Digital Commons Network^™

Endogenous Processes Underlying Clock-Like Mutational Signatures, Teresa Druck, Rami I Aqeilan, C Marcelo Aldaz, Nicola Zanesi, Kay Huebner

Faculty, Staff and Student Publications

Wellcome Trust scientists have shown that “mutational signatures” in specific nucleotide contexts accumulate in genomes of mammalian tissues, providing clues to underlying causes of specific signatures. Analysis of cancer genomes has identified more than 50 single‐base substitution (SBS) signatures, with SBS1, SBS5, and SBS40 linked to aging and present in normal tissues. SBS1 results from cytosine demethylation, whereas SBS5 and SBS40 arise from unknown endogenous mechanisms. We hypothesized that loss of fragile‐site genes drives these two signatures. FHIT, located at FRA3B, is frequently deleted in cancers, and Fhit‐deficient mouse tissues exhibit a mutation profile resembling human SBS5. Data mining of …

Preclinical Ischemic Stroke Multicenter Trials (Prism) Collective Statement: Opportunities, Challenges, And Recommendations For A New Era, Cenk Ayata, Philip M Bath, Anna M Planas, Stuart M Allan, Johannes Boltze, Ryan P Cabeen, Claire L Gibson, Marilyn J Cipolla, Marcio A Diniz, Stefano Fumagalli, Fahmeed Hyder, Raymond C Koehler, Arthur Liesz, Sarah K Mccann, Tim Magnus, Louise D Mccullough, Emily S Sena, Simone Beretta, Jaroslaw Aronowski, Francesca Bosetti, Clinton B Wright, Patrick D Lyden, Lauren H Sansing

Faculty, Staff and Student Publications

Preclinical stroke research faces a critical translational gap, with animal studies failing to reliably predict clinical efficacy. To address this, the field is moving toward rigorous, multicenter preclinical randomized controlled trials (mpRCTs) that mimic phase 3 clinical trials in several key components. This collective statement, derived from experts involved in mpRCTs, outlines considerations for designing and executing such trials. mpRCTs offer advantages such as increased sample sizes, robust statistical design, incorporation of heterogeneity, and standardized protocols, but they face challenges in finding the right balance between standardization and heterogeneity, appropriate stroke model selection, and outcome measures, as well as the …

Intratumoral Bacteria Are Immunosuppressive And Promote Immunotherapy Resistance In Head And Neck Squamous Cell Carcinoma, Natalie L Silver, Jin Dai, Travis D Kerr, Jessica Altemus, Rekha Garg, Hannah Simmons, Tyler Alban, Laura Noel-Romas, Vladimir Makarov, David J H Shih, Shwetha V Kumar, Akeem Santos, Rehan Akbani, Adam Burgener, Mohammed Dwidar, Neil Gross, Andrew G Sikora, Elias J Sayour, Apollo Stacy, Christian Jobin, Timothy A Chan, Renata Ferrarotto, Daniel J Mcgrail

Faculty, Staff and Student Publications

Despite the promise of immune checkpoint blockade (ICB) in head and neck squamous cell carcinoma (HNSCC), mediators of response are poorly understood. To address this, here we analyzed oropharyngeal HNSCCs treated with neoadjuvant durvalumab (anti-PDL1) alone or in combination with tremelimumab (anti-CTLA4) from the CIAO clinical trial ( NCT03144778 ). We found that only the total abundance of intratumoral bacteria predicted ICB response, which was validated in multiple independent cohorts. High intratumoral bacteria abundance was associated with an immunosuppressive tumor microenvironment, characterized by an accumulation of neutrophils coupled with depletion of T cells and other adaptive immune cells. Experimental elevation …

Effects Of Biochar And Its Combination With Poultry Manure On Nitrogen Use Efficiency, Growth, And Yield Of Maize Under Different Nitrogen Fertilization Levels, Charles Lwanga Twagiramungu, Bobe Bedadi, Claver Ngaboyisonga, Concorde Nsengumuremyi, David Fangueiro

All Rsif Scholars' Publications

The effects of biochar, its combination with poultry manure with different nitrogen (N) levels on maize production on the Andosol and Nitisol was studied. Treatments were factorial combinations of three organic amendments (OA) (biochar - B, poultry manure - PM and B co-composted with PM - BPM) as main-plot factor and four N levels (0, 50, 75 and 100 kg N/ha) as sub-plot factor, laid out as split-plot design and replicated thrice at two sites: Kinigi with Andosol and Rwerere with Nitisol. At both sites, there was general increase in maize total N uptake, aboveground biomass (AGB) and grain yield …

Provoking Generative Ai Futures: Merging Theory And Praxis, Regina M. Luttrell Ph.D., Nick Bowman

Media Studies - All Scholarship

An accessible exploration of the myriad applications and challenges of generative AI for media and communication students, scholars, and practitioners alike.

The latest emergence of increasingly low-cost and scalable AI technologies presents a point of both celebration and concern for the contemporary media and information ecosystem. To this end, this edited volume gathers media and communications scholars and practitioners to engage in discussions and exchange ideas about current trends and developments in the field. Questions this volume asks include: What are the essentials of generative AI from a media and communication perspective? How has generative AI influenced research and scholarship? …

Excluded At The Root: Health Insurance Discrimination Against Hair Loss Conditions, Stacey A. Tovino, Tracy Hresko Pearl

Faculty Articles

No abstract provided.

Meat Is Dead, Melissa D. Mortazavi

Faculty Articles

The legal and cultural landscape surrounding meat in the United States is evolving as plant-based alternatives, GMO farm animals, and cell-cultivated meat move from test tube to table. While global demand for meat rises amid environmental, ethical, and resource challenges, cell-cultivated meat emerges as a scientifically viable alternative, offering identical nutritional value without the drawbacks of conventional livestock production. Despite federal regulatory approval from USDA and FDA, multiple states have enacted unusually restrictive laws—including bans— targeting cell-cultivated meat. These are motivated largely by economic protectionism, but also deeper cultural anxieties tied to tradition and identity. This Article argues that food …

Assessing Interactions Between Wild Host Plants And Stemborer Species In East Africa, Elie Ntirenganya, Venuste Nsengimana, Bonoukpoè Mawuko Sokame, Simon Boniface Boni, Yoseph Assefa, Janvier Uwayezu, Muluken Goftishu

All Rsif Scholars' Publications

Lepidopteran stemborers are among the most destructive pests of maize, sorghum, and sugarcane in Africa. Yet, data on their species composition and host range in Rwanda remain limited. This study provides the first comprehensive assessment of stemborer diversity, seasonal dynamics, and altitudinal distribution across eastern (low altitude), central (mid-altitude), and northern (high altitude) Rwanda. Surveys were conducted during both the rainy (maize growing) and dry seasons of 2023–2024, targeting infested maize fields and surrounding wild vegetation. A total of 2691 stemborer individuals were recovered from nine host plants, with 1474 (54.8%) from wild and fodder vegetation and 1217 (45.2%) from …

Dietary Responses Of Taste Receptor Genes To Plant-Based Diets In Farmed Fish: A Literature Review, Leonildo Dos Anjo Viagem, Jean Nepomuscene Hakizimana, Cyrus Rumisha, Brunno Da Silva Cerozi, Gerald Misinzo

All Rsif Scholars' Publications

Taste receptor genes, particularly those in the bitter (tas2r) and umami/sweet (tas1r) families, are critical in regulating chemosensory perception and feeding behavior, influencing fish’s acceptance of plant-based diets. This review synthesizes the current research on how these genes respond to plant-based diets in farmed fish. A comprehensive search of scientific databases, including Web of Science, Scopus, PubMed, and Google Scholar, was conducted to identify relevant studies. Plant-based diets modulate gene expression, receptor activity, and signaling pathways, enabling fish to adapt to alternative feeds. The findings of the studies analyzed in this review show that plant ingredients affect fish taste receptor …

Gut And Blood Microbiota Of Nile Tilapia (Oreochromis Niloticus) Reared With Lettuce (Lactuca Sativa) And Challenged With Escherichia Coli And Vibrio Cholerae In Aquaponic Systems, Ang´Elica Adiaç˜ Ao Jossefa, Leonildo Dos Anjo Viagem, Brunno Da Silva Cerozi, Sebastian Wilson Chenyambuga

All Rsif Scholars' Publications

Aquaponics, a bio-integrated system that combines fish farming and vegetable cultivation, has gained significant attention due to its rapid expansion and potential for sustainable food production. In aquaponics, along with plants and fish, both beneficial and harmful microorganisms are also present. This study was conducted to assess the microbiota diversity of Nile tilapia reared with lettuce in aquaponic systems, with a focus on food safety implications. A total of 144 Nile tilapia juveniles with an average initial weight of 30.7 ± 0.03 g were randomly stocked and allocated to three treatments, each with three replicates. In treatment one, the fish …

Effect Of Diets Containing Soybean Or Seaweed (Kappaphycus Sp.) On Growth, Feed Utilization And Nutrient Digestibility In Nile Tilapia (Oreochromis Niloticus), Leonildo Dos Anjo Viagem, Jean Nepomuscene Hakizimana, Cyrus Rumisha, Gerald Misinzo, Brunno Da Silva Cerozi

All Rsif Scholars' Publications

Aquaculture is rapidly expanding, with Nile tilapia (Oreochromis niloticus) being a key freshwater species. Rising fishmeal (FM) costs and environmental concerns have increased interest in sustainable alternative protein sources. This study evaluated the effects of diets containing soybean (SB) or Kappaphycus sp. on the growth, feed efficiency and nutrient digestibility of Nile tilapia (O. niloticus). Four dietary treatments were tested over a 62-day feeding trial: FM diet, a diet containing SB, Kappaphycus sp. (seaweed [SW]) and a natural meal (NM) serving as a negative control. Diets were formulated to be isoproteic (360 g/kg) and isocaloric (3600 kcal/kg). Growth performance, feed …

Effects Of Biochar Produced From Different Feedstocks And Organic Fertilizers On Soil Properties And Maize (Zea Mays L.) Production In The Northern Part Of Rwanda, Charles Lwanga Twagiramungu, Bobe Bedadi, Claver Ngaboyisonga, Concorde Nsengumuremyi, David Fangueiro

All Rsif Scholars' Publications

This study applied biochar derived from different local feedstocks and organic fertilizers (OF) on contrasting acidic soils, Andosol and Nitisol, in order to investigate their effects on selected soil properties and maize growth and yield parameters. A split plot design was employed, with treatments being factorial combinations of OF (control without OF (OF0), cow manure (OF1), chicken manure (OF2), and compost (OF3)) as a main plot factor and biochar (no biochar applied (B0), maize straw biochar (B1), eucalyptus biochar (B2), pinus biochar (B3)) as a sub-plot factor. An amelioration in soil properties was observed in treated plots compared to the …

Forecasting Future Adoption Rates Of Agroecological Innovations Using Machine Learning: Perspectives From Burkina Faso’S 45 Provinces From 2010 To 2020, Theodore Nikiema, Pamela Giselle Katic, Issaka Abdou Razakou Kiribou, Sylvain Kpenavoun Chogou, Eugene C. Ezin

All Rsif Scholars' Publications

Implementation of agroecological innovations tends to be long-term processes, withthe practices of one year often linked to those of previous years. However, previousstudies have focused on understanding drivers of adoption at farm level, with adoptionmeasured at a point in time. In this study, we use a decade of panel data from thePermanent Agricultural Survey in Burkina Faso from 2010 to 2020 and machine learningapproaches, to model adoption rates of agroecological innovations at the provinciallevel as an autoregressive process. This modeling approach allows us to exploit the timeseries nature of our dataset to forecast future adoption rates. Our results showcase thepotential …

Occurrence And Levels Of Multiple Veterinary Antibiotics In Broiler Chicken Processed And Sold In Southern Mozambique, Noemia Andre Mugabe, Jamal Kussaga, Abdulsudi Issa-Zacharia

All Rsif Scholars' Publications

Antibiotic residues in chicken meat pose a health risk to the general public, particularly to sensitive consumers. The aim of this study was to determine the level of antibiotic residues in on-the-market dressed chicken in the Southern Region of Mozambique. A total of 160 samples were randomly collected from eight markets located in five districts in the provinces of Maputo, Gaza, and Inhambane. The residues of β-lactams, quinolones, tetracyclines, and sulfonamides in chicken breast muscle were separated, identified, and quantified using high-performance liquid chromatography (HPLC), coupled with ultraviolet light (UV–VIS). Results revealed that 26.9% of the analyzed samples displayed detectable …

Osteogenic Differentiation Properties Of Blgfs/Odex Hydrogel Doped With Collagen Derived From Chambo Tilapia Fish Skin, Bhahat Lawlley Zimba, Xiaohan Yang, Mwemezi Rwiza, Elingarami Sauli, Shenqi Wang

All Rsif Scholars' Publications

Background

Bone defects caused by trauma, congenital disorders and degenerative diseases such as osteoporosis remain a major clinical challenge, especially in developing regions. Although bone grafting is the current gold standard for treating bone defects, it faces limitations, including donor site morbidity, limited availability and immune rejection. This study developed and evaluated a novel hybrid biomaterial composed of bovine β-lactoglobulin fibrils (BLGFs), oxidized dextran (ODEX) and collagen (COL) extracted from Chambo tilapia fish skin for bone tissue engineering applications.

Methods

BLGFs were synthesized from whey protein, while ODEX was produced by oxidizing dextran. Three biomaterials–COL, BLGFs/ODEX and BLGFs/ODEX/COL–were synthesized and …

Functionalized Blgfs/Odex Hydrogel Scaffold Incorporating Oreochromis Lidole Skin Collagen For Bone Tissue Regeneration, Bhahat Lawlley Zimba, Xiaohan Yang, Mwemezi Rwiza, Elingarami Sauli, Shenqi Wang

All Rsif Scholars' Publications

Background: Bones provide support to the body and protect vital internal organs. Bones have the capacity to repair minor defects, but this ability is substantially impaired in large defects. Mineralization, which is an essential stage of osteogenesis during bone defect repair, occurs through the deposition of calcium phosphate crystals to form the structural framework of bone tissue. In the present study, BLGFs/ODEX/COL hydrogel scaffolds were synthesized and characterized to determine their osteogenic differentiation capabilities. The hydrogel scaffolds were also applied to bone defect in SD rats and the regeneration of bone tissue was assessed. Methods: Whey protein was used to …

Access Over Impact: Reorienting Oira’S Meetings With The Public, Griffin S. Kenny

Case Western Reserve Law Review

No abstract provided.

When To Bite Back: Queerness, Resistance, And Reclamation Of The Monstrous Identity In Andrew Joseph White’S Hell Followed With Us, Aster J. Mehigan Mx.

English

Who gets the right to define morality? Who gets to define what is monstrous? Frankenstein’s Creature, the vampires of Lost Boys, The Craft’s head witch Nancy Downs; these characters follow a pattern through the decades of those alienated from society, in this case being written with queer subtext, being deemed as monstrous. These queer monsters become the villains of the story, causing chaos, death and destruction in their wake, seeking revenge against a world that ostracizes them. The 2022 dystopian YA horror of Hell Followed With Us by Andrew Joseph White challenges the villanized monstrous identity through its transgender protagonist …

Dog Waste Creates A Preference-Performance Mismatch For Urban Dung Beetles, Erin L. Mccullough, Beatrice M. Altopp, Daniel F. Kellogg, Madelyn S. Mcinnis, Luna J. Pagan

Biology

Urban environments expose animals to novel conditions that may favor behavioral flexibility but can create preference-performance mismatches when attractive resources are associated with reduced reproductive success. Dung beetles are often considered dietary generalists, yet little is known about how they respond to altered food availability in human-dominated habitats. We combined field surveys and laboratory experiments using two domestic dung types to examine diet preference, maternal provisioning, and offspring performance in the urban dung beetle Onthophagus orpheus. Beetles strongly preferred dog dung over cow dung, and offspring reared on dog dung developed faster and emerged as larger adults. Despite these apparent …

Clinical Challenges Associated With Enhancing Hypertension Among The Underserved African American Population, Ashley Richardson

Doctor of Nursing Practice (DNP) Scholarly Projects

Background 

African Americans face a significantly higher prevalence of hypertension, tend to develop complications at younger ages, and have disproportionately higher mortality rates. Key social determinants of health (SDoH)—including food deserts, unequal access to healthcare, and geographic disparities in healthcare resources—contribute to these ongoing inequities. Addressing both systemic and individual barriers is crucial for effective hypertension management.

Objective

This study aims to assess the effectiveness of a culturally relevant, community-based intervention designed to reduce both systolic and diastolic blood pressure among Black adults with uncontrolled hypertension.

Methods 

Quality improvement (QI) initiatives typically employ multicomponent, equity-focused models. The primary components of …

The Postsynaptic Scaffolding Protein Sapap3 Shapes Mitochondrial Activity: The Case Of Huntington's Disease, Patrícia Coelho, Ildete Luísa Ferreira, Ana Sofia Lourenço, Daniela Marinho, Sandra Isabel Anjo, Zongwei Fang, Lígia Fão, Sandra I. Mota, Philippe J. Mas, Mário Carvalho, Rui Jorge Nobre, Carina Henriques, Joana Fraga, Dongqing Wang, Sandra Macedo Ribeiro, Luís Pereira De Almeida, Patrícia Monteiro, Isaura Simões, Darren J. Hart, Bruno Manadas, João Peça, Pedro Castanheira, A. Cristina Rego

Biological Sciences Faculty Publications

Postsynaptic scaffolding protein SAP90/PSD95-associated protein 3 (SAPAP3) modulates cortico-striatal signalling and regulates the maintenance of synaptic structure. Notably, SAPAP3 defects have been reported in several human psychiatric disorders that share pathophysiological features with Huntington’s disease (HD), a neurodegenerative disorder characterized by the expression of mutant huntingtin (mHTT) and marked dysfunction of cortico-striatal synapses and mitochondria. However, the role of SAPAP3 in mitochondrial function and HD pathophysiology remains unexplored. SAPAP3 was extracted from striatal synaptoneurosomes and analyzed by SWATH-MS proteomics to identify potential interactors, revealing SAPAP3 association with several mitochondrial proteins, particularly Mic60. These data were further complemented with proximity ligation …

Automation And Habitat Development For A Space Based Marine Life Environment, Logan Trimmer, Alexander Hang

Harrisburg University Other Works

This project was a cross-collaboration between the Environmental Sciences and Advanced Manufacturing and Robotics programs for the company Monolith Space.

The goal of this project was to design an autonomous system to be able to track qualities of water in an aquaculture system designed to be sent to space.

Rhetoric Of Opportunity: An Analysis Of Gullah Geechee Histories Through The Lens Of Counterstory, Xaria Arthur

Graduate Studies Theses and Dissertations 2026

Counterstory as a method/ology has been used in a variety of disciplines to share experiences of individuals and communities whose voices have been historically silenced or suppressed. Utilizing this methodological framework, my study shares the stories of six Gullah Geechee descendants with ancestral roots in the unincorporated community of Nesmith in Williamsburg County, SC. Treating these minoritized and individual stories as experiential knowledge, I trace the counterstories as topoi to introduce and develop the theoretical concept that I call the rhetoric of opportunity, which refers to memories or stories that have come to motivate individuals toward or away from …

It's All The Same, Lauren A. Bedevia

Graduate Studies Theses and Dissertations 2026

It’s All the Same is a collection of short stories that center around lesbian characters. The stories explore themes such as ambition, morality, karma, mental health, exploitation, and relationships. The collection opens with “Forget Her Inc.,” a short story that juggles an ensemble cast of lesbians and examines whether memory eraser therapy is an ethical practice for treating a broken heart. “It’s All the Same,” the titular story, uses lyricism to present the parallels between two women in terms of family dynamics, interests, and attachment styles; the story explores a loving friendship between a lesbian and a straight woman.

“Fun …

Carbaryl Exposure Alters Gut Microbiome Composition Without Affecting Morphometrics In Eastern Purple Martin (Progne Subis Subis) Nestlings, Stephanie A. Gaspar

Graduate Studies Theses and Dissertations 2026

Eastern purple martins (Progne subis subis) rely entirely on human-provided nesting structures throughout their breeding range in eastern North America. To control haematophagous mites that commonly infest these nests, many landlords apply the carbamate insecticide, carbaryl. Although effective at reducing ectoparasite populations, carbaryl exposure may have unintended effects on the gut microbiome, growth, and condition of developing birds. This study examined the effects of carbaryl exposure on gut microbial communities and morphometric condition in wild nestlings. Nests were experimentally treated with carbaryl, and nestling morphometrics and fecal microbiomes were compared between treated and untreated groups at 18 days …

Boys Will Be Empathetic: How Does Media Literacy Education Impact Adolescent Boys’ Empathy?, Karley A. Betzler

All Electronic Theses and Dissertations

Many adolescent boys are using social media each day for a variety of reasons. How social media is being utilized by these students, what they are being potentially exposed to online, and how it is impacting their offline lives has raised questions and concerns for many educators. This literature review explores how teaching media literacy skills to adolescent boy students impacts their empathy. This review specifically focuses on research done around the impact of social media use on the development of empathy and masculinity, how the impacts of boys’ exposure to the manosphere are being felt and perpetuated in schools, …

How To Stop Mass Shootings Without Violating The Second Amendment: Designing Regulations To Prevent Acts Of Mass Violence That Pass The 6:3 History And Tradition Test, Robert D'Alessandro

Cardozo Journal of Equal Rights & Social Justice

The article proposes that states can constitutionally implement ammunition possession limits to reduce mass violence by aligning such laws with the "history and tradition" test established by the current Supreme Court. It argues that modern caps on ammunition are analogous to early American gunpowder restrictions, which were enacted for public safety to prevent catastrophic explosions in populated areas.

The Inward Gaze: Byung-Chul Han's Achievement-Subjectivity In David Foster Wallace's Short Fiction, Ivan Marquez

All Student Theses and Dissertations

David Foster Wallace stated that the aim of his fiction and art was to reflect what it meant to be alive and form a relationship with the reader. In "E Unibus Plurum", Wallace posited that the tenets of Post-Modern fiction were once used to criticize the hypocrisies of the 1950's and 60's American had been subsumed by television and were no longer effective tools of criticism. Wallace argues for a move away from irony and stunt pilotry of postmodern fiction towards a fiction which espoused "single entendre values" (Wallace 192). While Wallace advocated for a return to more traditional story-telling, …

Intratracheal Interleukin-10 Administration Reduces Acute Lung Injury Induced By Sulfur Dioxide Exposure In Mice, Amy J. Nelson, Todd A. Wyatt, Bradley W. Richmond, Belinda Diaz, Angela Gleason, Michael J. Duryee, Geoffrey M. Thiele, Ted R. Mikuls, Jill A. Poole

Journal Articles: Environmental, Agricultural & Occupational Health

Therapies capable of reducing acute high-consequence airborne chemical exposure-induced lung disease are lacking. This study seeks to determine the therapeutic potential of interleukin (IL)-10 administered following acute sulfur dioxide (SO₂) exposure in mice. C57BL/6 male and female mice were exposed to SO₂ gas at 125 ppm for 4 h and subsequently treated 2-3 h later with IL-10 (1 μg) or vehicle control by intratracheal instillation for a total of 3 doses over 2 days with comparisons to sham-treated mice (N = 20 mice/group). IL-10 treatment reduced SO₂-induced weight loss with corresponding reductions in SO_{2 …}

Investigating The Potential Neurotoxic Effects Of Cell-Free Factors By Batrachochytrium Dendrobatidis On Locomotion In Xenopus Laevis, Anna Lennon, Laura K. Reinert, Louise A. Rollins-Smith, Will Best, Chase T. Kinsey Phd

Funded Scholarship

Chytridiomycosis is a contributor to amphibian population declines. Diseased amphibians show symptoms of lethargy and loss of righting reflexes, likely due to ion imbalance across the skin. However, it is possible developing zoosporangia release toxins that affect neuromuscular activity. Using Xenopus laevis as a model, we hypothesized that locomotor performance would be affected by injection of Bd supernatant factors. X. laevis were injected and then filmed performing a swimming escape response with high-speed cameras at 4 hours, 24 hours, and 1- week post-injection. Average maximum swimming velocity and escape latency were digitized using high speed video. Despite no difference in …