Digital Commons Network^™

Macroplastic Litter In A Rural Lake: Biomass Characteristics Relative To Natural Surfaces, Alyssa Younus

Undergraduate Research and Engagement Symposium

Plastic pollution is increasing worldwide and persists in aquatic ecosystems due to its durability. In lakes, plastic can act as habitat for microbes, invertebrates, and fish. This study is part of a larger experiment examining how floating debris influences aquatic communities. Conducted in summer 2024 at Lake 302 in Ontario’s Experimental Lakes Area, floating plots contained either plastic litter or wood. After 56 days, biofilm samples were analyzed for chlorophyll a concentrations and biofilm biomass. Both were significantly higher on wood, suggesting plastic’s properties limit algal growth. These findings indicate plastic may be a less suitable habitat and could impact …

Protein-Lipid Interactions Using Model Membrane And Native Membrane Vesicles, Nick Pufall

Undergraduate Research and Engagement Symposium

This project explores how proteins interact with lipid membranes using both simplified model vesicles and more complex native membranes. Small unilamellar vesicles (SUVs) were synthesized and characterized to study lipid binding with bovine serum albumin using fluorescence techniques. While model membranes provide control, they lack the complexity of real cellular environments, so native membranes were also investigated to better capture protein–lipid interactions. By comparing these systems, this work aims to better understand how lipids are transported and delivered within cells, and how these processes influence signaling and membrane protein function.

Β-Glucosidase B Mutant P41l Characterization And Analysis, Amal Naqvi, Caroline Machola

Undergraduate Research and Engagement Symposium

This study characterized the β-glucosidase B (BglB) mutant P41L within the Design2Data (D2D) framework. We hypothesized that substituting proline with leucine at position 41, a non-active-site residue, would maintain catalytic efficiency (kcat/Km) but decrease thermal stability (T50) relative to wild-type (WT) BglB. Foldit modeling, plasmid sequencing, expression in E. Coli, and affinity purification were used to validate the construct. Kinetic parameters with p-nitrophenyl-β-D-glucopyranoside and T50 values across 30–50 °C were obtained via plate-reader assays and D2D fits. P41L displayed higher catalytic efficiency and a T50 comparable to WT, refuting our hypothesis and suggesting an efficiency-enhancing, stability-neutral mutation.

The Impact Of Adverse Childhood Experiences (Aces) On The Development Of Reckless Behavior In Adulthood, Cheyenne Harvey

GS4 Student Scholars Symposium

Current literature suggests that there is a correlation between Adverse Childhood Experiences (ACEs) and behaviors in adulthood (e.g., substance use, crime). Research also shows that childhood trauma can lead to increased risk-taking or reckless behavior later in life. A study by Assini-Meytin et al. (2021) found that having a higher number of ACEs is linked to more risky behaviors in adulthood. These risky behaviors are often ways to deal with emotional problems caused by early stress. Anda et al. (2006) points out that these experiences can cause lasting changes in brain development, especially in areas that handle impulse control. The …

Manual Tissue Slicer Designed For Ovarian Tissue Cryopreservation, Levon Shukrian, Caleb Anene, Daniel Archer, Derek Works, Jack Durham, Joby Anthony, Jumana Rizk, Maria Mendoza Lopez, Shanice Rerrie

Liberty University Research Week

Fertility preservation techniques such as ovarian tissue cryopreservation and autotransplantation provide important options for patients at risk of primary ovarian insufficiency due to medical treatments or disease. A critical step in this process is the preparation of uniform ovarian cortical slices (0.5–1.5 mm thick) that preserve follicular architecture prior to cryopreservation. However, the discontinuation of the Stadie-Riggs tissue slicer, previously used for fresh tissue slicing, has created a significant gap in both translational research and clinical practice. To address this need, a compact manual ovarian tissue slicer was designed and constructed using biocompatible 316L stainless steel and borosilicate glass. The …

Morphological Identification Of Echinostomas Found Within Dabbler And Diving Ducks, Sammy Schneider, Emma Harms, Miana Risser, Madison Becker

Research & Creative Achievement Day

Most ducks can be separated into two categories, dabblers or divers, depending on their feeding guild. Dabbler ducks are surface feeders and consume vegetation while diver ducks feed below the surface and consume aquatic animals. The Echinostoma species present in ducks may vary depending on their feeding guild. Three species of ducks from various regions of Wisconsin were donated by hunters for examination of endoparasites, particularly those from the genus Echinostoma. The sites of collection included Trempealeau, Marionette, and Onalaska. One species of dabbler duck, Gadwall (Mareca strepera), and two species of diver ducks, Lesser Scaup (Aythya affinis) and Canvasback …

Comparing Dissection Vs Literature Data Of Cestodes In Ducks, Sophia B. Cross

Research & Creative Achievement Day

Cestodes are a class of parasites that are often found in vertebrate animals, specifically ducks. Research conducted by Winona State University students showed that cestodes were the most prevalent type of parasites found within ducks. However, outside research provides controversy as to whether this is actually true. Ducks were dissected by Winona State students over the past seven years, and their internal organs were carefully inspected under a light microscope. Ducks were donated by hunters, mostly harvested along the Mississippi River, from Buffalo City, WI to Winona, MN. Parasites were obtained, stored, and labelled from each duck. A dichotomous key …

Family Is Forever: A Qualitative Study Of The Impacts Of Adoption On Adoptees, Rebekah Hedlund

Liberty University Research Week

This study explores the lived experiences of adoptees and the ways in which adoption has impacted them. Grounded in communication theory of identity (CTI), this research examines how adoptees express their identities throughout their lives via communication. Using a qualitative approach, in-depth semi-structured interviews were conducted with adoptees over the age of 18 from diverse backgrounds. The findings revealed three key themes from adoptees: identity, education, and opportunity. Participants described identity to be an ongoing and evolving process throughout life, often influenced by biological origins and family dynamics. The theme of education emerged through participants’ desire for educating potential adoptive …

Per- And Polyfluoroalkyl Substances (Pfas)Exposure And Its Influence On Fetal And Early Childhood Development, Tahirou Diallo

Liberty University Research Week

Per- and polyfluoroalkyl substances (PFAS) are persistent synthetic chemicals ubiquitous in environmental and biological matrices, with growing evidence implicating prenatal exposure in adverse developmental outcomes. This systematic review synthesizes cohort-based evidence published between 2016 and 2026 to evaluate the effects of maternal PFAS exposure on fetal growth and early childhood development. Studies were identified through PubMed, Google Scholar, and Scopus, and were included if they assessed prenatal PFAS concentrations in relation to birth weight, gestational age, fetal growth trajectories, or childhood anthropometric outcomes. Findings across multiple cohorts consistently link higher prenatal exposure to perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) …

Sexual Differentiation In A Unique Vertebrate Lineage: Mapping The Development Of Genitalia In The Lizard Anolis Sagrei, Alina Mathew

Undergraduate Research and Engagement Symposium

• Genitalia are some of the most rapidly evolving structures on the vertebrate body, yielding an immense amount of genital diversity. Lizards and snakes have paired genitalia, while all other amniotes have a single, midline phallus. The lizard, Anolis sagrei is an important organism to study the development of genitalia to understand differences between paired and single genitalia. Genital development in A. sagrei begins with two genital swellings in both males and females. Then as the anole progresses, the swellings either elongate into hemipenes or reduce to hemiclitores. The objective of my research was to determine the normal course of male …

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Microbial Responses To Pharmaceutical Pollution In Streams, Darian Bolden

Undergraduate Research and Engagement Symposium

Pharmaceuticals, which include prescription and non-prescription drugs, have been detected in human-impacted streams throughout the world, raising concerns about the potential ecological effects of these drugs. The goal of my project was to assess the potential effects of pharmaceutical pollution on leaf litter decomposition by microorganisms as an indicator of stream health. We added a mixture of eight antibiotics to four artificial stream channels, with four control channels receiving no antibiotics, and used cotton strips as a surrogate for leaves. Addition of antibiotics stimulated leaf litter decomposition, increased bacterial community diversity and altered bacterial community composition after 14 days, but …

Investigating The Quantity & Composition Of “Nurdles” At Micro & Macro Levels, Andrew Conover, Maya Worek, Olivia Schaul

Undergraduate Research and Engagement Symposium

Plastic pollution is a pressing global concern. Plastic pre-production pellets, or “nurdles” are widespread in the environment, but not yet well documented in rivers. In particular, nurdles are well documented along coastlines; additional efforts are needed to report the presence and concentration of these pollutants in freshwater habitats to fully understand the scope of the problem. This project had 2 objectives: 1) use community science (i.e., volunteer-generated) data to quantify nurdle abundance in rivers at a continental scale, and 2) measure the magnitude of the distribution of nurdles in a single tributary of the Chicago River.

Identification Of An Unknown Bacterial Species From A Sink Handle, Angelo Ricarte, Ahman Dye

Undergraduate Research and Engagement Symposium

This project aims to identify an unknown bacterial species isolated from a sink handle. Using a series of tests, the bacterium’s physical appearance, growth conditions, and metabolic characteristics will be examined. Its shape, colony appearance, and responses to different biochemical tests will help narrow down its identity. By comparing these results with the profiles of known bacteria, this project aims to determine which species is present and better understand the types of microbes commonly found on everyday objects.

Non-Alcoholic Fatty Liver Disease, Diet And Contributing Factors: A Narrative Literature Review, Rachel Joseph, Ashley Tharpe, Sherri Ann Walker

Wisdom & Compassion: The LUSON Journal

Objectives: The purpose of this narrative literature review is to explore the relationship between Non-alcoholic fatty liver disease (NAFLD) and dietary habits, and identify factors that contribute to the development, progression, and potential management of NAFLD.

Methods: Search terms such as non-alcoholic fatty liver disease (NAFLD), diet, and nutrition, including plant-based and vegetarian diets, were used to identify 45 peer-reviewed publications published within the last five years.

Results: Dietary factors, biological, environmental, obesity, mental health, and genetic factors can contribute to the development of NAFLD. This multisystem problem can have economic, social, and psychological consequences that affect a person’s quality …

Multi-Omics Systems-Guided Insights Of Withanolide And Other Bioactives Contributions To Anti-Stress And Endurance Outcomes In Withania Somnifera, Deepak Mundkinajeddu

Oxford ICSB

Conventional Ashwagandha standardization is largely focused on total withanolide quantification, without insights on the functional contribution of other phytochemical fractions. In this discovery-driven development program, orthogonal array–based model combined with predictive transcriptomics was used to delineate the relative biological roles of withanolide and other bioactive components within Withania somnifera extracts. Validated animal models showed that withanolide-enriched fractions significantly reduced serum cortisol (p< 0.05), while other bioactive-enriched fractions significantly increased run-time to exhaustion (p< 0.05), confirming anti-stress and endurance mechanisms. By linking the phytochemical profile with predicted biological pathway activity, the composition of the extract is rationally optimized for the development of a standardized extract (Ashwa.30), designed to deliver both anti-stress and endurance benefits at a dose of 30 mg. Clinical evaluation of the Ashwagandha extract demonstrated significant reductions in perceived stress scores and circulating cortisol levels, alongside measurable improvements in endurance parameters compared to placebo (p< 0.05), confirming translational alignment from preclinical discovery to human outcomes. Untargeted metabolomic profiling revealed enrichment of glycine and serine metabolism, methionine metabolism, phenylalanine and tyrosine metabolism, and homocysteine degradation pathways. These integrated metabolic changes worked together to improve energy production, support mitochondrial repair, maintain antioxidant balance, and help reset the body’s stress response system. This work presents a systems-biology–based approach to holistic Ashwagandha standardization, integrating withanolides and complementary bioactivities. It establishes a precision-driven framework for clinically validated adaptogenic efficacy.

Alkaloid Profiling Of Narcissus Poeticus L. Bulbs And Their Inhibitory Activity Of Cholinesterases, Maël Gainche, Isabelle Ripoche, Elodie Jagu

Oxford ICSB

The Amaryllidaceae family is well known for its rich diversity of alkaloids, including lycorine- and galantamine-type structures with potent cholinesterase inhibitory properties. In this study, we investigated the phytochemical composition and cholinesterase inhibitory activity of alkaloids isolated from Narcissus poeticus L., a species native to the botanically rich Auvergne region of France. Phytochemical analysis and bioguided fractionation of bulb extracts led to the identification of three known alkaloids, pseudolycorine, norgalantamine, and 2-O-(3’-hydroxybutanoyl)-lycorine, as well as a newly characterized tetrahydroquinoline derivative, poeticine A. The inhibitory activities of these compounds against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were assessed using both a standard …

The Erosion Of The Imago Dei: A Qualitative Study Of Religious Identity Maintenance Among Affluent Professionals In Secularized Environments, Shawn Hall

Liberty University Research Week

While sociological research has extensively documented the decline of religious affiliation (the "rise of the Nones"), fewer studies examine the erosion of theological anthropology, the fundamental beliefs about the nature of the self among religious adherents who remain active within faith communities. Drawing on twenty years of qualitative participant observation in the Washington, D.C., metropolitan area, this study investigates how affluent Christians maintain or fail to maintain a "Bipartite" (Soul-Body) worldview while embedded in highly secularized, technocratic professional environments. Utilizing a reflexive ethnographic design and NVivo-supported thematic analysis, the research identifies a phenomenon of "Ontological Dissonance," where adherents profess orthodox …

Temporal Variation In Dna Methylation Over 115 Years Post Introduction Facilitate Success Of House Sparrows, M. Ellesse Lauer, J. Dylan Maddox, Kevin D. Kohl, Mark Ravinet, Lynn B. Martin, Aaron W. Schrey

GS4 Student Scholars Symposium

House sparrows (Passer domesticus) have colonized most continents within the last 200 years. As such, they represent a unique study system for understanding the role that epigenetic mechanisms play in an individual’s responses to rapid environmental changes. DNA methylation facilitates the introduction success of non-native sparrows by compensating for reduced genetic diversity and supporting changes to phenotypes. In non-native populations of house sparrows, more recently introduced populations have higher variance in DNA methylation and respond to simulated infections with a greater magnitude of change in DNA methylation. To determine how DNA methylation changes temporally across 115 years post …

So You've Landed In A Fantasy World By Josiah Lebowitz, Nancy Martsch

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

So You've Landed in a Fantasy World by Josiah Lebowitz; book review.

Aubusson Weaves Tolkien: The Woven Adventure, Nancy Martsch

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Aubusson weaves Tolkien. The woven adventure/ Aubusson tisse Tolkien. L’aventure tissée: review of the catalogue of the exhibition held at the Cité Internationale de la Tapisserie in Aubusson, France, June 28 - September 30, 2024. The exhibition showcased 14 tapestries and 2 carpets based on Tolkien's illustrations. This catalogue is available in both French and English editions.

Evaluation Of Fasted And Fed Gastrointestinal Transformation Of Withania Somnifera Plant Extracts And Bioactive Compounds Via Uplc-Ms/Ms And Untargeted Metabolomics, Sarah Barr, Wendy Strangman, R. Thomas Williamson, Amala Soumyanath, Melissa Bollen

Oxford ICSB

Ashwagandha (Withania somnifera) is a widely consumed botanical supplement recognized for its anti-inflammatory, antioxidant, and anti-aging properties. Despite its extensive use, limited information exists on how its complex chemical composition changes during gastrointestinal digestion. An untargeted UPLC-MS/MS-based metabolomics approach enables analysis of discrete changes in complex mixtures. Previous in vitro studies of W. somnifera relied on simulated gastric and intestinal models composed primarily of buffers and digestive enzymes. This study advances those methods by incorporating bile salts and phospholipids under fasted and fed conditions to better simulate physiological digestion of aqueous and ethanolic extracts of W. somnifera root and leaf. …

Unmasking Ashwagandha Controversy: Safety, Toxicity, And Adulteration, Tahir Maqbool Mir

Oxford ICSB

Ashwagandha (Withania somnifera) is an adaptogenic shrub valued for its roots, which support systemic health. Increasing use has raised safety concerns about adulteration and hepatotoxicity. The roots of ashwagandha are primarily preferred for systemic health, while the leaves were traditionally reserved for topical applications to treat joint inflammation and promote wound healing.  Although generally considered safe, ashwagandha supplements have been linked to drug-induced liver injury, including cholestasis, jaundice, and pruritus. Reports from various organizations like LARB in the Netherlands indicate a rise in liver toxicity cases, possibly due to cytotoxic withafrin A and DNA-damaging withanone. A major concern is widespread …

Determinants Of Opioid-Relevant Exposure From 7-Hydroxymitragynine Products In The Evolving U.S. Marketplace, Igor Koturbash, Scott Alsbrook, George Pro

Oxford ICSB

Kratom’s U.S. market has rapidly evolved from largely botanical powders and capsules to products enriched in 7-hydroxymitragynine (7-OH), a potent mu-opioid receptor agonist that is typically present only at trace levels in leaf material. To quantify downstream behavioral health burden and contextualize it with real-world signals of product diffusion, we analyzed National Survey on Drug Use and Health (NSDUH) data (2021-2023) alongside public health alert trends and systematic online product surveillance. In NSDUH, 5.8% of adults met criteria for serious mental illness (SMI). SMI increased by 150% among adults with combined opioid use disorder (OUD) and kratom use between 2021 …

Relationships Among Emotional Intelligence, Emotional Reactivity, And Heart Rate Variability, Miriam Bowser

Liberty University Research Week

Ability emotional intelligence (EI) and trait EI have been correlated with heart rate variability (HRV), suggesting adaptive regulation facilitated by the autonomic nervous system. Emotional reactivity (ER) is also positively correlated with EI, possibly because individuals with higher EI are more sensitive to emotional information. The current study utilizes a cross-sectional design (N = 71) to measure trait and ability EI, self-reported emotional reactivity, and HRV. The current study found no relationships among trait and ability EI, suggesting that they encompass distinct underlying constructs. EI did not have a relationship with HRV, which could be due to the study’s small …

Fish Species And Diversity Database To Monitor Environmental Impacts Of Lake Hydaway Expansion, Lance Jones, Ella Mason, Sean Genota

Liberty University Research Week

Lake Hydaway’s recent expansion from 6 to 31 acres altered the habitat available for fish in the Opossum Creek watershed. This presents the opportunity to study the current fish populations and monitor changes in species composition and health over time. This long-term project seeks to create a standardized species database which will be used for various research purposes due to no such database or monitoring programs currently existing. Four representative sites of Opossum Creek were selected, two upstream and two downstream of Lake Hydaway. Each site was geolocated for reliable repeatability. Each site was sampled using backpack electrofishing following the …

Beyond Labeling: Examining Mpox As A Sexually Transmitted Infection- ¿A Literature Review¿, Rachel Williams, Ashlan Killeen, Eunice Onwuka

Liberty University Research Week

Introduction

Since 2022, Mpox outbreak trends have marked a shift in the epidemiology of the disease which has long been treated as a zoonotic infection. This is due to emerging patterns of cases of Mpox linked with sexual transmission, particularly in non-endemic regions reporting over 100,000 cases across 122 countries. This pattern has raised growing concerns regarding sexual transmission of Mpox necessitating further investigation.

Aim/Objective

This literature review examines current epidemiological, clinical/virological evidence to evaluate Mpox as an STI beyond labelling. and provides foundational context for a forthcoming systematic review examining priority populations affected during the 2022–2025 global outbreak.

Proposed …

Sensing Volatile Organic Compounds Via Photonic Crystals Of The Morpho Zephyritis Butterfly, Parker Abila

Liberty University Research Week

No abstract provided.

Active Barrier Cream Against Poison Ivy (Urushiol), Amanda Witherell, Annalee Gardner, Luthien Jones

Liberty University Research Week

Urushiol is an immunotoxic compound composed of a 3-substituted catechol and an alkyl side chain that contains 0-3 degrees of unsaturation. It is the substance in poison ivy that causes a negative response when it contacts skin. Commercial barrier creams currently exist that claim to protect the skin against urushiol but only serve to form a barrier between the oil and skin. This project aims to create a barrier cream that both forms a physical layer to protect the skin and neutralizes the urushiol as it sits on the cream. To neutralize the urushiol, it is proposed that either the …

Computer Vision For Ovarian Follicle Analysis, My Nhien Tang, Cameron Kauffman, Ian Davidson, Jumana Rizk, Maria Mendoza Lopez, Noam Kimbro, Tan Loc Nguyen, Will Starling

Liberty University Research Week

The present study investigates the ability of computer vision technology to count primordial follicles in the primate ovary. The number of primordial follicles, i.e., ovarian reserve, reflects the ovary’s potential of supporting endocrine and reproductive function in females. Follicle counting has been used in translational and clinical studies to assess ovarian health and response to regulatory factors or toxic substances. This critical process is currently performed manually by experienced laboratory personnel. Existing research on computer vision software’s ability to count follicles has yielded limited results. Therefore, in reaction to the labor-intensive, time-consuming nature of manual follicle counting and technological growth …

From Exposure To Detection: Health System Barriers To Diagnosing Asbestos-Related Diseases In Developing Countries, Matthew-Riece Castanares, Luke Montgomery

Liberty University Research Week

No abstract provided.