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Danicamtiv Reduces Myosin's Working Stroke But Activates The Thin Filament By Accelerating Actomyosin Attachment, Brent Scott, Lina Greenberg, Caterina Squarci, Kenneth S Campbell, Michael J Greenberg

2020-Current year OA Pubs

Heart failure is a leading cause of death worldwide, and even with current treatments, the 5-y transplant-free survival rate is only ~50 to 70%. As such, there is a need to develop new treatments for patients that improve survival and quality of life. Recently, there have been efforts to develop small molecules for heart failure that directly target components of the sarcomere, including cardiac myosin. Danicamtiv is one of these molecules; however, its direct effects on myosin's single molecule mechanics and kinetics are not well understood. Using optical trapping techniques, stopped flow transient kinetics, and in vitro reconstitution assays, we …

Data For Recreation Effects On Soil Carbon, Biocrust, Soil Stability, And Invasives In Indian Creek, Bears Ears National Monument, Elisabeth M. Currit, Tara B. Bishop, April Hulet, Nathan Allen Currit, Teresa Gomez, Nathan T. Thompson, Shannon Lambson, Heidi Jean Wilding Mann, Evan Miller, Erin Jones, Alexandra Olson, Katherine A. Anselmi, Meredith Murdock, Ella Reid, Kirsten Steele, Indy Lawrence, Neil Hansen

ScholarsArchive Data

This data is about how different types of recreation impact different soil and vegetation properties related to soil carbon in Indian Creek, Bears Ears National Monument. The data was collected from May 2023 to October 2023. It contains plant functional group cover, biocrust cover and type, soil stability, and compaction data for each individual site. There are 5-7 of each site type: camping, foot trails, and motorized offroading trails, and undisturbed sites, with a total of 30 sites. It also contains the code used to analyze the raw data and the figures created.

Danicamtiv Reduces Myosin’S Working Stroke But Activates The Thin Filament By Accelerating Actomyosin Attachment, Brent Scott, Lina Greenberg, Caterina Squarci, Kenneth S. Campbell, Michael J. Greenberg

Physiology Faculty Publications

Heart failure is a leading cause of death worldwide, and even with current treatments, the 5-y transplant-free survival rate is only ~50 to 70%. As such, there is a need to develop new treatments for patients that improve survival and quality of life. Recently, there have been efforts to develop small molecules for heart failure that directly target components of the sarcomere, including cardiac myosin. Danicamtiv is one of these molecules; however, its direct effects on myosin’s single molecule mechanics and kinetics are not well understood. Using optical trapping techniques, stopped flow transient kinetics, and in vitro reconstitution assays, we …

Ifit3 Activation Significantly Contributes To Hiv-1-Associated Neurodegenerative Disorder-Mediated Neuroinflammation, Ranjit Kumar Das, Nirakar Sahoo, Deepa Roy, Chun Xu, Jose Galarza, Maria Camila Mejia Garza, Hansapani Rodrigo, Asim K. Duttaroy, Upal Roy

Health & Biomedical Sciences Faculty Publications

Introduction: The advent of effective combination antiretroviral therapy (cART) has significantly improved HIV-1 treatment, saving millions of lives. However, HAND remains a concern, particularly among aging individuals with HIV-1. The mechanisms underlying HAND are not well understood.

Methods: This study investigated the role of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and its upstream regulator, signal transducer, and activator of transcription 1 (STAT1), in HAND pathology. Using the SH-SY5Y neuroblastoma cell line and HIV-infected humanized mice, we examined the effects of the cART drugs, HIV Tat protein, and HIV-1 virus on STAT1 and IFIT3 expression.

Results: The results showed that …

Dissection Of Surface Area-Dependent Lipolysis At A Single Organelle Level, Chengxin Ma, Yan Liang, Xinmeng Che, Yuqi Li, Jin Wu, Li Xu, Hongyuan Yang, Tong-Jin Zhao, Feng-Jung Chen, Peng Li

Faculty, Staff and Student Publications

Lipid droplets are dynamic organelles whose size and number signify their role in energy. However, owing to cellular heterogeneity and technological limitations, the relationship between the lipolytic ability and lipid droplet morphology is unclear. Here, we developed a live-cell imaging assay using geometric analysis to quantify cellular lipolysis at a single organelle level, designated imaging lipolysis. Using imaging lipolysis and super-resolution imaging, we found that lipolysis is controlled by both lipase-accessible lipid droplet surface area and lipase activity. Moreover, lipid droplet fusion regulatory proteins CLSTN3β/CIDEs promote lipolysis by increasing the total lipid droplet surface area-to-volume ratio in biophysical regulation. We …

Suppression Of Cardiac Allograft Vasculopathy By A Macrophage Efferocytosis Receptor, Sahil Shah, Aparnaa Ananthakrishnan, Matthew Deberge, Kristofor Glinton, Rebecca T L Jones, Mallory Filipp, Ivana Shen, Joey Lockhart, Connor W Lantz, Edward B Thorp

Faculty, Staff and Student Publications

Cardiac allograft vasculopathy remains a major cause of late morbidity following heart transplantation. While accumulating evidence implicates innate macrophages in the inflammatory progression of CAV, the underlying mechanisms remain incompletely understood. In murine models of CAV, we identified proteolytic cleavage of MERTK, a key anti-inflammatory receptor on macrophages, as a contributing factor to CAV progression. In a model of CAV, MERTK deficiency accelerated allograft rejection and increased intimal leukocyte infiltration. In contrast, mice expressing a genetically cleavage-resistant form of MERTK (MertkCR/CR) exhibited prolonged graft survival, reduced intimal thickening, diminished immune cell infiltration, and decreased circulating effector T cells. …

The Role Of Recruited Adipose Stromal Cells And Their Fibroblastic Differentiation In Cancer, Lingyi Cai, Mikhail G Kolonin, Dimitris Anastassiou

Faculty, Staff and Student Publications

Adipose stromal cells (ASCs) are perivascular mesenchymal progenitors of adipose tissue. In cancer patients, ASCs can mobilize and migrate to the tumor, where they subsequently play an important role in cancer progression. This biological process involves the conversion of recruited ASCs into cancer-associated fibroblasts (CAFs). ASC-derived CAFs influence the tumor microenvironment through extracellular matrix remodeling, vascularization, and immunomodulation. These and other processes mediated by secreted paracrine factors also affect gene expression in carcinoma cells to promote the epithelial-mesenchymal transition (EMT), metabolic adaptation, survival, and invasiveness of cancer cells. ASC-derived CAFs can enhance tumor aggressiveness, accounting in part for the link …

The Problem Of Market Power In Postwar America: Antitrust Law, Regulatory Discourse, And Changing Ideas Of Market Power, Laura Phillips-Sawyer

Scholarly Works

US antitrust law prohibits anticompetitive restraints of trade, including collusion among firms and single-firm monopolization. The law serves as a democratic check against concentrations of private economic power. However, in the post-World War II era, the law has changed largely through case law, rather than through statutory interventions. Thus, understanding the role that antitrust law has played in reconciling capitalism and democracy requires us to ask what has influenced changes to antitrust law. This chapter traces antitrust law’s postwar development across three key periods: 1940–1970s, mid-1970s to 2010s, and 2010s to present. It argues that each era’s approach to the …

Artificial Intelligence In Ocular Transcriptomics: Applications Of Unsupervised And Supervised Learning, Catherine Lalman, Yimin Yang, Janice L. Walker

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Transcriptomic profiling is a powerful tool for dissecting the cellular and molecular complexity of ocular tissues, providing insights into retinal development, corneal disease, macular degeneration, and glaucoma. With the expansion of microarray, bulk RNA sequencing (RNA-seq), and single-cell RNA-seq technologies, artificial intelligence (AI) has emerged as a key strategy for analyzing high-dimensional gene expression data. This review synthesizes AI-enabled transcriptomic studies in ophthalmology from 2019 to 2025, highlighting how supervised and unsupervised machine learning (ML) methods have advanced biomarker discovery, cell type classification, and eye development and ocular disease modeling. Here, we discuss unsupervised techniques, such as principal component analysis …

Pfkfb3 Activates Cad To Enhance De Novo Pyrimidine Synthesis For Cell Growth, Qingen Da, Yongfeng Cai, Qian Ma, Qiuhua Yang, Yapeng Cao, Yaqi Zhou, Dingwei Zhao, Zhiping Liu, Jiean Xu, Junming Quan, Liang Zhang, Rui Wang, Xuejun Jiang, Xiao Liu, Kunfu Ouyang, Zhen Han, Jikui Liu, Tao Wang, Chunxiang Zhang, Neal L Weintraub, David J R Fulton, Jun Zhao, Mei Hong, Zigang Li, Yuqing Huo

Faculty, Staff and Students Publications

Aerobic glycolysis, termed the Warburg effect, is one of the aberrant metabolic pathways in highly proliferating cells. Glycolysis provides glycolytic metabolites to support the generation of biomass, such as nucleotides, amino acids, and lipids. Research on the direct interactions between glycolysis and other metabolic pathways is an emerging field that has garnered significant interest. Phosphofructokinase-2/fructose-2,6-bisphosphatase 3 (PFKFB3) activates glycolysis by synthesizing fructose-2,6-bisphosphate (F2,6BP), which allosterically activates the rate-limiting enzyme 6-phosphofructo-1-kinase (PFK-1). In this study, we found that PFKFB3 directly interacts with and regulates the phosphorylation of carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), the enzyme catalyzing the first three …

High-Speed Voltage Imaging Of Action Potentials In Molecular Layer Interneurons Reveals Sensory-Driven Synchrony That Augments Movement, Spencer T Brown, Meghana R Holla, Michelle A Land, Shuyuan Yang, Alex J Mcdonald, François St-Pierre, Indira M Raman

Faculty, Staff and Students Publications

Testing whether the synchrony of action potential firing is a cerebellar coding mechanism requires simultaneous recording, with high temporal fidelity, from populations of identified neurons. Here, we used targeted one-photon voltage imaging at 2–4 kHz to record action potentials from groups of ~10–300 molecular layer interneurons (MLIs) expressing a positively tuned, genetically encoded voltage indicator, FORCE1f or pAce. In awake resting mice, crus I MLIs fired brief (~1-ms) spikes at 20–60 spikes/s. Sensory stimuli of air puffs to the whiskers evoked short-latency (< 10 ms) increases in spiking probability. In most trials, >50% of MLIs fired synchronously with 4-ms temporal precision. The magnitude of puff-evoked whisks correlated tightly with …

Cathartocytosis: Jettisoning Of Cellular Material During Reprogramming Of Differentiated Cells, Jeffrey W Brown, Xiaobo Lin, Gabriel Anthony Nicolazzi, Xuemei Liu, Thanh Nguyen, Megan D Radyk, Joseph Burclaff, Jason C Mills

Faculty, Staff and Students Publications

Injury causes differentiated cells to undergo massive reprogramming to become proliferative and repair tissue via paligenosis. Gastric chief cells use paligenosis to reprogram into progenitor-like spasmolytic-polypeptide-expressing metaplasia (SPEM) cells. Stage 1 of paligenosis is the downscaling of mature cell architecture via a process involving lysosomes. Here, we notice that sulfated glycoproteins are not only digested during paligenosis but also excreted into the gland. Various genetic and pharmacological approaches show that endoplasmic reticulum membranes and secretory granule cargo are also excreted and that the process proceeds in parallel with but is mechanistically independent of autophagy. Three-dimensional light and electron microscopy demonstrated …

Multiplex Imaging Mass Cytometry Reveals Prognostic Immunosuppressive Subpopulations And Macrophage-Driven Metastasis In Osteosarcoma, Benjamin B Gyau, Junyan Wang, Weiguo Wu, Brooks Scull, Angela M Major, Weidong Jin, Justin M M Cates, John Hicks, Tsz-Kwong Man

Faculty, Staff and Students Publications

Osteosarcoma is a type of bone cancer that mostly affects kids and teens. The biggest danger is when the cancer spreads to the lungs, and current treatments do not work well for those cases. Immunotherapy, which helps the body’s immune system fight cancer, has worked for other cancers but is limited for osteosarcoma because we do not fully understand the environment around the tumor. Our research looked at various cells around the tumor to study why patients have worse outcomes. We found that certain cells, called M2 macrophages (M2) and myeloid-derived suppressor cells (MDSC), which weaken the immune system, are …

Choice Of Animal Models To Investigate Cell Migration And Invasion In Glioblastoma., Piyanka Hettiarachchi, Taeju Park

Manuscripts, Articles, Book Chapters and Other Papers

Glioblastoma is an aggressive and prevalent form of brain cancer characterized by rapid tumor cell migration and invasion into surrounding healthy tissues, making it resistant to conventional treatments. Despite advances in therapeutic approaches, patient prognosis remains poor, with a median survival of approximately 15 months. Tumor cell infiltration along perivascular spaces and white matter tracts is a major driver of recurrence, underscoring the need for experimental models that accurately capture these invasive behaviors. Animal models remain indispensable for this purpose, offering insights that cannot be fully replicated in vitro. This review focuses on applying animal models to elucidate the mechanisms …

The Monstrous Other: Exploring Negative Representations Of Queerness In Genre Cinema, Mark Gambino

Imaginings: creative practice and inquiry

The following literature review is an extract from my SAE University College Masters thesis, Disrupting negative representations of queerness in genre cinema, conferred in September 2024. The project explored representations of the queer Other in film horror, through artefact-exegesis, with the creative artefact, horror film Shadows, interrogating the coding of trans / queer characters as the ‘monstrous Other’ in the horror film genre, and how this notion can be subverted and transcended.

Impact Of Accounting Information Systems And Islamic Organizational Culture On Information Quality, Oelma Arfannur Azizi, Busra Busra, Marjulin Marjulin

Arab Economic and Business Journal

Amid growing demands for transparency and accuracy in financial management, an effective Accounting Information System plays a crucial role in ensuring that financial data is accurately. However, the implementation of such technology must be supported by a strong organizational culture,. The method employed in this research is a quantitative approach, utilizing inferential statistics to explain the impact of various research variables. The statistical tool used is Structural Equation Modeling -. The results of this study indicate that both the Accounting Information System and Islamic Organizational Culture have a positive and significant impact on the quality of accounting information

Fostering Wellness In Nursing: Insights From Mental Health Bulletin Boards, Christina Marie Lightner, Kelly A. Wolgast, Khalid Almasloukh, Amy Criscitello

Creating Healthy Work Environments (CHWE)

The College of Nursing promotes a wellness culture to address growing mental health challenges among nursing students and professionals (Lipson et al., 2022). Grounded in the 12 dimensions of wellness (Gawlik et al., 2024), these initiatives foster resilience, support healthy lifestyle behaviors, and create a nurturing environment for the nursing community. Mental health and wellness interventions play a crucial role in improving the well-being of college students, including nursing students (Melnyk et al., 2020).
This presentation outlines the development and thematic analysis of Mental Health Bulletin Boards (MHBBs), designed to engage nursing students and professionals in self-care and resilience-building practices. …

Proceedings Of The 2025 Berry Summer Thesis Institute, University Of Dayton. University Honors Program, Dorian Borbonus, Nicholas J. Canfield, Triet M. Cao, Thomas Crossman, Jeffrey Huettemann, Aung Myat Khaung, Chase Kinder, Elizabeth Krahe, Anh Duc Nguyen, Quynh Anh Nguyen, Anna M. Petre, Kayden G. Rothery, Anna M. Schmitz, Rachel Zeller

Proceedings of the Berry Summer Thesis Institute (2013-Present)

Thanks to a gift from the Berry Family Foundation and the Berry family, the University Honors Program launched the Berry Summer Thesis Institute in 2012. The institute introduces students in the University Honors Program to intensive research, scholarship opportunities and professional development. Each student pursues a 12-week summer thesis research project under the guidance of a UD faculty mentor. This contains the product of the students' research.

Rotunda - Vol 105, No 1 - Aug 25, 2025, Longwood University

Rotunda

No abstract provided.

Strategies Used In The Biotechnology Industry To Develop And Continuously Improve Compliant Regulatory Processes To Meet The Food And Drug Administration Quality Federal Regulations, Vicki Mckee

Walden Dissertations and Doctoral Studies

Failure to comply with the U.S. Food and Drug Administration (FDA) quality regulations can lead to enforcement actions for the biotechnology (biotech) industry. Noncompliant regulatory strategies have the potential for adverse medical outcomes for patients. As a result, managers in the biotech industry are concerned about ensuring compliance to avoid adverse medical outcomes for patients and sustain business operations. Grounded in Deming’s total quality management concept, the purpose of this qualitative pragmatic inquiry project was to explore effective quality management strategies that managers in the biotech industry use to develop and continuously improve compliant regulatoryQuality Federal Regulations processes to meet …

University Of New Hampshire 2025-2026 Faculty Senate Xxx - August 25, 2025 Minutes Summary, Faculty Senate

Faculty Senate Agendas & Minutes

No abstract provided.

The Gift Of Giving: Recognizing Donors And Revealing Donation Amounts, K. Pun Winichakul

Economics: Faculty Publications

Publicly announcing how much individuals donate on behalf of themselves is a common fundraising strategy. For tribute gifts made on behalf of others, however, charities only reveal donor identities to the honoree with few revealing the size of their contributions. This paper examines the fundraising consequences of recognizing donors with and without information about donation amounts when notifying honorees of gifts made on their behalf. I find that revealing contribution amounts in addition to recognizing donors benefits fundraisers. I find that both the likelihood of giving on behalf of others and contribution amounts increase when honorees learn how much donors …

Lanthorn, Vol. 60, No. 02, August 25, 2025, Grand Valley State University

Volume 60, August 4, 2025 – current

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.

Are Orthopaedic Trauma Surgeons At Risk Of Occupational Noise-Induced Hearing Loss?, Vincent W. Lau, Stephanie A. Kwan, Jack Graham, Gregory K. Deirmengian

Rothman Institute Papers

OBJECTIVES: Orthopaedic surgeons are at risk of occupational, noise-induced hearing loss due to exposure to instruments in the operating room. The primary objective of this study was to determine whether orthopaedic trauma procedures exceed recommended decibel (dB) limits. The secondary objective was to demonstrate which actions during a case create the highest sound levels.

METHODS: Intraoperative recordings were taken during orthopaedic trauma surgeries and classified into open reduction internal fixation (ORIF), intramedullary nailing, closed reduction percutaneous pinning, external fixation, soft tissue procedures, or a combination thereof. Recordings were taken of drilling, screw insertion, suctioning, saw use, and mallet striking. Decibel …

Development Of Novel Small Molecules As Potential Anti Cancer Agents, Robert John Chitren

Theses and Dissertations

This doctoral thesis explores the discovery and characterization of two novel small molecules, designated as KS100 and (R,S’)-MNF, with potential anti-cancer properties. The study encompasses a comprehensive investigation into the molecular mechanisms underlying the anti-cancer effects of these compounds, as well as their therapeutic potential in preclinical models. These findings highlight the potential of KS100 and (R,S’)-MNF as lead candidates for cancer therapy, paving the way for further preclinical development and clinical translation.

The Free Press Vol. 57, Issue 1, 08-25-2025, Julz Levesque, Caraline Squires

Free Press, The

Welcome Letter from the Dean--Learning Commons Welcome Letter--SEAB Welcome--University Health & Counseling--Public Safety & Journal of Scandal--Welcome from Editors=in=Chief

The Echo: August 25, 2025, Taylor University

2025-2026 (Volume 113)

No abstract provided.

Advancing Institutional Sustainability Through Green Roofs: Performance-Based Policy For Rit, Theoren Heilman

Theses

Utilization of green roof systems continues growth as a sustainable architectural strategy, offering enhancements to key building performance metrics while contributing to overall community resilience. The ability of green roofs to deliver a wide range of performance benefits, even in environments with harsh climatic conditions, positions them as an asset for advancing sustainable development. Within this context, university campuses represent an underutilized, yet important, setting for green roof adoption. Functioning much like small cities, campuses contain diverse building types and extensive infrastructure networks, which draw significant energy demands, positioning them to lead in sustainable building practices. Yet, most lack rigorous, …

Feasibility Of Osteopathic Manipulative Treatment For Low Back Pain In Rural Chimaltenango, Guatemala: A Pilot Study, Monica Aspra Rubi, Ambrose Loc T. Ngo, Grace Cooper, Jared Nichols

Student Publications

Background

Low back pain (LBP) is a global health issue that primarily affects underserved communities with limited access to healthcare. Osteopathic manipulative treatment (OMT) offers non-pharmacological, hands-on interventions that align with its core principle of holistic care. However, little is known about the feasibility and perception of OMT in rural Chimaltenango, Guatemala. This pilot study assesses the feasibility, effectiveness, and participant perception of osteopathic principles in managing LBP complaints.

Methods

Adult participants experiencing LBP were recruited by the research team during Kansas City University College of Osteopathic Medicine’s (KCUCOM) global health mission trip, following their one-day primary care visit. Pre- …

Is Poverty The Root Of All Crime? Rethinking Criminality Amid Institutional Failure In Madagascar, Ramalina Ranaivo Mikea Manitra

Jurnal Kriminologi Indonesia

Madagascar continues to grapple with high levels of poverty and weak institutional capacity, creating conditions conducive to widespread and persistent criminality. This paper aims to examine how structural poverty, institutional failure, and state fragility interact to shape patterns of crime through the lens of criminological theories such as strain theory, social disorganisation, and state-centred criminology. Using a qualitative methodology based on a comprehensive literature review of empirical studies, policy documents, and international reports, the paper explores the socio-economic and institutional dynamics underpinning crime in Madagascar. The findings reveal that poverty-driven crime often arises as a rational response to unmet needs, …