Digital Commons Network^™

The 9th Annual Lafora Science Symposium: A Rare Epilepsy Community Makes Progress Towards Clinical Readiness, Meredith I. Williams, Katherine J. Donahue, Pascual Sanz, Souad Messahel, Jose M. Serratosa, Jordi Duran, Roberto Michelucci, Lorenzo Muccioli, Antonio Delgado-Escueta, Viet-Hong Nguyen, Berge A. Minassian, Matthew S. Gentry

Pharmacy Faculty Articles and Research

Lafora disease (LD) is a fatal childhood progressive myoclonus epilepsy and glycogen storage disease that is caused by recessive mutations in either EPM2A or EPM2B. The hallmarks of LD are cytoplasmic, aberrant glycogen-like aggregates, called Lafora bodies (LBs), that drive disease progression. The 9th Annual Lafora Science Symposium was held in San Diego, California and brought together over 70 researchers, clinicians, academic trainees, and friends and family members of patients with LD and 80 attendees joined virtually. This symposium focused primarily on international collaborations for therapeutic development and biomarker identification and strategies for preparing the Lafora community for upcoming …

Heterozygous Variants In Plcg1 Affect Hearing, Vision, Cardiac, And Immune Function, Mengqi Ma, Yiming Zheng, Mingxi Deng, Shenzhao Lu, Xueyang Pan, Xi Luo, Michelle Etoundi, David Li-Kroeger, Kim C Worley, Lindsay C Burrage, Lauren S Blieden, Aimee Allworth, Wei-Liang Chen, Giuseppe Merla, Barbara Mandriani, Catherine E Otten, Pierre Blanc, Jill A Rosenfeld, Debdeep Dutta, Shinya Yamamoto, Michael F Wangler, Ian A Glass, Jingheng Chen, Elizabeth Blue, Paolo Prontera, Jeremie Rosain, Sandrine Marlin, Seema R Lalani, Hugo J Bellen, Undiagnosed Diseases Network

Duncan NRI Faculty and Staff Publications

Phospholipase C isozymes (PLCs) hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP₂) into inositol 1,4,5-trisphosphate (IP₃) and diacylglycerol (DAG), important signaling molecules involved in many cellular processes including Ca²⁺ release from the endoplasmic reticulum (ER). PLCG1 encodes the PLCγ1 isozyme that is broadly expressed. Hyperactive somatic mutations of PLCG1 are observed in multiple cancers, but only one germline variant has been reported. Here, we describe seven individuals with heterozygous missense variants in PLCG1 [p.(Asp1019Gly), p.(His380Arg), p.(Asp1165Gly), and p.(Leu597Phe)] who present with hearing impairment (5/7), ocular pathology (4/7), cardiac septal defects (3/6), and various immunological issues (5/7). To model these …

Topological Indices And Quantitative Structure-Property Analysis Of Novel Drugs For Acute Lymphoblastic Leukemia Treatment, P. Nathiya, M. Suresh, Jalal Hatem Hussein Bayati

Baghdad Science Journal

Acute lymphoblastic leukemia in children is a bone marrow and blood cancer. Healthy blood cells begin to change and grow out of control when leukemia initially occurs. Acute lymphoblastic leukemia (ALL) is an immune system-related malignancy of the lymphocytes, a subtype of white blood cells. The most common form of cancer affecting children is ALL. In this work, the topological indices are derived from the structure of a chemical graph, and predict the physical, chemical, and biological characteristics of several anticancer drugs. ALL are usually treated with drugs such as methotrexate, 6-mercaptopurine (6-MP), vincristine, L-asparaginase, and/or prednisone, etc., which are …

A Clustering Technique Based On The Hard K-Means (H.Km.) Method To Determine The Governorate That Have More Influence For Spreading Covid-19 In The Kingdom Of Saudi Arabia, Rand Muhaned Fawzi, Wurood R. Abd Al-Hussein, Iden Hassan Alkanani

Baghdad Science Journal

The Kingdom of Saudi Arabia is the gathering place of most of the nationalities of the Islamic world. When a certain disease spreads, it will be important to know which governorate has the greatest influence on the spread of the disease and take the necessary precautions to limit its spread, and this is the goal of this study. COVID-19, The SRS-COV-2 coronavirus that caused the most recent pandemic is known as the Corona pandemic. To determine which Saudi governorates had the greatest influence on the epidemics spread, data was gathered for thirteen governorates over two months (July and August). The …

A New Approach For Selecting Optimal Neuron Number In Input Layer Of Generalized Regression Neural Network Model, Muhammad Muhajir, Hermansah Hermansah, Aditya Pandu Wicaksono, Lathifah Aliya Pratiwi

Baghdad Science Journal

This study aimed to determine the input layer neurons for the Generalized Regression Neural Network (GRNN) model by using various classical methods, namely 1) Partial Autocorrelation Function (PACF), 2) frequency-based method, 3) frequency and Forward Selection (FS), 4) frequency and Backward Elimination (BE), 5) frequency and step-based methods, and 6) frequency method combined with the Least Absolute Shrinkage and Selection Operator (LASSO). These classical methods were combined with various parameters within GRNN, including smoothing parameters, forecasting strategies, and transformations. The most accurate model, with the lowest RMSE, MAE, MAPE, and SMAPE values, resulted from the combination of frequency and BE, …

A New Efficient Method To Solve Bi-Objective Transportation Problems Under Fuzzy Parameters, Ramakant Sharma, Sohan Lal Tyagi

Baghdad Science Journal

The transportation problem (TP) is a classical optimization problem in operations research and logistics. Due to several factors, real-life situations may have inconsistent supply, demand, and unit transportation costs. The fuzzy numbers represent these inaccurate data. In the present scenario, the decision-maker handles several objectives simultaneously. This paper presents a simple method to solve linear Bi-Objective Transportation Problems (BOTP) using (η, ξ) interval-valued fuzzy numbers (IVFN), offering a more realistic way to model uncertainty than normal fuzzy numbers. Using the signed distance ranking, the Fuzzy BOTP was transformed into the equivalent crisp BOTP. In this paper, A method is developed …

Emerging Challenges In Adversarial Deep Learning For Computer Vision And Cybersecurity, Younis Al-Arbo, Asmaa Alqassab

Baghdad Science Journal

The emergence of "Deep Learning" DL has revolutionized the scope of cybersecurity and computer vision. However, this technology is not immune to emerging challenges that can affect its performance and security. One major challenge is the availability of large datasets for training DL algorithms. Furthermore, there is a need for improved algorithms and architectures that can effectively process such datasets. Another challenge is the constant evolution of cyber threats, which require the development of new DL models to defend against them. Additionally, the interpretability and explain ability of DL models in cybersecurity pose a significant challenge, as their black-box nature …

A Comparative Of Detecting Physical And Cyber Attacks On Drones Using Machine Learning And Deep Learning Techniques, Khattab M Ali Alheeti, Sara Abbas Rafa, Maha Mahmood, Aythem Khairi Kareem, Mohammad Aljanabi, Ahmed Adil Nafea

Baghdad Science Journal

Multitudes of Unmanned Aerial Vehicles (UAVs) are generally embraced in military and civilian applications. Yet, this physical-cyber method is intimidated by cyber-attacks. Recently, Machine Learning (ML) based attacks detection approaches have been effectively embraced to detect cyber-attacks. This paper presented the Intrusion Detection Security (IDS) approach. The proposed approach investigates UAVs' cyber and physical attributes under normal process and attack circumstances. Two types of cyber-attacks have been classified: Denial-of-service (DoS) and replay. This study developed IDS approaches established on ML and Deep Learning (DL) prototypes, including Support Vector Machine (SVM), K-Nearest Neighbor (KNN), Naive Bayes (NB), and One Dimensional Convolutional …

An Efficient Glaucoma Classification For Fundus Images By Pre-Trained Deep Cnns And Accuracy-Weighted Voting Classifier Fusion, Salam Jabbar Edan, Mohammad-Reza Feizi-Derakhshi

Baghdad Science Journal

Glaucoma, a chronic eye disease characterized by progressive optic nerve damage, remains a significant public health concern worldwide. Early and accurate diagnosis is crucial for timely intervention and preventing irreversible vision loss. In this paper, developed an approach for glaucoma classification utilizing pre-trained convolutional neural networks (CNNs), specifically ResNet-101, ResNet-18, and DarkNet-19. Also employed accuracy-based weighted voting as a classifier fusion technique. Leveraging the capabilities of these CNN architectures, extract high-level features from retinal fundus images and apply transfer learning to adapt the networks for glaucoma classification. Leveraging the capabilities of these CNN architectures, extract high-level features from retinal fundus …

Accurate Skin Lesion Segmentation Through Feature Fusion In Dermoscopy Images, Fallah H. Najjar, Farhan Mohamed, Asniyani Nur Haidar Abdullah, Mohd Shafry Mohd Rahim, Karrar A. Kadhim

Baghdad Science Journal

In the diagnosis of skin cancer, dermoscopy images have to be evaluated for feature extraction. In this paper, the authors design a new method to enhance the skin dermoscopy image segmentation accuracy by eliminating artifacts and hair using photometric quasi-invariants, followed by a unique approach for skin lesion segmentation using histogram-based feature fusion. Histogram features are used to extract data range and mean images from the histogram, and these are fused with the free artifacts and hair image to generate the final image. the authors used the PH2 dataset for the proposed segmentation method because it contains the ground truth …

Nf1-Depleted Er+ Breast Cancers Are Differentially Sensitive To Cdk4/6 Inhibitors, Ze-Yi Zheng, Anran Chen, Eric J Jaehnig, Meenakshi Anurag, Jonathan T Lei, Long Feng, Chenwei Wang, Diana Fandino, Purba Singh, Hilda Kennedy, Ghazal Yadav, Craig T Vollert, Jill Tsai, Xi Chen, Yi Li, Bora Lim, Alastair Thompson, Shunqiang Li, Charles E Foulds, Bing Zhang, Matthew J Ellis, Eric C Chang

Faculty, Staff and Students Publications

Neurofibromin/NF1 is a RAS (rat sarcoma virus) GTPase activating protein and estrogen receptor (ER) transcriptional corepressor. NF1low status, identified by copy number loss or low mRNA/protein expression is associated with endocrine therapy resistance in approximately 20% of ER+/HER2− (human epidermal growth factor receptor 2) early-stage breast cancers. The identification of targeted treatments for NF1low ER+/HER2− breast cancer is therefore a priority. In this study proteogenomic analysis of ER+/HER2− breast cancer demonstrated that NF1low tumors exhibited elevated cyclin-dependent kinase 4/6 (CDK4/6) activity. In cell lines, NF1-deletion had a dual effect on CDK4 activity. First, by promoting ER recruitment to CCND1 …

A Protective Role Of Src-1 Against Aging Associated Cognitive Decline, Hesong Liu, Yongjie Yang, Jonathan C Bean, Yang He, Hailan Liu, Rambabu Majji, Chen Liang, Nan Zhang, Meng Yu, Longlong Tu, Qingzhuo Liu, Yue Deng, Kristine M Conde, Na Yin, Mengjie Wang, Yongxiang Li, Junying Han, Sanika Vattakuzhiyil Jossy, Megan Elyse Burt, Hari Krishna Yalamanchili, Chunmei Wang

Duncan NRI Faculty and Staff Publications

Introduction: Research indicates a strong correlation between obesity and the risk of dementia, both are linked to steroid receptor coactivator-1 (SRC-1), a transcriptional coactivator.

Methods: We used RNA sequencing analysis (RNA-Seq) to investigate the transcriptome of SRC-1-KO mice, and identified S100 calcium-binding protein A6 (S100A6), an AD associated gene, as one target of SRC-1. We tested cognitive behaviors in SRC-1-KO mice and mice with a humanized SRC-1 mutation (SRC-1L1376P), and performed promoter luciferase assays on S100A6.

Results: Loss of SRC-1 caused alterations in gene signatures that are commonly associated with neurodegenerative diseases, including AD, and diminished the neural plasticity of …

Trends In Public Interest In Digital Musculoskeletal Care: A Google Trends Analysis Of Hinge Health From 2020 To 2025, Nikhil Maddina, Albert D. Mousad, Fawaz Ibikunle, Minna Hassaballa, Shantanu Amin, Nicole Yeroushalmi

Student Publications

Introduction

Hinge Health (Hinge Health, Inc., San Francisco, CA) and similar musculoskeletal (MSK) telehealth platforms have grown rapidly in recent years, offering a new avenue for accessible, at-home care. As digital health further integrates into routine clinical practice, understanding public interest in these services can provide insight into shifting care-seeking behaviors and potential disparities in healthcare access. This study aims to analyze Google Trends (Google LLC, Mountain View, CA) data to evaluate temporal and geographic patterns of public search interest in Hinge Health in the United States, comparing upper and lower extremity musculoskeletal injuries, with the hypothesis that interest has …

The Perception Of Hooliganism And Its Impact Among Naia Collegiate Soccer Players, Eric Street, Fred Cromartie, Steve S. Chen, Brandon Spradley

Findings in Sport, Hospitality, Entertainment, and Event Management

There have been multiple studies with regard to the different aspects of soccer hooliganism throughout the world. Most commonly, studies of hooliganism covered areas such as harassment, vandalism, fighting, theft, public intoxication, and crowd disorder revolving around the games of soccer in various countries. This phenomenon has been studied among the major four professional sports in the United States, yet there has been no extensive research on soccer “hooliganism” associated with Major League Soccer (MLS). This study examined the survey responses from 101 National Association of Intercollegiate Athletics (NAIA) soccer players to identify their perceptions on severity of hooliganism and …

Assessing Market Potential And Swot-Based Strategies For Betong Chicken In Pattani, Yala, And Narathiwat Provinces Of Southern Thailand, Talerngsak Angkuraseranee, Chaiyawan Wattanachan, Kettawan Boonthep

The Thai Journal of Veterinary Medicine

This study aimed to evaluate market opportunities and formulate strategic pathways for the sustainable growth of Betong chicken, an indigenous premium poultry breed in southern Thailand known for its distinctive meat quality. Recent declines in the Betong chicken population and productivity have created a supply–demand imbalance, threatening the Betong chicken’s long-term economic viability. Using a mixed-methods design, data were collected from 280 stakeholders—farmers, processors, retailers, and consumers—in Pattani, Yala, and Narathiwat provinces. SWOT and Internal–External (IE) matrix analyses were applied to identify internal strengths and weaknesses and external opportunities and threats. The External Factor Evaluation (EFE) score of 2.773 indicated …

Fragments Of A Crusader-Period Chancel Screen From ‘Akko, The Knights’ Hotel Site, Vardit R. Shotten-Hallel

'Atiqot

The phrase ‘where [or: when] least expected’ is rarely used in archaeology. In fact, when excavating a cesspit, the opposite is often true. Cesspits are precisely where archaeologists expect to uncover hoards of household artifacts, like pottery and glass, or sometimes valuables, dislocated sculptures and even skeletons. In this regard, the excavation at the Knights’ Hotel site in ‘Akko is no exception. Four richly decorated marble fragments were found in a cesspit, integrated in the shaft’s wall as part of repair work. A stylistic analysis, and consideration of the historical context of their deposition, revealed an unexpected possibility regarding their …

The Metal Finds From ‘Akko, The Knights’ Hotel Site, Joppe Gosker

'Atiqot

The 2007 excavation at the Knights Hotel Site yielded about 170 metal finds, mostly iron or copper alloy, though silver and gilded items were also found. Some objects associated with warfare may have been linked to the final battle of ‘Akko in 1291 CE, but most of the finds relate to daily life, encompassing activities such as horse keeping, dress, foodways, medicine and construction. The dominance of Eastern-type arrowheads indicates that local traditions were sometimes adopted, certainly for the arrows if not the bows. Concerning dress accessories, the overall impression is of European traditions. It seems that the Franks preferred …

Crusader-Period Ceramics From The Knights’ Hotel Site, ‘Akko, Edna J. Stern, Nohar Shahar

'Atiqot

The Crusader-period ceramic assemblage unearthed in the Knights’ Hotel site in ‘Akko contains a large variety of daily wares. The assemblage consists of local and imported wares from various regions, some of which were identified through petrographic analysis. The imported wares indicate trade connections with Mediterranean port cities, and the petrographic analysis identified a new group of vessels from Egypt’s northern Nile region. The article outlines the pottery typology and quantification, and presents the ceramic distribution within the excavated urban area. The findings indicate changes in land use, as some residential and commercial areas were converted into dumps and cesspits. …

Daily Life And Industry In Crusader ‘Akko: The Knights’ Hotel Site, Danny Syon

'Atiqot

This report details the second season of excavations at the Knights’ Hotel site, carried out in 2007, covering three areas (A–C) not excavated in the first season (1995). The excavations revealed domestic and public(?) architecture dating from the thirteenth century CE. The architectural features are mostly similar to the ones exposed in the first season and include an abundance of water and sanitary installations: cisterns, wells and cesspits. The mixed character of this neighborhood of the capital of the Crusader kingdom of Jerusalem, and the daily life of its residents is highlighted by the wealth of pottery and glass vessels …

Remains Of Two Iron Age Iib–C(?) Updraft Pottery Kilns At The Foot Of Tel Gamma (Tell Jemma), Davida Eisenberg-Degen, Ilana Peters, Svetlana Talis, Yael Abadi-Reiss, Emily Bischoff Bruintjes, Yotam Asscher

'Atiqot

An excavation southwest of Tel Gamma exposed two Iron Age II updraft pottery kilns and a refuse pit. The eastern kiln was only partially preserved; its fill contained workshop debris and storage-jar wasters. The better-preserved western kiln retained an almost intact, elongated combustion chamber with two sets of mud-brick flues and evidence of periodic upkeep. An activity layer was identified between and around the kilns, from which a mixed Iron IIA–C ceramic assemblage and a few stone artifacts were collected. The stone artifacts likely served in fine grinding and burnishing. In the refuse pit, potsherds, vitrified bricks, animal bones and …

A Middle Bronze Iia Pottery Production Site On The Northern Bank Of NaḥAl Soreq, Near Yavne-Yam, Lily Singer-Avitz, Yossi Levy, Etan Ayalon

'Atiqot

A Middle Bronze Age IIA pottery production site was uncovered on the northern bank of Naḥal Soreq, near Yavne-Yam. Excavations revealed installations, including pits, an oven, a stone pavement and a two-story pottery kiln. The discovery of a potter’s wheel and petrographic analysis confirm local ceramic production using nearby ḥamra and kurkar materials. Stone anchors found on-site suggest the transport of vessels via small boats along the stream, connecting the site with nearby settlements such as Nabi Rubin and Yavne-Yam.

The Faunal Assemblage From Nahal Rimmonim, An Intermediate And Middle Bronze Age Site In The Jezreel Valley, Zohar Turgeman-Yaffe

'Atiqot

The rural site of Naḥal Rimmonim yielded 421 identifiable animal bones that originated in well-stratified loci from five strata dating to the Intermediate and Middle Bronze Ages. The site’s economy seems to have been independent, or production-based, with residents providing most of their own needs while also trading with other groups. The economy was based on animal husbandry, with some hunting, mostly of gazelles, alongside the production of secondary products, such as milk, wool and labor, with a preference for wool. Pigs were the most common species in all domestic levels, including a unique pig burial. The faunal assemblage from …

Nahal Rimmonim: An Intermediate–Middle Bronze Age Village And Pottery Workshop In The Jezreel Valley, Karen Covello-Paran, Yotam Tepper

'Atiqot

The excavations at Naḥal Rimmonim exposed a stratified rural settlement spanning the Intermediate and Middle Bronze Age III. Stratified remains—including domestic structures, installations, a pottery workshop and burials—demonstrate diachronic shifts in site function and organization. The Intermediate Bronze Age strata exhibit substantial domestic activity with evidence of localized adaptation to marshy conditions. In the Middle Bronze Age I–II phases, a specialized potters’ workshop and later domestic structures highlight evolving intrasite spatial planning and craft production, likely tied to economic interactions with nearby Tel Megiddo. Elite tombs with prestige goods from the Middle Bronze Age III, signal socioeconomic ties to urban …

Secondary Use Of Textiles For Wick Production In Light Of The Finds From The Intermediate Bronze Age Cemetery At Newe Efrayim, Naama Sukenik, Yonah Maor, Yaniv Agmon, Yossi Elisha, Gilad Itach

'Atiqot

Three Intermediate Bronze Age wicks discovered in the Newe Efrayim cemetery constitute some of the earliest known archaeological evidence of wick use, an essential object for lighting, yet lacking distinctive features, as it was intended to be burned, making it a rare find. The exceptional preservation of the wicks sheds light on both preservation issues and the technological aspects of wick production in antiquity. The wicks were made from textiles, indicating the maximal reuse of a valuable raw material after it had served its original purpose. These finds offer evidence of material recycling in the ancient world. It is likely …

Researching Chalcolithic Ivories From The Southern Levant: Craft Items For Ritual, Prestige And Exchange, Harel Shochat, Liora Kolska Horwitz, Avishay Levi-Hevroni, Martin D. Pasternak, Pablo Betzer, Daniel Varga, Yael Abadi-Reiss, Olga Negnevitsky, Ilan Naor, Ianir Milevski

'Atiqot

The Late Chalcolithic period in the Southern Levant, mainly the Ghassulian culture (mid-fifth–mid-fourth millennia BCE), heralded a new and unique trajectory in craft specialization, with ivory objects, including figurines, tools and vessels, among the outstanding ones. Hippopotamus and elephant ivory, probably of varied geographic provenience, were the raw materials employed. This paper presents two recently excavated ivory caches from the Late Chalcolithic sites of Ḥorbat Raqiq and Yavne (East), and reviews Chalcolithic ivories from this region. Additionally, a systematic microscopic analysis to determine the taxonomic identification of raw material and typological classification of each artifact is presented, offering novel perspectives …

Signaling By Intracellular Β2-Adrenergic Receptors Regulates Ampa Receptor Trafficking And Synaptic Plasticity, Boram Lee, Xiaomin Xing, Kyle E. Ireton, Bing Xu, Paul J. Gasser, Johannes W. Hell

Biomedical Sciences Faculty Research and Publications

Signaling by norepinephrine (NE) via adrenergic receptors (ARs) mediates attention, yet the underlying molecular mechanisms are largely unknown. AMPA receptors (AMPARs) form a complex with β2ARs, Gs, adenylyl cyclase, and protein kinase A (PKA) to augment AMPAR phosphorylation and, thereby, surface expression. We show that signaling by intracellular β2ARs is required for these effects and two different forms of long-term potentiation (LTP) that depend on β2AR signaling and phosphorylation of the AMPAR GluA1 subunit on S845. Inhibition of two NE transporters, the organic cation transporter 3 (OCT3) and the plasma membrane monoamine transporter (PMAT), impairs phosphorylation of the AMPAR GluA1 …

Compositions Comprising Celtos Immunogens And Antibodies And Method Of Use Thereof, Niraj Tolia, John Jimah, Nichole Salinas, Darya Urusova, John Adams, Shulin Xu, Allison Roth, Hirdesh Kumar

USF Patents

The present disclosure provides immunogenic compositions and methods for vaccination with a CelTOS immunogen. The immunogenic composition comprises Babesia, Theileria or Cytauxzoon CelTOS. The immunogenic composition may also comprise CelTOS with structural changes that affect immune recognition.

Impact Of Mining Activities On Land Use Land Cover (Lulc) Using Geospatial Techniques In The Obuasi Municipality, Jonathan Arthur Quaye-Ballard, Samira Naa Yemoley Nsiah, Jephthah Marfo Nimoh, Titus Tienaah, Tina Dzigbordi Wemegah, Precious Adwoa Okyere

Publications

Introduction: Sustainable development, which balances economic, social, and environmental factors to meet the needs of the present and future generations, is a collective responsibility. Rapid population growth and intensification of socio-economic activities are causing significant changes in Land Use Land Cover (LULC), directly impacting ecosystem services, biodiversity, and climate. The Obuasi community is already facing environmental degradation and health problems due to gold mining.

Methodology: The study assessed the spatial extent and intensity of mining activities in the Obuasi Municipality on the LULC and predicted future LULC changes due to mining activities using geospatial techniques. The LULC changes were analysed …

Effects Of Cryoprotective And Mitochondrial Stabilizing Agents On Vitrified Bovine Oocytes, Allison Hoang

LSU Master's Theses

Oocyte vitrification success is highly dependent on cryoprotectant agent (CPA) choice as well as possible additives that could help preserve mitochondrial membrane potential (mMP), reactive oxygen species (ROS) production, and calcium homeostasis within the cell. It is a delicate balance between levels needed for proper oocyte function and activation and levels needed to induce apoptotic pathways. The results of oocyte cryopreservation determine the eventual oocyte viability and subsequent fertilization and embryo development.

In this study potential permeating cryoprotectant combinations were explored such as ethylene glycol (EG) and propylene glycol (PG) or EG in combination with dimethyl sulfoxide (DMSO). Also, the …

Danicamtiv Reduces Myosin's Working Stroke But Activates The Thin Filament By Accelerating Actomyosin Attachment, Brent Scott, Lina Greenberg, Caterina Squarci, Kenneth S Campbell, Michael J Greenberg

2020-Current year OA Pubs

Heart failure is a leading cause of death worldwide, and even with current treatments, the 5-y transplant-free survival rate is only ~50 to 70%. As such, there is a need to develop new treatments for patients that improve survival and quality of life. Recently, there have been efforts to develop small molecules for heart failure that directly target components of the sarcomere, including cardiac myosin. Danicamtiv is one of these molecules; however, its direct effects on myosin's single molecule mechanics and kinetics are not well understood. Using optical trapping techniques, stopped flow transient kinetics, and in vitro reconstitution assays, we …