Digital Commons Network^™

Central Thromboxane A2, Prostaglandin F2Α, Prostaglandin E, And Prostaglandin D Contribute To The Cardiovascular Effects Elicited By Nesfatin-1, Gökçen Güvenç Bayram, Murat Yalçin

Turkish Journal of Medical Sciences

Background/aim: Our recent study revealed that the expression of lipoxygenase (LOX) and cyclooxygenase (COX) enzymes in the hypothalamus is activated by nesfatin-1, leading to the liberation of leukotrienes and prostaglandins (PG), respectively. Moreover, our prior report explained that intracerebroventricular (ICV) nesfatin-1 treatment triggers cardiovascular responses mediated by central LOX and COX enzymes. Building upon our prior reports, the present investigation sought to clarify the role of cardiovascularly active central COX products, such as thromboxane (TX) A2, PGF2α, PGE, and PGD, in orchestrating nesfatin-1-evoked reactions in mean arterial pressure (MAP) and heart rate (HR).Materials and methods: The Sprague Dawley rats, which …

Sting Activation Increases The Efficiency Of Temozolomide In Pten Harbouring Glioblastoma Cells, Zafer Yildirim, Eda Doğan, Hale Güler Kara, Buket Kosova, Vi̇ldan Bozok

Turkish Journal of Medical Sciences

Background/aim: Glioblastoma is one of the most aggressive tumours, resistant to all applied therapy regiments and prone to relapse. Median survival rates are therefore only expressed as months. STING agonists are immunomodulatory molecules that activate type I interferon expression, making them potentially useful in regulating the tumour microenvironment. Since PTEN serves as a critical phosphatase in activating interferon-regulating transcription factors and is frequently mutated in glioblastoma cells, this study aimed to investigate STING activation in glioblastoma cell lines, examining whether they harbour the PTEN protein or not.°Materials and methods: T98G and U118MG glioblastoma cell lines were treated with the 2ʹ3ʹ-c-di-AM(PS)2(Rp,Rp) …

Small Rna Signatures Of Acute Ischemic Stroke In L1cam Positive Extracellular Vesicles, Bharti Manwani, Nivetha Brathaban, Abiya Baqai, Yashee Munshi, Hilda W Ahnstedt, Mengqi Zhang, Kajsa Arkelius, Ted Llera, Edilberto Amorim, Fanny M Elahi, Neel S Singhal

Faculty, Staff and Student Publications

L1CAM-positive extracellular vesicles (L1EV) are an emerging biomarker that may better reflect ongoing neuronal damage than other blood-based biomarkers. The physiological roles and regulation of L1EVs and their small RNA cargoes following stroke is unknown. We sought to characterize L1EV small RNAs following stroke and assess L1EV RNA signatures for diagnosing stroke using weighted gene co-expression network analysis and random forest (RF) machine learning algorithms. Interestingly, small RNA sequencing of plasma L1EVs from patients with stroke and control patients (n = 28) identified micro(mi)RNAs known to be enriched in the brain. Weighted gene co-expression network analysis (WGCNA) revealed small RNA …

Structural Analysis Of The Female Reptile Reproductive System By Micro-Computed Tomography And Optical Coherence Tomography†, Bonnie K Kircher, Michaela A Mccown, Deirdre M Scully, Richard R Behringer, Irina V Larina

Faculty, Staff and Student Publications

Volumetric data provide unprecedented structural insight to the reproductive tract and add vital anatomical context to the relationships between organs. The morphology of the female reproductive tract in non-avian reptiles varies between species, corresponding to a broad range of reproductive modes and providing valuable insight to comparative investigations of reproductive anatomy. However, reproductive studies in reptilian models, such as the brown anole studied here, have historically relied on histological methods to understand the anatomy. While these methods are highly effective for characterizing the cell types present in each organ, histological methods lose the 3D relationships between images and leave the …

Machine Learning In Time-Lapse Imaging To Differentiate Embryos From Young Vs Old Mice†, Liubin Yang, Carolina Leynes, Ashley Pawelka, Isabel Lorenzo, Andrew Chou, Brendan Lee, Jason D Heaney

Faculty, Staff and Students Publications

Time-lapse microscopy for embryos is a non-invasive technology used to characterize early embryo development. This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the extreme gradient boosting algorithm successfully …

Water Quality Assessment, Eutrophication, And Pollution Index In Lake Edku (Nile Delta Of Egypt), Mohamed A. Okbah, Mahmoud S. Ibrahim, Tarek O. Said, Mohammed E.M. Nassar, Maie I. El-Gammal

Blue Economy

Lake Edku is one of the most important lakes in the northern Nile Delta of Egypt. Studying the physicochemical characteristics and nutrient salts is essential for assessing water quality. pH recorded values fell within internationally permitted limits; the temperature was suitable for the growth of fish and living organisms. Electrical conductivity indicates a source of fresh water; salinity indicates that the lake water is brackish; Total Dissolved Solids (TDS) refers to most of the fresh water in the lake. Solid particulate matter occurred within the permissible limits according to WHO, and DO, and some results exceeded the permissible limit. However, …

A Systematic Review On Umbilical Cord As An Effective And Cost-Beneficial Alternative For Stem Cell Therapy, Ashley Janelle R. Barcelona, Frances Claire A. Chin, Hanna Leila S. Manalo

DLSU Senior High School Research Congress

The high cost and limited accessibility of bone marrow-derived stem cell therapy pose substantial challenges to its widespread adaptation. In contrast, the umbilical cord, often discarded as biomedical waste, offers a promising and cost-effective source of stem cells with therapeutic potential. This study aims to investigate the potential cost savings and medical benefits that could be derived from using umbilical cord stem cells as an alternative to bone marrow in stem cell therapy. The study conducted a comparative analysis systematically reviewing existing research using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method. The study compared the procedures, …

The Male Gaze: Textual Analysis On Contemporary Shōnen Anime And How Its Female Characters Are Viewed, Sophia Mikaela A. King, Febe Faith R. Mendoza

DLSU Senior High School Research Congress

With its extensive history, anime continues to create a massive impact all over the globe that would either make or break stereotypes, determining the progression of the particular genre of animation. A significant factor that affects said progression would be the oversexualization of female characters, especially in shōnen anime. Because of this problem, the researchers analyzed in their paper the common patterns of how female characters are portrayed in Assassination Classroom (2015), Kakegurui (2017), and Chainsaw Man (2022), implying the Male Gaze, a theory by Laura Mulvey that examines how movies impact viewers' values by reinforcing pre-existing societal perceptions through …

The Effects Of Mixed Chicken Bone And Crab Shell Meal On The Growth Of Capsicum Annuum (Bell Pepper), Allthur Rudy R. Arroyo, John Rafael V. Capinia, Ethan Jude R. Reyes, Elnathan Jethro B. Sumalbag, Karl Benedict E. Viri

DLSU Senior High School Research Congress

Recycling of nutrient-rich food wastes minimizes reliance on synthetic fertilizers, promotes healthier ecosystems, and soil quality that promotes plant growth. Existing studies have demonstrated the effectiveness of food wastes, such as bone meal (B) and crab shell meal (S), as fertilizers. This study aims to evaluate the effect of varying proportions of B and S fertilizers on the growth of Capsicum annuum (bell pepper), requiring nitrogen, phosphorus, potassium, and calcium. To test the fertilizer, the plants were divided into three groups (100% chicken bone meal, 50% chicken bone meal with 50% crab shell meal, and 100% crab shell meal). Each …

The Force Of Japanese-Styled Gacha Games On Spending Habits Of Selected Students In Metro Manila, Patrick Andrew S. Angeles, Danilo Angelo C. Antonio, Lanz Anghelo B. Gaborno, Ram Miguel C. Liwanag

DLSU Senior High School Research Congress

Gacha games involve using a virtual lottery system tied to its gameplay systems to affect players’ experiences. Due to Gacha games’ lucrative business model of player spending, several studies have been conducted on Gacha games and their connections to player spending habits. With players spending money on these business practices, the study aims to explore the perceptions of senior high school students in Metro Manila regarding the monetization strategies in Gacha games. A quantitative survey was conducted to obtain 82 responses for data collection. Participants answered close-ended questions via Likert-scale to input their perceptions on the Gameplay, Visual Appeals, and …

Streaming Colors Of The Youth: A Textual Analysis Of Lgbtq+ Animated Netflix Original Cartoon Series Of The Generation Alpha, Candice Margareth G. Aguila

DLSU Senior High School Research Congress

With the evolving technological advancements, media can now be consumed on multiple platforms. Netflix is the top platform with the highest number of users, with around 38.1% dedicated to streaming. In addition, LGBTQ+ media consumption has contributed to the increasing number of people identifying as LGBTQ+ in recent times. Ensuring that the next generation of young, impressionable youths would have a better identity formation and social acceptance, it is suggested to create more child-oriented cartoons filled with positive LGBTQ+ representation to pave a path for more inclusive and diverse outlooks – mending the gap of the current disconnection between the …

Human Hair Additive As Thermal-Insulation Enhancement In Food Containers, Jhana Cassandra Mae P. Celebrado, Sophia Mhae T. Jorda, Cheska Erich J. Toliongco

DLSU Senior High School Research Congress

The Philippines is facing challenges due to the worsening climate change, which is presenting significant challenges for food delivery companies in terms of temperature control, spoilage, and delivery delays. To address these issues, researchers have explored the use of human hair waste as a thermal insulation material to improve food insulation. Two setups, with densities of 1.19268 g/cm3 and 2.38537 g/cm3, were created for viability assessments, with a food-grade aluminum thermal insulation pouch serving as the control group. The Hair-Infused Thermal Insulator underwent tests, including thermal conductivity, water absorption, and tensile strength. The results showed a significant difference in temperature …

Potability Assessment Of The Deep Well Water (Tubig Poso) In The Ayta Community Of Barangay Putingkahoy, Rosario, Batangas, Mariane Gail S. Francisco, Ethan Daniel B. Magbatoc, Juliana Ayen C. Provido, Claire Angela Sophia M. Reyes, Mika Stefanie C. Sabularse

DLSU Senior High School Research Congress

This study aims to assess the current state of the water quality and to determine the potability of water of the Ayta community residing in Brgy. Putingkahoy, Rosario, Batangas. Access to clean drinking water remains a critical issue in the Philippines, particularly in indigenous areas. Identifying sources of poor water quality is important in ensuring water safety and protecting community health. Data regarding the deep well water sources used by the tribe were gathered in support of the assessment of the water quality. Water samples were collected from two posos and were sent to Technopark Industrial Laboratory Inc. for microbiological …

Optimizing Water Hyacinth (Eichhornia Crassipes) And Surgical Mask Fiber Ratio For Durability And Flexural Strength, Edrin D. Bustos, Maryam Gwyn A. Estubio, Kaye Angela A. Jarabejo, Myron Isaac R. Magbiray, Juliuz Miguel F. Maravilla, Miuki Felane C. Riano, John Paul D. Verzosa

DLSU Senior High School Research Congress

The study aimed to enhance the durability and flexural strength of fiberglass composites by utilizing water hyacinth and surgical mask fiber ratios, with a focus on reinforcing water hyacinth fibers with polyester-containing surgical mask fibers. Employing a posttest-only controlled group design, the research tested various ratios, including a control group and three experimental groups, to evaluate the mechanical properties of the composites. The results revealed that Experimental 1 (25:25:30) exhibited highest durability (97.33 MPa). Notably, the optimal ratio for the durability was found in Experimental 2 (96.33 MPa) (20:30:50). Moreover, the highest and optimal flexural strength was found in Experimental …

The Correlation Between Students’ Academic Workload And Salivary Ph Levels, Yves Zidane J. Biscocho, Lorenzo Miguel S. Carpio, Giovanni Paolo C. Salceda

DLSU Senior High School Research Congress

Stress has been known to have negative and positive effects on humans. Studies have shown that academic stress, which comes from overwhelming academic demands, causes various negative effects on the well-being of students. Salivary cortisol levels can also be a predictor of academic stress. Concurrently, salivary pH levels are affected by changes in the salivary cortisol, and it can be inferred that salivary pH levels can predict the increase in academic workloads. To prove this relationship between students’ academic workloads and salivary pH levels, the researchers conducted a study utilizing a prospective research design. The data was collected from sixteen …

"And If Thy Right Hand Offend Thee, Cut It Off": Human Sterilization, The Press & Universities, And "The Science Of Surgery" In Oregon, 1904-1940, Audrey Hart

Chronos

No abstract provided.

Chronos Volume 9 Issue 1 - Full Issue

Chronos

No abstract provided.

The Exploration Of An Aegis Amulet With The Head Of A Feline Goddess, Stephanie Breed

Chronos

No abstract provided.

Incorporating Animal-Assisted Therapy (Aat) To Improve Handwriting Skills In Pediatric Occupational Therapy, Jessica M. Simon, Jessica Mchugh

Student Journal of Occupational Therapy

Within the field of occupational therapy, animal-assisted therapy (AAT) is becoming more widely recognized and utilized as a supplement to traditional therapeutic interventions. As this area is still an emerging area of practice within occupational therapy, research is necessary to better understand the effect of AAT on children’s progress toward various goals. The purpose of this research study was to analyze the effectiveness of using Animal-Assisted Therapy to improve children's performance with and engagement in handwriting activities in outpatient pediatric occupational therapy. This study utilized a Quasi-experimental design, assigning participants to two groups. One group participated in handwriting activities while …

Fully Automated Earth Observation Imagery Quality Assurance, Matthias Kolbe, Duy Nguyen, Maurice Schonert, Arne De Wall

The Characterization and Radiometric Calibration for Remote Sensing Annual Meeting (CALCON)

Planet currently operates a fleet of over 200 satellites consisting of medium resolution monitoring SuperDove cubesats and high resolution tasking SkySats, with future very high resolution and hyperspectral tasking missions in development. In terms of image quality, this requires a processing pipeline extracting QA metrics, which is capable of handling these differences to ensure overall image quality stability.

Multiple teams within Planet are involved in our quality assurance concept, starting from system design and the manufacturing process, through payload maintenance, sensor and pixel calibration, and ending with a final quality assessment step independently from the processing pipeline and taking the …

"I Just Have Big Emotions, Okay?!": Exploring Emotional Literacy Through Picture Books, Amie L. Bigelow

Theses and Dissertations

Many children in the United States struggle with mental health issues. The increase in mental health difficulties for children and adolescents has increased so greatly after the COVID19 pandemic that it has been declared a national mental health emergency by the American Academy of Pediatrics, the American Academy of Child and Adolescent Psychiatry and the Children’s Hospital Association (AAP et al., 2021). The burden to provide children with socialemotional learning, opportunities, access, and support often falls on teachers, and this burden can be particularly acute in rural communities, stemming from the limited availability of resources. Using picturebooks through means such …

Viral Peer Advice: Health Memes Used As Speech Acts Of Advising In English, Laurel Smith Stvan

Linguistics & TESOL Faculty Publications & Presentations - Archive

To better represent online health communication modes, an English-language corpus of lay health discourse was expanded to include health-related memes. Using these forms of peer-to-peer lay communication, I propose ways to categorize the illocutionary force of memes when they function as speech acts of advice. The subcorpus contains 200 English-language health-related memes gathered during 2018-2023 through Google image searches. I found the affiliative and disaffiliative strategies that Placencia (2012) noted within Yahoo! Respuestas advice exchanges; with meme multimodality, however, additional versions of these strategies appeared: characters used as sympathetic or encouraging affiliative features, but also chastising or disapproving characters as …

The Aquatic Microbial Environment Shapes The Gut Microbiota, Brain, And Behavior Of Larval Amphibians, Kyle Emerson

Electronic Theses and Dissertations

Microbial communities comprising bacteria, viruses, fungi, and protists live within and on the surfaces of animal hosts. These microbial communities exist in symbiosis with the host, and heavily influence host physiology, development, health, and fitness. Gut-dwelling microbes (i.e., gut microbiota) contribute to host neurodevelopment through a bidirectional Microbiota-Gut-Brain (MGB) axis. Evidence of the MGB axis has been primarily derived from studies that use germ-free (GF) models, which commonly display altered neurophysiology and behavior compared to conventionally raised counterparts. Almost all studies of the MGB axis have used mammalian models in a biomedical framework, leaving a knowledge gap regarding the role …

Non-Transgenic Guinea Pig Strains Exhibit Divergent Age-Related Changes In Hippocampal Mitochondrial Respiration, Maureen A. Walsh, Amanda S. Latham, Qian Zhang, Robert A. Jacobs, Robert V. Musci, Thomas J. Larocca, Julie A. Moreno, Kelly S. Santangelo, Karyn L. Hamilton

Health and Human Sciences Faculty Works

Aim: Alzheimer’s disease (AD) is the most common form of dementia. However, while 150+ animal models of AD exist, drug translation from preclinical models to humans for treatment usually fails. One factor contributing to low translation is likely the absence of neurodegenerative models that also encompass the multi-morbidities of human aging. We previously demonstrated that, in comparison to the PigmEnTed (PET) guinea pig strain which models “typical” brain aging, the Hartley strain develops hallmarks of AD like aging humans. Hartleys also exhibit age-related impairments in cartilage and skeletal muscle. Impaired mitochondrial respiration is one driver of both cellular aging and …

International Human Rights In Asian Constitutions, Ngoc Son Bui

Duke Journal of Comparative & International Law

International law is integrated into national constitutions across the world. Particularly, the convergence of national constitutions with international human rights (IHR) law has been a global trend. Asia has been underexplored in the global scholarship on constitutional convergence. This Article seeks to make both theoretical and empirical contributions by exploring three models of convergence with IHR law in seven Asian constitutions: convergence impelled by international inducement in post-war and post-conflict states (Japan and Cambodia), convergence propelled by the domestic precommitment of new democracies (South Korea and Indonesia), and convergence compelled by the international socialization of the socialist states (China, Laos, …

An Inducible Genetic Tool To Track And Manipulate Specific Microglial States Reveals Their Plasticity And Roles In Remyelination, Kia M Barclay, Nora Abduljawad, Zuolin Cheng, Min Woo Kim, Lu Zhou, Jin Yang, Justin Rustenhoven, Jose A Mazzitelli, Leon C D Smyth, Dvita Kapadia, Simone Brioschi, Wandy Beatty, Jinchao Hou, Naresha Saligrama, Marco Colonna, Guoqiang Yu, Jonathan Kipnis, Qingyun Li

The Brown Foundation: Institute of Molecular Medicine

Recent single-cell RNA sequencing studies have revealed distinct microglial states in development and disease. These include proliferative region-associated microglia (PAM) in developing white matter and disease-associated microglia (DAM) prevalent in various neurodegenerative conditions. PAM and DAM share a similar core gene signature. However, the extent of the dynamism and plasticity of these microglial states, as well as their functional significance, remains elusive, partly due to the lack of specific tools. Here, we generated an inducible Cre driver line, Clec7a-CreERT2, that targets PAM and DAM in the brain parenchyma. Utilizing this tool, we profiled labeled cells during development and in several …

Slow Proliferation Of Bap1-Deficient Uveal Melanoma Cells Is Associated With Reduced S6 Signaling And Resistance To Nutrient Stress, Vivian Chua, Melisa Lopez-Anton, Mizue Terai, Ryota Tanaka, Usman Baqai, Timothy J Purwin, Jelan I Haj, Francis J Waltrich, Isabella Trachtenberg, Kristine Luo, Rohith Tudi, Angela Jeon, Anna Han, Inna Chervoneva, Michael A Davies, Julio A Aguirre-Ghiso, Takami Sato, Andrew E Aplin

Faculty, Staff and Student Publications

Uveal melanoma (UM) is the deadliest form of eye cancer in adults. Inactivating mutations and/or loss of expression of the gene encoding BRCA1-associated protein 1 (BAP1) in UM tumors are associated with an increased risk of metastasis. To investigate the mechanisms underlying this risk, we explored the functional consequences of BAP1 deficiency. UM cell lines expressing mutant BAP1 grew more slowly than those expressing wild-type BAP1 in culture and in vivo. The ability of BAP1 reconstitution to restore cell proliferation in BAP1-deficient cells required its deubiquitylase activity. Proteomic analysis showed that BAP1-deficient cells had decreased phosphorylation of ribosomal S6 and …

Comparing Child Word Associations To Adult Associative Norms: Evidence For Child-Specific Associations With A Strong Priming Effect In 3-Year-Olds, Nadine Fitzpatrick, Caroline Floccia

School of Psychology

Investigating how infants first establish relationships between words is a necessary step towards understanding how an interconnected network of semantic relationships develops in the adult lexical-semantic system. Stimuli selection for these child studies is critical since words must be both familiar and highly imageable. However, there has been a reliance on adult word association norms to inform stimuli selection in English infant studies to date, as no resource currently exists for child-specific word associations. We present three experiments that explore the strength of word–word relationships in 3-year-olds. Experiment 1 collected children’s word associations (WA) (N = 150; female = 84, …

Replication Study On Ostracism And Self-Isolation The Role Of Shame And Guilt, Zhi Quan Lim

Theses and Dissertations

This study explores the ostracism and solitude-seeking link by conceptually replicating the two studies (Ren et al., 2016; 2022) using different methods of inducing the feeling of being ostracized in the participants. In this study, I asked participants to recall a memory of being ostracized to induce the feeling of being ostracized. I am also interested in if shame and guilt mediate this connection. One hundred and thirty-two participants took part in the study. I divided the participants into two groups (the ostracized group and the control group). After finishing the first task, the participants took a modified version of …

Tmprss2 Is A Tumor Suppressor And Its Downregulation Promotes Antitumor Immunity And Immunotherapy Response In Lung Adenocarcinoma, Zhixian Liu, Qiqi Lu, Zhilan Zhang, Qiushi Feng, Xiaosheng Wang

Faculty, Staff and Student Publications

Background: TMPRSS2, a key molecule for SARS-CoV-2 invading human host cells, has an association with cancer. However, its association with lung cancer remains insufficiently unexplored.

Methods: In five bulk transcriptomics datasets, one single-cell RNA sequencing (scRNA-seq) dataset and one proteomics dataset for lung adenocarcinoma (LUAD), we explored associations between TMPRSS2 expression and immune signatures, tumor progression phenotypes, genomic features, and clinical prognosis in LUAD by the bioinformatics approach. Furthermore, we performed experimental validation of the bioinformatics findings.

Results: TMPRSS2 expression levels correlated negatively with the enrichment levels of both immune-stimulatory and immune-inhibitory signatures, while they correlated positively with the ratios …