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Full-Text Articles in Entire DC Network
Fast Radio Bursts From The Nonlinear Evolution Of Low Frequency Plasma Waves In The Magnetosphere Of Magnetars, Dominic Bernardi
Fast Radio Bursts From The Nonlinear Evolution Of Low Frequency Plasma Waves In The Magnetosphere Of Magnetars, Dominic Bernardi
Arts & Sciences Graduate Student Theses and Dissertations
Fast radio bursts (FRBs) are millisecond-duration radio bursts with applications as cosmic probes. However, their physical origins remain largely unknown. Magnetars are natural candidates for producing FRBs due to their compact size, extremely strong magnetic fields, and intrinsically dynamic nature. In this thesis, I develop a model for FRB production grounded in both a concrete triggering mechanism and a viable emission process. This process begins by launching low-frequency plasma waves into the magnetosphere. I show that energetic fast magnetosonic waves can naturally be produced in the magnetosphere, independent of how these waves are launched. Regardless of whether these waves are …
Sirt1 And Ifi16 Cooperatively Regulate Hbv Replication Via Epigenetic Modulation And Innate Immune Activation, Zahra Zahid Piracha, Umar Saeed, Neelam Abid, Syed Shahan Gilani, Hafiz Ahmad Bilal Akhtar Chughtai, Areesha Wasti, Muhammad Waseem, Dilber Uzun Ozsahin, Andromeda M. Nauli, Surya M. Nauli
Sirt1 And Ifi16 Cooperatively Regulate Hbv Replication Via Epigenetic Modulation And Innate Immune Activation, Zahra Zahid Piracha, Umar Saeed, Neelam Abid, Syed Shahan Gilani, Hafiz Ahmad Bilal Akhtar Chughtai, Areesha Wasti, Muhammad Waseem, Dilber Uzun Ozsahin, Andromeda M. Nauli, Surya M. Nauli
Pharmacy Faculty Articles and Research
Background
Hepatitis B virus (HBV) establishes persistent infection through the formation of a covalently closed circular DNA (cccDNA) minichromosome, which enables immune evasion and sustained viral replication. While SIRT1, IFI16, and STING are known to modulate antiviral pathways, their cooperative role in regulating HBV transcription and host immunity remains poorly defined.
Methods
We investigated the functional interplay between SIRT1, IFI16, and STING using confocal microscopy, chromatin immunoprecipitation (ChIP), gene silencing, and quantitative PCR in HBV-replicating cell models. Interactions with HBV cccDNA and effects on viral transcription and interferon-stimulated gene (ISG) expression were analyzed. Northern blotting and RT-qPCR were used to …
From Athens To Jerusalem: Platonism, Richard Owen, And The Road Not Taken In Biology, Michael A. Flannery
From Athens To Jerusalem: Platonism, Richard Owen, And The Road Not Taken In Biology, Michael A. Flannery
Libraries Professional Work
Tertullian provocatively asked (circa 200 AD), “What indeed has Athens to do with Jerusalem? What concord is there between the Academy and the Church?” The relationship between formal philosophy and religion has been a contentious battleground ever since. It has historically come into sharpest focus in biology generally, and evolutionary theory specifically. Charles Darwin clearly won the day in the short-term. His evolutionary functionalism looked like an inglorious abandonment of time-honored metaphysical realism for positivist empirical reductionism with a concomitant horizontalization of perspective that has secularized and flattened the intellectual landscape. But more recently the rise of evo-devo (evolutionary developmental …
Assessing The Effectiveness Of Wetland Reserve Easements As Habitat For Birds In The Mississippi Alluvial Valley Of Eastern Arkansas, Colin K. Dobson
Assessing The Effectiveness Of Wetland Reserve Easements As Habitat For Birds In The Mississippi Alluvial Valley Of Eastern Arkansas, Colin K. Dobson
Student Theses and Dissertations
Globally, wetland habitats suffered major declines during the 20th century. In the 1990s, the United States government created the Wetland Reserve Program (later consolidated into the Agricultural Conservation Easement Program), to combat the loss of wetlands. Despite success in attracting landowners, minimal monitoring of properties enrolled into the program (easements) has occurred. In this study, we monitored avian communities at easements, compared avian diversity at easements to agriculture and bottomland forests, and quantified microhabitat, site-specific, and surrounding landscape features at easements. We also studied the demographic rates of an indicator species of bottomland forests, the Prothonotary Warbler (Protonotaria citrea) at …
Social Media And Health Information Sharing In Nigeria: Trends In Weight Loss And Cosmetic Procedures, Victoria Ochanya Agbo
Social Media And Health Information Sharing In Nigeria: Trends In Weight Loss And Cosmetic Procedures, Victoria Ochanya Agbo
Student Theses and Dissertations
This study examines the role of social media in facilitating (mis)information on weight loss and cosmetic surgeries in Nigeria. Utilizing the Elaboration Likelihood Model (ELM), the study examines information shared by influencers, audience engagements with such messages, and the factors influencing audience receptivity of the messages. Using a qualitative design, a thematic analysis was conducted on influencer posts and audience responses to such posts across major social media platforms. The findings show that influencers frequently framed cosmetic surgery and weight loss as aspirational lifestyle choices and downplayed the medical risks using means such as testimonials, visual transformations, and authenticity cues. …
Quality Improvement Project: Impact Of Formalized Debriefing On Correctional Health Emergency Response, Angel Woody
Quality Improvement Project: Impact Of Formalized Debriefing On Correctional Health Emergency Response, Angel Woody
Doctor of Nursing Practice Projects
Structured debriefing following medical emergencies has been identified as an evidence-based strategy to improve communication, reflective learning, and staff confidence within high-acuity healthcare settings. Inconsistent debriefing practices within correctional healthcare environments contributed to a gap in emergency response support and staff preparedness. The purpose of this quality improvement project was to evaluate whether implementation of a formalized debriefing protocol following medical emergencies impacted staff confidence during emergency response situations within a correctional healthcare setting. The project was guided by Bandura’s Self-Efficacy Theory and Lewin’s Change Theory. A quantitative one-group pretest-posttest quality improvement design was implemented in a county correctional healthcare …
The Transitional Liver Clinic: Study Protocol For A Stepped-Wedge Cluster Randomized Trial, Lindsay Yoder, Elliot B. Tapper, Richard S. Kalman, Mary Rinella, Nicole Bendin, Manisha Verma, Francis Pike, Naga Chalasani, Neha Nalumasu, Eric S. Orman
The Transitional Liver Clinic: Study Protocol For A Stepped-Wedge Cluster Randomized Trial, Lindsay Yoder, Elliot B. Tapper, Richard S. Kalman, Mary Rinella, Nicole Bendin, Manisha Verma, Francis Pike, Naga Chalasani, Neha Nalumasu, Eric S. Orman
Einstein Health Papers
BACKGROUND: Patients with complications of advanced liver disease experience high rates of hospital readmission after discharge. Transitional care models have improved outcomes in other chronic conditions, but their efficacy in liver disease care remains uncertain. This randomized trial evaluates the effectiveness of a tailored transitional care model-the Transitional Liver Clinic (TLC)-in reducing readmissions, improving quality of life, and enhancing patient satisfaction for patients with advanced liver disease.
METHODS: This is a 45-month, stepped-wedge cluster randomized trial enrolling up to 1000 patients with advanced liver disease across 4 academic medical centers. Each site transitions from usual care to the TLC intervention …
Decent Work In The Platform Economy Convention, 2026: A Preliminary Analysis, Valerio De Stefano
Decent Work In The Platform Economy Convention, 2026: A Preliminary Analysis, Valerio De Stefano
ILO Decent Work in the Platform Economy Convention
This paper provides a preliminary analysis of the Decent Work in the Platform Economy Convention, 2026 (No. 193), the first international labour standard devoted specifically to platform work. It examines the Convention’s scope, substantive protections, and principal interpretive questions, arguing that its significance lies in the cumulative architecture through which platform work is brought within the reach of international labour standards.
Particular attention is given to the Convention’s broad personal scope. Self-employed platform workers are generally included throughout the instrument, while specific provisions permit protections to be adapted to different employment statuses without excluding workers outside an employment relationship. …
Perceptions Of Trauma-Informed Trained Campus Leadership, Shelly Hardin
Perceptions Of Trauma-Informed Trained Campus Leadership, Shelly Hardin
Student Theses and Dissertations
The Centers for Disease Control and Prevention define adverse childhood experiences (ACEs) as potentially traumatic experiences that occur in a person’s life between birth and age 17. According to the Substance Abuse and Mental Health Services Administration (2024), more than two thirds of children experience at least one traumatic event by the age of 16, and this is likely an underestimate, as this percentage represents only those cases reported. The physical, emotional, and neurological impact of traumatic experiences can be detrimental to a child’s education, often contributing to learning deficits and behavioral issues. School leaders have the responsibility of creating …
Vibrations Of Tapered Beam Via The Exterior Matrix Method, Simranjit Kaur
Vibrations Of Tapered Beam Via The Exterior Matrix Method, Simranjit Kaur
Student Theses and Dissertations
Cell phone towers, utility poles, and traffic signal poles all use hollow, tapered beams as their main structural element. Because these structures are tall and exposed to wind forces, understanding their vibration behavior is important for ensuring stability and safety. We will use the Exterior Matrix Method to analyse a single beam, which can be used to analyse compound structures involving tapered beams. First, we find the system of four equations satisfied by the tapered beam, which can be converted to a 4 x 4 matrix. Then we find the exterior matrix, which is a 6 x 6 matrix, corresponding …
The Implementation Of Restorative Practices Through The Don’T Suspend Me! Professional Development, Bethanie Hale
The Implementation Of Restorative Practices Through The Don’T Suspend Me! Professional Development, Bethanie Hale
Student Theses and Dissertations
The purpose of this qualitative single case study was to examine how participation in the Don’t Suspend Me! (2022) professional development influenced teachers’ beliefs about discipline and their implementation of restorative practices within one public school district. Guided by Guskey’s (2002) Model of Teacher Change, this study sought to answer the research question: How does participation in the Don’t Suspend Me! (2022) professional development influence teachers’ implementation of restorative disciplinary practices and their beliefs about discipline within one public school district? Data were collected through a teacher questionnaire, individual interviews, and researcher reflexive journaling, with trustworthiness supported through triangulation, member …
Influences Of Transformational Leadership Mixed With Contemporary Practices Among High School Coaches: A Qualitative Exploratory Study, Kylene M. Brewton
Influences Of Transformational Leadership Mixed With Contemporary Practices Among High School Coaches: A Qualitative Exploratory Study, Kylene M. Brewton
Student Theses and Dissertations
This qualitative study examined factors influencing transformational leadership (TL) among high school coaches. Although previous research shows that TL improves athlete performance, emotional development, and team cohesion, little attention has been paid to how TL skills develop in secondary school sports, specifically to be used by coaching staff. Using Bass and Avolio’s four “I’s” framework—idealized influence, inspirational motivation, intellectual stimulation, and individualized consideration—the study explored the impact of gender, coaching experience, sport coached, and mentorship on TL practices. Ten high school coaches from the mid-southern United States participated in three rounds of interviews using the Differentiated Transformational Leadership Inventory (DTLI). …
African American Genealogists And Family Historians: Perceptions, Motivations, And Learning Experiences, Vernita A. Morgan
African American Genealogists And Family Historians: Perceptions, Motivations, And Learning Experiences, Vernita A. Morgan
Student Theses and Dissertations
In this qualitative phenomenological study, the motivations, learning experiences, challenges, and triumphs of African American genealogists and family historians will be revealed. The purpose of this study is to bridge the existing gap in the literature regarding the experiences of these individuals. The interconnectedness of self-directed learning and self-determination theory will demonstrate how these genealogists approach researching their ancestors, who were both enslaved and free people of color. Themes that arise from feedback from interviews will be used to offer answers to the research questions.
An Analysis Of How Educators’ Attitudes Toward Individuals With Intellectual Disabilities Impact Assessment Scores Of Students With Learning Disabilities, Felicia Baty Goings
An Analysis Of How Educators’ Attitudes Toward Individuals With Intellectual Disabilities Impact Assessment Scores Of Students With Learning Disabilities, Felicia Baty Goings
Student Theses and Dissertations
This study analyzed how educators’ attitudes toward individuals with intellectual disabilities impact summative assessment scores of students with learning disabilities. The research problem addressed in this study was the increase in the number of special education students receiving inclusive instruction who scored at Levels 3 or 4 on the Arkansas Teaching and Learning Assessment System (ATLAS) mathematics and English Language Arts (ELA) summative assessment. The findings are relevant to educators and education stakeholders wanting to improve inclusion practices. The purpose of this correlational quantitative study was to determine whether there was a significant difference in students’ summative assessment scores and …
Educator Perceptions Of The Impact Of The Avid Program On Minority Students’ School Achievement, Genia Smith
Educator Perceptions Of The Impact Of The Avid Program On Minority Students’ School Achievement, Genia Smith
Student Theses and Dissertations
While Hispanic and African American college enrollment rates have increased over the past 20 years, minority students continue to face structural and educational barriers that make it more challenging for them to prepare for and gain entry to college. The Advancement Via Individual Determination (AVID) program is a college preparatory initiative designed to provide additional academic and social support for historically disadvantaged students. Guided by a socio-cultural theoretical framework, this mixed-methods survey-based study explored the perceptions of 10 administrators and 10 teachers in a Southeast region school district regarding the impacts of AVID on minority students’ achievement. The findings revealed …
Impact Of Expanded Core Curriculum On College And Job Placement For The Blind And Visually Impaired, Susan Drake
Impact Of Expanded Core Curriculum On College And Job Placement For The Blind And Visually Impaired, Susan Drake
Student Theses and Dissertations
Students who are blind or visually impaired face persistent barriers in postsecondary education and employment, despite access to academic instruction and legally mandated supports. Although academic accommodations are often provided, less is known about how instruction in nonacademic skill areas influences long-term outcomes. . This study addressed a critical gap by examining the perceived impact of Expanded Core Curriculum instruction on college readiness, employment attainment, and adult independence for blind or visually impaired high school graduates. The purpose of this qualitative study was to explore how former students described their access to, understanding of, and benefit from Expanded Core Curriculum …
Cap1 (Cyclase-Associated Protein 1) Mediates The Camp Regulation Of Adhesion And Proliferation Of Schwann Cells In The Peripheral Nervous System, Jarna Karmoker
Cap1 (Cyclase-Associated Protein 1) Mediates The Camp Regulation Of Adhesion And Proliferation Of Schwann Cells In The Peripheral Nervous System, Jarna Karmoker
Student Theses and Dissertations
The peripheral nervous system relays signals between the central nervous system and the rest of the body, and peripheral nerve injuries and neuropathies can cause permanent neurological deficits. Schwann cells (SCs) are glial cells that myelinate neurons, which is essential for neuronal function and nerve repair. CAP1 (Cyclase-Associated Protein 1) is best characterized as an actin-regulating protein, while it also regulates other cellular processes, including matrix adhesion, migration, and proliferation. We discovered that cAMP-induced CAP1 dephosphorylation regulates relevant cell functions. While cAMP is also important for SC function and myelination, the underlying mechanisms remain largely unknown. We silenced CAP1 in …
Analysis Of Inclusive Practices Using A Co-Teaching Model On English Learners With Disabilities, Shawna Shields
Analysis Of Inclusive Practices Using A Co-Teaching Model On English Learners With Disabilities, Shawna Shields
Student Theses and Dissertations
This qualitative phenomenological study explored the lived experiences of general and special education teachers who implemented co-teaching as a model of inclusive instruction for English Learners (ELs) with disabilities in a suburban middle school district. Grounded in Cook and Friend’s (1995) co-teaching framework and informed by sociocultural theory, the study examined how teachers collaborated, negotiated roles, and balanced language development with disability-related supports. Data were collected through semi-structured interviews, focus groups, and a Co-teaching Collaboration Survey administered to 12 educators. Thematic analysis revealed that trust and parity support effective co-teaching partnerships, that language-forward scaffolds and interaction-based strategies promote engagement, and …
Comparative Effectiveness Of Augmentation, Switching, And Dose Escalation After Inadequate Antidepressant Response In Medicare Beneficiaries, Chih-Ting (Tim) Lai, Jingjing Qian, Cherry Jackson, Kimberly Garza, Jingyi Zheng, Richard Chapman, Surachat Ngorsuraches
Comparative Effectiveness Of Augmentation, Switching, And Dose Escalation After Inadequate Antidepressant Response In Medicare Beneficiaries, Chih-Ting (Tim) Lai, Jingjing Qian, Cherry Jackson, Kimberly Garza, Jingyi Zheng, Richard Chapman, Surachat Ngorsuraches
Podiums
Objective: To evaluate the comparative effectiveness of treatment augmentation, switching, or dose escalation for inadequate treatment response among patients living with depression. Methods: We conducted a retrospective cohort study using data from the Medicare Current Beneficiary Survey(MCBS; 2016-2022). We identified beneficiaries with depression who had both survey responses and had at least one antidepressant claim record 6 months before the outcome assessment. We excluded patients diagnosed with bipolar disorder, schizophrenia, and other psychotic disorders. Patients with evidence of treatment resistance, determined by using 3 or more antidepressants, or any antipsychotics at baseline, were also excluded. The primary outcome was the …
American Sign Language Recognition And Analysis Using Deep Learning, Saurabh Kumar Soni
American Sign Language Recognition And Analysis Using Deep Learning, Saurabh Kumar Soni
Master’s Dissertations
In this work I build a system that recognizes isolated American Sign Language (ASL) words, and I use it to ask one fairly direct question: when training data is scarce, is it better to look at the video pixels or at the geometry of the signer’s body? To find out, I train two very different models on exactly the same clips. The first is appearance-based. Every frame is run through standard preprocessing and a ResNet50 backbone pre-trained on ImageNet, which turns it into a 2048-dimensional feature vector, and a Bidirectional LSTM then reads that sequence over time. The second model …
Enhanced Embedding For Multimodal Medical Visual Question And Answering, Akash Suna
Enhanced Embedding For Multimodal Medical Visual Question And Answering, Akash Suna
Master’s Dissertations
Visual Answering of questions in the field of Medical which is called as (VqA) has grown as a dominant area of research that fuse processing of natural language and vision of computer often known as CV or NLP to assist in medical decision-making. However, effective multimodal fusion between medical images and clinical questions remains a significant challenge. This thesis examines the application of the Perceiver IO architecture as an efficient multimodal aggregator for medical VQA. The work has been carried out in multiple directions. First, a classification-based framework is developed by combining Vision Transformer (ViT) and ClinicalBERT alongside a Perceiver …
An Empirical Study Of Rlvr Fine-Tuning For Mathematical Problem Solving In Llms, Rashmi Konnur
An Empirical Study Of Rlvr Fine-Tuning For Mathematical Problem Solving In Llms, Rashmi Konnur
Master’s Dissertations
Large language models have shown immense improvement in coding and math performances thanks to reinforcement learning boosted algorithms. However, its true impact on broadening the reasoning and analytical capacities of an LLM is still contended. In this dissertation, we outline the foundations of Large Language Models, and delve into Reinforcement Learning with Verifiable Rewards (RLVR). We discuss various strategies to efficiently manipulate memory during a fine tuning update. We finally perform RLVR fine-tuning techniques on different models with varied use cases and compare their performances, which corroborate the efficiency of RLVR.
Bridging Cultural Gaps In Enhancing Mental Health Support For The Bhutanese Community, Subash Chandra Majhi
Bridging Cultural Gaps In Enhancing Mental Health Support For The Bhutanese Community, Subash Chandra Majhi
Walden Dissertations and Doctoral Studies
No abstract provided.
Licensed Professional Counselors’ Experiences Of Treating Youth With Autism Spectrum Disorder, Jazzmine Dallas
Licensed Professional Counselors’ Experiences Of Treating Youth With Autism Spectrum Disorder, Jazzmine Dallas
Walden Dissertations and Doctoral Studies
No abstract provided.
Freedom Of Thought And Speech: Charles C. Burleigh And The Refraction Of Quaker Principles In Abolitionist Non-Violence, Jennifer Rycenga
Freedom Of Thought And Speech: Charles C. Burleigh And The Refraction Of Quaker Principles In Abolitionist Non-Violence, Jennifer Rycenga
Faculty Research, Scholarly, and Creative Activity
Charles C. Burleigh (1810-1878), an important American Abolitionist speaker, agent, and theorist, was also an advocate for non-violent resistance. He had many opportunities across his 30+ years fighting slavery to test his non-violent principles, due to mob violence against Abolitionist gatherings. At some of the most significant of these events, such as the destruction of the Abolitionist's Pennsylvania Hall in 1838, Burleigh witnessed the strength and resolve of African-American and white women in upholding non-violence and moral suasion.
While Burleigh never became a member of the Society of Friends, his life and thought run parallel to ideas and practices of …
The Political Culture Of Taxation, Steven M. Karceski, Steven Pfaff
The Political Culture Of Taxation, Steven M. Karceski, Steven Pfaff
Sociology Faculty Articles and Research
In My Tax Dollars: The Morality of Taxpaying in America, Ruth Braunstein explores the political culture of taxation in contemporary America. Calling our attention to the origins of contemporary tax debates, she shows how morality and American civil religion influence how citizens understand taxation, citizenship, and the proper scope of government. In this article, we review Braunstein's main arguments and the evidence she presents in support of them. We take stock of the book's theoretical frame work and the applicability of the concepts it borrows from the sociology of religion. For all there is to like about Braunstein's book, …
Operational Feasibility Of Reinforcement Learning For Vehicle Routing Under Heterogeneous Fleet Capacity Constraints, Freddy Giovanny Aviles Moreno
Operational Feasibility Of Reinforcement Learning For Vehicle Routing Under Heterogeneous Fleet Capacity Constraints, Freddy Giovanny Aviles Moreno
Electronic Theses and Dissertations 2020 - Present
Reinforcement learning methods have demonstrated strong performance on vehicle routing benchmarks, yet their behavior under severe capacity constraints remains unexplored. This dissertation investigates whether PPO-based neural routing policies maintain operational viability when vehicle capacity is severely constrained, as occurs in resource-limited rural logistics settings.
Through controlled experiments on synthetic instances and validation on real-world rural healthcare networks in Florida, this research reveals a critical capacity threshold effect. Moderate capacity reductions from 40 to 20 produce negligible performance loss (4.1%), while severe reductions to capacity 10 trigger catastrophic failure with 243% degradation, manifested through degenerate single-customer routing patterns. Convergence analysis identifies …
A Portable Device To Elicit Sensory Trick And Monitor Forearm Muscle Activity In Pianists With Musician’S Dystonia, Josef Franczak
A Portable Device To Elicit Sensory Trick And Monitor Forearm Muscle Activity In Pianists With Musician’S Dystonia, Josef Franczak
USF Tampa Graduate Theses and Dissertations
Musician’s Dystonia (MD) is a condition that can be acquired in professional musicians,causing involuntary contractions and the loss of fine motor control. The most common pharmaceutical treatment, botulinum toxin injections, has been shown to have side effects of muscle weakness and atrophy. The sensory trick, shown to temporarily relieve symptoms of dystonia, has potential to be used as an alternative or alongside current therapies, allowing patients with MD to return to playing music professionally. To systematically deploy the sensory trick and monitor forearm muscle activity, the first prototype of the Wearable Sensorimotor Integrator (wSMI) has been developed.
The wSMI is …
Neddylation Maintains Oocyte Quality By Stabilizing Mitochondrial Transcription, Avery A Ahmed, Tessa E Steenwinkel, Bethany K Patton, Peixin Jiang, Matthew D Meyer, Jaspreet K Rishi, Mei Leng, Alexander B Saltzman, Elizabeth S Anaya, Momal Sharif, Laurie J Mckenzie, Laura Detti, Anna Malovannaya, Sean M Hartig, Stephanie A Pangas
Neddylation Maintains Oocyte Quality By Stabilizing Mitochondrial Transcription, Avery A Ahmed, Tessa E Steenwinkel, Bethany K Patton, Peixin Jiang, Matthew D Meyer, Jaspreet K Rishi, Mei Leng, Alexander B Saltzman, Elizabeth S Anaya, Momal Sharif, Laurie J Mckenzie, Laura Detti, Anna Malovannaya, Sean M Hartig, Stephanie A Pangas
Faculty, Staff and Students Publications
Age-related decline in oocyte quality increases the risk of infertility, miscarriage, and birth defects. Mitochondrial dysfunction is a key contributor to this decline. Here, we report that oocyte-specific deletion of Uba3, which encodes the catalytic subunit of the E1 NEDDylation-activating complex, causes sterility in mice. Fully grown, germinal vesicle–stage Uba3 conditional knockout oocytes exhibit mitochondrial dysfunction, including elevated reactive oxygen species, impaired oxidative phosphorylation, and depletion of mitochondrially encoded RNA transcripts. Proteomic analysis identified alterations in mitochondrial-associated proteins, including enrichment of mitochondrial matrix and respiratory chain components and reduced abundance of electron transport chain complexes. These defects were associated …
Conformational Cycling Of The Wntless Transporter Drives Trafficking And Secretion Of Wnt Morphogens, Yunhui Ge, Taciani De Almeida Magalhaes, Hongjiang Wu, Dick J H Van Den Boomen, Thu Uyen Nguyen, Tongyi Dou, Gaya P Yadav, Sukyeong Lee, Zhao Wang, Andrew Lemoff, Xuemei Luo, Sumitha S Menon, Min Zhang, Jin Wang, Zhicheng Jin, Jiansen Jiang, Adrian Salic, Pengxiang Huang
Conformational Cycling Of The Wntless Transporter Drives Trafficking And Secretion Of Wnt Morphogens, Yunhui Ge, Taciani De Almeida Magalhaes, Hongjiang Wu, Dick J H Van Den Boomen, Thu Uyen Nguyen, Tongyi Dou, Gaya P Yadav, Sukyeong Lee, Zhao Wang, Andrew Lemoff, Xuemei Luo, Sumitha S Menon, Min Zhang, Jin Wang, Zhicheng Jin, Jiansen Jiang, Adrian Salic, Pengxiang Huang
Faculty, Staff and Students Publications
Wnt proteins are lipid-modified morphogens fundamental in development and disease. During Wnt biogenesis, the G-protein-coupled receptor (GPCR)-like transporter Wntless (WLS) escorts lipidated Wnts from the endoplasmic reticulum to the plasma membrane, then transfers them to extracellular carriers, forming active and soluble morphogen-carrier complexes. To dissect the mechanisms involved, we solve cryo-EM structures of Wnt-bound WLS and unliganded WLS, and perform structure-guided functional experiments. Wnts engage WLS via three conserved hairpins, which are all required for Wnt trafficking to the cell surface and carrier-mediated secretion. Wnt release from cells is driven by dramatic conformational changes in the WLS transmembrane domain, reminiscent …