jan 2021

Utility Of Repeat Testing For Covid-19: Laboratory Stewardship When The Stakes Are High, Lindsey M. Rearigh, Angela L. Hewlett, Paul D. Fey, M. Jana Broadhurst, David Brett-Major, Mark Rupp, Trevor Van Schooneveld

Journal Articles: Epidemiology

As the coronavirus disease 2019 (COVID-19) continues to circulate, testing strategies are of the utmost importance. Given national shortages of testing supplies, personal protective equipment, and other hospital resources, diagnostic stewardship is necessary to aid in resource management. We report the low utility of serial testing in a low-prevalence setting.


Recommended Reporting Items For Epidemic Forecasting And Prediction Research: The Epiforge 2020 Guidelines, Simon Pollett, Michael A. Johansson, Nicholas G. Reich, David Brett-Major, Sara Y. Del Valle, Srinivasan Venkatramanan, Rachel Lowe, Travis Porco, Irina Maljkovic Berry, Alina Deshpande, Moritz U. G. Kraemer, David L. Blazes, Wirichada Pan-Ngum, Alessandro Vespigiani, Suzanne E. Mate, Sheetal P. Silal, Sasikiran Kandula, Rachel Sippy, Talia M. Quandelacy, Jeffrey J. Morgan, Jacob Ball, Lindsay C. Morton, Benjamin M. Althouse, Julie Pavlin, Wilbert Van Panhuis, Steven Riley, Matthew Biggerstaff, Cecile Viboud, Oliver Brady, Caitlin Rivers Jan 2021

Recommended Reporting Items For Epidemic Forecasting And Prediction Research: The Epiforge 2020 Guidelines, Simon Pollett, Michael A. Johansson, Nicholas G. Reich, David Brett-Major, Sara Y. Del Valle, Srinivasan Venkatramanan, Rachel Lowe, Travis Porco, Irina Maljkovic Berry, Alina Deshpande, Moritz U. G. Kraemer, David L. Blazes, Wirichada Pan-Ngum, Alessandro Vespigiani, Suzanne E. Mate, Sheetal P. Silal, Sasikiran Kandula, Rachel Sippy, Talia M. Quandelacy, Jeffrey J. Morgan, Jacob Ball, Lindsay C. Morton, Benjamin M. Althouse, Julie Pavlin, Wilbert Van Panhuis, Steven Riley, Matthew Biggerstaff, Cecile Viboud, Oliver Brady, Caitlin Rivers

Journal Articles: Epidemiology

BACKGROUND: The importance of infectious disease epidemic forecasting and prediction research is underscored by decades of communicable disease outbreaks, including COVID-19. Unlike other fields of medical research, such as clinical trials and systematic reviews, no reporting guidelines exist for reporting epidemic forecasting and prediction research despite their utility. We therefore developed the EPIFORGE checklist, a guideline for standardized reporting of epidemic forecasting research.

METHODS AND FINDINGS: We developed this checklist using a best-practice process for development of reporting guidelines, involving a Delphi process and broad consultation with an international panel of infectious disease modelers and model end users. The objectives …


Long-Term Assessment Of The Effects Of Covid-19 And Isolation Care On Survivor Disability And Anxiety, David Brett-Major, David S. Cates, James V. Lawler, Chad Vokoun, Angela L. Hewlett, Daniel W. Johnson, Elizabeth R. Schnaubelt, Michael C. Wadman, Christopher J. Kratochvil, M. Jana Broadhurst Jan 2021

Long-Term Assessment Of The Effects Of Covid-19 And Isolation Care On Survivor Disability And Anxiety, David Brett-Major, David S. Cates, James V. Lawler, Chad Vokoun, Angela L. Hewlett, Daniel W. Johnson, Elizabeth R. Schnaubelt, Michael C. Wadman, Christopher J. Kratochvil, M. Jana Broadhurst

Journal Articles: Epidemiology

We conducted an assessment of disability, anxiety, and other life impacts of COVID-19 and isolation care in a unique cohort of individuals. These included both community admissions to a university hospital as well as some of the earliest international aeromedical evacuees. Among an initial 16 COVID-19 survivors that were interviewed 6-12 months following their admission into isolation care, perception of their isolation care experience was related to their reporting of long-term consequences. However, anxiety and disability assessed with standard scores had no relationship with each other. Both capture of the isolation care experience and caution relying on single scoring systems …


Assessment Of A Program For Sars-Cov-2 Screening And Environmental Monitoring In An Urban Public School District, John Crowe, Andy T. Schnaubelt, Scott Schmidtbonne, Kathleen Angell, Julia Bai, Teresa Eske, Molly Nicklin, Catherine Pratt, Bailey White, Brodie Crotts-Hannibal, Nicholas Staffend, Vicki L. Herrera, Jeramie Cobb, Jennifer Conner, Julie Carstens, Jonell Tempero, Lori Bouda, Matthew Ray, James V. Lawler, Walter S. Campbell, John-Martin Lowe, Joshua L. Santarpia, Shannon Bartelt-Hunt, Michael R. Wiley, David Brett-Major, Cheryl Logan, M. Jana Broadhurst Jan 2021

Assessment Of A Program For Sars-Cov-2 Screening And Environmental Monitoring In An Urban Public School District, John Crowe, Andy T. Schnaubelt, Scott Schmidtbonne, Kathleen Angell, Julia Bai, Teresa Eske, Molly Nicklin, Catherine Pratt, Bailey White, Brodie Crotts-Hannibal, Nicholas Staffend, Vicki L. Herrera, Jeramie Cobb, Jennifer Conner, Julie Carstens, Jonell Tempero, Lori Bouda, Matthew Ray, James V. Lawler, Walter S. Campbell, John-Martin Lowe, Joshua L. Santarpia, Shannon Bartelt-Hunt, Michael R. Wiley, David Brett-Major, Cheryl Logan, M. Jana Broadhurst

Journal Articles: Epidemiology

Importance: Scalable programs for school-based SARS-CoV-2 testing and surveillance are needed to guide in-person learning practices and inform risk assessments in kindergarten through 12th grade settings.

Objectives: To characterize SARS-CoV-2 infections in staff and students in an urban public school setting and evaluate test-based strategies to support ongoing risk assessment and mitigation for kindergarten through 12th grade in-person learning.

Design, Setting, and Participants: This pilot quality improvement program engaged 3 schools in Omaha, Nebraska, for weekly saliva polymerase chain reaction testing of staff and students participating in in-person learning over a 5-week period from November 9 to December 11, 2020. …


The Future Of Zoonotic Risk Prediction, Colin J. Carlson, Maxwell J. Farrell, Zoe Grange, Barbara A. Han, Nardus Mollentze, Alexandra L. Phelan, Angela L. Rasmussen, Gregory F. Albery, Bernard Bett, David Brett-Major, Lily E. Cohen, Tad Dallas, Evan A. Eskew, Anna C. Fagre, Kristian M. Forbes, Rory Gibb, Sam Halabi, Charlotte C. Hammer, Rebecca Katz, Jason Kindrachuk, Renata L. Muylaert, Felicia B. Nutter, Joseph Ogola, Kevin J. Olival, Michelle Rourke, Sadie J. Ryan, Noam Ross, Stephanie N. Seifert, Tarja Sironen, Claire J. Standley, Kishana Taylor, Marietjie Venter, Paul W. Webala Jan 2021

The Future Of Zoonotic Risk Prediction, Colin J. Carlson, Maxwell J. Farrell, Zoe Grange, Barbara A. Han, Nardus Mollentze, Alexandra L. Phelan, Angela L. Rasmussen, Gregory F. Albery, Bernard Bett, David Brett-Major, Lily E. Cohen, Tad Dallas, Evan A. Eskew, Anna C. Fagre, Kristian M. Forbes, Rory Gibb, Sam Halabi, Charlotte C. Hammer, Rebecca Katz, Jason Kindrachuk, Renata L. Muylaert, Felicia B. Nutter, Joseph Ogola, Kevin J. Olival, Michelle Rourke, Sadie J. Ryan, Noam Ross, Stephanie N. Seifert, Tarja Sironen, Claire J. Standley, Kishana Taylor, Marietjie Venter, Paul W. Webala

Journal Articles: Epidemiology

In the light of the urgency raised by the COVID-19 pandemic, global investment in wildlife virology is likely to increase, and new surveillance programmes will identify hundreds of novel viruses that might someday pose a threat to humans. To support the extensive task of laboratory characterization, scientists may increasingly rely on data-driven rubrics or machine learning models that learn from known zoonoses to identify which animal pathogens could someday pose a threat to global health. We synthesize the findings of an interdisciplinary workshop on zoonotic risk technologies to answer the following questions. What are the prerequisites, in terms of open …


Multiplex Qpcr Discriminates Variants Of Concern To Enhance Global Surveillance Of Sars-Cov-2, Chantal B. F. Vogels, Mallery I. Breban, Isabel M. Ott, Tara Alpert, Mary E. Petrone, Anne E. Watkins, Chaney C. Kalinich, Rebecca Earnest, Jessica E. Rothman, Jaqueline Goes De Jesus, Ingra Morales Claro, Giulia Magalhães Ferreira, Myuki A. E. Crispim, Brazil-Uk Cadde Genomic Network, Lavanya Singh, Houriiyah Tegally, Ugochukwu J. Anyaneji, Network For Genomic Surveillance In South Africa, Emma B. Hodcroft, Christopher E. Mason, Gaurav Khullar, Jessica Metti, Joel T. Dudley, Matthew J. Mackay, Megan Nash, Jianhui Wang, Chen Liu, Pei Hui, Steven Murphy, Caleb Neal, Eva Laszlo, Marie L. Landry, Anthony Muyombwe, Randy Downing, Jafar Razeq, Tulio De Oliveira, Nuno R. Faria, Ester C. Sabino, Richard A. Neher, Joseph R. Fauver, Nathan D. Grubaugh Jan 2021

Multiplex Qpcr Discriminates Variants Of Concern To Enhance Global Surveillance Of Sars-Cov-2, Chantal B. F. Vogels, Mallery I. Breban, Isabel M. Ott, Tara Alpert, Mary E. Petrone, Anne E. Watkins, Chaney C. Kalinich, Rebecca Earnest, Jessica E. Rothman, Jaqueline Goes De Jesus, Ingra Morales Claro, Giulia Magalhães Ferreira, Myuki A. E. Crispim, Brazil-Uk Cadde Genomic Network, Lavanya Singh, Houriiyah Tegally, Ugochukwu J. Anyaneji, Network For Genomic Surveillance In South Africa, Emma B. Hodcroft, Christopher E. Mason, Gaurav Khullar, Jessica Metti, Joel T. Dudley, Matthew J. Mackay, Megan Nash, Jianhui Wang, Chen Liu, Pei Hui, Steven Murphy, Caleb Neal, Eva Laszlo, Marie L. Landry, Anthony Muyombwe, Randy Downing, Jafar Razeq, Tulio De Oliveira, Nuno R. Faria, Ester C. Sabino, Richard A. Neher, Joseph R. Fauver, Nathan D. Grubaugh

Journal Articles: Epidemiology

With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages. It was found that the B.1.1.7 (also 501Y.V1) variant, first detected in the United Kingdom, could be serendipitously detected by the Thermo Fisher TaqPath COVID-19 PCR assay because a key deletion in these viruses, spike Δ69-70, would cause a "spike gene target failure" (SGTF) result. However, a SGTF result is not definitive for B.1.1.7, and this assay cannot detect other variants of concern (VOC) that lack …


Isolation And Characterization Of A Novel Bacteriophage Wo From Allonemobius Socius Crickets In Missouri, Jonah Kupritz, John Martin, Kerstin Fischer, Kurt C. Curtis, Joseph R. Fauver, Yuefang Huang, Young-Jun Choi, Wandy L. Beatty, Makedonka Mitreva, Peter U. Fischer Jan 2021

Isolation And Characterization Of A Novel Bacteriophage Wo From Allonemobius Socius Crickets In Missouri, Jonah Kupritz, John Martin, Kerstin Fischer, Kurt C. Curtis, Joseph R. Fauver, Yuefang Huang, Young-Jun Choi, Wandy L. Beatty, Makedonka Mitreva, Peter U. Fischer

Journal Articles: Epidemiology

Wolbachia are endosymbionts of numerous arthropod and some nematode species, are important for their development and if present can cause distinct phenotypes of their hosts. Prophage DNA has been frequently detected in Wolbachia, but particles of Wolbachia bacteriophages (phage WO) have been only occasionally isolated. Here, we report the characterization and isolation of a phage WO of the southern ground cricket, Allonemobius socius, and provided the first whole-genome sequence of phage WO from this arthropod family outside of Asia. We screened A. socius abdomen DNA extracts from a cricket population in eastern Missouri by quantitative PCR for Wolbachia surface protein …


Dengue And The Lectin Pathway Of The Complement System, Romchat Kraivong, Nuntaya Punyadee, M Kathryn Liszewski, John P Atkinson, Panisadee Avirutnan Jan 2021

Dengue And The Lectin Pathway Of The Complement System, Romchat Kraivong, Nuntaya Punyadee, M Kathryn Liszewski, John P Atkinson, Panisadee Avirutnan

Open Access Publications

Dengue is a mosquito-borne viral disease causing significant health and economic burdens globally. The dengue virus (DENV) comprises four serotypes (DENV1-4). Usually, the primary infection is asymptomatic or causes mild dengue fever (DF), while secondary infections with a different serotype increase the risk of severe dengue disease (dengue hemorrhagic fever, DHF). Complement system activation induces inflammation and tissue injury, contributing to disease pathogenesis. However, in asymptomatic or primary infections, protective immunity largely results from the complement system's lectin pathway (LP), which is activated through foreign glycan recognition. Differences in N-glycans displayed on the DENV envelope membrane influence the lectin pattern …


Habitual Flavonoid Intake And Ischemic Stroke Incidence In The Danish Diet, Cancer, And Health Cohort, Benjamin H. Parmenter, Frederik Dalgaard, Kevin Murray, Aedin Cassidy, Catherine P. Bondonno, Joshua R. Lewis, Kevin D. Croft, Cecilie Kyrø, Gunnar Gislason, Augustin Scalbert, Anne Tjønneland, Jonathan M. Hodgson, Nicola P. Bondonno Jan 2021

Habitual Flavonoid Intake And Ischemic Stroke Incidence In The Danish Diet, Cancer, And Health Cohort, Benjamin H. Parmenter, Frederik Dalgaard, Kevin Murray, Aedin Cassidy, Catherine P. Bondonno, Joshua R. Lewis, Kevin D. Croft, Cecilie Kyrø, Gunnar Gislason, Augustin Scalbert, Anne Tjønneland, Jonathan M. Hodgson, Nicola P. Bondonno

Research outputs 2014 to 2021

Background

Flavonoid-rich foods have antiinflammatory, antiatherogenic, and antithrombotic properties that may contribute to a lower risk of ischemic stroke.

Objectives

We aimed to investigate the relationship between habitual flavonoid consumption and incidence of ischemic stroke in participants from the Danish Diet, Cancer and Health Study.

Design

In this prospective cohort study, 55,169 Danish residents without a prior ischemic stroke [median (IQR) age at enrolment of 56 y (52–60)], were followed for 21 y (20–22). We used Phenol-Explorer to estimate flavonoid intake from food frequency questionnaires obtained at study entry. Incident cases of ischemic stroke were identified from Danish nationwide registries …


Factors Driving The Compositional Diversity Of Apis Mellifera Bee Venom From A Corymbia Calophylla (Marri) Ecosystem, Southwestern Australia, Daniela Scaccabarozzi, Kenneth Dods, Thao T. Le, Joel P. A. Gummer, Michele Lussu, Lynne Milne, Tristan Campbell, Ben Pan Wafujian, Colin Priddis Jan 2021

Factors Driving The Compositional Diversity Of Apis Mellifera Bee Venom From A Corymbia Calophylla (Marri) Ecosystem, Southwestern Australia, Daniela Scaccabarozzi, Kenneth Dods, Thao T. Le, Joel P. A. Gummer, Michele Lussu, Lynne Milne, Tristan Campbell, Ben Pan Wafujian, Colin Priddis

Research outputs 2014 to 2021

Bee venom (BV) is the most valuable product harvested from honeybees ($30 - $300 USD per gram) but marginally produced in apiculture. Though widely studied and used in alternative medicine, recent efforts in BV research have focused on its therapeutic and cosmetic applications, for the treatment of degenerative and infectious diseases. The protein and peptide composition of BV is integral to its bioactivity, yet little research has investigated the ecological factors influencing the qualitative and quantitative variations in the BV composition. Bee venom from Apis mellifera ligustica (Apidae), collected over one flowering season of Corymbia calophylla (Myrtaceae; marri) was characterized …


Prevalence Of Anosmia Among Covid-19 Patients In Taif City, Kingdom Of Saudi Arabia, Adnan A. Mubaraki, Ghaida T. Alrbaiai, Afnan K. Sibyani, Rahaf M. Alhulayfi, Reema S. Alzaidi, Haneen S. Almalki Jan 2021

Prevalence Of Anosmia Among Covid-19 Patients In Taif City, Kingdom Of Saudi Arabia, Adnan A. Mubaraki, Ghaida T. Alrbaiai, Afnan K. Sibyani, Rahaf M. Alhulayfi, Reema S. Alzaidi, Haneen S. Almalki

Saudi Medical Journal

Objectives: To assess the prevalence of anosmia in coronavirus-19 (COVID-19) patients. Coronavirus-19-related anosmia and hyposmia is a new emerging concept in the medical literature. Methods: A retrospective study on COVID-19 patients with anosmia and hyposmia was performed during the period between May-July, 2020. The inclusion criteria were all clinically stable COVID-19 patients >15 years old with positive reverse transcription-polymerase chain reaction (RT-PCR). Patients who refused to participate or leave incomplete questions were excluded. In this study, we investigated 1022 patients who met our criteria. Results: Olfactory dysfunction (OD) was reported by 53% of our COVID-19 patients, of which 32.7% were …


Coronavirus Disease 2019 (Covid-19) In Pediatric Emergency. Presentation And Disposition, Roaa S. Jamjoom Jan 2021

Coronavirus Disease 2019 (Covid-19) In Pediatric Emergency. Presentation And Disposition, Roaa S. Jamjoom

Saudi Medical Journal

Objectives: To examine the demographics and common presentations of pediatric coronavirus disease 2019 patients in the emergency department (ED), as well as their contact with positive COVID-19 cases, return visits, and patients’ disposition from the ED. Methods: A retrospective chart review of confirmed cases of COVID-19 presenting to the Pediatric ED from March 2020 until June 2020 was conducted. Results: Fifty-two patients were identified, with a higher frequency of male patients. Forty-four (85%) patients were discharged from the ED, and 8 (15%) required admission. Three patients were admitted to the pediatric intensive care unit and 2 died, resulting in a …


Advantages And Disadvantages Of Telemedicine During The Covid-19 Pandemic Era Among Physicians In Taif, Saudi Arabia, Adnan A. Mubaraki, Aeshah D. Alrabie, Afnan K. Sibyani, Raghad S. Aljuaid, Ameerah S. Bajaber, Murad A. Mubaraki Jan 2021

Advantages And Disadvantages Of Telemedicine During The Covid-19 Pandemic Era Among Physicians In Taif, Saudi Arabia, Adnan A. Mubaraki, Aeshah D. Alrabie, Afnan K. Sibyani, Raghad S. Aljuaid, Ameerah S. Bajaber, Murad A. Mubaraki

Saudi Medical Journal

Objectives: To evaluate the advantages and disadvantages of telemedicine among physicians during the COVID-19 pandemic. To assess the awareness about telemedicine among physicians and determine their opinions about telemedicine in the post-pandemic era. Methods: A cross-sectional study was conducted at government hospitals (King Faisal Medical Complex [KFMC]-King Abdulaziz Specialist Hospital [KASH]) in Taif, Kingdom of Saudi Arabia (KSA), from May-August 2020. Taif is a small city in the western region of KSA with a population of 689,000 and 2 main hospitals (KFMC & KASH) which also serve rural areas close to Taif city. A total of 36 physicians practiced telemedicine, …


Get Privacy Trending: Best Practices For The Social Media Educator, Sara W. Dong, Nathanial S. Nolan, Miguel A. Chavez, Yijia Li, Gerome V. Escota, Wendy Stead Jan 2021

Get Privacy Trending: Best Practices For The Social Media Educator, Sara W. Dong, Nathanial S. Nolan, Miguel A. Chavez, Yijia Li, Gerome V. Escota, Wendy Stead

Open Access Publications

Social media is an increasingly popular forum for medical education. Many educators, including those in infectious diseases, are now creating and sharing unique and educational patient cases online. Unfortunately, some educators unknowingly threaten patient privacy and open themselves to legal liability. Further, the use of published figures or tables creates risk of copyright infringement. As more and more infectious diseases physicians engage in social media, it is imperative to create best practices to protect both patients and physicians. This summary will define the legal requirements of patient de-identification as well as other practical recommendations as they relate to use of …