Digital Commons Network^™

The Protective Efficacy Of A Sars-Cov-2 Vaccine Candidate B1351v Against Several Variant Challenges In K18-Hace2 Mice, Jie Yang, Huifen Fan, Anna Yang, Wenhui Wang, Xin Wan, Fengjie Lin, Dongsheng Yang, Jie Wu, Kaiwen Wang, Wei Li, Qian Cai, Lei You, Deqin Pang, Jia Lu, Changfu Guo, Jinrong Shi, Yan Sun, Xinguo Li, Kai Duan, Shuo Shen, Shengli Meng, Jing Guo, Zejun Wang

Faculty, Staff and Student Publications

The emergence of SARS-CoV-2 variants of concern (VOCs) with increased transmissibility and partial resistance to neutralization by antibodies has been observed globally. There is an urgent need for an effective vaccine to combat these variants. Our study demonstrated that the B.1.351 variant inactivated vaccine candidate (B.1.351V) generated strong binding and neutralizing antibody responses in BALB/c mice against the B.1.351 virus and other SARS-CoV-2 variants after two doses within 28 days. Immunized K18-hACE2 mice also exhibited elevated levels of live virus-neutralizing antibodies against various SARS-CoV-2 viruses. Following infection with these viruses, K18-hACE2 mice displayed a stable body weight, a high survival …

Expression And Secretion Of Sparc, Fgf-21 And Dcn In Bovine Muscle Cells: Effects Of Age And Differentiation, Katie A. Shira, Kara J. Thornton, Brenda M. Murdoch, Gabrielle M. Becker, Gwinyai E. Chibisa, Gordon K. Murdoch

Animal, Dairy, and Veterinary Science Faculty Publications

Skeletal muscle growth is an economically important trait in the cattle industry. Secreted muscle-derived proteins, referred to as myokines, have important roles in regulating the growth, metabolism, and health of skeletal muscle in human and biomedical research models. Accumulating evidence supports the importance of myokines in skeletal muscle and whole-body health, though little is known about the potential presence and functional significance of these proteins in cattle. This study evaluates and confirms that secreted proteins acidic and rich in cysteine (SPARC), fibroblast growth factor 21 (FGF-21), myostatin (MSTN), and decorin (DCN) are …

High Sugar Diet Alters Immune Function And The Gut Microbiome In Juvenile Green Iguanas (Iguana Iguana), Kwanho C. Ki, Erin L. Lewis, Elizabeth Wu, Francis J. Oliaro, Lise M. Aubry, Charles R. Knapp, Karen M. Kapheim, Dale Denardo, Susannah S. French

Biology Faculty Publications

The present work aimed to study whether a high sugar diet can alter immune responses and the gut microbiome in green iguanas. Thirty-six iguanas were split into four treatment groups using a 2x2 design. Iguanas received either a sugar-supplemented diet or a control diet, and either a lipopolysaccharide (LPS) injection or a phosphate-buffered saline (PBS) injection. Iguanas were given their respective diet treatment through the entire study (~3 months) and received a primary immune challenge 1 and 2 months into the experiment. Blood samples and cloacal swabs were taken at various points in the experiment and used to measure changes …

Valorization Of Seafood Waste For Food Packaging Development, Zhijing Zhan, Yiming Feng, Jikai Zhao, Mingyu Qiao, Qing Jin

School of Earth, Environmental, & Marine Sciences Faculty Publications

Packaging plays a crucial role in protecting food by providing excellent mechanical properties as well as effectively blocking water vapor, oxygen, oil, and other contaminants. The low degradation of widely used petroleum-based plastics leads to environmental pollution and poses health risks. This has drawn interest in renewable biopolymers as sustainable alternatives. The seafood industry generates significant waste that is rich in bioactive substances like chitin, chitosan, gelatins, and alginate, which can replace synthetic polymers in food packaging. Although biopolymers offer biodegradability, biocompatibility, and non-toxicity, their films often lack mechanical and barrier properties compared with synthetic polymer films. This comprehensive review …

A Systems Biology-Based Identification And In Vivo Functional Screening Of Alzheimer’S Disease Risk Genes Reveal Modulators Of Memory Function, Adam D Hudgins, Shiyi Zhou, Rachel N Arey, Michael G Rosenfeld, Coleen T Murphy, Yousin Suh

Faculty, Staff and Students Publications

Genome-wide association studies (GWASs) have uncovered over 75 genomic loci associated with risk for late-onset Alzheimer's disease (LOAD), but identification of the underlying causal genes remains challenging. Studies of induced pluripotent stem cell (iPSC)-derived neurons from LOAD patients have demonstrated the existence of neuronal cell-intrinsic functional defects. Here, we searched for genetic contributions to neuronal dysfunction in LOAD using an integrative systems approach that incorporated multi-evidence-based gene mapping and network-analysis-based prioritization. A systematic perturbation screening of candidate risk genes in Caenorhabditis elegans (C. elegans) revealed that neuronal knockdown of the LOAD risk gene orthologs vha-10 (ATP6V1G2), cmd-1 (CALM3), amph-1 (BIN1), …

Robust Quantitative Photoacoustic Imaging For Colorectal Cancer Treatment Monitoring, Sitai Kou

McKelvey School of Engineering Graduate Student Theses & Dissertations

Colorectal cancer is the 2nd leading cause of cancer death in the United States and the incidence among population under 50 years old has been increasing for the past decade. Contrary to obvious vascular and morphological structures in pre-treatment colorectal cancer, during treatment and post-treatment colorectal cancer tissue often contains fibrosis and edema in tumor bed. These complications cause difficulties in evaluating tissue response to neoadjuvant chemoradiation therapy with MRI and endoscopy, leading to unnecessary resection of rectum, which increases healthcare cost and impacts patient quality of life. Photoacoustic (PA) imaging is a promising tool for observing blood distribution in …

Reach, Rachel Immel

Theses

Reach is a 2D digitally animated short film that is dedicated to exploring the sacrifices that are made to achieve our dreams. The story of my graduate thesis film follows Moe, a subterrestrial bat-like creature that dreams of being able to see the sky for the first time. Feeling suffocated by his dark and cramped surroundings, he rushes toward the surface as soon as a busted rusty pipe provides what appears to be an opening to the surface. What he does not know is that he is venturing into danger that will change him forever. This project is inspired by …

Controlled Delivery Of Rosuvastatin Or Rapamycin Through Electrospun Bismuth Nanoparticle-Infused Perivascular Wraps Promotes Arteriovenous Fistula Maturation, Allan John R Barcena, Joy Vanessa D Perez, Marvin R Bernardino, Erin Marie D San Valentin, Jossana A Damasco, Carleigh Klusman, Benjamin Martin, Karem A Court, Biana Godin, Gino Canlas, Natalie Fowlkes, Richard R Bouchard, Jizhong Cheng, Steven Y Huang, Marites P Melancon

Faculty, Staff and Student Publications

In the context of arteriovenous fistula (AVF) failure, local delivery enables the release of higher concentrations of drugs that can suppress neointimal hyperplasia (NIH) while reducing systemic adverse effects. However, the radiolucency of polymeric delivery systems hinders long-term in vivo surveillance of safety and efficacy. We hypothesize that using a radiopaque perivascular wrap to deliver anti-NIH drugs could enhance AVF maturation. Through electrospinning, we fabricated multifunctional perivascular polycaprolactone (PCL) wraps loaded with bismuth nanoparticles (BiNPs) for enhanced radiologic visibility and drugs that can attenuate NIH—rosuvastatin (Rosu) and rapamycin (Rapa). The following groups were tested onto the AVFs of a total …

What’S The N? On Sample Size Vs. Subject Number For Brain-Behavior Neurophysiology And Neuromodulation, Wael F Asaad, Sameer A Sheth

Duncan NRI Faculty and Staff Publications

Neurophysiology and neuromodulation strive to understand the neural basis of behavior through a one-to-one correspondence between a particular brain and its behavioral output. Within this framework, studies with few subjects but sufficient sample sizes can be both rigorous and impactful.

O-Glcnacylation Of Mitf Regulates Its Activity And Cdk4/6 Inhibitor Resistance In Breast Cancer, Yi Zhang, Shuyan Zhou, Yan Kai, Ya-Qin Zhang, Changmin Peng, Zhuqing Li, Muhammad Jameel Mughal, Belmar Julie, Xiaoyan Zheng, Junfeng Ma, Cynthia X Ma, Min Shen, Matthew D Hall, Shunqiang Li, Wenge Zhu

2020-Current year OA Pubs

Cyclin-dependent kinases 4 and 6 (CDK4/6) play a pivotal role in cell cycle and cancer development. Targeting CDK4/6 has demonstrated promising effects against breast cancer. However, resistance to CDK4/6 inhibitors (CDK4/6i), such as palbociclib, remains a substantial challenge in clinical settings. Using high-throughput combinatorial drug screening and genomic sequencing, we find that the microphthalmia-associated transcription factor (MITF) is activated via O-GlcNAcylation by O-GlcNAc transferase (OGT) in palbociclib-resistant breast cancer cells and tumors. Mechanistically, O-GlcNAcylation of MITF at Serine 49 enhances its interaction with importin α/β, thus promoting its translocation to nuclei, where it suppresses palbociclib-induced senescence. Inhibition of MITF or …

A "Prime And Expand" Strategy Using The Multifunctional Fusion Proteins To Generate Memory-Like Nk Cells For Cell Therapy, Niraj Shrestha, Michelle Becker-Hapak, Mark Foster, Ethan Mcclain, Melissa M Berrien-Elliott, Todd A Fehniger, Et Al.

2020-Current year OA Pubs

Adoptive cellular therapy (ACT) using memory-like (ML) natural killer (NK) cells, generated through overnight ex vivo activation with IL-12, IL-15, and IL-18, has shown promise for treating hematologic malignancies. We recently reported that a multifunctional fusion molecule, HCW9201, comprising IL-12, IL-15, and IL-18 domains could replace individual cytokines for priming human ML NK cell programming ("Prime" step). However, this approach does not include ex vivo expansion, thereby limiting the ability to test different doses and schedules. Here, we report the design and generation of a multifunctional fusion molecule, HCW9206, consisting of human IL-7, IL-15, and IL-21 cytokines. We observed > 300-fold …

Task And Stimulus Coding In The Multiple-Demand Network, Sneha Shashidhara, Moataz Assem, Matthew F Glasser, John Duncan

2020-Current year OA Pubs

In the human brain, a multiple-demand (MD) network plays a key role in cognitive control, with core components in lateral frontal, dorsomedial frontal and lateral parietal cortex, and multivariate activity patterns that discriminate the contents of many cognitive activities. In prefrontal cortex of the behaving monkey, different cognitive operations are associated with very different patterns of neural activity, while details of a particular stimulus are encoded as small variations on these basic patterns (Sigala et al, 2008). Here, using the advanced fMRI methods of the Human Connectome Project and their 360-region cortical parcellation, we searched for a similar result in …

Pinpointing Key Dates In Jesus’ Life: His Birth, The Start Of His Ministry, And His Crucifixion, James A. Moseley

Liberty Theological Review

The abundant chronological details embedded in Scripture, along with external time markers in other ancient histories and in astronomical events, persuasively support the view that Jesus was born in mid-June, 2 BC, not on December 25, 4 BC, and that His ministry endured not for three years, as so often claimed, but for 1,350 days or nearly four years, from His baptism on Sunday, 7 Elul, September 2, AD 29 to His ascension on Friday, 26 Iyar, May 13, AD 33.

Echoes Of Eden In Proverbs: Towards A Biblical Theological Ethic, Armen Oganessian

Liberty Theological Review

Abstract:

This study argues for a biblical-theological approach to the ethics of Proverbs. It proposes that the ethical foundation for Proverbs lies in the principles established in Genesis 1-4 The argument unfolds as follows: Tracing the Abrahamic and Davidic covenants, one sees Solomon, the author of Proverbs, as a partial fulfillment of the promised seed in Genesis 3:15. Furthermore, other biblical writers depict Solomon as an Adamic king ruling in an Eden-like context. Consequently, understanding Proverbs' position within redemptive history encourages readers to interpret it through an Edenic framework. In other words, a biblical-theological approach compels readers to interpret Proverbs …

Guilty Pleasures: The Copyright And Labor Of Reality Television, Emily Tracy

Journal of Intellectual Property Law

The Writers Guild of America’s 2023 strike forced audiences to reckon with Hollywood’s labor issues and the powerhouse unions that seek to solve them. Television networks responded with “strike-proof” fall schedules that highlighted a gap in the entertainment industry’s union membership: reality television. This Note examines and questions that gap, focusing on the labor of reality story producers and the important role it plays in creating a copyrightable product. Although copyright doctrine offers story producers little to no protection against exploitation, copyright case law implicitly recognizes story producers as, essentially, writers. This Note uses that case law to argue that …

Another Round For Petrella V. Mgm, Laches, And Raging Bull: Resolving The Circuit Split Over Copyright’S Statute Of Limitations, David E. Shipley

Journal of Intellectual Property Law

There is a split between the Second Circuit and the Ninth and Eleventh Circuits over the interpretation and application of the Copyright Act’s three-year statute of limitations. The disagreement is about whether it bars a copyright infringement plaintiff from recovering for infringing acts occurring outside the statute’s three-year window. The Second Circuit stated in 2020 in Sohm v. Scholastic that the U.S. Supreme Court explicitly delimited damages to the three years prior to the commencement of an infringement action. However, the Ninth Circuit in Starz Entertainment in 2022 and the Eleventh Circuit Nealy v. Warner Chappell Music in 2023 both …

Full Issue: Volume 5, Issue 2, Editorial Board

Swarthmore Undergraduate History Journal

The second issue in the fifth volume of the Swarthmore Undergraduate History Journal.

From “Defilement” To “Modernity”: How Japan's Encounter With The West Brought Beef To The Table, Ying Yi Tan

Swarthmore Undergraduate History Journal

The long Tokugawa – Meiji transition (1850s – 1880s) is arguably one of the most profound socio-economic transitions that Japan underwent in its history. This paper uses beef consumption in Japan as a lens to further investigate the players involved in this transition. Historically more eschewed than other types of meat and tightly associated with the notion of the “other,” beef became an embodiment of progress and modernity during the Meiji Period. Through tracing the historical shift in the Japanese perception of beef, this paper argues that the evolution of beef consumption corresponds to the radically shifting attitudes of the …

Land, Labor, And The Railroad In Industrial Appalachia: How The Baltimore And Ohio Railroad Engineered Space, 1843-1872, Lillian R. K. M. Austin

Swarthmore Undergraduate History Journal

During the nineteenth century, the Baltimore and Ohio railroad reshaped the social, political, and physical environment of the mid-Atlantic around the needs of the industrial economy. The creation of industrial space, and the proliferation of communities to populate it, is seen retrospectively as an inevitable consequence of technological innovation, but engaging with contemporary corporate records reveals that this space, like the railroad, was engineered. In order to design a supply chain that would make industrialization economical, the railroad and coal industries engaged in political and social engineering to design the new locales of industrialism, exemplified in the Allegheny Mountains by …

Climate Change In Arctic And Indigenous Peoples: Challenges And Solutions, Vera Solovyeva

Ocean and Coastal Law Journal

Climate change poses a serious threat to human well-being, negatively affecting health, traditional environmental management, water supply and food security. Changes in the environment are exacerbating indigenous peoples' problems. This is especially relevant to those who lead traditional lifestyles and whose well-being depends on agricultural and livestock production. This Article addresses the challenges and potential solutions to climate change in the Arctic ecosystem, including the Sub-Arctic regions. Physical changes to the landscape are examined alongside impacts on Indigenous culture and identity. In addition, the article explains the importance of Indigenous knowledge, values, and ethics in developing successful adaptation strategies. In …

Greenland's Foreign Policy, Past And Present: From The Merchants' Message To Bilateral Agreements, Kenneth Høegh

Ocean and Coastal Law Journal

No abstract provided.

Kairotic Entanglement: Kairos, Artificial Intelligence, Persuasion, And The Search For Meaning – A Literature Review, Jonathan Moore

Spectra Undergraduate Research Journal

This paper advances to understand kairos in the contemporary context and its relation to emerging persuasive technologies. The ubiquitous and omnipresent nature of digital technologies prompts a deeper understanding of the possible implications of these technologies and the meaning which emerges from human- computer interactions. A substantial literature review was conducted on kairos, captology, and related subterms to understand and interpret the proximal relationships of these concepts. Additionally, a discussion is offered, and a direction for future works is presented. This paper moves to suggest that kairos plays a substantive role in persuasive technologies and human computer interactions by presenting …

Demographic Study Of College Of Sciences At University Of Nevada, Las Vegas, Laura Seo, Nastasija Damjanovic, Angelica Amansec, Aika Dietz, Monika Neda

Spectra Undergraduate Research Journal

In the United States, STEM-related industries are among the fastest-growing. Our study examines the enrollment of students in the College of Sciences (CoS) and the demographics of the student bodies in both undergraduate and graduate programs from 2010 to 2021 at the University of Nevada, Las Vegas (UNLV). Using the method of least squares, a polynomial fit model was used to find trends as well as to calculate enrollment predictions for the years 2025 and 2030. Our results demonstrate that, currently, the UNLV CoS is male dominant in the graduate program and female dominant in the undergraduate program. However, female …

Improving Laboratory Animal Genetic Reporting: Lag-R Guidelines., Lydia Teboul, James Amos-Landgraf, Fernando J Benavides, Marie-Christine Birling, Steve D M Brown, Elizabeth Bryda, Rosie Bunton-Stasyshyn, Hsian-Jean Chin, Martina Crispo, Fabien Delerue, Michael Dobbie, Craig L Franklin, Ernst-Martin Fuchtbauer, Xiang Gao, Christelle Golzio, Rebecca Haffner, Yann Hérault, Martin Hrabe De Angelis, Kevin C Kent Lloyd, Terry R Magnuson, Lluis Montoliu, Stephen A Murray, Ki-Hoan Nam, Lauryl M J Nutter, Eric Pailhoux, Fernando Pardo Manuel De Villena, Kevin A Peterson, Laura G Reinholdt, Radislav Sedlacek, Je Kyung Seong, Toshihiko Shiroishi, Cynthia Smith, Toru Takeo, Louise Tinsley, Jean-Luc Vilotte, Søren Warming, Sara Wells, C Bruce Whitelaw, Atsushi Yoshiki, Asian Mouse Mutagenesis Resource Association, Celphedia Infrastructure, Infrafrontier Consortium, International Mammalian Genome Society, International Mouse Phenotyping Consortium, International Society For Transgenic Technologies, Mutant Mouse Resource And Research Centers, Phenomics Australia, Rrrc-Rat Resource And Research Center, Guillaume Pavlovic

Faculty Research 2024

The biomedical research community addresses reproducibility challenges in animal studies through standardized nomenclature, improved experimental design, transparent reporting, data sharing, and centralized repositories. The ARRIVE guidelines outline documentation standards for laboratory animals in experiments, but genetic information is often incomplete. To remedy this, we propose the Laboratory Animal Genetic Reporting (LAG-R) framework. LAG-R aims to document animals' genetic makeup in scientific publications, providing essential details for replication and appropriate model use. While verifying complete genetic compositions may be impractical, better reporting and validation efforts enhance reliability of research. LAG-R standardization will bolster reproducibility, peer review, and overall scientific rigor.

Assessment Of Patient-Derived Xenograft Growth And Antitumor Activity: The Nci Pdxnet Consensus Recommendations., Funda Meric-Bernstam, Michael W Lloyd, Soner Koc, Yvonne A Evrard, Lisa M Mcshane, Michael T Lewis, Kurt W Evans, Dali Li, Lawrence Rubinstein, Alana Welm, Dennis A Dean, Anuj Srivastava, Jeffrey W Grover, Min J Ha, Huiqin Chen, Xuelin Huang, Kaushik Varadarajan, Jing Wang, Jack A Roth, Bryan Welm, Ramaswamy Govinden, Li Ding, Salma Kaochar, Nicholas Mitsiades, Luis Carvajal-Carmona, Meenhard Herylyn, Michael A Davies, Geoffrey I Shapiro, Ryan Fields, Jose G Trevino, Joshua C Harrell, Nci Pdxnet Consortium, James H Doroshow, Jeffrey Chuang, Jeffrey A Moscow

Faculty Research 2024

Although patient-derived xenografts (PDX) are commonly used for preclinical modeling in cancer research, a standard approach to in vivo tumor growth analysis and assessment of antitumor activity is lacking, complicating the comparison of different studies and determination of whether a PDX experiment has produced evidence needed to consider a new therapy promising. We present consensus recommendations for assessment of PDX growth and antitumor activity, providing public access to a suite of tools for in vivo growth analyses. We expect that harmonizing PDX study design and analysis and assessing a suite of analytical tools will enhance information exchange and facilitate identification …

Preliminary Efficacy Of A Digital Intervention For Adolescent Depression: Randomized Controlled Trial, Emily Peake, Ian Miller, Jessica Flannery, Lang Chen, Jessica Lake, Aarthi Padmanabhan

Psychology

Background:
Adolescent depression is a significant public health concern; however, access to effective mental health care is limited. Digital therapeutics (DTx) can improve access to evidence-based interventions; however, their efficacy in adolescents is sparsely documented.

Objective:
This study aims to examine the efficacy of a mobile app DTx versus an active control as an adjunct treatment for adolescent depression symptoms.

Methods:
An internet-based open-label randomized control trial was conducted nationwide with a partial crossover design, and 168 adolescents aged 13 to 21 years with symptoms of depression were recruited between November 2020 and September 2021. Participants were randomized (1:1) to …

Optimizing Seaweed Biomass Production ‑ A Two Kelp Solution, Michael Stekoll, Alf Pryor, Alexandra Meyer, Hauke L. Kite-Powell, David Bailey, Kendall Berbery, Clifford A. Goudey, Scott Lindell, Loretta Roberson, Charles Yarish

Faculty, Staff, and Students

Interest in farming kelps has grown beyond using kelp for food, feed or biofuels. There is considerable interest in generating biomass from seaweed for use in bioplastics and other products that would substitute for petroleum-derived products. For these uses to be viable, large amounts of biomass are needed. Very large kelp farms can be expensive to build and maintain, leading to the need to optimize the biomass per unit area. Although close spacing of growlines can lead to poor growth, a viable approach may be to grow two species of kelps together: one that hangs down and one that is …

Improving Laboratory Animal Genetic Reporting: Lag-R Guidelines, Lydia Teboul, James Amos-Landgraf, Fernando J Benavides, Marie-Christine Birling, Steve D M Brown, Elizabeth Bryda, Rosie Bunton-Stasyshyn, Hsian-Jean Chin, Martina Crispo, Fabien Delerue, Michael Dobbie, Craig L Franklin, Ernst-Martin Fuchtbauer, Xiang Gao, Christelle Golzio, Rebecca Haffner, Yann Hérault, Martin Hrabe De Angelis, Kevin C Kent Lloyd, Terry R Magnuson, Lluis Montoliu, Stephen A Murray, Ki-Hoan Nam, Lauryl M J Nutter, Eric Pailhoux, Fernando Pardo Manuel De Villena, Kevin Peterson, Laura Reinholdt, Radislav Sedlacek, Je Kyung Seong, Toshihiko Shiroishi, Cynthia Smith, Toru Takeo, Louise Tinsley, Jean-Luc Vilotte, Søren Warming, Sara Wells, C Bruce Whitelaw, Atsushi Yoshiki, Asian Mouse Mutagenesis Resource Association, Celphedia Infrastructure, Infrafrontier Consortium, International Mammalian Genome Society, International Mouse Phenotyping Consortium, International Society For Transgenic Technologies, Mutant Mouse Resource And Research Centers, Phenomics Australia, Rrrc- Rat Resource And Research Center, Guillaume Pavlovic

Faculty, Staff and Student Publications

The biomedical research community addresses reproducibility challenges in animal studies through standardized nomenclature, improved experimental design, transparent reporting, data sharing, and centralized repositories. The ARRIVE guidelines outline documentation standards for laboratory animals in experiments, but genetic information is often incomplete. To remedy this, we propose the Laboratory Animal Genetic Reporting (LAG-R) framework. LAG-R aims to document animals' genetic makeup in scientific publications, providing essential details for replication and appropriate model use. While verifying complete genetic compositions may be impractical, better reporting and validation efforts enhance reliability of research. LAG-R standardization will bolster reproducibility, peer review, and overall scientific rigor.

The Use Of Apparent Diffusion Coefficient Values For Differentiating Bevacizumab-Related Cytotoxicity From Tumor Recurrence And Radiation Necrosis In Glioblastoma, Kamand Khalaj, Michael A Jacobs, Jay-Jiguang Zhu, Yoshua Esquenazi, Sigmund Hsu, Nitin Tandon, Alireza Akhbardeh, Xu Zhang, Roy Riascos, Arash Kamali

Faculty, Staff and Student Publications

OBJECTIVES: Glioblastomas (GBM) are the most common primary invasive neoplasms of the brain. Distinguishing between lesion recurrence and different types of treatment related changes in patients with GBM remains challenging using conventional MRI imaging techniques. Therefore, accurate and precise differentiation between true progression or pseudoresponse is crucial in deciding on the appropriate course of treatment. This retrospective study investigated the potential of apparent diffusion coefficient (ADC) map values derived from diffusion-weighted imaging (DWI) as a noninvasive method to increase diagnostic accuracy in treatment response.

METHODS: A cohort of 21 glioblastoma patients (mean age: 59.2 ± 11.8, 12 Male, 9 Female) …

Post-Immunotherapy Ctla-4 Ig Treatment Improves Antitumor Efficacy, Stephen Mok, Didem Ağaç Çobanoğlu, Huey Liu, James J Mancuso, James P Allison

Faculty, Staff and Student Publications

Immune checkpoint therapies (ICT) improve overall survival of patients with cancer but may cause immune-related adverse events (irAEs) such as myocarditis. Cytotoxic T lymphocyte-associated antigen 4 immunoglobulin fusion protein (CTLA-4 Ig), an inhibitor of T cell costimulation through CD28, reverses irAEs in animal models. However, concerns exist about potentially compromising antitumor response of ICT. In mouse tumor models, we administered CTLA-4 Ig 1) concomitantly with ICT or 2) after ICT completion. Concomitant treatment reduced antitumor efficacy, while post-ICT administration improved efficacy without affecting frequency and function of CD8 T cells. The improved response was independent of the ICT used, whether …