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A Seasonal And Ontogenetic Examination Of The Reproductive Biology Of Pseudoplusia Includens (Walker)., Linda Jean Mason

LSU Historical Dissertations and Theses

Laboratory observations on the mating behavior of soybean loopers, Pseudoplusia includens (Walker), indicated a calling pattern that was highly variable both within individual females over time and among individuals. However, some trends were apparent. The onset of calling showed temporal advancement over the first three nights of calling after adult emergence; the duration of calling showed a temporal advancement during the first six nights, while the termination of calling receded over the same period then reversed its trend. The majority of females initiated calling three nights following emergence while maximum percent calling was observed around five hours after the onset …

Bovine Enteropathogenic Coronavirus: The Effect Of The Host Cell And Trypsin Modification On The Virus Structure, Cytopathic Expression, And Infectivity., Karen P. St. Cyr-Coats

LSU Historical Dissertations and Theses

Human rectal tumor-18 (HRT-18) cell clones 3F3, 3E3, D2, and 4B3 exhibited differences in cellular morphology in Giemsa-stained cultures and developing monolayers. Differences were evident in growth kinetics and plating efficiency of each clone. The clones produced colonies in soft agar, demonstrating anchorage independence. Cytopathic expression (CPE) including cytoplasmic vacuolization and cell fusion occurred in BCV-L9-infected clones 3F3, D2, and 3E3. Cell fusion was inapparent in clone 4B3. Bovine coronavirus strain L9 (BCV-L9) and 5 wild-type isolates replicated in HRT-18 cells, inducing cell fusion. Strain L9, exclusively, replicated in D2BFS cells, requiring trypsin to induce cell fusion. Strain L9 produced …

An Investigation Of The Chlorophylls Of Selected Prasinophyte Algae., Leslie Carlat Kwasnieski

Student Work

Introduction: Chlorophyll a is the prevalent light-collecting pigment in eukaryotic, photosynthetic organisms. Several different chlorophylls exist in nature, but all autotrophic plants contain chlorophyll a (Bogorad, 1976). The majority of these chlorophyll a molecules function to absorb light and channel the excitation energy to photochemical sites in reaction centers. Chlorophylls b, c, d, and e, carotenoid pigments and phycobiliproteins are organized into antenna systems which absorb light maximally at different wavelengths. This design permits the visible light spectrum to be exploited (Foyer, 1984) for energy by different photosynthetic organisms. Chlorophyll b is present in higher plants, Chlorophyta, Prasinophyceae, and Euglenophyta; …

Quantifying Spatial Patterns Of Overgrowth In Epibenthic Communities, David S. Wethey, Linda J. Walters

Faculty Publications

A method is presented that allows rapid and accurate measurement of changes in the spatial pattern of space occupation in encrusting organisms. The boundaries of organisms are represented as polygons, and geometric difference calculations are used to establish the perimeters of the areas gained or vacated between sampling dates. The influence of interspecific interactions on occupation of space is also quantified: geometric intersection calculations are used to define the perimeters of the areas gained or lost in overgrowth interactions. A method is presented for estimating the sampling frequency necessary for detection of significant changes in occupation of space. The method …

Control Of Human Limb Movement Trajectories, Warren Garfield Darling

Digitized Theses

It is common experience that movements we make do not end at the same position during repeated attempts. Variability must, however, develop during movements and not just at their endpoint. Recent studies of the effects of perturbations on limb movements have suggested that the entire trajectory of movements, rather than their endpoint, may be controlled. Experiments were carried out to further examine this hypothesis by studying the variability in human arm movement trajectories and related muscle electromyographic (EMG) activities as a function of movement speed and practice.;Variability in both movements and the EMGs of involved muscles depended on both movement …

The Effects Of Estrogen, Progesterone And Ionized Calcium On Seizures During The Menstrual Cycle Of Mature Female Epileptics, John Jacono

Digitized Theses

More males than females are affected by epilepsy. This is so for all ages, with the exception of puberty. During the pubertal years, incidence rates of epilepsy are higher in females than in males.;Factors suspected of causing epilepsy are numerous, however the role of the female sex hormone estrogen is thought to be highly significant to epilepsy of puberty.;This study was designed to provide information about the role of estrogen and ionized calcium in seizures during the menstrual cycle of gynaecologically mature female epileptics. The study sample comprised 16 female epileptics ages 18 to 40 years resident in southwestern Ontario. …

Control Of Movement Initiation In Humans, Susan Holly Brown

Digitized Theses

The purpose of this study was three-fold: (1) does agonist activity (Ag1) duration remain constant over a wide range of movement amplitudes? (2) can Ag1 be modified by peripheral feedback? and (3) what is the precise relationship between Ag1 and the acceleratory phase of the movement?;In the first set of experiments, subjects performed 5 to 50 degree step-tracking movements about the elbow. In all subjects, small movements (5-20 deg) were initiated by short duration Ag1 bursts (70-80 ms) while large movements (40-50 deg) were initiated by long duration bursts (140-150 ms). The increased Ag1 duration resulted from a second peak …

Characteristics And Control Of Bilateral Arm Movement In Humans, Dhia Abdel-Hadi Al-Senawi

Digitized Theses

This study deals with the following questions: (1) How precisely are movement properties matched for the two arms? (2) Is matching of movements of the two arms affected by visual information? (3) Are movements of one arm affected by kinesthetic information arising from the contralateral arm? (4) Does alteration in the motor performance of one arm affect movement performance in the other arm?;During experiments, subjects grasped a vertical rod attached to a horizontal manipulandum bar. The subject's shoulder was abducted to 90 deg. The manipulandum bar was pivoted above the subject's elbow. Subjects made step-tracking flexion/extension movements about the elbow. …

Variations In The Biennial Life History Strategy Among 15 Ruderal Species In An Abandoned Gravel Pit Near London, Ontario, Paul Frederick Threadgill

Digitized Theses

Fifteen biennial species co-existed in an abandoned gravel pit near London, Ontario. Investigations were performed to identify variations in their regeneration characteristics. Once identified, variations were compared to attempt to explain these species' co-existence in this habitat. Mean net seed production per plant, mean seed weight per species, and dispersal of diaspores by wind, water, and animals were determined for each species. Mean seed production per plant ranged from 119 to 22,425. Mean weight per seed per species ranged from 0.07mg to 13.03mg. Mean weight per seed varied throughout the growing season in each of thirteen species. Diaspores of eight …

Facilitative And Inhibitory Interactions Among Estuarine Meiobenthic Harpacticoid Copepods (Commensalism, Benthos, Louisiana)., George Thomas Chandler Ii

LSU Historical Dissertations and Theses

In Louisiana, USA, marshes, the harpacticoid copepods Scottolana canadensis and Pseudostenhelia wellsi are community co-dominants with similar lifestyles. Scottolana canadensis is a semi-sessile burrow-dweller capable of subsurface suspension and deposit feeding. Pseudostenhelia wellsi is a semi-sessile tube-building copepod that continuously corkscrews back and forth within its tube and appears to graze on the inner tube walls. Monospecific patches of both species (250 (.) 5cm('-2)) were generated in laboratory microcosms to determine their effects on colonization by two completely errant, burrowing harpacticoids, Nitocra lacustris and Cletocamptus deitersi, that have similar foraging and burrowing behaviors and similar effects on sediment structure. Pseudostenhelia …

Effect Of Experimental Ostertagia Ostertagi Infection Or Chronic Pentagastrin Treatment On Abomasal Pathophysiology And Morphology Of Goats., Judit Eva Markovits

LSU Historical Dissertations and Theses

European-type mixed-breed goat kids were infected with Ostertagia ostertagi or were given chronic pentagastrin treatment. Selected clinicopathological parameters and fecal egg counts were monitored throughout the study. At necropsy the abomasal physical parameters were recorded and tissue samples were saved for parasite recovery, quantitative and qualitative light microscopy and transmission and scanning electron microscopy. Serum pepsinogen and gastrin immunoreactivity increased in parasitized animals. The increase was associated with the tissue phase of the parasitic development. Pentagastrin did not alter pepsinogen or gastrin immunoreactivity. All 8 infected animals had patent infection, and approximately 5% establishment of the infective dose of 15,000 …

Equine Herpesviruses Type 1 And 2: Growth And Cytopathic Expression In Organ Cultures Of Equine Respiratory Epithelium And In Cell Cultures (Electron Microscopy, Morphogenesis)., Emmanuel Eddie Brako

LSU Historical Dissertations and Theses

The pathogenesis of equine herpesvirus type 1 (EHV-1) and type 2 (EHV-2) respiratory infection was studied in cell and organ cultures by infectivity assays following experimental infections (growth kinetics), and by light, scanning and transmission electron microscopy. The cell cultures included fetal donkey and pony alveolar macrophages (FDAM/FPAM), fetal equine kidney (FEK), rabbit kidney (LLC-RK(,1)), and african green monkey kidney (VERO) cells. Organ cultures were derived from respiratory tissues (nasal, tracheal and pharyngeal mucosae) of seronegative donkey (F1D1) and pony (F1M1) fetuses, and of a seropositive adult pony (A1). In the cell cultures, EHV-1 multiplied and produced cytopathic effects (CPE) …

Circadian Lipogenic Responses To Insulin And Prolactin., Anthony Henry Cincotta

LSU Historical Dissertations and Theses

Lipogenic responsiveness to insulin was investigated at different times of day in freshly isolated hepatocytes and in primary cultured hepatocytes of Syrian hamsters, Mesocricetus auratus held on 14 hour daily photoperiods (lights on: 08.00) by following (('14)C)acetate incorporation into total cell lipid. The influence of prolactin on lipogenesis was tested by adding the hormone in vivo and directly to primary cultured hepatocytes. The findings of these studies are unique in that they demonstrate important roles of temporal interaction between prolactin and insulin in regulation of lipogenesis in cultured hepatocytes. They are summarized as follows: (1) A circadian variation of lipogenesis …

Anti-Mosquito Behavior Of Adult And Juvenile White-Footed Mice (Peromyscus Leucopus) (Treehole Mosquito, Aedes Triseriatus, Epidemiology), Connie Isaacs

Doctoral Dissertations

Rodents seldom are identified as hosts for mosquitoes, based on serological analysis of bloodmeals. However, due to limited survey and lack of sensitivity in most bloodmeal analyses, host species can be misrepresented or undetected. Knowledge of behavior of a potential host species can complement results of bloodmeal analysis, because active vertebrates may prevent mosquito feeding and thus rarely serve as hosts. The objectives of this study were to characterize anti-mosquito behavior in the white-footed mouse (Peromyscus leucopus noveboracensis) and to examine effectiveness of such behavior in mice based on age, prior exposure to mosquitoes, and immediate environment.

I used wild-stock …

A Mouse Model Of Bovine Pneumonic Pasteurellosis: Dose Response, Temporal Evolution Of Lesions And Effects Of Arachidonic Acid Inhibitors (Indomethacin, Nordihydroguaiaretic Acid, Hydroxyurea)., Lanny Wade Pace

LSU Historical Dissertations and Theses

Outbred Swiss Webster mice were used as an experimental model for bovine pneumonic pasteurellosis. Using light microscopy and transmission electron microscopy to evaluate lesions, it was determined that intrabronchial inoculation of Pasteurella haemolytica serotype 1 induces dose dependent pulmonary lesions in the mouse. Five x 10('7) colony forming units was the optimum dose which induced lesions and allowed survival for at least 24 hours. Using the optimum dose of inoculum, lesions were evaluated at different time points to determine when neutrophil influx into the lung first occurred. Four hours post inoculation was the minimum time required for neutrophil influx to …

The Effects Of Heartworm Infection And Beta Blockade On Submaximal, Graded Exercise In Dogs., Patricia Louise Hopkins Price

LSU Historical Dissertations and Theses

Four dogs with canine heartworm disease (CHD) and four control dogs were tested during a 21 minute submaximal, incremental, graded treadmill test, under normal conditions and after 2 mg/kg propranolol, i.v. Arterial and venous blood gas and pH, heart rate, systemic blood pressure, hematocrit, rectal temperature, lactate, glucose, and electrolytes were measured. CHD dogs had lower sodium and P(,a)O(,2) levels. All dogs displayed progressive increments in heart rate, hematocrit, and potassium and small gains in rectal temperature during exercise. Mean arterial blood pressure (MABP) and arterial pH increased and remained elevated throughout exercise. P(,v)O(,2) and P(,a)CO(,2) decreased, but P(,a)O(,2) did …

Molecular Studies Of T-Lymphocytes From Bovine Leukemia Virus-Infected Cattle., Diana Lynn Williams

LSU Historical Dissertations and Theses

Highly specific monoclonal antibodies and microfluorimetry were used to determine the absolute number of B and T lymphocytes in the peripheral blood of bovine leukemia virus (BLV) infected cattle. The peripheral blood lymphocyte populations from BLV-infected cattle were elevated when compared to BLV-negative animals. The increase in the lymphocyte population in the aleukemic (AL) animals was due to an increase in T lymphocytes. In the PL animals the increase was due mainly to an increase of B lymphocytes but also to an increase of T lymphocytes. The PL animals showed a significantly higher number of surface immunoglobulin positive cells (sIg('+)) …

Characterization Of Messenger Ribonucleoprotein Particles In Dormant Sporangiospores Of The Fungus Mucor Racemosus (Messenger-Rna)., Charles Patrick Chapman

LSU Historical Dissertations and Theses

Extracts of sporangiospores of Mucor racemosus contained RNA that readily hybridized with ('3)H polyuridylic acid. Prior to germination, this RNA was in a form sedimenting at <80S. Within 10 minutes after initiating germination, most of this RNA sedimented with polyribosomes and 80S monoribosomes. Particulate material from spore extracts bound to oligo dT -cellulose at high ionic strength and was assumed to contain messenger ribonucleoprotein particles (mRNP's). A portion of the mRNP's was released from the column by lowering the ionic strength. Other portions were eluted stepwise in buffer containing 50% and 90% formamide and in 0.1-N NaOH. Identical elution patterns were observed whether monitoring incorporated ('31)P-orthophosphate or L- ('32)S methionine, absorbance at 280 nm, or hybridization of ('3)H polyuridylic acid. mRNP's from the first two fractions were analyzed. A bimodal population of particles was detected in sedimentation velocity and sedimentation equilibrium centrifugation. Particles eluted at low ionic strength demonstrated a sedimentation coefficient distribution of 20S-to-80S, with a mean of 55S. Particles eluted in formamide demonstrated a sedimentation coefficient distribution of 20S-to-60S, with a mean of 40S. Particles eluted at low ionic strength displayed two peaks in CsCl centrifugation, with bouyant densities of 1.37 gm/cc and 1.59 gm/cc. Particles eluted in formamide displayed a single peak with a buoyant density of 1.61 gm/cc. Particles eluted at low ionic strength and centrifuged in metrizamide solution formed two bands having bouyant densities of 1.15 gm/cc and 1.30 gm/cc; formamide-eluted particles banded only at the higher density. Mucor 40S ribosomal subunits banded at 1.56 gm/cc and 1.28 gm/cc in CsCl and metrizamide solution respectively. Analysis of mRNP fractions by sodium dodecylsulfate-polyacrylamide gel electrophoresis characterized protein components of these particles which helped explain their biomodal sedimentation behaviour. Each mRNP fraction contained a unique spectrum of proteins not shared by ribosomes or soluble cell proteins. The formamide-eluted particles contained two proteins, whereas the particles eluted at low ionic strength possessed 12 proteins. A 24,000-dalton protein was the predominant form in both. The denser mRNP's were smaller and lacked proteins found in the larger less dense particles. The former had a protein/RNA ratio of 45/55; whereas the later possessed a value of 90/10. Messenger RNA extracted from the mRNP's sedimented between 8S-to-20S.

Glyphosate Catabolism By Pseudomonas Sp. Pg2982., Dean Lee Shinabarger

LSU Historical Dissertations and Theses

The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing 3-('14)C glyphosate revealed that approximately 50-59% of the C3 carbon was oxidized to CO(,2). Fractionation of stationary phase cells labeled with 3-('14)C glyphosate revealed that from 45-47% of the assimilated C3 carbon is distributed to proteins and that the amino acids methionine and serine are highly labeled. The nucleic acid bases adenine and guanine received 90% of the C3 label that was incorporated into nucleic acids, and the only pyrimidine base labeled was thymine. These results indicated that …

Tolerance, Bioenergetics And Biochemical Composition Of The Blue Crab, Callinectes Sapidus Rathbun, Exposed To The Water-Soluble Fraction Of South Louisiana Crude Oil., Shiao Yu Wang

LSU Historical Dissertations and Theses

The tolerance, bioenergetics, growth, molting frequency and biochemical composition of juvenile blue crabs, Callinectes sapidus, exposed to the water-soluble fraction of South Louisiana crude oil were determined during a 21-d exposure period. Blue crabs were highly tolerant to petroleum hydrocarbons; the LC50 was 3927 ppb on day 21. Rates of energy intake decreased with increasing hydrocarbon concentration. Although energy expenditure rates of crabs exposed to 2504 ppb were not significantly higher than that of control crabs, they were significantly higher than those of crabs exposed to 820 and 1476 ppb. The resulting decrease in scope for growth was thus due …

The Hepatotoxicity And Urinary Metabolites Of 2,4- And 2,6-Dimethylaniline In The Rat And Dog (Aromatic Amines)., Marcia L. Hardy

LSU Historical Dissertations and Theses

This study was designed to investigate the hepatotoxicity and urinary metabolites of 2,4- and 2,6-DMA in the dog and rat and the covalent binding of the two isomers in the rat as differences in metabolism may be related to the divergent hepatic responses. Male Beagles were orally dosed for 1 or 10 days with 2,6- or 2,4-DMA at 25 mg/kg. Male Fischer 344 rats were gavaged with 2,6- or 2,4-DMA at 25% of their respective LD50's (262.5 mg/kg; 117/mg/kg) for the same time period. The effects of phenobarbital (PB), 3-methylcholanthrene (3MC) and SKF-525A on the hepatotoxicity and metabolites of 2,4- …

Microbial Degradation Of The Leachable And Lignocellulosic Components Of Leaves And Wood From Rhizophora Mangle In A Tropical Mangrove Swamp, Ronald Benner, Robert E. Hodson

Faculty Publications

Preparations of uniformly [¹⁴C] labeled mangrove leaves and specifically radiolabeled [¹⁴C-lignin] lignocelluloses and [¹⁴C -polysaccharide]lignocelluloses from mangrove leaves and wood were used in experiments to determine the microbial rates of mineralization of the leachable and lignocellulosic components of mangrove detritus in aerobic and anaerobic sediments of a tropical mangrove swamp. The bulk of the leachable fraction from mangrove leaves was mineralized relatively rapidly and was assimilated into microbial biomass with high efficiency (30 %). In contrast, rates of mineralization of the lignocellulosic component of mangrove leaves and wood were 10fold lower than mineralization rates …

Terrestrial Invertebrates Of The Virgin Islands, Wendy Jackelow

Theses

None provided.

Taylor University Magazine (Spring 1985), Taylor University

The Taylor Magazine (1963-Present)

The Spring 1985 edition of Taylor Magazine, published by Taylor University in Upland, Indiana.

Assay For 5-Methylcytosine In The Dna Of Dictyostelium Mucoroides At Two Different Stages In Its Life Cycle., Cynthia A. Rice

Student Work

Introduction: The Acrasiales, or cellular slime molds, are not true molds at all. They are a unique group of organisms that exhibit characteristics of both the fungi and the protozoa. The cellular slime molds are more than a taxonomic enigma, however. One genus, Dictyostelium, has been extensively studied as a model of multicellular development.

Stress Fibres In Cells From Normal And Dystrophic (Bio 146) Hamsters And Identification Of A Desmin-Like Protein From Hamster Muscle, Michael Andrew Pollock

Digitized Theses

The BIO 14.6 line of Syrian hamsters has been a useful model system for Duchenne dystrophy since its introduction (Homburger et al., 1962b). To reduce the effects of genetic background in this model system, a control line ('BC') was produced which shares >90% of its genotype with the BIO 14.6 line. These control animals did not differ from random-bred ('RB') control hamsters under the investigative techniques used here.;In this study, stress fibres in cultured fibroblast-like cells from muscle and skin were examined by direct staining and by indirect immunofluorescence using antibodies to the muscle proteins (alpha)-actinin, tropomyosin and M-line protein. …

Physiological Determinants Of Aerobic Function In Elderly Males, Scott Gordon Thomas

Digitized Theses

Low cardiorespiratory fitness in the elderly is due to aging and to inactivity. Factors which limit performance of aerobic exercise and which influence the response to physical training were examined in a sample (n = 224) of elderly males (age 62 yrs). Subjects were randomly assigned to a training or control group. Maximum oxygen uptake (VO2max), pulmonary function and body composition were determined at entry (T1) and one year later (T2). Exercise testing included a continuous, incremental test (Stage I) and a discontinuous test with three steady state, submaximal loads on a motor driven treadmill. Cardiac output was estimated with …

The Population Ecology And Nutrient Transport Of Gulf Menhaden In Fourleague Bay, Louisiana (Mortality, Growth, Feeding, Production, Migration)., Linda Ann Deegan

LSU Historical Dissertations and Theses

An ecological study of the young-of-the-year of three year-classes of gulf menhaden (Brevoortia patronus) was conducted from July 1981 to December 1983 in Fourleague Bay, Louisiana. This study documented gulf menhaden population ecology and the importance of gulf menhaden in the nutrient and energy balance of estuaries. Young-of-the-year gulf menhaden immigrated in winter and moved to tidal creeks and ponds, where they remained until midsummer. They then moved into open bays, where they remained until the fall. This migration pattern coincides with sequentially available, but spatially separate, productivity peaks in marsh and open bays. In the fall, they emigrated to …

Molecular Studies Of Thamnophiine Snakes., Robin Lawson

LSU Historical Dissertations and Theses

Variation in alleles at gene loci encoding proteins was used to determine zoogeographic and evolutionary differentiation in thamnophiine snakes. Differentiation among populations within a species, among species within a genus, and among genera was investigated. In the southern banded water snake, Nerodia fasciata, analysis by F-statistics and genetic distances of allele frequencies indicated that extensive differentiation has occurred between those forms adapted to a saltwater environment (N. f. clarkii and N. f. compressicauda) and those adapted to a freshwater environment (N. f. fasciata, N. f. confluens, and N. f. pictiventris). Habitat disturbance is imputed as the causal agent in the …

The Biology Of Egg Production In The House Cricket, Acheta Domesticus L., Craig William Clifford

LSU Historical Dissertations and Theses

Oogenesis commenced on day 3 (day 0 = adult ecdysis) in Acheta domesticus L., which, based on degeneration of the flight muscles, was approximately when juvenile hormone was released. Starvation did not prevent the onset of oogenesis, although egg production was greatly reduced. Virgin females did not oviposit and the oviducts filled with eggs by day 15. Compaction appeared to be a factor limiting oogenesis. The digestive tract was compressed and feeding reduced, which might be the primary factors terminating oogenesis. The concentration of juvenile hormone esterase (JHE) did not change as virgins filled with eggs, which indicated that JHE …