Digital Commons Network^™

Adaptation And Utilization Of Three Legume In China, Huang Wenhui

IGC Proceedings (1977-2023)

China is a vast territory with extremely varied climates, soil, and topography, and it is very rich in pasture grass resources. The main legumes for forage are Alfalfa (Medicago sativa L.), yellow alfalfa (Medicago folcata L.) and shadawang (Astragalis adsurgent Pall.)

Preliminary Evaluation Of Legume Germ Plasm In The Cerrados Of Brazil, D Thomas, R P. De Andrade

IGC Proceedings (1977-2023)

In 1978 an evaluation program was initiated to select legumes that (1) grow and produce seed on acid soils under aluminum and water stress, (2) persist under grazing, and (3) tolerate pests and diseases. The work is being conducted at the Cerrados Agricultural Research Center, near Brasilia, at lat 15°S and altitude 1,000 to 1, 100 m . Annual rainfall is 1,500 mm, mostly distributed in a 6-month wet season, and mean annual temperature is 21°C. Nine hundred legume accessions from thirteen genera have now been established as spaced plants on the 2 major soil types of the region. The …

Genetics And Utilization Of Apomixis For The Improvement Of Guineagrass (Panicum Maximum Jacq.), Y H. Savidan

IGC Proceedings (1977-2023)

Natural polymorphism in guineagrass (Panicum maximumjacq.) has been stabilized in tetraploid biotypes by apomixis. Sex­ual diploids were found in East African populations. Our objective was to determine whether apomixis could be related with a simple genetic background and manipulated in an improvement program. Apomicts of guineagrass are characterized by a 4-nucleate embryo sac structure, and this trait was used in genetical analyses to differentiate apomictic from sexual hybrids. Progeny tests were also conducted as controls. Ten different types of progenies were analyzed: tetraploid sexual (from colchicine treatment of diploids) x apomictic F1 hybrids; selfed and backcrossed progenies of sexual F1 …

Breeding Challenges In Apomictic Warm-Season Grasses, E C. Bashaw, P W. Voigt, B L. Burson

IGC Proceedings (1977-2023)

Efforts to breed apomictic grasses met with little success in the past, but results of more recent research show significant progress with some species and new prospects for improving apomicts. Early research uncovered the cytological basis for apospory and diplospory, found the common apomictic mechanisms in grasses, and showed that apomixis is transmitted from pannt to offspring. Discovery of sexual or partially sexual (facultative apomicts) plants in several species formerly considered obligate apomicts allowed for hybridization and genetic studies. Results of subsequent research confirmed inheritance of apomixis, and data from the more conclusive studies indicate that method of reproduction …

The Role Of Artificial Intelligence In Marine Pollution: A Review, Safaa A. Abdel Ghani, Sahar W. M. Hassan, Aida H. Shobier

Blue Economy

Marine pollution represents a significant threat to the sustainable use of ocean resources and is increasing globally. Consequently, multidimensional and sustainable ocean governance frameworks have placed growing emphasis on the prevention, reduction, and elimination of marine pollution. In recent years, artificial intelligence (AI) has been increasingly adopted as an effective tool for monitoring, assessing, and managing marine pollution. This review aimed to highlight the role of artificial intelligence in addressing marine pollution. The efforts of scientists in employing and leveraging the advantages of AI to tackle the issue of marine pollution were examined. It was found that AI, supported by …

Improving The Science Curriculum: A Uae Middle School Case Study, Fatima Husain

Journal of Curriculum, Teaching, Learning and Leadership in Education

United Arab Emirates Public Schools in Abu Dhabi implement curricula developed by the Ministry of Education (MOE). This qualitative case study research aimed to explore the perspectives of both teachers and students regarding the obstacles impacting student academic achievement in Science classes. The study was conducted in one middle school in Abu Dhabi, EARTH School (pseudonym) that faced challenges in the first trimester of the 2023 academic school year. The students identified teacher quality and well-being as obstacles and suggested improving student motivation and teaching strategies. The teachers identified professional development and student language barriers as core issues and suggested …

European Corn Borer Management In Field Corn, Igor Sulzbacher Schardong, Francis Reay-Jones

Agronomic Crops

In the past, European Corn Borer (ECB) was considered a billion-dollar pest. Bt corn was first released in 1996 and its widely adoption reduced ECB populations and economic importance. Resistance to some Bt toxins was detected in Canada, representing a possible threat to corn production. In this paper, we reviewed potential management tactics for ECB in corn.

“It's Because Of Me – I Made Myself Get Here”: First-Generation College Students In Stem Motivations For, And Experiences With, Academic Advising, Desiree Forsythe, Amy C. Moors, Quaylan Allen, Jeremy L. Hsu

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Academic advising plays a major role in shaping undergraduate life sciences students’ experiences through college. However, despite the critical nature of advising, there have been relatively few studies examining students’ experiences with advising and how these experiences may influence their academic journeys. Here, we investigate first-generation college students’ experiences with academic advising in biology and throughout STEM. Through the lens of community cultural wealth, we found several themes such as balancing independence and pressures to succeed, identity as a source for empowerment and motivation, wanting reassurance and support from advisors, as well as negative outcomes during interactions with advisors. We …

Visualizing Ozone Pollution In The Denver Metro Area Using A Bioindicator Garden, Ella K. Mathews, Hillary Hamann, Julie Morris, Erica L. Larson

DU Undergraduate Research Journal Archive

Since the industrial revolution, widespread fossil fuel combustion has significantly increased atmospheric pollution. The combustion of fossil fuels releases nitrogen oxides (NOx) and volatile organic compounds (VOCs), which form tropospheric (ground-level) ozone when exposed to sunlight. Ozone directly harms plants by disrupting their ability to absorb carbon dioxide, release oxygen, and carry out photosynthesis. Bioindicator species are plants that have visible responses to environmental conditions, such as ozone, and can act as “canaries in the coal mine,” indicating the health of their environment. In this study, we used a garden of ozone bioindicator species (ozone garden) as a cost-effective tool …

Mothers On The Ballot: Gender And Partisan Dynamics In The Evaluation Of Maternal Political Candidates, Jimena Sanchez

DU Undergraduate Research Journal Archive

While scholarly research on the interaction between gender and political candidacy has grown, there is limited research on the way mothers are perceived in politics when they choose to run for office. The goal of the paper is to answer the following research question: how do partisanship and gender influences public perceptions of mothers running for political office, and to what extent does social desirability shape these attitudes? Through a list experiment, I surveyed individuals in the United States to capture the beliefs of the public toward mothers running for political office. Specifically, I analyze the opinions of survey participants …

Dr. Jonathan Velotta Faculty Spotlight, Katelyn Trimble

DU Undergraduate Research Journal Archive

An interview with Dr. Jonathan Velotta of the University of Denver's Department of Biological Sciences.

Du Undergraduate Showcase Abstracts: Research, Scholarship, And Creative Works, Sophia Wismar, Henry Staats, Allison Metzler, Chloe Puckett, Rachel Levine, Christa Kilpatrick, Scott Wolf, Joe Walsh, Grace Doolittle, John Engebreston, Zoe Lopez, Christopher Aaby, Audrey Duff, Timothy Sisk, Katelyn Lamberton, Angela Narayan, Gilkah Argueta, Habiba Samir, Girena Tesfazghi, Genet Kenore, Sinit Tesfamariam, Effley Brooks, Abi Newell, Megan Doherty, Natalie Baer, Lexi Blood, Talya Riciputi, Jessica Jimenez, Devin Hernandez, Lynn Clark, Taj Kumar, Sunil Kumar, Allen Rutman, Mira Pronobis, Tess Carson, Anna Sher, Frankie Stroud, Tamra Pearson D'Estree, Alyssa Wilson, Emily Melnick, Jenalee Doom, Yihang Gao, Gwendolyn Geiger, Noah Gettle, Scott Nichols, Clare Ayoub, Cara Dienno, Sunny Walker, Zoe Hansen, Maya Wheeler, Addison Rice, Patrick Martin, Sanjana Acharya, Daniel Mcintosh, Amanda Mckellips, Calli Cain, Justin Blake, Peter Sokol-Hessner, Natalie Miller, Max Weisbuch, Sophia Dellota, John Macikas, Charlotte Snow, Mark Siemens, Zoe Lynch, Alex Huffman, Prachi Shah, Jason Roney, Halcyon Levi, Nicole Herzog, Andrea Koly, Daniel Linseman, Annie London, Xi Yang, Avery Zwisler, Jane Smith, Chaz Contag, Michael Kerwin, Lucy Rand, Grace Schroeder, Michelle Rozenman, Nissa Tapper, Guiming Zhang, Mateo Mazariego-Halpern, Keith Meyer, Julie Do, Dakota Park-Ozee, Travis Herink, Kara Neu, Jonathan Plomin, Eve-Odine Duchaufour, Debbie Gale Mitchell, Tennyson Anderson-Stricklin, Lily Treitz, Samantha Rosenberger, Sierra Griffith, Finley Joseph, Daniel Sampson, Emmy Davis, Skyler Kasnoff, Evon Lopez, Vivian Nguyen, Cassy Young, Franklin Sellner, Martin Tobon, Ila Graham, Zach Billings, Holden Hedit, Decatur Boland, Paul Kosempel, Cory Chandler, Jay Mahoney, Sam Dragan, Susan Dagget, Yarrow Ator, Heidi Vuletich, Owen Weber, Andrew Kloeppel, Petersen Gray, Mandi Schaeffer-Fry, Razleen Bassra, Bryanna Rodriguez, Christina Blue, Taubie Sanders, Rachel Epstein, Luke Milburn, Camryn Evans, Ezra Martinez, Mary Westwood, Gabri Notov, Robin Tinghitella, Lilou Cabrol, Eli Barbour, Juliet Mendik, Selma Myers, Zac Wise, Noah Fahlin, Michelle Knowles, Abigail Hopper, Michael Greenberger, Romi Laclair, Sarah Watamura, Sabrina Efroymson, Casey Barker, Sydney Seltzer, Bryn Yehle, Jennifer Hoffman, Sara Garcia, Ryuka Nagamine, Trevor Briggs, Remy Le Boeuf, Elena Krone, Eileen Farrell, Regan O'Rourke, Elena Roel, Greg Mortimer, Ali Ayoub, Stefani Langehennig, Caitlin Turk, Logan Scmid, Stefan Chavez-Norgaard, Karen Kim, Tatiana Peccedi, Courtney Cassidy, John Sebesta, Rhianna Lewis, Janice Bening-Lacek, Vivian Lawless, Mckenna Hanson, Jeffrey Amidon, Riya Joshi, Ram Ambre, Brady Worrell, Perrin Schneider, Ali Azadani, Brooke Agulnek, Lyndsie Salvagio, Elise Siemanowki, Yan Qin, Andre Allen, Melodie Nguyen, Megan Livengood, Abby Reams, Saffron Hartreeve, Bri Wylie, Sarah Brookman, Mariah Loiacono, Green Russo, Abhia Lodhi, Gabrielle Welsh, Nika Spehar, Shahked Levin, Evrim Baykal, Kimberly Chiew, Jocelyn Torres, Kailey Hicks, Mykaela Tanino-Springsteen, Audrey Bellows, Akam Chahal, Madeline Tepper, Shannon Murphy, Alexa Fonseca, Deborah Han, Cassandra Perez, Oluwatoyin Alaba, Julia Roncoroni, Vy Nguyen, Nana Burn, Sarah Sasse, Rubin Tuder, Anthony Gerber, Nancy Lorenzon, Christine Vohwinkel, Camryn Gunter, Tristan Weber, Sam Rommel, Brian Michel, Muskan Fatima, Alannah Oleson, Kira Frey, Edward Garrido, Beckett Morris, Kerstin Haring, Drew Middleton, Abigail Walpert, Liam Dee, Gabby Ishaw, Cole Carnes, Maddie Weiser, Claire Fox, Valeriia Vlasenko, Kateri Mcrae, Riley Smith, Abigail Templin, Kushani Rajapaksha

DU Undergraduate Research Journal Archive

Abstracts from the DU Undergraduate Research Showcase.

Patron-Client Relationship Undermines Sustainable Fishery Governance: A Case Of Small-Scale Fishing Certification In Sulawesi, Indonesia, Desiree Simandjuntak

Forest and Society

One of the ways market-based fishery governance attempts to boost sustainable fishing is through economic incentive. Offered as an above-market price to producers, it is commonly practiced in seafood certification to encourage sustainable and equitable production. However, certification is mainly inaccessible to small-scale fishers who are typically tied to pre-existing patron-client relationship, particularly those from developing economies. This study analyzes the extent to which economic incentive can benefit small-scale fishers amidst the intricate power imbalance in producer community. Based on a ten-month ethnography study in Sulawesi, Indonesia, it finds that prevalent patronage impedes participating fishers' income as it is affected …

Environmental Ethics And The (Dis)Continuity Of Meaning In Experience And Nature, Harrison S. Jackson

Dewey Studies

No abstract provided.

Panda-Plus-Bench: A Clinical Benchmark For Evaluating The Robustness Of Ai Foundation Models In Prostate Cancer Diagnosis, Joshua L. Ebbert, Dennis Della Corte

Faculty Publications

Artificial intelligence foundation models are increasingly deployed for prostate cancer Gleason grading, where GP3/GP4 distinction directly impacts treatment decisions (active surveillance vs. intervention). However, these models may achieve high validation accuracy by learning specimen-specific artifacts rather than generalizable biological features, limiting real-world clinical utility. We introduce PANDA-PLUS-Bench, a curated benchmark dataset derived from expertly annotated prostate biopsies designed specifically to quantify this failure mode. The benchmark comprises nine carefully selected whole slide images from nine unique patients containing diverse Gleason patterns, with non-overlapping tissue patches extracted at both 512 × 512 and 224 × 224-pixel resolutions across eight augmentation conditions. …

Place-Based Education: A Rural Solution For Student Achievement, James A. Chamness

College of Education

Rural secondary students in the United States continue to demonstrate lower academic outcomes on standardized assessments compared to their non-rural peers, highlighting the need for contextually responsive instructional approaches. This concurrent mixed methods study examined the relationship between place-based education (PBE) and academic achievement among rural secondary students and explored rural teachers’ perceptions of PBE. Guided by social cognitive theory, the study investigated differences in ACT composite scores between students exposed to PBE and those receiving traditional instruction, as well as perceived challenges and opportunities associated with PBE implementation. Quantitative data included archived ACT scores, which were analyzed using descriptive …

Backscattering By Particles Of Sizes <0.2 Μm In Seawater, Xiaodong Zhang, Yuanheng Xiong

Faculty Publications

We measured the angular scattering functions at 517 nm in different size fractions, including bulk and very small particles (VSP for diameters <0.2 μm), in a variety of waters ranging from coasts to open oceans and from surface to depths as deep as 3,000 m. The measurements revealed a strong covariation in backscattering between VSP and bulk particle populations. The fractional contribution by VSP decreases from 60% in clear oceanic water where bulk particle backscattering coefficient bbp is on the order of 0.001 m⁻¹ to <2% in turbid coastal water where bbp ∼0.1 m⁻¹. An empirical model is developed (r² = 0.89) to estimate bbp for VSP from the bulk bbp, which can now be routinely obtained from satellite observations.

Lovastatin Potentiates The Function Of Α7-Nicotinic Acetylcholine Receptors, Dmytro Isaev, Keun-Hang Susan Yang, Murat Oz

Pharmacy Faculty Articles and Research

Background/Objectives: Statins are currently one of the most commonly used cholesterol-lowering drugs. In recent years, in addition to their well-known effects on the cardiovascular system, statins have been shown to exert beneficial effects in the progression of various neuropsychiatric and neurodegenerative diseases. Methods: In this study, the effects of lovastatin on the function of α7-nicotinic acetylcholine (nACh) receptors expressed in rat hippocampus and Xenopus oocytes were investigated. Results: In whole-cell patch clamp studies in hippocampal neurons, 21-day chronic (20 mg/kg), but not acute (20 min), lovastatin treatment caused significant potentiation of choline (a selective agonist for α7 nACh receptors)-induced currents …

Integrating Local Ethnobiology Into Sustainable Forest City Development In Indonesia's New Capital City, Tri Atmoko, Mukhlisi Mukhlisi, Bina Swasta Sitepu, Ulfah Karmila Sari, Eko Herwanto, Deny Adi Putra, Tri Rizkiana Yusnikusuma

Forest and Society

The Nusantara new capital city has been a national strategic program based on Indonesian's sustainable forest city concept. In the future, there will be a migration of 1.9 million people from different islands, indigenous, and religions, carrying their own cultures. It will suppress the existence of local cultures. Local culture lives in harmony with nature, but massive development will impact the presence of forests and their culture. This research aims to document various local cultures related to ethnobiology (plants and wildlife) of the indigenous in the Nusantara region and its surroundings (Dayak, Paser, and Kutai). The research was conducted through …

A Testis-Specific Aralkylamine N-Acetyltransferase Regulates Dimorphic Sperm Function And Male Fertility In Moths, Hao Sun, Peng Yi Huang, Zhi Ruo Zhang, Cong Fen Gao, Subba Reddy Palli, Shun Fan Wu

Entomology Faculty Publications

Most Lepidopterans produce two distinct sperm types—nucleated eupyrene (fertile) and anucleate apyrene (non-fertile), yet the genetic mechanisms governing this dimorphism remain poorly understood. Using the fall armyworm (Spodoptera frugiperda) as a model, we identified a Lepidoptera-conserved, testis-specific arylalkylamine N-acetyltransferase (LTNAT) indispensable for male fertility. Functional analyses showed that LTNAT is expressed in both sperm types, and its depletion disrupts mitochondrial derivatives (MDs) in eupyrene sperm and drastically impairs apyrene sperm motility. Multi-omics analyses revealed LTNAT mutation perturbs energy metabolism, lipid homeostasis, and transmembrane transport—alterations correlating with abnormal MD structure. Furthermore, LTNAT deficiency likely compromises apyrene sperm motility by downregulating Sex-lethal …

Unlocking The Colleague Next Door, Yoshihiko Ariizumi

Learning, Teaching, & Researching Optimization

The structural barriers that isolate faculty from one another—individualistic reward systems, professional identity protection, disciplinary silos, fragmented time—have been documented in detail elsewhere. This paper begins where that analysis ends. Its sole focus is solutions: what actually works, what you can do without waiting for your institution to change, and most importantly, what you can do today. It is organized in three movements: evidence-based institutional strategies, community-level structures any group of faculty can build, and a granular map of the smallest possible individual first steps—the ones that cost almost nothing and have an outsized probability of changing how you experience …

Food Traceability Rule Vs. Produce Safety Rule, Hema Lingireddy, Amanda J. Deering, Tari Gary, Scott Monroe, Katheryn Parraga, Rama Radhakrishna

Purdue Extension Numbered Publications

The federal Food Traceability Rule and Produce Safety Rule address different but complementary aspects of food safety. In this publication, Purdue University researchers support understanding and clarification by explaining the differences and how the rules work together within the Food Safety Modernization Act. This publication pairs with FS-73-W and FS-74-W.

Target Trial Of Glucagon-Like Peptide-1 Agonists Versus Dipeptidyl Peptidase-4 Inhibitors And Mortality In Hemodialysis, Dustin Le, Duncan Kilpatrick, Walter K. Kraft, Morgan E. Grams, Bernard G. Jaar, Jung-Im Shin

Division of Nephrology Faculty Papers

INTRODUCTION: The comparative effectiveness of glucagon-like peptide-1 (GLP-1) agonists (GLP-1s) versus dipeptidyl peptidase-4 (DPP-4) inhibitors (DPP-4s) in hemodialysis is unknown.

METHODS: Using 2011 to 2021 US Renal Data System and Medicare claims data, we emulated a target trial comparing new users of GLP-1s versus DPP-4s with patients who survived ≥ 90 days on in-center hemodialysis before medication initiation. We used inverse probability of treatment weighting (IPTW) and Cox proportional hazards models to estimate hazard ratios (HRs).

RESULTS: We identified 3671 GLP-1 and 12,039 DPP-4 users. After IPTW, mean age was 63 years, 52% male, 63% White, and mean body mass …

Plant-Derived Bioactives, The Gut-Brain Axis, And Neurodegenerative Diseases: Mechanistic Roles Of Diet-Microbiota Interactions, Ashley Reynolds, Ethan Glenn, Brigitte Lavoie, Suzanne L. Ishaq, Yanyan Li

Pharmacy Faculty Scholarship

Diet is increasingly recognized as a potential upstream modulator of the gut-brain axis (GBA) through its effects on the microbiome, microbial metabolites, and host immune and endocrine responses. The GBA is a complex, bidirectional network connecting the gastrointestinal tract and central nervous system, with diet influencing microbial community structure and metabolic output. Plant-based diets, such as Mediterranean and MIND, have been associated with increased production of anti-inflammatory microbial metabolites and improved barrier function, while high calorie/low nutrient diets are often linked to increased immune activation and barrier dysfunction. However, while microbial metabolites, especially short-chain fatty acids, indoles, bile acids, and …

Structure Of Ergosteryl-Aspartate Synthase Reveals How An Entrapped Trna Is Used Like A Prosthetic Swinging Arm In The Synthesis Of Aminoacylated Sterols, Hanako Murayama, Nathaniel Yakobov, Nassira Mahmoudi, Sasha Legrosdidier, Nicolas Fournier, Solène Zuttion, Kanata Matsumoto, Michihiro Nishimura, Jingwei Ji, Bruno Senger, Laurence Huck, Howard Gamper, Yoshiaki Kise, Ralph Kleiner, Mathieu Frechin, Ya-Ming Hou, Hubert Becker, Yuzuru Itoh, Frédéric Fischer, Osamu Nureki

Department of Biochemistry and Molecular Biology Faculty Papers

Ergosteryl-3β-O-L-aspartate synthase (ErdS) catalyzes tRNA-dependent aspartylation of ergosterol, a lipid essential for fungal cell membrane integrity. However, the functional significance of ergosteryl-aspartate and the molecular mechanisms underlying its synthesis remain unclear. Here, we show that ErdS localization is highly dynamic and that Erg-Asp is required for proper hyphal growth, sporulation, and spore germination, and likely influences stress tolerance. The cryo-electron microscopy structure of ErdS revealed an unprecedented sterol-binding pocket. In addition, the structures in complex with a non-hydrolyzable Asp-N-tRNAAsp show a tRNA-guided intramolecular aminoacyl transfer mechanism between two functional domains of the enzyme. The CCA end of tRNAAsp undergoes a …

Movement, Residency, And Behavioral Plasticity Of Reef Manta Rays In The Samarai Islands Of Papua New Guinea, Anna Knochel, Joanna Harris, Shannon Murphy, Annie Murray, Guy M.W. Stevens, Mark Erdmann

School of Biological and Marine Sciences

The reef manta ray (Mobula alfredi) is a highly mobile pelagic marine ray found throughout the tropical and subtropical waters of the Indo-Pacific, but investigation into their behavior and ecology within Papua New Guinea has not been previously undertaken. Furthermore, the home range, dispersal characteristics, and inter-seasonal fine-scale habitat use of this species is limited. To address these data gaps and investigate the vertical and horizontal habitat use of a previously unstudied population, SPLASH10-F-321A pop-off archival satellite tags were used to track ten adult individuals from 4–181 days between 2016–2018 across two distinct monsoonal periods in the Samarai Islands of …

An Ecological Survey Of Algal Species In The Sawyer River, Al-Muthanna Governorate, Iraq, Rasha.H. R. Al-Miawi, Hiafaa A.J. Al-Barakat, Alaa Jabar Mahmoud

Muthanna Medical Journal

Given the global changes in climate, especially the drought conditions Iraq has recently experienced, along with the severe water shortage, this study sought to develop both quantitative and qualitative methods and strategies for phytoplankton (excluding diatoms) as key indicators to protect ecosystems, boost their resilience, and promote sustainability. sites were chosen for the most prominent cities A number of samples were selected from three sites (Al-Dahara region, Al Jarukhi Bridge, Al-Ghanim area), located on the Al-Sawyer River, which is the most important branch of the Euphrates River in the Al-Muthanna Governorate in southern Iraq in 2024 the species that was …

Icam1high Neutrophils Sculpt Tumor Evolution And Metastasis Through Symbiotic Adhesion And Reverse Migration, Ling Wu, Zhan Xu, Jun Wang, Fengshuo Liu, Yunfeng Ding, Yang Gao, Siyue Wang, Jun Liu, Hilda L Chan, Weiguo Wu, Yi-Hsuan Wu, Liqun Yu, Xiaoxin Hao, Xuan Li, David G Edwards, Cher Sha, Tobie D Lee, Nan Guan, Sergio Aguirre, Luis Becerra-Dominguez, Dane Hoffman, Xiang Chen, Charlotte Helena Rivas, Xiaoyuan G Yan, Igor L Bado, Weijie Zhang, Qian Zhu, Arun Sreekumar, Robert L Satcher, Xiang H-F Zhang

Faculty, Staff and Students Publications

Neutrophils are a prominent component of the tumor microenvironment that can have both pro- and anti-tumor functions. By analyzing neutrophils across different human cancers, we revealed an ICAM1high subset enriched in the tumor microenvironment, which were also observed in murine triple negative breast cancer (TNBC) models. ICAM1high neutrophils exhibited an enhanced capacity for cell-cell adhesion specifically with tumor cells retaining epithelial features, and this adhesion conferred mutual advantages to both cell types. In contrast, cancer cells with mesenchymal-like phenotypes were vulnerable to neutrophil-mediated cytotoxicity due to decreased cell adhesion and elastase resistance. These opposite effects drove tumor evolution toward a …

Development Of A Second-Generation Rarα Selective Antagonist As An Orally Bioavailable, Effective, Safe, And Reversible Male Contraceptive, Rui Shi, Kristen John, Xuan Qin, Ehfazul Haque, Taimeng Liang, Narsihmulu Cheryala, Feng Li, Henry L Wong, Gunda I Georg

Faculty, Staff and Students Publications

We report the design and SAR studies of benzopyran-, benzofuran-, and benzothiophene-derived inhibitors of the retinoic acid receptor alpha (RARα) for male contraception. SAR studies identified critical features influencing activity, such as the optimal positioning of antagonism moieties and substituents, leading to the discovery of (S)-4-(5-(2,8-dimethyl-5-(p-tolyl)-2H-chromen-3-yl)-1H-pyrrol-2-yl)­benzoic acid (compound 23). Compound 23 is a highly potent RARα inhibitor (IC50 = 0.051 nM) with excellent selectivity (>1650-fold over RARβ and >1960-fold over RARγ) and ADMET properties. Compound 23 is orally bioavailable and reduces sperm counts in mice for a full male contraceptive effect …

Unbiased Niche Labeling Maps Immune-Excluded Niche In Bone Metastasis, Zhan Xu, Fengshuo Liu, Yunfeng Ding, Tianhong Pan, Yi-Hsuan Wu, Yujiao Han, Jun Liu, Igor L Bado, Weijie Zhang, Ling Wu, Yang Gao, Xiaoxin Hao, Liqun Yu, Xuan Li, David G Edwards, Hilda L Chan, Sergio Aguirre, Michael Warren Dieffenbach, Elina Chen, Siyue Wang, Yichao Shen, Dane Hoffman, Luis Becerra Dominguez, Charlotte Helena Rivas, Xiang Chen, Hai Wang, Yibin Kang, Zbigniew Gugala, Robert L Satcher, Xiang H-F Zhang

Faculty, Staff and Students Publications

Metastatic cancer cell fate is shaped by the local microenvironment niches. To unbiasedly define the cellular and molecular features of metastatic niches, we developed sortase A-based microenvironment niche tagging (SAMENT), which selectively labels cells encountered by cancer cells during metastasis. Applying SAMENT across multiple cancer models and target organs revealed shared niche features, including macrophage enrichment and T cell depletion, alongside marked organ-specific phenotype heterogeneity in niche macrophages. In bone, metastatic niches are enriched for macrophages expressing estrogen receptor alpha (ERα) with active ERα signaling. Conditional deletion of Esr1 in macrophages significantly impaired bone colonization by enabling T cell infiltration. …