Digital Commons Network^™

Role Of Ethanolamine Utilization And Bacterial Microcompartment Formation In Listeria Monocytogenes Intracellular Infection, Ayan Chatterjee, Karan Gautam Kaval, Danielle A Garsin

Faculty, Staff and Student Publications

Ethanolamine (EA) affects the colonization and pathogenicity of certain human bacterial pathogens in the gastrointestinal tract. However, EA can also affect the intracellular survival and replication of host cell invasive bacteria such as Listeria monocytogenes (LMO) and Salmonella enterica serovar Typhimurium (S. Typhimurium). The EA utilization (eut) genes can be categorized as regulatory, enzymatic, or structural, and previous work in LMO showed that loss of genes encoding functions for the enzymatic breakdown of EA inhibited LMO intracellular replication. In this work, we sought to further characterize the role of EA utilization during LMO infection of host …

Navigating Diagnostic And Treatment Challenges Of Pulmonary Hypertension In Infants With Bronchopulmonary Dysplasia., Nidhy P. Varghese, Gabriel Altit, Megan M. Gubichuk, Roopa Siddaiah

Manuscripts, Articles, Book Chapters and Other Papers

Advances in perinatal intensive care have significantly enhanced the survival rates of extremely low gestation-al-age neonates but with continued high rates of bronchopulmonary dysplasia (BPD). Nevertheless, as the survival of these infants improves, there is a growing awareness of associated abnormalities in pulmonary vascular development and hemodynamics within the pulmonary circulation. Premature infants, now born as early as 22 weeks, face heightened risks of adverse development in both pulmonary arterial and venous systems. This risk is compounded by parenchymal and airway abnormalities, as well as factors such as inflammation, fibrosis, and adverse growth trajectory. The presence of pulmonary hypertension in …

Hemojuvelin-Mediated Hepcidin Induction Requires Both Bone Morphogenetic Protein Type I Receptors Alk2 And Alk3, Deniz Y Dogan, Eugen I Urzica, Isabelle Hornung, Philipp Kastl, David Oguama, Franca M Fette, Lien H Nguyen, Frank Rosenbauer, Kai Zacharowski, Ursula Klingmüller, Elise Gradhand, Andreas Von Knethen, Rüdiger Popp, Ingrid Fleming, Lisa Schrader, Andrea U Steinbicker

Faculty, Staff and Student Publications

Hemojuvelin (HJV) is a glycosylphosphatidylinositol-anchored protein of the repulsive guidance molecule family acting as a bone morphogenetic protein (BMP) coreceptor to induce the hepatic iron regulatory protein hepcidin. Hepcidin causes ubiquitination and degradation of the sole known iron exporter ferroportin, thereby limiting iron availability. The detailed signaling mechanism of HJV in vivo has yet to be investigated. In the current manuscript, we used an established model of adeno-associated virus (AAV)-mediated liver-specific overexpression of HJV in murine models of hepatocyte-specific deficiency of the BMP type I receptors Alk2 or Alk3. In control mice, HJV overexpression increased hepatic Hamp messenger RNA (mRNA) …

An Inducible Genetic Tool To Track And Manipulate Specific Microglial States Reveals Their Plasticity And Roles In Remyelination, Kia M Barclay, Nora Abduljawad, Min Woo Kim, Lu Zhou, Jin Yang, Justin Rustenhoven, Jose A Mazzitelli, Leon C D Smyth, Dvita Kapadia, Simone Brioschi, Wandy Beatty, Jinchao Hou, Naresha Saligrama, Marco Colonna, Jonathan Kipnis, Qingyun Li, Et Al.

2020-Current year OA Pubs

Recent single-cell RNA sequencing studies have revealed distinct microglial states in development and disease. These include proliferative-region-associated microglia (PAMs) in developing white matter and disease-associated microglia (DAMs) prevalent in various neurodegenerative conditions. PAMs and DAMs share a similar core gene signature. However, the extent of the dynamism and plasticity of these microglial states, as well as their functional significance, remains elusive, partly due to the lack of specific tools. Here, we generated an inducible Cre driver line, Clec7a-CreER

Limited Nerve Regeneration Across Acellular Nerve Allografts (Anas) Coincides With Changes In Blood Vessel Morphology And The Development Of A Pro-Inflammatory Microenvironment, Jesús A Acevedo Cintrón, Daniel A Hunter, Lauren Schellhardt, Deng Pan, Susan E Mackinnon, Matthew D Wood

2020-Current year OA Pubs

The use of acellular nerve allografts (ANAs) to reconstruct long nerve gaps (>3 cm) is associated with limited axon regeneration. To understand why ANA length might limit regeneration, we focused on identifying differences in the regenerative and vascular microenvironment that develop within ANAs based on their length. A rat sciatic nerve gap model was repaired with either short (2 cm) or long (4 cm) ANAs, and histomorphometry was used to measure myelinated axon regeneration and blood vessel morphology at various timepoints (2-, 4- and 8-weeks). Both groups demonstrated robust axonal regeneration within the proximal graft region, which continued across …

Singer V. Bezark

All Decisions

The rent stabilized tenant of record ("landlord" for the purposes of record structure on this project website) initiated a holdover proceeding against their subtenants, seeking to recover possession of the subject premises. The subtenants interposed an answer with affirmative defenses and counterclaims. The court denied the subtenants' motion for discovery and denied the landlord's motion for summary judgment. The court found that the subtenants failed to plead a valid illusory tenancy affirmative defense and that there were disputed issues of fact requiring trial regarding whether a month-to-month tenancy was created after expiration of the sublease agreement. The court also noted …

Frontotemporal Dementia-Like Disease Progression Elicited By Seeded Aggregation And Spread Of Fus, Sonia Vazquez-Sanchez, Britt Tilkin, Fatima Gasset-Rosa, Sitao Zhang, Diana Piol, Melissa Mcalonis-Downes, Jonathan Artates, Noe Govea-Perez, Yana Verresen, Lin Guo, Don Cleveland, James Shorter, Sandrine Da Cruz

Department of Biochemistry and Molecular Biology Faculty Papers

RNA binding proteins have emerged as central players in the mechanisms of many neurodegenerative diseases. In particular, a proteinopathy of fused in sarcoma (FUS) is present in some instances of familial Amyotrophic lateral sclerosis (ALS) and about 10% of sporadic Frontotemporal lobar degeneration (FTLD). Here we establish that focal injection of sonicated human FUS fibrils into brains of mice in which ALS-linked mutant or wild-type human FUS replaces endogenous mouse FUS is sufficient to induce focal cytoplasmic mislocalization and aggregation of mutant and wild-type FUS which with time spreads to distal regions of the brain. Human FUS fibril-induced FUS aggregation …

Tenovin-1, A Selective Sirt1/2 Inhibitor, Attenuates High-Fat Diet-Induced Hepatic Fibrosis Via Inhibition Of Hsc Activation In Zdf Rats, Swati Sharma, Sreevarsha Gali, Amit Kundu, Jae Hyeon Park, Jae-Sung Kim, Hyung Sik Kim

2020-Current year OA Pubs

Type 2 diabetes mellitus (T2DM) increases the risk of non-alcoholic fatty liver disease (NAFLD) progression to advanced stages, especially upon high-fat diet (HFD). HFD-induced hepatic fibrosis can be marked by oxidative stress, inflammation, and activation of hepatic stellate cells. Sirtuin 1/2 (SIRT1/2), NAD-dependent class III histone deacetylases, are involved in attenuation of fibrosis. In our conducted research, TGF-β1-activated LX-2 cells, free fatty acid (FFA)-treated simultaneous co-culture (SCC) cells, and HFD-induced hepatic fibrosis in Zucker diabetic fatty (ZDF) rats, a widely used animal model in the study of metabolic syndromes, were used to evaluate the protective effect of Tenovin-1, a SIRT1/2 …

Rigor And Reproducibility In Human Brain Organoid Research: Where We Are And Where We Need To Go, Soraya O Sandoval, Gerarda Cappuccio, Karina Kruth, Sivan Osenberg, Saleh M Khalil, Natasha M Méndez-Albelo, Krishnan Padmanabhan, Daifeng Wang, Mark J Niciu, Anita Bhattacharyya, Jason L Stein, André M M Sousa, Elisa A Waxman, Elizabeth D Buttermore, Dosh Whye, Carissa L Sirois, Cross-Iddrc Human Stem Cell Consortium, Aislinn Williams, Mirjana Maletic-Savatic, Xinyu Zhao

Faculty, Staff and Students Publications

Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.

Effects Of Ibuprofen In The Zdf Rat Model Of Type 2 Diabetes, Alexander Gosslau, Unsal Ozdogru, Emmanuel Zachariah, Shiming Li, Chi-Tang Ho

Journal of Food and Drug Analysis

We aimed to investigate the therapeutic potential of ibuprofen against type 2 diabetes (T2D) using obese Zucker diabetic fatty (ZDF) rats as type 2 diabetes model. ZDF rats were hyperglycemic, dyslipidemic and expressed pro-inflammatory markers in contrast to lean controls, thus reflecting the relationship between obesity and chronic inflammation promoting T2D. Chronic treatment with ibuprofen (2-(4-Isobutylphenyl)propanoic acid) was used to study the impact on pathological T2D conditions as compared to metformin (1,1-dimethylbiguanide) treated ZDF as well as lean controls. Ibuprofen decreased A1c but induced a high insulin release with improved glucose tolerance only after early time points (i.g., 15 and …

Citrus Depressa Peel Extract Act As A Prebiotic To Reduce Lipid Accumulation And Modulate Gut Microbiota In Obese Mice, Zheng-Yuan Su, Jie-An Liao, Ching-Ting Lin, Guor-Jien Wei, Yen-Chen Tung

Journal of Food and Drug Analysis

Citrus peels contain abundant polyphenols, particularly flavonoids, and have been shown to exert lipid accumulation decreasing ability. In this study, Citrus depressa peel applied to oven drying and extracted with ethanol extract as CDEE to analyze its flavonoids compositions and investigated its effects on a high-fat diet (HFD)-induced obese mice model. CDEE contained several flavonoids such as hesperidin, sinesentin, nobiletin, tangeretin, 5-demethylnobiletin, and 5-demethyltangeretin. The mice fed an HFD, and administration of 2% CDEE to could decrease weight gain, abdominal fat weight, inguinal fat weight, and the adipocyte size, and CDEE also reduced serum total cholesterol (TCHO), triacylglycerol (TG) compared …

Detection Of Aflatoxin B1 In Chili Powder Using Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy, Thin Thin Sein, Urairat Mongmonsin, Patutong Chatchawal, Molin Wongwattanakul, Oranee Srichaiyapol, Rungrueang Pattanakul, Patcharaporn Tippayawat

Journal of Food and Drug Analysis

Aflatoxin B₁, a major global food safety concern, is produced by toxigenic fungi during crop growing, drying, and storage, and shows increasing annual prevalence. This study aimed to detect aflatoxin B₁ in chili samples using ATR–FTIR coupled with machine learning algorithms. We found that 83.6% of the chili powder samples were contaminated with Aspergillus and Penicillium species, with aflatoxin B₁ levels ranging from 7.63 to 44.32 µg/kg. ATR–FTIR spectroscopy in the fingerprint region (1800−400 cm^-1) showed peak intensity variation in the bands at 1587, 1393, and 1038 cm^-1, which are mostly related …

The Role And Mechanism Of Cinnamaldehyde In Cancer, Jiahua Peng, Xin Song, Wenbin Yu, Yuhan Pan, Yufei Zhang, Hui Jian, Bin He

Journal of Food and Drug Analysis

As cancer continues to rise globally, there is growing interest in discovering novel methods for prevention and treatment. Due to the limitations of traditional cancer therapies, there has been a growing emphasis on investigating herbal remedies and exploring their potential synergistic effects when combined with chemotherapy drugs. Cinnamaldehyde, derived from cinnamon, has gained significant attention for its potential role in cancer prevention and treatment. Extensive research has demonstrated that cinnamaldehyde exhibits promising anticancer properties by modulating various cellular processes involved in tumor growth and progression. However, challenges and unanswered questions remain regarding the precise mechanisms for its effective use as …

Trends And Current Food Safety Regulations And Policies For Functional Foods And Beverages Containing Botanicals, Jarunee Intrasook, Takuji W. Tsusaka, Anil Kumar Anal

Journal of Food and Drug Analysis

Globally, the demand for functional foods and beverages has significantly increased due to socioeconomic changes, particularly in health consciousness to enhance their functionality. Functional ingredients derived from botanicals are widely used because of their phytochemical properties with health benefits. This study aims to (1) review the capabilities and challenges of botanical addition in functional foods, (2) review current policies and regulations for functional foods containing botanicals in the European Union (EU), Canada, Japan, the Republic of Korea, and Thailand, and (3) provide recommendations on effective food safety control measures for better consumer trust and trade facilitation. This critical review was …

40 Years After The Moratorium On Commercial Whaling: Assessing The Competence Of The International Whaling Commission To Confront Critical Threats To Cetaceans, Chris Wold

Pace International Law Review

With Japan’s withdrawal from the International Convention for the Regulation of Whaling (ICRW), no member of the International Whaling Commission (IWC) hunts whales commercially except by objection or reservation, thus intensifying the debate over what the IWC should do and what it may do. For decades, some IWC members have questioned the mandate of the IWC to manage small cetaceans, regulate whaling in coastal State exclusive economic zones, and make recommendations concerning entanglement, bycatch, and other threats to whale conservation. An analysis of the ordinary meaning of the ICRW and the practice of the IWC shows that the IWC has …

La Condition Féminine En Algérie Et Sa Transcription Filmique Chez Nadia Zouaoui, Saïd Adel

Journal of Amazigh Studies

N/A

Répertoire Kabyle Culturel Immatériel Ou Comment (Re) Penser L’Oralité : De La Fixation Écrite Au Numérique, Katia Bellal

Journal of Amazigh Studies

L’oralité reste l’outil incontournable de la préservation et de la transmission du répertoire culturel et immatériel amazigh. Après avoir conquis l’écrit, la tradition orale a su se renouveler grâce aux nouvelles technologies. Les plateformes numériques telles que les réseaux sociaux comme YouTube donnent accès à une représentation numérique de la culture orale amazighe. Ces plateformes numériques incarnent de nouvelles opportunités de partage de cet héritage immatériel à travers les médias sociaux. Cet article explore cette nouvelle expérience de l’oralité, ses manifestations, et ses conséquences, tel que par exemple le fait de s’affranchir de certains tabous et traditions.

Mots-clés : Oralité …

Tuareg Women’S Writing: The Works Of Zakiyatou Oualett Halatine, Cheryl Toman

Journal of Amazigh Studies

In 2013, Tuareg and Malian writer, Zakiyatou oualett Halatine, was forced to flee a conflict that rages on in Mali to this day, and her creative work, Passions du désert, along with a collection of proverbs and a book-length essay, became a means of reconstructing memory and identity of a people ultimately blamed for Mali’s divisions. Zakiyatou’s texts provide us with a rare look at the desert region of Northern Mali from a woman’s perspective. Zakiyatou’s writings are unique in a corpus of Malian literature mostly generated by Southern Malian authors. In many ways, she is the lone literary …

Orality As Aesthetic Device: Articulating Language, Religion, And Authority In Mohamed Akounad’S Tawargit D Imik, Mohamed El Jarari, Salaheddine Belarbi

Journal of Amazigh Studies

This article explores Mohamed Akounad's novel Tawargit d imik, which focuses on protagonist Ssi Brahim who struggles to convey the essence of his religious sermons to an exclusively amazigh audience amidst societal opposition. By examining Ssi Brahim's character as the author's alter ego, the study aims to assess the novel's portrayal of religious concerns and activism for Tamazight and identity, particularly in relation to orality. Employing a sociolinguistic approach drawing from Bourdieu's Language and Symbolic Power, the analysis delves into the intersection of religion, authority, and language, placing orality at the heart of their role within the narrative. This …

The 2024 International Journal Of Exercise Science Position Stand On Inclusion, James W. Navalta, Dustin W. Davis, Jafra Thomas, Matthew J. Garver, Shannon Siegel, Joel D. Reece, Jill M. Maples

International Journal of Exercise Science

International Journal of Exercise Science 17(8): 730-749, 2024. The International Journal of Exercise Science (IJES) Working Group for Opportunity, Representation, and Diverse Perspectives was tasked with developing a position stand to provide guidance in critical areas of inclusion. We present to authors, reviewers, and all readership IJES ideals to utilize in practice. Key points are presented, followed by a deeper look with greater explanation into the areas of inclusive language, diversity and inclusion in research, and accessibility. The same approach is presented for the areas of age, disability status, gender identity and sexual orientation, race, and ethnicity with attention to …

Radiometric Nmi-On-A-Chip: Predictable Spectral Response Scale From 200 Nm - 1050 Nm, Jarle Gran, Trinh Tran, Johanne Solheim

The Characterization and Radiometric Calibration for Remote Sensing Annual Meeting (CALCON)

No abstract provided.

The Secrets Of Christian Others: Hungarian Catholic Intellectuals Debate Ecumenism At A Transylvanian Pilgrimage Site, Marc Roscoe Loustau

Journal of Global Catholicism

Claims about a shared Christian tradition animate European debates about religious otherness, but more remains to be known about how Catholics on Europe’s near-margins understand ecumenical unity among churches. I analyze contemporary Hungarian Catholic intellectuals’ publications about a controversy at the Hungarian national shrine, Our Lady of Csíksomlyó, in Transylvania. When a priest wrote that Csíksomlyó’s annual pilgrimage commemorated sixteenth-century Catholics’ victory over an invading Unitarian army, Transylvania’s Unitarian bishop denounced the origin as an undocumented myth. Prominent Catholic ethnologists, historians, and theologians agreed that, in the name of ecumenism, intellectuals should not publicly mention the origin narrative. But they …

Meeting Or Beating Gendered Expectation During The Holocaust, Katarina Andersen

Chronos

No abstract provided.

The Origin And Lifespan Of Food Assistance Programs During The Great Depression, Vincet Gottfried Wisehoon

Chronos

No abstract provided.

Chronos Volume 10 Issue 1- Full Issue

Chronos

No abstract provided.

The Wind Rises: A Swirling Controversy, Sean Mowry

Chronos

No abstract provided.

Letter From Jacksonville Humane Society To Friends Of Animals, Winthrop Bancroft

Eartha M. M. White Textual Material

Correspondence: Form letter from Winthrop Bancroft, President of Jacksonville Humane Society soliciting donations for the society. Undated.

Noda News December 2010

New Orleans Dental Association News

New Orleans Dental Association (NODA) News includes regular columns with regional news impacting the New Orleans membership including: columns from the President and Executive Director, feature articles relating to the field of dentistry, association events and upcoming activities, component news, continuing education opportunities, and classifieds.

Socialization Procedure For An Adult Feral Cat In A Home Setting, Erica Serito

Thesis Projects

Of the nearly sixty million feral cats, most are deemed unadoptable and thereby unable to be rescuable given their aggressive behaviors towards humans. There are currently no evidence-based socialization training procedures used to socialize adult feral cats with the main interventions for the fearful adult feral cats being trap-neuter-return (TNR) or euthanasia. The purpose of the current study was to decrease undesirable behaviors of an adult feral cat to facilitate safe and reciprocal interactions between her and humans. The study evaluated the use of a differential reinforcement of other behavior (DRO) procedure without extinction on an adult feral cat to …

Gh-Twin: Graph Learning Empowered Hierarchical Digital Twin For Optimizing Self-Healing Networks, Nour Moustafa

Sustainable Machine Intelligence Journal

Communication networks are witnessing a fast evolution towards Beyond 5G (B5G), bringing unprecedented complexities and challenges for optimizing networks in guaranteeing self-healing abilities and maintaining quality of services (QoS). To this end, this study presents a Graph Learning-driven Hierarchical Digital Twin framework, called GH-Twin, to build a reliable virtual replica of network components and their communications between different layers, leading to inclusive network representation. The proposed framework introduces graph cross-learning (GCL) distributed across different participants to devise competent predictive modelling of network performance collaboratively and preemptively recognize abnormalities in network settings. To preserve local privacy, differential privacy is applied by …