Digital Commons Network^™

Genome-Wide Screening Identifies Trim33 As An Essential Regulator Of Dendritic Cell Differentiation, Ioanna Tiniakou, Pei-Feng Hsu, Lorena S Lopez-Zepeda, Görkem Garipler, Eduardo Esteva, Nicholas M Adams, Geunhyo Jang, Chetna Soni, Colleen M Lau, Fan Liu, Alireza Khodadadi-Jamayran, Tori C Rodrick, Drew Jones, Aristotelis Tsirigos, Uwe Ohler, Mark T Bedford, Stephen D Nimer, Vesa Kaartinen, Esteban O Mazzoni, Boris Reizis

Faculty, Staff and Student Publications

The development of dendritic cells (DCs), including antigen-presenting conventional DCs (cDCs) and cytokine-producing plasmacytoid DCs (pDCs), is controlled by the growth factor Flt3 ligand (Flt3L) and its receptor Flt3. We genetically dissected Flt3L-driven DC differentiation using CRISPR-Cas9-based screening. Genome-wide screening identified multiple regulators of DC differentiation including subunits of TSC and GATOR1 complexes, which restricted progenitor growth but enabled DC differentiation by inhibiting mTOR signaling. An orthogonal screen identified the transcriptional repressor Trim33 (TIF-1γ) as a regulator of DC differentiation. Conditional targeting in vivo revealed an essential role of Trim33 in the development of all DCs, but not of monocytes …

Non-Traumatic Osteonecrosis Of The Femoral Head Induced By Steroid And Alcohol Exposure Is Associated With Intestinal Flora Alterations And Metabolomic Profiles, Qing-Yuan Zheng, Ye Tao, Lei Geng, Peng Ren, Ming Ni, Guo-Qiang Zhang

Faculty, Staff and Student Publications

OBJECTIVE: Osteonecrosis of the femoral head (ONFH) is a severe disease that primarily affects the middle-aged population, imposing a significant economic and social burden. Recent research has linked the progression of non-traumatic osteonecrosis of the femoral head (NONFH) to the composition of the gut microbiota. Steroids and alcohol are considered major contributing factors. However, the relationship between NONFH caused by two etiologies and the microbiota remains unclear. In this study, we examined the gut microbiota and fecal metabolic phenotypes of two groups of patients, and analyzed potential differences in the pathogenic mechanisms from both the microbial and metabolic perspectives.

METHODS: …

In Silico And In Vitro Study Of Isoquercitrin Against Kidney Cancer And Inflammation By Triggering Potential Gene Targets, Safia Iqbal, Md Rezaul Karim, Shahnawaz Mohammad, Jong Chan Ahn, Anjali Kariyarath Valappil, Ramya Mathiyalagan, Deok-Chun Yang, Dae-Hyo Jung, Hyocheol Bae, Dong Uk Yang

Faculty, Staff and Student Publications

Kidney cancer has emerged as a major medical problem in recent times. Multiple compounds are used to treat kidney cancer by triggering cancer-causing gene targets. For instance, isoquercitrin (quercetin-3-O-β-d-glucopyranoside) is frequently present in fruits, vegetables, medicinal herbs, and foods and drinks made from plants. Our previous study predicted using protein-protein interaction (PPI) and molecular docking analysis that the isoquercitrin compound can control kidney cancer and inflammation by triggering potential gene targets of IGF1R, PIK3CA, IL6, and PTGS2. So, the present study is about further in silico and in vitro validation. We performed molecular dynamic (MD) simulation, gene ontology (GO), Kyoto …

Coronary Microvascular Dysfunction In Childhood: An Emerging Pathological Entity And Its Clinical Implications, Takeshi Tsuda, Gina Patel

Department of Pediatrics Faculty Papers

Coronary microvascular dysfunction (CMD) encompasses a spectrum of structural and functional alterations in coronary microvasculature resulting in impaired coronary blood flow and consequent myocardial ischemia without obstruction in epicardial coronary artery. The pathogenesis of CMD is complex involving both functional and structural alteration in the coronary microcirculation. In adults, CMD is predominantly discussed in context with anginal chest pain or existing ischemic heart disease and its risk factors. The presence of CMD suggests increased risk of adverse cardiovascular events independent of coronary atherosclerosis. Coronary microvascular dysfunction is also known in children but is rarely recognized due to paucity of concommitent …

Diverse Habitats Shape The Movement Ecology Of A Top Marine Predator, The White Shark Carcharodon Carcharias, Oliver J. D. Jewell, Taylor K. Chapple, Salvador J. Jorgensen, Paul Kanive, Jerry H. Moxley, James R. Tweedley, Scot Anderson, Barbara A. Block, Adrian C. Gleiss

Marine Science Faculty Publications and Presentations

An animal's movement is influenced by a plethora of internal and external factors, leading to individual- and habitat-specific movement characteristics. This plasticity is thought to allow individuals to exploit diverse environments efficiently. We tested whether the movement characteristics of white sharks Carcharodon carcharias differ across ontogeny and among habitats along the coast of Central California. In doing so, we elucidate how changes in internal state (physiological changes coinciding with body size) and external environments (differing seascapes and/or diel phases) shape the movement of this globally distributed predator. Twenty-one white sharks, from small juveniles to large adults, were equipped with motion-sensitive …

Shots Fired, Shots Refused: Scientific, Ethical & Legal Challenges Surrounding The U.S. Military's Covid-19 Vaccine Mandate, Shawn Mckelvy, L. William Uhl, Armand Balboni

St. Mary's Law Journal

The COVID-19 pandemic provided uncertain and challenging circumstances under which to lead a nation and the military that protects it. Those in charge and in command faced unique challenges—scientific, ethical, and legal—at our various levels of government to both keep people safe while keeping government and society functioning. While there were many successes to celebrate, there are also many criticisms for how this “whole-of-government approach” may have degraded some of our most cherished liberties along the way. The authors focus on the U.S. military’s vaccine mandate and propose military leaders may have failed to fully consider the evolving science, weigh …

Phase 1b Study Of Intraperitoneal Ipilimumab And Nivolumab In Patients With Recurrent Gynecologic Malignancies With Peritoneal Carcinomatosis, Anne Knisely, Emily Hinchcliff, Bryan Fellman, Ann Mosley, Kathryn Lito, Sara Hull, Shannon N Westin, Anil K Sood, Kathleen M Schmeler, Jolyn S Taylor, Steven Y Huang, Rahul A Sheth, Karen H Lu, Amir A Jazaeri

Faculty, Staff and Student Publications

Background: Intravenous immune checkpoint blockade (ICB) has shown poor response rates in recurrent gynecologic malignancies. Intraperitoneal (i.p.) ICB may result in enhanced T cell activation and anti-tumor immunity.

Methods: In this phase 1b study, registered at Clinical.

Trials: gov (NCT03508570), initial cohorts received i.p. nivolumab monotherapy, and subsequent cohorts received combination i.p. nivolumab every 2 weeks and i.p. ipilimumab every 6 weeks, guided by a Bayesian design. The primary objective was determination of the recommended phase 2 dose (RP2D) of the combination. Secondary outcomes included toxicity, objective response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Findings: …

Combined Deletion Of Men1, Atrx And Pten Triggers Development Of High-Grade Pancreatic Neuroendocrine Tumors In Mice, Mary Esmeralda Fuentes, Xiaoyin Lu, Natasha M Flores, Simone Hausmann, Pawel K Mazur

Faculty, Staff and Student Publications

Pancreatic neuroendocrine tumors (PanNETs) are a heterogeneous group of tumors that exhibit an unpredictable and broad spectrum of clinical presentations and biological aggressiveness. Surgical resection is still the only curative therapeutic option for localized PanNET, but the majority of patients are diagnosed at an advanced and metastatic stage with limited therapeutic options. Key factors limiting the development of new therapeutics are the extensive heterogeneity of PanNETs and the lack of appropriate clinically relevant models. In that context, genomic sequencing of human PanNETs revealed recurrent mutations and structural alterations in several tumor suppressors. Here, we demonstrated that combined loss of MEN1, …

The George-Anne Daily Deep Dive, Georgia Southern University

George-Anne Media Group: Newsletters & Magazines

No abstract provided.

Comics About Organs: Social Support And Graphic Medicine In The Awkward Yeti, Jacob Gibb

Theses and Dissertations

Comic strips have always been a medium for more than humor. Many comic strips published on social media sites address mental health issues. Despite their popularity, the field of graphic medicine, which examines the intersection of health care and comics, has seen little to no examination of how these comics function on social media. Utilizing the popular comic The Awkward Yeti, this research examines how the rhetorical devices of comics make them an ideal medium for communicating about mental health issues and providing social support to social media users. This study conducts a content analysis of 15 Instagram posts from …

Serum Neuroactive Metabolites Of The Tryptophan Pathway In Patients With Acute Phase Of Affective Disorders, Yanli Li, Leilei Wang, Junchao Huang, Ping Zhang, Yanfang Zhou, Jinghui Tong, Wenjin Chen, Mengzhuang Gou, Baopeng Tian, Wei Li, Xingguang Luo, Li Tian, L Elliot Hong, Chiang-Shan R Li, Yunlong Tan

Faculty, Staff and Student Publications

BACKGROUND: Many studies showed disrupted tryptophan metabolism in patients with affective disorders. The aims of this study were to explore the differences in the metabolites of tryptophan pathway (TP) and the relationships between TP metabolites and clinical symptoms, therapeutic effect in patients with bipolar disorder with acute manic episode (BD-M), depressive episode (BD-D) and major depressive disorder (MDD).

METHODS: Patients with BD-M (n=52) and BD-D (n=39), MDD (n=48) and healthy controls (HCs, n=49) were enrolled. The serum neuroactive metabolites levels of the TP were measured by liquid chromatography-tandem mass spectrometry. Hamilton Depression Scale-17 item (HAMD-17) and Young Mania Rating Scale …

Storylines Of Family Medicine Xii: Family Medicine And The Healthcare System, Kurt C. Stange

Faculty Scholarship

Storylines of Family Medicine is a 12-part series of thematically linked mini-essays with accompanying illustrations that explore the many dimensions of family medicine, as interpreted by individual family physicians and medical educators in the USA and elsewhere around the world. In 'XII: Family medicine and the future of the healthcare system', authors address the following themes: 'Leadership in family medicine', 'Becoming an academic family physician', 'Advocare-our call to act', 'The paradox of primary care and three simple rules', 'The quadruple aim-melding the patient and the health system', 'Fit-for-purpose medical workforce', 'Universal healthcare-coverage for all', 'The futures of family medicine' and …

2024-04-12 Institutional Animal Care And Use Committee Meeting Minutes, Morehead State University. Faculty Senate., Institutional Animal Care And Use Committee.

Faculty Senate Records

The meeting minutes of the Institutional Animal Care and Use Committee for April 12, 2024.

Miniature Battery-Free Epidural Cortical Stimulators, Joshua E Woods, Amanda L Singer, Fatima Alrashdan, Wendy Tan, Chunfeng Tan, Sunil A Sheth, Sameer A Sheth, Jacob T Robinson

Faculty, Staff and Student Publications

Miniaturized neuromodulation systems could improve the safety and reduce the invasiveness of bioelectronic neuromodulation. However, as implantable bioelectronic devices are made smaller, it becomes difficult to store enough power for long-term operation in batteries. Here, we present a battery-free epidural cortical stimulator that is only 9 millimeters in width yet can safely receive enough wireless power using magnetoelectric antennas to deliver 14.5-volt stimulation bursts, which enables it to stimulate cortical activity on-demand through the dura. The device has digitally programmable stimulation output and centimeter-scale alignment tolerances when powered by an external transmitter. We demonstrate that this device has enough power …

Akap12 Upregulation Associates With Pde8a To Accelerate Cardiac Dysfunction, Hanan Qasim, Mehrdad Rajaei, Ying Xu, Arfaxad Reyes-Alcaraz, Hala Y Abdelnasser, M David Stewart, Satadru K Lahiri, Xander H T Wehrens, Bradley K Mcconnell

Faculty, Staff and Students Publications

BACKGROUND: In heart failure, signaling downstream the β2-adrenergic receptor is critical. Sympathetic stimulation of β2-adrenergic receptor alters cAMP (cyclic adenosine 3',5'-monophosphate) and triggers PKA (protein kinase A)-dependent phosphorylation of proteins that regulate cardiac function. cAMP levels are regulated in part by PDEs (phosphodiesterases). Several AKAPs (A kinase anchoring proteins) regulate cardiac function and are proposed as targets for precise pharmacology. AKAP12 is expressed in the heart and has been reported to directly bind β2-adrenergic receptor, PKA, and PDE4D. However, its roles in cardiac function are unclear.

METHODS: cAMP accumulation in real time downstream of the β2-adrenergic receptor was detected for …

Influence Of Rna Circularity On Target Rna-Directed Microrna Degradation, Federico Fuchs Wightman, Jerónimo Lukin, Sebastián A Giusti, Michael Soutschek, Laureano Bragado, Berta Pozzi, María L Pierelli, Paula González, Juan P Fededa, Gerhard Schratt, Rina Fujiwara, Jeremy E Wilusz, Damián Refojo, Manuel De La Mata

Faculty, Staff and Students Publications

A subset of circular RNAs (circRNAs) and linear RNAs have been proposed to 'sponge' or block microRNA activity. Additionally, certain RNAs induce microRNA destruction through the process of Target RNA-Directed MicroRNA Degradation (TDMD), but whether both linear and circular transcripts are equivalent in driving TDMD is unknown. Here, we studied whether circular/linear topology of endogenous and artificial RNA targets affects TDMD. Consistent with previous knowledge that Cdr1as (ciRS-7) circular RNA protects miR-7 from Cyrano-mediated TDMD, we demonstrate that depletion of Cdr1as reduces miR-7 abundance. In contrast, overexpression of an artificial linear version of Cdr1as drives miR-7 degradation. Using plasmids that …

Storylines Of Family Medicine Xii: Family Medicine And The Healthcare System, William B Ventres, Leslie A Stone, Jeannette E South-Paul, Kendall M Campbell, Aerial R Petty, Hima Ekanadham, Kurt C Stange, Rebecca S Etz, William L Miller, Robert L Ferrer, Marianna Kong, Thomas Bodenheimer, Roger Strasser, Sharon C M Reece, Joshua Freeman, John M Westfall

Faculty, Staff and Students Publications

Storylines of Family Medicine is a 12-part series of thematically linked mini-essays with accompanying illustrations that explore the many dimensions of family medicine, as interpreted by individual family physicians and medical educators in the USA and elsewhere around the world. In ‘XII: Family medicine and the future of the healthcare system’, authors address the following themes: ‘Leadership in family medicine’, ‘Becoming an academic family physician’, ‘Advocare—our call to act’, ‘The paradox of primary care and three simple rules’, ‘The quadruple aim—melding the patient and the health system’, ‘Fit-for-purpose medical workforce’, ‘Universal healthcare—coverage for all’, ‘The futures of family …

Human-Machine Communication: Complete Volume. Volume 7 Special Issue: Mediatization

Human-Machine Communication

This is the complete volume of HMC Volume 7. Special Issue on Mediatization

Artificial Sociality, Simone Natale, Iliana Depounti

Human-Machine Communication

This article proposes the notion of Artificial Sociality to describe communicative AI technologies that create the impression of social behavior. Existing tools that activate Artificial Sociality include, among others, Large Language Models (LLMs) such as ChatGPT, voice assistants, virtual influencers, socialbots and companion chatbots such as Replika. The article highlights three key issues that are likely to shape present and future debates about these technologies, as well as design practices and regulation efforts: the modelling of human sociality that foregrounds it, the problem of deception and the issue of control from the part of the users. Ethical, social and cultural …

A New Family Member Or Just Another Digital Interface? Smart Speakers In The Lives Of Families With Young Children, Giovanna Mascheroni

Human-Machine Communication

Based on longitudinal qualitative research involving twenty families with at least one child aged eight or younger, the article provides an account of how families, as distinctive communicative figurations, adopt, use and make sense of smart speakers through diverse socially situated practices. Findings show that parents and children enter in a communicative relationship with smart speakers based on their attribution of human-like or machine-like traits to the device, and the device response to their expectations. Moreover, engaging in communicative practices through and with smart speakers, family members subvert or reinforce existing power relations. However, smart speakers acquire new agency by …

Mediatization And Human-Machine Communication: Trajectories, Discussions, Perspectives, Andreas Hepp, Göran Bolin, Andrea L. Guzman, Wiebke Loosen

Human-Machine Communication

As research fields, mediatization and Human-Machine Communication (HMC) have distinct historical trajectories. While mediatization research is concerned with the fundamental interrelation between the transformation of media and communications and cultural and societal changes, the much younger field of HMC delves into human meaning-making in interactions with machines. However, the recent wave of “deep mediatization,” characterized by an increasing emphasis on general communicative automation and the rise of communicative AI, highlights a shared interest in technology’s role within human interaction. This introductory article examines the trajectories of both fields, demonstrating how mediatization research “zooms out” from overarching questions of societal and …

Smoothing Out Smart Tech’S Rough Edges: Imperfect Automation And The Human Fix, Christian Katzenbach, Christian Pentzold, Paloma Viejo Otero

Human-Machine Communication

In this article, we take issue with an idea of autonomous and efficient automation that is upheld through the paradoxical conjunction of a flawed vision of the technological fix and the under-acknowledged human work required to fill in the gaps between machines and users. Our argument is based on two case studies that sit at opposite tails of automation processes: the front end of self-service checkouts and the back end of content moderation. This juxtaposition allows us to surface three themes on how the hype around automation is enabled by human interventions: the ad-hoc sociality in situated practices of automation, …

Quacking For Noggin: Farm Animal Assisted Therapy For The Traumatic Brain Injury Survivors, Alex Sargsyan

Epsilon Sigma at-Large Research Conference

The Aim

The aim of this study was to examine the effects of Animal Assisted Therapies with Farm Animals (AATF) with domesticated ducks on depression, anxiety, and self-efficacy in patients with traumatic brain injury (TBI) . Furthermore, the following hypothesis was tested:

Engaging in AATF with domesticated ducks will be associated with decrease in depression, anxiety, and an increase in self-efficacy.

Methods

The study examined the effects of AATF on anxiety and depression among patients with TBI. Time series quasi-experimental design structure was utilized. At the beginning of the study participants received Hospital Anxiety and Depression Survey (HADS) and General …

The Last Moon: Delving Into Philippine Culture In A Disney Perspective, Thea Angelee Davis

Celebration of Research

Disney’s cultural movies have undeniably impacted the world, with films like Moana and Encanto introducing specific cultures to the global audience and receiving widespread recognition. Inspired by this influential way of cultural storytelling and noticing the lack of animated movies that represent my native country, the Philippines, I created The Last Moon as a project in ENG250LC. The creation process involved extensive researching into Philippine legends and developing those in a story and storyboard scenes. Ultimately, I designed all the visual graphics that resemble Disney’s iconic style. By integrating elements of the Philippines into a familiar Disney storytelling framework, I …

Metabolic Characterization Of Porcine Hepatocytes, Sophie Swart, Ainslie Robinson, Daiki Jonouchi, Marcus Blankespoor, Kip Cullinan, Zachary Varpness, Ryan Schulze

Celebration of Research

No abstract provided.

The Gut Microbiome In Chronic Diseases, Emily Mcdonald, Katlyn Bombei, Madelyn Weber

Celebration of Research

Cardiovascular disease, chronic kidney disease, diabetes, and various mental health conditions have been shown to be associated with gut microbiota alterations that may contribute to the development and pathogenesis of those diseases. Cardiovascular disease, inflammatory bowel disease and diabetes have been associated with a lack of butyrate- producing bacteria which are responsible for lowering blood pressure, limiting atherosclerosis, abdominal pain, and lowering insulin resistance. Medications for individuals with schizophrenia may decrease bacteria with anti-inflammatory properties. Potential research avenues may be directed at investigating the di@erences between gut microbiomes between men and women to cultivate unique and targeted treatment and prevention …

Animal-Assisted Services And Occupational Therapy Within An Australian Context: A Survey Study, Jessica Hill, Carlie Driscoll, Kelly Pearse

People and Animals: The International Journal of Research and Practice

Introduction: The incorporation of animal-assisted services is an emerging field of occupational therapy. There remains varying levels of involvement and knowledge of what is involved in animal-assisted services among Australian occupational therapists. The profession of occupational therapy acknowledges that competence as a clinician requires appropriate education and knowledge guiding clinical decision making.

Research Question: This study aimed to explore the understanding and role of Australian occupational therapists including animal-assisted services in their practice, as well as the changes required to support future service delivery.

Methods: A cross sectional survey was conducted online. Participants were recruited through a digital mailing list, …

Nutrient Intake, Performance, And Body Composition Of Preseason Wrestlers, Gregory-James Coapstick, Allison Barry, Crystal Levesque, Marni Shoemaker

International Journal of Exercise Science

International Journal of Exercise Science 17(2): 517-530, 2024. College athletes, especially in weight class sports, often experience energy deficits. Athletes competing in weight class sports such as wrestling are at greatest risk for deficiencies and little is known about the relationships between body composition, nutrient intake, and performance in these athletes. The purposes of this study were to (1) quantify macronutrient and micronutrient intake of pre-season male collegiate wrestlers and compare to estimated needs, and (2) examine relationships among nutritional intakes, body composition, and performance measurements of strength and anaerobic capacity. Male Division I wrestlers (n = 11, age: …

The Myopia Epidemic: Exploring The Pathology And Management Of The World’S Most Common Eye Disorder, Gabriella Baldassarre, Bs

inSIGHT

Myopia is the most common eye disorder in the world and an increasing cause of vision impairment in children.¹ Myopia is an imbalance in the refractive components of the eye and its length, leading to inaccurate focusing of light rays in front of the retina tissue instead of on it. There is a spectrum of severity of myopia, from mild nearsightedness to a more severe form known as high or degenerative myopia, which has the potential to cause vision loss and blindness. There is evidence of a developing myopia epidemic, with an estimated one-third of the world population affected …

Insight, Volume 4, Issue 1, 2024

inSIGHT

Contents

8 - The Myopia Epidemic: Exploring the Pathology and Management of the World’s Most Common Eye Disorder
By Gabriella Baldassare

15 - Evolution of Lamellar Keratoplasty
By Ishan Kasat

19 - Bridging the Global Gap of Blindness Through Artificial Intelligence
By Nathan Delacth

24 - CMV Retinitis: An Expert’s Perspective
By Caitlyn Kwun

29 - Macular Holes: Diagnosis, Treatment, and Complications
By Ayra Khan

36 - Chronic Progressive External Ophthalmoplegia
By Bahram Pashaee

39 - Medical Missions, Ethical Considerations, and the Future for Healthcare Delivery in Ophthalmology
By Robert Medina

43 - Shaping the Future of Vision: The Rise …