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Host Responses To The Phenolic-Glycolipid-1 Antigen Of Mycobacterium Leprae (Elisa, Leprosy, Epidemiology, Armadillo, Human)., Richard W. Truman

LSU Historical Dissertations and Theses

Antibody responses to the apparently species specific phenolic-glycolipid-1 (Phen-Gl-1) antigen of Mycobacterium leprae were examined in humans and armadillos using enzyme-linked immunosorbent assays (ELISA). Statistical definitions for the interpretation of positive and negative reactions were derived. A retrospective serological survey of armadillos indicated that leprosy in the wild armadillo is a naturally acquired zoonosis. Presently 12.5% of the armadillos in 2 parishes in south central Louisiana have detectable IgM antibodies to Phen-Gl-1. Approximately 2.7% of these histologically exhibit clinical disease. Antibodies were not detected in Florida armadillo sera. Variations in prevalence rates were noted, and may be due to environmental …

Metabolic Responses Of The Estuarine Gastropod Thais Haemastoma To Hypoxia (Energy Charge, Opine Dehydrogenase,Survival, Adaptation, Respiration)., Martin A. Kapper

LSU Historical Dissertations and Theses

The hypoxia tolerance (LC-50) of Thais haemastoma was inversely related to acclimation temperature but was not related to acclimation salinity between 10 and 30(DEGREES)C and 10 and 30('o)/ooS. T. haemastoma is moderately tolerant to anoxia, the LT-50 (time to 50% mortality) being 12-22 days. Oxyregulatory ability varies from poor to good, and varies inversely with temperature. The oxygen consumption rate was lower after 28 days exposure to hypoxic conditions than at full oxygen saturation at all salinities at 20 and 30(DEGREES)C. Oxygen consumption was low at 10(DEGREES)C regardless of ambient PO(,2). Oxyregulatory ability in T. haemastoma did not show acclimation …

Epidemiology And Economic Implications Of Anaplasmosis In Louisiana (Prevalence, Incidence)., Randall Scott Morley

LSU Historical Dissertations and Theses

The research consisted of a literature review of the epidemiology of anaplasmosis and a descriptive epidemiological study of anaplasmosis in the Red River Plains and Southeast areas of Louisiana. The prevalence of anaplasmosis assessed by indirect fluorescent antibody (IFA) testing of 11,085 serum samples that had been obtained from cattle located in the study areas was 7.8%. Beef cattle appeared to show a higher Anaplasma antibody prevalence than dairy cattle, that is 11.2% to 3.8%, respectively. Among breeds, the Angus and Angus-cross animals seemed to show an increased susceptibility to infection. The prevalence of infection in free-roaming deer was determined …

Characterization Of The Modified Phagocytic Vacuole Occupied By Intracellular Toxoplasma Gondii (Macrophage, Protozoa)., L. David Sibley

LSU Historical Dissertations and Theses

Toxoplasma gondii is a protozoan parasite which resides in modified endocytic compartments of host cells. Extensive intracellular replication and host cell lysis leads to acute toxoplasmosis which is eventually limited by the host immune response to a chronic state of infection. The features of this unique intracellular compartment, which enables Toxoplasma to survive in macrophages, are reported here. Although Toxoplasma survives in normal macrophages, activated macrophages from immune animals rapidly killed Toxoplasma by production of oxygen radicals and intermediates generated during parasite invasion. In addition, activated macrophages inhibited Toxoplasma growth by an oxygen-independent mechanism. Qualitative and quantitative features of oxygen …

Production And Nitrogen Dynamics Of A Vallisneria Americana Grass Bed In Lake Pontchartrain, Louisiana (Nitrogen Cycle, Modeling, Seagrasses, Decomposition Phenology, Vallisneria-Celery Grass-Eelgrass, Environmental Factors)., Diana Lee Steller

LSU Historical Dissertations and Theses

Vallisneria americana grass beds of Lake Pontchartrain contribute approximately 200 g dry weight/m('2)/yr primary production, a continuous release of detrital material, and rapid nutrient recycling. A harvest method was used to estimate production. The addition of litterfall to harvest production raised annual production estimates by up to 28%. When litterfall was corrected for decomposition, the production estimates were raised by up to 85% over harvest estimates alone. Litterfall and decomposition increased toward shore and occurred throughout the year providing a continuous supply of energy and nutrients. Grass bed production varied along the coastline and was greatest at mid-depths. Values of …

The Oxytrichina Jankowski, 1980: Morphology, Morphogenesis, And Systematics (Sporadotrichina, Ciliphora, Cyst), Donald Early Culberson

Doctoral Dissertations

The hypotrich suborder Oxytrichina Jankowski, 1980 is a poorly characterised, systematically ambiguous taxon. A lack of ultrastructural and developmental information on a variety of oxytrichine species resulted in the establishment of multiple and disparate systematic categorizations for these species. I chose 8 species for morphologic and developmental studies: Oxytricha fallax, Oxytricha quadrinucleata, Oxytricha similis, Stylonychia pustulata, Tachysoma sp., Gonostomum affine, Pleurotricha lanceolata, and Paraurostyla sp. I used light microscopy and SEM for the study of trophozoites and dividing cells, and TEM and SEM in my investigations of cysts. The major unifying developmental characteristic of the suborder is the production of …

Prevention Of Carcinogenesis And Cancer Cell Proliferation By An Inhibitor Of Polyamine Biosynthesis (Dfmo, Hela, Mammary, Tumor), Rakesh Minocha

Doctoral Dissertations

The induction of mammary cancer in female Sprague-Dawley rats by a single of 1-methyl-1-nitrosourea was used as an experimental model system for identifying an effective agent in the prevention of breast cancer. The polyamine concentrations in whole mammary gland or mammary epithelial cells remained unchanged in the post-initiation stages of mammary carcinogenesis prior to the appearance of detectable tumors. However, mammary tumors had much higher concentrations of polyamines as compared to normal mammary gland. The development of chemically-induced carcinomas in rats was dramatically suppressed by the provision of a 1% solution of D,L-(alpha)-difluoromethyl-ornithine (DFMO), an inhibitor of polyamine biosynthesis, in …

Changes In The Biochemical Composition Of Testes During Spermatogenesis In Asterias Vulgaris, With Emphasis On The Role Of Polyamines In Regulating Proliferation, Frank F. Smith

Doctoral Dissertations

Testes of Asterias vulgaris are potentially useful for investigating mechanisms regulating spermatogenic events because of their structural simplicity and annual spermatogenic cycle. Examination of major biochemical classes during the spermatogenic cycle provides a definition of the changing chemical microenvironment influencing germinal cells and also suggests temporal relationships among successive spermatogenic events. Testes from seastars collected throughout the year were homogenized and lyophilized and aliquots assayed for DNA, RNA, total protein, free amino acids, total lipids, glycogen, and other carbohydrates; spermatogenic stage was determined by examination of paraffin sections. The resulting data, expressed as (1) mg/g dry mass, (2) mg/mg DNA, …

Control, Dynamics, And Kinetics Of Gas Exchange At The Onset And Offset Of Treadmill Exercise In The Pony (Oxygen Deficit, Debt, Ventilatory, Isocapric Hyperprea, Blood)., Scotty Kline Powers

LSU Historical Dissertations and Theses

The purpose of experiment one was to develop and validate an open circuit technique (OCT) employing a mixing-chamber for measurement of gas exchange during non-steady-state exercise in humans and ponies. Serial measurements of oxygen uptake ((')VO(,2)) using human subjects and ponies were made during rest to exercise transitions using the OCT incorporating a time delay to match mixed expired gas concentrations with the appropriate ventilatory volume. Oxygen uptake calculated by the OCT did not differ significantly (P > 0.05) from (')VO(,2) obtained by Douglas bag procedures. These data demonstrate that an OCT analysis system is a sensitive means of (')VO(,2) measurement …

Evaluation Of Romanomermis Culicivorax As A Biocontrol Agent Of Larval Mosquitoes In Louisiana Ricelands (Mermithidae, Nematode, Psorophora Columbiae, Parasitism)., Todd Warner Walker

LSU Historical Dissertations and Theses

Bionomic data were collected to evaluate Romanomermis culicivorax as a biocontrol agent of larval mosquitoes in Louisiana ricelands. In laboratory studies, the ability of R. culicivorax preparasites to infect Psorophora columbiae larvae substantially decreased after hosts were approximately 28 hr old. Parasitism at typical Louisiana rice field temperatures of 26, 29, and 32 (+OR-) 0.5(DEGREES)C showed a significant linear decrease (P < 0.01) as the percentage of older larval instars increased at the times of exposure. These data emphasized the need for a synchronous field application of preparasites to challenge the rapidly developing early instars of Ps. columbiae. Based on those data, applications of postparasites to potential mosquito breeding habitats offer greater flexibility in larval mosquito control programs. A floating bioassay container, constructed of plastic pipe, 50 mesh screening, and 8 oz (0.24 liter) sample bottles, was developed to evaluate R. culicivorax postparasites in irrigated agricultural land. Applications of postparasites applied at dosage rates of 0.025 and 0.05 g/m('2) resulted in the establishment, recycling, and overwintering of R. culicivorax in a riceland habitat in southern Louisiana. Infection rates ranged from 0 to 54.1% during the 22 month study. There was no significant differences (P > 0.05) between dosage rates among the plots and parasitism of Ps. columbiae larvae held in the bioassay containers. Depth of oviposition by R. culicivorax was determined by using soil cores previously inoculated with postparasites. Romanomermis culicivorax was found to …

The Impact Of Individual Species And Complexes Of Pest Arthropod Species On Soybean Grown On Various Row Spacings (Insect Pest Complexes, Non-Conventional Row Spacing)., Jaime Yanes Jr

LSU Historical Dissertations and Theses

During 1982 and 1983, selective soil and foliar insecticides were applied to soybean, Glycine max (L.) Merrill, planted on narrow (51 cm) and wide (76 cm) row spacings to manipulate soybean insects. Individual species and complexes of arthropod species were manipulated in plots to determine the effects of subterranean and above-ground insects on soybean growth and yield potential. Selective insecticides used in a selective manner proved to be an effective tool for studying insect pest complexes. Sub-threshold levels of individual pest species, in combination, had a significant negative impact on yield. In addition, in 1982, the plots in which soybean …

The Behavioral Ecology Of The First Instar Southern Green Stink Bug, Nezara Viridula (L.) (Aggregation, Pheromone, Embryo, Egg, Nymph)., Jeffrey Alan Lockwood

LSU Historical Dissertations and Theses

Outer row embryos of the southern green stick bug, Nezara viridula (L.), orient toward the center of the egg mass; inner row embryos do not. Embryonic orientation was mediated by stimuli provided by the glue which holds the egg mass together. Physical and chemical orientation cues associated with the glue were present only on the inner surfaces of outer row eggs; other eggs received no such differential stimuli and therefore oriented as a function of the oviposition behavior of the female. Embryonic orientation assured that upon hatching, outer row nymphs received the stimulation necessary for arrestment and inclusion in the …

The Role Of The Renin-Angiotensin System In The Drinking Response To Isoproterenol, Marilyn Margaret Robinson

Digitized Theses

Beta-adrenergic drugs, such as isoproterenol, potently stimulate renin secretion and drinking. It is controversial, however, whether the renin-angiotensin system directly mediates the dipsogenic response.;Isoproterenol (0.1 mg/kg, S.C.) alone caused rats to drink. Low doses of captopril (0.1-1.0 mg/kg, S.C.) which inhibit angiotensin II synthesis in the circulation but not in the brain dose-dependently enhanced isoproterenol-induced drinking whereas higher doses of captopril (5.0-100 mg/kg, S.C.) which inhibit angiotensin II synthesis in the brain as well, caused a dose-dependent inhibition of the response. The highest doses of captopril, either 100 mg/kg, S.C. or 20 mg/h, I.V., completely blocked the drinking response to …

Arterial Baro- And Chemoreceptor Projections To The Amygdala In The Cat, David Floyd Cechetto

Digitized Theses

In this study baro- and chemoreceptor input to the amygdala was investigated with electrophysiological experiments in chloralose-anesthetized cats and with the neuroanatomical tracing method of retrograde transport of horseradish peroxidase.;Stimulation of the carotid sinus (CSN) and aortic depressor (ADN) nerves altered the firing frequency of 30% (73/241) and 20% (50/251), respectively, of single units in the central, lateral and basal nuclei of the amygdala.;Selective activation of carotid baro- and chemoreceptors altered the firing frequency of 23% (35/154) and 16% (24/154), respectively, of amygdalar units, with chemoreceptor responsive units located primarily in the dorsomedial amygdala and baroreceptor responsive units located primarily …

Murine Hepatitis Virus (Mhv) Replication: A Molecular Study, Stephen Benet Cheley

Digitized Theses

L-2 cells infected with murine coronavirus (MHV) strains MHV-3 and JHM shut off host cell protein synthesis and synthesized polypeptides with apparent molecular weights 180,000 (p180), 56,000 (p56), 24,000, (p24) and 22,000 (p22) after a 15 minute pulse with {lcub}('35)S{rcub}-methionine. During a 2 hour chase period performed late in infection (5.5 h PI) a fifth polypeptide of molecular weight 50,000 (p50) was detected. The corresponding p56 and p50 polypeptides of the two viruses were enriched in the basic amino acid arginine. Pulse-chase and peptide mapping experiments confirmed a precursor-product relationship between the p56 and p50 proteins. MHV-3 virions, however, showed …

Biosynthesis Of Peroxidase In Peanut Cells In Suspension Culture, Ravindra Nath Chibbar

Digitized Theses

Peroxidase (E.C.1.11.1.7) has been widely used as a marker of altered growth and development in plants. However, a definitive role of physiological significance has yet to be ascribed. This may be partially due to studies, that have been performed with a peroxidase molecule neither isolated to purity nor characterized from the system of investigation. A cationic peroxidase molecule (molecular weight 40 kD) had been purified to apparent homogenity and characterized from cultured peanut cells. Antibodies were raised against this peroxidase molecule in rabbits and used for monitoring biosynthetic studies on this peroxidase molecule in cultured peanut cells.;The cationic peroxidase constituted …

The Bush Tick, R B. Besier

Journal of the Department of Agriculture, Western Australia, Series 4

A newly discovered livestock parasite has focused attention on farms in the Walpole district in the south-west of Western Australia. Ticks submitted to the Department of Agriculture in December 983 were identified as the bush tick, Haemaphysalis longicornis. An intensive campaign to locate areas of infestation and to limit spread was started in January 1984.

The bush tick is a pest of livestock in some areas of New South Wales and Queensland. It is important in a narrow coastal strip from Sydney to Brisbane where total summer rainfall can exceed 1000 mm. In this area heavy tick infestation causes …

G84-717 Fleas And Their Management (Revised January 1993), Shripat T. Kamble, David L. Keith

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide provides information on flea biology and how to solve flea problems.

Fleas are among the most annoying pests known to humans. In ancient times, fleas were even indicted, found guilty and sentenced to condemnation. Today, the verdict remains the same.

In Nebraska, fleas are common household pests attacking dogs, cats and sometimes people. In addition to these, several other flea species occur in Nebraska, feeding principally on rodents and occasionally on poultry and livestock. The information provided in this publication will focus primarily on cat and dog fleas, as they are the most commonly encountered species.

Interactions Of Soybean Arthropods And Crop Weeds: Diversity, Moth Nutrition, And Stability (Foraging Behavior, Nectar Analysis, Lepidopterous)., Fred Lewis Collins

LSU Historical Dissertations and Theses

Weeds which occur in crop ecosystems may cause suppressive or stimulating effects on arthropod abundance. Yet, little is known about the weed-insect interactions in Louisiana soybean. A three-part study was conducted from 1980-1983 to measure the abundance of arthropods in soybean, Glycine max Merr., as affected by the presence of absence of various weeds. A survey of arthropod abundance was conducted in commercial soybean fields which were either weedy or weed-free in central and south central Louisiana during 1980. Weedy fields had greater abundance of spiders, Geocoris punctipes (Say), and Lebia analis Dejean. Late instar green cloverworm, Anticarsia gemmatalis Hubner, …

Oviposition Of Psorophora Columbiae (Dyar And Knab) In Louisiana Pastureland., Dwight Charles Williams

LSU Historical Dissertations and Theses

Cattle hoofprint samples from a permanent pasture in Vermilion Parish, Louisiana during 1980 yielded an average of 9.2 eggs and capsules of Psorophora columbiae (Dyar and Knab)/hoofprint. Pasture sections differed significantly (P < .0001) in number of eggs and capsules/hoofprint. Sections ranged from 2.7 to 20.6 eggs and capsules/hoofprint. The number of eggs and capsules collected/date also differed significantly (P < .01), but peaks in numbers did not always correspond with peaks in rainfall or adult mosquito collections. Hoofprint densities and herd movement did not influence egg distribution. Estimates of numbers of eggs and capsules on the 30 ha permanent pasture range from 2,633,621 in late February to 16,913,025 on June 2, 1980. Mosquito oviposition was compared in hoofprint samples taken from a permanent pasture and a fallow rice field in Vermilion Parish, Louisiana during 1981. Hoofprints from the permanent pasture contained significantly (P < .001) more Ps. columbiae eggs than did hoofprints from the fallow rice field, averaging 3.3 and 1.9 eggs/hoofprint respectively. However, estimates of egg density in the 2 sites did not differ significantly (P > .05) since hoofprint density was greater in the fallow rice field. Egg density estimates were 15.2/m('2) for the fallow rice field and 13.3/m('2) for the permanent pasture. Hoofprint depth, hoofprint location, and cattle movement did not influence egg distribution. Results of studies with caged Ps. columbiae exposed to natural lighting conditions indicated that the oviposition cycle was bimodal with the main peak occurring in the 2 h period …

Electrophysiological Investigation Of Amygdaloid Inputs To The Ventral Pallidum Via The Nucleus Accumbens And Their Modulation By Dopamine, Conrad Chi Yim

Digitized Theses

The nucleus accumbens (NA) receives inputs from limbic structures and projects to the basal ganglia. In addition, it is the target of a heavy dopaminergic projection from the ventral tegmental area (VTA). The purpose of the present study is to investigate using electrophysiological techniques whether or not the NA forms a functional link between the amygdala (AMY) and the ventral pallidum (VP), and whether or not the mesolimbic dopamine projection from the VTA interacts with this pathway.;Electrical stimulation of the basolateral nucleus of the AMY caused an excitation was attenuated by ionotophoretically applied dopamine (DA) or electrical stimulation of the …

Hypothalamic And Peripheral Noradrenergic Responses To Changes In Baroreceptor Input In Conscious Rats With And Without Renal Nerves, Kaushik Parbhubhai Patel

Digitized Theses

Hypothalamic and peripheral noradrenergic systems have been implicated in baroreflex control of arteria pressure. However, the relationship between changes in the hypothalamic noradrenergic activity and changes in peripheral sympathetic activity is unclear. The first study examined this relationship in conscious Wistar rats following changes in baroreceptor input to the CNS produced by: (1) transection of aortic depressor nerve (ADN) (removal of specific baroreceptor input), (2) acute hypotension for 60 min produced by infusion of nitroprusside, and (3) acute hypertension for 60 min produced by infusion of phenylephrine. An index of norepinephrine (NE) turnover was used to assess the noradrenergic activity …

Superovulation And Infertility In Immature Rats, Elizabeth Anne Walton

Digitized Theses

This study examined the possible causes of infertility in immature rats following superovulation (SOV) with pregnant mares' serum gonadotrophin (PMSG). The areas looked at included the timing of ovulation, oocyte normality, fertilization, oviductal transport, early embryo development and implantation.;SOV with a single injection of PMSG or continuous infusion of follicle-stimulating hormone (FSH) was compared and in some rats the period of action of PMSG was restricted to (TURN) 58 h by use of a specific antiserum (a/s). Pregnancy was observed in most FSH-infused rats whereas following SOV with PMSG pregnancy failed prior to day 5. Increased ovarian oestradiol secretion was …

Androgen Regulation Of Fsh-Induced Aromatase Activity In Cultured Rat Granulosa Cells, Susan Anne Daniel

Digitized Theses

This study examines the role of androgen in the regulation of FSH-induced aromatase activity in rat granulosa cells in vitro. Experiments were designed to determine whether androgens act solely as substrates for FSH-induced aromatase activity or whether they also participate in the mechanism by which FSH increases the activity of the enzyme.;Granulosa cells were isolated from ovaries of immature rats and cultured during a 36- to 60-h "induction period" in the presence or absence of FSH with or without steroids and/or other compounds. Cells were then incubated for a further 6- and 12-h "test period" with aromatizable substrate (testosterone). Accumulation …

The Regulation Of Gene Expression During The Aerobic Germination Of Mucor Racemosus Sporangiospores., John Edgar Linz

LSU Historical Dissertations and Theses

This study was undertaken to investigate the regulation of gene expression during the aerobic germination of Mucor racemosus sporangiospores. Sucrose density gradient analysis of spore extracts revealed that the percentage of ribosomes associated with mRNA increased from 23% in dormant spores to 85% after 10 min of germination. L-{('14)C}leucine was immediately incorporated at a rapid rate into protein of a leucine auxotroph, whereas {('3)H}uracil or {('32)P}-phosphate were not incorporated into RNA at a significant rate until 20 min after the addition of medium. Newly synthesized RNA did not appear in polysomes until at least 30 min. Polyadenylated RNA, comprising 3.3% …

Evidence For An Outer Membrane - Association Precursor Of The Extracellular Protease Produced By Serratia Marcescens., Laura Robin Liss

LSU Historical Dissertations and Theses

Serratia marcescens LRL781, a gram-negative bacterium, produces an extracellular protease. Evidence is provided for an outer membrane-associated, larger molecular weight, enzymatically inactive precursor of the protease. Antiserum was prepared against the extracellular protease, which was purified from the supernatant fraction of S. marcescens LRL781 cultures. Column adsorption of the antiprotease serum was performed using a slurry of live cells and cellulose. The resulting effluent was serologically inactive against protease preparations and chromium chloride-coupled protease-rabbit erythrocytes. Whole washed cells agglutinated in the presence of the antiserum. Cells were uniformly labeled with ferritin conjugated goat anti-rabbit IgG, indicating that the protease precursor …

Sodium Ion Transport Regulation By A Serotonin Stimulated Adenylate Cyclase System In Freshwater Mussels., John Iver Scheide

LSU Historical Dissertations and Theses

Freshwater bivalves increase Na influx when the mussels were injected with dibutyryl cAMP, serotonin (5-HT), or 5-HT (10('-4) M/l) was added to the bath; a response similar to a sodium depleted mussel. Serotonin stimulated Na influx kinetics (K(,m) = 0.09 mM/l and J(,max) = 4.33 (mu)eq (g dry tissue (.) hr)('-1) were similar to Na depleted mussels. Stimulated Na influx (by dibutyryl cAMP or 5-HT) was inhibited by amiloride (5 x 10('-4) M/l) and the amiloride inhibition was reversible. Serotonin also changes water balance; the 5-HT stimulated mussels gain 0.36 ml H(,2)O (g dry tissue (.) hr)('-1) above controls. Serotonin …

The Effects Of Two Twelve-Week Training Programs On Sequential Measurements Of Blood Lipids, Hormones, Cardiovascular Function, Body Composition, And Strength In Sedentary Middle-Aged Males., Daniel Lee Blessing

LSU Historical Dissertations and Theses

A twelve-week study was conducted to determine the effects of weight training and jogging on serum lipids and hormones, cardiovascular function, body composition, and strength. Thirty-three subjects (mean age 45 + 6 years) comprised a weight training group (N = 9), running group (N = 11), and a control group (N = 13). All subjects exercised 3 days per week for 12 weeks. Statistical comparisons were completed by using a 3 x 3 ANOVA with repeated measures on time. Single Degree of Freedom Comparisons were used to examine the specific hypotheses about the differential effects across time among groups. The …

Relationship Between Gene Frequencies And Indices Of Physiological Stress In The Estuarine Gastropod, Thais Haemastoma., David Wendell Garton

LSU Historical Dissertations and Theses

The relationship between individual energy budgets and genetic variability was examined in Thais haemastoma acclimated to 7.5, 20 and 35('o)/oo S at 21(DEGREES)C. Energy budgets, after the equations of Crisp (1971), were determined by measuring rates of caloric ingestion, absorption efficiencies, oxygen consumption and ammonia and primary amine excretion. Genotypes at six polymorphic loci coding for soluble enzymes, of 25 total loci assayed, were determined using starch gel electrophoresis. The polymorphic loci examined were those encoding for (alpha)-L-amino peptidase (LAP), phosphoglucomutase (PGM), glucose-6-phosophate dehydrogenase (G6PDH), a non-specific esterase (Est) and two dipeptidases (L-leucyl-L-proline and L-phenylalanyl-L-proline dipeptidases). The genetic variability observed …

The Effects Of Acute Dehydration On The Anaerobic Threshold During Incremental Cycle Ergometer Exercise., Penelope England

LSU Historical Dissertations and Theses

Much of the previous research concerning dehydration and performance has centered around prolonged submaximal work while seemingly little attention has been focused upon short term incremental exercise. Numerous investigators have suggested that dehydration may alter carbohydrate metabolism in muscle. Theoretically, altered glycolytic flux in exercising muscle could result in a change in the rate of blood lactate (La) accumulation, and thus shift the anaerobic threshold. Anaerobic threshold (AT(,La)) has been formally defined as the oxygen uptake (V(,O(,2))) at which there is a systematic increase in blood La. Additionally, the AT(,La) has been estimated from a nonlinear increase in expired ventilation …