Digital Commons Network^™

From Misinformation To Policy Solutions: Teaching Herd Immunity And Vaccine Hesitancy Through Real-World Case Studies Of Measles Outbreaks, Laurieann Klockow

Biomedical Sciences Faculty Research and Publications

Vaccine hesitancy threatens herd immunity, contributing to the resurgence of measles outbreaks. This lesson engages upper-level biomedical science students in analyzing the complex interplay between vaccination rates, herd immunity and vaccine exemption policies through examination of the 2019 New York measles outbreak that threatened the United States’ measles elimination status. Students critically evaluate anti-vaccine misinformation, analyze epidemiological data and develop evidence-based policy recommendations to increase vaccination rates. The lesson combines team-based learning with authentic assessment through vaccine policy development, moving beyond knowledge acquisition and content to building scientific literacy and evidence-based reasoning skills essential for scientifically literate citizens and future …

Using Rna Sequencing To Examine Encephalic Gene Expression In Cactus Mice, Sarah A. Nicholls

Honors Theses and Capstones

Many of the exact mechanisms by which desert-adapted animals avoid dehydration-induced complications are unknown, though there are likely ties to the general mammalian dehydration response, a multisystem process spearheaded by the brain. Peromyscus eremicus, the cactus mouse, can survive its whole life without intaking water and is commonly used as a model to study desert adaptations. In this study, we have used bulk transcriptomics to characterize the differential gene expression across hydrated and dehydrated P. eremicus in five major regions of the brain: the cerebral cortex (referred to throughout much of this paper as the cortex), hippocampus, thalamus, midbrain, …

Investigating The Dna-Binding Role Of Tbp1 In Toxoplasma Gondii: Expression And Characterization Of A Recombinant Protein, Madison T. Jeckell

Honors Theses and Capstones

Toxoplasma gondii is an obligate intracellular parasite with a complex life cycle and an unusual transcriptional machinery compared to other eukaryotes. Unlike most organisms, Toxoplasma lacks multiple well-conserved promoter motifs and has multiple TATA-binding proteins (TBPs), including TBP1, whose function in DNA binding and transcription regulation is poorly understood. This study aimed to generate and express a recombinant TBP1 protein from Toxoplasma to enable future DNA-binding assays and motif identification. Using RT-PCR, the TgTBP1 gene was amplified and cloned into a pGEX vector to produce a GST-tagged protein. The recombinant plasmid was transformed into E. coli BL21 cells, and protein …

Reasoning Through Change: Exploring Covariational Thinking In Student Exponential Graph Interpretation, Samantha Lynn Pearson

Honors Theses and Capstones

This study explores how life science students reason about exponential functions in the context of an introductory physics course. While exponential functions are essential in both physics and biology—appearing in scenarios like population growth and temperature cooling—students often encounter challenges in interpreting them. Using a resources framework, we examined the cognitive resources these students bring to exponential reasoning, focusing on what ideas and strategies they already possess. Our methodology involved conducting think-aloud interviews with nine students from PHYS 402. Interviews were coded using a framework informed by prior research on covariational reasoning and exponential understanding. Results show that students generally …

Waking The Witch: Exploring Gender, Magic, And Power Dynamics In Ancient Rome, Diana Gibson

Honors Theses and Capstones

This thesis explores the presence of witches and magic in the literature of the Roman Empire. The study ranges from the 1st century BCE to the 6th century CE, and relates the prevalence of female witches in the Roman literature to particularly turbulent social contexts, such as the fall of the Republic and the rise of Christianity. The piece considers the rhetorical nature of accusations of witchcraft, and how the concepts of magic and witchcraft themselves were defined and shaped by those in power to uphold imperial hegemony and structures of patriarchy. The project seeks to highlight a distinction between …

Optimization Of Small-Scale Bioreactors For Increased Pdna Production And Yield, Laura M. Hills

Honors Theses and Capstones

No abstract provided.

18s Metabarcode Analyses Of Eukaryotic Species In The Respiratory Microbiomes Of Wild Canids From New Hampshire, Collin Sinclair Blake

Honors Theses and Capstones

This study is of an exploratory nature and focuses on characterizing the eukaryotic microbiota present in the respiratory tissues of six wild canids and one domestic canine. The contents of this document largely pertain to dry lab analyses of 18S barcodes in bioinformatics programs – primarily QIIME2 running in the GitBash command line, service for which was hosted by the UNH Ron Bioinformatics training server. All procedures listed within the section below were performed by second parties at the UNH Hubbard Center for Genomics Studies (HCGS), the New Hampshire Veterinary Diagnostics Lab (NHVDL), and the UNH Microbial Ecology and Emerging …

Production & Perception Of /S/ And Pitch Of Transgender Speakers, Connor J. Ryan

Honors Theses and Capstones

Linguistic research on how transgender speakers use speech traits commonly associated with gender, such as f0 and /s/, is still limited in scope. Past research has focused primarily on singular gender groups within the transgender community, particularly transmasculine speakers, leaving gaps in how speech differs among transgender speakers. Additionally, there has been little research on how transgender identity influences perception of gender in speech. Here, I use data from reading passages by fifteen university-age transgender speakers in New England to analyze variation in /s/ and f0 among transmasculine, transfeminine, nonbinary, and multigender speakers. I then performed a perception study using …

Increased Visual Response Delay Impact On Sensorimotor Control In Persons With Multiple Sclerosis, Julie C. Wagner, Frankie M. Ingram, Vincenzo Daniele Boccia, Matilde Inglese, Maura Casadio, Camilla Pierella, Andrea Canessa, Robert A. Scheidt, Scott A. Beardsley

Biomedical Engineering Faculty Research and Publications

Multiple Sclerosis negatively affects hand function in 60% of cases. Upper extremity dysfunction in persons with Multiple Sclerosis (PwMS) has previously been linked to slower, more variable movement, increased visual response delays (T_v), and neuroimaging evidence of altered brain activity; yet no one knows how these aspects relate to each other. This work combines clinical, kinematic, sensorimotor control, and neural imaging techniques to gain a more complete understanding of how upper extremity dysfunction arises in PwMS. Twenty PwMS and 20 Controls completed a reach and hold task with simultaneous electroencephalography recorded to determine if increased T_v in …

Examining Exercise Self- Efficacy And Functional Mobility In Urban Law Enforcement Officers, Margo F. Jenkins

College of Graduate Studies: Theses & Dissertations

BACKGROUND: Law enforcement officers perform various physically demanding tasks, but much of their daily activities are sedentary or light intensity. Quality movement patterns are vital for fitness, reducing the risk of injuries, and contributing to motivation for physical activity. PURPOSE: This study examined the relationship between exercise self-efficacy (ESES) and functional mobility (FMS) among law enforcement officers. METHODS: 55 officers participated, with exercise self-efficacy measured using the ESES scale and movement patterns assessed with the Functional Movement Screening (FMS). Correlational analysis and linear regression were used to explore the relationships between FMS and ESES, years of service (YoS) and FMS, …

Investigating Concealments In Infanticide: Incidents And Forensic Entomological Implications, Gillian L. Johnson

College of Graduate Studies: Theses & Dissertations

Forensic entomology plays a critical role in estimating the minimum postmortem interval (mPMIs), based on insect evidence. When victims are concealed, this can complicate mPMI estimates. Utilizing data from the National Violent Death Reporting System (NVDRS) from 2018 to 2021, I found that victims of infanticides and neonaticides are often concealed. I first investigated the demographics of offenders and victims, how often victims are concealed, and the most common means of concealment. I found that victims of infanticide were likely to be concealed 5% of the time, and neonaticides 50% of the time. Next, I investigated how concealment alters insect …

Variations Of Hex Bar Deadlifts On Post-Activation Performance Enhancement In Collegiate Baseball Athletes, Samuel Sanders

College of Graduate Studies: Theses & Dissertations

Single-session resistance priming protocols are used by strength and conditioning coaches to acutely improve neuromuscular mechanisms that are beneficial to athletic performance. This study aimed to examine if a concentric-only hex bar deadlift (CHBDL) would produce a similar post-activation performance enhancement (PAPE) effect as a full-range-of-motion hex bar deadlift (THBDL) and mitigate perceived measures of fatigue and soreness in collegiate athletes. 19 NCAA Division I baseball athletes completed the study. A repeated measures design with randomized assignment and counterbalanced order was utilized. Over two weeks, the CHBDL and THBDL were added into a typical priming protocol used by the team’s …

Effects Of Perinatal Exposure To Lipopolysaccharide And Acetaminophen On Learning In Adult Long-Evans Rats, Lucy A. Sivils

College of Graduate Studies: Theses & Dissertations

The present study investigated the individual and combined effects of elevated perinatal inflammation, oxidative stress caused by acetaminophen (APAP) exposure, or elevated inflammation and oxidative stress on adult learning behaviors in Long-Evans rats. While prior studies have examined these factors independently, the combined effects of inflammation and oxidative stress via APAP exposure during early development on learning-related behaviors remain unexplored. The present research aimed to address this gap by the analysis of potential long-term neurodevelopmental consequences of lipopolysaccharide (LPS)-induced inflammation and APAP-induced oxidative stress. LPS, APAP, or LPS + APAP was administered during the perinatal period: postnatal days (PD) 9, …

Synthesis Of Tu100 Analogs And Biological Assays, Feyisope Tonye Oladapo

College of Graduate Studies: Theses & Dissertations

TU100 (14-methyl-5H-5,12-epiminobenzo[4,5]cyclohepta[1,2-b]naphthalene-6,11,13(12H)-trione) exhibits exceptional chemotherapeutic properties. It operates via a distinct mechanism that triggers cell death to create cytotoxic effects while concurrently inhibiting topoisomerase I and II. Its efficacy rivals that of well-known chemotherapeutic drugs like Daunorubicin. The distinctive characteristics of TU100 have led to initiatives aimed at creating structurally similar compounds to discover even more effective bioactive alternatives.

The analog synthesis incorporated quinoxaline or its derivatives (diphenyl quinoxaline, dipyridinyl quinoxaline, and dibenzophenazine-10,13-dione) that have been shown to have promising chemotherapeutic effects. Synthesizing the hetero naphthoquinones required a multi-step synthetic process, so quinoxaline/ derivatives intermediates are first prepared, then the …

A Feminist Narrative Shaped By Bangladesh’S Threads, Salma Akter

College of Graduate Studies: Theses & Dissertations

This thesis explores the intersection of feminist theory and artistic practice, examining how materiality and abstraction reflect gender norms within the socio-political, religious, and colonial context of Bangladesh. It is grounded in four key theoretical frameworks: judith butler’s gender performativity, bell hooks’ intersectional feminism, julia kristeva’s abjection and systems theory. Together, these frameworks provide a comprehensive lens for analyzing how gender inequality is constructed, maintained, and resisted within the socio-political, religious, and colonial context of Bangladesh. Textile-based media serve as a means to engage with these layered systems, drawing from traditional nakshi kantha embroidery to explore patterns of labor, domesticity, …

Survey And Identification Of Entomopathogenic Fungi In Millipede Species In Coastal Georgia, Kristin L. Campbell Bailey

College of Graduate Studies: Theses & Dissertations

Entomopathogenic fungi are well-known, species-specific pathogens of a wide diversity of insects that infect, manipulate, and kill their insect hosts to complete their life cycle. Recently in Savannah, Georgia, millipedes belonging to the genus Eurymerodesmus were observed exhibiting pre-death climbing behaviors associated with pathogenic fungal infection. Dying millipedes summited vegetation and subsequently died in elevated positions along edge habitat transitioning a recreational field and forest. Upon inspection, fungal structures found emerging from dead millipedes anatomically resembled Arthrophaga myriapodina, which is the first documented entomophthoralean fungus to infect millipedes. The goal of this research was to obtain species-level identification of the …

Quantifying Inositol Phosphate Dephosphorylation To Understand The Role Of Recalcitrant Organic Phosphorus Forms On Harmful Algal Blooms In Freshwater Systems, Iffat Tasnim

College of Graduate Studies: Theses & Dissertations

Organophosphorus such as phytic acid, a surrogate species of inositol phosphate (IP), may serve as a source for orthophosphate (OP) in freshwater. In absence of OP, competent aquatic microorganisms upregulate the production of specialized enzymes to obtain growth-sustaining OP from organic phosphorus (org-P) forms. The contribution of recalcitrant org-P to the OP pool has been overlooked due to the lack of capable tools to measure OP production from org-P accurately. The objective of this study was to quantify OP production from phytic acid (a surrogate form of recalcitrant org-P) to assess the contribution of recalcitrant org-P forms to the total …

A Finite Volume-Based Approach For Modeling Hyperbaric Oxygen Therapy For Healing Chronic Wounds, Cade M. Layser

College of Graduate Studies: Theses & Dissertations

The current number of people in America with diabetes is approximately 38.4 million, that population is expected to rise to 54.9 million by 2030. Chronic wounds, which are characterized by impaired or stalled healing, are commonly associated with diabetes. These wounds if not treated properly can cause significant complications such as sepsis, infection, and gangrene which can lead to limb amputation or death of the patient. Limb amputations can be a large financial burden and significantly reduce the patient’s quality of life. The timely and efficient treatment of chronic wounds is essential to prevent adverse patient outcomes. Hyperbaric Oxygen therapy …

Defect Detection In Extrusion-Based Bio-Printed Constructs Using Video-Based Vibration Analysis, Md Anisur Rahman

College of Graduate Studies: Theses & Dissertations

Bio-additive manufacturing has emerged as a transformative advancement in biomedical engineering, enabling the fabrication of complex biological structures. However, maintaining the precision and quality of printed constructs remains a significant challenge, particularly in applications such as bioprinted organ transplants. Among the key factors affecting structural integrity, dimensional inconsistencies and interlayer defects present major obstacles, potentially compromising mechanical stability and functionality. This study explores the detection of defects caused by variations in dimensional accuracy and layer-to-layer adhesion. To address these challenges, a video-based motion estimation technique is employed as a non-contact and non-destructive evaluation method. Traditional approaches, such as contact-based and …

Exploring Coastal Plain Fish Ecology Across A Range Of Early-Life-History Traits And River Types, Bridgette Nicolosi

College of Graduate Studies: Theses & Dissertations

The ecological complexity of southeastern Coastal Plain rivers is reflected in the diversity of fish communities they support, yet the processes structuring early life dynamics remain poorly understood. This thesis explores the influence of environmental gradients on larval fish assemblage composition and American Shad (Alosa sapidissima) spawning phenology across two Georgia Coastal Plain river systems.

In the unregulated Ogeechee River, I investigated spatial and temporal variation in larval fish communities by comparing mainstem and tributary habitats to understand the role of tributaries in watershed-wide larval production. Larvae were collected biweekly from May to September 2024 at eight paired mainstem-tributary sites …

Long-Term Ichthyoplankton Monitoring Of The Moon River (Savannah, Ga) With A Focus On Climate Change, Bradley Cayson

College of Graduate Studies: Theses & Dissertations

The presence of healthy fish populations is a cornerstone of many important ecological mechanisms. These populations are supported by the recruitment of ichthyoplankton, or the egg and larval stages of fishes, as the primary determinant of population growth. Increases in average global water temperatures can alter the composition of fish communities and thus the offspring found in planktonic stages. I compared a large data set of ichthyoplankton surveys completed from 2009-2011 to a replicated six-month sampling period from May – November of 2024. Collection was conducted weekly to biweekly during night flood tides using a fixed suspension ichthyoplankton net (1mm …

A Phenomenological Study Exploring The Lived Experiences Of First-Generation African American Students To Improve Retention Rates, Jaleesa Porter

College of Graduate Studies: Theses & Dissertations

Colleges and universities play an important role in maintaining high retention rates and getting students from matriculation to graduation. There are several studies related to the reasons and barriers that result in low retention rates. This qualitative study examined how one university in southeast Georgia can improve retention rates among first-generation African American students by enhancing its practices. The findings from this study the revealed lived experiences of college students that resulted in a range of emotions, resources and barriers, and communication. Discussion and implications of the findings provide recommendations for practice and future research.

Bringing A Missing Minority Into Focus: Foster Parents’ Voices And Their Impact On Foster Children’S Academic Experiences, Clair Rutherford

College of Graduate Studies: Theses & Dissertations

Foster children face persistent educational inequities shaped by trauma, frequent placement changes, and systemic barrierswithin schools. This dissertation investigates how foster parents perceive and influence the educational experiences of the children in their care, emphasizing the intersection of advocacy, trauma-informed teaching, and institutional response. Grounded in trauma-informed pedagogy, critical ecology and curriculum studies, the study interrogates how schools both support and constrain foster children’s educational opportunities. An arts-based methodology was employed, combining fictionalized portraiture and digital ethnography. Data sources included foster parent social media posts, reflexive journalling, and researcher positionality as both teacher and foster parent. Composite narratives were constructed …

The Role Of Heterotrophy In The Response Of Oculina Arbuscula To Ocean Acidification, Alejandra E. Daniel

College of Graduate Studies: Theses & Dissertations

On both tropical and temperate reefs, the calcium carbonate skeletons produced by scleractinian corals provide habitat that supports a high biodiversity of fishes and invertebrates. Ocean acidification (OA), driven by excess anthropogenic CO₂ uptake, causes declines in seawater pH and carbonate ion concentration and can compromise coral calcification by causing increased energetic demands. Deciphering how corals meet this increased energetic demand is critical to predicting their future persistence. Oculina arbuscula is a facultatively symbiotic temperate coral common on subtropical reefs of the South Atlantic Bight. This coral has demonstrated calcification resilience to reduced pH conditions in both symbiotic and …

Identification Of Corrosion Damage, Vibration, And Loose Conections In Aircraft Data Transmission Lines Using Reflected And Transmitted Signals; Formulation Of Vegetable Oil-Based Nanofluids As Cutting Fluids For Mql Machining, Saidanvardzhon Valiev

Doctoral Dissertations

This research presents a novel Frequency Domain Transmitometry (FDT) method that uses transmitted signals (S21) to detect and characterize aircraft data transmission lines (ADTL) corrosion damage without additional reflectometry circuitry. Corrosion experiments were conducted according to ASTM G85-A5 over 14 weeks. Combined FDT and reflected signals (S11) analyses revealed distinct three-peak signatures associated with damage and corrosion. The square root of the Area Under the Curve (AUC) of the FDT damage peak and the Full Width at Three-Quarter Maximum (FW3QM) of the S11 damage peak is correlated with corrosion propagation depth and width. S11 signals were further used for vibration …

Perceived Credibility Of Allegations Of Sexual Assault Across Victim Race And Mental Health History, Liyah C. Morgan

College of Graduate Studies: Theses & Dissertations

Previous research examined the effect of victim gender and rape myth acceptance (RMA) on perceptions of victim credibility; however, little attention has been given to the impact of victim race and mental health history, and even fewer studies have explored the intersection of these two identities. The primary purpose of the current study was to identify factors affecting understanding of perceived credibility of victims of sexual assault, specifically in terms of victim race and mental health history. The study also examined the role of RMA on perceptions of credibility and explored participant attitudes related to color-blind racial attitudes and mental …

Application Of Artificial Intelligence Techniques To Improve Leadership Decision Making With Uncertainty, Michael David Parrish

Doctoral Dissertations

"Every good leader is a good manager, but not every good manager is a good leader. The difference between the leader and the manager is critical decision-making. Today’s decision-making environment is characterized as Volatile, Uncertain, Complex, and Ambiguous (VUCA). With the exponential increase in the technical capabilities of systems, the human has become the weakest link in the use of such systems. To remain relevant, good leaders must continuously adapt to new advances in technology and processes.

The research contributions of this work provide several unique and novel solutions for leaders to utilize artificial intelligence tools to improve and optimize …

Raft-Based Polymer And Nanoparticle Materials For Traumatic Brain Injury Treatment And Diagnostics, Aaron Priester

Doctoral Dissertations

"Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. Neurodegenerative diseases that develop post-TBI can be, in part, attributed to DNA and cell-damaging reactive oxygen species (ROS) and lipid peroxidation products (LPOx). This thesis focused on overcoming the limits of current TBI material treatment approaches by employing a RAFT (reversible-addition fragmentation chain transfer) polymer approach that incorporated novel therapeutic, diagnostic and peptide-targeting monomers. An improved nanoparticle synthesis approach was also developed. Thiol and thioether-containing monomers neutralize both ROS and LPOx while Gd-containing monomers with enhanced magnetic resonance imaging (MRI) contrast provide diagnostics and material tracking in …

Lifelong Machine Learning With Adaptive Resonance Theory, Sasha Petrenko

Doctoral Dissertations

"This publication option dissertation is composed of three papers concerning the study of the problem lifelong machine learning with Adaptive Resonance Theory (ART) algorithms. Lifelong learning (L2) is a challenging machine learning paradigm that both encompasses and formalizes the fields of continual learning and incremental learning. The field is concerned with the mitigation of the phenomenon of catastrophic forgetting whereby learning agents that are faced with incrementally novel information deleteriously overwrite previous knowledge if that learning process is not regularized to counteract this consequence. ART algorithms solve this stability-plasticity dilemma by optimally assigning learning to categories or instantiating new knowledge …

Implication And Applications Of Machine Learning On Biomedical Images, Jason Hagerty

Doctoral Dissertations

Medical imaging ranges in modality including computer tomography imaging, x-ray imaging, digital microscopy, and macro-focus dermoscopy images. The latter two modalities are the focus of the presented work.

To perform a diagnostic evaluation on the captured dermoscopy image, it begins with what is usually a labor-intensive operation that requires an expert to perform the initial segmentation for localizing a region of interest (ROI). Once that ROI is obtained, a physician with years of training and experience will observe biological markers that can be used to visually differentiate whether a lesion is benign or malignant. A similar process is used for …