Digital Commons Network^™

Tension Activation Of Mechanosensitive Two-Pore Domain K+ Channels Traak, Trek-1, And Trek-2., Ben Sorum, Trevor Docter, Vincent Panico, Robert A Rietmeijer, Stephen G Brohawn

Cooper Medical School of Rowan University Departmental Research

TRAAK, TREK-1, and TREK-2 are mechanosensitive two-pore domain K+ (K2P) channels that contribute to action potential propagation, sensory transduction, and muscle contraction. While structural and functional studies have led to models that explain their mechanosensitivity, we lack a quantitative understanding of channel activation by membrane tension. Here, we define the tension response of mechanosensitive K2Ps using patch-clamp recording and imaging. All are low-threshold mechanosensitive channels (T10%/50% 0.6-2.7 / 4.4-6.4 mN/m) with distinct response profiles. TRAAK is most sensitive, TREK-1 intermediate, and TREK-2 least sensitive. TRAAK and TREK-1 are activated broadly over a range encompassing nearly all physiologically relevant …

My Dead Boyfriend Is A Robot (2024), Preston Crowder

Season 2023-2024

No abstract provided.

Flute Studio Recital" "Colors Of Nature", Arkansas State University Department Of Music

Performance Programs

No abstract provided.

Individual Differences In Cannabis Use Disorder With Implications For Endocannabinoid Modulation In Therapeutics Development, Erin L. Martin

MUSC Theses and Dissertations

Cannabis use disorder (CUD) is increasingly prevalent in the United States, but there is no effective pharmacological means to treat it. The endocannabinoid (eCB) system has emerged as a candidate therapeutic target demonstrating some evidence of efficacy in treating CUD. However, clinical trials evaluating eCB-modulating therapeutics have historically undervalued individual differences that could contribute to variation in treatment outcome (e.g. sex, comorbid psychiatric illness). To address this gap in the literature, the present set of studies (a) compared plasma eCB tone in groups underrepresented in treatment trials for CUD (females, individuals with comorbid major depressive disorder; MDD/CUD) with males or …

The Holobiont, Food Justice, And Gaia 2.0 A Post-Human(Ist) Approach To Functional Medicine, Rosalynn A. Vega

Anthropology Faculty Publications

Functional medicine is a personalized and holistic approach to treating chronic disease. In this article, I build upon posthumanist literature by examing how functional medicine practitioners are decentering and destabilizing what it means to be human. Functional medicine discourse on the holobiont, which considers the human as an assemblage of different microbial species, reframes the “humananimal” (see Nayar 2018) as the “humicrobe.” I engage Gaia 2.0 (see Lenton and Latour 2018) when analyzing the interconnectivity, interdependence, and mutualism of all life. My approach to interconnectivity interweaves both functional medicine descriptions of systems biology and Luhmann’s (2012) approach to system’s theory …

The Implementation Of Animal Assisted Therapy In Adult Neurological Rehabilitation, Rachel Camilleri

OTD Capstone Projects

This doctoral capstone project was completed through a partnership with Pi Beta Phi Rehabilitation Institute at Vanderbilt University Medical Center. The focus of this project was to develop animal assisted therapy interventions that could be utilized amongst occupational therapy practitioners with the clinic’s facility dog in an effort to increase utilization of animal assisted therapy. The process for this project required a thorough review of the literature on animal assisted therapy and neurological rehabilitation, conversations with current and past facility dog teams to learn about their work, and trialing animal assisted therapy activities in patient sessions. The outcome of this …

Barcoding Bodies: Rfid Technology And The Perils Of E-Carceration, Jackson Samples

Duke Law & Technology Review

Electronic surveillance now plays a central role in the criminal legal system. Every year, hundreds of thousands of people are tracked by ankle monitors and smartphone technology. And frighteningly, commentators and policymakers have now proposed implanting radio frequency identification (“RFID”) chips into people’s bodies for surveillance purposes. This Note examines the unique risks of these proposals—particularly with respect to people on probation and parole—and argues that RFID implants would constitute a systematic violation of individual privacy and bodily integrity. As a result, they would also violate the Fourth Amendment.

The Comment, April 11, 2024, Bridgewater State University

The Comment

No abstract provided.

Spectator 2024-04-11, Editors Of The Spectator

The Spectator

No abstract provided.

Vol.51 N.38 April 11th 2024, Voice Media Ventures

Black Voice News

No abstract provided.

Enhancing Srna Confidence: Integrating Simulation-Based Crm Training With Didactic Education To Improve Intraoperative Recognition, Communication, And Management Of Intraoperative Cardiac Arrest, Melissa Lee

Doctor of Nursing Practice Scholarly Projects

Within the operating room (OR), the ability to identify deteriorating patient conditions and effectively communicate, is essential to delivering safe and efficient care. Although rare, the critical nature of emergent events necessitates early recognition and prompt intervention by an experienced anesthesia provider. The complexity of emergency management is often related to diagnostic ambiguity, individual provider experience, and disruptions of the fast-paced OR environment, requiring appropriate training methods for proficient execution. However, student registered nurse anesthetists (SRNAs) often lack sufficient exposure to emergent clinical scenarios, leading to delayed intervention and diminished confidence during crisis management. An evidence-based simulation education guideline for …

Kenyon Collegiate - April 11, 2024

Kenyon Collegiate

No abstract provided.

2024 Undergraduate Research Symposium Program, Coastal Carolina University

Undergraduate Research Symposium Programs

15th Annual Undergraduate Research Symposium, April 10-11, 2024.

Engineering Mesenchymal Stromal Cells To Suppress Inflammation And Increase Beta-Cell Mass For Treating Type 1 Diabetes, Dhruva Patel

MUSC Theses and Dissertations

Type 1 diabetes (T1D) is a life-threatening autoimmune disease where the immune system destroys the insulin producing beta-cells in the pancreas. There is no cure for the disease, and the current treatment involves life-long, daily injection of insulin. An ideal therapy for T1D will be the suppression of autoimmunity and increased functional beta-cell mass. The immune regulatory cytokine IL10 can suppress inflammation in various autoimmune diseases. On the other hand, several growth factors and peptide hormones are known to promote an increase in beta-cell mass, through neogenesis and beta-cell proliferation. However, efficient induction of increased beta-cell mass requires preferential and …

The Current - Volume 34 Issue 12, Nova Southeastern University

Student Newspapers

No abstract provided.

Independent- Apr. 11, 2024, Leslie Lozada

The Independent (1988-present)

No abstract provided.

The Effects Of Therapy Dogs On Students, Chloe Romack

Honors Theses

This thesis explores the effects therapy dogs have on students. It begins with a literature review of 17 articles that involve animal-assisted interventions with students and children. The main topics covered include the definition of a therapy animal and how dogs can influence learning, socialization, emotional well-being, anxiety, and depression. It also discusses how dogs can be used in therapy and special education classrooms as well as the major concerns of dogs in schools. Drawing upon the previous research, a survey was sent to school staff in the Paw Paw Public School District. The participants of this study consisted of …

Virtual Gaming, Actual Damage: Video Game Design That Intentionally And Successfully Addicts Users Constitutes Civil Battery, Allison Caffarone

Duke Law & Technology Review

In recent years, there has been increased academic interest in both the neurological effects of compulsive gaming and the potential tort liability of game developers who scientifically engineer games in order to addict users. Scholars from various disciplines are currently debating the scope and potential solutions to the problems associated with Gaming Disorder, now a globally recognized illness. This article contributes to this discussion by offering a multidisciplinary analysis of the scope of video game addiction, its neurological bases, and its relation to the legal rights and responsibilities of victims and game developers. In addition, this article explores the practical …

Protecting American Children: A Social-Ecological Analysis Of Pactp4, Julia Rossmiller

Student Works

The quantity of pesticides used annually in the United States exceeds one billion pounds every year, and the amount is only rising. The current law that governs the use of pesticides—The Federal Insecticide, Fungicide and Rodenticide Act (FIFRA), permits the use of 72 known pesticides that have been proven harmful and are banned in other countries. Research links the usage of pesticides to the health of individuals, families, and whole communities within the environment. Moreover, dangerous pesticides have been shown to disproportionately affect children, agricultural workers, people of color, and low-income communities. Despite the mounting evidence that confirms the adverse …

Identifying A Group Of Factors Predicting Cognitive Impairment Among Older Adults, Longgang Zhao, Yuan Wang, Eric Mishio Bawa, Zichun Meng, Jingkai Wei, Sarah Newman-Norlund, Tushar Trivedi, Hatice Hasturk, Roger Newman-Norlund, Julius Fridriksson Ph.D., Anwar T. Nerchant

Faculty Publications

Background

Cognitive impairment has multiple risk factors spanning several domains, but few studies have evaluated risk factor clusters. We aimed to identify naturally occurring clusters of risk factors of poor cognition among middle-aged and older adults and evaluate associations between measures of cognition and these risk factor clusters.

Methods

We used data from the National Health and Nutrition Examination Survey (NHANES) III (training dataset, n = 4074) and the NHANES 2011–2014 (validation dataset, n = 2510). Risk factors were selected based on the literature. We used both traditional logistic models and support vector machine methods to construct a composite score …

2024 Conference Program, Georgia Southern University

South East Coastal Conference on Languages & Literatures (SECCLL)

2024 Conference Program

The World Health Organization Was Born As A Normative Agency: Seventy-Five Years Of Global Health Law Under Who Governance, Lawrence O. Gostin, Benjamin Mason Meier, Safura Abdool Karim, Judith Bueno De Mesquita, Gian Luca Burci, Danwood Chirwa, Alexandra Finch, Eric A. Friedman, Roojin Habibi, Sam F. Halabi, Tsung-Ling Lee, Brigit Toebes, Pedro Villarreal

Georgetown Law Faculty Publications and Other Works

The World Health Organization (WHO) was born as a normative agency and has looked to global health law to structure collective action to realize global health with justice. Framed by its constitutional authority to act as the directing and coordinating authority on international health, WHO has long been seen as the central actor in the development and implementation of global health law. However, WHO has faced challenges in advancing law to prevent disease and promote health over the past 75 years, with global health law constrained by new health actors, shifting normative frameworks, and soft law diplomacy. These challenges were …

Charting The Cellular Biogeography In Colitis Reveals Fibroblast Trajectories And Coordinated Spatial Remodeling, Paolo Cadinu, Kisha N Sivanathan, Aditya Misra, Rosalind J Xu, Davide Mangani, Evan Yang, Joseph M Rone, Katherine Tooley, Yoon-Chul Kye, Lloyd Bod, Ludwig Geistlinger, Tyrone Lee, Randall T Mertens, Noriaki Ono, Gang Wang, Liliana Sanmarco, Francisco J Quintana, Ana C Anderson, Vijay K Kuchroo, Jeffrey R Moffitt, Roni Nowarski

Faculty, Staff and Student Publications

Gut inflammation involves contributions from immune and non-immune cells, whose interactions are shaped by the spatial organization of the healthy gut and its remodeling during inflammation. The crosstalk between fibroblasts and immune cells is an important axis in this process, but our understanding has been challenged by incomplete cell-type definition and biogeography. To address this challenge, we used multiplexed error-robust fluorescence in situ hybridization (MERFISH) to profile the expression of 940 genes in 1.35 million cells imaged across the onset and recovery from a mouse colitis model. We identified diverse cell populations, charted their spatial organization, and revealed their polarization …

Quaker Campus, April 11, 2024 (Vol. 22, Issue 13), Whittier College

Quaker Campus

No abstract provided.

Bacterial And Viral Co-Infections In Aquaculture Under Climate Warming: Co-Evolutionary Implications, Diagnosis, And Treatment, S Vega-Heredia, I Giffard-Mena, M Reverter

School of Biological and Marine Sciences

Climate change and the associated environmental temperature fluctuations are contributing to increases in the frequency and severity of disease outbreaks in both wild and farmed aquatic species. This has a significant impact on biodiversity and also puts global food production systems, such as aquaculture, at risk. Most infections are the result of complex interactions be - tween multiple pathogens, and understanding these interactions and their co-evolutionary mechanisms is crucial for developing effective diagnosis and control strategies. In this review, we discuss current knowledge on bacteria-bacteria, virus-virus, and bacterial and viral co-infections in aquaculture as well as their co-evolution in the …

Kenyon Collegian - April 11, 2024

The Kenyon Collegian

No abstract provided.

Macromolecular Condensation Organizes Nucleolar Sub-Phases To Set Up A Ph Gradient, Matthew R King, Kiersten M Ruff, Andrew Z Lin, Avnika Pant, Mina Farag, Jared M Lalmansingh, Tingting Wu, Martin J Fossat, Wei Ouyang, Matthew D Lew, Emma Lundberg, Michael D Vahey, Rohit V Pappu

2020-Current year OA Pubs

Nucleoli are multicomponent condensates defined by coexisting sub-phases. We identified distinct intrinsically disordered regions (IDRs), including acidic (D/E) tracts and K-blocks interspersed by E-rich regions, as defining features of nucleolar proteins. We show that the localization preferences of nucleolar proteins are determined by their IDRs and the types of RNA or DNA binding domains they encompass. In vitro reconstitutions and studies in cells showed how condensation, which combines binding and complex coacervation of nucleolar components, contributes to nucleolar organization. D/E tracts of nucleolar proteins contribute to lowering the pH of co-condensates formed with nucleolar RNAs in vitro. In cells, this …

Nardilysin-Regulated Scission Mechanism Activates Polo-Like Kinase 3 To Suppress The Development Of Pancreatic Cancer, Jie Fu, Jianhua Ling, Ching-Fei Li, Chi-Lin Tsai, Wenjuan Yin, Junwei Hou, Ping Chen, Yu Cao, Ya'an Kang, Yichen Sun, Xianghou Xia, Zhou Jiang, Kenei Furukawa, Yu Lu, Min Wu, Qian Huang, Jun Yao, David H Hawke, Bih-Fang Pan, Jun Zhao, Jiaxing Huang, Huamin Wang, E I Mustapha Bahassi, Peter J Stambrook, Peng Huang, Jason B Fleming, Anirban Maitra, John A Tainer, Mien-Chie Hung, Chunru Lin, Paul J Chiao

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) develops through step-wise genetic and molecular alterations including Kras mutation and inactivation of various apoptotic pathways. Here, we find that development of apoptotic resistance and metastasis of KrasG12D-driven PDAC in mice is accelerated by deleting Plk3, explaining the often-reduced Plk3 expression in human PDAC. Importantly, a 41-kDa Plk3 (p41Plk3) that contains the entire kinase domain at the N-terminus (1-353 aa) is activated by scission of the precursor p72Plk3 at Arg354 by metalloendopeptidase nardilysin (NRDC), and the resulting p32Plk3 C-terminal Polo-box domain (PBD) is removed by proteasome degradation, preventing the inhibition of p41Plk3 by PBD. We find …

Leveraging 4-H To Address Emergent Health Needs During The Covid-19 Pandemic: Epidemiology Pilot Project, Anne Marie Iaccopucci, Marcel Horowitz, Dorina Espinoza, Roshan Nayak

Journal of Extension

4-H academics responded to the Covid pandemic by rapidly adapting CDC and other resources for virtual delivery. A statewide epidemiology project was taught to 48 youth with the goal of minimizing fears and confusion, increasing prevention measures, leveraging current topics for education, and bolstering the social-emotional health of youth participants. Results indicated that youth enjoyed the project and adopted behaviors to reduce the spread of infectious diseases, such as increased mask-wearing.

Chemotherapy Activates Inflammasomes To Cause Inflammation-Associated Bone Loss, Chun Wang, Khushpreet Kaur, Canxin Xu, Yousef Abu-Amer, Gabriel Mbalaviele

2020-Current year OA Pubs

Chemotherapy is a widely used treatment for a variety of solid and hematological malignancies. Despite its success in improving the survival rate of cancer patients, chemotherapy causes significant toxicity to multiple organs, including the skeleton, but the underlying mechanisms have yet to be elucidated. Using tumor-free mouse models, which are commonly used to assess direct off-target effects of anti-neoplastic therapies, we found that doxorubicin caused massive bone loss in wild-type mice, a phenotype associated with increased number of osteoclasts, leukopenia, elevated serum levels of danger-associated molecular patterns (DAMPs; e.g. cell-free DNA and ATP) and cytokines (e.g. IL-1β and IL-18). Accordingly, …