Digital Commons Network^™

Orthogonal And Multiplexable Genetic Perturbations With An Engineered Prime Editor And A Diverse Rna Array, Qichen Yuan, Hongzhi Zeng, Tyler C Daniel, Qingzhuo Liu, Yongjie Yang, Emmanuel C Osikpa, Qiaochu Yang, Advaith Peddi, Liliana M Abramson, Boyang Zhang, Yong Xu, Xue Gao

Faculty, Staff and Students Publications

Programmable and modular systems capable of orthogonal genomic and transcriptomic perturbations are crucial for biological research and treating human genetic diseases. Here, we present the minimal versatile genetic perturbation technology (mvGPT), a flexible toolkit designed for simultaneous and orthogonal gene editing, activation, and repression in human cells. The mvGPT combines an engineered compact prime editor (PE), a fusion activator MS2-p65-HSF1 (MPH), and a drive-and-process multiplex array that produces RNAs tailored to different types of genetic perturbation. mvGPT can precisely edit human genome via PE coupled with a prime editing guide RNA and a nicking guide RNA, activate endogenous gene expression …

Age-Dependent Regulation Of Axoglial Interactions And Behavior By Oligodendrocyte Ankyring, Xiaoyun Ding, Yu Wu, Anna Vainshtein, Victoria Rodriguez, Emily Ricco, James T Okoh, Yanhong Liu, Daniel C Kraushaar, Elior Peles, Matthew N Rasband

Faculty, Staff and Students Publications

The bipolar disorder (BD) risk gene ANK3 encodes the scaffolding protein AnkyrinG (AnkG). In neurons, AnkG regulates polarity and ion channel clustering at axon initial segments and nodes of Ranvier. Disruption of neuronal AnkG causes BD-like phenotypes in mice. During development, AnkG is also expressed at comparable levels in oligodendrocytes and facilitates the efficient assembly of paranodal junctions. However, the physiological roles of glial AnkG in the mature nervous system, and its contributions to BD-like phenotypes, remain unexplored. Here, we show that oligodendroglia-specific AnkG conditional knockout results in destabilization of axoglial interactions in aged but not young adult mice. In …

Transition To Enteral Triazole Antifungal Therapy For Pediatric Invasive Candidiasis: Secondary Analysis Of A Multicenter Cohort Study Conducted By The Pediatric Fungal Network, Robert F T Bucayu, Craig L K Boge, Inci Yildirim, Martha Avilés-Robles, Surabhi B Vora, David M Berman, Tanvi S Sharma, Lillian Sung, Elio Castagnola, Debra L Palazzi, Lara Danziger-Isakov, Dwight E Yin, Emmanuel Roilides, Gabriela Maron, Alison C Tribble, Pere Soler-Palacin, Eduardo López-Medina, José Romero, Kiran Belani, Antonio C Arrieta, Fabianne Carlesse, Dawn Nolt, Natasha Halasa, Daniel Dulek, Sujatha Rajan, William J Muller, Monica I Ardura, Alice Pong, Blanca E Gonzalez, Christine M Salvatore, Anna R Huppler, Catherine Aftandilian, Mark J Abzug, Arunaloke Chakrabarti, Michael Green, Irja Lutsar, Elizabeth D Knackstedt, Sarah K Johnson, William J Steinbach, Brian T Fisher, Rachel L Wattier

Faculty, Staff and Students Publications

Of 319 children with invasive candidiasis, 67 (21%) transitioned from intravenous to enteral antifungal therapy. Eight (12%) transitioned back to intravenous antifungal therapy, one due to perceived treatment failure defined by clinical progression or worsening. Global treatment response at study completion was successful in 66 participants who transitioned to enteral therapy.

Hiding In Plain Sight: Eating Disorders In Diverse Populations - A Case For Comprehensive Medical Education, Tashalee R Brown, Madeline O Jansen, Drew Hirsch, Habiba Amir, Alexis E Duncan, Ginger E Nicol

2020-Current year OA Pubs

BACKGROUND: Training gaps regarding the diagnosis and management of eating disorders in diverse populations, including racial, ethnic, sexual, and gender minoritized groups, have not been thoroughly examined.

OBJECTIVE: This study aimed to examine resident physicians' knowledge and attitudes regarding eating disorders in diverse populations, with a focus on areas for improved training and intervention.

METHODS: Ninety-two resident physicians in internal medicine, emergency medicine, obstetrics/gynecology, psychiatry, and surgery at an academic center completed an online survey from 12/1/2020-3/1/2021, which comprised multiple choice and vignette-style open-ended questions to assess knowledge and attitudes toward the management and clinical presentations of eating disorders. Overall, …

Effectiveness Of An Online Food Shopping Intervention To Reduce Salt Purchases Among Individuals With Hypertension - Findings Of The Saltswitch Online Grocery Shopping (Ogs) Randomised Trial, Jason Hy Wu, Damian Maganja, Liping Huang, Kathy Trieu, Fraser Taylor, Eden M Barrett, Clare Arnott, Xiaoqi Feng, Aletta E Schutte, Gian Luca Di Tanna, Cliona Ni Mhurchu, Adrian J Cameron, Mark D Huffman, Bruce Neal

2020-Current year OA Pubs

BACKGROUND: Online grocery shopping is a growing source of food purchases in many countries. We investigated the effect of nudging consumers towards purchases of lower sodium products using a web browser extension.

METHODS: This trial was conducted among individuals with hypertension who shopped for their groceries online in Australia. From July 2021 to June 2023, participants were randomised to use the SaltSwitch Online Grocery Shopping web browser extension or continue their usual grocery shopping for 12 weeks. The SaltSwitch extension modified a retailer's online shopping interface to suggest similar but lower sodium alternative products to those initially selected. The primary …

Food For Thought: A Qualitative Assessment Of Medical Trainee And Faculty Perceptions Of Nutrition Education, Olivia Kunitsky, Mahdi Taye, Karla Feeley, Hugh Johnson, Abigail Glynn, Avery Stivale, Matthew Hamers, Alexis Notarianni, Sireesha Mamillapalli, Gabi Waite, Sonia Lobo

Department of Neurology Faculty Papers

BACKGROUND: The American Society of Clinical Nutrition recommends 37 to 44 h of undergraduate medical nutrition education. The Total Health Curriculum at Geisinger Commonwealth School of Medicine (GCSOM) contains 14 h of objective-based nutritional instruction. This study aimed to examine the perceptions of key stakeholders regarding the role of nutrition in medicine and to identify barriers, opportunities for improvement, and roles/responsibilities for innovative implementation of nutrition education.

METHODS: This exploratory, qualitative study employed a phenomenological approach and inductive coding process. Purposive sampling recruited medical students, undergraduate medical education (UME) faculty, physicians, and other healthcare professionals at GCSOM and affiliated clinical …

Refereed Research: A Sufficientarian Approach To Consumption Ethics In The Face Of Climate Change, Srinivas Venugopal, Ronika Chakrabarti

Subsistence Marketplaces

In this paper, we adopt a sufficientarian ethics approach to study the concern of consumption inadequacy engendered by climate change in subsistence communities. The sufficientarian approach to consumption ethics dictates that in the face of climate change, priority should be afforded to establishing and maintaining consumption adequacy in low-income settings. We elaborate on the basic principles underlying sufficientarian ethics and compare it to alternative approaches such as utilitarian ethics and prioritarian ethics. Subsequently, we turn our attention toward specifying how poverty and the deleterious impact of climate change combine to render subsistence communities more vulnerable to consumption inadequacies as compared …

“Strange And Unusual Things”: Teaching The Speculative Fiction Of Margaret Cavendish, E Mariah Spencer

ABO: Interactive Journal for Women in the Arts, 1640–1830

Margaret Cavendish, a prolific author and natural philosopher, defies categorization with her multifaceted contributions to literature and philosophy. While she is recognized for her groundbreaking work in various academic fields, her literature also resonates within the realms of science fiction, weird fiction, and speculative genres. This essay explores innovative approaches to teaching Cavendish's work, particularly within the realm of speculative literature, using an interdisciplinary lens. Drawing on perspectives including monster theory and the concept of the abject, students are prompted to critically engage with Cavendish's texts. By examining themes such as the monstrous, the supernatural, and the occult, students uncover …

Toxic Varnishes: Perfumes, Stench, And Glow In Eighteenth-Century French Salons, Érika Wicky

ABO: Interactive Journal for Women in the Arts, 1640–1830

As several historians, including Alain Corbin, have noted, a new sensitivity to odors emerged in the 18^th century that increased people’s negative responses to strong smells and heightened the demand for fragrances that were not only pleasant but also light and subtle. Some scholars see this period as a true “olfactory revolution.” How was this expressed in the salon, perceived both as a luxurious space and a site of elite sociability? This focus on the smell of varnish aims to illustrate how the scent of materials intertwined with the pleasures of decorative arts, both in the entire space of …

What Is It Like To Be A Cognitive Ethologist?, Anne Wordsworth She/Her

Animal Sentience

Donald Griffin not only made the study of animal minds acceptable for scientific investigation but the work of the cognitive ethologists that he inspired made animals and their lives fascinating and accessible to the greater public. The scrupulously detailed examination of his life and work by Ristau ensures that this history will always be there to respect his work and his legacy

Leading Towards The Queerest Insurrection: Queer Anarchism And Leadership Studies, Josie Holland

Interdisciplinary Journal of Leadership Studies

Leadership shapes our world, our narratives, and what is possible to imagine. Unfortunately, leadership studies has some trouble imagining queer leadership, or rather, queer theory and leadership studies. In order to integrate the more abstract aspects of queer theory with the largely evidence-based field of leadership studies, I draw on the texts, history, practices, and theory of queer anarchism. This article aims to highlight a gap in leadership studies as it currently stands, and through accounting for the absence of queer anarchism in leadership studies, expand what leadership looks like. To this end, I suggest the framing of ‘leadership acts’ …

Utilitarian And Self-Representational: Player-To-Player Character Relationships In Final Fantasy Xiv, Zu Er Guan

Theses

This thesis studies the relationships between video game players and their player characters, the characters that they control, through the lens of player purpose and intent by conducting research using an online survey developed and analyzed using an interdisciplinary methodology based on existing studies in the field regarding avatar identification, player psychology, and queer and feminist theory. This thesis posits that are two types of player relationships with their player characters based on player intent—player characters as the personal avatar and representation of the self, and player characters as a tool for the execution of player control and gameplay experience. …

From Surviving To Thriving: Key Considerations For Weight Control Across Diverse Cancer Survivorship Populations, Justin C. Brown, Marlyn Allicock, Carmina G. Valle, Tanya Agurs-Collins

School of Medicine Faculty Publications

BACKGROUND: Obesity is a chronic, relapsing, progressive disease of excess adiposity that increases the risk of dying from at least 16 types of cancer. The prevalence of obesity has increased more rapidly in cancer survivors compared with the general population. Tailored weight management strategies are needed to improve prognosis and health outcomes in the growing population of cancer survivors. However, certain cancer survivor population subgroups require unique consideration when developing weight management strategies. METHODS: In a symposium convened by The Obesity Society during ObesityWeek 2023 titled "From Surviving to Thriving: Key Considerations for Weight Control Across Diverse Cancer Survivorship Populations," …

Course Structure And Social Outcomes Of Teaching Older Adults To Play Piano In A Group Setting: A Case Study Of Chinese Elderly Participants, Pinlin Liu, Derek Polischuk, Bob Lee

Visions in Leisure and Business

As the aging population continues to grow, a significant concern for this demographic is their living arrangements. Many older adults prefer to remain in their own homes after retirement rather than transition to long-term care facilities. However, the desire for independent living, particularly among the very elderly, often leads to social disconnection and the risk of social isolation. Facilitating opportunities for social engagement is essential to maintaining the social well-being of older adults. Research underscores the role of leisure activities in fostering skill development and social interaction. In this study, group-based piano lessons were introduced as a platform for older …

How Ngos Operationalize The Ecological Capability Approach To Achieve Sustainability Goals, Patrick Elven P. Bañaco, Maria Assunta C. Cuyegkeng

Journal of Management for Global Sustainability

This study explores how NGOs achieve sustainability goals as they focus on poverty alleviation through ecological capability building. In-depth semi-structured interviews with nine representatives of NGOs working on poverty alleviation were conducted and subjected to thematic analysis within a critical realist perspective. Findings show that the NGOs engage their communities in all the different stages of their programs, creating a sense of empowerment that becomes the foundation for their ecological meta-capability, a holistic approach to the development of human capability that considers social, economic, and environmental aspects. This is better understood by analyzing the interplay of Structure, Agency, and Culture …

Experimental Insights Into Friction Losses Of Post-Tensioned Precast Full-Scale Girders, Mostafa Serry

Theses and Dissertations

The use of post-tensioning in construction has revolutionized the construction industry and made way for several construction and structural applications that were unattainable before. One of the most important topics that are synonymous with the use of post-tensioning is the matter of accurately calculating the short-term and long-term losses that arise in pre-stress. One of the prominent types of losses is the friction losses that occur during the jacking of the concrete elements due to its impact on structural integrity and longevity.

The thesis examines this critical aspect of friction losses in post-tensioned concrete structures. The study focuses on full-scale, …

Exploring Predictors Of Purchase Intention Toward Plant-Based Meat, Kristiana Ginelle D. Monares, Ken Sean D. Roque, Melissa A. Santos, Sofia Marianne S. So, Catherine A. Valentin, Alvin Patrick M. Valentin

Journal of Management for Global Sustainability

Plant-based meat (PBM) presents itself as a sustainable alternative to meat production systems that have adversely impacted consumers’ health and the environment at large. In the Philippine context, however, there are only a few PBM manufacturers to date even though production has been emerging with expanded options for consumers to consider. Moreover, while PBM’s potential allows it to be a prospective working food system in the country through the promotion of healthier food chains, the literature on PBM in the Philippines is scant. This study thus contributes to this literature by exploring predictors of purchase intention toward PBM. The researchers …

Comparison Of Three Chimeric Zika Vaccine Prototypes Developed On The Genetic Background Of The Clinically Proven Live-Attenuated Japanese Encephalitis Vaccine Sa14-14-2, Byung-Hak Song, Jordan C. Frank, Sang-Im Yun, Justin G. Julander, Jeffrey B. Mason, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Xin Dai, Young-Min Lee

Animal, Dairy, and Veterinary Science Student Research

Zika virus (ZIKV) is a medically important mosquito-borne orthoflavivirus, but no vaccines are currently available to prevent ZIKV-associated disease. In this study, we compared three recombinant chimeric viruses developed as candidate vaccine prototypes (rJEV/ZIKV^MR-766, rJEV/ZIKV^P6-740, and rJEV/ZIKV^PRVABC-59), in which the two neutralizing antibody-inducing prM and E genes from each of three genetically distinct ZIKV strains were used to replace the corresponding genes of the clinically proven live-attenuated Japanese encephalitis virus vaccine SA₁₄-14-2 (rJEV). In WHO-certified Vero cells (a cell line suitable for vaccine production), rJEV/ZIKV^P6-740 exhibited the slowest viral growth, formed …

Role Of Nf2 Mutation In The Development Of Eleven Different Cancers, Shervin Hosseingholi Nouri, Vijay Nitturi, Elizabeth Ledbetter, Collin W English, Sean Lau, Tiemo J Klisch, Akash J Patel

Faculty, Staff and Students Publications

Background/Objectives: With the rise in prevalence of diagnostic genetic techniques like RNA sequencing and whole exome sequencing (WES), as well as biological treatment regiments for cancer therapy, several genes have been implicated in carcinogenesis. This review aims to update our understanding of the Neurofibromatosis 2 (NF2) gene and its role in the pathogenesis of various cancers. Methods: A comprehensive search of five online databases yielded 43 studies that highlighted the effect of sporadic NF2 mutations on several cancers, including sporadic meningioma, ependymoma, schwannoma, mesothelioma, breast cancer, hepatocellular carcinoma, prostate cancer, glioblastoma, thyroid cancer, and melanoma. Of note were key biological …

Vaccines Targeting P53 Mutants Elicit Anti-Tumor Immunity, Dafei Chai, Xu Wang, Chunmei Fan, Junhao Wang, Jing Ming Lim, Xinfang Yu, Ken H Young, Yong Li

Faculty, Staff and Students Publications

The p53 tumor suppressor is commonly mutated in cancer; however, there are no effective treatments targeting p53 mutants. A DNA vaccine gWIZ-S237G targeting the p53 S237G mutant, which is highly expressed in A20 murine tumor cells, was developed and administered intramuscularly via electroporation, either alone or in combination with PD1 blockade. The anti-p53-S237G immunization elicited a robust protective response against subcutaneous A20 tumors and facilitated the infiltration of immune cells including CD8+ T cells, NK cells, and DCs. The vaccine enhanced the induction and maturation of CD11c+, CD103+CD11c+, and CD8+CD11c+ cells, which in turn promoted tumor-specific antibody production, as well …

Documentary On Floating Wetlands, Nazish Abro

MSJ Capstone Projects

I created this documentary on floating wetlands as part of a climate project led by the Student Society of Climate Change during my degree program. This project is a competition among South Asian countries to find solutions for climate change. My team, PK Team 22 from IBA Karachi, is among the top three teams in Pakistan and has reached the final round. The top two teams will receive a US fellowship and seed funding to continue their work.

We received $200 from the US Consulate to complete the second phase of our project. I chose this topic not only for …

Hauntings Of Publication Deaths, Possibilities For Our Academic Present, Kathleen (Kaye) A. Hare, Amber Moore

Journal of Feminist Scholarship

In this paper, we use a feminist dialogue to query publication deaths that haunt us. We ruminate on respective past publications that remain unread and uncited, despite encapsulating defining events in our development as scholars. For Author Kaye Hare this was an affective narrative that troubled a seemingly ideal moment of public scholarship; for Author Amber Moore, this was a hard-fought analysis of precarious intersectional feminist resistance in popular culture. Inspired by the horror-film genre “spectral incognizance,” we methodologically deploy a multivocal dialogical structure based on multiple revisitings that attenuate anxieties of metaphorical publication death, before revealing that this death …

Using Gis To Visualize Daily Routines At The Neighborhood And Household Scales: A Feminist Approach Using A Nineteenth-Century Diary, Sterling D. Quinn

Journal of Feminist Scholarship

Feminist scholars in the discipline of geography have noted the ways that routine activities at the household and neighborhood scales contribute to power relations and identity formation. Geographic information systems (GIS), with their ability to create multiple visual representations of people’s experiences in space at any scale, hold great potential for revealing these local processes and patterns that are often lost or invisible in broader historical narratives, especially when they pertain to women and children. This article contributes an example by using GIS to encode and map everyday events in a diary kept by Elisabeth Koren, a nineteenth-century Norwegian immigrant …

Folic Acid And Methyltetrahydrofolate Supplementation In The, Karen E Christensen, Marie-Lou Faquette, Daniel Leclerc, Vafa Keser, Yan Luan, Jeanna L Bennett-Firmin, Olga V Malysheva, Alaina M Reagan, Gareth R Howell, Marie A Caudill, Teodoro Bottiglieri, Rima Rozen

Faculty Research 2024

Background/Objectives: The MTHFR677C>T gene variant results in a thermolabile MTHFR enzyme associated with elevated plasma homocysteine in TT individuals. Health risks associated with the TT genotype may be modified by dietary and supplemental folate intake. Supplementation with methyltetrahydrofolate (methylTHF) may be preferable to folic acid because it is the MTHFR product, and does not require reduction by DHFR to enter one-carbon folate metabolism. In the Mthfr677C>T mouse model for this variant, female 677TT (TT) mice have an increased incidence of hepatic steatosis. The objective of this study was to compare the effects of methylTHF and folic acid supplementation …

Physical Therapy Student Spotlight: Megan Garro '25, University Of Dayton. School Of Education And Health Sciences

School of Education and Health Sciences Blog Archive

Garro is excelling in the classroom and in the field, and she's looking forward to helping people bounce back after an injury.

"They Don't Get It": Marginalized Mothers And Their Experiences With Social Support", Zuleida Herba

Counseling and Psychology Dissertations

Maternal caregivers of children with developmental disabilities (DD) experience a significant burden of care and increased parenting stress, making social support crucial to family well-being. This qualitative study used a feminist, intersectional framework to examine how marginalized mothers of children with DD, who have been less extensively studied than majority mothers (Stober & Franzese, 2018), perceive and experience social support from extended family, friends and community members. It also examined how social supports impact the mother’s quality of life, understanding that the caregiver's well-being is a determining factor for the child's well-being. The study investigated, from mothers’ perspectives, what kind …

A Quantitative Approach To Evaluating Multi-Event Resilience In Oil Pipeline Incidents, Labiba N. Asha, Nita Yodo, Ying Huang

Industrial Engineering Faculty Publications and Presentations

This study introduces a quantitative approach to evaluating the resilience of oil pipeline systems against various natural and physical disruptions. Resilience is increasingly essential in critical infrastructure to ensure continuous operations and minimize disruption impacts. However, existing quantitative methods often need specific time-dependent data, making measuring resilience in pipeline infrastructure challenging. To address this gap, this paper proposed a comprehensive framework by integrating the existing incident database with key features of assessing failure probabilities based on historical events and developing multi-event resilience indicators based on system performance under various disruptions. The methodology employs event tree analysis to quantify the probabilities …

Swimming Against The Tide: Resistance Strategies And Agentic Skills Used By Childfree Women, Carolina Rojas-Madrigal

Journal of Feminist Scholarship

In pronatalist societies, the decision to remain childfree creates a conflict-laden scenario. This article explores how childfree women confront social hostility and defend their reproductive autonomy through resistance strategies and tactics. With this aim, the results of a feminist research, positioned from feminist Standpoint theory and digital ethnography, are presented. The researcher conducted biographical-narrative interviews with sixteen voluntary childless women from Mexico, Guatemala, Costa Rica, Ecuador, Peru, Spain, and the United Kingdom, all of whom were members of a childfree Facebook group. This research revealed that there is a persistent tension between societal pressure placed on these women in pronatalist …

Agfi-Gan: An Attention-Guided And Feature-Integrated Watermarking Model Based On Generative Adversarial Network Framework For Secure And Auditable Medical Imaging Application, Xinyun Liu, Ronghua Xu, Chen Zhao

Michigan Tech Publications

With the rapid digitization of healthcare, the secure transmission of medical images has become a critical concern, especially given the increasing prevalence of cyber threats and data privacy breaches. Medical images are frequently transmitted via the Internet and cloud platforms, making them susceptible to unauthorized access, tampering, and theft. While traditional cryptographic techniques play a vital role, they are often insufficient to fully ensure the integrity and confidentiality of these sensitive images. In this paper, we present AGFI-GAN, a robust and secure framework for medical image watermarking that leverages attention-guided and Feature-Integrated mechanisms within a Generative Adversarial Network (GAN). Specifically, …

From Vacant Lots To Paved Roads: Exploring West Whittier- Los Nietos, Marianne Edith Vazquez-Ochoa

Whittier City & College Historical Research

West Whittier- Los Neitos history of Latino in neighborhood like Jimtown in Whittier California