Digital Commons Network^™

Myb And Hif1Α Crosstalk Drives Hypoxia-Induced Transcriptional Reprogramming And Adaptive Signaling Alterations In Pancreatic Cancer, Shashi Anand, Kunwar Somesh Vikramdeo, Mohammad Aslam Khan, Ajay Pratap Singh

Cell and Molecular Biology Publications

Pancreatic cancer is an aggressive malignancy, characterized by extensive desmoplasia and a hypoxic tumor microenvironment that contributes to therapy resistance. MYB, a proto-oncogene encoding a transcription factor, plays a crucial role in pancreatic tumor growth and metastasis. Recently, we also revealed a role of MYB in hypoxic survival of pancreatic cancer cells by promoting metabolic reprogramming through interaction with HIF1α, modulating its expression and binding to glycolytic gene promoters. In this study, we investigated how hypoxia influences the genome-wide occupancy of MYB using chromatin immunoprecipitation sequencing (ChIP-seq), and whether this effect is modulated by its interaction with HIF1α. In addition, …

Bowel Regimen Protocol Implementation For Pediatric Patients Following Cardiac Surgery, Eryn Watson

Dissertations

Problem: Pediatric patients with congenital heart disease (CHD) are at an increased risk of constipation following cardiac surgery, which contributes to complications and delays in recovery. Standardized protocols are effective tools for prophylactic management of constipation. However, there is no current standardized protocol in place for postoperative bowel management in a pediatric cardiac intensive care unit (CICU).

Method: This quality improvement project (QI) used a descriptive design with pre- and post-implementation data collection to evaluate the effects of a postoperative bowel regimen protocol on constipation rates and compliance with ordering prophylactic bowel regimens. Convenience sampling was used to identify patients …

"...Anything My Friend Shares, I Would Want To Support Them By Clicking On It": Co-Designing Story-Based Interventions Against Clickbait For Teenagers, Ankit Shrestha, Audrey Flood, Bryson Hackler, Mahdi Nasrullah Al-Ameen

Computer Science Student Research

Teenagers' lack of digital sophistication and cyber hygiene makes them vulnerable to social engineering attacks, especially as they start using social media. Clickbait, one of such attacks, is primarily performed through social media to trick users into clicking on malicious links. With teenagers' increasing use of social media, clickbait poses a substantial threat to their online safety. The existing online safety measures for teens mainly focus on parental mediation, which can be perceived as restrictive and privacy-invasive. To this end, researchers recommended empowering teens to deal with online risks. In order to design such interventions for clickbait, we conducted co-design …

Evaluation Of Diy Pest Management Strategies For The German Cockroach (Blattella Germanica) In The Presence Of Vitamin K Precursors And Synthetic Amorphous Silica, Mohamed S. Shaik

LSU Master's Theses

The German cockroach, Blattella germanica (Linnaeus) is a globally distributed indoor dwelling pest of medical concern. Insecticides are the preferred method of cockroach management due to their low cost, quick action, and availability. However, after continuous use, the development of insecticide resistance has emerged as a challenge for the pest management industry to effectively eliminate German cockroaches. Integrated pest management (IPM) is a sustainable framework which includes the use of glue board traps, vacuuming, in combination with different insecticide formulations such as sprays, gel or dust baits, and insecticide rotation has been successful for the management of German cockroaches. This …

Maternal Hyperleptinemia Alters Placental Transcriptome And Progesterone Regulation Without Affecting Early Equine Fetal Growth, Lucinda L. Thorburn

LSU Master's Theses

While consequences of equine obesity are well defined, the extent to which persistent hyperleptinemia and accompanying leptin (Lep) resistance affect the fetal-maternal environment warrants further exploration. This study compared endocrine, fetal, placental, and uterine parameters during early pregnancy (through day 70) in mares with elevated leptin versus normal leptin (NL) concentrations. Fourteen mares were classified as either NL (n = 6; Lep 0.9 ± 0.3 ng/mL), moderate leptin (ML; n = 4; Lep 3.0 ± 0.3 ng/mL), or high leptin (HL; n = 4; Lep 5.8 ± 0.7 ng/mL) based on annual plasma leptin. Mares were artificially inseminated and induced …

Dose Characterization Of Galactic Cosmic Rays In The Human Brain: A Phits-Based Study With A Segmented Anatomical Phantom, Shanice N. Manning

LSU Master's Theses

Astronauts aboard the International Space Station (ISS) are chronically exposed to a complex space radiation environment composed of galactic cosmic rays (GCR), solar particles, and Earth's trapped radiation. These high-energy particles interact with spacecraft shielding, producing secondary particles that can penetrate inside and deposit energy in sensitive tissues. Although animal studies have been useful in assessing space radiation risks, anatomical and physiological differences limit their applicability to human anatomy especially neuroanatomy. To address this gap, we developed a computational framework to model radiation dose distribution within the human brain using Monte Carlo simulations in Particle and Heavy Ion Transport code …

Anxiety's Impact On Autism Spectrum Disorder Symtomology: An Investigation Into The Relationship Between Two Comorbities, Michaela Brown

LSU Doctoral Dissertations

Autism spectrum disorder (ASD) has been found to be frequently comorbid with many medical and psychological disorders, one of which is anxiety disorders. Research has shown that, when anxiety is present within ASD, various areas of the child’s functioning may be impacted, such as ASD symptom severity, emotional regulation skills, adaptability, social skills and daily living skills. The current study aimed to investigate this relationship of anxiety and ASD. First, within a sample of children and adolescents with ASD, the magnitude of the correlation between specific ASD symptoms parent-reported anxiety was compared to determine if certain ASD symptoms were more …

Gift Funds Two Graduate Researchers' Summer Projects, Clara Turnage

University of Mississippi News

OXFORD, Miss. – Two University of Mississippi graduate students got to pursue their research goals this summer thanks to a gift from alumna Frances Permenter Smith, a longtime friend of the university.

Global Scale Assessment Of The Human-Induced Extinction Crisis Of Terrestrial Carnivores., Erik Joaquín Torres-Romero, Timothy M. Eppley, William J. Ripple, Thomas M. Newsome, Miha Krofel, Neil H. Carter, Andrés Ordiz, Tadeu G. De Oliveira, Nuria Selva, Vincenzo Penteriani

Anthropology Faculty Publications and Presentations

Human impacts on carnivores are a persistent conservation challenge worldwide. We present a global analysis showing the overlap of conservation lands and the cumulative impact of humans on the distribution ranges of 257 terrestrial carnivore species. Our findings reveal that 64% of carnivore ranges overlap with areas characterized by high human pressures. We found that Indigenous peoples' lands emerge as crucial for carnivore population viability, potentially safeguarding 26% of carnivore ranges globally, while protected and wilderness areas cover roughly 10 and 16% of carnivore ranges, respectively. These three areas combined cover 35% of the global range of carnivores. Reducing human …

Hyperspectral Band Selection Via Heterogeneous Graph Convolutional Self-Representation Network, Junde Chen, Wenzhao Li, Surendra Maharjan, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Hyperspectral image (HSI) band selection (BS) plays a crucial role in HSI dimensionality reduction, aiming to identify a representative subset of bands with minimal redundancy. However, conventional BS approaches primarily operate in the Euclidean domain, often overlooking the structural characteristics of pixels and spectral bands, such as spatial continuity and spectral dependencies. In addition, they handle each HSI as an integrated unit to harness implicit spatial information, disregarding spatial distribution variations across different homogeneous regions. To fully leverage structural information, this study introduces a novel BS method, termed the dual heterogeneous graph convolutional network with enhanced self-representation (ESR-HGCN), for HSI …

Childhood Skeletal Lesions Common In Prehistory Are Present In Living Forager-Farmers And Predict Adult Markers Of Immune Function, Amy S. Anderson, Aaron Blackwell, M. Linda Sutherland, Thomas Kraft, James Sutherland, Bret Beheim, Daniel K. Cummings, Suhail Ghafoor, Paul L. Hooper, Daniel Eid Rodriguez, Andrei Irimia, Margaret Gatz, Wendy Mack, Chris Rowan, Michael Miyamoto, Kenneth Buetow, Caleb Finch, L. Samuel Wann, Adel Allam, Randall C. Thompson, Gregory Thomas, Hillard Kaplan, Jonathan Stieglitz, Benjamin Trumble, Michael D. Gurven

ESI Publications

Porous cranial lesions (cribra cranii and cribra orbitalia) are widely used by archaeologists as skeletal markers of poor child health. However, their use has not been validated with systematic data from contemporary populations, where there has been little evidence of these lesions or their health relevance. Using 375 in vivo computed tomography scans from a cohort-representative sample of adults aged 40+ years from the Bolivian Amazon, among food-limited, high-mortality forager-farmers, we identified cribra cranii on 46 (12.3%) and cribra orbitalia on 23 (6%). Cribra orbitalia was associated with several hallmarks of compromised immune function, including fewer B cells, fewer naïve …

Impacts Of Artificial Habitat Structures On Overwinter Fish Survival And Fish Presence In South-Central Nebraska, Logan Dietrich

Biology Theses, Dissertations, and Student Creative Activity

Artificial lentic systems, such as borrow pits, often lack structural complexity due to construction practices and steep bathymetry, limiting features like vegetation and woody debris essential for fish communities. To address this, artificial structures made of materials such as polyvinyl chloride are increasingly used by state and federal agencies to supplement habitat. However, little information is known about the impact these structures have on fish communities. Thus, the objectives of this study were to examine the influence of Georgia cubes in borrow pit impoundments on overwinter survival of sub-stock and ≥ stock Bluegill (Lepomis macrochirus) and Largemouth Bass …

Applications Of Eco-Friendly Synthesis Of Copper Oxide Nanoparticles In Manufacturing Of Sensors For Potentiometric Determination Of Tetracycline Hydrochloride, Amina M. Abass, Fadam M. Abdoon

Baghdad Science Journal

A new type of electrodes was prepared for determination of drugs depended on (TCH- PM-CuO NPs). This work recommended a novel CuO nanoparticles-depended on coated wire sensors for detected tetracycline hydrochloride (TCH) in pure and pharmaceutical formulations. Tetracycline, hydrochloride, and molybdophosphoric acid (PM) were combined in the finding of polymeric poly vinyl chloride (PVC) and o -nitro phenyl octyl ether (o -NPOE) as a solvent mediator to formula an electro-active material is tetracycline- molybdophosphoric acid (TCH-PM). The adapted sensors utilizing TCH-PM-CuO nanoparticles showed high selectivity and sensitivity for the quantification and discrimination of TCH with a linear range 1.0 × …

Synthesis Of Salicylaldehyde Derivatives And Evaluating Its Antimicrobial, Antifungal, And Anticorrosion Activities, Ibrahim Mamedov, Ali Khalilov

Baghdad Science Journal

Chalcones are natural, unsaturated aromatic ketones used in organic synthesis and medicine. Nevertheless, obtaining synthetic analogs and studying their reactions remains relevant. Unsaturated aromatic ketones are important substrates for synthesizing various heterocyclic compounds. The new chalcone, dihydropyrazole derivatives were synthesized based on 2-hydroxy-5-phenyldiazenyl benzaldehyde, and their structures were studied by NMR. Many metal structures fail each year due to harsh conditions, causing significant damage to the global economy. To overcome this problem, chemists are constantly researching and developing new corrosion inhibitors. The compounds under test contain nitrogen, oxygen, and sulfur atoms, which can be coordinated with metals. These types of …

Synthesis, Characterization, And An Evaluation Of The Biological Activities Of Some New Transition Metal Complexes With Organic Ligand, Ghadeer Alataby, Areej Kamal Assim Aldabbagh

Baghdad Science Journal

The reaction between triethylorthoformate and p-chlorophenyl resulted in formation of N,N'-bis-(p-chlorophenyl)formamidine ligand. Subsequently, this ligand interacted with the following metal ions (Co⁺², Ni⁺², Cu⁺², Cr⁺³ and Ru⁺³) creating air-stable complexes with formula [M(L)₂(H₂O)_n(Cl)_x](X= 0, 1) with distinct octahedral morphologies. The formation of these compounds was identified using mass spectroscopy (MS), which validated the acquired geometries. Fourier transform infrared (FT-IR) spectroscopy analysis demonstrated how the formamidine group formed and how it coordinated with the metal ions. Studies using pyrolysis (Thermogravimetric analysis (TGA) …

Elimination Of Hiv Reservoirs Harboring Intact Proviruses, Min Li, Khanghy Truong, Suman Sharma, Baichao Sun, Min Chen, Jason T Kimata, Jin Wang

Faculty, Staff and Students Publications

Background: Human immunodeficiency virus (HIV) establishes persistent infection by integrating its proviral DNA into the host genome. While most integrated proviruses are defective, a small portion of proviruses are intact that represent a major obstacle to achieving an HIV cure. We have designed an approach for selective elimination of host cells harboring replication-competent HIV (SECH), through inhibition of autophagy and anti-apoptotic molecules during viral reactivation. However, the effects of SECH on the dynamics of intact and defective HIV provirus remain unclear.

Methods: We isolated DNA from HIV-infected samples after SECH treatments. We analyzed HIV-1 proviruses in these samples by intact …

Spase: Spatially Resolved Pathology Scores Using Optimal Transport On Spatial Transcriptomics Data, Mohammad Nuwaisir Rahman, Mohammed Abid Abrar, Vikram Rakesh Shaw, James F Martin, M Saifur Rahman, Md Abul Hassan Samee

Faculty, Staff and Students Publications

Pathological events often impact tissue regions in a spatial variable manner, making it challenging to identify therapeutic targets. Spatial transcriptomics (ST) is a powerful technology to map spatially variable molecular mechanisms, yet suitable analytical methods have been lacking. We introduce SPaSE (Spatially-resolved Pathology ScorE), an optimal transport-based algorithm to compare ST data from diseased and control tissues. SPaSE computes a “pathology score” for each spot in the diseased sample, quantifying the pathological impact at that spot. In post-MI (myocardial infarction) mouse hearts, these scores delineated zones that matched independent expert annotations. Modeling pathology scores from gene expression revealed signatures predictive …

Development And Extensive Sequencing Of A Broadly-Consented Genome In A Bottle Matched Tumor-Normal Pair, Jennifer H Mcdaniel, Vaidehi Patel, Nathan D Olson, Hua-Jun He, Zhiyong He, Kenneth D Cole, Alexander A Gooden, Anthony Schmitt, Kristin Sikkink, Fritz J Sedlazeck, Harsha Doddapaneni, Shalini N Jhangiani, Donna M Muzny, Marie-Claude Gingras, Heer Mehta, Sairam Behera, Luis F Paulin, Alex R Hastie, Hung-Chun Yu, Victor Weigman, Alison Rojas, Katie Kennedy, Jamie Remington, Isai Salas-González, Mitch Sudkamp, Kelly Wiseman, Bryan R Lajoie, Shawn Levy, Miten Jain, Stuart Akeson, Giuseppe Narzisi, Zoe Steinsnyder, Catherine Reeves, Jennifer Shelton, Sarah B Kingan, Christine Lambert, Primo Baybayan, Aaron M Wenger, Ian J Mclaughlin, Aaron Adamson, Christopher Kingsley, Melanie Wescott, Young Kim, Benedict Paten, Jimin Park, Ivo Violich, Karen H Miga, Joshua Gardner, Brandy Mcnulty, Gail L Rosen, Rajiv Mccoy, Francesco Brundu, Erfan Sayyari, Konrad Scheffler, Sean Truong, Severine Catreux, Lesley Chapman Hannah, Doron Lipson, Hila Benjamin, Nika Iremadze, Ilya Soifer, Gat Krieger, Stephen Eacker, Mary Wood, Erin Cross, Greg Husar, Stephen Gross, Michael Vernich, Mikhail Kolmogorov, Tanveer Ahmad, Ayse G Keskus, Asher Bryant, Francoise Thibaud-Nissen, Jonathan Trow, Jacqueline Proszynski, Jeremy Wain Hirschberg, Krista Ryon, Christopher E Mason, Mital S Bhakta, J Zachary Sanborn, Elizabeth M Munding, Justin Wagner, Chunlin Xiao, Andrew S Liss, Justin M Zook

Faculty, Staff and Students Publications

The Genome in a Bottle Consortium (GIAB), hosted by the National Institute of Standards and Technology (NIST), is developing new matched tumor-normal samples, the first explicitly consented for public dissemination of genomic data and cell lines. Here, we describe a comprehensive genomic dataset from the first individual, HG008, including DNA from an adherent, epithelial-like pancreatic ductal adenocarcinoma (PDAC) tumor cell line and matched normal cells from duodenal and pancreatic tissues. Data for the tumor-normal matched samples comes from seventeen distinct state-of-the-art whole genome measurement technologies, including high depth short and long-read bulk whole genome sequencing (WGS), single cell WGS, Hi-C, …

Spectrophotometric Determination Of Bromhexine-Hcl By Using Prepared Silver Nanoparticles, Zainab Noori, Muna M. Khudhair

Baghdad Science Journal

In this study, a new, simple, and rapid method was developed for determining Bromhexine-HCl using silver nanoparticles. The solution of silver nanoparticles has a yellow color which has absorbency at a wavelength of 404 nm. Bromhexine was estimated through its reaction with silver nanoparticles in acidic environment at pH 5. This reaction causes the silver nanoparticles to aggregate and change the color of their colloidal solution from yellow to brown, leading to a decrease in their absorbance. The range of linearity was found to be 20-250μg mL-1, a limit of detection is equal to 18.931μg/mL and limit of quantitation is …

Advancing Eye-Gaze Writing Systems With Computer Vision, And Dynamic Text Suggestions, Walid Abdallah Shobaki

Theses and Dissertations

Eye gaze writing, a novel interaction modality, has the potential to revolutionize communication for individuals with limited mobility. In our research, we investigated the deep learning algorithms efficiency for real-time eye gaze writing. We have compared many algorithms' performance in many computer vision areas, such as object detection in which we used first YOLOv8, the second algorithm SSD, and the third algorithm is Faster R-CNN, the second computer vision area is the image segmentation in which we used DeepLab and U-Net, and the last computer vision area is self-supervised learning we have used SimCLR algorithm. By evaluating these models on …

Role And Electrophysiological Properties Of Sexually Dimorphic Oxytocin Receptor Expressing Neurons In The Anteroventral Periventricular Nucleus Of The Female Mice, Armita Abdollahi Govar

LSU Doctoral Dissertations

Oxytocin is a neurohypophyseal hormone that plays a crucial role in regulating mother-infant bonding and maternal behavior. Oxytocin modulates sex-specific parental behaviors by binding to the oxytocin receptor (OXTR)-expressing neurons in the brain. This study investigates the sexually dimorphic OXTR neurons in the anteroventral periventricular nucleus (AVPV) to understand how oxytocin modulates behavior in females. Specifically, we explored the behavioral roles, neural projections, and intrinsic electrophysiological characteristics of these neurons in the female mice. Using immunohistochemistry revealed that the number of OXTR-expressing neurons was enhanced in postpartum females compared with virgin females, suggesting that these neurons are involved in female-specific …

Specialty Grand Challenge: Existing Issues And Emerging Frontiers In Renal Endocrinology, A. T. M. Emdadul Haque, Mohammed S. Razzaque

School of Medicine Publications

We are currently in an exciting time to advance the field of renal endocrinology. There has never been a more favorable time to study the molecular and cellular mechanisms underlying renal hormone functions in physiology, pharmacology, and pathophysiology. The availability of advanced research tools provides unique opportunities to dissect the complex regulatory roles of renal hormones. The kidneys are vital organs in the human body; each one is composed of approximately one million functional nephrons. Understanding the precise roles and regulation of kidney-derived hormones and hormones that exert endocrine effects on the kidneys will help in preventing or delaying various …

Altered Patterning Of Neural Activity In A Neuropathology, Clarissa Hoffman, Jingheng Cheng, Rodrigo Morales, Daoyun Ji, Yuri Dabaghian

Faculty, Staff and Students Publications

The dynamics of neural circuits and their role in mediating cellular and organismal phenomena remain poorly understood, despite numerous efforts to dissect these processes through precise instantaneous measurements or longer-time averages and approximations. We use an alternative approach: we investigate these dynamics at the system's mesoscale by analyzing spike trains and waveforms. These extended activity patterns carry robust, tractable information, are highly responsive to physiological specifics, and enable detailed tracking of circuit behavior. In particular, this methodology allows for characterizing the functionality of tau-pathology-afflicted hippocampal circuits and identifying circuit-level abnormalities that are missed by through traditional analyses. In healthy mice, …

Research And Education In Robotics: A Comprehensive Review, Trends, Challenges, And Future Directions, Mutaz Ryalat, Natheer Almtireen, Ghaith Al-Refai, Hisham Elmoaqet, Nathir Rawashdeh

Michigan Tech Publications

Robotics has emerged as a transformative discipline at the intersection of the engineering, computer science, and cognitive sciences. This state-of-the-art review explores the current trends, methodologies, and challenges in both robotics research and education. This paper presents a comprehensive review of the evolution of robotics, tracing its development from early automation to intelligent, autonomous systems. Key enabling technologies, such as Artificial Intelligence (AI), soft robotics, the Internet of Things (IoT), and swarm intelligence, are examined along with real-world applications in healthcare, manufacturing, agriculture, and sustainable smart cities. A central focus is placed on robotics education, where hands-on, interdisciplinary learning is …

Human Development, Inequality, And Their Associations With Brain Structure Across 29 Countries, Vicente Medel, Luz M. Alliende, Richard Bethlehem, Jakob Seidlitz, Grace Ringlein, Celso Arango, Aurina Arnatkevičiūtė, Laila Asmal, Mark Bellgrove, Anderson M. Winkler

Human Genetics Publications

Background: The macro-social and environmental conditions in which people live, such as the level of a country’s development or inequality, are associated with brain-related disorders. However, the relationship between these systemic environmental factors and the brain remains unclear. We here aimed to determine the association between the level of development and inequality of a country and the brain structure of healthy adults.

Methods: We conducted a cross-sectional study pooling brain imaging (T1-based) data from 145 magnetic resonance imaging (MRI) studies in 7,962 healthy adults (4,110 women) in 29 different countries. We used a meta-regression approach to relate the brain structure …

Assessment Of Decorin Level And Some Biochemical Parameters In Iraqi Patients With Diabetic Nephropathy, Zahraa M. Ismail, Falah S. Al-Fartusie, Ahmed H. Ismail, Noor T. Tahir

Baghdad Science Journal

Diabetic nephropathy (DN) is a serious kidney complication that can occur in individuals with diabetes; around 20% to 30% of individuals with Type 2 diabetes (T2D) develop diabetic nephropathy (DN), which is a persistent microvascular complication. The DN is often considered the leading contributor to end-stage renal failure. This study aimed to assess decorin levels and their correlation with various biochemical variables among three different groups: 40 patients with T2DM nephropathy (G1), 40 patients with T2DM without nephropathy (G2), and a healthy control group (G3). The study included a total of 120 participants, divided into three groups T2DM with DN …

Fabrication Of Dye-Sensitized Solar Cell Using Cuo Nanoparticles As A Photo Anode, Ahmed Mahdi Rheima, Athra G. Sager, Mahdi A. Mohammed, Ali Jafer Mahdi

Baghdad Science Journal

Nanoparticles of copper oxide (CuO) are synthesized using a photolysis process. x -ray diffraction (XRD]) is utilized to investigate CuO nanoparticle's crystal structure, and transmission electron microscopy (TEM) is employed to analyze the morphology and the average nanoparticle diameters. Results indicated that the CuO nanoparticles have a monoclinic shape with a mean size of 14 nm. The UV -- Visible measurement is shown that the bandgap of CuO nanoparticles is 1.61 eV. The CuO nanoparticles were applied in a photoanode section to fabricate photo organic sensitizer solar cells. The 4,4'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(azaneylylidene))bis (methaneylylidene)) bis (2-methoxy-phenol) was synthesized via the Microwave irradiation method …

Studying The Level Of Carboxy-Terminal Cross Link Telo Peptide Type-1 Collagen And Human Cartilage Glycoprotein In Sera Of Iraqi Patients With Knee Osteoarthritis, Asmaa Rafea Mohamed, Bushra F. Hasan

Baghdad Science Journal

Knee osteoarthritis (KOA), commonly known as degenerative joint disease, is frequently brought on by wear-and-tear articular cartilage loss that occurs over time and most often affects the elderly. Since cartilage is the main element that breaks down when OA occurs, its chemical components can be exploited to develop a sensitive biomarker to identify the beginning and progression of OA disease. The study aimed to evaluate and assess the alteration in the serum level of collagen type I C-telopeptide (CTX-1), and human cartilage glycoprotein (YKL-40) in serum samples obtained from (81) patients diagnosed with Knee Osteoarthritis who were hospitalized in Baghdad …

Adsorption Of Pb(Ii), Cu(Ii), And Ni(Ii) By A New Magnetic Schiff Base Resin: Synthesis, Characterization, And Thermal Study, Iman Saad Ali

Baghdad Science Journal

This study dealt with the preparation and analytical study of a type of chelating resin. This type of resin was distinguished by containing a ligand of the type of aromatic Schiff bases (5A-T) by reacting the aromatic amine (5-aminoisopthalic acid) with terephthaldehyde. The prepared Schiff base was identified using infrared FT-IR, 1H-NMR, 13C-NMR, and precise element analysis technology. The prepared Schiff bases were converted into azomethine-amide resin P5A-T by reacting the ligand (5A-T) with the diamines (4, 4-oxydianiline). The prepared resin was characterized using infrared spectroscopy. The study also included the preparation of magnetic zinc iron oxide (ZnFe2O4) by covalent …

Cryo-Em Structures Of A Pentameric Ligand-Gated Ion Channel In Liposomes, Vikram Dalal, Brandon K Tan, Hanrui Xu, Wayland W L Cheng

2020-Current year OA Pubs

Detergents and lipid nanodiscs affect the cryo-EM structures of pentameric ligand-gated ion channels (pLGICs) including ELIC. To determine the structure of a pLGIC in a membrane environment that supports ion channel function, we performed single particle cryo-EM of ELIC in liposomes. ELIC activation and desensitization were confirmed in liposomes with a stopped-flow thallium flux assay. Using WT ELIC and a non-desensitizing mutant (ELIC5), we captured resting, activated, and desensitized structures at high resolution. In the desensitized structure, the ion conduction pore has a constriction at the 9' leucine of the pore-lining M2 helix, indicating that 9' is the desensitization gate …