Digital Commons Network^™

Mirna-Target Gene Network Analysis In Siblings With Cystic Fibrosis And Phenotypic Variability, Ayberk Mustafaoğlu, Senem Noyan, Yeli̇z Z. Akkaya Ulum, Bala Gür Dedeoğlu, Nagehan Emi̇rali̇oğlu, Uğur Özçeli̇k, Ebru Yalçin, Deni̇z Doğru, Nural Ki̇per, Di̇dem Dayangaç Erden

Turkish Journal of Medical Sciences

Background/aim: Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CF is characterized by respiratory tract infections, pancreatic insufficiency, meconium ileus, intestinal obstruction, and male infertility. A genotype to phenotype correlation is difficult to establish because of the heterogeneity of disease severity. Even patients with the same CFTR mutation can have varying clinical severities. In recent years, studies have explored the role of microRNA (miRNA) expression in the regulation of respiratory diseases. However, no research has been conducted to date on miRNAs in siblings with the same CFTR mutation. …

Analyzing The Global Changes In The Seasonal Cycle Of Sea Level, Storm Surge, And Ocean Waves, Amanda Barroso

Graduate Thesis and Dissertation post-2024

Changes in the seasonal cycle of extreme sea level components can modulate the flooding risk along coastlines. This dissertation evaluates the changes in and potential co-occurrence of the seasonal cycles of mean sea level, storm surge, and ocean waves. Seasonal cycles characterize the generally expected climate conditions throughout the year with distinct high and low patterns. These regular and predictable cycles have shown non-stationarity in some locations across the globe. This means the timing of the expected peak may shift to occur earlier or later. Shifts in the timing of the maximum sea level impact coastlines with increased risk of …

Morphology, Evolution And Host-Driven Convergence In Ichneumonidae (Hymenoptera, Ichneumonoidea), Davide Dal Pos

Graduate Thesis and Dissertation post-2024

Understanding the functional and evolutionary significance of morphology is fundamental to interpreting the tree of life. In recent years, emerging tools have enabled broad-scale analysis of discrete morphological data and facilitated the study of entire phenomes, helping to re-establish morphology as a central component of evolutionary research. These advances allow us not only to examine individual traits in detail but also to compare them across taxa and explore their evolutionary trajectories. This approach is particularly critical when studying hyperdiverse groups that exhibit both high levels of morphological convergence and complex life cycles intricately tied to host use. A prime example …

Rna’S Symphony: Harmonizing Splice Junctions And Exon Counts For A Novel Approach To Differential Splicing Analysis, Jelard Aquino

UNLV Theses, Dissertations, Professional Papers, and Capstones

Alternative Splicing (AS) plays a critical role in transcriptome complexity and cell-type-specific gene regulation, yet its analysis remains methodologically fragmented, especially in the context of noisy and sparse single-cell RNA sequencing (scRNA-seq) data. This dissertation addresses key computational challenges in AS detection by evaluating existing tools, developing integrative frameworks, and proposing new strategies for improving analysis accuracy in both bulk and single-cell contexts. In chapter 1, I present a comprehensive literature review of computational tools designed for detecting and quantifying AS from bulk and scRNA-seq data. This review outlines major methodological paradigms, including exon-based and splice junction-based approaches, and evaluates …

Functional Biopolymers Applied To Sustainable Technologies In The Environment And Healthcare, Fengjie He

UNLV Theses, Dissertations, Professional Papers, and Capstones

Biodegradable polymeric materials (biopolymers) are naturally derived materials known for their excellent biocompatibility, biodegradability, sustainability, and versatile chemical functionality. They have attracted increasing attention in various applications as alternative to synthetic materials ranging from food packaging to tissue engineering. Meanwhile, with intrinsic advantages, biopolymers have also emerged as promising materials in addressing contemporary challenges in both biomedical and environmental fields. Motivated by the significant potential of biopolymers and the growing need for sustainable materials, my research focuses on the design and engineering of biodegradable polymers with novel modification methods and application directions. In this work, two representative biopolymers are selected: …

Housewerk: How Drag Performers Engage In Emotional Labor While Navigating Community Care, Steph O. Landeros

UNLV Theses, Dissertations, Professional Papers, and Capstones

This dissertation examines drag performance as a form of service labor shaped by emotional labor, community care, and identity-based inequality. Drawing on interviews with drag performers shows how drag labor blends artistic skill, emotional regulation, interpersonal care, and entrepreneurial labor. While these demands mirror service sector roles in other industries, drag remains underrecognized and undervalued, particularly for performers who do not conform to white, cisgender norms. The study introduces Housewerk—an adaptation of Marlon Bailey’s concept of housework (Bailey, 2013)—to describe the mix of paid and unpaid labor drag performers engage in. Housewerk captures how performers must balance self-preservation (branding, income, …

An Analysis Of The Influence Of Human Disease Pathogens On Core Behaviors Of The Black-Legged Tick, Emily F. Marinko

UNLV Theses, Dissertations, Professional Papers, and Capstones

Ixodes scapularis, the black-legged or deer tick, is a vector for several different disease-causing microbes, including the causative agent of Lyme disease. As such, it is a major health concern for people living within its range in the eastern half of the United States, a habitation area that is increasing due to climate change. Despite its medical relevance as a disease vector, there is still much to learn about the basic behavior of these arachnids in the wild. Thus, the major hypothesis of this thesis is that ticks that are infected with specific human disease-causing microbes behave differently than those …

A Chronic Acinetobacter Baumannii Pneumonia Model To Study Long-Term Virulence Factors, Antibiotic Treatments, And Polymicrobial Infections, Clay D Jackson-Litteken, Gisela Di Venanzio, Manon Janet-Maitre, Ítalo A Castro, Joseph J Mackel, Leslie D Wilson, David A Rosen, Carolina B López, Mario F Feldman

2020-Current year OA Pubs

Acinetobacter baumannii causes prolonged infections that disproportionately affect immunocompromised populations. Our understanding of A. baumannii respiratory pathogenesis relies on an acute murine infection model with limited clinical relevance that employs an unnaturally high number of bacteria and requires assessment of bacterial load at 24-36 h post-infection. Here, we demonstrate that low intranasal inoculums in tlr4 mutant mice allows for infections lasting at least 3 weeks. Using this "chronic infection model" we determine the adhesin InvL is a virulence factor required during later stages of infection, despite being dispensable in the early phase. We also demonstrate that the chronic model enables …

Highly Potent And Subtype-Selective Sperm-Specific Potassium Channel Slo3 Inhibitors Display High Tissue Exposure In The Murine Female Reproductive Tract, Kayla J Temple, Ping Li, Maximilian D Lyon, Shweta Bhagwat, Leila Asadi, Sophia Li, Ronald Mccarthy, Celia M Santi, Et Al.

2020-Current year OA Pubs

To date, the study of drug exposure and distribution throughout the female reproductive tract has often been overlooked and neglected. Herein, we report the first highly potent and subtype-selective sperm-specific potassium channel 3 (SLO3) inhibitors as a new modality of contraceptives. After extensive

Claudin-1-Mediated Signaling And Therapy Resistance In Colorectal Cancer: From Mechanism To Translation, Mark W. Primeaux

Theses & Dissertations

Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, with metastatic cases showing poor response to chemotherapy due to both intrinsic and acquired therapy resistance. Claudin-1 (CLDN1), a tight junction protein, is overexpressed and mislocalized outside of tight junctions in CRC, where it contributes to an aggressive, metastatic phenotype. Although this causal relationship has been well established, the mechanisms by which CLDN1 promotes tumor progression were unclear due to its lack of intrinsic enzymatic activity. This dissertation investigates the molecular mechanisms through which CLDN1 drives oncogenic signaling, its role in therapy resistance, and its translational potential as both a …

Restoring Translational Control With Novel Small Molecule Activators Of Pp2a In Cancer Cells, Kayla A. Jonas

Theses & Dissertations

Hyperactivation of eIF4E-dependent translation in tumor cells is required for robust expression of oncogenic proteins and maintenance of the transformed phenotype. eIF4E binds to the 7-methylguanylate structure at the 5’ end of cellular mRNAs and nucleates the formation of the translation initiation complex, eIF4F, which recruits the preinitiation complex and resolves secondary structure in the mRNA. The ability of eIF4E to promote eIF4F assembly is negatively regulated by the translational repressor, 4E-BP1, and tumorigenesis depends on functional inactivation of 4E-BP1. A major mechanism of 4E-BP1 inactivation involves inhibitory phosphorylation by mTOR which prevents eIF4E binding. Functional inactivation can also occur …

Scientist Perspectives: A Correlation Analysis Of Translational Science Competencies Compared With Principles Of Engagement And Knowledge Integration, Rachel Wenzl

Theses & Dissertations

The competencies identified in the development of the “Fundamental Characteristics of the Translational Scientist” may not consider and fully integrate the concepts of knowledge integration and engagement, key components of the translational science spectrum. This gap may be widening and deepening the “valley of death” and exacerbating ongoing challenges in the field of translational science. This dissertation explored the association of knowledge integration, stakeholder engagement, and community engagement principles compared with translational science competencies.

Utilizing content review and a non-experimental, correlation study design, data were collected through a quantitative survey from adult research subjects all self-identified as a research investigator …

Beyond The Bruise: Understanding Injury Risk Factors Among Livestock Workers In Cattle Feedyards And Animal Handling Farms And Ranches, Rishad Ahmed

Theses & Dissertations

Cattle feeding and livestock production are central to U.S. agriculture and represent some of the most hazardous occupational environments. Workers in feedyards and on cattle farms and ranches face elevated risks of injury due to physically demanding labor, frequent animal handling, and exposure to environmental and ergonomic hazards. Many feedyard workers are Hispanic/Latino immigrants who may experience additional barriers related to language, healthcare access, and job security. Similarly, injury risks among cattle producers are compounded by seasonal workload fluctuations, limited rest opportunities, and psychosocial stressors. Beyond individual impacts, agricultural injuries also pose a substantial economic burden. Recent estimates indicate that …

Transcriptomic And Epigenomic Profiling Of Immune Cells Responses To Simian Immunodeficiency Virus Infection In The Brain, Xiaoke Xu

Theses & Dissertations

Human immunodeficiency virus type 1 (HIV-1) is an enveloped retrovirus that significantly impairs the human immune system. A common complication of HIV-1 infection is HIV-associated neurocognitive disorder (HAND), which, prior to the introduction of antiretroviral therapy (ART), led to considerable morbidity and mortality due to untreated neuropsychiatric manifestations. Although ART has substantially reduced the incidence of HIV-associated dementia (HAD), approximately 50% of people living with HIV (PLWH) continue to experience milder forms of HAND. The CNS is seeded by HIV during the acute phase of infection. Once in the brain, HIV targets and activates resident immune cells, primarily microglia and …

The Characterization Of Per- And Polyfluoroalkyl Substances (Pfas) In Nebraska Drinking Water And Human Health Impacts, Sarah Tucker

Theses & Dissertations

Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds that have been detected in water sources of urban and rural environments with evidence of adverse human health effects from exposure. Humans are primarily exposed through ingestion of contaminated water and food. PFAS exposure has been linked to negative multi-system health outcomes (liver, kidneys, cardiovascular, reproductive, and immune). PFAS occur as persistent mixtures in environmental media with decades long consequences to water quality. While national agencies have launched large-scale PFAS water quality monitoring studies, limited data exists for contamination of rural water in Nebraska, especially private wells. The aims of this study …

Temporal Patterns Of Ectopic Pregnancy: Using The Electronic Health Record To Illuminate A Dark Corner In Women's Health, Kirsten Hepburn

Theses & Dissertations

Ectopic pregnancy remains a significant contributor to pregnancy-related morbidity and mortality, yet little is known about whether its occurrence follows discernible temporal patters that could inform prediction and prevention efforts. This dissertation aimed to explore the temporal characteristics of ectopic pregnancy through three interrelated projects: development of a new theoretical framework, a scoping review of the literature on ectopic pregnancy temporal patterns, and an empirical study using electronic health records data from a ten-year period at a single academic medical center. The Nursing Genomic and epigenomic, Environment, and Microbiome (N-GEM) situation-specific theoretical framework was introduced as a novel nursing theory …

Role Of Stabilin-1 Expressing Macrophages In Colorectal Liver Metastasis, Jampa L. Gurung

Theses & Dissertations

Colorectal cancer (CRC) is the 2nd most common cause of cancer-related mortality, primarily due to its spread to distant organs. Colorectal liver metastasis (CRLM) is the foremost form of CRC spread due to the anatomical link between the liver and intestine. Despite advancements in diagnostic tools and adjuvant therapies, the survival rate of CRLM remains low, indicating the significant need to identify targetable mechanisms. Stabilin-1 (STAB1), a scavenger receptor expressed on tumor-associated macrophages, has been linked to tumorigenesis and poor outcomes in various cancers. However, the role of STAB1 in CRLM is not known. Here, we investigated the role of …

Sox2 Regulates Foregut Squamous Epithelial Homeostasis And Is Lost During Barrett's Esophagus Development, Ramon U. Jin, Yuanwei Xu, T. Mamie Lih, Yang-Zhe Huang, Toni M. Nittolo, Blake E. Sells, Olivia M. Dres, Jean S. Wang, Qing K. Li, Hui Zhang, Jason C. Mills

2020-Current year OA Pubs

Esophageal adenocarcinoma is increasingly prevalent and is thought to arise from Barrett's esophagus (BE), a metaplastic condition in which chronic acid and bile reflux transforms the esophageal squamous epithelium into a gastric-intestinal glandular mucosa. The molecular determinants driving this metaplasia are poorly understood. We developed a human BE organoid biobank that recapitulates BE's molecular heterogeneity. Bulk and single-cell transcriptomics, supported by patient tissue analysis, revealed that BE differentiation reflects a balance between SOX2 (foregut/esophageal) and CDX2 (hindgut/intestinal) transcription factors. Using squamous-specific inducible Sox2-KO (Krt5CreER/+ Sox2Δ/Δ ROSA26tdTomato/+) mice, we observed increased basal proliferation, reduced squamous differentiation, and expanded metaplastic glands at …

Learning Structure With Multivariate Information Bottleneck And Exploration Of New Methods In Sequential Decision Making, Volodymyr Makarenko

Master's Theses

Research in useful information extraction has been motivated by the increasing demand to extract insights from unstructured data, and by the need to store and transmit great volumes of information, often originating in unstructured data such as videos. Research in rate distortion and information bottleneck paved the path for understanding and guiding the design of lossy encoders, capable of extracting relevant information. Independently, research in deep representation learning has enabled numerous applications for unstructured high-dimensional data such as images. However, the interpretability of the deep learning methods remained limited. Several desired properties of learned representations have been suggested, including disentanglement. …

Health Tourism Facility Use Of Digital Marketing And Ai, David Vequist Iv

Visions in Leisure and Business

The proliferation of social media platforms has fundamentally reshaped patient acquisition strategies in health and wellness tourism, creating unprecedented opportunities for providers to engage with international patients across diverse digital channels. From boutique clinics in Mexico offering specialized dental, cosmetic, and bariatric procedures, to destination medspas providing aesthetic treatments, and even comprehensive global hospitals like the Mayo Clinic, healthcare providers are leveraging multi-platform social media strategies to attract and inform potential medical travelers. Research indicates that these digital marketing approaches now encompass a sophisticated array of content formats, including short-form video on TikTok, professional imagery on Instagram, long-form educational content …

Evaluating Nfl Quarterback Decision Making Through Tracking Data, Jesse N. Melville

Theses and Dissertations

Quarterback decision-making is one of the most critical determinants of success in the National Football League (NFL), yet traditional statistics fail to account for the spatial, temporal, and contextual factors influencing each play. This thesis proposes a data-driven methodology for evaluating quarterback decisions using NFL player tracking data from the 2022 season. We replicate and extend the DeepQB framework by training deep learning models to estimate expected completion probabilities and expected yards gained for each receiver at the moment of the throw. Our extension applies the approach to a newer dataset with additional contextual features, enabling a more detailed evaluation …

Flowing Progress: Transforming The Danube Through Infrastructure, Stefan Dorondel, Luminita Gatejel

Central European Studies

Flowing Progress: Transforming the Danube Through Infrastructure focuses on how different political regimes and forms of governance have imagined and technologically transformed the most international river in the world. Multidisciplinary and drawing on methodologies of history, anthropology of infrastructure, and science, technology, and society, this collection explores the tensions between the river and its natural pulses, the humans that populate its floodplains, state agencies, and infrastructure. The book engages the concept of disturbance to point out the circular and spiraling dynamics between hydrological processes and technopolitical and economic practices. Disturbance denotes a specific type of long-term dynamic between human attempts …

Hydrothermal Synthesis Of High Surface Area Mesoporous Silica As An Efficient Adsorbent For Removal Of Crystal Violet Dye From Aqueous Solution, Inaam H. Ali, Ameera Hassan Hamed, Ennas Abdul Hussein, Saadiyah A. Dhahir

Baghdad Science Journal

In this work, a sample of mesoporous silica (MPS) with a high surface area was prepared by hydrothermal synthesis procedure. To investigate the properties of the prepared sample, it was characterized using different techniques like N₂ Adsorption-desorption, Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Atomic Force microscopy (AFM). In order to evaluate the efficiency of the prepared MPS, adsorption study was carried to examine the ability of MPS to remove crystal violet dye (CV) from aqueous solution. The study was done in different conditions of initial concentration (25-60 mg/L) of dye, dosage of MPS (0.01-0.05 g) and temperature …

Genetic Analysis Of Spp1 Polymorphism In Samples Of Iraqi Colon Cancer Patients, Ghufran Kadhim Talal, Ahmed Younus Abed

Baghdad Science Journal

Cancer is a multistage defect and very complex disease. It is distinguished by particular genetic mutations that lead to a loss of management of cellular functions during uncontrolled growth of normal cells leading to malignant cells. CA18 occurs by the abnormal growth of cells in colon. SPP1 is multifunctional protein. The aim of this study is to study genetic polymorphism of SPP1gene in CA18 patients along with healthy individuals to find the relationship with CA18 and compare both globally. DNA was extracted and the genetic polymorphism of the SPP1gene was determined. Genetic analysis of SPP1 (in rs11730582 T/C region) polymorphism …

Ability Of Staphylococcus Epidermidis And Escherichia Coli To Eliminate Cadmium, Lead And Copper From Tigris River In Mosul City, May A. Abdul Kader, Ghada A. Mohammad

Baghdad Science Journal

The heavy metals are non-biodegradable and could be toxic and carcinogenic, thus, their improper concentration in water could result in critical health issues for living organisms. Therefore, this study aimed to remove three heavy metals (Cadmium, lead and copper) by biological treatment especially using environmental nonpathogenic bacteria such as Staphylococcus epidermidis and Escherichia coli. Eight samples were collected from Tigris River from different regions of Mosul city. Two containers were used for each sample to measure the concentration of minerals before and after treatment by bacteria. The results showed that E. coli was more efficient in removing two heavy …

Msw Portfolio, Julia Marie Sweeney

Graduate Student Portfolios, Professional Papers, and Capstone Projects

This portfolio reflects my personal and professional growth throughout the University of Montana’s MSW program. Grounded in lived experience, child advocacy work, and academic learning, it highlights my development across core competencies and advanced integrated practice.

Chromatographic Developments For The Analysis Of Toxic Organic Chemicals In The Environment And Food Samples, Stephanie Devi Nauth

Graduate Thesis and Dissertation post-2024

As awareness grows about compounds harmful to human health, the need for sensitive detection methods at low concentration levels becomes increasingly critical. Chromatography is a powerful analytical technique that enables the separation of complex mixtures, allowing for the simultaneous analysis of multiple analytes—even at trace levels. This dissertation applies chromatography in two distinct contexts: (1) Investigating the sorption of polycyclic aromatic sulfur heterocycles (PASHs) onto nylon microplastics; and (2) Detecting illegal dyes in various food matrices.

Microplastics have emerged as significant vectors for transporting pollutants into aquatic ecosystems. Due to their hydrophobic nature, many organic pollutants readily sorb onto microplastics …

Isolation And Divergence Of Greater Earless Lizards (Phrynosomatidae: Cophosaurus) In Western North America Support Multiple Diversification Processes And A Complex Demographic History, Christopher Robert Blair, Carlos Joaquín Pavón-Vázquez, Qaantah Rana, Keaka Farleigh, Erika Crispo, Thomas Panzera, Felipe De Medeiros Magalhães, Britt White, Uri Omar Garcia-Vazquez

Publications and Research

The Southwestern US and northern Mexico continue to serve as ideal regions to elucidate the suite of ecological and evolutionary processes influencing lineage diversification. The complex geological history of arid North America, coupled with paleoclimate change during the Pleistocene and diverse ecoregions makes a multipronged approach to hypothesis testing necessary. We combine thousands of loci from a genotyping-by-sequencing (GBS) approach along with mitochondrial DNA (mtDNA) sequences from the Greater Earless Lizard (Cophosaurus texanus) to examine diversity and test for cryptic population structure. We also apply recently developed coalescent approaches in a Bayesian framework to estimate migration rates. Population …

Lipidomic And Proteomic Insights From Extracellular Vesicles In The Postmortem Dorsolateral Prefrontal Cortex Reveal Substance Use Disorder-Induced Brain Changes, Chioma M Okeoma, Wasifa Naushad, Bryson C Okeoma, Carlos Gartner, Yulica Santos-Ortega, Calvin Vary, Savio Lima-Bastos, Victor Corasolla Carregari, Martin R Larsen, Alessio Noghero, Consuelo Walss-Bass, Rodrigo Grassi-Oliveira

Faculty, Staff and Student Publications

Substance use disorder (SUD) significantly increases the risk of neurotoxicity, inflammation, oxidative stress, and impaired neuroplasticity. The activation of inflammatory pathways by substances may lead to reactive astrogliosis and chronic neuroinflammation, potentially mediated by the release of extracellular particles (EPs), such as extracellular condensates (ECs) and extracellular vesicles (EVs). These particles, which reflect the physiological, pathophysiological, and metabolic states of their cells of origin, might carry molecular signatures indicative of SUD. In particular, our study investigated neuroinflammatory signatures in SUD patients by isolating EVs from the dorsolateral prefrontal cortex (dlPFC) Brodmann's area 9 (BA9) from postmortem subjects. We isolated BA9-derived …

Mathematical Modeling And Association Analysis Decipher The Impact Of The Gut Microbiome On Cancer Immunotherapy, Andreas G Hadjigeorgiou, Constantinos Harkos, Aditya K Mishra, Golnaz Morad, Sarah B Johnson, Nadim J Ajami, Jennifer A Wargo, Lance L Munn, Triantafyllos Stylianopoulos, Rakesh K Jain

Faculty, Staff and Student Publications

The gut microbiome has emerged as a key regulator of response to cancer immunotherapy. However, a better understanding of the underlying mechanisms by which the microbiome influences immunotherapy is needed to identify strategies to optimize outcomes. To this end, we developed a mathematical model to obtain insights into the effect of the microbiome on the immune system and immunotherapy response. This model was based on (i) gut microbiome data derived from preclinical studies, (ii) mathematical modeling of the antitumor immune response, (iii) association analysis of microbiome profiles with model-predicted immune profiles, and (iv) statistical models that correlate model parameters with …