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Defining The Discipline: Six Pillars Of Academic Success Programming In Law Schools, Kris Franklin, Catherine Martin Christopher

Articles & Chapters

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Methylome–Proteome Integration After Late-Life Voluntary Exercise Training Reveals Regulation And Target Information For Improved Skeletal Muscle Health, Toby L Chambers, Andrea Dimet-Wiley, Alexander R Keeble, Amin Haghani, Wen-Juo Lo, Gyumin Kang, Robert Brooke, Steve Horvath, Christopher S Fry, Stanley J Watowich, Yuan Wen, Kevin A Murach

Physiology Faculty Publications

Abstract: Exercise is a potent stimulus for combatting skeletal muscle ageing. To study the effects of exercise on muscle in a preclinical setting, we developed a combined endurance–resistance training stimulus for mice called progressive weighted wheel running (PoWeR). PoWeR improves molecular, biochemical, cellular and functional characteristics of skeletal muscle and promotes aspects of partial epigenetic reprogramming when performed late in life (22–24 months of age). In this investigation, we leveraged pan-mammalian DNA methylome arrays and tandem mass-spectrometry proteomics in skeletal muscle to provide detailed information on late-life PoWeR adaptations in female mice relative to age-matched sedentary controls (n = 7–10 …

Nonresolving Neuroinflammation Regulates Axon Regeneration In Chronic Spinal Cord Injury, Andrew N. Stewart, Christopher C. Bosse-Joseph, Reena Kumari, William M. Bailey, Kennedy A. Park, Victoria K. Slone, John C. Gensel

Physiology Faculty Publications

Chronic spinal cord injury (SCI) lesions retain increased densities of microglia and macrophages. In acute SCI, macrophages induce growth cone collapse and facilitate axon retraction away from lesion boundaries. Little is known about the role of sustained inflammation in chronic SCI or whether chronic inflammation affects regeneration. We used the colony-stimulating factor-1 receptor inhibitor, PLX-5622, to deplete microglia and macrophages months after complete crush SCI in female mice. Transcriptional analyses revealed a significant inflammatory depletion within chronic SCI lesions after PLX-5622 treatment. Both transcriptional analyses and immunohistochemistry revealed that Iba1⁺ cells repopulate to predepleted densities after treatment removal. Neuronal-enriched …

Programmable Scanning Diffuse Speckle Contrast Imaging Of Cerebral Blood Flow, Faezeh Akbari, Xuhui Liu, Fatemeh Hamedi, Mehrana Mohtasebi, Li Chen, Lei Chen, Guoqiang Yu

Physiology Faculty Publications

Significance: Cerebral blood flow (CBF) imaging is crucial for diagnosing cerebrovascular diseases. However, existing large neuroimaging techniques with high cost, low sampling rate, and poor mobility make them unsuitable for continuous and longitudinal CBF monitoring at the bedside. Aim: We aimed to develop a low-cost, portable, programmable scanning diffuse speckle contrast imaging (PS-DSCI) technology for fast, high-density, and depth-sensitive imaging of CBF in rodents. Approach: The PS-DSCI employed a programmable digital micromirror device (DMD) for remote line-shaped laser (785 nm) scanning on tissue surface and synchronized a 2D camera for capturing boundary diffuse laser speckle contrasts. New algorithms were developed …

Racial And Rural Disparities In Financial Toxicity And Healthcare Transitions Among Adolescent And Young Adult Cancer Survivors In Kentucky, Jean S. Edward, Brent J. Shelton, Lauren Corum, Haafsah Fariduddin, Katie Brown, John A. D’Orazio, Kimberly D. Northrip

Physiology Faculty Publications

Introduction: Adolescent and young adult cancer survivors, especially racial/ethnic minorities and rural residents are particularly vulnerable to financial toxicity due to limited healthcare access, socioeconomic disparities, and cultural/language barriers. These social determinants of health compound financial hardship and contribute to poor healthcare transitions from pediatric to adult care, leading to worse outcomes and higher mortality rates. Methods: Our cross-sectional survey study examined racial (Black vs White) and geographic (rural vs urban) disparities in financial toxicity and healthcare transition outcomes among 260 adolescent and young adult cancer survivors through the Kentucky Cancer Registry. Survey data were collected on financial toxicity, healthcare …

Differential Effects Of Myosin Activators On Myocardial Contractile Function In Nonfailing And Failing Human Hearts, Joohee Choi, Patrick T. Wood, Joshua B. Holmes, Katherine L. Dominic, Cristobal G. Dos Remedios, Kenneth S. Campbell, Julian E. Stelzer

Physiology Faculty Publications

The second-generation myosin activator danicamtiv (DN) has shown improved function compared with the first-generation myosin activator omecamtiv mecarbil (OM) in nonfailing myocardium by enhancing cardiac force generation but attenuating slowed relaxation. However, whether the functional improvement with DN compared with OM persists in remodeled failing myocardium remains unknown. Therefore, this study aimed to investigate the differential contractile responses to myosin activators in nonfailing and failing myocardium. Mechanical measurements were performed in detergent-skinned myocardium isolated from donor and failing human hearts. Steady-state force, stretch activation responses and loaded shortening velocity were analyzed at submaximal [Ca2+] in the absence or presence of …

Alternate Quantification Approaches For Cold-Induced Vasodilation In Human Glabrous Skin, J. A. Stout, D. E. Gerow, P. C. Clegg, K. Metzler-Wilson, T. E. Wilson

Physiology Faculty Publications

Introduction: Cold-induced vasodilation (CIVD) is a counterintuitive focal increase in glabrous skin blood flow during cold exposure with unclear local and neural mechanisms. Methods: We tested 12 (8 men, 4 women) healthy subjects’ laser-Doppler flux (LDF; just proximal to the nailbed) and arterial blood pressure (ABP) on a beat-by-beat basis. The experimental hand was exposed to warm (10 min 35°C) and then cold (30 min 8°C) water immersion and the contralateral control hand experienced 22°C–23°C air throughout. We analyzed beat-by-beat oscillations in LDF and ABP via a fast-Fourier transform (FFT) and transfer function analysis (TFA) of LDF to ABP. Results: …

Gastrocnemius Myofiber Type And Mitochondrial Alterations Associated With Peripheral Artery Disease Severity, Kate Kosmac, Rena Dana Wang, Jada Stewart, Parminder Kaur, Ahmed Ismaeel, Haseeb Ahsan, Lisa Hartnell, Esther E. Dupont-Versteegden, Mary M. Mcdermott, Robert L. Sufit, Luigi Ferrucci, Charlotte A. Peterson

Physiology Faculty Publications

The extent of walking impairment varies among individuals with peripheral artery disease (PAD), which may reflect differences in the adaptability of lower extremity muscles to ischemia-reperfusion injury characteristic of the disease. Analyses of gastrocnemius muscle biopsies from 113 individuals with PAD [mean ankle-brachial index (ABI) = 0.65 ± 0.13, 38 (33.6%) women, 76 (67.2%) Black] showed a wide range of myofiber type distributions (9.6%-82.6% type 1 myofibers). The abundance of oxidative type 1 myofibers negatively correlated with ABI (r = −0.22, P = 0.02), a measure of PAD severity. The abundance of type 1 myofibers also negatively correlated to 2a/x …

Inter-Rater Reliability Of Muscle Ultrasonography Performed By Multidisciplinary Novice Sonographers In The Evaluation Of Critically Ill Patients With Acute Kidney Injury Requiring Continuous Kidney Replacement Therapy, Felipe González-Seguel, Vinh Q. Tran, Chaitanya Anil Pal, Zan T. Shareef, Hayley P. Israel, Arimitsu Horikawa-Strakovsky, Yuan Wen, Benjamin R. Griffin, Javier A. Neyra, J. Pedro Teixeira, Kirby P. Mayer

Physiology Faculty Publications

Early diagnosis of muscle wasting in critically ill patients with acute kidney injury requiring continuous kidney replacement therapy (AKI-CKRT) may improve outcomes via timely rehabilitation and nutrition. Muscle ultrasound (MUS) has recently gained traction for assessing muscle atrophy in the intensive care unit (ICU) but requires training to achieve reproducibility. We evaluated the inter-rater reliability of MUS in patients with AKI-CKRT performed by multidisciplinary raters including nephrologists. Two blinded independent raters used portable ultrasound to acquire images of the rectus femoris (RF). All raters were clinicians routinely caring for patients with CKRT in the ICU and were initially novices in …

Sex-Based Differences In The Long-Term Fate Of Hippocampal Neurons Born After A Traumatic Brain Injury, Hannah C. Downing, Ashley B. Glover, Jessica E. Gebhardt, Katherine L. Thompson, Kathryn E. Saatman

Physiology Faculty Publications

Introduction: Moderate-to-severe traumatic brain injury (TBI) results in an early loss of immature hippocampal granule cells and the activation of typically quiescent neural stem cells (NSCs) in the dentate gyrus. Activation of NSCs leads to a robust increase in proliferation and generation of neural progenitor cells (NPCs), supporting restoration of the immature neuron population of over a period of 1–2 weeks. However, it is unclear if neurons born early after injury develop normally, survive long-term and functionally integrate into the hippocampal network. Although adult hippocampal neurogenesis is regulated in a sex-dependent manner, the majority of pre-clinical TBI studies lack the …

Skeletal Muscle Stem Cell Mitochondria Are Transferred To Muscle Fibers In Response To A Hypertrophic Stimulus, Jensen Goh, Julia G. Williams, Sarah E. Ogle, Jai K. Joshi, Logan N. Scott, Benjamin Burke, Alex R. Keeble, Nicholas T. Thomas, Christopher S. Fry, Ahmed Ismaeel, John J. Mccarthy

Physiology Faculty Publications

The fusion of skeletal muscle stem cell (MuSC) to myofibers during hypertrophy has exclusively focused on the transfer of the MuSC nucleus, leaving the fate of other MuSC organelles, such as mitochondria, largely unexplored. The objective of this study was to determine whether MuSCs transfer their mitochondria upon myofiber fusion in response to a hypertrophic stimulus. To achieve this goal, we specifically labeled MuSC mitochondria with Dendra2 fluorescence by crossing the MuSC-specific CreER (Pax7^CreER/CreER) mouse with the Rosa26-Dendra2 mouse to generate the Pax7-Dendra2 mouse. To induce the fusion of MuSC to myofibers, Pax7-Dendra2 mice underwent synergist ablation surgery …

Governing Intelligence: Singapore’S Evolving Ai Governance Framework, Jason G. Allen, Jane Loo, Jose Luna

Research Collection Yong Pung How School Of Law

This paper provides an outline analysis of the evolving governance framework for Artificial Intelligence (AI) in Singapore. Across the Singapore government, AI solutions are being adopted in line with Singapore’s “Smart Nation Initiative” to leverage technology to make impactful changes to the nation and the economy. In tandem, Singaporean authorities have been assiduous to release a growing number of governance documents, which we analyse together to chart the city-state’s approach to AI governance in international comparison. Characteristics of Singapore’s AI governance approach include an emphasis on consensusbuilding between stakeholders (particularly government and industry but also citizens) andvoluntary or “quasi” regulation, …

Association Of Social Support And Burnout Among Undergraduate Medical And Health Sciences Students At A Malaysian University, Lee Jia Le, Farrah Asyiqin Mansor, Kayalvili R. Manogar,, Siti Khadijah Adam

Health Professions Education

Purpose: Medical and health sciences students are prone to burnout due to intense academic pressure and demanding schedule. Social support plays a vital role in mitigating burnout by providing emotional, informational, and practical assistance, which helps students navigate the challenges of their rigorous academic environment. The aim of this study was to determine the prevalence of burnout and the contributing factors among these students in a university in Malaysia. The association between social support and burnout among these students was also explored.

Method: A cross-sectional study was conducted among 459 undergraduate students in the Faculty of Medicine and Health Sciences, …

Faculty Relationships: A Key Factor In Fostering Students' Sense Of Belonging In A Doctor Of Physical Therapy Program, Gina Gang, Jenni Rae Rubio, Michael Antich, Anayansi Balmaceda, Alexandra Candamil, Lacey Fogata, Corey Salce, Roger Vermont Zelaya, Noha Daher

Health Professions Education

Background: Sense of belonging is critical for student success in higher education. Limited research exists investigating factors perceived by Doctor of Physical Therapy (DPT) students contributing to belonging during on-campus curricula.

Objective: The purpose of this study was to explore belonging among second-year DPT students and identify influencing factors and strategies to enhance on-campus belonging.

Methods: The study used a convergent parallel design, integrating quantitative cross-sectional survey data with qualitative thematic analysis. Second-year DPT students in one residential program completed the University Belonging Questionnaire (UBQ) and answered open-ended questions. The primary outcomes were degree of student belonging, factors influencing belonging, …

Seahorsedad: A Study Of Metaphors In Transmasculine Birther’S Instagram Posts, Alexa Anne Runnion

Graduate Student Theses, Dissertations, & Professional Papers

Dominant notions of pregnancy frame gestation as a solely female process. However, the experiences of transmasculine birthers challenge this assumption, demonstrating that pregnancy is not inherently tied to womanhood but is instead shaped by social and cultural narratives and representations. This paper explores the unique pregnancy and parental exercises of transmasculine birthers by examining public social media posts under the hashtag #seahorsedad on Instagram. Utilizing metaphorical criticism, I aim to uncover how transmasculine birthers, known within the community as “seahorse dads,” both uphold and resist traditional notions of gender, pregnancy, and parenting through a combination of textual and visual metaphor. …

Spatial And Temporal Patterns Of Nutrient Limitation In A Western Montana River, Matthew T. Nichols

Graduate Student Theses, Dissertations, & Professional Papers

Enhanced loading of nitrogen (N) and phosphorus (P) is pervasive in rivers around the world. As a consequence of nutrient-rich conditions, many well-lit rivers experience severe algal blooms, which threaten water quality and aquatic habitats. Nutrient enrichment in the upper Clark Fork River, located in western Montana, has increased the productivity of benthic algae, Cladophora glomerata, which routinely forms extensive blooms during summer growing seasons (July – September). Addressing eutrophication in rivers begins with identifying the nutrients that contribute toward algal productivity. I deployed nutrient diffusing substrates (NDS), collected bi-weekly surface water samples, and conducted algal surveys at four …

Integrating Spatial Statistics And Decision Analysis For Wildfire Risk Mapping: A Case Study Of The Kootenai National Forest, Elijah Kordieh Mensah

Graduate Student Theses, Dissertations, & Professional Papers

Wildfire risk in the western United States has intensified in recent decades due to intersecting forces of climate change, systematic fire suppression, and expanding human settlement in fire-prone regions. This thesis presents a comprehensive spatial assessment of wildfire risk in the Kootenai National Forest (KNF) and its surrounding landscape in northwestern Montana, integrating wildfire likelihood, suppression difficulty, evacuation vulnerability, and building exposure into a unified spatial statistical framework. The research addresses a critical gap in spatial wildfire risk modeling by focusing on the need to assess fire threats in relation to local operational constraints and community vulnerabilities.
Drawing on geospatial …

Increasing Nature Connection And Decreasing Climate Change Distress In An Indigenous Population: The Development Of A Traditional Ecological Knowledge Intervention Framework, Olathe Antonio

Graduate Student Theses, Dissertations, & Professional Papers

Research suggests that nature connection is positively correlated with pro-environmental behaviors, concern for the climate, general wellbeing, and subjective levels of climate change distress. Few research studies have examined how to increase nature connectedness without increasing psychological distress. Such research is especially important for those who are disproportionately affected by climate change such as Indigenous Peoples. To address this discrepancy, we developed a culturally-grounded psychological intervention framework based in traditional ecological knowledge (TEK) for Indigenous populations and offered an adapted example for a Navajo population. The intervention was created in collaboration with TEK experts to conceptualize key psychologically-relevant TEK principles, …

Indigenous Placemaking, Colonial Placetaking, And Indigenous Data Sovereignty: Implications Of Environmental Data Collection On Tribal Lands, Paige Nizhonii Kya'iyo Johnson

Graduate Student Theses, Dissertations, & Professional Papers

This project investigates how Indigenous Data Sovereignty protocols apply to environmental data collected on Tribal lands by federal and state entities. The 2011 and 2014 flooding of the Missouri River and a need for further soil moisture data led the United States Army Corps of Engineers Omaha District to implement a Mesonet weather station monitoring network across the Upper Missouri River Basin (UMRB). Historically, the Army Corps of Engineers has not included Indigenous perspectives into their decision-making, and this has caused Tribes to be distrustful of partnerships with them. The UMRB monitoring network will span across the Upper Missouri River …

Predicting Language And Literacy Success In Children With And Without Developmental Language Disorder, Melissa Catherine Phelan

Graduate Student Theses, Dissertations, & Professional Papers

The purpose of this study was to investigate the roles of phonological, orthographic, and morphological awareness in predicting language and literacy success for children with developmental language disorder (DLD), DLD with dyslexia, and typically developing (TD) children. DLD, a prevalent yet underdiagnosed condition, is associated with significant psychosocial and academic challenges, including a roughly 50% likelihood of developing dyslexia. Current identification systems rely on inconsistent parent/teacher referrals, which miss approximately 60% of children with DLD and over 33% with dyslexia, limiting access to effective interventions. This study longitudinally examines whether kindergarten screenings of three metalinguistic skills—phonological, orthographic, and morphological awareness—predict …

Designing A Behavior-Supportive Literacy Block Using Ufli Phonics For First Grade Students With Behavioral Challenges, Julia Lynne Pugh

Graduate Student Theses, Dissertations, & Professional Papers

Students with behavioral challenges tend to struggle while engaging with structured academic instruction, particularly in foundational literacy blocks. In fact, in 2021, it was reported that 27.7% of children aged 3-17 struggle with a mental, behavioral, or developmental disorder. (Leeb et al., 2024) While programs such as UFLI (University of Florida Literacy Institute) offer highly effective phonics instruction, these programs typically lack embedded support for individuals struggling with self-regulation, attention, and task persistence. As a result, these students are presented with a higher risk of falling behind in their reading skills. In this project, the author aims to develop a …

Hormonal Decay After Death: Evaluating Dhea-S As A Potential Molecular Marker For Postmortem Estimation, Marisa Lydia Novak

Graduate Student Theses, Dissertations, & Professional Papers

Post-mortem interval (PMI) estimation is a central component of forensic anthropological investigation, but traditional methods for estimating time since death lose accuracy after ~72 hours. Recent research indicates that some steroid hormones may undergo predictable degradation patterns after death, offering potential use as biochemical clocks for PMI estimation beyond this threshold. Dehydroepiandrosterone (DHEA), and its sulfate form (DHEA-S) are of particular interest due to their metabolic stability, systemic influence, and association with trauma. This study investigates whether DHEA-S hormonal degradation patterns correlate with time elapsed since death and body temperature, evaluating their potential as biochemical PMI markers. 160 mL of …

From Plumes To People: Constraining The Emissions, Chemistry, And Health Impacts Of Western U.S. Smoke, Lixu Jin

Graduate Student Theses, Dissertations, & Professional Papers

Wildfires are a significant source of volatile organic compounds (VOCs) in the western U.S., emitting hundreds to thousands of species that play key roles in tropospheric oxidation, ozone production, and secondary organic aerosol formation. Many VOCs have only recently been identified and quantified in laboratory burning experiments, leaving uncertainties about their emission estimates from wildfires, their roles in plume OH oxidation chemistry, and their evolution during smoke aging. This dissertation aims to improve our understanding of the emissions, chemistry, and health impacts of VOCs in wildfire smoke using detailed in-situ measurements made during the summer 2018 Western Wildfire Experiment for …

Navigating The Currents: A Virtual Session On Reflection To Explore The Challenges And Lessons Learned During Online Medical Education In The Covid Pandemic., Smitha Elizabeth Kore, Miriam Archana Simon

Health Professions Education

Purpose: An online session in this study was conducted to teach the importance of reflections and discuss the challenges faced in online classes during the pandemic.

Methodology: An exploratory intervention-based study was conducted. A two-day Webex session introduced preclinical students to reflection. Day one included faculty-led sessions on reflection's importance in medicine. Whereas on day two there were presentation of selected student reflections and tips for future online learning from senior students. Quantitative and qualitative feedback was gathered through an online survey.

Statistical analysis included descriptive statistics, Cronbach’s alpha, Spearman’s correlation, simple linear regression and nearest neighbor analysis to …

Assessing Attitudes Towards Plagiarism Among Health Professionals: The Impact Of Scientific Writing Training, Humera Adeeb, Brekhna Jamil, Ihsan . Ullah

Health Professions Education

Purpose. Plagiarism is a common form of academic misconduct with damaging consequences to researchers and science. There is a need to explore attitudes towards plagiarism and adopt strategies to mitigate its effects.

Method. A cross-sectional analytical study was conducted among health professionals attending a course at Khyber Medical University. Data were collected from 1st September 2022 to 31st January 2023. Attitudes towards plagiarism questionnaire, with 29 items on a five-point Likert scale, was distributed on google forms to 240 participants. Age, gender, qualification, previously attended course on scientific writing and authoring an article were described as proportions. The unpaired t-test …

Modeling Ion-Specific Effects In Polyelectrolyte Brushes: A Modified Poisson-Nernst-Planck Model, William J. Ceely, Marina Chugunova, Ali Nadim, James D. Sterling

Business and Information Technology Faculty Research & Creative Works

Polyelectrolyte brushes consist of a set of charged linear macromolecules, each tethered at one end to a surface. An example is the glycocalyx which refers to hair-like negatively charged sugar molecules that coat the outside membrane of all cells. We consider the transport and equilibrium distribution of ions and the resulting electrical potential when such a brush is immersed in a salt buffer containing monovalent cations (sodium and/or potassium). The Gouy-Chapman model for ion screening at a charged surface captures the effects of the Coulombic force that drives ion electrophoresis and diffusion but neglects non-Coulombic forces and ion pairing. By …

Targeting Als: A Multi-Cellular Study On The Potential Therapeutic Role Of Azole-Based Compounds, Alisha R. Kennedy

Theses and Dissertations

Amyotrophic Lateral Sclerosis (ALS), also known as Lou Gehrig’s disease, is a progressive neurodegenerative disorder that leads to the degeneration of motor neurons, which control voluntary muscle movements such as eating, speaking, and breathing. As these neurons are damaged, voluntary movement ceases, leading to a fatal outcome. The survival time of ALS patients varies, ranging from a few months to up to 10 years. Chapter 1 of this thesis provides an overview of ALS, highlighting the role of transactive response DNA-binding protein 43 (TDP-43), a key feature in ALS and other neurodegenerative diseases. The aggregation of TDP-43, particularly due to …

Lights, Camera, Action: A Student's Perspective On Reimagining Film Production Opportunities, Ma'lon I. Lane

Theses and Dissertations

The pursuit of a crew job in the film industry is a multifaceted endeavor, offering various pathways that cater to diverse aspirations and circumstances. Central to this exploration is the question of whether attending a university and obtaining a degree in film is essential for success. I was determined to answer the following: What is the likelihood that a higher education in Film Production leads to more efficient job acquisition in the film industry? The project is considered narrative, originating from the personal quality of the research questions. I used my resources to gather qualitative data through interviews of film …

Online Parameter Adaptation Of Lqr Controllers Via Rls For Prosthetic Joint Control: Experimental Validation On A Quanser Qube-Servo 2 Platform, Cynthia Lopez-Jordan

Theses and Dissertations

This thesis develops and experimentally validates an online adaptive Linear Quadratic Regulator (LQR) control method for prosthetic joint systems using Recursive Least Squares (RLS)-based real-time parameter estimation on the Quanser QUBE-Servo 2 platform. Traditional LQR controllers assume a fxed system model, which limits adaptability and results in reduced tracking accuracy, poor robustness, and loss of optimal performance when applied to dynamically changing prosthetic joints, infuenced by load variations, user gait changes, and mechanical wear. Limited experimental validation exists for combining RLS with online LQR adaptation in prosthetic-like systems.

To address these limitations, an RLS-driven Adaptive LQR framework was implemented to …

From Curiosity To Controversy: American Perception Of Japanese Immigrants, 1853-1924, Chloe Elizabeth Curbelo

Theses and Dissertations

In the mid-nineteenth century, as the United States reached the Pacific through westward expansion, Asia emerged as a new stage for commerce and power. Japan, long isolated under the Tokugawa shogunate, became an object of American fascination when forced into relations. While early depictions cast Japan as an exotic yet uncivilized nation in need of Western guidance, Japan’s rapid modernization and the growing number of Japanese immigrants on the West Coast transformed curiosity into anxiety. My paper traces this evolution of perception from 1850 to 1924, arguing that shifting views of Japan and its people reflected America’s ethnocentric notions of …