Digital Commons Network^™

Macabre Therapeutics: An Exploration Into The Cathartic Nature Of Splatter Utilizing The Saw Franchise As Corpus, Rosalind E. Rohrbaugh

Graduate Thesis and Dissertation post-2024

This project positions splatter (a subgenre of horror films that centralizes gore) as applicable for sociopolitical critique and emotional catharsis, disrupting the narrative of splatter as a solely shock-based enterprise. The project utilizes the Saw franchise as a corpus, which reignited audience interest in extreme horror in 2004, rejuvenating splatter film releases since their stagnancy throughout the 1990s. This research seeks to answer why Saw captured the attention of audiences and returned the splatter subgenre to the 21st century, utilizing psychoanalytical film analysis, sociopolitical analysis, semiotics, content analysis, and archival research as methodology and drawing on Aristotle’s Catharsis and Rhetorical …

Principles Of Biomedical Research, Faina Linkov, Matthew C. Kostek

Open Education Materials

HLT 430/530

CC BY-NC-SA

Romantic Beliefs And Their Effect On Relationship Satisfaction, Psychological Well-Being, And Psychological Distress, John D. Pinsky

PCOM Psychology Dissertations

Relationship satisfaction has been shown to have a powerful effect on both individual psychological well-being and psychological distress. One factor that influences an individual’s relationship satisfaction is their endorsement of romantic beliefs. This finding has not been replicated in the U.S. in the past 20 years and the influence of romantic beliefs on relationship satisfaction, psychological well-being, and psychological distress has not been studied in depth. This study implemented a quasi-experimental design using online self-report measures to test whether level of endorsement of romantic beliefs can predict relationship satisfaction, psychological well-being, or psychological distress. It was hypothesized that romantic beliefs …

Stachys Matthewsii: Photo Essay Of A Rare Plant, W. John Hayden

Biology Faculty Publications

On the morning of June 14, 2021, while collecting bryophytes in Goochland County with my student, Mikayla Quinn, we stumbled upon a previously unknown population of stachys matthewsii, Yadkin's Hedge Nettle.

What’S In A Name? Anapodophyllon? Podophyllum? It’S Still Mayapple, W. John Hayden

Biology Faculty Publications

No abstract provided.

Effects Of Starter Cultures On The Metabolite Profile, Antioxidant Activities, And Anti-Aging Properties Of Tapuy Lees, Edward Kevin B. Bragais, Paul Mark B. Medina

Biology Faculty Publications

The Philippine rice wine (tapuy) and tapuy lees making process utilizes bubod, the traditional starter culture. The bubod contains variable number and species of microorganisms and influences the physical and chemical properties of tapuy and tapuy lees, resulting in inconsistent quality products. Hence, improvement and standardization of local starter culture are significant. Here, we examined the effects of well-defined starter cultures (Rhizopus oryzae (RO), Mucor indicus (MI), and Saccharomyces cerevisiae (SC)) and bubod on the metabolite profile, antioxidant, and anti-aging activities of tapuy lees. Our results showed the presence of polyphenols (102.36 ± 2.84 to 173.92 …

In Silico Screening And Identification Of Ctl And Htl Epitopes In The Secreted Virulence Factors Of Mycobacterium Tuberculosis, Edward Kevin B. Bragais, F. M. Heralde Iii, K. C. Fernandez, S. E. Caoili

Biology Faculty Publications

Background: Mycobacterium tuberculosis (MTb) is a highly infectious pathogen and a global health threat due to its resilient cell wall and immune evasion strategies. Despite the availability of the antituberculosis Bacille Calmette-Guérin (BCG) vaccine, its efficacy varies (0%–80%) and gradually decreases over time. This study aimed to identify cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes in MTb secretory proteins using immunoinformatics tools. Material and methods: The Protein Variability Server was used to identify highly conserved sequences, and epitope population coverage was estimated for the Southeast Asian (SEA) region. Selected epitopes were also docked to their major histocompatibility complex alleles. …

In Silico Prediction Of Cytotoxic T-Cell Epitopes From Helicobacter Pylori Virulence Factors Using An Immunoinformatics Approach, Demy Valerie Chacon, Kiana Alika Co, Daphne Noreen Enriquez, Aubrey Love Labarda

Biology Faculty Publications

Background: Helicobacter pylori infects approximately half of the global population, leading to gastric and duodenal ulcers. Despite the availability of antibiotics, challenges such as patient reluctance, high treatment costs, and antibiotic resistance limit their effectiveness, making vaccination a promising alternative. This study used immunoinformatics to identify candidate epitopes for a multiepitope vaccine construct against H. pylori. Material and methods: The protein variability server was utilized for conservation analysis. The epitopes were screened for antigenicity, allergenicity, toxicity, cross-reactivity, and population coverage. Selected epitopes were docked with their corresponding human leukocyte antigen (HLA) alleles, and thermodynamic quantities were determined. Five virulence factors …

Development And Pilot Of A Microgreens Project To Integrate Food Security In Grade 8 Science Using Systems Thinking, Iris Mae L. Caidic, C. K. Gonzales

Biology Faculty Publications

Among other countries, the Philippines faces the issue of food security, which must be addressed through education. However, the lack of coherence in teaching science content hinders Filipino junior high school students’ holistic understanding that science lessons are interconnected to food security. This study explores how systems thinking can be utilized to integrate food security into science topics. The pilot of the developed module on a microgreens project was implemented using systems thinking tools to tackle lessons on biodiversity, ecosystems, and the human digestive system within the context of food security. Data from end-of-scaffold check-ins, individual scaffold assessments, group performance …

A Concoction Pipeline For Generating Molecular Operational Taxonomic Units (Motus) Among Riparian And Aquatic Beetles, Emmanuel D. Delocado, Voltaire Rafael H. Banzon, Enrico Gerard S. Sanchez

Biology Faculty Publications

No abstract provided.

A Low-Cost Production Method For Bst Dna Polymerase (Large Fragment), Crisanto M. Lopez, M. V. San Pedro, V. A. Cañas, J. R. Garcia

Biology Faculty Publications

BstLF, the large fragment of Bst DNA polymerase, is a recombinant strand-displacing DNA polymerase derived from Geobacillus stearothermophilus and serves as the primary enzyme utilized in loopmediated isothermal amplification (LAMP). Recently, the application of LAMP as a convenient and accessible diagnostic tool has gained considerable attention. However, the high cost of reagents presents a significant barrier to the development of LAMP diagnostic kits, limiting accessibility for laboratories in low-resource settings. In this study, we report a low-cost production method for BstLF utilizing more readily available alternative reagents coupled with a simplified purification process. The resulting homebrewed BstLF demonstrated comparable amplification …

Assessment Of Validity And Reliability Of Survey Tool For Good Aquaculture Practices Of Philippine Shellfish Farmers, Crisanto M. Lopez, A. Rustia, E. Barrios, D. Tanay

Biology Faculty Publications

Food safety farm-to-fork model compliance to good aquaculture practices (GAqP) of Filipino shellfish farmers placed a special consideration in the validation and reliability of the developed survey tool that consisted of 11 sections of GAqP provisions based on Philippine National Standards 207:2017 and CXS 52-2003 standards. Validation [content validity index (CVI) and impact factor (IF)] and reliability (Cronbach’s alpha) included pre-testing (item difficulty and discrimination index) (n = 7 shellfish farmers; n = 7 technical personnel on fisheries), validation (n = 30 shellfish farmers and five experts), and pilot testing (n = 30 shellfish farmers) to measure the reliability. Pre-testing …

Dr. Wolfram Mey’S Stonefly (Insecta: Plecoptera) Collection From Luzon, Philippines: Part Ii. Leuctridae, Arthien Lovell P. Pelingen

Biology Faculty Publications

Leuctrid specimens were examined from a collection expedition conducted over 20 years ago by Dr. Wolfram Mey. Rhopalopsole luzonensis sp. nov., R. absentia sp. nov., and R. rotundus sp. nov. are described based on male specimens. Additionally, a distribution hypothesis, discussion of female specimens, and an updated checklist are briefly provided.

The Use Of Synthesized Stabilized Nanoparticles Of Selenium As A Feed Additive In Shrimp Litopenaeus Vannamei Diets: Performance, Antibacterial Activity, Gut Microbiota And Immune-Related Genes Expression, Janice A. Ragaza, F. Mustafa, S. Hassan, O. Ashary

Biology Faculty Publications

The shrimp aquaculture industry faces challenges that hinder production, including pathogens and infections from bacteria-causing diseases. The present study aimed to examine the impact of dietary synthesized chitosan stabilized selenium nanoparticles (CSSeNPs) on the growth performance of gut microbiota and immuneassociated genes for shrimp L. vannamei aquaculture. The experiments were accomplished for 12 weeks in triplicate fiberglass circular tanks (200 L). A 300 shrimp post-larvae (PL) weighing an average of 0.14 ± 0.001 g was utilized. Four experimental groups were assigned to 12 tanks. Each tank contained 25 PL. Each group fed with one of the following diets: control (C), …

Native No More? The Catfish Dilemma In The Philippines, Janice A. Ragaza, Alexis Faye A. Olarte, James Darren R. Pelaez

Biology Faculty Publications

The shrimp aquaculture industry faces challenges that hinder production, including pathogens and infections from bacteria-causing diseases. The present study aimed to examine the impact of dietary synthesized chitosan stabilized selenium nanoparticles (CSSeNPs) on the growth performance of gut microbiota and immuneassociated genes for shrimp L. vannamei aquaculture. The experiments were accomplished for 12 weeks in triplicate fiberglass circular tanks (200 L). A 300 shrimp post-larvae (PL) weighing an average of 0.14 ± 0.001 g was utilized. Four experimental groups were assigned to 12 tanks. Each tank contained 25 PL. Each group fed with one of the following diets: control (C), …

From Bait To Breakthrough: How Polychaetes Are Revolutionizing Philippine Aquaculture, Janice A. Ragaza, Irnes Rebien H. Bailon, Rochelle A. Jabines

Biology Faculty Publications

No abstract provided.

Ptp1b Inhibitors Of Marine Sponge-Derived Secondary Metabolites From Dysidea, Irvin B. Rondolo, G. A. Lasam, M. N. Aguila, A. J. De Jesus

Biology Faculty Publications

Diabetes is a chronic metabolic disease with high morbidity and mortality due to its complications. Recently, there has been growing interest in identifying new bioactive compounds, including marinederived secondary metabolites, for potential antidiabetic activities. This study focuses on secondary metabolites from marine sponges from the genus Dysidea that show strong inhibition of protein tyrosine phosphatase 1B (PTP1B), a key target in antidiabetic therapy. Thirteen out of the thirty-one compounds were identified to have promising in silico potentials vs PTP1B: avarol (1), avarone (2), furodysin (3), nakafuran 8 (4), haterumadysin A (5), pyrodysinoic acid (6), avinosol (7), puupehenone (8), α-santonin (9), …

Clerodendrum Kelli (Lamiaceae), A New Species From Carranglan, Nueva Ecija, Philippines, And Lectotypification Of Clerodendrum Mindorense, Adriane B. Tobias, D. J. Ples, J. Patykowski, L. C. Udasco

Biology Faculty Publications

Clerodendrum kelli R.Bustam., A.B.Tobias & Ples—a new species of the genus Clerodendrum L. (Lamiaceae) from the montane forests of Carranglan, Nueva Ecija, Luzon Island, Philippines—is described and illustrated. The species shares morphological features that are uncommon for the genus with both Clerodendrum quadriloculare (Blanco) Merr. (purple abaxial leaf surfaces) and C. mindorense Merr. (reduced fruiting calyx lobes), but differs from both in having lax (vs dense/compact) inflorescences bearing flowers with shorter corolla tubes and reddish-pink (vs green or purple) calyces with ovate (vs triangular or lanceolate) lobes. Additionally, a lectotype for C. mindorense is assigned. Preliminary assessment of its conservation …

New Record And Amended Description Of Bulbophyllum Placochilum (Orchidaceae) With A Key To Bulbophyllum Sect. Alticeptrum Of The Philippines, Adriane B. Tobias, K. E. Boco, J. P. Collantes, J. C. Altomonte

Biology Faculty Publications

Bulbophyllum placochilum J.J.Verm., endemic to Borneo, is newly recorded in the Philippines based on a specimen collected from Mt Malinao, Albay Province, Luzon Island. We provide a comprehensive account of the species, including a detailed morphological description, ecological observations, distribution range, phenology, and photographs. The species is assessed as Vulnerable under the IUCN Red List Categories and Criteria. A key to species of Bulbophyllum sect. Altisceptrum J.J.Sm. in the Philippines is also included.

Inhibition Of Activin A Signaling Slows The Progression Of Barrett's Esophagus Induced Esophageal Adenocarcinoma, Lily A. Giardino

Graduate Thesis and Dissertation post-2024

In 2020, approximately 1.03 billion cases of Gastro-esophageal Reflux Disease (GERD) were reported worldwide, representing a significant global health burden. Chronic GERD is a potent initiator of Barrett’s Esophagus (BE), a precancerous condition in which the squamous epithelium is converted into columnar-like epithelium in the distal esophagus in response to reflux injury. Barrett’s Esophagus is the only known precursor to Esophageal Adenocarcinoma (EAC), a disease with a five-year survival rate of 21%. First line treatments focus on acid suppression and endoscopic mucosal resection but did not result in a decrease of EAC incidence highlighting the importance of new therapeutics. Previous …

Multi-Omics Analysis Of Streptococcus Pneumoniae Serotype 3 Using A Murine Infection Model, Jash Kamlesh Trivedi

Graduate Thesis and Dissertation post-2024

Streptococcus pneumoniae (SPN), a gram-positive encapsulated pathogen, inhabits the human nasopharynx asymptomatically but can cause severe infections, including invasive pneumococcal diseases (IPD). Although targeted by vaccines, serotype 3 (ST3) remains highly prevalent within the United States and globally. Sequence type 180 lineage within Clonal Complex (CC)180 is the predominant lineage of serotype 3 globally. Genetically, Clonal Complex 180 is divided into clade I and II, with clade II recently surpassing clade I in prevalence. However, the molecular mechanisms driving this shift remain poorly understood. Here, we employ a multi-omics approach, combining pangenome analysis and dual-RNA sequencing, to investigate genetic factors …

Comparing Inter-Rater Reliability For Two Coding Methodologies In Meta-Analysis Data Extraction, Christine D. Destefano

Graduate Thesis and Dissertation post-2024

Meta-analyses are critical tools for evidence-based policymaking, but their validity depends heavily on accurate data extraction from primary studies. While previous research has focused on procedural aspects of coding and reliability of effect size extraction, there remains a significant gap in understanding how to improve inter-rater reliability (IRR) for moderator variables that provide crucial context for meta-analytic findings. This study compared two methodological approaches for coding moderator variables: traditional low-inference coding and a novel drill-down questioning technique adapted from database development principles. Using a comparative analysis design, two independent coders extracted data from 18 primary studies included in a published …

Covariate Selection For Rna-Seq Differential Expression Analysis With Hidden Factor Adjustment, Farzana Noorzahan, Hyeongseon Jeon, Yet Nguyen

Mathematics & Statistics Faculty Publications

In RNA-seq data analysis, a primary objective is the identification of differentially expressed genes, which are genes that exhibit varying expression levels across different conditions of interest. It is widely known that hidden factors, such as batch effects, can substantially influence the differential expression analysis. Furthermore, apart from the primary factor of interest and unforeseen artifacts, an RNA-seq experiment typically contains multiple measured covariates, some of which may significantly affect gene expression levels, while others may not. Existing methods either address the covariate selection or the unknown artifacts separately. In this study, we investigate two integrated strategies, FSR_sva and SVAall_FSR, …

The Impact Of Social And Political Capital On Black Male Educational Leaders Lived Experiences With Hiring Practices: A Convergent Parallel Mixed Methods Descriptive Study, Travis L. Brown

Graduate Thesis and Dissertation post-2024

According to Woodson and Pabon (2016, as cited in Bass, 2020) Black males represent less than 2% of the US public education workforce. This is concerning considering that many schools across the nation are becoming increasingly diverse. Scholars such as Bass (2020) have even cited that classrooms “may never completely rebound” (p. 356) from finding Black men to fill vacant teaching positions which could ultimately lead to a career in educational leadership. filling classrooms with available Black men in the profession. This study employed a convergent parallel mixed method design within a descriptive research framework to examine the impact of …

Multilevel Network Governance For United Nations Sustainable Development Goals: The Role Of European Subnational Entities In Urban Resilience, Hasan Isgandar

Graduate Thesis and Dissertation post-2024

This dissertation investigates the role of subnational actors in European Union (EU) multilevel governance networks focused on reducing urban poverty and achieving the United Nations Sustainable Development Goals (SDGs). Focusing on the SDG #1 (No Poverty), SDG #11 (Sustainable Cities and Communities) and SDG #17 (Partnerships for the Goals) it examines the participation and impact of subnational actors in two case studies: Naples, Italy and Krakow, Poland. Using Social Network Analysis (SNA), the research investigates the configuration and performance of the networks created within the framework of the URBinclusion projects, which are part of the URBACT and Urban Innovative Actions …

Health Behavior In A Culture-Driven Society: Using Mixed Methods Research To Examine Medical Pluralism In Zimbabwe, Stephen Mhere

Graduate Thesis and Dissertation post-2024

This dissertation examined the role of cultural influences on health-seeking behaviors and healthcare choices among Zimbabweans, focusing on medical pluralism – where individuals simultaneously access traditional, faith-based, and allopathic medical systems. It applied the PEN-3 Cultural Model to examine Cultural Identity, Relationships and Expectations, and Cultural Empowerment. Using a convergent parallel mixed-methods design, data were collected via a Shona-language survey on WhatsApp. Multiple choice, closed-ended, and open-ended survey questions were texted to participants, and they responded by text or audio messages. Response data were collected from 137 study participants. Multinomial logistic regression was used for quantitative data analysis, while deductive …

Eating Disorder Patient Experiences With Best Practices During Psychiatric Treatment: An Exploratory Sequential Mixed Methods Study, Pamela L. Dow

Graduate Thesis and Dissertation post-2024

Eating disorders are among the deadliest mental health disorders and represent a significant financial burden to the U.S. healthcare system. However, little is known about eating disorder patients' experiences during psychiatric treatment. Informed by the Picker Principles of Person-Centered Care and recommendations from the American Psychiatric Association, this study explored eating disorder patient experiences with best practices during psychiatric treatment. First, 10 patients were interviewed about their treatment experiences with a psychiatrist. Themes were analyzed using iterative categorization in Dedoose and informed a quantitative survey to examine patient experiences with their psychiatrist performing best practices during eating disorder treatment. The …

Examining The Impact African American Male Teachers Have On The Math Scores Of Underserved Students, Demetrice Thomas

Graduate Thesis and Dissertation post-2024

This study contributed to a growing body of evidence emphasizing that teacher identity profoundly influences not only how students learn but also how they perceive themselves and their potential. As Gershenson (2022) noted, “Black teachers provide benefits to all students, fostering higher achievement and stronger aspirations,” highlighting the essential role of diverse educators in shaping student success. Consistent academic gains observed among students taught by African American male teachers reinforce the urgent need for educational systems to prioritize equity not only in student outcomes but also in diversifying the teaching workforce. Despite African American male teachers comprising less than 2% …

Cigarettes, Flesh, & Other Cravings, Gabrielle F. Decker

Graduate Thesis and Dissertation post-2024

Cigarettes, Flesh, & Other Cravings is a collection of short stories that explores themes of motherhood, death, and desire. Each character struggles with the friction between society's expectations and their desires for happiness, all through the lens of the surreal and the absurd. A destructive raccoon’s nightly visits unravel a woman’s sense of control. A teenager begins an internship with a conspiracy-obsessed clown. A minuscule old woman tumbles out of a chicken egg. A fissure in a kitchen floor births an imp. A Choose Your Own Adventure examines the illusion of choice. In Hell, torture is personalized. Cigarettes, Flesh, & …

Methods And Applications Of Coherent Analog Photonic Processing, Andrew B. Klein

Graduate Thesis and Dissertation post-2024

Photonic processing is a compelling approach to address the increasing computational requirements of neural networks, promising increased processing speed and better energy efficiency for operations such as matrix-vector multiplication. However, in practice the limitations of photonic hardware necessitate that these systems adopt fixed-point encoding rather than the floating-point standard used by digital electronics. The reduction in accuracy incurred by this requirement must be managed and addressed for real world applications. In this work, we present a reconfigurable, scalable, and parallelizable photonic multiplier cell using coherent balanced photodetection of analog signals to produce signed multiplications. This style of system outperforms other …