Digital Commons Network^™

Hypocretin/Orexin Peptides Alter Spike Encoding By Serotonergic Dorsal Raphe Neurons Through Two Distinct Mechanisms That Increase The Late Afterhyperpolarization, Masaru Ishibashi, Iryna Gumenchuk, Kenichi Miyazaki, Takafumi Inoue, William N. Ross, Christopher S. Leonard

NYMC Faculty Publications

UNLABELLED: Orexins (hypocretins) are neuropeptides that regulate multiple homeostatic processes, including reward and arousal, in part by exciting serotonergic dorsal raphe neurons, the major source of forebrain serotonin. Here, using mouse brain slices, we found that, instead of simply depolarizing these neurons, orexin-A altered the spike encoding process by increasing the postspike afterhyperpolarization (AHP) via two distinct mechanisms. This orexin-enhanced AHP (oeAHP) was mediated by both OX1 and OX2 receptors, required Ca(2+) influx, reversed near EK, and decayed with two components, the faster of which resulted from enhanced SK channel activation, whereas the slower component decayed like a slow AHP …

Perceptions Of Active Learning Between Faculty And Undergraduates: Differing Views Among Departments, Lorelei E. Patrick, Leigh Ann Howell, William Wischusen

Biological Sciences Faculty Publications

There have been numerous calls recently to increase the use of active learning in university science, technology, engineering, and math (STEM) classrooms to more actively engage students and enhance student learning. However, few studies have investigated faculty and student perceptions regarding the effectiveness of active learning or the barriers to its implementation. Previous work surveying a single class in a single department has suggested that faculty and students have different perceptions of the effectiveness of active learning strategies and the barriers faculty face when implementing these teaching strategies. We expand on these previous findings by surveying a larger and more …

Cracking Diversity’S Code, University Of Dayton

News Releases

The National Science Foundation has awarded University of Dayton associate professor of biology Tom Williams an $839,000 grant to research how evolution used so-called “genetic switches” to develop biological diversity.

Prevalence And Determinants Of Metabolic Syndrome In Qatar: Results From A National Health Survey, Mohamed Al-Thani, Al Anoud M. Al-Thani, Sohaila Cheema, Javaid Sheikh, Ravinder Mamtani, Albert B. Lowenfels, Walaa F. Al-Chetachi, Badria Almalki, Shamseldin A. Hassan Khalifa, Ahmad O. Haj Bakri, Patrick Maisonneuve

NYMC Faculty Publications

OBJECTIVES: To determine optimum measurements for abdominal obesity and to assess the prevalence and determinants of metabolic syndrome in Qatar.

DESIGN: National health survey.

SETTING: Qatar National STEPwise Survey conducted by the Supreme Council of Health during 2012.

PARTICIPANTS: 2496 Qatari citizens aged 18-64 representative of the general population.

PRIMARY AND SECONDARY OUTCOME MEASURES: Measure of obesity (body mass index, waist circumference or waist-to-height ratio) that best identified the presence of at least 2 other factors of metabolic syndrome; cut-off values of waist circumference; frequency of metabolic syndrome.

RESULTS: Waist circumference ≥102 for men and ≥94 cm for women was …

Microrna-140 Is Elevated And Mitofusin-1 Is Downregulated In The Right Ventricle Of The Sugen5416/Hypoxia/Normoxia Model Of Pulmonary Arterial Hypertension, Sachindra Raj Joshi, Vidhi Dhagia, Salina Gairhe, John G. Edwards, Ivan F. Mcmurtry, Sachin A. Gupte

NYMC Faculty Publications

Heart failure, a major cause of morbidity and mortality in patients with pulmonary arterial hypertension (PAH), is an outcome of complex biochemical processes. In this study, we determined changes in microRNAs (miRs) in the right and left ventricles of normal and PAH rats. Using an unbiased quantitative miR microarray analysis, we found 1) miR-21-5p, miR-31-5 and 3p, miR-140-5 and 3p, miR-208b-3p, miR-221-3p, miR-222-3p, miR-702-3p, and miR-1298 were upregulated (>2-fold; P < 0.05) in the right ventricle (RV) of PAH compared with normal rats; 2) miR-31-5 and 3p, and miR-208b-3p were upregulated (>2-fold; P < 0.05) in the left ventricle plus septum (LV+S) of PAH compared with normal rats; 3) miR-187-5p, miR-208a-3p, and miR-877 were downregulated (>2-fold; P < 0.05) in the RV of PAH compared with normal rats; and 4) no miRs were up- or downregulated with >2-fold in LV+S compared with RV of PAH and normal. Upregulation of miR-140 and miR-31 in the hypertrophic RV was further confirmed by quantitative PCR. …

Chemistry And Biochemistry News, Georgia Southern University

Biochemistry, Chemistry & Physics: News & Publications (2012-2023)

• Faculty Awards for 2015-2016
• Georgia Southern's Elite 8 for REU-2016

Colorectal Cancer On The Decline, Albert B. Lowenfels, Patrick Maisonneuve, Ravinder Mamtani

NYMC Faculty Publications

No abstract provided.

Deep Phylogenomics Of A Tandem-Repeat Galectin Regulating Appendicular Skeletal Pattern Formation, Ramray Bhat, Mahul Chakraborty, Tilmann Glimm, Thomas A. Stewart, Stuart A. Newman

NYMC Faculty Publications

BACKGROUND: A multiscale network of two galectins Galectin-1 (Gal-1) and Galectin-8 (Gal-8) patterns the avian limb skeleton. Among vertebrates with paired appendages, chondrichthyan fins typically have one or more cartilage plates and many repeating parallel endoskeletal elements, actinopterygian fins have more varied patterns of nodules, bars and plates, while tetrapod limbs exhibit tandem arrays of few, proximodistally increasing numbers of elements. We applied a comparative genomic and protein evolution approach to understand the origin of the galectin patterning network. Having previously observed a phylogenetic constraint on Gal-1 structure across vertebrates, we asked whether evolutionary changes of Gal-8 could have critically …

Deep Phylogenomics Of A Tandem-Repeat Galectin Regulating Appendicular Skeletal Pattern Formation, Ramray Bhat, Mahul Chakraborty, Tilmann Glimm, Thomas A. Stewart, Stuart A. Newman

NYMC Faculty Publications

BACKGROUND: A multiscale network of two galectins Galectin-1 (Gal-1) and Galectin-8 (Gal-8) patterns the avian limb skeleton. Among vertebrates with paired appendages, chondrichthyan fins typically have one or more cartilage plates and many repeating parallel endoskeletal elements, actinopterygian fins have more varied patterns of nodules, bars and plates, while tetrapod limbs exhibit tandem arrays of few, proximodistally increasing numbers of elements. We applied a comparative genomic and protein evolution approach to understand the origin of the galectin patterning network. Having previously observed a phylogenetic constraint on Gal-1 structure across vertebrates, we asked whether evolutionary changes of Gal-8 could have critically …

Newsroom, Georgia Southern University

Newsroom (2006-2023)

• Campus safety at Georgia Southern is within closer reach with LiveSafe app
• '2016 Faculty Exhibition' Opens at Center for Art & Theatre
• Lecture on immigration detention and human rights defenders on Aug. 18
• Georgia Southern brings renewable energy research to schools in high-need rural Georgia
• Georgia Southern faculty, staff gear up for fall semester

Linking Inflammation And Parkinson Disease: Hypochlorous Acid Generates Parkinsonian Poisons, Thomas M. Jeitner, Mike Kalogiannis, Boris F. Krasnikov, Irving Gomolin, Morgan R. Peltier, Graham R. Moran

NYMC Faculty Publications

Inflammation is a common feature of Parkinson Disease and other neurodegenerative disorders. Hypochlorous acid (HOCl) is a reactive oxygen species formed by neutrophils and other myeloperoxidase-containing cells during inflammation. HOCl chlorinates the amine and catechol moieties of dopamine to produce chlorinated derivatives collectively termed chlorodopamine. Here, we report that chlorodopamine is toxic to dopaminergic neurons both in vivo and in vitro Intrastriatal administration of 90 nmol chlorodopamine to mice resulted in loss of dopaminergic neurons from the substantia nigra and decreased ambulation-results that were comparable to those produced by the same dose of the parkinsonian poison, 1-methyl-4-phenylpyridinium (MPP+). Chlorodopamine was …

Female-Favorable Attenuation Of Coronary Myogenic Constriction Via Reciprocal Activations Of Epoxyeicosatrienoic Acids And Nitric Oxide, Ghezal Froogh, Jun Qin, Sharath Kandhi, Yicong Le, Houli Jiang, Meng Luo, Dong Sun, An Huang

NYMC Faculty Publications

Epoxyeicosatrienoic acids (EETs) are metabolites of arachidonic acid via CYP/epoxygenases, which are catabolized by soluble epoxide hydrolase (sEH) and known to possess cardioprotective properties. To date, the role of sEH in the modulation of pressure-induced myogenic response/constriction in coronary arteries, an important regulatory mechanism in the coronary circulation, and the issue as to whether the disruption of the sEH gene affects the myogenic response sex differentially have never been addressed. To this end, experiments were conducted on male (M) and female (F) wild-type (WT) and sEH-knockout (KO) mice. Pressure-diameter relationships were assessed in isolated and cannulated coronary arteries. All vessels …

Pacific Review Summer 2016, Alumni Association Of The University Of The Pacific

Pacific Magazine and Pacific Review

No abstract provided.

Endostatin And Transglutaminase 2 Are Involved In Fibrosis Of The Aging Kidney, Chi Hua Lin, Jun Chen, Zhongtao Zhang, Gail V. Johnson, Arthur J. Cooper, Julianne Feola, Alexander Bank, Jonathan Shein, Heli J. Ruotsalainen, Taina A. Pihlajaniemi, Michael S. Goligorsky

NYMC Faculty Publications

Endostatin (EST), an antiangiogenic factor, is enriched in aging kidneys. EST is also an interactive partner of transglutaminase 2 (TG2), an enzyme that cross-links extracellular matrix proteins. Here we tested whether EST and TG2 play a role in the fibrosis of aging. In wild-type mice, aging kidneys exhibited a 2- to 4-fold increase in TG2 paralleled by increased cross-linked extracellular matrix proteins and fibrosis. Mice transgenic to express EST showed renal fibrosis at a young age. One-month delivery of EST via minipumps to young mice showed increased renal fibrosis that became more robust when superimposed on folic acid-induced nephropathy. Upregulated …

Improved Student Learning Through A Faculty Learning Community: How Faculty Collaboration Transformed A Large-Enrollment Course From Lecture To Student Centered, Emily R. Elliott, Robert D. Reason, Clark R. Coffman, Eric J. Gangloff, Jeffrey R Raker, Jo Anne Powell-Coffman, Craig A. Ogilvie

Chemistry Faculty Publications

Undergraduate introductory biology courses are changing based on our growing understanding of how students learn and rapid scientific advancement in the biological sciences. At Iowa State University, faculty instructors are transforming a second-semester large-enrollment introductory biology course to include active learning within the lecture setting. To support this change, we set up a faculty learning community (FLC) in which instructors develop new pedagogies, adapt active-learning strategies to large courses, discuss challenges and progress, critique and revise classroom interventions, and share materials. We present data on how the collaborative work of the FLC led to increased implementation of active-learning strategies and …

How Diet Intervention Via Modulation Of Dna Damage Response Through Micrornas May Have An Effect On Cancer Prevention And Aging, An In Silico Study, Felicia Carotenuto, Maria C. Albertini, Dario Coletti, Alessandra Vilmercati, Luigi Campanella, Zbigniew Darzynkiewicz, Laura Teodori

NYMC Faculty Publications

The DNA damage response (DDR) is a molecular mechanism that cells have evolved to sense DNA damage (DD) to promote DNA repair, or to lead to apoptosis, or cellular senescence if the damage is too extensive. Recent evidence indicates that microRNAs (miRs) play a critical role in the regulation of DDR. Dietary bioactive compounds through miRs may affect activity of numerous genes. Among the most studied bioactive compounds modulating expression of miRs are epi-gallocatechin-3-gallate, curcumin, resveratrol and n3-polyunsaturated fatty acids. To compare the impact of these dietary compounds on DD/DDR network modulation, we performed a literature search and an in …

2016 Great Expectations Spring Faculty Conference: Inclusive Teaching, Diverse Classrooms, Academic Affairs

Spring Great Expectation Faculty Conference

The 2016 Great Expectations Spring Faculty Conference featured a keynote speech by Dr. Lisa Guion Jones.

Dr. Lisa Guion Jones has nearly 23 years of experience in higher education. She currently serves as Professor and Assistant Dean in the College of Agriculture and Life Sciences at North Carolina State University. In this role, she provides strategic leadership to Goal 2 of the College’s Strategic Plan, to "exhibit an inclusive and diverse environment where faculty, staff and students can each reach their full potential."

As a transformational leader, Dr. Jones has initiated innovative programs that have increased the recruitment and retention …

Eets Elicit Direct Increases In Pulmonary Arterial Pressure In Mice, Sharath Kandhi, Ghezal Froogh, Jun Qin, Meng Luo, Michael S. Wolin, An Huang, Dong Sun

NYMC Faculty Publications

OBJECTIVE: The biological role of epoxyeicosatrienoic acids (EETs) in the regulation of pulmonary circulation is currently under debate. We hypothesized that EETs initiate increases in right ventricular systolic pressure (RVSP) via perhaps, pulmonary vasoconstriction. METHODS: Mice were anesthetized with isoflurane. Three catheters, inserted into the left jugular vein, the left carotid artery, and the right jugular vein, were used for infusing EETs, monitoring blood pressure (BP), and RVSP respectively. BP and RVSP were continuously recorded at basal conditions, in response to administration of 4 regioisomeric EETs (5,6-EET; 8,9-EET; 11,12-EET, and 14,15-EET; 1, 2, 5 and 10 ng/g body weight (BW) …

20-Hete-Induced Mitochondrial Superoxide Production And Inflammatory Phenotype In Vascular Smooth Muscle Is Prevented By Glucose-6-Phosphate Dehydrogenase Inhibition, Anand Lakhkar, Vidhi Dhagia, Sachindra Raj Joshi, Katherine Gotlinger, Dhara Patel, Dong Sun, Michael S. Wolin, Michal L. Schwartzman, Sachin A. Gupte

NYMC Faculty Publications

20-Hydroxyeicosatetraeonic acid (20-HETE) produced by cytochrome P-450 monooxygenases in NADPH-dependent manner is proinflammatory, and it contributes to the pathogenesis of systemic and pulmonary hypertension. In this study, we tested the hypothesis that inhibition of glucose-6-phosphate dehydrogenase (G6PD), a major source of NADPH in the cell, prevents 20-HETE synthesis and 20-HETE-induced proinflammatory signaling that promotes secretory phenotype of vascular smooth muscle cells. Lipidomic analysis indicated that G6PD inhibition and knockdown decreased 20-HETE levels in pulmonary arteries as well as 20-HETE-induced 1) mitochondrial superoxide production, 2) activation of mitogen-activated protein kinase 1 and 3, 3) phosphorylation of ETS domain-containing protein Elk-1 that …

Sirtuins, Cell Senescence, And Vascular Aging, Yujiro Kida, Michael S. Goligorsky

NYMC Faculty Publications

The sirtuins (SIRTs) constitute a class of proteins with nicotinamide adenine dinucleotide-dependent deacetylase or adenosine diphosphate-ribosyltransferase activity. Seven SIRT family members have been identified in mammals, from SIRT1, the best studied for its role in vascular aging, to SIRT7. SIRT1 and SIRT2 are localized in the nucleus and cytoplasm. SIRT3, SIRT4, and SIRT5 are mitochondrial, and SIRT6 and SIRT7 are nuclear. Extensive studies have clearly revealed that SIRT proteins regulate diverse cell functions and responses to stressors. Vascular aging involves the aging process (senescence) of endothelial and vascular smooth muscle cells. Two types of cell senescence have been identified: (1) …

Assessing Next-Generation Sequencing And 4 Bioinformatics Tools For Detection Of Enterovirus D68 And Other Respiratory Viruses In Clinical Samples, Weihua Huang, Guiqing Wang, Henry Lin, Jian Zhuge, Sheila M. Nolan, Eric Vail, Nevenka Dimitrova, John T. Fallon

NYMC Faculty Publications

We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

Annual Report, College Of Arts & Sciences Faculty Council, 2015-2016, University Of South Florida St. Petersburg. College Of Arts & Sciences

College Council Meeting Minutes

Annual report for 2015-2016 for the University of South Florida St. Petersburg College of Arts and Sciences' Council.

Final Progress Report California Open Educational Resources Council, Katherine Harris

Faculty Research, Scholarly, and Creative Activity

Submitted to the Intersegmental Committee of Academic Senates December 1, 2015 (rev 4/15/16) Permalink: http://tinyurl.com/FPRCAOERC41516 Printable PDF Version: http://tinyurl.com/FPRCAOERC41516pdf See also CA-OERC White Paper: OER Adoption in College Classrooms

The Loop (Spring 2016), Taylor University

The Loop (2012-2017)

The late Spring 2016 edition of Taylor University’s The Loop.

Serotonin As A Putative Scavenger Of Hypohalous Acid In The Brain, Mike Kalogiannis, E James Delikatny, Thomas M. Jeitner

NYMC Faculty Publications

Neurodegenerative disorders represent the culmination of numerous insults including oxidative stress. The long etiology of most of these disorders suggests that lessening the effects of one or more of the insults could significantly delay disease onset. Antioxidants have been tested as possible therapeutics for neurodegenerative disorders, but with little success. Here we report that serotonin acts as a scavenger of hypochlorous acid (HOCl) in the brain. Serotonin was shown to prevent the oxidation of 2-thio-5-nitrobenzoate by HOCl in a biphasic manner. The first phase was a partial scavenging that occurred at concentrations of serotonin that exceeded those of HOCl. (1)H-NMR …

Translating Genomic Advances To Physical Therapist Practice: A Closer Look At The Nature And Nurture Of Common Diseases, Catherine L. Curtis, Allon Goldberg, Jeffrey A. Kleim, Steven L. Wolf

NYMC Faculty Publications

The Human Genome Project and the International HapMap Project have yielded new understanding of the influence of the human genome on health and disease, advancing health care in significant ways. In personalized medicine, genetic factors are used to identify disease risk and tailor preventive and therapeutic regimens. Insight into the genetic bases of cellular processes is revealing the causes of disease and effects of exercise. Many diseases known to have a major lifestyle contribution are highly influenced by common genetic variants. Genetic variants are associated with increased risk for common diseases such as cardiovascular disease and osteoarthritis. Exercise response also …

Central Role Of Glutamate Metabolism In The Maintenance Of Nitrogen Homeostasis In Normal And Hyperammonemic Brain, Arthur J. Cooper, Thomas M. Jeitner

NYMC Faculty Publications

Glutamate is present in the brain at an average concentration-typically 10-12 mM-far in excess of those of other amino acids. In glutamate-containing vesicles in the brain, the concentration of glutamate may even exceed 100 mM. Yet because glutamate is a major excitatory neurotransmitter, the concentration of this amino acid in the cerebral extracellular fluid must be kept low-typically µM. The remarkable gradient of glutamate in the different cerebral compartments: vesicles > cytosol/mitochondria > extracellular fluid attests to the extraordinary effectiveness of glutamate transporters and the strict control of enzymes of glutamate catabolism and synthesis in well-defined cellular and subcellular compartments in the …

Dimensionality Reduction Based Optimization Algorithm For Sparse 3-D Image Reconstruction In Diffuse Optical Tomography, Tanmoy Bhowmik, Hanli Liu, Zhou Ye, Soontorn Oraintara

Bioengineering Faculty Publications - Archive

No abstract provided.

20-Hete Activates The Transcription Of Angiotensin-Converting Enzyme Via Nuclear Factor-Κb Translocation And Promoter Binding, Victor Garcia, Brian Shkolnik, Laura Milhau, John R. Falck, Michal Laniado Schwartzman

NYMC Faculty Publications

Increased vascular 20-hydroxyeicosatetraenoic acid (20-HETE), a cytochrome P450 arachidonic acid metabolite, promotes vascular dysfunction, injury, and hypertension that is dependent, in part, on the renin angiotensin system (RAS). We have shown that, in human microvascular endothelial cells, 20-HETE increases angiotensin-converting enzyme (ACE) mRNA, protein, and ACE activity via an epidermal growth factor receptor (EGFR)/tyrosine kinase/mitogen-activated protein kinase (MAPK)/inhibitor of κB kinase (IKK)β-mediated signaling pathway. In this work, we show that, similar to epidermal growth factor (EGF), 20-HETE (10 nM) activates EGFR by stimulating tyrosine phosphorylation; however, unlike 20-HETE, EGF does not induce ACE expression, and pretreatment with a neutralizing antibody …

Thomas Griffiths, Thomas Griffiths, Meg Miner

All oral histories

Griffiths recalls that Myers had the "urge to collect" and built collections purposefully, even to the point of paying a higher price to fill a gap. Recalls Myers discarding books, one of which he has, but suspects it was only if Minor had duplicates. Reflects on the influence Myers had on faculty, through emphasis on and support for scholarship, and on students and facilities. Also discusses being present during meetings about the book auction, the last conversation he had with Myers and the one book he received as a gift after Myers' illness. The idea of wearing chrysanthemums at the …