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A Catecholamine-Independent Pathway Controlling Adaptive Adipocyte Lipolysis, Xiao Zhang, Sreejith S Panicker, Jordan M Bollinger, Anurag Majumdar, Rami Kheireddine, Lila F Dabill, Clara Kim, Brian Kleiboeker, Fengrui Zhang, Kristann L Magee, Adam Kepecs, Gretchen A Meyer, Irfan J Lodhi, Erica L Scheller, Et Al.

2020-Current year OA Pubs

Several adipose depots, including constitutive bone marrow adipose tissue, resist conventional lipolytic cues. However, under starvation, wasting or cachexia, the body eventually catabolizes stable adipocytes through unknown mechanisms. Here we developed a mouse model of brain-evoked depletion of all fat, including stable constitutive bone marrow adipose tissue, independent of food intake, to study this phenomenon. Genetic, surgical and chemical approaches demonstrated that catabolism of stable adipocytes required adipose triglyceride lipase-dependent lipolysis but was independent of local nerves, the sympathetic nervous system and catecholamines. Instead, concurrent hypoglycaemia and hypoinsulinaemia activated a potent catabolic state by suppressing lipid storage and increasing catecholamine-independent …

Mouse Alport Podocytes Are Susceptible To Aav9 Transduction In Vivo, Meei-Hua Lin, Kohei Omachi, Joshua F Begin, Jennifer L Richardson, Jeffrey H Miner

2020-Current year OA Pubs

INTRODUCTION: Alport syndrome features a defective glomerular basement membrane (GBM) due to variants in COL4A3, COL4A4, and COL4A5. The most severe forms, which lack the GBM's collagen α3α4α5(IV) network, progress from hematuria in early childhood to proteinuria, chronic kidney disease, and kidney failure by the age of ∼30. As a monogenic disease without specific treatments, and with podocytes being the only glomerular cells that synthesize collagen α3α4α5(IV), the ability to efficiently deliver genes to Alport podocytes could open up new possibilities for treatment.

METHODS: As a proof-of-concept study, we investigated whether podocytes in X-linked Alport mice were susceptible to transduction …

Investigating The Impact Of Carboxylated Polystyrene Nanoplastics In The Liver Using Cell Lines And Precision-Cut Liver Slices, Namrata Pandey, Paula Boeira, Nathaniel J. Clark, Eleanor T. Walker, Somaiah Aroori, Jemimah Denson, Richard C. Thompson, Matthew E. Cramp, Shilpa Chokshi, Ashwin Dhanda

Peninsula Medical School

Background and Aims: Increasing reports of plastic accumulation in human tissue have raised concerns about potential adverse health outcomes. Evidence of negative effects of nanoplastics is heterogeneous and provides limited insights into the underlying pathogenic toxicity mechanisms in humans. Methods: In the present study, carboxylate-modified fluorescently labelled polystyrene nanoparticles (PS NPs) were used to investigate uptake and cytotoxicity in three different hepatic models of varying complexity, including HepG2 cells, IHH cells, and human precision-cut liver slices (hPCLS). Results: The results show model- and dose-dependent effects on hepatocytes. 74.2% ± 13.4% of the IHH cells showed PS NPs uptake at 0.1 …

Small-Molecule Therapies Targeting Tumor-Associated Macrophages In Glioma: Mechanisms And Potential, Hua Wang, Heng Luo, Haijian Zhong, Amy Ockleton, Feng Jiang, Renhui Yi, Shaochun Yang, Ming Li

Peninsula Medical School

Glioblastoma, a highly prevalent and aggressive primary brain tumor, is marked by poor prognosis due to the blood-brain barrier and chemoresistance, which limit conventional pharmacotherapy. Recent research has pivoted toward targeting the tumor microenvironment, where tumor-associated macrophages (TAMs) drive gliomagenesis and malignant progression through immunosuppressive signaling. Small-molecule agents targeting TAMs have emerged as promising therapeutics, capable of reprogramming macrophage polarization, modulating immune cell dynamics, and suppressing glioma growth. This review explores the mechanistic roles of TAMs in glioma and evaluates how small-molecule compounds exert antitumor effects via TAM modulation. These insights lay a foundation for developing next-generation TAM-targeted therapies, offering …

Others’ Requests May Dampen The Desire To Do Good: The Effect Of Requests On Children's Prosocial Motivation Across Five Cultures., Anneliese Skrobanek, Patricia Kanngiesser, Jahnavi Sunderarajan, Jorge David Mantilla Salgado, Saiwa Sisa Quimbo Yacelga, Shoji Itakura, Marie M. Morita, Masanori Yamaguchi, Nadia Chernyak, Lucy M. Stowe, Joscha Kärtner

School of Psychology

There exists cultural variation in how people react to prosocial obligations, but we still know little about when these differences emerge in development and how they can be explained. We investigated this in a study with 6- to 11-year-old children (N = 686) in five cultural contexts. Children listened to scenarios about spontaneous and requested (within-subjects) helping and sharing. They rated requested scenarios as less desirable and less satisfying, but there was significant cross-cultural variation in the magnitude of this effect, which was mostly stable with age. We also measured children's sense of individual agency and internalization of prosocial norms …

Infant Sensory Gating And A Developmental Cascade To Autistic Traits And Anxiety, Rebecca F Schwarzlose

2020-Current year OA Pubs

Disruptions to the infant sensory environment can have lasting effects on neural response properties and behavior in both humans and animals. Recent work has begun to highlight an additional factor in infant sensory experience: differences in inhibitory signaling and sensory gating. Converging work from human and animal studies has begun to implicate a developmental cascade by which impaired sensory gating during a sensitive period of neonatal neurodevelopment promotes a phenotype of sensory over-responsivity, autistic traits, anxiety, and other psychiatric challenges. In this Review, I propose a model for this developmental cascade and highlight how differences in infant sensory responsivity represent …

Coping With Climate Change: The Role Of Plasticity Within And Across Generations, Caitlin Mcandry

School of Biological and Marine Sciences Theses

Climate change is leading to increased mean temperatures and more frequent and severe temperature anomalies, threatening organisms globally. Phenotypic plasticity, the ability of a given genotype to express different phenotypes in different environments, may offer a mechanism that allows organisms to temporarily persist in a rapidly changing environment, ultimately buying time for genetically based adaptations to evolve. Plasticity has been demonstrated both within and across generations, in response to different environmental stressors. However, our understanding of the interactive effects of plastic responses across levels of biological organisation, and over temporal scales from hours to generations, is still limited. Additionally, we …

Investigating The Determinants Of Fight Dynamics In The Minotaur Beetle Typhaeus Typhoeus, Amy Bromley

School of Biological and Marine Sciences Theses

It is widely accepted that the main determinants of contest outcome are resource value, residency status, and resource holding potential, with each of these playing an important role in determining contest behaviour. Examples of resource holding potential are morphological traits such as body size and weaponry. Animals that possess weaponry can exhibit different morphotypes, such as size difference in horns, which can lead to differences in contest behaviour, with larger morphotypes engaging in head-to-head combat, while smaller morphotypes can avoid combat altogether. One such species that possess weapons in the form of horns and engages in physical combat, is the …

Assessing The Ecological Vulnerability Of Natural Flood Defences And Risks To Agricultural Land Following Seawater Flooding, Cally Barratt

School of Biological and Marine Sciences Theses

Anthropogenic climate change is intensifying extreme weather events, affecting the natural environment by altering ecosystems, and leading to habitat loss. Pressingly, the frequency and severity of storm surge flooding in low-lying coastal regions is projected to increase under future climate scenarios. There is a growing need to understand how these events will impact coastal ecosystems, and the measures that can be taken to mitigate against these effects and protect natural resources. Furthermore, with increased pressures placed on agriculture to meet food demand, protecting arable land resources in coastal regions from the rising threat of flooding is imperative. This thesis explores …

Influence Of Ecological Transitions On The Evolution Of Circadian And Light Detection Genes In Squamates, Isobel Sleep

School of Biological and Marine Sciences Theses

Circadian rhythms are self-sustaining, endogenous processes that align behaviour and physiology with daily environmental cycles. Rhythms are regulated by conserved genes forming transcriptional feedback loops and are entrained by environmental cues (e.g., light). Circadian systems are well studied in model organisms, their molecular evolution in squamates (lizards and snakes) remains less understood. Squamates represent the most species-rich reptile group with extensive ecological and sensory diversity, exhibiting multiple independent transitions across ecological niches, including repeated shifts into low-light environments (e.g., nocturnality, fossoriality), making them ideal for examining circadian and light-associated gene evolution. This study investigates how ecological transitions have influenced the …

Autonomous Navigation Development And On-Ground Validation For Satellite Rendezvous And Proximity Operations, Logan Banker

Masters Theses

Spacecraft rendezvous and docking are critical mission phases for various applications of spaceflight, including active debris removal (ADR) and in-orbit servicing, assembly, and manufacturing (ISAM). While previous missions utilized humans to perform rendezvous and docking, this style of mission greatly increases safety risks and cannot be implemented on a large scale. Autonomous servicing satellites provide a path towards scalable rendezvous and proximity operations (RPO) because these autonomous agents do not require human intervention. This work presents a lightweight convolutional neural network (CNN) for the navigation system of an agent which analyzes monocular images and predicts the target's position and orientation …

Wattage- And Composition-Dependent Ocular Surface Toxicity: An In Vitro Study On E-Cigarette Aerosol Exposure In Human Corneal Epithelial Cells, Tzu-Ling Lin

Masters Theses

As electronic cigarette consumption continues to escalate globally, its impact on non-respiratory mucosal surfaces, particularly the ocular surface, remains largely understudied. This study investigated the toxicological effects of e-cigarette aerosols using a human corneal epithelial cell (HCE-2) model. Our findings reveal that aerosol exposure induces a significant, dose-dependent reduction in cell viability, exacerbated by higher device wattage and specific flavor agents such as watermelon. Exposure was found to trigger intracellular oxidative stress and the activation of apoptotic pathways, leading to programmed cell death. Furthermore, the upregulation of proinflammatory cytokines suggests that vaping may predispose users to chronic inflammatory conditions of …

Aura-Visionpose: Creating Datasets For Vision-Based Pose Estimation, Mohanad Alameer

Masters Theses

"Spacecraft rendezvous is an integral part of many orbital missions, including active space debris removal (ADR) and in-orbit servicing, assembly, and manufacturing (ISAM). Traditionally, rendezvous missions rely mainly on manual ground-based control to perform rendezvous and docking. While this can be effective on an individual basis, it becomes less practical at scale. Autonomous vision-based methods can reduce reliance on human control, and may improve scalability, response time, and reduce the possibility of human error for both ADR and ISAM. This work lays the necessary groundwork for developing a machine learning (ML) model for autonomous rendezvous by creating a labeled image …

Moth Assemblages Of Fort Leonard Wood, Missouri, Kimberly Anne Steese

Masters Theses

"Moths (Lepidoptera) are ecologically important insects that function as herbivores, pollinators, prey resources, and indicators of ecosystem integrity. Despite their ecological significance, moth communities in the Missouri Ozarks remain poorly documented, particularly within military-managed landscapes. This thesis examined patterns of moth diversity, assemblage structure, and environmental drivers at Fort Leonard Wood (FLW), Missouri, while also assessing the distribution and genetic diversity of species within the genus Papaipema.

Field surveys were conducted from 2019 through 2021 across prairie, savanna, forest, and restoration habitats at FLW using UV bucket traps, sheet trapping, and targeted larval surveys. Across all sampling efforts, 29,116 individual …

General Approach To Determining The Embodied Energy Of Refractory Products, Jacob A. Kerr

Masters Theses

"Refractories are an important group of engineered materials that are critical for sustaining life in the modern age. These products are key components in many industrial processes, such as the production of steel, cement, glass, aluminum, copper, lead, and many others. It is estimated that 50 million tons of refractory products are produced and used around the world every year. Of this, an estimated 28 million tons of spent refractory is generated after use, much of which ends up in landfills all over the globe. By utilizing embodied energy methodologies, a complete sum of energy inputs can be mapped throughout …

Chronicles Of Film Tourism: An Integrative Review And Future Research Directions, Sangkyun Kim, Anton Klarin, Stijn Reijnders, Qijie Xiao, Nicky Van Es

Research outputs 2022 to 2026

This study triangulates historiographical and scientometric reviews to provide a systems perspective on film tourism. The three phases of historiographical analysis (exploration, growth, and maturity) correspond to the three clusters of scientometric analysis: (i) initial research exploring film tourism development and management, (ii) growing the field through studies on film tourists’ perceptions and practices, and (iii) reaching maturity through extending research into different cultural contexts and meaning-making processes. Based on the analysis, we offer a set of directions for future research, including the need to integrate sustainability research and film tourism studies in developing country contexts, as well as intertextuality …

Driving Factors And Spatio-Temporal Evolution Of Tourist Accident Clusters In Chinese Outbound Tourism, Chaowu Xie, Kun Zhang, Jiangchi Zhang, Songshan Huang

Research outputs 2022 to 2026

The clustering of tourist accidents is a critical yet underexplored area of research. This study employs cluster theory and safety system theory to develop a comprehensive measurement tool for analysing tourist accident clusters, examining their spatiotemporal evolution patterns, and identifying the driving mechanisms from a holistic destination perspective. By analysing accident records of Chinese outbound tourists across 48 countries from 2011 to 2019, the findings reveal a reduction in disparities among tourist accident clusters (α-convergence effect) and a fluctuating trend over time. Spatial autocorrelation analysis identifies four distinct spatial distribution patterns: low-low (L-L), high-low (H-L), high-high (H-H), and low-high (L-H). …

Stakeholder Co-Design Of Sustainable Urban Pest Management Strategies, Elizabeth C. Lowe, Nathan J. Butterworth, Alexander Austin, Cameron Webb, Tanya Latty

Research outputs 2022 to 2026

The high levels of broad-spectrum insecticides used to manage invertebrates in many cities around the world has significant environmental and health impacts. Integrated pest management (IPM) is a framework for sustainable pest control practice, but uptake of IPM remains low in most cities. We used participatory action research with pest management industry stakeholders (practitioners, industry representatives, researchers and government employees) to identify key issues in urban pest management in Australia, set priorities for change and discuss collaborative solutions. Via an online survey and a face-to-face workshop, the participants identified the key themes of education and training, public awareness, environmental impacts …

Sustainable Textiles Through Electrospinning: Addressing Environmental Challenges, Nicole Sharples, Omid Doustdar, Miguel Carmona‐Cabello, Shayan Abrishami, Jose M. Herreros, Ali Sadaghiani, Karl D. Dearn

Research outputs 2022 to 2026

The textile industry, known for its significant environmental footprint, faces growing pressure to adopt sustainable practices. This review identifies the textile industry’s sustainability challenges and examines electrospinning as a promising technology to mitigate its environmental impact. Electrospinning enables the production of nanofibers with enhanced properties suitable for various textile applications, offering potential solutions to reduce resource consumption and waste generation. This review discusses the current uses of electrospinning in textile manufacturing and analyses emerging trends in textile recycling, emphasising the role of electrospinning in transforming waste textiles into value-added products. Prospects for advancing electrospinning techniques to achieve efficient textile recycling …

Can Environmentally Friendly Hotels Lead Customers To Green Behaviors? Evidence From The Stimulus–Organism–Response Model And Social Cognitive Theory, Saeid Nosrati, Mohtaram Rabbani, Razieh Sharifipur Shirazi, Ilkay Yorganci, Niusha Talebzadeh, Negin Bassirat

Research outputs 2022 to 2026

Purpose Using the stimulus–organism–response model and social cognitive theory, this study aims to examine how environmentally friendly hotels influence customers’ green patronage intention and green word-of-mouth. The study explored the mediating effects of hedonic value and utilitarian value and the moderating role of environmental concern. Design/methodology/approach Data were collected from 200 customers of environmentally friendly hotels in Iran and analyzed using structural equation modeling (SEM) with Mplus (version 8.3) software. Findings The results indicate that environmentally friendly hotels positively influence hedonic and utilitarian values, as well as green patronage intention and green word-of-mouth. Additionally, hedonic and utilitarian values partially mediate …

Breaking Down Seagrass Fragmentation In A Marine Heatwave Impacted World Heritage Area, Michael D. Taylor, Simone Strydom, Matthew W. Fraser, Ana M.M. Sequeira, Gary A. Kendrick

Research outputs 2022 to 2026

Marine heatwaves, and other extreme climatic events, are driving mass mortality of habitat-forming species and substantial ecological change worldwide. However, habitat fragmentation is rarely considered despite its role in structuring seascapes and potential to exacerbate the negative impacts of habitat loss. Here, we quantify fragmentation of globally significant seagrass meadows within the Shark Bay World Heritage Area before and after an unprecedented marine heatwave impacting the Western Australian coastline over the austral summer of 2010/11. We use a spatial pattern index to quantify seagrass fragmentation from satellite-derived habitat maps (2002, 2010, 2014 and 2016), assess potential predictors of fragmentation and …

Icipe Spotlight Magazine 2026, International Centre Of Insect Physiology And Ecology (Icipe)

icipe Newsletters

Spotlight is a metaphor of icipe’s desire to illuminate our scientific breakthroughs and the lives they touch. While we celebrate our contribution to the global knowledge hub, our deepest fulfilment is in witnessing how the knowledge and innovations transform lives and foster resilience.

Intelligent Dual-Energy X-Ray Absorptiometry (Idxa) And Small Animal Scanning As A Useful Tool To Measure Appendicular Bird Bone Mineral Density, Frank J. Dirrigl Jr., Samuel R. Buchanan

School of Integrative Biological & Chemical Sciences Faculty Publications

Accurately assessing the bone mineralization status of birds is crucial for poultry production and wild bird studies. We investigated the use of dual-energy x-ray absorptiometry (DXA) using Small Animal Measurement software to measure bone mineral content (BMC) and areal bone mineral density (aBMD) in excised radii of the willow ptarmigan (Lagopus lagopus) as a bird model. Our exploratory tests involved 117 scans to evaluate scanning parameters, scanning board types, bone stabilization, scan mode and size, and region of interest placement. Results indicated that mean BMC or aBMD were each significantly different when scanned on a forearm positioner board …

Energy Poverty, Economic Growth And Environmental Degradation In South Asia: Do Energy Resources Matter?, Memona Rasheed, Hooi Hooi Lean, Nam Foo

Research outputs 2022 to 2026

Developing countries can combat energy poverty by enhancing renewable energy and economic growth with minimal adverse environmental effects. In this paper, we present a comprehensive analysis of energy poverty, renewable energy, economic growth, carbon dioxide emissions, and urbanization (URB) in South Asian countries from 1990 to 2021, focusing on Pakistan, India, Bangladesh, and Sri Lanka. We employed econometric analysis techniques like Panel Pooled Mean Group (PMG)-Autoregressive Distributed Lag (ARDL), Fully Modified Ordinary Least Squares (FMOLS), and Dynamic Ordinary Least Squares (DOLS) to provide empirical results, as they enable panel data and control for endogeneity. The findings suggested that the fight …

Obesity Indices As Predictors Of Metabolic Syndrome: 1-Year Findings From A Peruvian Cohort Of Private Educational Institution Workers, Vicente A. Benites-Zapata, Daniel Fernandez-Guzman, Percy Herrera-Añazco, Enrique Moncada-Mapelli, Diego Urrunaga-Pastor, Carlos J. Toro-Huamanchumo

Research outputs 2022 to 2026

Background: Metabolic syndrome (MetS) is a common condition marked by abdominal obesity, insulin resistance, hypertension, and hyperlipidemia. It has significant health and economic impacts, highlighting the need for early predictive markers. Objective: To compare the prognostic performance of various obesity indices in the 1-year incidence of MetS among adult employees of a private educational institution. Design: Cohort study. Methods: A prospective cohort study was conducted using data from adults employed at a private educational institution in Lima, Peru. Participants were enrolled in 2019 and followed for 1 year to monitor anthropometric and biochemical parameters. The outcome was the development of …

When Affect Is Not Episodic: Extending Affective Events Theory Under Sustained Oversight, Cathrine Frost, Andrei A. Lux, Peter Galvin

Research outputs 2022 to 2026

Affective Events Theory explains how workplace emotions arise from discrete events and shape attitudes and behaviour. Drawing on a phenomenological study of 29 employees and 13 managers working within oversight saturated supervisory contexts in the post–Royal Commission Australian financial services sector, this paper extends Affective Events Theory by examining how affective experience unfolds when accountability is continuous, and discretion is constrained. Across dual-cohort findings, affect was not primarily anchored to identifiable events that resolved over time. Instead, participants described emotion as persistent and cumulative, produced through ambiguity and emotional restraint, and circulating across supervisory roles. Employees reported sustained interpretive effort …

Noongar Culture, Arts Learning, And Wellbeing Literacy In Early Childhood: A Case Study Of The Song Room’S Deadly Arts Early Years Program In Western Australia Government Primary Schools, Jason Goopy, Narelle Lemon, Megan Mcpherson, Libby Jackson-Barrett, Jacqueline West

Research outputs 2022 to 2026

This report presents findings from a case study examining how The Song Room's Deadly Arts Early Years program supports wellbeing literacy in early childhood education through engagement with Noongar culture, arts, and storytelling at Western Australia government primary schools. Conducted on Whadjuk Noongar Boodjar at Huntingdale Primary School and Orelia Primary School, the research was co-designed with Indigenous and non-Indigenous teaching artists, school communities, and The Song Room.

The study adopted wellbeing literacy — the capability to understand and intentionally use language about and for wellbeing across contexts — as its theoretical framework. Creative arts-based methods, including draw and tell …

Association Between Protein Intake From Animal And Plant Sources And The Presence Of Frailty In Community-Dwelling Australian Women, Trent Bozanich, Jack Dalla Via, Simone Radavelli-Bagatini, Cassandra Smith, Elsa Dent, Anthony Villani, Robin M. Daly, Michael Tieland, Jonathan M. Hodgson, Kun Zhu, Richard L. Prince, Marc Sim

Research outputs 2022 to 2026

We investigated the cross-sectional association between total, animal and plant protein intake with the presence of a multidimensional measure of frailty in older women. Participants included 1380 community-dwelling Australian women, mean (SD) age 75.1 (2.7) years. A validated food frequency questionnaire was used to determine total, plant, and animal protein intake. Frailty was assessed using a frailty index across numerous health domains (scored 0–1, ≥0.25 indicating frailty). Cross-sectional associations were analysed using multivariable-adjusted logistic regression models. In total, 275 (19.9%) women were identified as frail. A non-linear inverse association was observed between higher intakes of total protein and the odds …

Association Of Source-Specific Nitrate Intake With Inflammation And Cardiometabolic Health: A Cross-Sectional Study, Carolyn J. English, Catherine P. Bondonno, Liezhou Zhong, Anna Lohning, Nicola P. Bondonno, Dianne P. Reidlinger

Research outputs 2022 to 2026

This study aimed to explore whether health effects of dietary nitrate depend on its source, by investigating associations between plant and animal-sourced dietary nitrate groups with markers of inflammation and CVD risk factors. Among 100 non-smoking adults (mean age 49 ± 13 years, 31% male), dietary nitrate intake was assessed using food frequency questionnaire (n=100) and 3-day food diary (n=89), combined with nitrate food composition databases. Nitrate intake was classified into plant, naturally occurring animal and additive-permitted meat-sourced groups. Associations between source-dependent nitrate intakes and lipoprotein-associated phospholipase A2 (Lp-PLA2), C-reactive protein (CRP), fasting plasma lipids, anthropometry, and blood pressure were …

Enhancing Physical Performance With Ischemic Preconditioning: A Systematic Review And Meta-Analysis Of Moderators And Performance Outcomes, Yilin Zhang, Kai Xu, Hao Kong, Mingyue Yin, Chenghao Liu, Yun Xie, Liam Kilduff, Gustavo R. Mota, Olivier Girard

Research outputs 2022 to 2026

This study examined the effects of ischemic preconditioning (IPC) on physical performance, considering the influence of timing, warm-up (WU), participant characteristics, and IPC protocols structure. A total of 90 trials (1,439 participants) were retrieved from three databases and assessed using PICOS criteria. Multilevel meta-analyses with cluster-robust variance adjustments were conducted to calculate pooled effect sizes (Hedge’s g). Risk of bias and certainty of evidence were assessed using the RoB 2 tool and GRADE framework. IPC produced a trivial but significant improvement in physical performance (g = 0.13, P < 0.01), which persisted after excluding SHAM effects (g = 0.10; P < 0.01). Significant improvements were observed for maximum repetitions, time to failure, and power output, but not for jump ability, strength, or oxygen uptake. Comparable benefits were found for anaerobic (g = 0.15) and aerobic (g = 0.10) exercise, with greater effects in males and less-trained participants. Performance was further enhanced when IPC was followed by WU (g = 0.16), with the optimal IPC-to-WU interval being ~42 minutes. Without WU, the effective IPC window narrowed to 6–7 minutes. In practice, IPC can enhance physical performance, independently of sham effects, moderated by sex, training level, and WU. For competition or testing, the most effective strategy appears to be 3 or 4 × 5-min IPC protocol, followed by a 42-min interval, standardized warm-up, and endurance testing. For mechanistic studies, WU should be excluded, and testing conducted 6-7 minutes post-IPC. Future research should target women, middle-aged individuals, and elite athletes.