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B. Hooks Elementary School’S Commitment To Inclusion: A Single Instrumental Case Study, Sadie Childs Cora Sep 2025

B. Hooks Elementary School’S Commitment To Inclusion: A Single Instrumental Case Study, Sadie Childs Cora

Educational Studies Dissertations

This study contributes to understandings of inclusive school reform by examining one school’s efforts to create a more equitable educational experience for all students. Despite widespread advocacy and legal mandates for inclusion, segregation of students with dis/abilities persists, along with significant achievement and opportunity gaps. Inclusion is one possible solution that is consistent with democratic values. This single instrumental case study was guided by three research questions focusing on how educators conceive of their efforts toward the inclusion of students with dis/abilities, changes made to support inclusion, and how educators define the success of inclusion efforts. Drawing on multiple data …


The Echo: September 15, 2025, Taylor University Sep 2025

The Echo: September 15, 2025, Taylor University

2025-2026 (Volume 113)

No abstract provided.


The Predilection Of Specific Diseases To Affect Different Sections Of The Esophagus, Monjur Ahmed Sep 2025

The Predilection Of Specific Diseases To Affect Different Sections Of The Esophagus, Monjur Ahmed

Division of Gastroenterology and Hepatology Faculty Papers

Certain esophageal diseases have a predilection for affecting different parts of the esophagus. This predilection is noted in our clinical practice as we investigate various esophageal diseases using multiple imaging and motility studies, as well as endoscopic procedures with biopsies. Clinical presentations vary with the stage and location of the disease. Clinicians can suspect certain diseases when a particular part of the esophagus is involved and then can perform appropriate investigations. Esophageal diseases with section involvement, with their symptomatology, diagnostic tests, and management, have been discussed in this narrative review. This review aims to revisit those diseases with a current …


A Neutrosophic Twofold Algebraic Framework For Modeling Uncertainty In University English Teachers' Development Competence In The Era Of Big Data, Chun Ning Sep 2025

A Neutrosophic Twofold Algebraic Framework For Modeling Uncertainty In University English Teachers' Development Competence In The Era Of Big Data, Chun Ning

Neutrosophic Sets and Systems

No abstract provided.


Educational Reforms In West Africa: Push And Pull Factors Analysis Of Student Mobility In Ghana, Nigeria, And The Ivory Coast, Aya Kouao Sep 2025

Educational Reforms In West Africa: Push And Pull Factors Analysis Of Student Mobility In Ghana, Nigeria, And The Ivory Coast, Aya Kouao

Journal of Comparative & International Higher Education

The interconnectivity of global capitals has led to the increasing movements of people across borders for political, social, and economic reasons. However, there has been an increased interest in countries located in the West. This paper focuses on the context of student mobility in West Africa, especially in the countries of the Ivory Coast, Ghana, and Nigeria for a better understanding of students’ attitudes toward pursuing their higher education in Western countries. Drawing from the push and pull theory, the purpose of this study is to explore the drivers of migration intentions among West African students while looking at the …


Perception Of The Toefl Prediction Test Of Tbi Students Batch 2021 Uin Datokarama Palu, Nur Aida Kasi Nanda, Ruslin Ruslin, Zuhra Zuhra, Naima Naima, Noval Afandy Sep 2025

Perception Of The Toefl Prediction Test Of Tbi Students Batch 2021 Uin Datokarama Palu, Nur Aida Kasi Nanda, Ruslin Ruslin, Zuhra Zuhra, Naima Naima, Noval Afandy

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

TOEFL Prediction is widely used in Indonesian higher education as a preparatory tool, yet students’ perceptions of the TOEFL test remain rarely explored. This qualitative case study involved ten English Education students (batch 2021) at UIN Datokarama Palu. Data were collected through semi-structured interviews and document analysis, then analyzed using Miles and Huberman’s interactive model. Four indicators emerged: readiness and confidence, test-taking experience, motivation, and perceived fairness. Students valued TOEFL Prediction as a diagnostic tool, but faced challenges such as anxiety, technical issues, and limited vocabulary. TOEFL Prediction functions both as formative assessment and a motivational catalyst for learning.


Educational Case: Diabetic Nephropathy, Emily W. Gripp, Joanna Chan Sep 2025

Educational Case: Diabetic Nephropathy, Emily W. Gripp, Joanna Chan

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Primary objective

The following fictional case is intended as a learning tool within the Pathology Competencies for Medical Education (PCME), a set of national standards for teaching pathology. These are divided into three basic competencies: Disease Mechanisms and Processes, Organ System Pathology, and Diagnostic Medicine and Therapeutic Pathology. For additional information, and a full list of learning objectives for all three competencies, see https://www.academicpathologyjournal.org/pcme.

Objective UTK5.4: Diabetic Nephropathy. Describe the pathogenesis of diabetic nephropathy and the associated clinicopathologic features. Competency 2: Organ System Pathology; Topic: Kidney (UTK); Learning Goal 5: Glomerular Disorders.

Secondary objective

Objective CHEM1.5: Renal Disorders. Discuss the …


The Progressive Changes In Posttraumatic Symptoms And Posttraumatic Growth Associated With Traumatic Incident Reduction, Joseph L. Mcclory Sep 2025

The Progressive Changes In Posttraumatic Symptoms And Posttraumatic Growth Associated With Traumatic Incident Reduction, Joseph L. Mcclory

Walden Dissertations and Doctoral Studies

Many millions of people are suffering from symptoms of posttraumatic stress disorder (PTSD). Just in the United States, 13 million Americans had a PTSD diagnosis as of 2020. Through the theoretical lens of catharsis, analysis of variance was used to examine whether traumatic incident reduction (TIR) is associated with changes in posttraumatic symptoms and posttraumatic growth by comparing pre, mid, and post-TIR PTSD Checklist for DSM-5 (PCL-5) and Posttraumatic Growth Inventory (PTGI) scores. Regression was also used to test whether post-PCL-5 scores accounted for variance in the post-PTGI scores. Ninety-seven TIR cases from the mental health agency between 2017 and …


Radiosensitization Of Intrahepatic Cholangiocarcinoma And Metastatic Disease In The Liver Using Microbubble Cavitation: Results From A Phase 2 Clinical Trial, Corinne E. Wessner, Tania Siu Xiao, Allison Chang, Jenny Liu, Weelic Chong, Ji-Bin Liu, Kristen Bradigan, Flemming Forsberg, Andrej Lyshchik, Patrick O'Kane, Scott W. Keith, Stephen R. Topper, Kevin Anton, John R. Eisenbrey Sep 2025

Radiosensitization Of Intrahepatic Cholangiocarcinoma And Metastatic Disease In The Liver Using Microbubble Cavitation: Results From A Phase 2 Clinical Trial, Corinne E. Wessner, Tania Siu Xiao, Allison Chang, Jenny Liu, Weelic Chong, Ji-Bin Liu, Kristen Bradigan, Flemming Forsberg, Andrej Lyshchik, Patrick O'Kane, Scott W. Keith, Stephen R. Topper, Kevin Anton, John R. Eisenbrey

Department of Radiology Faculty Papers

Ultrasound triggered microbubble destruction (UTMD) has been shown to sensitize tumors to radiation. This study evaluated the feasibility, safety, and efficacy of using UTMD for radiosensitization in participants with intrahepatic cholangiocarcinoma (ICC) and/or metastatic disease to the liver (MDL) receiving Yttrium-90 transarterial radioembolization (Y90-TARE). This was a prospective single-center IRB-approved clinical trial. Participants received four contrast ultrasound sessions: 1-2 weeks prior to Y90-TARE, 1-4 h post-treatment, and 1 week and 2 weeks post-Y90-TARE. The control group consisted of 1:1 nearest-neighbor propensity score matched historical controls participants that received Y90-TARE as part of standard-of-care. Safety was evaluated through general lab values, …


Pentingkah Pekerjaan Ibu Dalam Literasi Anak Di Indonesia?, Dien Amalina Nur Asrofi, Muhammad Salahudin Al Ayyubi Sep 2025

Pentingkah Pekerjaan Ibu Dalam Literasi Anak Di Indonesia?, Dien Amalina Nur Asrofi, Muhammad Salahudin Al Ayyubi

Jurnal Ekonomi Kependudukan dan Keluarga

This study aims to analyze the impact of maternal employment status and job type on children's literacy skills in Indonesia, using data from the 2023 National Socioeconomic Survey (SUSENAS) and a binary logistic regression approach. The results show that working mothers, particularly those in the formal sector, are positively and significantly correlated with children's literacy, especially in non-poor households. These findings highlight the important role of maternal employment contribution in strengthening educational investment within families. In contrast, among poor households, formal employment of mothers does not significantly influence children's literacy, indicating that economic constraints remain a major barrier. In addition, …


Discovering The Doctrine Of Discovery, Douglas Lind Sep 2025

Discovering The Doctrine Of Discovery, Douglas Lind

Montana Law Review

No abstract provided.


A Reciprocity: Off-Reservation Treaty Hunting Rights, Sarah K. Yarlott Sep 2025

A Reciprocity: Off-Reservation Treaty Hunting Rights, Sarah K. Yarlott

Montana Law Review

No abstract provided.


Emphasizing A Student-Centered Process: Open Pedagogy Course Assessments Across Disciplines, Angela M. Mcgowan-Kirsch, Kelly Soczka Steidinger, Christina R. Hilburger, Heather J. Hether, Jean Hertzberg, Amanda Lohiser Sep 2025

Emphasizing A Student-Centered Process: Open Pedagogy Course Assessments Across Disciplines, Angela M. Mcgowan-Kirsch, Kelly Soczka Steidinger, Christina R. Hilburger, Heather J. Hether, Jean Hertzberg, Amanda Lohiser

Milne Open Textbooks

Emphasizing a Student-Centered Process: Open Pedagogy Course Assessments Across Disciplines showcases how Open Educational Practices (OEP) empower students as active contributors to knowledge creation. Grounded in constructivist principles, this collection highlights student-centered assessments—from collaborative course design and renewable assignments to generative artificial intelligence—that foster critical thinking, active learning, and inclusivity. Through practical examples and reflective discussions, the book provides educators with actionable strategies to integrate open pedagogical practices across disciplines while addressing challenges such as institutional support, professional development, and resource accessibility.


Early Definitive Fusion Versus Magnetic Growing Rods In "Tweeners": What Do Parents Prefer?, Sylvia Culpepper, Ravi Rajendra, Brett Lullo, Anthony A. Catanzano, Tyler C. Mcdonald, R Carter Clement Sep 2025

Early Definitive Fusion Versus Magnetic Growing Rods In "Tweeners": What Do Parents Prefer?, Sylvia Culpepper, Ravi Rajendra, Brett Lullo, Anthony A. Catanzano, Tyler C. Mcdonald, R Carter Clement

School of Medicine Faculty Publications

INTRODUCTION: In treating early-onset scoliosis (EOS), pediatric orthopaedic surgeons and families must often choose between growth-friendly interventions such as magnetically controlled growing rods (MCGR) and early definitive posterior spinal fusion (PSF). While each strategy has associated pros and cons, little is understood about the specific preferences of patients' families. Addressing potential nuances in these preferences is crucial for meaningful shared decision-making (SDM). To better understand the factors influencing family decisions, we conducted a choice-based conjoint (CBC) analysis to explore parents' preferences for PSF and MCGR as treatment options. METHODS: A survey was developed using Sawtooth Software (Lighthouse Studio version 9.2.0) …


Biophobia And Pet Ownership: Can Having A Pet Mitigate Our Fear And Aversion Towards Nature?, Alexia Barrable Sep 2025

Biophobia And Pet Ownership: Can Having A Pet Mitigate Our Fear And Aversion Towards Nature?, Alexia Barrable

The International Journal of Ecopsychology (IJE)

Biophobia, which we defined as fear, disgust, or aversion toward natural environments or organisms, has received little empirical attention when compared to biophilia, despite its implications for human and planetary wellbeing. This mixed-methods study explores the relationship between pet ownership, biophobia, and nature connectedness among 373 English- speaking adults. Quantitative findings reveal that (M = 26.4) exhibit significantly lower levels of biophobia than non-pet owners (M = 30.1; U = 5179, Z = -2.95, p = .002, R = 0.27), although no significant differences in nature connectedness were found across pet ownership (U = 7033, Z = 0.31, p = …


From Weberian Rationalization To Javascript Components: Modularism In Academic Library Software, Mark E. Eaton Sep 2025

From Weberian Rationalization To Javascript Components: Modularism In Academic Library Software, Mark E. Eaton

Publications and Research

This paper considers modular approaches to building library software and situates these practices within the context of the rationalizing logics of modern programming. It briefly traces modularism through its elaboration in the social sciences, in computer science and ultimately describes it as it is deployed in contemporary academic libraries. Using the methodology of a case study, we consider some of the very tangible and pragmatic benefits of a modular approach, while also touching upon some of the broader implications. We find that the modularism is deeply integrated into modern software practice, and that it can help support the work of …


Setd2 Suppresses Tumorigenesis In A Krasg12c-Driven Lung Cancer Model, And Its Catalytic Activity Is Regulated By Histone Acetylation, Ricardo J Mack, Natasha M Flores, Geoffrey C Fox, Hanyang Dong, Metehan Cebeci, Simone Hausmann, Tourkian Chasan, Jill M Dowen, Brian D Strahl, Pawel K Mazur, Or Gozani Sep 2025

Setd2 Suppresses Tumorigenesis In A Krasg12c-Driven Lung Cancer Model, And Its Catalytic Activity Is Regulated By Histone Acetylation, Ricardo J Mack, Natasha M Flores, Geoffrey C Fox, Hanyang Dong, Metehan Cebeci, Simone Hausmann, Tourkian Chasan, Jill M Dowen, Brian D Strahl, Pawel K Mazur, Or Gozani

Faculty, Staff and Student Publications

Histone H3 trimethylation at lysine 36 (H3K36me3) is a key chromatin modification that regulates fundamental physiological and pathological processes. In humans, SETD2 is the only known enzyme that catalyzes H3K36me3 in somatic cells and is implicated in tumor suppression across multiple cancer types. While there is considerable crosstalk between the SETD2-H3K36me3 axis and other epigenetic modifications, much remains to be understood. Here, we show that Setd2 functions as a potent tumor suppressor in a KRASG12C-driven lung adenocarcinoma (LUAD) mouse model, and that acetylation enhances SETD2 in vitro methylation of H3K36 on nucleosome substrates. In vivo, Setd2 ablation accelerates lethality in …


Bayesian Clustering Prior With Overlapping Indices For Effective Use Of Multisource External Data, Xuetao Lu, J Jack Lee Sep 2025

Bayesian Clustering Prior With Overlapping Indices For Effective Use Of Multisource External Data, Xuetao Lu, J Jack Lee

Faculty, Staff and Student Publications

The use of external data in clinical trials offers numerous advantages, such as reducing enrollment, increasing study power, and shortening trial duration. In Bayesian inference, information in external data can be transferred into an informative prior for future borrowing (i.e. prior synthesis). However, multisource external data often exhibits heterogeneity, which can cause information distortion during the prior synthesizing. Clustering helps identifying the heterogeneity, enhancing the congruence between synthesized prior and external data. Obtaining optimal clustering is challenging due to the trade-off between congruence with external data and robustness to future data. We introduce two overlapping indices: the overlapping clustering index …


Optimizing Ai-Driven Digital Resources In Vocational English Learning Using Plithogenic N-Superhypergraph Structures For Adaptive Content Recommendation, Liang Zhang, Min Huang, Fenghua Li Sep 2025

Optimizing Ai-Driven Digital Resources In Vocational English Learning Using Plithogenic N-Superhypergraph Structures For Adaptive Content Recommendation, Liang Zhang, Min Huang, Fenghua Li

Neutrosophic Sets and Systems

No abstract provided.


Neutrosophic Sets And Systems, Vol. 88,2025, Florentin Smarandache, Mohamed Abdel-Basset, Maikel. Leyva Vazquez Sep 2025

Neutrosophic Sets And Systems, Vol. 88,2025, Florentin Smarandache, Mohamed Abdel-Basset, Maikel. Leyva Vazquez

Neutrosophic Sets and Systems

No abstract provided.


Scoping Review Of Sexual And Gender Minority Health Research In Ireland, John P. Gilmore, Tonda L. Hughes, Sean Kearns, Laurie A. Drabble, Diarmuid Stokes, Siobhán D. Thomas, Chris Noone, Lauren Bochicchio Sep 2025

Scoping Review Of Sexual And Gender Minority Health Research In Ireland, John P. Gilmore, Tonda L. Hughes, Sean Kearns, Laurie A. Drabble, Diarmuid Stokes, Siobhán D. Thomas, Chris Noone, Lauren Bochicchio

Faculty Research, Scholarly, and Creative Activity

Aim: To map existing sexual and gender minority (SGM) health research in Ireland, identify gaps in literature and outline priorities for future research and healthcare. SGM is an umbrella term that includes people who identify as lesbian, gay, bisexual, transgender, queer or intersex and is sometimes abbreviated as LGBTQI+. Design: A scoping review of peer-reviewed studies published between 2014 and 2024. Methods: The review followed Joanna Briggs Institute (JBI) guidelines and PRISMA-ScR framework for scoping reviews. Articles were identified through systematic database searches and screened independently by reviewers. Data Sources: PubMed, PsycINFO, CINAHL and Embase were searched for articles published …


Ptsd Symptoms Moderate Predictors Of Psychophysiological Arousal During Fear Inhibition: Evidence From A Fear, Reward, And Neutral Discrimination Task, Jacklynn M. Fitzgerald, E. Kate Webb, Kaley Davis, Meghan Bennett, Tristan Benjamin, Boiana Pegau, Susan Sangha Sep 2025

Ptsd Symptoms Moderate Predictors Of Psychophysiological Arousal During Fear Inhibition: Evidence From A Fear, Reward, And Neutral Discrimination Task, Jacklynn M. Fitzgerald, E. Kate Webb, Kaley Davis, Meghan Bennett, Tristan Benjamin, Boiana Pegau, Susan Sangha

Psychology Faculty Research and Publications

The ability to distinguish between threatening, rewarding, and neutral cues is adaptative and crucial for survival. However, individuals with posttraumatic stress disorder (PTSD) often show poor knowledge of cue contingencies and heightened fear responses even in the presence of cues that signify safety, potentially due to atypical perceptions of neutral cues. We investigated whether perceiving neutral cues as more rewarding or threatening influences conditioned inhibition of fear and whether PTSD symptoms moderate this relationship. Trauma-exposed adults (N = 84; 64 % female; 76 % non-Hispanic white) completed a Fear, Reward, and Neutral Discrimination (FRND) Task involving geometric shapes paired with …


2025 Latinx Heritage Month, Illinois State University, Latin American And Latino/A Studies Program Sep 2025

2025 Latinx Heritage Month, Illinois State University, Latin American And Latino/A Studies Program

Latinx Heritage Month Posters

Schedule of events for Illinois State University's Latinx Heritage Month celebration


Digital Healthcare Interventions To Support Parents With Acutely Ill Children At Home: A Systematic Review., Matt Carey, Jane Peters, Anna Chick, Bernie Carter, Lucy Bray, Damian Roland, Sarah Neill Sep 2025

Digital Healthcare Interventions To Support Parents With Acutely Ill Children At Home: A Systematic Review., Matt Carey, Jane Peters, Anna Chick, Bernie Carter, Lucy Bray, Damian Roland, Sarah Neill

School of Nursing and Midwifery

Background Short lived acute illness in children is common, yet their parents often feel uncertain about recognising signs symptoms of acute illness and knowing when to seek medical intervention. This has led to seeking unscheduled or delayed support. Digital and mobile technologies are being used to support individuals with healthcare needs, known as digital health interventions. Parents have access to digital health interventions that provide information regarding children’s health, yet there is limited exploration of how these are used to support decision-making when caring for acutely ill children. This systematic review was undertaken to explore digital interventions to support parents …


Searching For Meaning In A Chaotic World: An Analysis Of Tina Howe’S Birth And After Birth, Ali Qasim Radhee, Sabah Atallah Khalifa Ali Sep 2025

Searching For Meaning In A Chaotic World: An Analysis Of Tina Howe’S Birth And After Birth, Ali Qasim Radhee, Sabah Atallah Khalifa Ali

Alustath Journal for Human and Social Sciences

This paper deals with Tina Howe’s play Birth and After Birth as an absurd work. The aim is to harbor on meaning out of the chaotic life of the characters in the play. Topics like motherhood, archetypes, domestic abuse and social bias are discussed in this paper. The implementations of these topics help in shedding light on the motives behind the characters’ actions and consequently reach out to a better understanding of their behaviors and those of the American people in general.

Howe’s work reflects the traditional life of a stereotypical American family during the 1970s, a life that is …


Concentration-Dependent Responses Of C. Reinhardtii To Silver Ions: Hormetic Response In Growth And Reduction Of Motility, Hemanta Pradhan, Arpan Poudel, Diksha Shrestha, Ariel Rogers, Michael Stewart, Amani Jereb, Jack Harper, Ming Li, Wen Zhang, Jingyi Chen, Yong Wang Sep 2025

Concentration-Dependent Responses Of C. Reinhardtii To Silver Ions: Hormetic Response In Growth And Reduction Of Motility, Hemanta Pradhan, Arpan Poudel, Diksha Shrestha, Ariel Rogers, Michael Stewart, Amani Jereb, Jack Harper, Ming Li, Wen Zhang, Jingyi Chen, Yong Wang

Physics Faculty Publications and Presentations

Elevated levels of silver in aquatic environments arising from widespread use of silver nitrate and silver nanoparticles in different sectors of industry and medicine pose significant biophysical challenges to aquatic microorganisms. Despite extensive toxicity studies of silver on bacteria and microbial communities, its influence on other aquatic microorganisms, such as microalgae, remains poorly understood. In this study, we investigated the biophysical response of C. reinhardtii microalgae to silver ion exposure in terms of their population growth dynamics, chlorophyll content, and swimming motility. We found that silver ions at different concentrations (from 0.29 to 1.18 mu\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} …


Steiner And Jung On Esoteric Knowledge, Katherine T. Ziemke Mst, Jd, Phd Candidate Sep 2025

Steiner And Jung On Esoteric Knowledge, Katherine T. Ziemke Mst, Jd, Phd Candidate

Journal of Conscious Evolution

For centuries, humanity has been accumulating complex knowledge, systematically building increasingly intricate mathematical and scientific models by accruing predictable circumstances or facts, often designated as theories or laws. This essay proposes that there is a hidden well-spring of ideas or knowledge beyond the methodical accumulation of such mathematical and scientific knowledge, from whence the source that inspired the basis of this knowledge might have originated. Carl Gustav Jung and Rudolf Steiner believe there is an abundance of such knowledge that lies hidden in plain sight and available for a vast expansion of human awareness. In this essay, I take a …


Chimes: September 15, 2025, Calvin University Sep 2025

Chimes: September 15, 2025, Calvin University

Chimes

A historical moment: Calvin hosts first-ever football game on campus by Zach Kern & Hendrik Beelen

From 40 to 62: Honors Scholars' biggest cohort yet by Sarah Sparling

Riding the Rapid: Explaining the Wave Card price increase by Ian Tjoelker

She's "complicated": Jane Austin film festival marks the author's 250th birthday by Ellery Johnson

Strength in spirit: The faith-fueled fitness journey of Macy Gerig by Ella Vanden Berg

Native Plant Sale aims to build biodiversity and relationships by Maya Oeverman


Transcultural Competence And Empathy In Language Education: Imagining The Unimaginable, Michiko Uryu Sep 2025

Transcultural Competence And Empathy In Language Education: Imagining The Unimaginable, Michiko Uryu

Faculty Research, Scholarly, and Creative Activity

The 2007 MLA report offered guidelines and competencies for foreign language majors at American universities in the age of globalization. The notion of translingual and transcultural competence suggests that today’s foreign language education is not merely a matter of language acquisition, but humanistic learning. The ultimate goal for foreign language learners should be to gain alternative ways of seeing the world, namely, “imagining the unimaginable” (Ozick, 1987). This underscores the central importance of empathy in foreign language education. Yet the true challenge lies in how to assess such an abstract concept. Even eighteen years since its initial publication, the report …


Developmental Transformations Of Purkinje Cells Tracked By Dna Electrokinetic Mobility, Cheryl Brandenburg, Garrett W Crutcher, Andrea J Romanowski, Sarah G Donofrio, Lita R Duraine, Richard N A Owusu-Mensah, Benjamin H Cooper, Izumi Sugihara, Gene J Blatt, Roy V Sillitoe, Alexandros Poulopoulos Sep 2025

Developmental Transformations Of Purkinje Cells Tracked By Dna Electrokinetic Mobility, Cheryl Brandenburg, Garrett W Crutcher, Andrea J Romanowski, Sarah G Donofrio, Lita R Duraine, Richard N A Owusu-Mensah, Benjamin H Cooper, Izumi Sugihara, Gene J Blatt, Roy V Sillitoe, Alexandros Poulopoulos

Duncan NRI Faculty and Staff Publications

Brain development begins with neurogenesis in progenitor zones and ends with expansive, intricately-patterned cellular diversity in the adult brain. We took advantage of bioelectric interactions between DNA and embryonic tissue to perform "stereo-tracking," a developmental targeting strategy that differentially labels cells at different depths within progenitor zones. This 3D labeling was achieved by delivery of plasmids with distinct electrokinetic mobilities in utero. We applied stereo-tracking with light sheet imaging in the cerebellum and identified that Purkinje cells follow embryonically committed developmental trajectories, linking distinct progenitor zone subfields to the mature topography of the cerebellar cortex. We additionally identified an unexpected …