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Studies Of Media To Maximize The Expression Of Bacterial Metalloprotease Genes As Potential Therapeutics For Human Alzheimer’S Disease, Uday Kiran Chigurupati

Honors Capstones

This study investigates the potential of bacterial metalloprotease genes as therapeutic agents for Alzheimer's Disease (AD). The research focuses on Pseudomonas 20ei1 (P. 20ei1), a bacterial species isolated from lagoon water, which contains pitrilysin genes that share similarities with human insulysin. Six pqqL-related genes were identified in P. 20ei1 for this study. The research methodology involved bioinformatic studies, bacterial cell culture in various media, RNA extraction, purification, and cDNA synthesis. Quantitative PCR analysis was performed to analyze gene expression levels in different cDNA samples. The bacteria grown in the medium with insoluble phosphate has shown higher expression of selected genes …

Using Geogebra To Foster Imagination And Creativity To Build Mathematics Confidence In A Stem World, Joseph M. Furner

Transformations

Math teachers can better prepare all student for a STEM world using computer software like GeoGebra to better prepare our youth for such high-tech careers like that of which Einstein was part of while also emphasizing and fostering creativity in the learning of mathematics. It is critical we have young learners who are confident in their ability to do math and be creative. Teachers today need to check dispositions toward mathematics and address math anxiety while building math confidence in their students. In an ever-advancing world of technology, math teachers need to incorporate such emerging technologies like GeoGebra into the …

Rachel’S Saga: Using Adolescent Literature In The Mathematics Classroom As A Form Of Bibliotherapy To Help Students Overcome Math Apprehension, Joseph M. Furner

Transformations

Math anxiety is prevalent and an understanding of mathematics is critical in an increasingly technological/STEM society, teachers must play a role in reducing the levels of math anxiety of learners. More recently, children's and adolescent literature has been recognized now as a means to teaching mathematics to students through the use of stories to make the math concepts relevant and meaningful. Literature may be used as a therapy to reach students who may be frustrated with math and/or who experience math anxiety. Story and picture books such as Math Curse (Scieszka & Smith, 1995) and A Gebra Named Al (Isdell, …

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Transformations

Full Issue

December 10, 2024 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Achillea Millefolium Ameliorates Doxorubicin-Induced Renal Injury Via Inhibition Of Oxidative Stress And Inflammation In Rats, Mohammed Shaiea, Yi̇mi̇ng Dong, Saleh Alomisy, Hassan Al-Mahbashi, Guozhong Zhang, Chuan Wang

Turkish Journal of Medical Sciences

Background/aim: Doxorubicin (Dox) is a potent anticancer medication. Nevertheless, due to nephrotoxicity, its clinical application is restricted. Achillea millefolium (AM) is a traditional medicine used against several disturbances, including kidney disorders. Therefore, this work aims to investigate the preventative properties of AM extract (AME) and their mechanisms against nephrotoxicity caused by Dox in rats. Materials and methods: The rats were assigned randomly to six groups, including a control group, Dox group (5 mg/kg/week via i.p. for 4 weeks), two groups, AME (100 or 200 mg/kg, orally for 28 days), and the last two groups, Dox+ AME (100 or 200 mg/kg, …

399 Portfolio, Addison Miller

Professional Practices (ART 399)

The overall goal of my works is to tell the stories of the characters I have created and give my audience a glimpse into who the characters are and why they are the way that they are. I make pieces and characters based on the environment around me, specifically the plants and animals in my own backyard. My process starts in my current sketchbook. Sometimes characters get reused from a previous time in my life that I feel I could improve upon, characters designs will more likely than not change over the course of time. When I am satisfied with …

African Women Custodians: Teaching, Toponymy And Tacit Reverie In Heritage Education, Tokie Laotan-Brown

Wagadu: A Journal of Transnational Women's & Gender Studies

This paper investigates the critical role of women in African cultures as guardians of cultural heritage. It explores their central function in transmitting traditional ecological knowledge and practices across generations through established pedagogical methods, toponymic knowledge systems (place-names within cultural landscapes), and the cultivation of tacit knowledge. The paper emphasizes the significance of women's contributions to environmental education, often overlooked or undervalued within Western scientific paradigms. This marginalization restricts access to resources and participation in decision-making processes. It argues for the integration of traditional knowledge systems, informed by women's expertise, into formal education and pedagogy. This inclusion serves to validate …

Independent Concentration Extraction As A Novel Green Approach Resolving Overlapped Uv–Vis Binary Spectra And Hptlc-Densitometric Methods For Concurrent Determination Of Levocloperastine And Chlorpheniramine, Ekram Hany Mohamed, Hany Batakoushy, Adel Ibrahim, Zeinab Adel, Marwa Soliman, Sona Abarghahash, Tahanay Mohamed, Fatma Fouad

Pharmacy

Background: The proposed research study introduces independent concentration extraction (ICE) as a novel UV–Vis spectrophotometric approach. The approach can be used for extracting the concentration of two analytes with severely overlapped spectra from their binary mixtures. ICE is based on spectral extraction platform involving simple smart successive methods that can directly extract the original zero order spectra of the analytes at their characteristic (λmax). Chlorpheniramine maleate (CPM) and Levocloperastine fendizoate (LCF) are two commonly co-formulated drugs in cough preparations. The combined mixture was used to confirm the validity of the developed ICE tool. Another less green HPTLC was developed for …

Climate Change Affecting Waterfowl Migration, Destiny Fox

D.U.Quark

Climate Change is a major environmental factor in waterfowl migration patterns. Migratory waterfowl are species of birds that live in or around wetlands, mainly including lakes, streams, and marshes. Contaminated bodies of water at stopover sites during migratory seasons can lead to death among many species of waterfowl, as well as water borne diseases from snail eggs. Changing environmental factors can also lead to negative effects on the bird’s ability to forage properly. These environmental factors include the evaporation of several wetlands that have led to waterfowl foraging in local farmers’ land. Focusing more heavily on improving satellite tracking could …

Self-Perceived Well-Being Among Doctor Of Physical Therapy Students In The United States In 2022 Compared To 2021, David Kietrys, Ellen Zambo Anderson, James S. Parrott, Suchismita Ray

Journal of Wellness

Introduction: The purpose of this study was to explore well-being in a cohort of Doctor of Physical Therapy (DPT) students in 2022, and compare factors related to well-being to a cohort of DPT students from 2021.

Methods: This cross-sectional observational study combined data from a self-administered survey of participants who had participated in a well-being survey in 2021 (and agreed to be contacted for follow-up one year later) with survey data from first year DPT students during the fall of 2022. The survey included the WHO-5 Well-being Index, the Perceived Stress Scale-10, the Brief Resiliency Scale, and an array of …

Angiogenin-Catalyzed Cleavage Within Trna Anticodon-Loops Identified By Cp-Rna-Seq, Megumi Shigematsu, Ryuma Matsubara, Justin Gumas, Takuya Kawamura, Yohei Kirino

Computational Medicine Center Faculty Papers

Angiogenin (Ang), an endoribonuclease belonging to the RNase A superfamily, cleaves the anticodon-loops of tRNAs to produce tRNA-half molecules. Although previous studies have demonstrated the involvement of Ang in the pathobiology of neurodegenerative disorders, the characterization of Ang-generated tRNA halves in neuronal cells remains limited. This is partly due to the technical limitations of standard RNA-seq methods, which cannot capture Ang-generated RNAs containing a 2',3'-cyclic phosphate (cP). In this report, we established an Ang-treatment model using SH-SY5Y, a human neuroblastoma cell line, and demonstrated Ang-dependent accumulation of tRNA halves. By performing cP-RNA-seq, which selectively captures cP-containing RNAs, we identified Ang-generated …

The Oak Springs Biker, Sarah Rogers

Master's Projects

More often than not in teen or young adult-oriented media, the characters and circumstances are ordinarily categorized into archetypes to replicate real-life conditions. Said archetypes in turn periodically portray fabricated or discrediting representations of certain individuals and characteristics, which is what prompted me to revert these representations (specifically those of adolescent characters and contexts) through an artistic, potent deliverable: a screenplay. My screenplay, The Oak Springs Biker, explores numerous adolescents' narratives within a reputable, correctional boarding school, and how these adolescents' lives intersect amid their personal growth and evolvement journeys. Procedurally, I examined six films and their respective screenplays, …

Induction Of Tier 2 Hiv-Neutralizing Iga Antibodies In Rhesus Macaques Vaccinated With Bg505.664 Sosip, Justin C. Smith, Prabhu S. Arunachalam, Traci H. Legere, Lisa A. Cavacini, Eric Hunter, Bali Pulendran, Rama R. Amara, Pamela A. Kozlowski

School of Graduate Studies Faculty Publications

BACKGROUND: A goal of mucosal human immunodeficiency virus type 1 (HIV-1) vaccines is to generate mucosal plasma cells producing polymeric IgA (pIgA)-neutralizing antibodies at sites of viral entry. However, vaccine immunogens capable of eliciting IgA neutralizing antibodies (nAbs) that recognize tier 2 viral isolates have not yet been identified. METHODS: To determine if stabilized native-like HIV-1 envelope (Env) trimers could generate IgA nAbs, we purified total IgA and IgG from the banked sera of six rhesus macaques that had been found in a previous study to develop serum nAbs after subcutaneous immunization with BG505.664 SOSIP and 3M-052 adjuvant, which is …

Arctic Communities In The Face Of Change: Resilience, Governance, And Well-Being, Abigail York, Andrey N. Petrov, Victoria N. Sharakhmatova, Tatiana Degai, Liza Mack, Vera Metcalf, Rodion Sulyandziga, Sean Asikłuk Topkok, Eduard Zdor

Faculty Publications

This chapter brings together insights from Indigenous scholars and experts from the North Pacific, focusing on the Bering Strait region, within a framework emphasizing multi-level governance derived from western academic literature. The chapter highlights a ground-up view of the complexities and challenges Indigenous communities face in this region. Narratives emphasize community experiences in a rapidly changing Arctic with a particular focus on the environment and economy. Yet, there is great diversity in the experiences as well as the threats, emphasizing the need to Indigenize concepts such as environment, economy, governance, and sustainable development. Highlighting local narratives, we explore both unique …

Characterization Of Fresh Bovine Amnion Membrane Combined With Hydroxyapatite As Candidate Scaffold For Alveolar Bone Tissue Engineering, Octarina Octarina, Elly Munadziroh

Journal of Dentistry Indonesia

Objective: This research aims to analyze the characteristics of a BAM-HA biocomposite with ratios of 4:1 and 4:2. Methods: This research is an in vitro laboratory experiment that starts by grinding fresh BAM to produce amnion slurry and then, adding HA powder to the slurry and carrying out freeze-drying on the slurry. Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscope-Energy dispersive X-ray (SEM-EDX) tests are used to analyze the characteristics of the BAM-HA biocomposite. Results: The FTIR test results showed that the BAM-HA biocomposite had amide functional groups I, II, III, A, B, OH, CO_{3 …}

Histomorphometric Assessment Of Vibrational Forces On The Extended Midpalatal Suture, Aylin Pasaoglu Bozkurt, Özge Özdal Zincir, Asli Emincik

Journal of Dentistry Indonesia

Objective: This study determined the effects of vibrational forces on bone formation in the retention period of an expanded mid-palatal, histomorphometric suture in a rat model. Methods: Twenty-four 7–8- to 8-week-old male Wistar rats weighing 190.25 ± 15.35 g were separated into groups: only expansion (G1), expansion and 30 Hz vibration application (G2), and expansion and 111 Hz vibration application (G3). After 5 days of expansion, there were 12 days of retention. All animals were sacrificed, and their premaxilla were dissected. A histological examination evaluated the number of osteoblasts, osteoclasts, and capillaries, the amount of mineralized area, and …

Effectiveness Of Mucoadhesive Patch Clitoria Ternatea Extract In Wound Healing Process After Tooth Extraction In Sprague Dawley Rats, Rahma Ika Amalia, Wahyu Prastiya, Nur Meirawati, Helmi Hirawan, Dwi Nur Indah Sari

Journal of Dentistry Indonesia

Objective: This study aims to evaluate the effectiveness of mucoadhesive patch clitoria ternatea extract for wound healing post-tooth extraction. Methods: The research was carried out experimentally in a laboratory. 30 Sprague Dawley rats were selected and randomly divided into mucoadhesive patch clitoria ternatea (MPCT) 5%, 10%, 15%, positive and negative control groups. The upper incisors of rats were extracted and treated once a day for 7 and 14 days. Wound healing was observed based on histological observation (fibroblasts, collagen density, and epithelial thickness). Data was analyzed statistically. Results: MPCT 15% applied for 14 days showed the highest …

Directed Evolution Of Hedgehog Protein Autoprocessing Activity: Combined Experimental & Computational Approach, Ted Kuke

Undergraduate Honors Theses

Sonic hedgehog protein is vital for embryonic development while also being linked to multiple cancers and holoprosencephaly. The native reaction of hedgehog is a sterolysis reaction whereby cholesterol binds to the HhC catalytic domain and attacks the HhN signaling domain, resulting in a covalent HhN-cholesterol signaling product. The structure of the sterol recognition region (SRR) of Sonic Hedgehog, where cholesterol binds, is currently unknown. In this thesis, I used directed evolution to select a triple mutant of Hedgehog’s catalytic HhC domain, (D46H, Q150H, A196T), which is over 5000 times more catalytically efficient at reacting with epicoprostanol (ECP), a non-native substrate …

Current Status And The Significance Of Local Wisdom Biofertilizer In Enhancing Soil Health And Crop Productivity For Sustainable Agriculture: A Systematic Literature Review, Irwandhi, Fiqriah Hanum Khumairah, Emma Trinurani Sofyan, Nadia Nuraniya Kamaluddin, Anne Nurbaity, Diyan Herdiyantoro, Tualar Simarmata

Jurnal Kultivasi

Soil fertility is recognized as a crucial factor in supporting plant growth and productivity. The utilization of biofertilizers as environmentally friendly fertilizers is aimed at enhancing soil fertility and plant productivity. This study aims to explore the potential of local material for developing local wisdom biofertilizers (LWB) for achieving sustainable agriculture. A systematic literature review was conducted using bibliometric analysis, Preferred Reporting Items for Systematic Reviews, and Meta-Analyses (PRISMA) method, employing the Scopus search engine with the keywords "local AND wisdom AND biofertilizer" OR "biofertilizer" OR "local AND microorganism" OR "soil AND health OR crop AND productivity OR sustainable agriculture". …

Understanding The Transitional Needs Of Servicemembers To Veterans: A Focus On Mental, Social, And Behavioral Health, Marieann C. Raguso

Theses and Dissertations

This applied dissertation was designed to fill the gaps on the transitional American veterans’ lived experience using their own voice. The reconstruction of an American Veteran’s identity after transitioning back into civilian life is a process with many challenges. Transitional practices often fail to help veterans to acculturate back into the civilian world. This includes the formulation of identities and associations. There is a lack of consistency in military transitional practices leading to identity loss and the formulation of mental and behavioral disorders. The aim of this study is to examine the lived experiences of veterans who have transitioned back …

Nonablative Laser Skin Resurfacing Of Fine And Coarse Wrinkles – Two Study Cases, Muna Mustafa, Walid Hamoudi, Khawla Khashan, Qussay Alhashimi, Noor Abdulhameed

Journal of Applied Sciences and Nanotechnology

The use of the Nd+ and Er+: YAG lasers is still under development for cutaneous resurfacing. Cooling the epidermis induces laser heating under invasive epidermal layer regime. Injuring the dermis can treat skin defects and improve its quality. Various therapeutic options have been utilized to treat aging signs. Laser resurfacing has proven effectiveness and popularity due to its shorter recovery time and high success rate. Non-invasive Nd: YAG laser and 2940nm Er: YAG laser pulses were employed to provide deep dermal laser heating and induce elastin and collagen regeneration. Two subjects of skin-tone 2 were laser treated in the present …

Clinical Use Of Bedside Portable Ultra-Low-Field Brain Magnetic Resonance Imaging In Patients On Extracorporeal Membrane Oxygenation: Results From The Multicenter Safe Mri Ecmo Study, Sung-Min Cho, Shivalika Khanduja, Christopher Wilcox, Kha Dinh, Jiah Kim, Jin Kook Kang, Ifeanyi David Chinedozi, Zachary Darby, Matthew Acton, Hannah Rando, Jessica Briscoe, Errol L Bush, Haris I Sair, John Pitts, Lori R Arlinghaus, Audrey-Carelle N Wandji, Elena Moreno, Glenda Torres, Bindu Akkanti, Jose Gavito-Higuera, Steven Keller, Huimahn A Choi, Bo Soo Kim, Aaron Gusdon, Glenn J Whitman

Faculty, Staff and Student Publications

Background: Early detection of acute brain injury (ABI) at the bedside is critical in improving survival for patients with extracorporeal membrane oxygenation (ECMO) support. We aimed to examine the safety of ultra-low-field (ULF; 0.064-T) portable magnetic resonance imaging (pMRI) in patients undergoing ECMO and to investigate the ABI frequency and types with ULF-pMRI.

Methods: This was a multicenter prospective observational study (SAFE MRI ECMO study [Assessing the Safety and Feasibility of Bedside Portable Low-Field Brain Magnetic Resonance Imaging in Patients on ECMO]; NCT05469139) from 2 tertiary centers (Johns Hopkins, Baltimore, MD and University of Texas-Houston) with specially trained intensive …

Micrornas: Where Brilliance, Perseverance, And Ambition Converged, Rares Drula, George A Calin

Faculty, Staff and Student Publications

Victor Ambros at the University of Massachusetts Medical School and Gary Ruvkun at Harvard University were awarded the 2024 Nobel Prize in Medicine and Physiology for the discovery of microRNAs. This “groundbreaking discovery revealed a completely new principle of gene regulation that turned out to be essential for multicellular organisms, including humans” (1). This is a well-deserved and highly anticipated recognition for a seminal discovery published 31 years ago in back-to-back Cell publications (2, 3). This landmark finding changed the way we understand gene expression regulation among most multicellular organisms (4) and had …

Oligosaccharides, Onion Peel, And Essential Oils As Feed Additives In The Diet Of Dairycattle: In Vitro Fermentation, Greenhouse Gases, Ruminal Nutrient Degradability, Andvolatile Fatty Acid Production, Kelechi A. Ike, Oludotun O. Adelusi, Joel O. Alabi, Lydia K. Olagunju, Michael Wuaku, Chika C. Anotaenwere, Deborah O. Okedoyin, Oluteru E. Orimaye, Deandrea Gray, Peter A. Dele, Kiran Subedi, Ahmed E. Kholif, Uchenna Y. Anele

Turkish Journal of Veterinary & Animal Sciences

This study investigated the effect of inclusion of dietary oligosaccharides, onion peel, and an essential oil blend (EOB; containing anise, clove, oregano, cedarwood, and ginger in the ratio 4:2:2:1:1) individually and in combinations in the total mixed ration of dairy cattle on in vitro gas production (GP), dry matter disappearance (DMD), fiber fraction disappearance, and total and individual volatile fatty acid (VFA) production. EOB was used at 200 μL g–1 of feed while the other additives were administered at 3% of the feed on a dry matter basis. The in vitro batch culture technique was used for this experiment and …

Honeybee Biodiversity Based On Five Mitochondrial Genes And New Evidence For Differentsublineages In Türkiye, Meral Kekeçoğlu, Songül Bi̇r, Merve Kambur Acar, Rustem Ilyasov

Turkish Journal of Veterinary & Animal Sciences

In this study, the genetic structure of honey bee populations in Türkiye was analyzed using eleven restriction endonuclease enzymes targeting the COI-COII, COI, 16S rDNA, ND5, and CYT B genes. A total of 553 worker honey bee samples were collected from 43 different districts across Türkiye, as well as from Greece, Bulgaria, and Georgia, neighboring countries of Türkiye. Analysis of the COI region using the HinfI restriction enzyme revealed the presence of two mitotypes. Type 1 was the predominant type in Türkiye, while Type 2 was detected exclusively in Hatay, a province in the southern part of the country. Notably, …

Phenotypic And Molecular Characterization And Antimicrobial Susceptibility Pattern Of Pasteurella And Mannheimia Species Obtained From Cattle And Sheep With Pneumonia, Eli̇f Karadeni̇z Pütür, Fati̇h Büyük, Yaren Ersoy

Turkish Journal of Veterinary & Animal Sciences

Among the heterogeneous Pasteurellaceae family, Pasteurella multocida and Mannheimia haemolytica are the pathogenic species that cause enzootic pneumonia in cattle and sheep. This study aimed to characterise Pasteurella and Mannheimia species obtained from pneumonia cases in cattle and sheep. 100 cattle and 100 sheep lungs diagnosed with infectious pneumonia as a result of histopathological examination were analyzed for Pasteurellaceae members by phenotypic methods, VITEK 2 Compact system and PCR. Antibiotic susceptibilities and phylogenetic analysis of the isolates were done by VITEK 2 Compact System and 16S rRNA-PCR, respectively. Seven Pasteurella multocida and 9 Mannheimia haemolytica were identified. All strains were …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

International Methods Of Offender Profile Construction, Standardization, And Effective Criminal Investigative Processes, Savannah Peoples

Walden Dissertations and Doctoral Studies

Offender profiling is a tool used within criminal investigations to infer offender characteristics according to evidence collected from a crime scene. The practice of offender profiling has been applied since the Roman era, empirically developed, and recently popularized in media depictions. Existing studies demonstrate that there is a lack of peer reviewed literature that examines current methods of criminal profile construction, opportunities for standardization, and effective criminal investigative processes. Motivation for this study came after reviewing research that revealed high rates of serious and violent international serial offending, noted as a major challenge for law enforcement agencies, and a significant …

International Methods Of Offender Profile Construction, Standardization, And Effective Criminal Investigative Processes, Savannah Peoples

Walden Dissertations and Doctoral Studies

Offender profiling is a tool used within criminal investigations to infer offender characteristics according to evidence collected from a crime scene. The practice of offender profiling has been applied since the Roman era, empirically developed, and recently popularized in media depictions. Existing studies demonstrate that there is a lack of peer reviewed literature that examines current methods of criminal profile construction, opportunities for standardization, and effective criminal investigative processes. Motivation for this study came after reviewing research that revealed high rates of serious and violent international serial offending, noted as a major challenge for law enforcement agencies, and a significant …