Digital Commons Network^™

The Multifaceted Roles Of Propionic Acid In Autism Spectrum Disorder And Crohn’S Disease, Piotr P. Lagod

Graduate Thesis and Dissertation post-2024

Short chain fatty acids (SCFAs), including propionic (PPA) and butyric (BA) acid, exert a variable and organ-specific effect on the body. They are by-products of human microbiota metabolism and are often used as a food preservative. Studies have shown that PPA and its producers are elevated in autism spectrum disorder (ASD) patients and their mothers. Our lab previously demonstrated that PPA alters neural stem cell differentiation in favor of glia. In this work, we utilized an in vivo mice model, in which dams and their offspring were fed a PPA-rich diet. In the brain, we observed a significant increase in …

Molecular Basis For The Disaggregase Activity Of Protein Disulfide Isomerase, Antonio Mele

Graduate Thesis and Dissertation post-2024

Protein disulfide isomerase (PDI) is an essential, multifunctional, and promiscuous protein with a modular abb’xa’c domain arrangement. Conformational flexibility and the independent, yet coordinated, functionality of PDI domains facilitate protein folding, redox regulation, and the maintenance of cellular homeostasis. Previous investigations have shown that PDI can inhibit amyloid β peptide aggregation, associated with Alzheimer's disease, and is required for cholera toxin disassembly, the rate-limiting step in Vibrio cholerae pathogenesis. However, the precise mechanistic details underlying these interactions remain to be elucidated. Using molecular and biophysical approaches, this study has begun to establish substrate-induced unfolding, or conditional disorder, of PDI as …

Encapsulation Of Phytochemicals Through Polymer-Catechin Nanoparticles Self-Assembled Via Tea-Steeping, Sawyer S. Chang

Graduate Thesis and Dissertation post-2024

The brewing of teas has been an integral pillar of human food culture for millennia. These beverages have always aimed to extract the phytochemicals from berries, leaves, and many other food products. Leaves from Camellia sinensis are used to prepare green, black, white, oolong, and pu’er teas that are highly regarded for their organoleptic properties. Moreover, many of the widely regarded health benefits are ascribed to the high polyphenolic content within the teas. It has been established that polyphenols can interact with hydrophilic polymers, through hydrogen bonding, to form stable small particle suspensions. In this work, polymer-catechin nanoparticles were synthesized …

Catching Light Finding Encouragement Through Art And The Role Of Women In Impressionism, Vlada Evtushenko

Graduate Thesis and Dissertation post-2024

Catching Light is a two-dimensional (2D) animated film about the importance of human connection through the tale of an artist rediscovering their passion for painting through the support of a fellow artist. Catching Light is told through the lens of the period in art history when the artists, who eventually came to be known as 'Impressionists', broke free from the traditions of the Academy. The story highlights how the Art Academy restricted and discriminated against people based on social class and gender, and its disrespect towards the craft of paintmaking. By setting this story in the late 19th century, Catching …

Development Of Human Neuronal Microphysiological Models And Subsequent Integration Into Multi-Organ Platforms For Evaluation Of Therapeutic Efficacy And Off-Target Toxicity, Aakash Patel

Graduate Thesis and Dissertation post-2024

Microphysiological systems are a rapidly growing field of research for modeling of physiological processes and disease in an in vitro environment. The translatability and cost effectiveness of these platforms make them a valuable tool for drug discovery, allowing investigation of both efficacy and off-target toxicity either as a replacement or supplement to traditional animal models. Neurological disorders in particular are often difficult to emulate in an animal model, making the development of these models a highly useful area of research for both elucidation of brain function, as well as neurological disorders. Taking these models and integrating them into a multi-organ …

On The Pedagogy Of Modes, Joshua M. Sanfilippo

Graduate Thesis and Dissertation post-2024

Undergraduate music students in their junior and senior years have a solid foundation in music fundamentals based on the four-semester music theory sequence taken during their sophomore and junior years, but most have a weakness in the area of diatonic modes. This is not to say that they are unaware of the modes or that they do not understand how to identify or construct them, but rather that students are unable to conceptualize modality in a pre-tonal context. To the modern undergraduate music student, the modes blossomed out of the major scale—a notion counter to historical truth. This backwards way …

Mat 1320g : Mathematical Modeling, Mathematics And Computer Science

Approved Course Proposals

No abstract provided.

Swk 4570 : Social Work Practicum I, Human Services

Approved Course Proposals

No abstract provided.

Developing Novel Therapeutic Strategies For Mcrc With A Brafv600e Mutation, Mariah Geisen

Theses and Dissertations--Toxicology and Cancer Biology

Colorectal cancer (CRC) is the second leading cause of cancer death worldwide. The overall incidence rates of CRC have decreased in those over 50, but there is an increasing incidence rate in those under 50. Risk factors for CRC include diet, family history, and a sedentary lifestyle. Early detection of CRC is key to patient survival and treatment response. Localized CRC has a survival rate of 90% and allows for surgical resection and chemotherapy. The 5-year survival for metastatic CRC (mCRC) is significantly reduced to around 14%. Despite early detection, 50% of patients will develop metastasis after surgical resection and …

Nf-Κb-Mediated Oxidative Stress Drives Cigarette Smoke-Induced Emt In Human Bronchial Cells, Sarah M. Alqithami

Theses and Dissertations--Toxicology and Cancer Biology

Cigarette smoke contains over 7,000 chemicals, many being carcinogens and proinflammatory agents contributing to chronic respiratory diseases like COPD and lung cancer. This study investigated responses of human bronchial epithelial cells (HBECs) to cigarette smoke condensate (CSC), focusing on oxidative stress and NF-κB signaling. Three HBEC lines were exposed to non-cytotoxic CSC doses for 48 hours, inducing morphological changes consistent with epithelial-to-mesenchymal transition (EMT). RNA sequencing revealed transcriptomic shifts in all cell lines, particularly affecting genes related to oxidative stress, inflammation, hypoxia, and metabolism. Enrichment analyses confirmed activation of NRF2 antioxidant, NF-κB and IL-17 inflammatory, and hypoxiainducible factor pathways, indicating …

Elucidating The Adverse Effects Of Chronic Pfos Exposure On Gastrointestinal Pathology And Colorectal Cancer, Jerika Durham

Theses and Dissertations--Toxicology and Cancer Biology

Commonly referred to as "forever chemicals," per- and polyfluoroalkyl substances (PFAS) have been linked to a number of detrimental health effects, including an elevated risk of cancer. One "long-chain" subtype of PFAS, perfluorooctanesulfonic acid (PFOS), has a long elimination half-life and a significant propensity for bioaccumulation. Because PFOS is commonly found in drinking water, the gastrointestinal system absorbs it at a high rate. Recent studies demonstrate that PFAS exposures promote intestinal inflammation and gut barrier dysfunction. However, how a long-term PFOS exposure affects colorectal cancer (CRC) progression is not known. Therefore, the purpose of this research is to understand how …

Investigating Plk1 In Pulmonary Fibrosis, Tempany Arbogast

Theses and Dissertations--Toxicology and Cancer Biology

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease, with a median survival of only 2–3 years following diagnosis. In the United States, the disease contributes to more than 40,000 deaths annually, underscoring its severe clinical burden. Alveolar type II (AT2) lung epithelial cells serve as progenitors that maintain alveolar integrity by replenishing epithelial populations and producing surfactants, while fibroblasts and myofibroblasts regulate the extracellular matrix and structural support. In pulmonary fibrosis (PF) injury to AT2 cells promotes cytokine and growth factor release, driving inflammation, fibroblast proliferation, myofibroblast accumulation, and excessive extracellular matrix deposition. Aberrant AT2 cell …

Hss 404-006: Biotechnology And The Law, Mary X. Mitchell

History Syllabi

No abstract provided.

Pfas Litigation And Industry “Science”: Combating Deceptive Industry Practices, Dayna Smith

Western New England Law Review

PFAS have become a hot spot of litigation due to their growing notoriety as one of the greatest chemical threats of our time. Yet, due to concerted efforts by the PFAS industry, PFAS cases are likely to face a significant hurdle in the courtroom. Historically, industry groups have funded studies to lend support to their products, leading to a high risk of bias that has potentially far-reaching consequences. Further, there is mounting evidence that the PFAS industry actively used funding to manipulate science, while also engaging in other deceptive practices to hide potential health risks. This industry “science” then makes …

Revolutionizing The Immaculate Conception: The Limitations Of Medieval Theology And The Advancements Of John Duns Scotus, Emily G. Knoppe

Graduate Theses/Dissertations

Although in the modern era, the Immaculate Conception is a controversial doctrine that prompts conflict primarily between Catholic and non-Catholic groups, during the medieval period Roman Catholics were greatly divided on the topic. Opposition to the belief hinges not only on its lack of scriptural basis and complicated theology, which some deem as placing Mary outside of the need for Christ’s redemption, but also on the fact that it was not declared dogma until 1854 by Pope Pius IX. In this thesis, I first consider how Augustine of Hippo’s original sin theology laid the foundation for avid rejection of the …

A Cadaver Story: Relationality In The Anatomy Laboratory, Stephanie Teasley

Graduate Theses/Dissertations

Cadavers in the human anatomy laboratory occupy a liminal social space, a circumstance that presents a variety of challenges for anatomy students. Cadavers are intended to be scientific objects for study, but their human status makes dissection uniquely challenging. Many of the obstacles to working in the laboratory result from discomfort experienced at the thought of cutting a person (i.e. a social being). In this project, I use ‘cadaver’ to indicate a category of being created in and for the anatomy laboratory. This is distinct from the more general ‘dead body’ in that the category cadaver enables emotional distance and …

Movement And Activity Of Burbot, (Mizay; Lota Lota) In A Spring-Fed Minnesota Lake, Abigail M. Fountain

Biology Graduate Theses

Burbot (Mizay; Lota lota) are a relatively unmanaged freshwater fish, important in many local Indigenous cultures, but often overlooked or maltreated in research, conservation, and fishing practices. As such, relatively little research has been conducted on Burbot, despite ecological significance as an indicator species. A recent resurgence in popularity as a target species, however, has prompted new research to inform management policies. The objectives of this study were to summarize Burbot movement by identifying patterns in swimming activity and identify possible predictors of the movement observed. This study investigated the movements of 39 individuals implanted with acoustic transmitters in Bad …

A Vexing Case: A Newly Discovered Autoinflammatory Disease, Ashley Edwards, Md, Caroline Mcculley

Books, Presentations, Posters, Etc.

Introduction: Vacuoles, E1 enzyme, X-linked, autoinflammatory, somatic (VEXAS) Syndrome is a recently recognized adult-onset autoinflammatory disease that can lead to potentially lifethreatening multi-system inflammation with estimated prevalence of up to 1 in 5,000 males over the age of 50. Given its significant morbidity and mortality and high prevalence, increased awareness of this condition is of foremost importance. Herein we outline a case of VEXAS syndrome. Case Presentation: Mr. N is a 73-year-old male with a past medical history significant for hypertension and recent episode of uveitis who first presented to the hospital for significant unintentional weight loss, fevers, and abdominal …

Teaching Humanities To Medical Students: Moving From The Classroom To The Clinic, Andrew S. Freiberg, Cheryl Dellasega, F. Jeffrey Lorenz, Matthew Darok, Maria Holstrom-Mercader

Health Professions Education

Purpose: While humanistic practice is an essential component of medical school curricula, there are few applied experiences that instill humanistic behaviors. Since 2008, we have offered an innovative clinical rotation, where instead of clinical duties, students “shadow” pediatric oncology patients and their families, employing narrative medicine to provide insights into the experience of illness, navigating health systems issues, and the complexity of interdisciplinary care.

Methods: Using qualitative methods, a trained research team analyzed the 120 student narratives submitted from 2008 through 2019. Statements that exhibited humanism were extracted and reduced to five common themes.

Results: The narratives …

Analysis Of Mutations In Bmpr2 Gene Associated With Hypertension, Elaine Vanterpool, Keury C. Ruiz

Student Posters

Mutations in the BMPR2 gene are primarily recognized for their role in the development of blood vessels, however, they have also been associated with the pathogenesis of high blood pressure, specifically in regards to altered vascular function and the endothelial cell signal. Early detection and targeted treatment, as well as lifestyle changes, are pivotal in reducing the risk of serious complications associated with high blood pressure. Recent genetic research and personalized medicine have demonstrated the potential to enhance the prevention, diagnosis, and treatment of high blood pressure, particularly through the identification of genetic mutations in the BMPR2 gene.

Analyzing The Inhibition Of Chemical Compounds On Protease In P. Aeruginosa, Elaine Vanterpool

Student Posters

Pseudomonas aeruginosa is a gram-negative, bacillus-shaped opportunistic pathogen, notorious for invading immunocompromised individual leading to secondary infections. These opportunistic microbes typically are implicated mild to more severe infections including burn or wound infections to more serious systemic infections. The bacterium Ps. aeruginosa utilizes a variety of virulence factors to enhance the pathogenicity of the organism. Virulence factors secreted by Ps. aeruginosa include its pili, adhesins, capsule, exotoxin A, and lipopolysaccharide (LPS). The proteases produced by Ps. aeruginosa are powerful virulence factors that can lead to tissue destruction and immune invasion. It is imperative that we find ways of regulating the …

Gata1 Gene Variants Associated With Myeloproliferative Syndrome And Its Impact On Hematopoiesis, Elaine Vanterpool, Marliza Mendez Del Orbe

Student Posters

Myeloproliferative syndrome (MPS) are rare blood cancers that originate from an abnormal mutation in a stem cell within the bone marrow, leading to excessive production of red blood cells, white blood cells, or platelets. These disorders affect approximately 20,000 individuals annually in the United States, with around 295,000 people currently living with the condition. The purpose of this study is to identify and assess the pathogenicity of GATA1 gene variants associated with MPS.

An Analysis Of Ftl Variants Associated With Cataracts Disease, Elaine Vanterpool, Joram Kisaka

Student Posters

Cataract is an eye disease that clouds the eye's natural lens, which is used to focus light on the retina. This eye disease causes symptoms such as blurry vision, double vision, sensitivity to light, and difficulty seeing at night. Cataracts are tied to the gene called Ferritin Light Chain (FLT), which is found in both prokaryotes and eukaryotes and plays a role in encoding the light subunits of the ferritin protein. The purpose of this research study was to identify the Ferritin Light Chain mutations that cause cataracts.

An Examination Of Lacc1 Variants Linked To Rheumatoid Arthritis, Elaine Vanterpool, Rochelle Gunter

Student Posters

Rheumatoid arthritis is an inflammatory disease with an annual diagnosis rate of 70 cases per 100,000 people. This is a disease that is usually identified by the abnormal entry of immune cells, such as B lymphocytes, T lymphocytes, and innate lymphoid cells, into the synovial tissues of the joints. When these immune cells infiltrate the joints, they can become too active, thereby contributing to the chronic inflammatory response characteristic of the disease. The objective of this study is to identify and evaluate the pathogenic potential of LACC1 variants implicated in the pathogenesis of arthritis. Simple ClinVar identified LACC1 as a …

The Duality Of The Bard1 Gene, Elaine Vanterpool, Khadra Burden

Student Posters

Breast cancer is the 2nd most diagnosed cancer in women in the United States (Mayo Clinic). Cancer is the overproduction of cells. In other words, cancer-infected cells continue to grow and may cause large growths called tumors. Although there have been innumerable strides in breast cancer research, the exact cause for breast cancer is not yet known (Mayo Clinic). It is believed that factors such as family history of the disease, early menstruation age, and obesity can increase an individual’s chances of having breast cancer. One of the well-known genes associated with breast cancer is BRCA1. A much less researched …

An Analysis Of Androgen Receptor (Ar) Variants In Breast Cancer, Elaine Vanterpool, Jordae Smith

Student Posters

The androgen receptor (AR) is a steroid-hormone activated transcription factor that regulates gene expression in response to testosterone and dihydrotestosterone (DHT). While AR has been extensively studied in prostate cancer, emerging research suggests its role in breast cancer, particularly in estrogen receptor-positive (ER-positive) and triple-negative breast cancer (TNBC). This study investigates AR variants associated with breast cancer, assessing their pathogenicity and potential impact on prognosis and treatment.

Study Of Myl3 Mutation Variants And Their Association With Cardiac Amyloidosis, Elaine Vanterpool, Kyla Tucker

Student Posters

Cardiac Amyloidosis is a rare serious, pathological, clinical progressive disease that is caused by the buildup of amyloid fibrils deposited at the cardiac level. The excess amyloid fibrils deposited in the myocardium leads to this critical condition. Cardiac amyloidosis is best treated when diagnosed in its initial stages. The purpose of this study was to identify and assess the clinical significance and pathogenicity of the Myosin light chain 3 (MYL3) its variants, and the potential link to cardiac amyloidosis. For this study, Simple ClinVar was utilized to identify MYL3 as one of the genes correlated with Cardiac Amyloidosis and variants. …

Silver Nanoparticles And Natural Compounds Can Modulate Proteolytic Activities Of Escherichia Coli, Elaine Vanterpool

Student Posters

Escherichia coli (E. coli) is a gram-negative, facultative anaerobic bacterium that naturally resides in the human gastrointestinal tract as a commensal microorganism. While non-pathogenic strains contribute to digestion and vitamin production, certain pathogenic strains can cause a wide range of diseases, from mild gastrointestinal discomfort to severe illness. A key factor in its pathogenicity is the production of protease enzymes that break down proteins which play a crucial role in bacterial survival and virulence. This study aimed to investigate the effectiveness of different antibacterial compounds in inhibiting the function of both secreted and cell-associated E. coli protease activity.

Analysis Of Kcnj2 Variants Associated With Wolff-Parkinson-White Syndrome, Elaine Vanterpool, Kerenlaime Bautista

Student Posters

Wolff-Parkinson-White (WPW) syndrome is a cardiac disorder characterized by an accessory electrical pathway, leading to tachycardia and arrhythmias. It is diagnosed via electrocardiogram (ECG), showing a shortened PR interval and delta wave. WPW is typically managed with medications, catheter ablation, or surgery. Although primarily caused by anatomical anomalies, emerging research suggests genetic factors, including ion channel mutations, may contribute to the condition. The KCNJ2 gene encodes an inward-rectifier potassium channel critical for cardiac excitability. Mutations in KCNJ2 are linked to Andersen-Tawil syndrome, a disorder featuring arrhythmias and muscle weakness, raising questions about its role in WPW. This study investigates whether …

An Analysis Of Htt Involvement In Huntington’S Disease, Elaine Vanterpool, Micah Andrews

Student Posters

Huntington's disease is a neurodegenerative disorder characterized by loss of striatal neurons. These striatal neurons are essential in cognition, motor function, decision-making, motivation, reinforcement, and reward perception. Affected individuals may show symptoms such as isolation, lack of motivation, depression, mood swings, and personality changes. This study’s purpose is to analyze and highlight the variants of the HTT gene in the pathogenesis of Huntington’s disease.