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The Role Of Insulin-Like Growth Factor I In The Development Of The Cerebellum In Euthyroid And Hypothyroid Mice, Prathibha Mangedarage

All Graduate Theses, Dissertations, and Other Capstone Projects

Thyroid hormone deficiency during development is known to cause anatomical defects in the cerebellum that lead to neurological deficits. It has been observed that insufficient thyroid hormone levels also decrease the levels of brain derived Insulin-like growth factor 1 (Igf-1). Given that the deficits caused by low Igf-1 levels are similar to those caused by low thyroid hormone, we designed this study to investigate if the deficits caused in the development of the cerebellum by low thyroid hormone levels may be due to the lack of sufficient Igf-1. We also sought to determine if ectopic over expression of Igf-1 in …

Investigation Of Glycoside Hydrolases And Calcium In Cytophaga Hutchinsonii Cellulose Utilization, Choua Kou Vang

All Graduate Theses, Dissertations, and Other Capstone Projects

Cytophaga hutchinsonii is a soil bacterium that can utilize cellulose as a carbon source. The cellulose utilization system of C. hutchinsonii has been predicted to be novel and its endoglucanases could potentially be used in the production of cellulosic based biofuels. These enzymes could be used to process cellulose to glucose or other soluble sugars, which can be further converted to ethanol by yeast fermentation. Current biofuels utilize starches, primarily from corn, which has direct competition with farming space for food crops. This study focuses on observing the growth of C. hutchinsonii knockout mutant strains on cellulose substrate to better …

How Akron Affects The Water Quality Of The Cuyahoga River, Allyson Darst

Williams Honors College, Honors Research Projects

The purpose of this study was to analyze whether the city of Akron had negative effects on the Cuyahoga river, and determine if the Cuyahoga River was naturally able to remediate these effects downstream. The pollutants measured in this study include hardness, bromine, residual chlorine, iron, copper, lead, nitrate, nitrite, ammonium chloride, total chlorine, fluoride, carbonate, pH, total alkalinity and cyanuric acid. This study was done using water testing strips (JNW Direct) to test the water at four different locations along the Cuyahoga River downstream (North) of Akron. This study found that the city of Akron does have a negative …

To What Extent Do Non-Native Shrubs Support Higher Trophic Levels?, Ari Zakroff

Browse all Theses and Dissertations

Invasive species threaten ecosystems and economies. Globally, biological invasions are estimated to have cost over $2.1 trillion since 1970. In Eastern North American woodlands, invasive plants are rapidly displacing natives. This is concerning, because invasive plants may not support the diverse and abundant arthropod communities essential to ecosystem function. Despite the conceptual understanding of invasive shrubs’ potential to transform forest communities, scant research has focused on the effect of invasive plants on higher trophic levels here in Ohio. To address this gap, I examined the diversity and abundance of arthropod communities, caterpillar performance, and caterpillar predation on two invasive shrubs, …

Systematics, Taxonomy, And Ecology Of Neotropical Tachinidae (Diptera) With Focus On The Tribe Polideini, Juan Manuel Perilla López

Browse all Theses and Dissertations

The diverse family of flies Tachinidae are a significant component of insect communities in nearly all terrestrial systems from deserts to tropical forests to the high arctic. Due to their parasitoid habit, tachinids are important enemies of a variety of other insect groups. Despite the apparent importance of this group, current studies—including ecological and biogeographic studies—are limited by taxonomic and systematic impediments, including unknown phylogenetic frameworks for most tribes, poorly known generic limits, and a large number of undescribed tropical species. Here I present studies in taxonomy, systematics, phylogenetics and ecology of the Polideini of the Neotropical region organized in …

Lipin1 Improves Membrane Integrity In Dystrophic Muscles Of Mdx Mice, Abdulrahman Jama

Browse all Theses and Dissertations

Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder that affects 1 in 3500 male births worldwide. It is characterized by mutations in the dystrophin gene that results in the loss of functional dystrophin. Dystrophin deficiency leads to instability of the sarcolemma alongside increased inflammation, necrosis and fibrosis resulting in membrane rupture and eventual muscle fiber death. There are currently no effective treatments for DMD. Here we show that Lipin1 expression is significantly downregulated at the mRNA and protein levels in gastrocnemius muscle of mdx mice, the DMD mouse model. Lipin1 has a dual function as a phosphatidic acid phosphatase …

A Fecal Survey To Evaluate The Prevalence Of Enteric Viruses In Laboratory Mice, Amina Khatun

Browse all Theses and Dissertations

The study was conducted to investigate the prevalence of four enteric viruses in mouse research facilities that may not be captured by routine pathogen screening. Fecal samples were collected from contact-sentinel mouse cages between April 2022 and January 2023, and screened for the presence of viral genome by RT-qPCR. Out of a total of 50 samples, murine norovirus and murine astrovirus were detected in two (4%) and one (2%) sample, respectively, while murine adenovirus and murine rotavirus were not detected. Furthermore, all samples were screened for the presence of infectious murine noroviruses and astroviruses using the RAW264.7 and Vero cells …

Characterization Of The Roles Of Mitochondria In The Toxicity Of Α-Synuclein In A Respiratory Cell Model, Breonna Elizabeth Gillespie

Browse all Theses and Dissertations

Although α-synuclein (α-syn) has been linked genetically and pathologically to Parkinson’s disease, the mechanism by which it drives disease pathogenesis is not fully understood. When α-syn is overexpressed in yeast, it forms abnormal cytoplasmic accumulations and leads to cell death, resembling the phenotypes observed in neurons. Such yeast models have been widely used to uncover molecular mechanisms of α-syn toxicity, but the induction of α-syn relies on growing yeast in galactose, a fermentable carbon source. Yeast does not require cellular respiration when grown in galactose, thus limiting the understanding of α-syn toxicity when cellular respiration is essential for neurons. We …

A Novel Role Of Lipin1 In The Regulation Of Expression And Function Of Nnos, Ayat Azzam

Browse all Theses and Dissertations

Duchenne muscular dystrophy (DMD) is a severe and progressive muscular dystrophy that develops in the skeletal muscles because of mutations in the dystrophin gene. Dystrophin stabilizes sarcolemma and assembles neuronal nitric oxide synthase (nNOS) into the dystrophin-associated protein complex on the sarcolemma. The absence of dystrophin triggers the delocalization of nNOS and contributes to the misregulation of muscle development, blood flow, muscle fatigue, and inflammation. Lipin1 was reduced in the skeletal muscles of patients with DMD and the mdx mouse model of DMD. In this study, we explored the role of lipin1 in the regulation of nNOS expression and function. …

The Role Of Machine Learning And Network Analyses In Understanding Microbial Composition In An Experimental Prairie, Ali Eastman Oku

Graduate Research Theses & Dissertations

Machine learning and network analyses are powerful modern tools can process and map out connections between large amount of ecological data from complex environmental communities. Random forests, an ensemble machine learning algorithm, are particularly powerful as they can capture complex patterns in data while remaining easily interpretable. These tools are specifically useful in experimental settings where different types of data are collected. The aim of this study was to demonstrate the utility of machine learning models and network analyses at analyzing diverse ecological data from dynamic plant-soil microbial communities in a prairie ecosystem. Our experimental system is an experimental prairie …

Molecular And Enzymatic Resistance Of Culex Tarsalis To Pyrethroid And Organophosphate Insecticides, William Mortola

University of the Pacific Theses and Dissertations

Culex tarsalis, a vector for diseases like West Nile virus, is evolving resistance to pyrethroid and organophosphate insecticides used for agriculture and vector control in Northern California. Common mechanisms of resistance in other Culex species include the target-site mutations kdr and ace-1 and increased levels of detoxification enzymes (esterases, GSTs, and oxidases). This study contained two parts: Pyrethroid Individual study and an Organophosphate Preliminary study. For the Pyrethroid Individual study, the goal was to compare the prevalence of kdr mutations and detoxification enzymes between pyrethroid susceptible and resistant Cx. tarsalis individuals in Northern California. For the Organophosphate Preliminary study, the …

Proposing An Rna Interference (Rnai)-Based Treatment For Human Immunodeficiency Virus (Hiv) By Analyzing The Post-Transcriptional Gene Targeting Of Sars-Cov-2, Hepatitis C Virus, And A549 Lung Cancer Cells, Arjun Jagdeesh

UROP Posters

Human Immunodeficiency Virus (HIV) is a retrovirus that infects CD4+ T cell lymphocytes in humans, leading to the development of Acquired Immunodeficiency Syndrome (AIDS) if left untreated. While current treatment methods, including antiretroviral combination treatments, effectively limit HIV replication, HIV can evade these treatments due to its high mutation rate. Long-term antiretroviral treatment can also be toxic to patients, meaning patients would benefit from a new mechanism of HIV treatment. RNA interference (RNAi) is an antiviral pathway found in mammals, plants, and insects that involves a small-interfering RNA that is incorporated into a protein complex called the RNA-induced Silencing Complex …

Warm Land Surface Temperatures And Eastern Asian Homo, Robert Patalano, Hong Yang

Biological and Biomedical Sciences Department Faculty Journal Articles

Climate change and hominin evolution are inextricably linked. Pleistocene climate variability, for example, is thought to have had major influences on hominin morphology, brain size, and diversity. However, clear cause-and-effect relationships between specific climatic events and major evolutionary occurrences are difficult to establish due to temporal and spatial gaps in paleoclimatic, paleoenvironmental, and archaeological records. A new branched GDGT paleotemperature record from the Lantian Basin of Central China (Lu et al., 2022), a location known for the earliest hominin presence in East Asia, illustrates warm land surface temperatures over a two-million-year period between 2.6 and 0.6 Ma, a critical time …

Exceptional Larval Morphology Of Nine Species Of The Anastrepha Mucronota Species Group (Diptera: Tephritidae), Erick J. Rodriguez, Gary J. Steck, Matthew R. Moore, Allen L. Norrbom, Jessica Diaz, Louis A. Somma, Raul Ruiz-Arce, Bruce D. Sutton, Norma Nolazco, Alies Muller, Marc A. Branham

Papers in Evolution

Anastrepha is the most diverse and economically important genus of Tephritidae in the American tropics and subtropics. The striking morphology of the third instars of Anastrepha caballeroi Norrbom, Anastrepha crebra Stone, Anastrepha haplacantha Norrbom & Korytkowski, Anastrepha korytkowskii Norrbom, Anastrepha nolazcoae Norrbom & Korytkowski, and three newly discovered and as yet formally unnamed species (Anastrepha sp. Peru-82, Anastrepha sp. nr. protuberans, and Anastrepha sp. Sur-16), and the more typical morphology of Anastrepha aphelocentema Stone, are described using light and scanning electron microscopy. To contribute to a better understanding of the interspecific and intraspecific variation among species in the mucronota …

Fall/Winter 2022: Fund Of Knowledge, Trisha Lawless

St. Norbert College Magazine 2019-2023

A magnanimous total of five National Science Foundation grants are among multiple sources currently financing cutting-edge research at St. Norbert. The thrill of discovery is an essential part of the educational journey for students and faculty at St. Norbert College. It’s a journey in which the college finds willing partners – among them the National Science Foundation (NSF). SNC currently has five active research grants from the influential federal agency.

Improving Students' Critical Thinking In Biology Learning Through Reading, Concept Mapping-Team Quiz, Meiliana Dwi Cahya, Siti Zubaidah, Munzil Munzil, Azizul Ghofar Candra Wicaksono

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

This study aimed to examine an increase in students' critical thinking in biology following the implementation of the Reading Concept Mapping-Team Quiz (Remap-TQ). The study employed a quasi-experimental pretest-posttest non-equivalent control group design. The research population contained all eleventh graders from seven science classes at SMAN 2 Tanggul Jember, East Java, and the total number of respondents was 105 students. This study's critical thinking indicators include applying, evaluating, using data to develop critical insight, analysing, and synthesising. The analysis showed that the Remap-TQ students achieved better than the TQ and conventional students in terms of critical thinking, indicated by their …

The Role Of Estrogen Signaling In Sexually Dimorphic Adipose Expansion, Rocío Del Mar Saavedra-Peña

Yale Graduate School of Arts and Sciences Dissertations

In obesity, white adipose tissue (WAT) distribution differs between the sexes, with men preferentially accumulating visceral WAT (VWAT) and females exhibiting subcutaneous WAT (SWAT) accumulation bias. Obesogenic adipocyte hyperplasia (increase in adipocyte number) contributes to the sexually dimorphic pattern of fat distribution in mice driven by cues in the tissue microenvironment, with females displaying hyperplasia in both VWAT and SWAT, while males have VWAT-specific hyperplasia, implicating a role for sex hormones in this process during obesity. Sex hormones play a pivotal role in physiology and disease. Estrogen, the female sex hormone, has been long implicated in having protective roles against …

Investigating A Locomotor Neuron That Makes A Potential Sensory Cilium Lying Over The C. Elegans Egg-Laying Apparatus, Designing An Accessible System For Neuron Identifications, And Advancing Imaging And Modeling In The Egg-Laying System, Nakeirah Tm Christie

Yale Graduate School of Arts and Sciences Dissertations

The neural circuit for Caenorhabditis elegans egg laying has been studied intensively for decades, yet its known components cannot account for how egg laying and locomotion behaviors are coordinated. In my graduate work within the Koelle lab, I worked to further complete that model circuit. The first half of this dissertation covers my work on the PVP neurons. I discovered that the PVP neuron pair, which has previously been implicated in locomotion, makes previously-undescribed branches that terminate in large wing-shaped endings directly over the egg-laying apparatus. The PVP wing structures occur only in hermaphrodites and develop during the L4 larval …

A Novel Piggyback Strategy For Mrna Delivery Exploiting Adenovirus Entry Biology, Myungeun Lee, Paul J Rice-Boucher, Logan Thrasher Collins, Ernst Wagner, Lorenzo Aulisa, Jeffrey Hughes, David T Curiel

2020-Current year OA Pubs

Molecular therapies exploiting mRNA vectors embody enormous potential, as evidenced by the utility of this technology for the context of the COVID-19 pandemic. Nonetheless, broad implementation of these promising strategies has been restricted by the limited repertoires of delivery vehicles capable of mRNA transport. On this basis, we explored a strategy based on exploiting the well characterized entry biology of adenovirus. To this end, we studied an adenovirus-polylysine (AdpL) that embodied "piggyback" transport of the mRNA on the capsid exterior of adenovirus. We hypothesized that the efficient steps of Ad binding, receptor-mediated entry, and capsid-mediated endosome escape could provide an …

Stem Cell Modelling Of Human Disease, Kristian Kennedy

Student Research and Creative Projects 2021-2022

The purpose of the project was the establish an iPSC line at WSU to provide current and future students with stem cell culture training and experience. Another purpose of this project was to optimize freezing, thawing, propagation, and neural differentiation protocols for future students to use at WSU.

Short-Eared Owl Land-Use Associations During The Breeding Season In The Western United States, Robert A. Miller, Joseph B. Buchanan, Theresa L. Pope, Jay D. Carlisle, Colleen E. Moulton, Travis L. Booms

Intermountain Bird Observatory Publications and Presentations

The Short-eared Owl (Asio flammeus) is a species of conservation concern in the western USA, with evidence for declining population sizes. Monitoring of Short-eared Owls is complicated because of their low site fidelity and nomadic movements. We recruited community-science participants to implement a multi-year survey of Short-eared Owls across eight states in the western USA, resulting in a program of sufficient temporal and spatial dimensions to overcome many of the challenges in monitoring this species. We implemented both multi-scale occupancy and colonization/extinction modeling to provide insights into land-cover use, and to identify which cover types supported higher occurrence. …

Importance Of Flood Irrigation For Foraging Colonial Waterbirds, Colleen E. Moulton, Jay D. Carlisle, Sonya J. Knetter, Kathryn Brenner, Robert A. Cavallaro

Intermountain Bird Observatory Publications and Presentations

For a colonial‐nesting bird, like the white‐faced ibis (Plegadis chihi), the landscape surrounding the breeding colony can be important. White‐faced ibis must rely on areas outside their breeding colony for foraging, but this part of their life history has received little attention, and the management of this landscape even less so. To address this knowledge gap, we conducted road‐based driving surveys and a randomly selected, spatially balanced sample survey of agricultural fields within a 22‐km radius of the 2 largest white‐ faced ibis breeding colonies in Idaho, USA: Market Lake Wildlife Management Area and Mud Lake Wildlife Management …

Exploring Biology Beyond The Classroom, Staff Report

University of Mississippi News

Off-campus courses give students hands-on opportunities in biodiversity locations

Evaluating Bat Roost Abundance: A Comparison Of Drone-Acquired Thermal Imagery And Acoustic Estimates With Visual Observations, Karah Jaffe

Electronic Theses and Dissertations

Roosts provide an opportunity to census philopatric populations of bats. Comparing the efficacy of traditional methods with novel methods can provide guidance on the use of new technologies in the field. This project aims to compare the count efficacy of external emergence methodologies, i.e., drone-acquired thermal imagery, acoustic estimates, and visual counts. Surveys were conducted ten nights at two emergence sites and synchronized to compare counts. Acoustic estimates could not be established as there was a weak prediction of the linear relationship between root-mean-square pressure and emergence count; therefore, this method was removed from the comparative analysis. A linear mixed-effects …

Bio 120: General Biology Oer Curation, Erica L. Schiller

Curated OER Collections

This OER curation is an annotated bibliography of prospective OER for the GVSU course BIO 120: General Biology.

Investigating Roles Of 2 Novel Eklf Targets Involved In Erythropoiesis, Rose M. Gott

ETD Archive

Erythrocytes are primarily comprised of the oxygen carrying protein hemoglobin. Genetic mutations causing defects in the proper synthesis of hemoglobin result in various anemias. It is during the last phases of terminal erythroid differentiation that hemoglobin levels rise, making it a focus for therapeutic research. Fetal hemoglobin is comprised of ⍺-globin and gamma globin, then after a change in gene expression called hemoglobin switching which takes place after birth, adult hemoglobin is comprised of ⍺-globin and beta globin. We investigated hemoglobin switching and erythroid terminal differentiation by focusing on the master erythroid transcription factor Erythroid Krüppel-like Factor (EKLF). Data led …

The Roleo F Selectionin Maintaining Mhc Variationi N Mammals: Heterozygotaed Vantage,R Area Llelea Dvantagea Nd Mate Choice, Carol Titterton Ms

Student Scholarship

The major histocompatibility complex (MHC) is the most polymorphic gene

region in jawed vertebrates. Its gene products play a critical role in determining

individual and population fitness by presenting foreign antigens to immune system

cells, thereby initiating immune response to pathogen and parasite infections and

cancer. I surveyed the literature to investigate how selection maintains such

important adaptive diversity in mammalian populations and how MHC diversity

affects individual and population fitness. Balancing selection in the form of either

heterozygote advantage or rare allele advantage has long been proposed as the key

mechanism for maintaining the extreme diversity in MHC gene …

Bird Nesting Trends In Massachusetts, Dieter Rodriguez

Biology Student Scholarship

Bird nesting data has long been documented for various species across the United States. While trends between temperature changes or precipitation on bird nesting have been studied for particular species, studies done on the effects of temperature due to climate change on bird nesting on the broader state level are lacking. Using bird nesting data and average annual temperature data for Massachusetts collected over a ten year period, I assessed the effects of temperature changes over time on bird nesting attempts and total eggs laid for all documented species within the state. BIO 340 final project

Climate Effects On Butterfly Species Richness, Isabelle Heron

Biology Student Scholarship

The effects of climate change on the surrounding biodiversity lead scientists to argue an “insect apocalypse” is approaching. Butterflies, as poikilothermic animals, meaning their activity is closely controlled by weather, represent a useful indicator to studying climate change. Scientists can detect changes in butterfly abundance which result in changes in distribution and abundance over a short period of time. BIO 340 final project

Recent Advances In Bone Research 2022 Edition, Jean-Philippe Berteau, Laurent Pujo-Menjouet

Publications and Research

More and more scientific and engineering applications in bone research make pivotal advances in treating patients with orthopedics issues. Hence, bone research in the 21st century combines, inter alia, biology, chemistry, mathematics, and mechanics with complementary characteristics that help a holistic approach to bone-related pathologies. Nowadays, it is hard to connect new evidence when jargoning and money remain two significant obstacles to sharing knowledge. “Recent Advances In Bone Research” is a free book – no money involved at any stage - that combines the most recent efforts in bone research from several experts with different backgrounds, every expert seeks to …