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Rethinking Vaginal Microbiome Resilience: A Conceptual Multi-Omic Framework, Brittnee Cagle-White, Rob E. Carpenter, Alaina Vincent, Ellen Kominek, Andrew Krouse

Human Resource Development Faculty Publications and Presentations

The vaginal microbiome is often interpreted through static taxonomic patterns. Yet microbial composition alone does not explain why some communities resist perturbation, recover after disruption, or transition toward dysbiosis. This narrative review synthesizes evidence that vaginal microbiome stability is shaped by endocrine phase, epithelial substrate availability, microbial functional capacity, mucosal tone and candidate host modifiers. High-estrogen states, particularly pregnancy, are associated with epithelial maturation, glycogen accumulation, low vaginal pH, and Lactobacillus-dominant communities, whereas postpartum, lactational, menopausal, and other hypoestrogenic states are associated with reduced epithelial support and increased vulnerability to diverse anaerobe-rich configurations. We review the linking of the estrogen–glycogen–Lactobacillus …

An Anthropological Lens Into Human Milk Mirnas: Roles In Evolution And Epigenetic Programming Of Infant Health, Samantha Wu, Erika Yu, Allison Oh, Chantal Rabay, Amy L. Non

Human Biology Open Access Pre-Prints

MicroRNAs (miRNAs) represent an understudied bioactive component of human milk with an important epigenetic role in regulating infant gene expression. Most anthropological studies of human milk have focused on the evolutionary and biocultural significance of milk macronutrient content for infant development. This review will focus specifically on the value of studying milk miRNAs, a critical bioactive component of milk that may transmit cues to offspring about maternal environments. These cues could epigenetically program infant development and shape health trajectories throughout the life course. MiRNAs are short, single stranded, ribonucleic acids that serve as post-transcriptional regulators of gene expression which can …

Combining Menin And Mek Inhibition To Target Poor Prognosis Kmt2a-Rearranged Ras Pathway-Mutant Acute Myeloid Leukemia., Nastassja Scheidegger, Constanze Schneider, Gabriela Alexe, Yi-Cheng Wang, Todd A. Alonzo, Allen Basanthakumar, Wallace A. Bourgeois, Julia Dudkiewicz-Garbicz, Delan Khalid, Lucy A. Merickel, Jennifer A. Perry, Rhonda E. Ries, Silvi Salhotra, Audrey Taillon, Alan S. Gamis, Richard Aplenc, Marian H. Harris, Mark Wunderlich, Scott A. Armstrong, Jessica A. Pollard, Soheil Meshinchi, Yana Pikman, Kimberly Stegmaier

Manuscripts, Articles, Book Chapters and Other Papers

KMT2A-rearranged (KMT2A-r) acute leukemias are especially prevalent in the pediatric population. KMT2A-fusion proteins drive leukemogenic gene expression through an interaction with a chromatin complex that includes the scaffold protein menin, giving rise to aggressive acute leukemias. RAS pathway mutations are also common in pediatric leukemia. In a cohort of 1750 patients enrolled on Children's Oncology Group (COG) trials, we identified RAS pathway mutations in 43% of acute myeloid leukemia (AML) cases. The presence of RAS pathway mutations in KMT2A-r AML was associated with a lower complete remission rate, poor event-free survival and overall survival (OS), and early relapses. Given the …

Influence Of A New Nanoensemble Of Graphene Oxide And Indole-3-Acetic Acid On The Morphophysiological, Biochemical, And Ultrastructural Characteristics Of Upland Cotton (Gossypium Hirsutum L.), Nurlan Amrahov, Ulviyya Hasanova, Aliyeva Gunel, Aghazada Goncha, Fuad Rzayev, Eldar Gasimov, Aliyeva Solmaz, Lala Gahramanli, Elvin Ibrahimli, Ruhangiz Mammadova, Zarema Gakhramanova, Cem Bayram, Goncha Eyvazova, Si̇bel Hasanova, Ziyaddin Mammadov

Turkish Journal of Botany

​The exogenous application of phytohormones plays a crucial role in the activation of adaptive mechanisms in plants under both normal and stress conditions. Therefore, the present study investigates the synthesis, characterization, and application of nanoensembles based on indole-3-acetic acid (IAA) and graphene oxide (GO) nanolayers. The surface of GO synthesized by the modified Hummer method was noncovalently functionalized with IAA molecules. The structure and morphological properties of the prepared materials were analyzed by Fourier transform infrared spectroscopy, X-ray diffraction, UV-Vis spectroscopy, and transmission electron microscopy. The IAA and IAA+GO nanoensemble enhanced the morphological parameters, lateral root formation, catalase and peroxidase …

Effects Of Calcium And Magnesium Ions On Wheat (Triticum Aestivum) Chromosome Structure Visualized Using Light And Scanning Elec​Tron Microscopes, Andi Salamah, Fitri Amalia Nurchasanah, Vycka Fikriyani Fairuz, Astari Dwiranti

Turkish Journal of Botany

Divalent cations, particularly calcium (Ca2+) and magnesium (Mg2+), are essential for maintaining chromosomal architecture, yet their roles in plants remain poorly characterized. This study investigated the effects of Ca2+ and Mg2+ on the chromosomal structure of wheat (Triticum aestivum, 2n = 6x = 42), a widely used model in plant cytogenetics. Root-tip chromosomes were isolated enzymatically and treated with BAPTA (a Ca2+-specific chelator), EDTA (a broad cation chelator), and buffer solutions containing either 5 mM Mg2+ (XBE5) or no Mg2+ (XBE). Chromosomes were stained with aceto-orcein for light microscopy and processed for scanning electron microscopy (SEM). Microscopic observations revealed that …

Systematic Implications Of Seed Morphology In Oxytropis (Galegeae: Fabaceae) From Pakistan, Amjad Khan, Amir Sultan, Raees Khan, Rozina Rozina, Shahab Ali, Ali Bagheri, Rifat Ullah Khan, Abdul Samad Mumtaz, Tariq Ullah, Ghalia Saleem Aljeddani, Hasil Khan

Turkish Journal of Botany

Oxytropis is a taxonomically challenging genus within the tribe Galegeae of the family Fabaceae. Despite its systematic importance, the seed morphology of Oxytropis has been poorly investigated. In the present study, we examined the seed morphology of 18 Oxytropis species representing 10 sections from the Pakistan, with the aim of evaluating the diagnostic value of seed traits using both light and scanning electron microscopy. The current investigation revealed two distinct seed shapes, namely cordiform and prolonged reniform, across the studied taxa. Seed length ranged from 1.75 mm in O. crassiuscula to 2.3 mm in O. duthieana, while seed width varied …

Optimized Immunoprecipitation Identifies Candidate Proteins Associated With Eyespot Protein Eye2 In Chlamydomonas Reinhardtii ​, Esra Öztürk, Telsa Mittelmeier, Carol Dieckmann, Atakan Sukatar

Turkish Journal of Botany

​The EYE2 protein is essential for eyespot assembly and localization in Chlamydomonas reinhardtii, yet the molecular mechanisms underlying its function remain poorly understood. In this study, we present a systematic evaluation and optimization of an immunoprecipitation (IP)-based workflow for isolating EYE2 and its associated protein complexes. Key methodological variables including strain selection, flagella removal, and detergent concentration were compared using western blotting to determine optimal extraction conditions. The combination of the cell wall-deficient cw15 strain, 4% digitonin extraction, and pH shock-mediated deflagellation significantly enhanced IP specificity and reproducibility. Using this optimized protocol, EYE2 and its potential interaction partners were isolated …

Different Chitinase Activity In Nutrient-Enriched Multi-Antagonistic Streptomyces Sp. And Trichoderma Sp. Against Root-Knot Nematodes Meloidogyne Sp., Tri Mujoko, Penta Suryaminarsih, Safira Rizka Lestari

Baghdad Science Journal

The threat of root-knot nematodes to horticultural and plantation crops is a grave concern. To combat this issue, using bacteria and fungi as biological control agents is an effective and efficient approach. This research aims to evaluate the efficacy of Streptomyces sp. strains PS23104 and Trichoderma sp. when grown on nutrient-enriched media as a biological control agent for root-knot nematodes Streptomyces sp. PS23104 and Trichoderma sp. were cultured on enriched EKG media with malt extract, glucose nitrate, pure coconut water, and molasses, while potato extract media without nutrient addition served as the control. The findings indicate that both Streptomyces sp. …

Bioactivity Of Chitosan Coated Nanoparticles Biosynthesized Using Yeast Against Hypervirulent Klebsiella Pneumoniae (Hvkp), Sura Alaa Suod, Huda Suhail Abid Al-Hayanni

Baghdad Science Journal

Multidrug-resistant (MDR) bacteria are becoming one of the most pressing problems worldwide and are becoming difficult to treat; therefore, nanotechnology is a promising alternative technology. The current study aimed to biosynthesize chitosan Saccharomyces cerevisiae nanoparticles (ScNPs) and evaluate their antibacterial activity against hypervirulent Klebsiella pneumoniae (HvKp). Five K. pneumoniae isolates from different clinical sources were screened for extended-spectrum beta-lactamase (ESBL) production and virulence tests. ScNPs were synthesized and characterized. The antibacterial activity of ScE and ScNPs was determined. The result showed that five bacterial isolates were confirmed as K. pneumoniae and displayed highly virulent and resistant capabilities, hence classifying them …

Association Of Calcium-Sensing Receptor (Casr) Gene Polymorphisms With Calcium Stone Disease In Babylon Province, Iraq., Hadi Sajid Abdulabbas, Yasir Haider Al-Mawlah, Aamal Muhsen Kadhum

Baghdad Science Journal

In an effort to identify the polymorphism's ultimate function in calcium stone development, an evaluation of the link between calcium stone disease and CaSR polymorphism in the population of Babylon Province, Iraq, was performed. 60 patients were hospitalized at Hillah Hospital in the province of Babylon. Blood samples were collected between January and December 2020. Sixty patients diagnosed with renal stones and forty healthy controls were included in the study. CaSR single-nucleotide polymorphisms (SNPs) were genotyped using the single-strand conformation polymorphism technique after a polymerase chain reaction. In order to confirm these DNA polymorphisms, DNA sequencing was employed. The findings …

Prevalence Of Torovirus, Rotavirus, And Adenovirus Infection Among Iraqi Pediatric Patients With Gastroenteritis, Eman A. Mahmood, Nadira S. Mohamed, Safaa Al-Deen A. S. Al-Qaysi

Baghdad Science Journal

Gastroenteritis is a major cause of morbidity and mortality among children, particularly in developing countries. Rotavirus, adenovirus, and torovirus are among the common viral agents associated with pediatric gastroenteritis. This study aimed to determine the prevalence of these viruses and to evaluate diagnostic methods for torovirus infection among Iraqi children. A total of 250 stool samples were collected from pediatric patients aged from 2 weeks to over 10 years who attended Children's Protection Teaching Hospital, Baghdad, Iraq, between December 2023 and August 2024. The samples were screened for the presence of rotavirus, enteric adenovirus, and torovirus antigens using the Hemagglutination …

Longitudinal Patterns In Stygobiont Distribution In The Hyporheic Zone Of The Karst Spring-Fed San Marcos River, Texas, Usa, Kenneth Sparks, Benjamin F. Schwartz, Safra Altman, Kathryn E. Perez, Zoey B. Chanin, Caroline Mierzejewski, Benjamin T. Hutchins

School of Integrative Biological & Chemical Sciences Faculty Publications

Groundwater-obligate taxa (stygobionts) exhibit variable degrees of habitat specialization, ranging from those restricted to single micro-habitats to generalists occurring in a broad range of habitat types. The karstic Edwards Aquifer is the most biodiverse and well-studied groundwater habitat in the continental United States, but some Edwards Aquifer-associated species also occupy the hyporheic zone of rivers downstream from karst. The goals of this research were to 1) assess the potential of the off-karst hyporheic zone as habitat for ‘karst’ species and 2) to determine how stygobiont richness, diversity, and abundance related to distance from karst in the hyporheic zone of a …

Distributed Impressions: How Multiple Minds Come Together To Shape Person Perception, Lindsey J. Tepfer

Dartmouth College Ph.D Dissertations

Moments after perceiving another person, we immediately infer a wealth of information about them. Despite the complexities within our social environments, we distill an incredible magnitude of details available to us into compressed representations of other people’s minds and mannerisms. Yet research has focused largely on mapping isolated stimuli—divorced from its messy context—to the spontaneous impressions they provoke. As a result, there are many unanswered questions about how our judgments are influenced by the rich and noisy social world we inhabit. Here, I’ve leveraged two paradigms to investigate how impression formation and updating may operate when the constraints of the …

Bridging Pediatric To Adult Care In Cystinosis: A Scoping Review Of Healthcare Transition Of Care Protocols And A Roadmap For Future Practice., Sanat Subhash, Nicholas Pantelakis, Kyle Backston, Ruhaan Bajpai, Amir Safadi, Brandon Petrovich, Bradley A. Warady, Maria Diaz-Gonzalez De Ferris, Rupesh Raina

Manuscripts, Articles, Book Chapters and Other Papers

Cystinosis is a rare lysosomal storage disorder that affects approximately 1 in 100,000 to 200,000 live births worldwide. Long-term graft success in cystinosis depends on cysteamine adherence and structured multidisciplinary transition, supported by protocols developed by both pediatric nephrologists and hospital organizations. This review explores institutional and regional care models, structured programs such as the RISE protocol and Got Transition's Six Core Elements, and major barriers including adult nephrology expertise, psychosocial stressors, and insurance coverage gaps. Additionally, we explore the impact of digital health tools for their role in supporting cysteamine adherence and maintaining continuity of care. Emerging technologies such …

Evaluation Of Cytotoxic Effect From Clinacanthus Nutans Leaf Extract On Mda-Mb231 Cells Expressing Cd44+/Cd24–, Siti Syarifah, Tri Widyawati, Sri Melinda Kaban, Nor Adlin Md Yusoff

Baghdad Science Journal

Breast cancer prevalence and incidence are increasing globally every year. Medication resistance and recurrence are more likely in breast cancer cells that express CD44⁺/CD24^–. C.nutans leaves are a traditional medicinal herb with numerous pharmacological benefits, including antioxidant and antiproliferation properties, although the activity against breast cancer stem cells is still unclear. Method: In this study, C.nutans leaves were extracted using ethanol solvent with graded concentrations of 100%, 80%, 60%, 40%, 20%, and 0% (water), respectively, by maceration. We used isolated MDA-MB231 cells with high expression of CD44⁺/CD24^– by using magnetic-activated cell sorting …

Study Of The Correlation Between Asprosin And Insulin Resistance In Obese And Normal-Weight Pcos Patients, Walaa Najm Abdulwahid, Reem M. Obaid

Baghdad Science Journal

Fatty tissue is considered an endocrine organ that produces a type of hormone called Adipokine; this hormone is specifically secreted by adipose tissues, such as asprosin. Polycystic Ovarian Syndrome (PCOS) is the most common hormonal condition throughout the years of reproduction in females, manifesting with a significant frequency of decreased glucose tolerance, increased serum androgen levels, and type 2 diabetes among female sufferers who are overweight or obese. The current investigation included 120 PCOS patients (40 obese, 40 normal weight) and 40 healthy women; samples were collected from July to December 2024 at Kamal al-Samarrai Hospital in Baghdad, Iraq. Serum …

Histopathological And Immunological Effects Of Tamanu Oil Nanoemulsion On Imiquimod Induced Psoriasis In Albino Rat Skin, Israa Mahmood Mohammed, Treefa Farouq Ismail

Baghdad Science Journal

Psoriasis is an autoimmune disease caused by T-cell activation. This condition is characterized by skin proliferation and inflammation. This study aimed to enhance tamanu oil bioavailability by green synthesis method, in order to evaluate the potential anti-psoriatic benefits of tamanu oil nanoemulsion in rat skin, based on observational, histopathological, and immunological outcomes. The formulation was prepared by phase inversion temperature technique. The physical and chemical characteristics include: droplet morphology, size, zeta potential, pH, and stability. The formulation exhibited a spherical and small globule size of less than 100 nm, good zeta potential (–23.9 mV), suitable pH (5.3) and physically stable. …

Cytokine Profiles In Rheumatoid Arthritis: Relationship Of Tnf-Α , Il-17, And Il-13 With Autoantibodies (Rf And Anti-Ccp) And Clinical Disease Severity, Sonia Elia Ishaq, Taban Kamal Rasheed, Niaz Albarzingi

Baghdad Science Journal

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and autoantibody production. This study aimed to assess serum levels of TNF-α , IL-17, and IL-13 in RA patients and to explore their association with disease severity and autoantibody status in comparison to healthy controls. The study involved 67 RA patients and 20 healthy individuals. Patients were initially classified based on autoantibody status (RF-positive vs. RF-negative and anti-CCP-positive vs. anti-CCP-negative), and further categorized into low, moderate, and high disease activity groups. Cytokine levels were measured by ELISA, CRP and RF by turbidimetry, and anti-CCP levels by electrochemiluminescence immunoassay. …

Foliar Application Of Methyl Jasmonate And Titanium Nanoparticles Mitigates The Negative Effects Of Salinity On Lettuce Plants, Tahani J. Hijazin

Baghdad Science Journal

Salinity stress is one of the major abiotic factors that significantly impact plant growth. In this regard, this work investigated a way to alleviate the adverse impacts by simultaneously spraying methyl jasmonate and titanium nanoparticles on lettuce (Lactuca sativa L.) plants, which are grown under induced salinity conditions. Two concentrations of salt (100 and 150 mM NaCl) were applied to the plants. After that, simultaneous spraying of methyl jasmonate (0.5 and 1 mM) and titanium nanoparticles (100 and 200 mg/L) was performed on lettuce leaves. Following the application of the previous treatments, the plants were collected, and various …

Effects Of Modified Cryoprotectants On The Expression Of Heat Shock Protein 90 (Hsp90) And Zona Pellucida Ii (Zpii) Of Post-Thawing Sperm Quality, Silvia Werdhy Lestari, Manggiasih Dwiayu Larasati, Gita Pratama, Mulyoto Pangestu, Muhammad Thoriq, Dantha Rafael Sitompul

Baghdad Science Journal

Sperm cryopreservation is a basic element of assisted reproductive technology, yet it often compromises cell viability through thermal and osmotic stress. The cellular response involves HSP90, a stress marker and ZPII, which is essential for sperm-oocyte interaction and preventing polyspermy. In addition, this modified cryoprotectant combines glycerol and raffinose is expected to reduce damage and increase spermatozoa's recovery rate after cryopreservation. To address these challenges, we investigated the expression levels of HSP90 and ZPII to assess the protective capacity of modified cryoprotectant formulations. These parameters were analyzed pre- and post-cryopreservation to establish whether these novel mediums superiorly preserve sperm integrity …

What Are Vestigial Structures? Evidence For Evolution Explained, Ryan Bebej

University Faculty Publications and Creative Works

Evolution wasn’t something we talked about very much in my home or church when I was growing up.

Yet somehow, I came to believe that evolution wasn’t compatible with Christian faith. At the time that seemed okay, because I had heard that the evidence for evolution wasn’t very strong anyway.

That all began to change when I started studying biology in college. In different classes, I encountered some of the compelling evidence for evolution and common descent.

Vestigial structures were one of the topics that came up often in biology textbooks about these topics.

Today, biologists point to …

The Exploration Of The Antidiabetic Potentialities Of Bottle Gourd (Lagenaria Siceraria) Fruits Through In Vivo, In Vitro And In Silico Approaches, Arifa Sultana, Fahima Aktar, Fabiha Noushin, Mariam Wahed, Jakir Ahmed Chowdhury, Abu Asad Chowdhury, Shaila Kabir, Md. Shah Amran

Pharmaceutical Sciences and Research

Due to the ineffectiveness, side effects, and high cost of conventional drugs, medicinal plants grabbed the attention to treat diabetes, which has been a global health concern nowadays. Therefore, this study aims to investigate the antidiabetic potentialities of bottle gourd (Lagenaria siceraria) fruits in in vivo, in vitro, and in silico methods. For the in vivo assessment, alloxan-induced diabetic rats were fed food mixed with bottle gourd powder for 21 days. For the in vitro assessment, the alpha-amylase inhibitory assay was used to determine the antidiabetic properties of an ethanolic extract of L. siceraria. For the …

Hold-Then-Release Safety-Gated Streaming With Dual-Lane Classification And Abuse-Sample Harvesting For Anonymous Public Llm Surfaces, Gustavo Matthew Assuncao

Defensive Publications Series

This defensive publication discloses a runtime architecture for anonymous, unauthenticated public large-language-model (LLM) surfaces — the "ask a question" box on a marketing website, a public demo endpoint, an embedded help widget — that simultaneously preserves token-by-token streaming user experience and enforces an absolute zero-leak output-safety guarantee. The core mechanism is hold-then-release: the final model answer is streamed from the inference backend into an in-process delta buffer and is never forwarded to the client during generation; output safety executes against the fully assembled answer text; only a clean (or explicitly flagged-but-tolerated) verdict causes the buffered deltas to be replayed to …

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Tails From The Isle Of Devils: Two New Records Of Non-Native Invasive Scorpions (Arachnida: Scorpiones) Successfully Intercepted In Bermuda, Including The First Record Of Caraboctonidae, Danniella Sherwood, Mark Outerbridge, Struan R. Smith, Jenny Gosling, Myles Darrell

Euscorpius

Hadruroides sp. (Scorpiones: Caraboctonidae) is newly recorded as a successfully intercepted non-native invasive species in Bermuda, based on examination of a desiccated and damaged immature female specimen. A second record of an intercepted Centruroides gracilis (Latreille, 1804) (Scorpiones: Buthidae) is reported based on examination of an adult male.

An Analysis Of The Law On The Prohibition Of Fraud In Goods, Samira Krourou

Revue Marocaine de Droit, d'Economie et de Gestion (Moroccan Journal of Law, Economics and Management)

One of the most important aspects of consumer protection is the fight against fraud in goods—a form of protection enshrined in the Abrahamic religions long before the enactment of positive laws or the emergence of consumer advocacy movements that spread from the United States to Europe and the rest of the world. While fraud in general is indicative of a malicious tendency to infringe upon rights, spread corruption, and undermine interests, fraud involving goods and food is an even more heinous and dangerous crime because it affects the health and lives of both humans and animals. Therefore, it was necessary …

Vestirse Con Un Cuadro”: El Mantón De Manila En La Encrucijada Entre Tradición Y Modernidad En Fortunata Y Jacinta De Benito Pérez Galdós, Ines Corujo Martin

Publications and Research

Este artículo analiza la relevancia sociocultural que adquirió el mantón de Manila en la España de finales del siglo XIX, tomandocomo punto de partida las diversas representaciones de esta prenda en la novela Fortunata y Jacinta(1887) de Benito Pérez Galdós. Como se propone, el mantón, prenda de origen asiático cuyo surgimiento está ligado a la ruta comercial colonial de los Galeones de Manila, constituye en el texto galdosiano un objeto clave para explorar algunos de los debates centrales del imaginario cultural finisecular en torno a la identidad nacional, forjada entre el tradicionalismo y la modernidad. El llamativo accesorio, ligado a …

Effect Of Extraction Methods On Emulsifying Properties Of Corn Fiber Gum, Wu Chenglong, Ren Ying, Li Xingguo, Tang Leiming, Sun Xiaohong

Food and Machinery

[Objective] To investigate the influence of three extraction methods (alkaline, enzymatic, and acidic) on the emulsification characteristics of corn fiber gum (CFG). [Methods] Corn bran was used as the raw material, and CFG was extracted using sodium hydroxide, xylanase, and formic–acetic acid–water solutions. The changes in particle size, zeta potential, rheological properties, creaming index, appearance, and microstructure of CFG emulsions were measured. [Results] All three CFG emulsions exhibited shear-thinning behavior, with viscosities decreasing as the shear rate increased. The enzymatically extracted CFG emulsion had the highest viscosity, best gel properties, smallest particle size [(751.6 ± 22.5) nm], highest absolute zeta …

Strategy For Linear Range Broadening Of Elisa Based On Antibody Synergistic Combination, Guo Cuili, Peng Juan, Lai Weihua, Xing Keyu, Gan Bei

Food and Machinery

[Objective] To broaden the linear range of ELISA for deoxynivalenol detection. [Methods] This paper obtains three antibodies with different sensitivities via mouse immunization, cell fusion and screening, ascites preparation, and antibody purification. By optimizing the working concentration and composition ratio of the antibody synergistic combination, the optimal antibody synergistic combination ratio is applied to ELISA to determine the linear range. [Results] The optimal reaction conditions for the ic-ELISA based on synergistic antibodies are as follows: the optimal working concentrations of the coating antigen and antibody are 0.3 and 0.1 μg/mL, respectively. The linear range of the synergistic antibody is obviously …

Ameliorative Effects And Mechanisms Of Crocetin On Insulin Resistance In High-Fat Diet-Induced Diabetic Mice, Wen Jing, Qin Si, Lyu Chenghao

Food and Machinery

[Objective] To investigate the hypoglycemic, lipid-lowering, anti-inflammatory, and antioxidant effects of crocetin in high-fat diet (HFD)-induced diabetic mice, and to preliminarily explore its regulatory effects on hepatic insulin signaling pathways and protein S-nitrosylation levels. [Methods] An insulin resistance model was established in C57BL/6J mice by HFD feeding. Mice were administered low (5 mg/kg), medium (10 mg/kg), or high (20 mg/kg) doses of crocetin for 8 weeks, with metformin (200 mg/kg) used as a positive control. Fasting blood glucose, oral glucose tolerance, serum insulin, lipid profiles, hepatic oxidative stress, and inflammatory cytokines were measured. Western blot was used to detect phosphorylation …

Study On Structural Characteristics And Immunomodulatory Activity Of Flammulina Velutipes Crude Polysaccharide, Wang Xinnuo, Peng Chujun, Jin Yumeng, Zhang Molan, You Lijun

Food and Machinery

[Objective] To prepare Flammulina velutipes crude polysaccharide (FVP) and evaluate its structural characteristics and immunomodulatory activity. [Methods] FVP was prepared from Flammulina velutipes using hot-water extraction and ethanol precipitation, and its structural characteristics were characterized by combining Fourier transform infrared spectroscopy (FTIR) and high-performance size-exclusion chromatography–multi-angle laser light scattering–refractive index detection (HPSEC–MALLS–RI). The immunomodulatory activity of FVP was evaluated using RAW 264.7 macrophages and a cyclophosphamide-induced immunosuppressed mouse model. [Results] The total sugar content of FVP is (51.39 ± 1.36) g/100 g. Structural characterization results show that FVP has characteristic features of a β-type pyranose ring and exhibits a multi-component …