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Density And Aboriginal Uses Of Wild Tree Species In Milawilila Forest Reserve In Morogoro Region, Tanzania, David Sylvester Kacholi Mar 2020

Density And Aboriginal Uses Of Wild Tree Species In Milawilila Forest Reserve In Morogoro Region, Tanzania, David Sylvester Kacholi

Tanzania Journal of Science

This study assessed density and local uses of wild tree species in Milawilila forest reserve, Morogoro rural district. A total of six transects were set in the forest for assessing density and diversity of trees. In each transect, three plots of 20 m x 20 m were established and all trees with Diameter at Breast Height (DBH) ≥ 10 cm were identified, counted and DBH recorded in each plot. A semi structured questionnaire was used to obtain data on the use of tree species by the locals. Data were analyzed using Microsoft Excel and Statistical Package for Social Science software. …


Assessment Of The Phytotoxic Effects And Ecological Risks To Phaseolus Vulgaris Planted On Crude Oil Spiked Soils, Doris F. Ogeleka, Patrick C. Bokolo, Gloria O. Omoregie Mar 2020

Assessment Of The Phytotoxic Effects And Ecological Risks To Phaseolus Vulgaris Planted On Crude Oil Spiked Soils, Doris F. Ogeleka, Patrick C. Bokolo, Gloria O. Omoregie

Tanzania Journal of Science

Crude oil can contaminate environmental matrices during extraction, production, refining, loading and offloading. This study evaluated the phytotoxic effects and ecological risks to beans (Phaseolus vulgaris) planted on native soils spiked with concentrations of crude oil with a secondary aim to phytoremediate the soil. The results showed a decrease in plant heights (60.80 ± 2.11 to 25.30 ± 1.10 cm), leaf areas (40.00 ± 1.70 to 23.60 ± 1.40 cm2), leaf number (14.00 ± 0.00 to 8.00 ± 0.00), and stem girth (1.57 ± 0.06 to 1.33 ± 0.06 cm) with increasing crude oil concentrations. The total petroleum hydrocarbons (TPH) …


Spore Germination For Three Edible Zimbabwean Mushrooms Using Biostimulants, Melody Pamire, Wilson Parawira Mar 2020

Spore Germination For Three Edible Zimbabwean Mushrooms Using Biostimulants, Melody Pamire, Wilson Parawira

Tanzania Journal of Science

Spores play critical roles in the population and community development of ectomycorrhizal fungi (EMF), which grow naturally in the presence of host plants. EMFs have not been cultivated commercially in most countries including those in Africa. In vitro germination of their spores is refractory and has been witnessed only in the presence of stimulants. In this study, germination of spores for three wild ectomycorrhizal fungi commonly collected in Zimbabwe was investigated under the influence of three biostimulants in Murashige and Skoog (MS) and Potato Dextrose Agar (PDA) media for the first time. The stimulatory capacity of tomato or potato roots …


Epidemiological Significance Of Parasitic Infections In Olive Baboons (Papio Anubis) At Gombe National Park, Tanzania, Jared S. Bakuza Mar 2020

Epidemiological Significance Of Parasitic Infections In Olive Baboons (Papio Anubis) At Gombe National Park, Tanzania, Jared S. Bakuza

Tanzania Journal of Science

Several parasites of significant health importance have been observed in baboons at Gombe National Park in western Tanzania.  However, little is known on impacts these infections have on baboon health. Baboon stools were examined for parasites using formol-ethyl technique and their health and troop membership associated with parasitic infections. Observed nematode parasites were; Streptopharagus sp., Physaloptera sp., unidentified nematodes, hookworms and Trichuris sp. Trematode parasites included Schistosoma mansoni and unidentified trematode, with the latter’s intensity being highest in DB troop baboons. Parasite egg counts (epg) for nematodes ranged from 9 to 12, and that of trematodes from 4 to 40 …


Altitudinal Distribution And Monthly Occurrence Of Butterflies In The Kihansi Gorge Forest, Tanzania, With A Checklist Of Species, Devolent Mtui, Colin Congdon, Ivan Bampton, Parson Kalenga, Haule Leonard Dec 2019

Altitudinal Distribution And Monthly Occurrence Of Butterflies In The Kihansi Gorge Forest, Tanzania, With A Checklist Of Species, Devolent Mtui, Colin Congdon, Ivan Bampton, Parson Kalenga, Haule Leonard

Tanzania Journal of Science

The goal of this project was to establish a checklist of butterflies occurring in Kihansi gorge to serve as baseline for monitoring of gorge ecosystem health. The key questions were what and how many species of butterflies occur in the gorge with respect to time and space. The study was conducted between October 2005 and February 2007. Butterflies were recorded twice a week, along a walking trail, using malaise traps and sweep net. The traps were located at upper (> 750 – 1100 m), mid (> 580 – 750 m) and lower (300 – 580 m) gorge. A total of …


Reintroduction Of The Kihansi Spray Toad Nectophrynoides Asperginis Back To Its Natural Habitat By Using Acclimatizing Cages, Charles A. Msuya, Nassoro Mohamed Dec 2019

Reintroduction Of The Kihansi Spray Toad Nectophrynoides Asperginis Back To Its Natural Habitat By Using Acclimatizing Cages, Charles A. Msuya, Nassoro Mohamed

Tanzania Journal of Science

The Kihansi Spray Toad (Nectophrynoides asperginis) is considered to be extinct in the wild. Captive breeding populations exist in Bronx and Toledo zoos in USA and in two captive breeding facilities in Tanzania. Efforts to reintroduce the species back to its natural habitat at Kihansi Gorge wetlands have become a long process. Both ex-situ and in-situ experiments have revealed promising outcomes but when the toads are freely (hard) released in the wetlands they disperse widely and detection becomes difficult.  Cages for acclimatising the toads before hard release have been constructed in two of the Kihansi Gorge spray wetlands. Factors such …


Morphological Diversity Of Wild Coffee (Coffea Kihansiensis) A Potential Coffee Species For Genetic Improvement, Paul M Kusolwa, Faraja Makwinja, Jackson Nashon, Mayomba Marianna, Amina Kibola Dec 2019

Morphological Diversity Of Wild Coffee (Coffea Kihansiensis) A Potential Coffee Species For Genetic Improvement, Paul M Kusolwa, Faraja Makwinja, Jackson Nashon, Mayomba Marianna, Amina Kibola

Tanzania Journal of Science

Coffee belongs to the genus Coffea in the Rubiaceae family, and is mostly grown in the tropical and subtropical regions. The Coffea genus comprises 103 species, of which Coffea arabica L. (Arabica coffee) and Coffea canephora P. (Robusta coffee) are the two most important commercial species. C. Arabica is a commercially important high quality coffee with low genetic diversity, while C. canephora is cultivated mostly in the northwestern region of Tanzania. Of recent, a discovered wild coffee (Coffea kihansiensis) in Tanzania may be a genetic resource for improvement of cultivated coffee.  Morphological descriptors from accessions evaluated in different sites at …


Land Use–Land Cover Change And Implication To Biodiversity In Kihansi Catchment, Phillip K. Mwanukuzi Dec 2019

Land Use–Land Cover Change And Implication To Biodiversity In Kihansi Catchment, Phillip K. Mwanukuzi

Tanzania Journal of Science

Land cover-land use (LC-LU) change has significant impact on wetland ecosystem. This study examined the impacts of LC-LU change on spray wetland ecology at Kihansi Gorge and implications to conservation of fragile biodiversity. Remote sensing methods were used to asses land cover changes from 1990 to 2015 and to forecast change in 2035. It was revealed that forests decreased from 28,454 to 10,897 ha and woodland from 26,903 to 19,914 ha. In 1990, woody cover dominated with a total cover of 38% of the entire catchment. The cropland that included grassland and bushland with scattered crops has been on the …


Management And Population Status Of Kihansi Spray Toad Nectophrynoides Asperginis In Captive Breeding Facilities In Tanzania, Cuthbert L. Nahonyo, Ezekiel M. Goboro, Richard S. Ugomba, Emmanuel S. Nkombe, Severinus Mutagwaba, Hussein Adam, Juma I. Kimera, Person Kalenga, Wilirk Ngalason Dec 2019

Management And Population Status Of Kihansi Spray Toad Nectophrynoides Asperginis In Captive Breeding Facilities In Tanzania, Cuthbert L. Nahonyo, Ezekiel M. Goboro, Richard S. Ugomba, Emmanuel S. Nkombe, Severinus Mutagwaba, Hussein Adam, Juma I. Kimera, Person Kalenga, Wilirk Ngalason

Tanzania Journal of Science

Kihansi spray toad Nectophrynoides asperginis was discovered in 1996 in Kihansi gorge. The toad was declared extinct in the wild in 2009 due to habitat alteration. Before its extinction, 499 individuals were flown to the United States of America zoos in 2000 for captive breeding. In 2010, the University of Dar es Salaam, and in 2011 the Kihansi captive breeding facilities were established in Tanzania. The intention was to breed, and reintroduce the toad back to the natural environment in the gorge. The founder populations for the two facilities were sourced from the USA zoos. This study addresses captive management …


Some Histopathological Findings In Dead Kihansi Spray Toads In Captivity, Joshua J Malago Dec 2019

Some Histopathological Findings In Dead Kihansi Spray Toads In Captivity, Joshua J Malago

Tanzania Journal of Science

Raising of Kihansi spray toad (KST), Nectophrynoides asperginis, in captivity is associated with disorders that can be fatal. Since diagnosis of these disorders cannot be confirmed grossly, the current study was aimed at exploring histopathological findings in dead KSTs kept under captivity in Tanzania. Dead KSTs were immediately recovered, observed for gross changes, fixed in 10% neutral buffered formalin, processed routinely, and stained sections reviewed for histological changes. Observed infectious agents were strongyloides (25.3%), ciliates (1.7%), lungworms (0.6%), fungi (3.4%) and bacteria (5.1%) or mixed infections (9%) of these agents. Noted histopathological lesions in affected organs included infiltration of inflammatory …


Research Work And Conservation Initiatives In The Kihansi Catchment Ecosystem, Tanzania, Cl Nahonyo Dec 2019

Research Work And Conservation Initiatives In The Kihansi Catchment Ecosystem, Tanzania, Cl Nahonyo

Tanzania Journal of Science

Keynote Address  for the Scientific Conference on Research and Conservation Initiatives in the Kihansi Catchment Ecosystem: Successes, Challenges and Way forward held at the National Carbon Monitoring Centre (NCMC), Sokoine University of Agriculture (SUA) on 24 – 27 July 2017.


Histopathological Diagnosis Of Diseases Affecting Amphibians Inhabiting Kihansi Gorge, Tanzania, Joshua J Malago Dec 2019

Histopathological Diagnosis Of Diseases Affecting Amphibians Inhabiting Kihansi Gorge, Tanzania, Joshua J Malago

Tanzania Journal of Science

Kihansi gorge is a natural habitat to Kihansi spray toad (KST), Nectophrynoides asperginis. The toad is extinct in its natural habitat partly due to diseases. Efforts to successfully re-introduce it are primed by awareness and mitigation of prevalent diseases at the gorge. This study aimed at exploring the prevalent amphibian diseases at and around Kihansi gorge. A total of 182 individuals from Petropedetes yakusini (n = 60), Nectophrynoides tornieri (n = 60) and Leptopelis uluguruensis (n = 62) species were collected, humanely sacrificed, fixed in formalin, routinely processed, sectioned, stained with haematoxylin and eosin and analysed for diseases by histopathology. …


Radioactivity Assessment Of 40k, 238u And 232th In Surface Soil Samples Of Igbokoda, Southwest Of Nigeria, Olusegun Sowole, Kolawole A. Egunjobi Dec 2019

Radioactivity Assessment Of 40k, 238u And 232th In Surface Soil Samples Of Igbokoda, Southwest Of Nigeria, Olusegun Sowole, Kolawole A. Egunjobi

Tanzania Journal of Science

Natural radionuclides are found in soil and are capable of disintegrating leading to the release of ionizing radiations that can have harmful effects on individuals exposed to them most especially when exceeding the recommended global limits of radiological parameters. Assessment of activity  concentrations of 40K, 238U and 232Th in surface soil samples from different locations  at Igbokoda where crude oil exploration was taking place, at Ondo State in Nigeria, had been determined by gamma spectrometry using NaI (TI) detector coupled with a pre-amplifier base connected to a multiple channel analyzer (MCA) which was used to calculate the radiological indicators. 10 …


The Need Of A Structured Tilapia Breeding Program In Tanzania To Enhance Aquaculture Production: A Review, Redempta A. Kajungiro, Levinus L. Mapenzi, Christer S. Nyinondi, Anna Norman Haldén, Aviti J. Mmochi, Mwita Chacha, Matern S.P. Mtolera, Hieromin A. Lamtane, Dirk Jan De Koning Dec 2019

The Need Of A Structured Tilapia Breeding Program In Tanzania To Enhance Aquaculture Production: A Review, Redempta A. Kajungiro, Levinus L. Mapenzi, Christer S. Nyinondi, Anna Norman Haldén, Aviti J. Mmochi, Mwita Chacha, Matern S.P. Mtolera, Hieromin A. Lamtane, Dirk Jan De Koning

Tanzania Journal of Science

Breeding programs are crucial for boosting productivity and increase sustainability of aquaculture. Over years, Tanzania has witnessed fluctuation in its capture fisheries production from 320,900 to 375, 535 and back to 362,595 metric tonnes in the years 2000, 2005 and 2016, respectively (URT 2016). The declining trend in fish production has made fish supply in the country unstable and conversely, increased the demand for fishes to about 730,000 metric tonnes in 2017. However, the local aquaculture production has not increased accordingly. Tanzania is importing fish mainly from Asia to meet its increased demand. In 2017, a total of 2,055,721 kg …


Phytoremediation Potential Of Four Heavy Metals In Soil By Chromolaena Odorata (L.) King & Robinson At The Phytotoxicity Screening Benchmarks, Gloria O. Omoregie, Beckley Ikhajiagbe, Geoffery O. Anoliefo Dec 2019

Phytoremediation Potential Of Four Heavy Metals In Soil By Chromolaena Odorata (L.) King & Robinson At The Phytotoxicity Screening Benchmarks, Gloria O. Omoregie, Beckley Ikhajiagbe, Geoffery O. Anoliefo

Tanzania Journal of Science

The remediative capacity of Chromolaena odorata with respect to heavy metal induced growth-stress was investigated. This was with a view to providing information on the adaptive mechanisms adopted by the test plant during the phytoremediation of selected heavy metals such as copper, manganese, zinc and cadmium. Stems of C. odorata were planted in soils polluted with  Mn, Zn, Cd, and Cu at the ecological screening benchmark (ESB) of 50 mg/kg (Mn and Zn), 4 mg/kg (Cd) and 100 mg/kg (Cu) of metal per kilogram of soil, in varying concentrations of 0 ESB (control), 1 ESB, 3 ESB and 5 ESB. …


Characterization And Domestication Of Wild Edible Mushrooms From Selected Indigenous Forests In Burundi, Vincent Nteziryayo, Donatha D. Tibuhwa, Prosper Kiyuku, Tatien Masharabu Dec 2019

Characterization And Domestication Of Wild Edible Mushrooms From Selected Indigenous Forests In Burundi, Vincent Nteziryayo, Donatha D. Tibuhwa, Prosper Kiyuku, Tatien Masharabu

Tanzania Journal of Science

In Burundi, minimum work has been done to comprehensively identify and commercialize high yielding local mushrooms. The previous studies carried out on mushroom cultivation have focused on exotic strains. This is the first study undertaken on domestication of wild edible mushrooms from Burundi indigenous forests. Nine samples were collected from four protected areas and characterized using phenotypic and molecular markers. Germoplasm isolation through tissue culture techniques, spawn production and cultivation studies were also undertaken. Mushroom samples were identified as Pleurotus citrinopileatus, Lentinus squarrosulus, Hypholoma fasciculare, Laetiporus sulfureus, Macrolepiota dolichaula, Trametes polyzona, Amanita zambiana, Lactarius delicious and Amanita verna. Spawn production …


Antimicrobial Activities Of Endophytic Fungi Secondary Metabolites From Moringa Oleifera (Lam), Zuhura N. Mwanga, Esther F. Mvungi, Donatha D Tibuhwa Dec 2019

Antimicrobial Activities Of Endophytic Fungi Secondary Metabolites From Moringa Oleifera (Lam), Zuhura N. Mwanga, Esther F. Mvungi, Donatha D Tibuhwa

Tanzania Journal of Science

This study presents the antimicrobial activities of secondary metabolites of endophytic fungi isolated from a medicinal plant Moringa oleifera growing at the University of Dar es Salaam, Tanzania. Two endophytic fungi were identified, Nigrospora sp. from leaves, and Fusarium sp. from roots and seeds. The disc method was used in testing the antimicrobial activities of the crude extracts of endophytic fungi secondary metabolites against four human pathogens, namely, Bacillus substilis, Staphylococcus aureus, Escherichia coli, and Candida albicans. The results revealed that the crude extracts of endophytes secondary metabolites from leaves showed strong antimicrobial activities against two Gram positive bacteria and …


Effects Of Seasons And Zonation On Biochemical Contents Of Two Seagrass Species Along Dar Es Salaam Coast, Tanzania, Wamwi M. Mbaga, Esther F. Mvungi Dec 2019

Effects Of Seasons And Zonation On Biochemical Contents Of Two Seagrass Species Along Dar Es Salaam Coast, Tanzania, Wamwi M. Mbaga, Esther F. Mvungi

Tanzania Journal of Science

Accumulation and allocation of biochemicals in plants are affected by various factors. The influence of seasons and vertical zonation on biochemical contents of seagrasses Cymodocea rotundata and Thalassia hemprichii from Mjimwema seagrass meadow along the coast of Dar es Salaam were assessed. Samples were collected in 2016 during wet/rainy (April–May) and dry (June–July) seasons. Concentrations of crude protein, soluble carbohydrates, phosphorus, lipid, and proline were determined using standard methods. Significant higher levels of phosphorus, lipid, protein and proline were observed during the dry season than in wet season (p < 0.05). Likewise, seagrass population occurring at the shore zone expressed higher levels of biochemical contents than those found at the subtidal zones. The highest concentration of protein was recorded in Thalassia hemprichii (5.8 mg/g dw)), while those of lipid and carbohydrates (27.1 and 53.1 mg/g dw, respectively) were recorded in Cymodocea rotundata. The two species studied did not vary significantly in their biochemical concentrations in both seasons. Generally it was observed that the concentrations of studied biochemicals were up-regulated during the dry season and varied depending on the locations where the seagrass grow. Thus, both season and zonation played roles in biochemical accumulation in the seagrasses understudy, however not interactively.


Assessment Of Tree Species Richness, Diversity, Population Structure And Natural Regeneration In Nongeni Forest Reserve In Morogoro Region, Tanzania, David Sylvester Kacholi Dec 2019

Assessment Of Tree Species Richness, Diversity, Population Structure And Natural Regeneration In Nongeni Forest Reserve In Morogoro Region, Tanzania, David Sylvester Kacholi

Tanzania Journal of Science

This study examined tree species richness, diversity, population structure and regeneration in Nongeni forest reserve, Morogoro, Tanzania. The study deployed plot sampling technique whereby a total of 20 plots of 0.05 ha each were randomly established in the forest. All species and individuals encountered in each plot were counted, identified and DBH measured. A total of 751 individuals/ha representing 24 species belonging to 11 families were recorded. Family Fabaceae was dominant with 9 species. The Shannon-Wiener, Margalef, Simpson’s and Pielou J index were calculated as 2.667, 3.474, 10.58 and 0.839, respectively. The Diplorhynchus condylocarpon (52.18) was the dominant species in …


Kleptoparasitism Of Shoebills Balaeniceps Rex By African Fish Eagles Haliaeetus Vocifer In Western Tanzania, Jasson John, Woo Lee Aug 2019

Kleptoparasitism Of Shoebills Balaeniceps Rex By African Fish Eagles Haliaeetus Vocifer In Western Tanzania, Jasson John, Woo Lee

Tanzania Journal of Science

Kleptoparasitism is a specialised form of foraging interference occurring throughout the animal kingdom and especially is well documented among birds including African fish eagle Haliaeetus vocifer. However, only one record is reported between fish eagle and shoebill Balaeniceps rex despite their habitat overlap and it remains undescribed. This paper documents kleptoparasitism of shoebills by fish eagles in Malagarasi wetlands, western Tanzania. Kleptoparasitism was highly seasonal occurring in the dry season, especially at low floods. Both adults and non-adult eagles stole prey from shoebills, with non-adults initiating attacks at shorter distances than adults perhaps because of the lack of experience. The …


Species Composition, Diversity And Distribution Of Vascular Epiphytes In The Kihansi Gorge Forest, Tanzania, Hashim Mangosongo, Martin Griffiths Aug 2019

Species Composition, Diversity And Distribution Of Vascular Epiphytes In The Kihansi Gorge Forest, Tanzania, Hashim Mangosongo, Martin Griffiths

Tanzania Journal of Science

This study aimed at assessing the species composition, diversity and distribution of vascular epiphytes in the Kihansi Gorge Forest. Epiphytes sampling was conducted in twenty sampling plots established in the strip of riverine forest within the Kihansi Gorge. All vascular epiphytes growing on trees (phorophytes) rooted in the plots were sampled using ground-based technique as the method of access to the tree canopies.  A total of 476 epiphyte individuals belonging to 20 species and 10 families were recorded in the study area. Among the twenty species recorded, Drynaria laurentii was the most abundant and most widely distributed in the study …


Morphological And Molecular Identification Of Pratylenchus Goodeyi Associated With Banana In Tanzania, Doreen Mgonja, Gladness Temu, Magreth Mziray, Beatrice Kashando, Ambilikile Mwenisongole, Minza Masunga, Sylvester Lyantagaye4, Nessie Luambano Aug 2019

Morphological And Molecular Identification Of Pratylenchus Goodeyi Associated With Banana In Tanzania, Doreen Mgonja, Gladness Temu, Magreth Mziray, Beatrice Kashando, Ambilikile Mwenisongole, Minza Masunga, Sylvester Lyantagaye4, Nessie Luambano

Tanzania Journal of Science

Banana (Musa sp. [L]) is an important staple food and cash crop for about 30 percent of the total population in Tanzania. Root lesion nematodes, particularly Pratylenchus goodeyi, are among the major pests affecting banana production in Tanzania. Morphological, morphometric and molecular approaches are required for accurate identification of nematodes. In this study, 80 P. goodeyi isolates from major banana growing areas in Tanzania were identified. Sequence analysis of part of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) regions of ribosomal DNA (rDNA) confirmed the identity of P. goodeyi. DNA sequences were analysed using Geneious …


Agro-Morphological Characterization Of The Wild Rice (Oryza Longistaminata) Populations From Selected Areas Of Tanzania, Hashim M. Mangosongo, Herbert V. Lyaruu, Emmarold E. Mneney Mar 2019

Agro-Morphological Characterization Of The Wild Rice (Oryza Longistaminata) Populations From Selected Areas Of Tanzania, Hashim M. Mangosongo, Herbert V. Lyaruu, Emmarold E. Mneney

Tanzania Journal of Science

The study assessed the variations in ten agro-morphological characters among and within the wild rice (O. longistaminata) populations from four selected areas, namely Bagamoyo, Kibaha, Kilombero and Mbarali in Tanzania. The study was carried out through measurement of ten quantitative agro-morphological characters, based on morphological descriptors for wild and cultivated rice species developed by Bioversity International and International Rice Research Institute (IRRI). Measurement of morphological characters of O. longistaminata plants was done soon after heading. One-way Analysis of Variance (ANOVA) was used to compare the variations in quantitative characters between O. longistaminata populations from the four study areas. The results …


Plant Diet Selectivity And Some Environmental Parameters At Foraging Sites Of Wattled Crane (Bugeranuscarunculatus) In Malagarasi Wetlands, Tanzania, Jasson R.M. John Mar 2019

Plant Diet Selectivity And Some Environmental Parameters At Foraging Sites Of Wattled Crane (Bugeranuscarunculatus) In Malagarasi Wetlands, Tanzania, Jasson R.M. John

Tanzania Journal of Science

Wattled Crane Bugeranuscarunculatusis a threatened wetland-dependent bird species of sub-Saharan Africa with a declining population throughout its range due to habitat degradation. Ecological studies on this species have come from large populations in Botswana and Zambia. Little is known of the isolated small populations, especially those in Tanzania where about 200individuals survive. This study reports observations made on vegetation height, water depth and consumption of plant tubers at foraging locations of the Wattled Crane in Malagarasi-MuyovoziRamsar Site. Foraging birds preferred wet habitats with short vegetation. Birds fed mainly on Pycreusnitidus and Cyperusarticulatusplant tubersbut not those of Eleocharis, a chief food …


Cytotoxic And Antimicrobial Activities Of The Constituents Of Ten Plant Species From Tanzania, Joan J. E. Munissi Mar 2019

Cytotoxic And Antimicrobial Activities Of The Constituents Of Ten Plant Species From Tanzania, Joan J. E. Munissi

Tanzania Journal of Science

This paper reports on the antimicrobial and cytotoxicity activities of crude extracts from the plant species Clemaptosis scabiosifolia (Ranunculaceae), Diospyros mafiensis (Ebenaceae), Dolichos kilimandscharicus (Leguminosae), Gnidia kraussiana (Thymelaeaceae), Hugonia castaneifolia (Linaceae), Neorautanenia mitis (Pappilionaceae), Tagetes minuta (Asteraceae), Uvaria acuminata (Annonaceae), Vernonia amygdalina (Asteraceae) and Zanha africana (Sapindaceae). All crude extracts were screened for their cytotoxicities in the brine shrimp lethality test (BST). Fractions and pure compounds from Hugonia castaneifolia, Diospyros mafiensis and Uvaria acuminata were tested for their cytotoxicity and antimicrobial activities against ten microorganisms, namely Candida albicans, Bacillus anthracis, Escherichia coli, Klebsiela pneumoniae, Proteus sp., Pseudomonas aeruginosa, Salmonella typhimurium, …


Prevalence Of Zoonotic Parasites In Stray Dogs In Rural Communities, Tanzania, Winifrida Kidima Mar 2019

Prevalence Of Zoonotic Parasites In Stray Dogs In Rural Communities, Tanzania, Winifrida Kidima

Tanzania Journal of Science

The aim of this study was to identify and establish the baseline information on the prevalence of zoonotic parasites in stray dogs in Tanzania. A cross sectional study was conducted in Katavi, Arusha and Rukwa Regions in Tanzania. Intestinal parasites were analyzed from faecal samples collected from 364 dogs using modified sedimentation technique. Sixty-sixpercentage (66%) of the faecal samples were positive for gastrointestinal parasites while 7% had mixed infections, 27% were negative for the parasites. Higher proportions of the faecal samples were positive for gastrointestinal parasites in Arusha and Rukwa compared to Katavi (p< 0.0001). The prevalence of Ancylostomacaninum(49%) was higher compared to that of other parasites (p <0.0001). Prevalence of A. lumbricoideswas significantly higher compared to that of E. granulosusand S. stercoralis(p = 0.0046). There was a positive association between parasite positivity rates and collecting dog’s faecal samples near domestic animals (p < 0.00001). Parasite diversity was higher in stray dogs from Katavi and Arusha (p > 0.05). There was no association between …


In Silico Pharmacokinetic And Molecular Docking Studies Of N-Cinnamoyltetraketide Derivatives As Inhibitors Of Cyclooxygenase-2 Enzyme, Stephen S. Nyandoro, Daniel M. Shadrack, Joan J. E. Munissi, Egid B. Mubofu Jul 2018

In Silico Pharmacokinetic And Molecular Docking Studies Of N-Cinnamoyltetraketide Derivatives As Inhibitors Of Cyclooxygenase-2 Enzyme, Stephen S. Nyandoro, Daniel M. Shadrack, Joan J. E. Munissi, Egid B. Mubofu

Tanzania Journal of Science

Recent phytochemical analysis of Toussaintia orientalis leaves yielded series of novel bioactive N-cinnamoyltetraketide derivatives namely toussaintines A-G (t_1 - t_8) some portraying cytotoxicity against the triple negative aggressive human breast cancer cell line (MDA-MB-231) among other potencies. Despite having broad bioactivity spectrum, their general drug-likeness profiles and mode of action (simulated or actual) targeting any enzyme remains uninvestigated. In silico pharmacokinetic, drug-likeness descriptors and molecular docking of the compounds t_1-t_8 targeting inhibition of cyclooxygenase-2 (COX-2) enzyme were evaluated. The Lipinski Rule of Five heralded the pharmacokinetic properties of the studied metabolites. The studied compounds were docked with COX-2 following already …


One-Compound-Two-Pharmacophores: Design, Synthesis And Antimalarial Activities Of Chloroquinoline-Cassiarin, Thobias M. Kalenga, Monica M. Ndoile, Quintino A. Mgani Jul 2018

One-Compound-Two-Pharmacophores: Design, Synthesis And Antimalarial Activities Of Chloroquinoline-Cassiarin, Thobias M. Kalenga, Monica M. Ndoile, Quintino A. Mgani

Tanzania Journal of Science

This paper describes the design and synthesis of a potential antimalarial compound, chloroquinoline-cassiarin (6) using 4,7-dichloroquinoline and the natural product barakol (1) extracted from the leaves of Cassia siamea as starting materials. In this paper a one compound-two-pharmacophores concept is introduced in which coupling of two known pharmacophores into one has been described. The concept is not only useful to organic synthesis but also to drug development researches. The synthesized compound 6 exhibited potent in vitro antimalarial activity at IC50 = 0.51 µM, and low toxicity where at 20 µM, 80% viability of HeLa cells was observed.


Bioactivities Of Extracts, Debromolaurinterol And Fucosterol From Macroalgae Species, Sartaz Begum, Stephen S. Nyandoro, Amelia S. Buriyo, John J. Makangara, Joan J. E. Munissi, Sandra Duffy, Vicky M. Avery, Mate Erdelyi Jul 2018

Bioactivities Of Extracts, Debromolaurinterol And Fucosterol From Macroalgae Species, Sartaz Begum, Stephen S. Nyandoro, Amelia S. Buriyo, John J. Makangara, Joan J. E. Munissi, Sandra Duffy, Vicky M. Avery, Mate Erdelyi

Tanzania Journal of Science

Parasitic diseases including malaria, and other numerous microbial infections and physiological diseases are threatening the global population. Tanzanian coast shores are endowed with a variety of macroalgae (seaweeds), hitherto unsystematically explored to establish their biomedical potentials. Thus, antiplasmodial activity using malarial imaging assay, antimicrobial activity using microplate dilution technique, antioxidant activity using DPPH radical scavenging method and cytotoxicity using brine shrimp test were carried out on crude extracts from the selected species of algae (Acanthophora spicifera, Cystoseira myrica, Cystoseira trinodis, Laurencia filiformis, Padina boryana, Sargassum oligocystum, Turbinaria crateriformis, Ulva fasciata and Ulva reticulata) occurring along the coast of Tanzania. The …


Determination Of Toxic Diethylene Glycol In Toothpastes In Dar Es Salaam By High Performance Thin Layer Chromatography And Colorimetric Methods, John Andrew Marco Mahugija Jul 2018

Determination Of Toxic Diethylene Glycol In Toothpastes In Dar Es Salaam By High Performance Thin Layer Chromatography And Colorimetric Methods, John Andrew Marco Mahugija

Tanzania Journal of Science

The aim of this study was to determine the presence and the levels of diethylene glycol (DEG) in toothpaste products commonly used in Dar es Salaam. Forty five samples of different brands of imported and locally manufactured toothpastes were collected and processed prior to analysis. High performance thin layer chromatography (HPTLC) method was used for the qualitative detection of diethylene glycol in the toothpastes. In quantitative analysis, a colorimetric method that involved ultraviolet–visible (UV-Vis) spectrophotometric measurements of absorbances of prepared coloured samples and standards was used. DEG was found in 37.8% of the tested samples. The concentrations of the DEG …