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Evaluation Of Chemical Treatments And Intrinsic Factors That Affect The Mutagenic Potential Of Aflatoxin B1-Contaminated Corn., Armando Burgos-Hernandez

LSU Historical Dissertations and Theses

In the present project the use of ammoniation and fermentation as an integrated process was studied in order to give aflatoxin-contaminated corn an alternative use. Aflatoxin-contaminated corn can be fermented to obtain fuel alcohol and decontaminated distiller's dried solid grains (DDSG) can be used for animal feed. The efficacy of a fermentation process, with the inclusion of ammonium persulfate, for the decontamination of aflatoxin B$\sb1$-contaminated yellow corn was studied, as well as the effect of peroxides on the ethanol production. Addition of 2.0% ammonium persulfate decreased the aflatoxin B$\sb1$ levels by 87%. Peroxides did not have an effect on ethanol …

Aflatoxin B(1) And Fumonisin B(1) Co-Contamination: Interactive Effects, Possible Mechanisms Of Toxicity, And Decontamination Procedures., Rebeca Lopez-Garcia

LSU Historical Dissertations and Theses

The current study evaluated interactions between the mycotoxins aflatoxin B$\sb1$ (AFB$\sb1$) and fumonisin B$\sb1$ (FB$\sb1$) in pure systems as well as in corn. During storage at 25$\sp\circ$C with high moisture (ca. 50%), corn contaminated with Aspergillus flavus (L13) and/or Fusarium moniliforme (A00102) presented different aflatoxin and fumonisin levels. Toxin production/degradation varied over storage time (0-45 days). Evaluation of the mutagenicity of unifungal or bifungal corn extracts with the Salmonella/microsomal assay (tester strains TA-100 and TA-98) with metabolic activation (S9) showed positive mutagenic responses. Different ratios of pure AFB$\sb1$/FB$\sb1$ produced divergent mutagenic responses (tester strains TA-100 and TA-98) with S9. The …

Characterization And Utilization Of Flavors From Undersized Crawfish., Feng Chen

LSU Historical Dissertations and Theses

Undersized processed crawfish are proposed to be a resource for effective recovery of crawfish flavors. Tail puree from undersized crawfish was hydrolyzed using protease APL-440 through response surface methodology to enrich flavor production. Optimal hydrolytic conditions were determined to be pH $8{\sim}9,\ 65\sp\circ$C, 0.3% (V/W) enzyme/substrate ratio with the reaction maintained at 33.3% puree concentration over 60 min period. Effects of storage time and extraction method on the crawfish flavor profile were evaluated. Shorter storage time gave superior flavor stocks with less oxidized flavors. The shelf-life of frozen crawfish puree was at least six months. Atmospheric simultaneous distillation-solvent extraction (A-SDE) …

Rapid Assessment Of The Bacteriological Quality Of Milk By The Use Of Adenosine Triphosphate Bioluminescence., Pushpa John Samkutty

LSU Historical Dissertations and Theses

Raw milk samples (n = 246) and pasteurized milk samples (n = 104) were analyzed to determine adenosine triphosphate (ATP) and compared with the standard plate count (SPC). An ATP filtration method was used to filter milk samples prior to ATP determination, which was measured in relative light units (RLU). The ATP assay took approximately 7 minutes to complete and could allow rapid prediction of SPC which is a measure of raw milk quality. Linear regression analysis was performed on data from three raw milk treatments defined as; fresh milk samples, samples preliminarily incubated at 12.8 and 15.6$\sp\circ$C for 18 …

Mating System Differences And Genetic Effects For Preweaning Traits In Beef Cattle In The Gulf Coast Region Of The United States., Dian Allison Williams

LSU Historical Dissertations and Theses

Preweaning data collected on 1,180 calves in generation five of a crossbreeding project were analysed. The systems involved were first-cross (F$\sb1$, three-breed terminal, two-, three-, and four-breed rotational and rotational-terminal. Angus (A), Brahman (B), Charolais (C), and Hereford (H) breeds were used in the F$\sb1$ and rotational phases, while Gelbvieh (G) and Simmental (S) were the terminal sire breeds. The traits studied were birth weight (BWT), preweaning gain (ADG), 205 d weight (WWT), and weaning height (WHT). Least squares analysis of variance procedures were used for comparisons of mating systems for all traits. Estimates of direct and maternal additive (Ig, …

The Dissident : Vol. 3, No. 3 - September 1997, The Dissident (Portland Me)

The Dissident

The Dissident was a monthly journal of ideas and analysis (published June 1995 – January 2000 in Portland, Maine) to provide a forum for Maine writers, activists, and scholars committed to social justice and political change.

Preventing Calf Losses, Clell Bagley

All Current Publications

Many beef producers and practicing veterinarians noted there were higher than normal calf death losses during the 1993 calving season. We were especially aware of the increased losses here in the western states, but there were also heavy losses in the midwest and east, so the problem was very widespread.

The Dissident : Vol. 2, No. 10 - April 1997, The Dissident (Portland Me)

The Dissident

The Dissident was a monthly journal of ideas and analysis (published June 1995 – January 2000 in Portland, Maine) to provide a forum for Maine writers, activists, and scholars committed to social justice and political change.

G97-1320 Feeding To Maximize Milk Solids, Rick J. Grant

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide describes feeding guidelines to increase production of solids-corrected milk.

Proper feeding management of the dairy herd can improve the economy of production and provide for a healthier cow. Feeding to increase production of milk with maximum levels of milk fat and protein is essential for achieving these benefits.

Milk solid components include fat, protein, lactose, and minerals. Normal values for milk fat range from 3.7 percent (Holstein) to 4.9 percent (Jersey); milk protein ranges from 3.1 percent (Holstein) to 3.8 percent (Jersey). Lactose is usually 4.6 to 4.8 percent for all breeds and minerals (ash) average .74 percent. …

Beta-Carotene In The Bovine Corpus Luteum, Stephen Grimmell Judd

Doctoral Dissertations

There have been conflicting reports which associate a low dietary intake or plasma concentration of beta-carotene (BC) with decreased fertility in dairy cows. The bovine corpus luteum (CL), a transient endocrine gland formed subsequent to ovulation, produces the steroid hormone progesterone which sustains pregnancy and is associated with fertility. Because of its high concentrations of the antioxidant nutrient beta-carotene, the CL was examined to identify an in vivo function for BC, other than that as a precursor of retinol. Five non-lactating Jersey cows were fed a basal ration containing a low BC concentration. Each cow received either 0, 1.6, 8.0, …

Ruminally Undegraded Protein And Ruminally Protected Amino Acids For Dairy Heifers, Brian David Garthwaite

Doctoral Dissertations

A series of growth experiments was conducted to evaluate the feeding of ruminally undegraded protein alone, or in combination with ruminally protected lysine and methionine. Eighty Holstein heifer calves were blocked by date of birth as they became available at 6 wk of age and assigned to a 2 x 2 factorial arrangement of treatments. Main effects of the 2 x 2 factorial were amount of ruminally undegraded protein in diet dry matter and whether ruminally protected lysine arid methionine were supplemented to diets. Four phases of growth were evaluated: Phase (1) 6 wk to 100 kg; Phase (2) 100 …

G97-1342 Feeding Value Of Alfalfa Hay And Alfalfa Silage, Terry L. Mader, Todd Milton, Ivan G. Rush, Bruce Anderson

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide discusses the feeding value of alfalfa under different harvesting, storage and feeding methods, as compared to other protein sources.

In Nebraska, alfalfa is used primarily as a protein source. However, for cow-calf producers it can sometimes be an economical energy source, depending on the prevailing price of feed grains and other sources of energy.

Alfalfa is generally harvested as dry hay or as silage (65 percent moisture). Ensiling at 45 to 55 percent moisture is also common, particularly if the alfalfa is stored in an upright oxygen-limiting structure. Regardless of harvesting and storage methods, some dry matter and …

Ec97-274 Management Of Replacement Gilts For Efficient Reproduction, Donald G. Levis

University of Nebraska-Lincoln Extension: Historical Materials

Successfully introducing replacement gilts into the breeding herd is an important aspect of breeding herd efficiency. To attain herd efficiency, females must ovulate adequate numbers of viable ova, express estrus, show willingness to mate and conceive in a regular manner. If these qualities are properly developed, pregnancy rate and litter size will be maximized. A gilt development and management program needs to be designed for gilts from birth to farrowing their first litter.

G97-1313 Designing Preventive Health Management Programs For Cattle Producers, Dicky D. Griffin, Louis Perino, Gary Rupp, Eddie Hamilton

University of Nebraska-Lincoln Extension: Historical Materials

The NebGuide details recommendations for a beef cattle herd immunization program.

This NebGuide presents two flow sheets which detail the recommendations for a beef cattle herd immunization program: the Calf through Weaning Preventive Health Management Flow Sheet and the Heifers, Cows and Bulls Preventive Health Management Flow Sheet. While this NebGuide emphasizes the procedures conducted on cattle at each cattle handling opportunity, it is very important to note the role a high quality nutritional program plays in building a total herd health program. You are encouraged to work with a qualified beef cattle nutritionist and your veterinarian when developing your …

Evaluation Of Water-Seeded No-Till Rice Culture With Respect To Weeds And Seedling Disease., Roland Detleo Mendt

LSU Historical Dissertations and Theses

Field studies were conducted over three years to evaluate preplant postemergence herbicides for red rice control in rice under no-till water-seeded culture. Application of paraquat or sulfosate plus thiobencarb and paraquat alone 3 days before seeding (DBS) resulted in the greatest red rice control 11 weeks after seeding (WAS). Rice yield was increased and percent red rice in rice grain decreased when paraquat or sulfosate was applied in combination with thiobencarb compared with the nontreated control. Rice yield was increased when herbicides were applied 3 and 7 DBS as compared with 14 and 28 DBS. Aqueous extracts of cursed buttercup, …

A Case-Ready Modified-Atmosphere Packaging System For Beef., Brian Scott Smith

LSU Historical Dissertations and Theses

Beef tenderness and color stability were evaluated in conventional and modified atmosphere packaging (MAP) systems. Equal portions of inside rounds removed immediately (PM0) or at 48 h postmortem (PM48) were vacuum packaged (VP) whole or cut into steaks and packaged in 80%N$\sb2$:20%CO$\sb2$ MAP. On day 12, MAP environments were exchanged for 80%O$\sb2$:20%CO$\sb2$. Calpastatin activity was lower at 2 d postmortem for PM48 steaks than PM0 steaks. Electrophoresis indicated more degradation of 50 and $>$250 kDA compounds at 2 d postmortem for PM48 than PM0 steaks. Steaks from PM48 required less shear force than PM0 steaks. HunterLab 'L', 'a' and …

Amino Acid Requirements And Crystalline Amino Acids For Finishing Pigs., Theresia Alice Knowles

LSU Historical Dissertations and Theses

This research was conducted to obtain a better understanding of the amino acid needs of finishing pigs. Twelve experiments were conducted using the urea nitrogen method to determine the lysine requirement of gilts and barrows. Two experiments were conducted to determine the optimum ratio of total sulfur amino acids to lysine for finishing pigs (74 to 111 kg); and three experiments were conducted to evaluate the net energy content of low crude protein, crystalline amino acid supplemented diets for finishing pigs (70 to 117 kg). The lysine requirement of gilts, as determined by the urea nitrogen method, decreased as body …

The Production Of Glucooligosaccharides By Leuconostoc Mesenteroides Atcc 13146 And Lipomyces Starkeyi Atcc 74054., Sun Kyun Yoo

LSU Historical Dissertations and Theses

Glucooligosaccharides of less than DP 10 were produced either by mixed cultures of Leuconostoc mesenteroides ATCC 13146 and L. starkeyi ATCC 74054, or by an acceptor reaction produced by Leuconostoc mesenteroides ATCC 13146. The yields of glucooligosaccharides produced by mixed culture and acceptor reaction reached up to 49 and 90% of theoretical yield. The composition of carbohydrates at the end of fermentation by mixed culture was 31.4% oligosaccharides, 17.6% dextran, 31.4% mannitol, 2.1% fructose, and 2.4% of sucrose. The composition of carbohydrates at the end of fermentation by acceptor reaction was 32.7% pannose, 30.4% branched isomaltotetraose (DP 4), 21.7% branched …

Evaluation Of Sampling Gear For Predicting Harvest Size, Yield And Incidence Of Stunting In Crawfish Ponds., Jimmy Lee Avery

LSU Historical Dissertations and Theses

Dip-nets, test traps and experimental drop samplers were evaluated for their potential to predict crawfish (Procambarus spp.) yields and size distribution at harvest. Field studies were conducted at the Rice Research Station, LSU Agricultural Center, Crowley, Louisiana, between 1991 and 1996 in 82 (0.16-0.2 ha) earthen impoundments. Fields were managed to simulate rice-crawfish systems typical of the southwestern and south-central Louisiana. Multiple linear regression analysis was used to determine the relationship among catch-per-unit-effort (CPUE) of sampling gear (alone and in combination) with respect to yield in weight (kg/ha), yield in number (crawfish/ha) and size distribution at harvest. Relative abundance of …

Reduction And Management Of Risks Associated With Aflatoxin And Fumonisin Contamination In Corn., Socrates Trujillo

LSU Historical Dissertations and Theses

Aflatoxins and fumonisins, secondary mold metabolites, occur naturally as co-contaminants in corn. Health and economic risks associated with this contamination of food and feed have been the focus of much research worldwide. Research programs resulting from the discovery of aflatoxins in the 1960's has been used to guide study programs for fumonisins. The establishment of food safety programs, including mycotoxin decontamination procedures, must be evaluated for further application. The evaluation of selected procedures which reduce the risks associated with the contamination of these mycotoxins in food utilized for human and animal consumption was the focus of this study. This study …

Production Of Transgenic Channel Catfish., Mark Christian Bates

LSU Historical Dissertations and Theses

The channel catfish industry has experienced tremendous growth over the last several years. This growth has triggered more intensive culture practices resulting in disease outbreaks and devastating mortalities. Medicated feeds and vaccination have been of limited use. This research presents studies on techniques to produce disease resistant channel catfish by gene transfer. The gene used was the cecropin B gene from the giant silkworm moth Hyalophora cecropia, controlled by an acute phase response (APR) promoter also from H. cecropia. The objectives of this study were to: (1) determine if an early maturing population of catfish from Lake Maurepas, Louisiana, could …

Lmo's: Treasure Chest Or Pandora's Box, Michael S. Baram

Faculty Scholarship

Biotechnology is beginning to trans- A form agriculture across the globe. After thousands of years of traditional plant and animal breeding, and centuries of mechanization and chemical application, genetic research has opened a Pandora's box of living modified organisms (LMOs) designed to improve the productivity and efficiency of commercial agriculture. A multitude of transgenic crops and animals is now being introduced into commerce by biotechnology companies, and b nations are puzzling out how to appropriate the benefits and manage the risks.

American biotechnology companies and agencies are the leading proponents of using LMOs. They claim that two decades of costly …

Optimizing Lysine And Methionine Nutrition During The Periparturient And Postparturient Periods, Vicky Marie Wasserstrom

Doctoral Dissertations

The primary objectives of experiment 1 were to determine the effects of changes in feed intake associated with the periparturient period (late gestation to early lactation) on passage of nitrogen fractions and amino acids to the small intestine and to determine if changes occurred in the profile of amino acids presented to the small intestine that could not be explained by diet composition. A secondary objective was to obtain preliminary data on changes in plasma concentrations of nonesterified fatty acids during the periparturient period when cows were fed nutrient-dense diet prepartum diets. Beginning 14 days before expected calving, 10 multiparous …

Effects Of Organic Waste Amendments, Herbicides, And Weed Hosts On Pythium Root Rot Of Sugarcane., Nimal Mudiyans Dissanayake

LSU Historical Dissertations and Theses

Three objectives that could lead to improved understanding and management of Pythium root rot of sugarcane were addressed. First, the potential of amending soil with different organic wastes to suppress root rot was evaluated, and the biological and chemical properties of wastes that may contribute to disease suppression were identified. Secondly, herbicide interactions with root rot affecting sugarcane growth were assessed. Finally, the role of weeds in disease epidemiology was investigated. Greenhouse and laboratory studies indicated soil amendment with some organic wastes offers a sustainable disease management option through biological control. Amendment with different wastes had variable effects. Sewage sludge, …

Biological Control Of Pythiaceous Fungi Causing Death Of Longleaf Pine Seedlings During Cold Storage., Xiaoan Sun

LSU Historical Dissertations and Theses

Pythium dimorphum Hendrix & Campbell and other Pythium species were determined to be pathogenic fungi that cause the death of longleaf pine seedlings during cold storage. Pathogenicity of these Pythium isolates as determined by infecting newly-germinated slash pine seedlings in vitro was confirmed in field evaluations. An in vitro dual culture screening system was effectively used for selecting Trichoderma isolates capable of killing P. dimorphum. Selected Trichoderma isolates and Gliocladium virens produced volatile and/or non-volatile antibiotics on solid media and in liquid cultures, but antibiotic production could be affected by medium type, pH, and culture age, depending on species or …

An Attempt To Classify The Production And Regulation Of Cyclooxygenase-2 During Ovulation And Luteolysis., James Robert Broussard

LSU Historical Dissertations and Theses

A series of experiments were designed to determine the cyclooxygenase (COX) isoform responsible for prostaglandin release during ovulation and luteolysis. Initially, a reliable, serum-free culture system was developed to produce large numbers of bovine granulosa cells originally harvested from the small antral follicles of abattoir ovaries. This method allowed adequate numbers of cells to be obtained for the subsequent study involving cyclooxygenase regulation. In a second experiment, bovine granulosa cells from small antral follicles were isolated and cultured as in the first experiment for 6 days. Granulosa cells were then exposed to medium alone or medium containing arachidonic acid (10 …

Thiamine, Riboflavin, And Sensory Stability Of Irradiated Brown Rice., Linda Chamberlain Douglas

LSU Historical Dissertations and Theses

While brown rice is nutritionally superior to white rice, it is not shelf stable, becoming rancid in a matter of weeks. Preservation by refrigeration or freezing is expensive and not universally available. Therefore, other preservation methods, such as aqueous ethanol extraction of lipids, have been studied to overcome this problem, but these methods also have limitations. The effects of gamma-irradiation on the physicochemical properties and lipase activity in brown rice has been well documented. Gamma-irradiation has been shown to reduce lipase activity, presumably preserving sensory quality. A sensory study of 2 Louisiana grown varieties (Mars and Lemont) of irradiated brown …

The Effects Of Timber Management Activities On Understory Plant Succession In Young Loblolly Pine (Pinus Taeda) Plantations., Donald Paul Reed

LSU Historical Dissertations and Theses

An understory vegetative succession study was conducted on the LSU Lee Memorial Forest near Bogalusa, Louisiana. Research blocks were established within fifteen-year-old overstocked loblolly pine (Pinus taeda) stands, in specific areas classified as with or without a history of prescribed burning for the current timber rotation. Silvicultural treatments involved precommercially thinning randomly selected blocks to various stand densities, along with the establishment of unthinned control areas. Growth rates of residual trees were determined from tree diameter measurements, on all timbered plots. Burn-history areas received a further treatment, involving the mechanical destruction of all timber on specified areas, followed by site-preparation …

Interrelationships Between Meloidogyne Incognita And Rotylenchulus Reniformis On Soybean., Salliana Ryan Stetina

LSU Historical Dissertations and Theses

The replacement series approach was used to evaluate competition between Meloidogyne incognita (Mi) and Rotylenchulus reniformis (Rr). In greenhouse tests, soil in pots containing 'Davis' soybean was not infested or infested with 1,000 vermiform nematodes in the following Mi:Rr ratios: 100:0, 75:25, 5:50, 25:75, and 0:100. After 86 days, relative nematode yields (RNYs) (number of each species in mixed culture divided by number in nonmixed culture) were calculated based on soil populations. Calculated values were plotted and the resulting line compared with a reference line representing equal inter- and intraspecific competition. RNYs for Mi were higher than predicted where Mi …

Image Analysis Quantification Of Decondensation Of Bovine Spermatozoa And Pcr Amplification Of A Y Chromosome Specific Sequence., Hilde Christina Steinholt

LSU Historical Dissertations and Theses

The first objective of this research was to develop a decondensation treatment for bovine spermatozoal DNA that worked consistently across bulls and breeds. The second objective was to use the decondensed DNA for polymerase chain reaction analysis of levels of Y-bearing spermatozoa in different ejaculates and in sex sorted spermatozoa. Decondensation of spermatozoa with dithiothreitol and potassium hydroxide was compatible with in vitro amplification and did not inhibit amplification. The polymerase chain reaction and image analysis were used to differentiate between proportions of Y spermatozoa by measuring fluorescent intensities of electrophoresed, polymerase chain reaction-amplified DNA. There were differences in intensities …