Digital Commons Network^™

The Effect Of Rider Balance On Equine Heart Rate During Therapeutic Riding, Lydia R. Nelson

Undergraduate Theses and Capstone Projects

Therapeutic riding provides emotional and physical benefits for riders who have disabilities, yet the physical demands placed on equines in therapeutic riding are not fully understood. Equestrian imbalance is a common characteristic in therapeutic riding, which may increase the workload on the equine. This study aims to evaluate whether induced rider imbalance increases equine heart rate, indicating greater workload, in comparison to riding with a balanced equestrian. One therapeutic riding equine and one experienced equestrian from Brook Hill Farm participated in this study. Heart rate (HR) data were collected with a heart rate monitor strapped around the equine for 21 …

Together In Sync: Assessing Prosocial Behavior With A Full-Body Interactive Mixed Reality Experience, Sena Beste Ercan, Belen Gonzalez, Olga Gali, Gray Atherton, Liam Cross, Narcis Pares

School of Psychology

This study investigates how interpersonal synchrony (IPS) in full-body interactive Mixed Reality (MR) environments can shape children's prosocial behavior. We introduce DragonIce 2.0, a two-part MR game designed to induce synchronous or asynchronous group activity and assess their impact on prosocial behavior. To examine changes in affiliation (team belonging and peer liking) and collaboration (overt joint actions), we conducted a comparative study with children aged 9–10, using both system logs and self-report measures. The results highlight the importance of context, task structure, and perceived coordination in shaping social outcomes, offering insights into how interaction dynamics shape social engagement in immersive …

Criminalizing Curation, Duncan Hosie

William & Mary Bill of Rights Journal

This Article surfaces emerging threats to artistic expression posed by the Dost test, which outlines six factors for interpreting a “lascivious exhibition of the genitals” under federal and many state child pornography laws. It argues that the present state of American politics has eroded normative inhibitions that previously prevented the ambiguities and vulnerabilities of child pornography prosecutions from being systematically exploited. By way of repair, the Article offers two jurisprudential reform proposals.

Synthesizing precedent, criticism, and theory, the Article demonstrates Dost invites selective enforcement against politically and religiously unpopular artwork by authorizing courts and prosecutors to assess polysemous visual works …

The End Of The List: Constructive Waiver Of The Right To Counsel In Virginia, Thomas C. Squires

William & Mary Bill of Rights Journal

This Note will first give a general background on the right to counsel in the United States that stems from the Sixth Amendment of the U.S. Constitution, with a focus on the right as applied in the Commonwealth of Virginia. A brief examination of the specific Virginia Code section that is relevant when discussing this issue will follow. This code section and its deficiencies collectively present potential threats to defendants’ right to counsel. An evaluation of federal case law and Virginia case law on constructive waiver will follow. This Note will conclude by offering potential solutions to head the looming …

Versatile Exoskeleton Control Paradigms For Agile Human Locomotion: Task-Agnostic And Stability-Augmented Assistance, Miao Yu

All Dissertations

Lower-limb exoskeletons have shown great potential for assisting human locomotion, but designing controllers that provide assistance across diverse locomotor tasks and under external perturbations remains a major challenge. Many existing controllers for steady-state walking rely on pre-defined reference trajectories, which constrain voluntary human motion and limit adaptability across tasks. Moreover, they usually assume stable walking, while their performance under unstable conditions remains largely unexplored. In addition, existing gait stability augmentation controllers are often reactive rather than proactive to unstable conditions. In this dissertation, we propose control frameworks that preserve voluntary human motion while addressing two major challenges: providing task-agnostic assistance …

Optimized Ultrasound Imaging Of Phase-Change Nanodroplets, Charles R Dyall, Dmitry Nevozhay, Andy Liu, Trevor M Mitcham, George J Lu, Richard R Bouchard

Faculty, Staff and Student Publications

Phase-change nanodroplets (PCNDs) continue to generate significant research interest due to their potential to extravasate into tissue, to be targeted for molecular imaging and drug delivery, and to undergo an induced phase-change to "activated" microbubbles (MBs) for ultrasound (US) imaging. To accurately quantify molecular markers, however, one assumes a consistent proportion of PCNDs in a region of interest (ROI) are stably activated and imaged. Herein we present a framework for developing a diagnostic sequence that is optimized for PCND activation uniformity, contrast, and acquisition time. To develop this framework, activation was examined at three scales of increasing complexity: single, adjacent, …

Potential Impact Of Parasites In The Transmission Of Chronic Wasting Disease, Paulina Soto, Rodrigo Morales

Faculty, Staff and Student Publications

No abstract provided.

Flicking Fibres: Microfibres Act As Sensory Disruptors In A Marine Crustacean, Ari Drummond, Alexander D.M. Wilson, Lucy M. Turner, Mark Briffa

School of Biological and Marine Sciences

Anthropogenic inputs to the environment—including microplastics and microfibres—are global stressors known to impair multiple facets of organismal biology. However, the effects of these pollutants on sensory structures remain critically understudied. Sensory systems mediate organism-environment interactions, and pollution-derived impairment at these interfaces may cascade through bioprocessing, from information acquisition to behaviour and ultimately fitness. Microfibres, in particular, pose an additional threat, potentially physically obstructing sensory organs and chemically interfering with sensory processes. Using an invertebrate model of sensory biology, Pagurus bernhardus, we tested whether dryer lint—a complex, heterogeneous mix of treated microplastic and cellulose fibres, and other anthropogenic contaminants—acts as a …

Are Drains After Trauma Splenectomy Associated With Organ Space Surgical Site Infections?, Stephanie Martinez Ugarte, Mokunfayo O Fajemisin, Seokhun Kim, William D Rieger, Renee W Green, Gabrielle E Hatton, Lillian S Kao

Faculty, Staff and Student Publications

Introduction: Left upper quadrant (LUQ) drain use after trauma splenectomy varies, with minimal existing data to guide practice. We aimed to investigate the association of LUQ drains with organ space surgical site infection (OS-SSI) after splenectomy. We hypothesized that LUQ drains after trauma splenectomy are associated with increased odds of OS-SSI.

Patients and methods: A retrospective study of adult blunt trauma patients who underwent splenectomy from 7/2018 to 6/2024 was performed. We excluded patients who survived < 5 days after operation, and those with an intraoperatively identified pancreatic injury. Demographics, surgical details, and drain information were abstracted from the medical record. Inverse probability of treatment weighting (IPTW) was used to balance variables between patients who did and did not receive an LUQ drain after splenectomy. Multivariable logistic regression with IPTW was performed to estimate the association of LUQ drains with OS-SSI.

Results: Of 179 patients, 68% (n = 121) were male, the median age was 38 years (interquartile range 28, 55), and 27% (n = …

Computational Modeling Meets 3d Bioprinting: Emerging Synergies In Cardiovascular Disease Modeling, Tanmay Mukherjee, Mehdi Salar Amoli, Sarah Rezapourdamanab, Lama Rita El Shammas, Martin L Tomov, Emilio A Mendiola, Vahid Serpooshan, Reza Avazmohammadi

Faculty, Staff and Student Publications

Cardiovascular diseases (CVDs) remain the leading cause of death worldwide, underscoring the need for improved strategies in diagnosis, treatment, and disease modeling. Traditional in vitro models often fall short in replicating human CV physiology, prompting efforts to advance cardiac tissue engineering and computational modeling. Among these, three-dimensional (3D) bioprinting has emerged as a transformative tool, enabling the creation of biomimetic CV constructs that more faithfully replicate native tissue structure and function. However, challenges persist in achieving appropriate mechanical properties and long-term performance of engineered CV constructs. Computational modeling offers powerful solutions to assist with these challenges, providing predictive insights into …

Intravenous Buprenorphine Reversed Opioid-Induced Respiratory Depression Without Precipitating Withdrawal In Emergency Department Patients, Anthony Spadaro, Nicholas Imperato, Jaclyn Scalgione, Cynthia Santos

Faculty, Staff and Student Publications

Objective: Buprenorphine is a high-affinity partial agonist that can displace full opioid agonists and could be used to reverse opioid-induced respiratory depression. There is scant evidence to support the use of buprenorphine in an acute opioid overdose to reverse respiratory depression in the era of high fentanyl prevalence in the drug supply. This is a case series of four patients with signs and symptoms of an opioid overdose who showed clinical improvement after receiving intravenous buprenorphine.

Method: A case series of adult emergency department patients treated at a single urban academic medical center was reviewed by the authors, who determined …

Estrogen As A Therapeutic Agent: Targeting Endotheliopathy And Coagulopathy In Trauma, Niruktha Raghavan, Mira Patel, Bryan A Cotton, Julia Coleman, Donald Jenkins

Faculty, Staff and Student Publications

Background: Traumatic injury remains a leading cause of death, often due to uncontrolled hemorrhage and trauma-induced coagulopathy (TIC). TIC is critically triggered by the endotheliopathy of trauma, involving endothelial cell injury and glycocalyx degradation. Despite conventional advances, the stable casualty rate underscores the urgent need for new precision resuscitation strategies that acknowledge factors like biological sex.

Design: The central hypothesis is that estrogen confers a survival advantage in hormonally active females through a dual action: endothelial cell stabilization (halting glycocalyx shedding) and its inherent procoagulant effect. The analysis assesses the therapeutic potential of both exogenous estrogen and female-derived blood products …

Severe, Non-Apneic Respiratory Dysfunction And Hypoxia Following Generalized Convulsive Seizures, Haley E Pysick, Rup K Sainju, Roshni Nair, Deidre N Dragon, Eduardo Bravo, Laura Vilella, Xiaojin Li, Samden D Lhatoo, George B Richerson, Brian K Gehlbach

Faculty, Staff and Student Publications

Objective: Sudden unexpected death in epilepsy (SUDEP) is a devastating consequence of some generalized convulsive seizures (GCS). Recent work has focused on seizure related apnea as a biomarker of SUDEP risk, frequently without characterizing the adequacy of non-apneic ventilation or identifying other dysfunctional breathing patterns. We hypothesized that GCS frequently induce immediate, severe, non-apneic respiratory dysfunction that can induce critical hypoxia and bradycardia and sought to characterize breathing patterns after GCS.

Methods: Adult patients admitted to an epilepsy monitoring unit were studied. The effects of GCS on breathing and heart rate were analyzed using nasal pressure transducers, chest and abdominal …

Plasma Constituents Promote Endothelial Thromboinflammatory Dysfunction After Hemorrhagic Shock, Kelly E Sanders, Marissa D Pokharel, Baron K Osborn, Yao-Wei Wang, Charles E Wade, Lucy Z Kornblith, Mitchell J Cohen, Jessica C Cardenas

Faculty, Staff and Student Publications

Background: Hemorrhagic shock (HS) following traumatic injury is hallmarked by innate immune activation, hypercoagulability, and thromboinflammatory complications. To date, the direct link between HS, injury severity, and endothelial cell (EC) contributions to thromboinflammation remains poorly understood. The goals of this study were to determine the impact of injury severity and HS on endothelial-mediated thromboinflammation and examine whether these changes increase the propensity for thrombosis.

Methods: Plasma from 89 male trauma patients, stratified by HS and injury severity, and 10 healthy subjects were assessed for inflammatory mediators by BioPlex. Human lung microvascular endothelial cells were exposed to patient plasma for 4 …

Antidepressant-Like Effects Of Extinction Learning As An Animal Model Of Behavioral Therapy, Jing Liu, Sarah E Bulin, David A Morilak

Faculty, Staff and Student Publications

Exposure-based behavioral therapy, the most effective treatment for posttraumatic stress disorder (PTSD), also reduces depressive symptoms. However, neurobiological mechanisms underlying the beneficial effects of exposure-based behavioral therapy on depression remain unknown. Our lab has established fear extinction as a rat model of exposure therapy to investigate the mechanisms underlying its therapeutic behavioral effects in chronically stressed rats. In this study, we demonstrated that extinction learning reduced immobility in the forced-swim test and reversed chronic stress-induced reduction in sucrose preference. Chemogenetic inactivation of pyramidal neurons in the ventral medial prefrontal cortex (vmPFC) prevented these antidepressant-like effects of extinction. Extinction learning enhanced …

Effects Of Tourist Feeding On The Gut Microbiome And Ecophysiology Of Endangered Northern Bahamian Rock Iguana (Cyclura Cychlura), Erin Lucy Lewis

All Graduate Theses and Dissertations, Fall 2023 to Present

The lifespan and reproduction of an animal depends on maintaining a healthy physiology. The proportion of individuals that survive to an age at which procreation can occur is important to long-term stability of populations and contributes to species survival. Health in an animal is measured in a variety of ways, including through monitoring of the body’s physiological systems, as well as its ability to respond to invading pathogens, and through the different types of bacteria present in the gut. Relationships among those different health metrics have been discovered in recent years and have been found to play important roles in …

Freshwater Mussel Reintroduction And Recolonization Habitat Modeling Using Hydraulic, Sediment And Geomorphic Parameters, Megan Elizabeth Dinicola

All Graduate Theses and Dissertations, Fall 2023 to Present

Freshwater mussels are an important group of aquatic animals that live within the riverbed, but their populations have been drastically reduced by habitat alteration and other anthropogenic activities. In an effort to bolster populations, river managers and biologists are using mussel reintroductions as part of their restoration efforts but there are gaps in our understanding of where to reintroduce mussels within a river to see them create self-sustaining populations. This research helps shrink this gap by better understanding the physical habitat needs and population expansion potential of subadult freshwater mussels, or the size of mussel that hatcheries grow for reintroduction, …

Characterizing Metabolic Flux Of Plant Secondary Metabolites From Diverse Animal-Source Foods To Humans, Muhammad Ahsin

All Graduate Theses and Dissertations, Fall 2023 to Present

There is a popular saying: “You are what you eat.” But does this also mean, “You are what your food eats”? This dissertation explores that question by examining how farming practices shape the nutritional quality of meat—and what, if anything, those differences mean for the people who consume it. While modern livestock production and nutrition research often emphasize protein and fat, this work tests the idea that animals function as biological mediators, linking soil and plant conditions to human diets through the chemical composition of meat and milk. To evaluate this hypothesis, we tracked health-relevant plant compounds— phytonutrients—across the soil–plant–animal–human …

Impact Of Inhibiting Mitochondrial Calcium Uptake On Calcium Homeostasis And Postmortem Metabolism In Porcine Muscle, Fatema Rahaman Mishu

All Graduate Theses and Dissertations, Fall 2023 to Present

After harvesting the animal, pork muscle continues to undergo changes as it turns from muscle into meat. During this early period, the muscle uses up energy and produces acid, which lowers pH and can affect important quality traits such as color and how well the meat holds water. A key driver of these early changes is calcium, a natural mineral inside muscle cells that helps control contraction and energy use. In this study, we tested whether mitochondria (the cell’s “powerhouse”) help control calcium after harvest and, in turn, influence early postmortem changes in pork. We used a research compound (DS16570511) …

Influence Of Predation Risk On Elk Feeding Behavior In A Multi-Predator Environment, Rosemarie C. Pugh

All Graduate Theses and Dissertations, Fall 2023 to Present

Sometimes predators don’t just affect prey by killing them, they can also change how prey behave. For example, animals may spend more time watching for danger and less time eating, which could affect their health and survival. In Yellowstone National Park, past research has suggested that elk may reduce feeding because they are trying to avoid predators like wolves and cougars. However, most previous studies have only looked at elk behavior during the daytime and have often looked at responses from single predators rather than multiple.

In this study, I used high-resolution tracking collars equipped with motion sensors to directly …

Effects Of Mineral Supplementation On Ovine Performance, Forage Selectivity, And Rangeland Utilization In Southern Utah, Ryan A. Spurling

All Graduate Theses and Dissertations, Fall 2023 to Present

Sheep producers in the Intermountain West region of the US rely on productive rangeland ecosystems that vary widely in forage composition and nutrient availability. As such, supplementation is often necessary to offset dietary deficiencies. Soils and forages grown on rangelands are known to be highly variable in mineral content. Even so, a third to half of the producers in the Intermountain West choose not to provide a complete mineral supplement. Rangeland environments are often characterized by rugged geographical features that make optimal supplement placement difficult. New advances in technology have allowed us to measure individual animal intake on rangelands via …

Honorata Memoria Prosequar: The Lives Of Republican And Augustan Women As Antecedents For Later Deifications, Megan Fairbanks

All Graduate Theses and Dissertations, Fall 2023 to Present

Emperors and some notable societal figures in the early Roman Empire are known to have practiced apotheosis, a state-sponsored process which confirmed that deceased men, women, and sometimes children had achieved the status of godhood. This process became official during the lifetime of Rome’s first emperor, Augustus, who deified Julius Caesar. As the process developed, it came to involve several formalized elements, including senatorial voting, a public ceremony (consecratio), and the production of commemorative images published through various media such as coins, paintings, and honorific statues. The purpose of this thesis is to determine the possibility of existing precursors for …

How Novices Write Code: Discovering Best Practices, Matt Rau

All Graduate Theses and Dissertations, Fall 2023 to Present

Learning to program is a difficult endeavor, leading to chronically high failure rates in introductory programming courses. One thing that makes teaching programming difficult is that we don’t fully understand what problem solving habits and writing strategies separate successful programmers from struggling ones. Knowing how to teach these habits to a new programmer is an equally difficult challenge,

Studying the way people write code has proved difficult. Until recently, there was very little relevant publicly available data, and no good ways to analyze student programming behavior at a large scale. In this thesis, I address both issues. I publish a …

Effect Of Agroecological Feeding Systems Of Dairy Cattle On The Nutritional Composition Of Milk, Sree Bhavya Immadi

All Graduate Theses and Dissertations, Fall 2023 to Present

How food is produced can influence both its nutritional quality and its impact on the environment. In recent years, regenerative (agroecological) farming practices have gained attention for their potential to improve soil health, biodiversity, and the nutritional value of foods. In dairy production, these practices often involve growing more diverse forage crops, reducing soil disturbance, and relying less on monoculture-grown feed rations. However, there is still limited real-world evidence showing how these practices affect the nutritional composition of milk produced on commercial dairy farms.

This dissertation explores how regenerative feeding and feed production practices influence the nutritional quality of milk, …

Effects Of Cannabinoid Agonist Win 55-212-2 On Latent Inhibition, Ashley Peterson

All Graduate Theses and Dissertations, Fall 2023 to Present

The endocannabinoid system (ECS) is a modulatory system that helps regulate brain functions, including learning and memory, influencing processes such as latent inhibition (LI). LI refers to the slower association formed when a familiar event is paired with a new outcome, compared to the association of an unfamiliar event. LI is a process that is often disrupted in individuals with schizophrenia and is known as a cognitive symptom. This study aimed to determine whether the drug WIN-55-212-2 (WIN), which interacts with the ECS, influences the process of LI using a fear-conditioned response procedure. The results found no significant interaction among …

A High-Throughput Assay For Monitoring And Quantifying Amyloid-Β Accumulation And Clearance In Alzheimer's Disease Cell Models, Ajish Ariyath, Fraulein Denise Arigo, Anna Fyfe, W. M.A.D.Binosha Fernando, Ralph Martins, Prashant Bharadwaj

Research outputs 2022 to 2026

Amyloidogenic proteins, such as amyloid-β (Aβ), self-assemble into cross-β fibrils whose accumulation is central to Alzheimer's disease (AD). Measuring Aβ aggregation and clearance in living cells remains challenging using current cell-based assays, which are often low-throughput or not suited for real-time monitoring. This study aimed to (1) develop a robust, quantitative, and scalable fluorescence-based assay using Amytracker to monitor Aβ accumulation and clearance in an Aβ-producing neuronal cell model, and (2) validate its utility for mechanistic studies and therapeutic screening. We established a plate-based fluorescence assay using Amytracker in MC65 neuronal AD model expressing the Amyloid precursor protein C-terminal fragment …

Diacylglycerol Kinase Eta As A Novel Target In Nras Mutant Melanoma, Haley P. Wilson, Casey D. Stefanski, Signe Caksa, Glenn L. Mersky, Scott D. Varney, Jelan I. Haj, Dan A. Erkes, Timothy J. Purwin, Vivian Chua, Andrew E. Aplin

Research outputs 2022 to 2026

NRAS mutations occur in 10%–30% of cutaneous melanomas and are associated with high tumor mutational burden. Mutant NRAS signaling drives aberrant cell growth and proliferation, in part, through activation of the RAF–MEK-ERK1/2 kinase pathway; however, targeted therapies to this pathway have limited effectiveness in patients with NRAS mutant melanoma. The role of other targetable signaling pathways in NRAS mutant melanoma is poorly characterized. Here, we demonstrated that one isoform of diacylglycerol kinase, diacylglycerol kinase eta (DGKη), a lipid signaling regulator, was highly expressed in NRAS mutant melanoma patient samples. Knockdown of DGKH in NRAS mutant melanoma cell lines resulted in …

Neuromuscular Performance Changes In Response To The Australian Special Forces Selection Course, Angela Uphill, Kristina L. Kendall, Stuart Guppy, Michael Vacher, Simon Parker, Hannah Brown, Brendan Ashmore, Travis Zomer, G. Gregory Haff

Research outputs 2022 to 2026

Introduction: Strenuous military activities, such as Special Forces (SF) selection courses, are known to involve high physical loads in the presence of food and sleep deprivation, which can have detrimental impacts on physical performance. Understanding the magnitude of performance deficit and the time required for recovery associated with these events, is important for injury prevention and maintaining operational readiness. The purpose of this study was to investigate the changes in physical performance in response to the Australian SF Selection Course (SF-SC) and to monitor recovery throughout 8 weeks following the course. Materials and Methods: Ninety-three healthy male participants (age: 28 …

The Secret Science Of Songs. Created By All Children Everywhere, Paul G. Morehouse Phd

The Transdisciplinary STEAM+ Journal

This field article introduces The Secret Science of Songs, a research-informed, practice-based framework that reconceptualizes songs as powerful, transdisciplinary tools in early childhood education. Drawing on over 40 years of classroom-based action research alongside established findings in cognitive science and music perception, the framework argues that young children’s engagement with songs is rooted not primarily in performance skill, but in their innate capacity to perceive and process auditory patterns in music and language. These pattern-based experiences constitute a rich aesthetic and cognitive domain through which children construct meaning, long before mastery of melody or verbal precision emerges.

The article …

Fractsynth: Exploring Glitch Timbres With Chaos Theory, Aidan Roach

The Transdisciplinary STEAM+ Journal

In this paper, I explore how chaos theory can be used to design a new kind of synthesizer with the primary focus of producing glitchy, unpredictable sounds. Glitch music embraces abstract sound design, malfunctioning electronics, and randomness as the main compositional elements. However, most synthesizers rely on stable, repetitive oscillators that often sound too controlled. To challenge this, I developed FractSynth, a real-time synthesizer that uses chaotic attractors–including the Logistic Map, Henon Map, and Lorenz System–as modulation sources for frequency, amplitude, and tone. The software also features real-time Lyapunov Exponent Tracking, which gives users a direct visual of how …