Digital Commons Network^™

Dusp8 Gene Expression Is Increased Upon The Stimulation Of Hek293 Cells, Oanh Vo, Lukas Macha

Student Summer Scholars Manuscripts

Dual specificity phosphatase 8 (DUSP8) is one of the phosphatases that oppose the activity of mitogen-activated protein kinases. Previous mouse studies have demonstrated the functional role of DUSP8 in regulating cardiomyocyte growth. However, it is still unclear how the activity of DUSP8 is regulated, and little is known about the regulation of DUSP8 at the gene level. The goal of this study is to determine whether the DUSP8 gene is immediately transcribed upon MAPK activation in the cells. Here, we found that the expression of the DUSP8 gene was significantly increased after stimulation of cells for 30, 60, 90, and …

Dna Methylation Variation In Eastern Diamondback Rattlesnakes (Crotalus Adamanteus) On A Georgia Barrier Island, Megan Hoog

College of Graduate Studies: Theses & Dissertations

The Eastern Diamondback Rattlesnake (Crotalus adamanteus, EDB) is the largest rattlesnake in the world and is thought to be declining in many portions of its range. Genetic venom studies in the Southeast United States have shown evidence of fine-scale differences within two main geographic groups on Jekyll Island. My objective is to expand on this finding to determine if epigenetic mechanisms, specifically DNA methylation, varies among different populations of EDB in similar environments, age classes, and sexes. I collected EDB blood samples through a partnership with the Jekyll Island Authority and screened 44 individuals for epigenetic variation from …

Sex-Specific Regulation Of Mash And Hepatocellular Carcinoma: A Role Of Sphk2 In Females, Bria Pierre

Theses and Dissertations

Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) is characterized by excessive fat accumulation in the liver, with metabolic dysfunction-associated steatohepatitis (MASH) representing a more severe form that can progress to cirrhosis or hepatocellular carcinoma (HCC). The sphingosine kinases and the S1P signaling pathway are key regulators of MASH and HCC progression. While much is known about the mechanisms contributing to MASH-HCC in males, less is known for females. Nevertheless, we previously identified sphingosine Kinase 2 (SphK2) to protect females from MASH-driven HCC. This study first performed differential gene expression analysis on human liver cohorts to examine sex-specific differences in gene expression …

The Diseased Extracellular Matrix In Chronic Obstructive Pulmonary Disease Modeling, Leigh-Ann M. Antczak

Theses and Dissertations

Chronic obstructive pulmonary disease (COPD) is currently the fourth leading cause of death in the world, with an increasing prevalence as the world’s population ages. In the distal regions of the lungs, COPD is characterized by progressive emphysema due to an imbalance of proteases that continuously degrade essential extracellular matrix (ECM) proteins within the alveolar walls, causing fatal tissue impairment. This dissertation examines the unique properties of this diseased lung ECM in two parts: (1) As a key constituent in inflammatory signaling that leads to increased inflammation and progression of COPD, and (2) as a tool in three-dimensional (3D) in …

Breaking Ground: The Impact Of Construction On Nutrient Loads In An Urban Stream, Brody N. Wilson, Shannon Speir, Alana G. Strauss, Caroline G.T. Anscombe

2025 Research Poster Competition

Degradation of water quality is primarily driven by human activities, such as urbanization. Urban Stream Syndrome (USS) can be characterized by the negative physical, chemical, and biological changes to water quality as a result of urbanization. One key effect of USS is the input of excess nutrients, which can drive unwanted macrophyte and algal growth. New construction is prevalent in urbanizing areas, resulting in the influx of nutrients, sediments, and other materials into stream ecosystems. However, few studies have examined the impacts of active construction on water quality. As such, we do not understand how construction affects nutrient transport downstream. …

Intercellular Stress Generation During 1d Collective Migration Of Cancer Cells, Logan Waddle, Jian Zhang

2025 Research Poster Competition

Physical forces drive many cellular processes such as migration and proliferation. A metastatic tumor will have invasive strands/chains that extend from the main tumor. These chains of cells collectively migrate away from the main tumor to establish new colonies elsewhere in the body. However, how physical forces drive this collective invasion and the required energy for this process is not well understood. Invasion of a metastatic tumor to surrounding tissues leads to a majority of cancer-associated death and is often a collective effort among cells, it is therefore important to fully understand the process behind collective cancer invasion.

The goal …

Effect Of Ventilation And Wearing A Facemask In Reducing Indoor Aerosol Transmission, Clothilde Brochot, Ali Bahloul

Articles dans des actes de congrès

The COVID-19 pandemic has significantly disrupted research in respiratory protection and transmission routes. Indeed, the pandemic has highlighted a number of issues, including those related to the performance and use of respiratory protective equipment such as masks and ventilation. A plethora of commercial and homemade masks have widely appeared during the pandemic, although they are not yet fully regulated in performance and fit test. However, with regard to the source reduction process, testing facemasks not at the inhalation, but at the source (exhalation) offers a new perspective on how to prevent particle emissions. Different means of transmission reduction are measured …

From A Spectrum To Diagnosis: The Integration Of Raman Spectroscopy And Chemometrics Into Hepatitis Diagnostics, Muhammad Kashif, Hugh J. Byrne

Articles

Hepatitis, most importantly hepatitis B and hepatitis C, is a significant global health concern, requiring an accurate and early diagnosis to prevent severe liver damage and ensure effective treatment. The currently employed diagnostic methods, while effective, are often limited in their sensitivity, specificity, and rapidity, and the quest for improved diagnostic tools is ongoing. This review explores the innovative application of Raman spectroscopy combined with a chemometric analysis as a powerful diagnostic tool for hepatitis. Raman spectroscopy offers a non-invasive, rapid, and detailed molecular fingerprint of biological samples, while chemometric techniques enhance the interpretation of complex spectral data, enabling precise …

Bsn Direct Entry, Nursing

Program Revisions/Additions

No abstract provided.

Exercise Science Physical Therapy, Ksr

Program Revisions/Additions

No abstract provided.

Exercise Science Pre-Athletic Training, Exer

Program Revisions/Additions

No abstract provided.

Bs Clinical Laboratory Science, Biological Sciences

Program Revisions/Additions

No abstract provided.

Wiring Attachment, Alexander K. Bacon

Theses and Dissertations

This thesis began with a question: can diagrams create understanding, or only reflect it? Through paintings that layer automotive wiring schematics with family photographs, I explore how the human attachment system resists easy mapping. Using digital tools like Photoshop and scanning, alongside analog methods such as papier-collé and photo transfer, I examine how systems built for clarity—like wiring diagrams—contrast with the layered, often messy realities of human relationships. Drawing from attachment theory’s empirical foundation, rather than the metaphorical leanings of psychoanalysis, my work investigates how emotional bonds form, falter, and repair over time. Material loss and layering are not flaws, …

Smear Layer Removal By Irrigation Adjuncts: A Scanning Electron Microscopy Study, Triet D. Vuong

Theses and Dissertations

Purpose: This study aimed to compare the intracanal smear layer removal ability of two adjunctive irrigation modalities: the AdvErL EVO laser, the GentleWave^® Procedure, and their combination, after minimally invasive canal instrumentation.

Methods: Forty molars were accessed, the mesiobuccal canals of maxillary and mandibular molars were prepared up to apical size 25.04 using rotary instrumentation, then irrigated using: conventional needle irrigation (CNI), laser-assisted irrigation (LAI), the GentleWave^® Procedure (GWP), and LAI followed by GWP (LAI+GWP). The teeth were then decoronated and split longitudinally. One-half of each root was selected for preparation and analysis under scanning electron microscope. Blinded …

The Bacteriostatic, Regenerative, And Immunomodulatory Properties Of Extracellular Matrix Particles For Lung Injury, Keera P. Rhoads

Theses and Dissertations

Acute respiratory distress syndrome (ARDS) is a prevalent, life-threatening lung condition, affecting nearly 200,000 Americans annually, with a 40% international mortality rate. There is no cure for ARDS, and current pharmacological treatments have limited effectiveness. Symptoms can be mitigated with mechanical ventilation, though this often leads to ventilator-induced lung injuries (VILI) and puts critically ill patients at risk of infections, including ventilator-associated pneumonia (VAP). A promising therapeutic is the extracellular matrix (ECM), a complex network of structural proteins and bioactive molecules that has been shown to have anti-inflammatory properties and prevent fibrosis. We aim to utilize the regenerative and immunomodulatory …

Ecological Interactions Of Large Mammals In Luangwa, Zambia, Jahzara Osborne, Amelia Villaseñor

2025 Research Poster Competition

"The connection between mammalian behavior in national parks and human-driven environmental change is tenuous. National parks are often considered the last vestiges of natural space, yet they are created and maintained by humans. South Luangwa National Park (SLNP) within the southern East African Rift is a microcosm of this global conundrum and is also a region where stable isotope data is sparce. Understanding the mammalian diets of mammals in the SLNP via carbon and oxygen stable isotopes in teeth will provide a means of understanding variation in one aspect of mammal behavior that may be affected by changing animal-human relationships. …

Glucose Limitation And The Second Glycolysis Peak In Stimulated Macrophages, Melih Karabacak

2025 Research Poster Competition

Previous studies have shown that individuals exposed to fever temperatures are much more likely to combat infections compared to those who are at normal body temperatures. However, in recent years, achieving fever responses has gotten much more difficult. Fortunately, in Dr. Durdik’s lab, it has been seen that effective fever temperatures can be generated artificially. The primary focus of the Durdik lab is an understudied field: the relationship between macrophage activity and fever temperatures. One key piece of information discovered from this research is the pattern of macrophages to go through different levels of glycolysis and mitochondrial respiration, with the …

Nests With Higher Thermal Variation Produce Fewer Fledglings With Larger Bills Than Higher Temperature Nests, Salvador Barraza-Del Barco, William Kirckpatrick, Sarah Heissenberger, Sarah Durant

2025 Research Poster Competition

Warmer temperatures and increased temperature variability are predicted in the future due to climate change. Phenotypic outcomes of avian nestlings are heavily impacted by nest temperature. We examined metabolic rates and growth of Eastern Bluebird nestlings subjected to increased mean temperature and increased temperature variability within the nest during development.

Effect Of Vibration Isolation During Transportation On Beef Sirloin Subprimal And Steak Quality, Kylie Nevada Krueger

Electronic Theses and Dissertations

The objective of this study was to determine the influence of vibration during transportation on beef sirloin water holding capacity, color, and tenderness. USDA Choice center-cut sirloin top butts (n = 45 cases) were collected at a commercial processing facility and randomly allocated to one of three treatments 1) regular pallet driven 241 km (Reg241), 2) regular pallet driven 644 km (Reg644), and 3) vibration isolation pallet driven 644 km (Iso644). All subprimals were transported at -2.2 °C (d0) and stored for 10 days to mimic industry practices for product aging. Following storage (d11), one subprimal per case was evaluated …

Polarimetric Capture And Differentiable Rendering, Katherine Anne Salesin

Dartmouth College Ph.D Dissertations

Many scientific fields rely on the capture and modeling of light to extract underlying information about the world. Often, more information can be extracted by capturing more about the nature of the light, such as its spectral shape or polarization state. While polarization is a relatively unexplored topic in computer graphics, when used in tandem with other recent advancements in the field it has enormous potential to improve both forward and inverse models in other scientific disciplines. We demonstrate this potential in two distinct settings in this thesis.

First, we apply the capture of polarized light to an inverse problem …

Sentence Determination As A Function Of Sex Offender Gender, Kyle Gamache, Matt Zaitchik, Judith Platania, Willem Pontbriand

Arts & Sciences Faculty Publications

The current study examines perceptions of dangerousness and sentencing recommendations for female sex offenders (FSOs) compared to male sex offenders (MSOs). Participants (N = 82) were randomly assigned to read one of three vignettes depicting a sexual offense committed by a male, female, or male-female co defendant pair. Key individual difference variables—Need for Cognition (NFC) and Gender Role Beliefs (GRB)—were assessed to examine their influence on sentencing and attributional judgments. NFC emerged as a significant moderator: participants with low NFC assigned longer sentences to FSOs, whereas high NFC participants gave longer sentences to MSOs. Participants endorsing traditional gender role beliefs …

Diversifying Ecology Education For Everyone Through More Inclusive, Interdisciplinary, And Accessible Teaching, Loren B. Byrne, Emily S. J. Rauschert, Vikki L. Rodgers, Gillian Bowser, Et Al.

Arts & Sciences Faculty Publications

Educating more students about ecology and its beneficial applications to societal issues is urgent yet challenging. To address this challenge, diversifying ecology education is a key way to make ecology more inclusive, accessible, and interdisciplinary for more people than ever. Advancing this goal requires ecology educators to develop a more expansive view of (1) how to engage more diverse undergraduate students in ecology courses, especially those from historically underrepresented groups and non-majors, (2) the interdisciplinarity of content in those courses, and (3) the learner-centered pedagogies used to engage students. We suggest ways that ecologists can advance “ecology education for everyone” …

Rightly Dividing Jus Ad Bellum And Jus In Bello In The Israel/Hamas War, Jeffrey F. Addicott

Faculty Articles

The purpose of this paper is to: (1) review the applicable rule of law as it relates to both the initiation of warfare and the use of armed force in the execution of that warfare; and (2) apply those legal standards to the facts associated with the Israel/Hamas conflict. While this may seem an easy task – to set out unanimity in the international community – it is not. The inability to set bright lines of distinction between aggressors and those acting in self-defense is not just a failure in definition or even understanding, it is a failure to legitimize …

The Research Examining Factors Associated With The Opioid Crisis Among Urban Adults (Refocus), Mona K.M. Goggins, Paige Orphé, Jovonna Atkinson, Brittany Miller-Roenigk, Kaylee A. Palomino, Danelle Stevens-Watkins

Educational, School, and Counseling Psychology Faculty Publications

Introduction: Deaths due to opioid-related overdoses have increased across the United States. Specifically in the state of Kentucky, African Americans are the only population that continues to experience an increase in opioid-related deaths. Previous literature highlights African Americans having unique drug use patterns due to systematic barriers and how these barriers limit access to protective factors and substance use treatment. The current study aims to specify patterns of African American non-medical prescription opioid use (NMPOU) and how these patterns may differ based on biological sex and generational cohort. Also, this study plans on using this data on patterns to inform …

Commencement Program, 2025, Cuny John Jay College Of Criminal Justice

Reports and Publications from John Jay College

Commencement Programs are handed out at each graduation. They include information on honorary degrees, awards and prizes and candidates for degree. However, they cannot be considered an official record of conferring of degrees.

Impact Of Substrates And Deposition Time On Bacteria Associated With Saliva Samples And Its Implication On A Bacterial Signature-Based Body Fluid Identification Method, Olisdey Galano Cruz

Master of Science in Forensic Science Directed Research Projects

Traditional serological methods for body fluid detection are limited by poor specificity and sensitivity, lacking confirmatory capability and thereby increasing the risk of false positives that may compromise the integrity of forensic investigations. While molecular methods such as mRNA, miRNA, and DNA methylation offer improved specificity, they are often hindered by marker instability and operational complexity in routine forensic workflows. Bacterial signature profiling has emerged as a promising alternative, offering enhanced stability, abundance, and compatibility with existing DNA workflows. However, its forensic implementation requires further validation. This study evaluated the effects of substrate type and storage time on the salivary …

Qualitative Method Validation Of Acid/Neutral Drugs Carisoprodol, Meprobamate, And Naproxen Using Liquid Chromatography-Tandem Mass Spectrometry, Kate O. Martin

Master of Science in Forensic Science Directed Research Projects

Meprobamate, carisoprodol, and naproxen are acidic/neutral drugs widely prescribed for muscle relaxation and inflammatory conditions, with frequent detection in forensic toxicology casework due to extensive clinical use. A method to qualitatively evaluate these compounds was developed to unify acid/neutral drug screening. This study incorporated blank blood, antemortem blood, postmortem blood, urine, and liver homogenate as representative biological matrices. Method validation was conducted in accordance with ANSI/ASB Standard 036 for Forensic Toxicology, encompassing key parameters including limit of detection (LOD), endogenous interferences, potential interferences, carryover, processed sample stability, and ionization suppression/enhancement. The validation was performed using the internal standards carisoprodol-D7, meprobamate-D7, …

Novel Time-Since-Deposition Signatures For Canine Blood; Implications For Animal Cruelty Investigations, Alysia M. Townsley

Master of Science in Forensic Science Directed Research Projects

Dogfighting is a felony in all 50 states and is often tied to other organized crime activities including gambling, narcotics, and weapons trafficking. Because of the clandestine nature of dogfighting crimes, it may be necessary to determine the age of blood samples from suspected events, not just the source. In this study, flow cytometry was used to identify cellular autofluorescence signatures for estimating TSD (time-since-deposition) in canine blood deposits up to 6 months old. To accomplish this, blood was taken from canines of various chronological ages and breeds, then deposited to create TSD samples ranging between 1 day and 180 …

Development Of Novel Signatures For Determination Of Cell Types In Blood And Saliva Mixtures, Caitlin Lydon

Master of Science in Forensic Science Directed Research Projects

Determination of tissue source and the time at which a biological material is transferred from its source to another surface, known as the time-since-deposition (TSD), would assist forensic analysts by providing crucial context for DNA profiles generated from biological evidence. Many methods for cell identification have been explored using biomolecule markers such as mRNA, miRNA, and differential DNA methylation. Forensic laboratories have also developed methods for TSD estimation that focus on degradation of biomolecules. Biomolecules such as mRNA or miRNA degrade at different rates due to some being more stable than others. The ratio of stable degrading biomolecules, therefore, can …

History In The Making (Volume 18), Csusb - Alpha Delta Nu Chapter Of The Phi Alpha Theta National History Honor Society

History in the Making

No abstract provided.