Digital Commons Network^™

Characterization And Purification Of Muc13 Monoclonal Antibodies: Techniques And Applications, Shabnam Malik, Daniel Zubieta, Mohammed Sikander, Sheema Khan, Murali Yallapu, Meena Jaggi, Subhash Chauhan

Research Colloquium

Background: MUC13 is a transmembrane glycoprotein that is critically involved in the progression of various cancers, including liver, pancreatic, colon, and ovarian cancers. Despite the recognition of its presence and significance in these malignancies, the development of specific antibodies targeting MUC13 remains limited. This study aims to produce a monoclonal antibody against MUC13 and evaluate its effectiveness in assessing differential expression related to cancer progression.

Methodology: We utilized hybridoma technology to generate specific monoclonal antibodies targeting MUC13. Mouse splenocytes were immunized with recombinant MUC13 protein and subsequently fused with myeloma cells to create hybridomas. Positive clones were selected through ELISA …

Investigating Cucurbitacin D'S Efficacy In Ameliorating Benzo[A]Pyrene-Induced Hepatic Injury, Mohammed Sikander, Shabnam Malik, Daniel Zubieta, Anyssa Rodriguez, Fathi T. Halaweish, Murali Yallapu, Meena Jaggi, Subhash Chauhan

Research Colloquium

Hepatocellular carcinoma (HCC) is increasingly linked to various co-morbidity factors, notably smoking, which introduces benzo[a]pyrene—a potent carcinogen known for its role in liver damage. Cucurbitacin, a triterpene with diverse biological activities, exhibits antioxidant, antiinflammatory, and anti-cancer properties, yet its hepatoprotective effects remain inadequately explored. In this study, we investigated the hepatoprotective activity of cucurbitacin D, a novel analog, against benzo[a]pyrene-induced liver injury in human HepG2 cells. To evaluate its protective effects, we conducted proliferation, clonogenicity, migration, invasion assays, alongside Western blotting and qPCR analyses. The DCFDA assay was employed to measure intracellular reactive oxygen species (ROS) levels in liver cells. …

Disruption Of Β-Catenin Signaling As A Strategy To Suppress Emt In Cervical Cancer, Mohammed Sikander, Shabnam Malik, Sonam Kumari, John Apraku, Theresa M. Biju, Fathi T. Halaweish, Murali Yallapu, Meena Jaggi, Subhash Chauhan

Research Colloquium

Cervical cancer (CxC) is a leading cause of mortality and morbidity among women worldwide. Current chemotherapeutic agents for CxC have shown systemic toxicity in CxC patients. Ormeloxifene (ORM) is a non-toxic and non-steroidal drug with well-defined pharmacokinetic and pharmacodynamic properties in humans. In this study, we introduce a new analogue of ormeloxifene, Bromo-ormeloxifene (Br-ORM), which demonstrates enhanced therapeutic efficacy against CxC in both in vitro and in vivo models. We investigated Br-ORM's effects on CxC cell growth through colony formation and MTS assays, alongside molecular docking studies with βcatenin using AutoDock4. Furthermore, we analyzed the influence of Br-ORM on the …

An Unusual Case Of Spontaneous Bacterial Peritonitis Due To Weissella Cibaria In A Patient With Decompensated Cirrhosis, Janetly Reinoso, Felipe Diaz Jr, Jose E. Campo Maldonado

Research Colloquium

Background: Spontaneous bacterial peritonitis (SBP) is a serious infection commonly encountered in patients with decompensated cirrhosis and ascites, usually involving gram-negative organisms. Weissella cibaria is a gram-positive, catalase-negative, facultative anaerobe not typically associated with intra-abdominal infections. This case report highlights an unusual instance of SBP caused by W. cibaria in a cirrhotic patient, emphasizing the clinical importance of identifying atypical organisms and discussing implications for antimicrobial stewardship in vulnerable populations.

Case Presentation: A 65-year-old woman with decompensated nonalcoholic steatohepatitis (NASH) cirrhosis status post-transjugular intrahepatic portosystemic shunt (TIPS) placement and recurrent ascites requiring weekly paracentesis presented with altered mental status following …

From Imaging To Impact: A Multi-Disciplinary T32 Program Framework And Initial Insights For Training In Image-Guided Cancer Therapy, Kari J. Brewer Savannah, Stephen Y. Lai, Clifton D. Fuller, Kristy K. Brock

Advances in Cancer Education and Quality Improvement

As multi-disciplinary image-guided assessment and therapies become increasingly important for precision oncology, the need for comprehensive training opportunities that effectively prepare the next generation of clinician-scientists and scientists is evident. We present an innovative, evidence-based research training framework that integrates the following critical features: 1) intentional and personalized mentoring and career development, 2) immersive research in image-guided cancer therapy, 3) strategic mentored clinical exposure, 4) structured didactic learning and professional development opportunities, and 5) mentored grant writing. Designed to foster scientific rigor, translational thinking, and multi-disciplinary collaboration, the Image-Guided Cancer Therapy T32 Training Program (IGCT-T32) aims to prepare postdoctoral fellows …

Antiplatelet Effects Of Angelica Dahurica Root Extract Without Gastric Lesion Formation In Rats, Pitiphat Srisom, Kasem Rattanapinyopituk, Uthai Sotanaphun, Rataya Luechapudiporn

The Thai Journal of Pharmaceutical Sciences

Background: Platelet aggregation plays a crucial role in hemostasis; however, excessive platelet activation can lead to thrombus formation, contributing to cardiovascular diseases. While aspirin (acetylsalicylic acid [ASA]) is commonly used as an antiplatelet agent, its longterm use is associated with gastrointestinal side effects. Angelica dahurica (AD), a traditional herbal remedy, has shown in vitro antiplatelet activity and cyclooxygenase enzyme inhibition. However, its in vivo effects on platelet aggregation and gastric safety remain unclear.

Objectives: This study aimed to investigate whether Angelica dahurica root extract (ADE) could inhibit platelet aggregation and prevent aspirin-induced gastric lesions.

Materials and Methods: AD was extracted …

Bone-Sparing Effects Of Pueraria Mirifica Extract On Bone Loss In Androgen-Deficient Cynomolgus Macaques (Macaca Fascicularis), Thaweechai Saetae, Taratorn Kemthong, Sukanya Jaroenporn, Suchinda Malaivijitnond

The Thai Journal of Pharmaceutical Sciences

Background: Synthetic estrogens provide remarkable benefits for bone health in men; however, they also present several drawbacks after long-term use. Pueraria mirifica, a phytoestrogen-rich herb, has garnered attention due to its positive effects and minimal adverse side effects on bone in females. Most research on bone health has been conducted on rodents; however, bone architecture and remodeling in rodents differ from those in humans, making it challenging to apply the results to clinical trials.
Objectives: Thus, this study assessed the bonesparing effects of P. mirifica extract (PME) in androgen-deficient male cynomolgus macaques.

Materials and Methods: Adult male macaques were divided …

Quality By Design Approach To Method Development And Validation For Quantitative Estimation Of Dapagliflozin And Linagliptin In Pharmaceutical Tablet Dosage Forms, Prechha Shrestha, Ashwinee Kumar Shrestha, Rajan Shrestha, Barsha Thapa

The Thai Journal of Pharmaceutical Sciences

Objectives: The present study utilizes a quality by design approach to develop a simple, robust, and stability-indicating high-performance liquid chromatography method for quantifying dapagliflozin (DAPA) and linagliptin (LINA) in a fixed-dose combination and validate it according to the ICH-Q2 (R1) guidelines.

Materials and Methods: The effect of critical method parameters (CMPs), namely percentage of acetonitrile (ACN), pH of the buffer, and flow rate, on critical quality attributes (CQAs) such as retention time, area, tailing factor, and theoretical plates was studied using Box–Behnken design (BBD). Response optimizer was utilized to optimize the experimental conditions. The mobile phase (MP) consisted of ACN …

Cytotoxic And Anti-Inflammatory Activities Of Extracts Of Pra-Sa-Ka-Phrao Remedy And Its Plant Components, Theeraphong Ninlaor, Arunporn Itharat, Srisopa Ruangnoo, Pakakrong Thongdeeying, Sunita Makchuchit, Pranporn Kuropakornpong, Settakul Boonyasakseree, Chadchom Choockong, Ninnart Intharit, Neal M. Davies

The Thai Journal of Pharmaceutical Sciences

Background: Pra-Sa-Ka-Phrao (PSKP) is a traditional Thai remedy recognized in the National List of Essential Herbal Medicines of Thailand. It is traditionally used for treating flatulence and colic pain. It consists of eight components derived from plants with Ocimum tenuiflorum leaves as a main ingredient. However, there are no reports regarding the biological activities and chemical composition of PSKP.

Objectives: This study aimed to investigate cytotoxic activities, using cell lines related to the gastrointestinal (GI) system, and anti-inflammatory activities, along with chemical composition, of the PSKP complete formula (PSKPC) and a modified incomplete formula (PSKPIC).

Materials and Methods: Two extraction …

Continuous Glucose Monitoring Versus Self-Monitoring For Patients With Type 1 Diabetes: A Systematic Review And Meta-Analysis, Prayuth Poowaruttanawiwit, Kwanchai Rattanamanee, Nonnachanok Chaiyot, Saranpreeya Srisinroongruang

The Thai Journal of Pharmaceutical Sciences

Background: Patients with type 1 diabetes mellitus (T1DM) require intensive glucose monitoring to achieve optimal glycemic control and prevent complications. While self-monitoring of blood glucose (SMBG) has long been the standard approach, continuous glucose monitoring (CGM) offers real-time data and trend analysis that may enhance clinical decision-making.

Objective: This study aimed to evaluate whether CGM provides superior glycemic outcomes compared with SMBG in individuals with T1DM.

Materials and Methods: A systematic review and meta-analysis were conducted following preferred reporting items for systematic reviews and meta-analyses guidelines. Four randomized controlled trials involving T1DM patients using either CGM or SMBG were included. …

Comparative Study For Channel Pairing In Noma Ofdm- Cdma System, Hazem H. El-Banna, Nelly M. Shafik

Mansoura Engineering Journal

Orthogonal Multiple Access such as OFDM technique became inadequate scheme since it provides limited spectral efficiency and limited number of users. In addition to Orthogonal Multiple Access (OMA), Non-Orthogonal Multiple Access (NOMA) technique is applied in 5th generation of mobile communications that enhances both spectral efficiency and capacity with high Quality of Service (QoS). In this paper, three techniques were combined NOMA, OFDM, and CDMA in order to have optimal system performance in the presence of worst fading channel situations; fast frequency selective Rayleigh multi-path fading channel. The main contribution of this paper is the novel channel pairing technique introduced …

An Estimation Study Of The Co2 Emissions In Saudi Arabia Using Machine Learning, Mohammed A. Albalawi, M.M. El-Halwany, Mahmoud H. Mahmoud, Mohamed Gar Alalm

Mansoura Engineering Journal

As part of the 2015 Paris Climate Agreement, the Kingdom of Saudi Arabia (KSA) pledged to implement policies aimed at reducing greenhouse gas emissions. The 2030 Vision, which KSA unveiled with the goal of building a more sustainable and diverse economy, led to a number of projects, such as the national renewable energy program, the Saudi green initiative, and the circular carbon economy. Additionally, KSA just revealed a bold plan to achieve net-zero status by 2060. The Kingdom pledged to cut its carbon emissions by 278 million tons of CO2eq (equivalent) yearly by 2030 in its revised nationally determined contribution …

Isocratic Reversed-Phase High-Performance Liquid Chromatography Approach To Quantify Flutamide In Pharmaceuticals, Siddaraju Chikkalingaiah, Prathima Chikkegowda, Rajendraprasad Nagaraju

The Thai Journal of Pharmaceutical Sciences

Background: A non-steroidal antiandrogen, flutamide (FA), has been used often to treat advanced prostate cancer. It blocks testosterone’s tendency to bind to androgen receptors inside cells in some of the target organs, such as hair follicles, the prostate gland, skin, and testicles.

Objective: The main focus of the work is to produce an economical and reliable reversed-phase high-performance liquid chromatography technique for the quantitative study of FA in bulk materials and pharmaceuticals.

Materials and Methods: The elution of FA was done by running a mobile phase prepared by mixing 10 mM ammonium formate-formic acid buffer (pH-4) with methanol in the …

Screening And Evaluation Of Thai Herbal Plants As Multifunctional Agents For Targeting Amyloid Β Pathogenesis In Alzheimer's Disease, Ruedeemars Yubolphan, Suchinda Malaivijitnond, Sukanya Jaroenporn

The Thai Journal of Pharmaceutical Sciences

Background: Alzheimer's disease (AD) is a neurodegenerative disorder driven by complex pathophysiological processes. Current therapeutic strategies emphasize the development of multifunctional agents, particularly those derived from natural sources, to target multiple stages of the amyloid β (Aβ) cascade, a central contributor to AD pathology.

Objectives: This study aimed to investigate the anti-Aβ activity of nine Thai herbs and evaluate their potential as multifunctional agents for AD treatment.

Methods: Nine Thai herbs—Caesalpinia sappan (CS), Thunbergia laurifolia (TL), Rhinacanthus nasutus (RN), Tabernaemontana divaricata (TD), Cyperus rotundus (CR), Terminalia chebula (TC), Azadirachta indica (AI), Piper retrofractum (PR), and Asparagus racemosus (AR)—were screened …

From P-Values To Practical Relevance: An Introduction To Effect Sizes Through A Legal Psychological Example, Paul Riesthuis, Henry Otgaar, Tery Setiawan, Nathanael Sumampouw, Charlotte Bücken

Psychological Research on Urban Society

Null-hypothesis significance testing and p-values are frequently criticized for their focus on detecting non-zero differences and their inability to provide evidence for the null hypothesis. In this article, we highlight how effect sizes, when meaningfully interpreted, can address these issues. Specifically, we argue that researchers should consider the smallest effect size of interest (SESOI) - the smallest effect size that yields practical or theoretical relevance. We propose several methods for estimating the SESOI and present a consensus study among Indonesian professionals which can be used to estimate the SESOI for child eyewitness testimony research. Results suggest that most Indonesian professionals …

Novel Convolution Neural Network For Enhanced Network Attack Pattern Recognition, Salahaldeen Duraibi

University of Bisha Journal for Basic and Applied Sciences

Intrusion detection plays a critical role in safeguarding computer networks from malicious activities and unauthorized access. Traditional machine learning (ML) models have been widely employed for intrusion detection, but their effectiveness can be limited due to the complex and evolving nature of network attacks. An efficient Convolutional Neural Network (CNN) that utilizes one-dimensional convolutions, batch normalization, and max pooling layers is designed to capture the attack patterns in network traffic data. By leveraging the inherent feature extraction capabilities of CNNs, the proposed architecture demonstrates improved performance in identifying network intrusions compared to traditional ML models. The effectiveness of the novel …

Adaptive Ensemble Approaches For Face Mask Detection Using Transfer Learning Model, Faisal Alshanketi

University of Bisha Journal for Basic and Applied Sciences

Amidst the Covid-19 pandemic, wearing a mask has become mandatory and remains crucial in the ongoing pandemic. In certain situations and conditions, masks may be required periodically. However, manually monitoring large crowds of people to ensure they are wearing masks in public places or events can be an arduous task that is both time-consuming and difficult to manage. One way to address the challenge of manually monitoring mask-wearing in public places is to adopt machine learning (ML) methods and ICT-based surveillance mechanisms, thereby reducing the need for large numbers of personnel. While previous studies have proposed various machine learning models …

Radio Frequency (Rf) Based Smart Communication System For Autonomous Vehicles (Av) Covering Both Vehicle-To-Vehicle (V2v) And Vehicle-To-Traffic Light (V2tl), Rayan Hamza Alsisi, H. A. Wajid, M. O. Alrafaee, O. A. Alhaboob, A. Yousef

University of Bisha Journal for Basic and Applied Sciences

A vehicle that reaches a destination by self-navigation famously known as an autonomous or self-driving vehicle. In this work, we present an improved or smart communication system for autonomous vehicles that will enable them to reach distention by communicating a) with other vehicles (known as vehicle-to-vehicle V2V) and b) with the infrastructure of transportation system specifically traffic lights (known as vehicle to traffic lights V2TL). The proposed communication system is designed using several sensors, transmitters, and receivers to monitor the surroundings for efficient communication either V2V or V2TL. Finally, we show with experimented data that the proposed communication system offers …

Rutabaga Cultivation In The Lake Superior Region, Russell Magnaghi

Upper Country: A Journal of the Lake Superior Region

The vast expanse of the Lake Superior Basin has been home to a multitude of industries from copper and iron mining, timber extraction, steel mills, grain transfer ports, and ship construction. However, given its northern location, agriculture is usually ignored due to a short growing season and poor soils. Immigrants who settled there like Finns and Scandinavians were familiar with this environment but even Italians found that they could develop their cherished home gardens and small farms. Most immigrants had migrated from farming environments and now accepted the challenge to farm in this new environment.

Of the many crops that …

Intelligent Pharmacy: Leveraging Ai And Automation To Enhance Patient Care And Pharmacist Roles, Craig Kimble, Alberto Coustasse, Kasi Gorli, Thomas L. Pile

Management Faculty Research

AI and automation in pharmacies enhance clinical care, operational efficiency, and workforce optimization, with widespread adoption in hospitals and retail chains. Key benefits include reduced medication errors, improved patient satisfaction, and increased pharmacist productivity, allowing focus on higher-value clinical roles. Challenges include high initial costs, cybersecurity concerns, and resistance to change, necessitating thoughtful planning and stakeholder engagement. Preparing pharmacy students for technology-driven environments is essential for successful AI integration and ethical oversight in pharmacy practice.

Doc 2025-11 (Amended) The Faculty Hearing Committee On Grievances Bylaws And Operating Procedures, University Of Dayton. Executive Committee Of The Academic Senate

Senate Documents

No abstract provided.

Validation Of The Jefferson Health-Related Social Needs Screener, Sara Beachy, Kristin L. Rising, Richard W. Hass, Terry Hyslop, Isabella Muti, Mackenzie Kemp, Rhea E. Powell, Cara Martino, Baligh R. Yehia, Joseph G. Cacchione, Patricia C. Henwood

Jefferson Hospital Staff Papers and Presentations

INTRODUCTION: Screening for health-related social needs (HRSN) is a growing national health priority. While multiple HRSN screening tools currently exist, none to our knowledge have been evaluated using robust statistical analyses. The goal of this work is to provide results from a validation study of the Jefferson HRSN screener conducted across inpatient and outpatient settings.

METHODS: This retrospective cross-sectional psychometric study included HRSN assessments conducted across inpatient and outpatient settings with adult patients from March 2023 to May 2024. The study was conducted across a 17-hospital academic health system serving a diverse community in a 9-county area crossing two states …

Exploring Organizational Success: Strategies That Optimize Employee Performance In Small Businesses, Latoya Anthony

Walden Dissertations and Doctoral Studies

Employee retention challenges in small businesses can undermine organizational effectiveness and profitability. Small business leaders in the Midwestern United States service industry are particularly concerned with ineffective human resource (HR) strategies, as these shortcomings often contribute to reduced workforce engagement and higher turnover. Grounded in the resource-based view (RBV) theory, the purpose of this qualitative pragmatic inquiry study was to explore successful HR strategies that small business leaders in the Midwestern United States service industry use to sustain organizational effectiveness and overall profitability. The participants were six HR leaders from small service firms who had successfully implemented HR practices that …

Music For Self-Care, A Nurse Well-Being Strategy, Kristen Gilmore Msn, Rn, Cen, Tcrn, Ne-Bc, Nc-Bc, Allison Mccrea Mmt, Mt-Bc, Stephen Didonato Phd, Lcp

Jefferson Hospital Staff Papers and Presentations

Background

• Evidence suggests that individuals with trauma exposure, such as the impact of crisis such as COVID-19, may benefit from music therapy.
• In 2021, the Hospital and Healthsystem Association of Pennsylvania (HAP) recognized the importance of ensuring well-being of the nursing workforce identifying the vital role that nursing plays upholding the highest standards of patient care.
• HAP, in partnership with the Pennsylvania Council on the Arts, created a three year, first of its kind program, to support the provision of music therapy program services for healthcare workers.
• Through this grant-funded program, Jefferson Health- Pennsylvania hospitals, were awarded $162,000. This grant …

Combined Annual Modulation Dark Matter Search With Cosine-100 And Anais-112, N Carlin, J Y Cho, J J Choi, S Choi, A C Ezeribe, L E França, C Ha, I S Hahn, S J Hollick, S B Hong, E J Jeon, H W Joo, W G Kang, M Kauer, B H Kim, H J Kim, J Kim, K W Kim, S H Kim, S K Kim, W K Kim, Y D Kim, Y H Kim, Y J Ko, D H Lee, E K Lee, H Lee, H S Lee, H Y Lee, I S Lee, J Lee, J Y Lee, M H Lee, S H Lee, S M Lee, Y J Lee, D S Leonard, N T Luan, V H A Machado, B B Manzato, R H Maruyama, R J Neal, S L Olsen, H K Park, H S Park, J C Park, J S Park, K S Park, K Park, S D Park, R L C Pitta, H Prihtiadi, S J Ra, C Rott, K A Shin, D F F S Cavalcante, M K Son, N J C Spooner, L T Truc, L Yang, G H Yu, J Amaré, J Apilluelo, S Cebrián, D Cintas, I Coarasa, E García, M Martínez, Y Ortigoza, A Ortiz De Solórzano, T Pardo, J Puimedón, M L Sarsa, C Seoane

Faculty and Staff Publications

The annual modulation signal, claimed to be consistent with dark matter as observed by DAMA/LIBRA in a sodium-iodide based detector, has persisted for over two decades. COSINE-100 and ANAIS-112 were designed to test the claim directly using the same target material. COSINE-100, located at Yangyang Underground Laboratory in South Korea, and ANAIS-112, located at Canfranc Underground Laboratory in Spain, have been taking data since 2016 and 2017, respectively. Each experiment published its respective results independently. In this Letter, we present the results of an annual modulation search as a test of the signal observed by DAMA/LIBRA with the first three …

Comparison Of Pcr-Based Methods For The Detection Of Canned Tuna Species, Chloe P. Castanon, Denise Hernandez, Akshay N. Khetrapal, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Canned tuna is susceptible to mislabeling due to its high consumer demand, complex global supply chains, and range of prices. DNA barcoding of a short fragment of the mitochondrial control region (CR), termed the CR mini-barcode, has been established as an effective method for tuna species identification. However, the high level of DNA degradation in canned tuna products reduces the effectiveness of this method. Therefore, this study aimed to compare CR mini-barcoding to targeted (i.e., real-time or multiplex) PCR-based methods to determine the most effective approach for canned tuna species identification. DNA was extracted in duplicate from 24 canned tuna …

State Of California Cannabis Reporting, University Of Mississippi – National Center For Cannabis Research And Education

California

No abstract provided.

Ethnic Disparities In Thoracic Aortic Dissection And Rupture: A Retrospective Analysis Of Hispanic And Non-Hispanic White Populations, Manuel Gutierrez

Yale School of Medicine Physician Associate Program Theses

Background: Aortic dissections and ruptures are even more strongly life-threatening events for patients who are unable to access care promptly. A dissection can reach 50% mortality rate within 24 hours, and a rupture may exceed 90%. Risk factors such as hypertension, genetics, and age play a role in the development of these events; however, few studies have explored whether marginalized groups, such as Hispanics, experience aortic events more frequently. The primary objective of this investigation is to determine whether there is a difference in the frequency of thoracic aortic dissections or ruptures driven by ethnicity in patients from the Yale …

Identifying Factors Linked To A Higher Prevalence Of Posttraumatic Stress Disorder Among Younger Us Military Veterans, Jordan Hathorn

Yale School of Medicine Physician Associate Program Theses

Objectives: Posttraumatic stress disorder is a significant public health concern in the U.S., with military veterans disproportionately affected. Although younger veterans exhibit a higher prevalence of PTSD compared to their older counterparts, the mechanisms driving this age-related difference remain unclear. This study examined sociodemographic, trauma-specific, and psychosocial factors that may contribute to the elevated prevalence of PTSD in younger (age < 50) vs. older (age 50 and older) veterans.

Methods: Data were analyzed from the National Health and Resilience in Veterans Study (NHRVS), which surveyed a nationally representative sample of US military veterans (n=4,069).

Results: Younger veterans were three times more likely to screen positive for PTSD compared to …

Ud In The News Sept. 13-19, University Of Dayton

News Releases

Conversation and National Desk stories featuring UD expertise were picked up widely nationwide. In a piece for Association to Advance Collegiate Schools of Business, Vince Lewis wrote about giving students opportunities to launch startups.