Digital Commons Network^™

Neur 110n.01: Introduction To Brain Diseases, Beverly J. Piggot

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Membrane Filtration Of Nanoscale Biomaterials: Model System And Membrane Performance Evaluation For Aav2 Viral Vector Clarification And Recovery, Mara Leach, Kearstin Edmonds, Emily Ingram, Rebecca Dutch, Sumith R. Wickramasinghe, Malgorzata Chwatko, Dibakar Bhattacharyya

Chemical Engineering Faculty Publications and Presentations

The growing demand for viral vectors as nanoscale therapeutic agents in gene therapy necessitates efficient and scalable purification methods. This study examined the role of nanoscale biomaterials in optimizing viral vector clarification through a model system mimicking real AAV2 crude harvest material. Using lysed HEK293 cells and silica nanoparticles (20 nm) as surrogates for AAV2 crude harvest, we evaluated primary (depth filters) and secondary (membrane-based) filtration processes under different process parameters and solution conditions. These filtration systems were then assessed for their ability to recover nanoscale viral vectors while reducing DNA (without the need for endonuclease treatment), protein, and turbidity. …

Magnetic Nanoparticles: Advances In Synthesis, Sensing, And Theragnostic Applications, Adeyemi O. Adeeyo, Mercy A. Alabi, Joshua A. Oyetade, Thabo T.I. Nkambule, Bhekie B. Mamba, Adewale O. Oladipo, Rachel Makungo, Titus A.M. Msagati

All Rsif Scholars' Publications

The synthesis of magnetic nanoparticles (MNPs) via the chemical, biological, and physical routes has been reported on along with advantages and attendant limitations. This study focuses on the sensing and emerging theragnostic applications of this category of nanoparticles (NPs) in clinical sciences by unveiling the unique performance of these NPs in the biological sensing of bacteria and nucleotide sequencing. Also, in terms of medicine and clinical science, this review analyzes the emerging theragnostic applications of NPs in drug delivery, bone tissue engineering, deep brain stimulation, therapeutic hyperthermia, tumor detection, magnetic imaging and cell tracking, lymph node visualization, blood purification, and …

Religion: Evolutionary Foundation For, Nicole Fellice Ko, Norman P. Li

Research Collection School of Social Sciences

Religion refers to a structured set of spiritual beliefs and rituals. Evolution refers to the process by which living organisms transform and develop over time. Religion—the belief in spiritual beings—influences nearly every aspect of life, making it challenging to understand many phenomena without considering its influence (Bloom, 2012). About two-thirds of the world’s population is religious (Kramer & Tong, 2024); 70% of Americans consider themselves spiritual, and 83% believe they have a spirit separate from their bodies (PEW Research Center, 2023). Throughout history, religion has played a significant role in conflicts like war and continues to influence contemporary issues such …

February 2025 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Hydrolysis Of The Acetyl-Coa Allosteric Activator By Staphylococcus Aureus Pyruvate Carboxylase, Amanda J. Laseke, Jeremy R. Lohman, Martin St. Maurice

Biological Sciences Faculty Research and Publications

Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate which serves as an important anaplerotic reaction to replenish citric acid cycle intermediates. In most organisms, the PC-catalyzed reaction is allosterically activated by acetyl-coenzyme A. It has previously been reported that vertebrate PC can catalyze the hydrolysis of acetyl-CoA, offering a potential means for the enzyme to attenuate its allosteric activation. However, in the years since this initial report, there has been no further investigation of this phenomenon. The allosteric binding site for acetyl-CoA is now well characterized, enabling more detailed studies on acetyl-CoA hydrolysis at the allosteric site. Here, …

Six-Month Follow-Up Comparing Ait And Eft In The Reduction Of Negative Emotions Associated With A Past Memory, Gregory Brown, Kavita Batra, Emily Dorin, Andrew Han, Arianna Palermini, Robert Sottile, Sherine Khanbijian

Psychiatry & Behavioral Health Faculty Research

The field of Energy Psychology [EP] has amassed hundreds published research studies over its thirty year history which includes both systematic review and meta-analysis level data. These have consistently demonstrated significant effect sizes equivalent to EMDR and trauma based CBT (Church et al., 2022; Feinstein, 2021). Energy Psychology Techniques typically include an energetic component, an emotional component, a somatic component, focus upon a specific memory, which lead to automatic secondary cognitive shifts. Memory Reconsolidation may be a primary psycho-biological explanation for effectiveness. Emotional Freedom Technique [EFT] is the most studied EP technique to date, and considered an evidenced based treatment …

Attenuation Of Vitamin E On Haloxyfop-R-Methyl Ester-Induced Hepatic Oxidative Stress In Orally Exposed Rats., Omolara D. Falodun

STEM for Success Resources

No abstract provided.

Proving The Future In Criminal Cases, Christopher Slobogin

Vanderbilt Law School Faculty Publications

Expert opinion about dangerousness—the risk of reoffending—is commonly introduced at sentencing, criminal commitment proceedings, and some types of pretrial detention hearings. This Essay argues that the rules governing the admissibility of scientific evidence should apply to this testimony and that, on that assumption, such evidence must be (1) “material” (logically relevant, empirically generalizable, and epistemologically germane), (2) “probative” (a measure of accuracy, which is more stringent when the evidence is from an expert), (3) helpful to the factfinder (through promoting “incremental validity”), and (4) presented in a non-prejudicial manner (i.e., in a way that minimizes the possibility it will be …

Characterizing Prodrome (Premonitory Phase) In Migraine: Results From The Prodrome Trial Screening Period, Todd Schwedt, Richard Lipton, Peter Goadsby, Chia-Chun Chiang, Brad Klein, Cory Hussar, Chengcheng Liu, Sung Yun Yu, Michelle Finnegan, Joel Trugman

College of Nursing Faculty Papers & Presentations

BACKGROUND AND OBJECTIVE: Limited data are available describing the frequency, severity, and consistency of prodromal symptoms followed by headache. This analysis of the PRODROME trial screening period characterized prodromal symptoms in people with migraine, including the most common symptoms and their severity, and the frequency and consistency with which prodromal symptoms were followed by headache.

METHODS: PRODROME was a multicenter, randomized, double-blind, placebo-controlled, crossover trial conducted in the United States that enrolled adults with 2-8 migraine attacks per month who stated they could identify prodromal symptoms that were reliably followed by a headache. The trial included a 60-day screening period …

Bioh 447.01: Genes And Development Lab, Ekaterina Voronina

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Bioo 335.04: Rocky Mountain Flora Lab, Evan Stark-Dykema

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Biob 375.01: General Genetics, Sarah J. Certel

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Biob 491.01: Watershed Science Education & Practice, Dalit Guscio

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Bioe 447.01: Ecosystem Ecology, Benjamin P. Colman

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Biomanipulation, Laura K. Donohue

Georgetown Law Faculty Publications and Other Works

Scientific and technological advances in the latter part of the twentieth century transformed the field of biometrics. Carleton Simon, for instance, first postulated using retinal vasculature for biometric identification in 1935, but it was not until forty years later that an Eyedentify patent brought the idea to fruition. In 1937, John Henry Wigmore anticipated using oscilloscopes to identify individuals by speech patterns. Decades later, digitization and speech processors made voiceprint identification possible. In the 1970s, biological discoveries similarly led to the development of deoxyribonucleic acid (DNA) sequencing. And while Alphonse Bertillon in the late nineteenth century postulated iris distinctions, it …

A Switch Protein Adapter For Anti-Lilrb4 Car-T Cells, Ryan Huang, Heyu Chen, Jingjing Xie, Qi Lou, Lingxiao Tan, Ningyan Zhang, Zhiqiang An, Samuel John, Cheng Cheng Zhang

The Brown Foundation: Institute of Molecular Medicine

Chimeric antigen receptor-T cell (CAR-T) immunotherapy has shown remarkable results for the treatment of certain hematologic malignancies. A redirection strategy that utilizes clinically relevant CAR-T cells in combination with adapter proteins may be an effective strategy to target other hematologic and solid cancers. We established a fusion antibody-based strategy with flexibility to target multiple tumor types in combination with a novel anti-leukocyte immunoglobulin-like receptor-B 4 (LILRB4) CAR-T cell. Specifically, we engineered switch protein (SwP) adapters containing the LILRB4 extracellular domain fused to either an anti-CD19 or anti-CD20 single-chain variable fragment (scFv). These SwPs were sufficient to stimulate anti-LILRB4 CAR-T cells …

Leucine-Rich Alpha-2-Glycoprotein 1 Promotes Metastatic Colorectal Cancer Growth Through Human Epidermal Growth Factor Receptor 3 Signaling, Moeez Rathore, Kimberly Curry, Wei Huang, Michel'le Wright, Daniel Martin, Jiyeon Baek, Derek Taylor, Masaru Miyagi, Wen Tang, Hao Feng, Yamu Li, Zhenghe Wang, Hallie Graor, Joseph Willis, Elizabeth Bryson, Christina S Boutros, Omkar Desai, Bianca N Islam, Lee M Ellis, Stephen E Moss, Jordan M Winter, John Greenwood, Rui Wang

Faculty, Staff and Student Publications

Background & aims: Therapy failure in patients with metastatic colorectal cancer (mCRC, ∼80% occur in the liver) remains an overarching challenge. Preclinical studies demonstrated that human epidermal growth factor receptor 3 (HER3) promotes colorectal cancer (CRC) cell survival, but therapies blocking the neuregulin-induced canonical HER3 signaling have made little impact in the clinic. Recent studies suggest that the liver microenvironment promotes CRC growth by activating HER3 in a neuregulin-independent fashion, thus elucidation of these mechanisms may reveal new strategies for treating patients with mCRC.

Methods: Patient-derived primary liver endothelial cells (ECs) were used to interrogate EC-CRC crosstalk. We conducted proteomic …

In Vitro Hydrolysis Of Areca Nut Xenobiotics In Human Liver, Vincenzo Paolillo, Mahendran Jayakumar, Colton Sheperd, Andrew Tran, Stephanie Hoang, Nhu Dao, Parag Jain, Alan L Myers

Faculty, Staff and Student Publications

Areca nut (AN) is a substance of abuse consumed by millions worldwide, in spite of established oral and systemic toxicities associated with its use. Previous research demonstrates methyl ester alkaloids in the AN, such as arecoline and guvacoline, exhibit mood-altering and toxicological effects. Nonetheless, their metabolism has not been fully elucidated in humans. In the present study, an HPLC-UV bioanalytical method was developed to evaluate the hydrolytic kinetics and clearance rates of arecoline and guvacoline in human liver microsomes (HLM) and cytosol (HLC). The bioassay was capable of quantifying arecoline and guvacoline (and carboxylate metabolites arecaidine and guvacine, respectively) with …

Vaxbot-Hpv: A Gpt-Based Chatbot For Answering Hpv Vaccine-Related Questions, Yiming Li, Jianfu Li, Manqi Li, Evan Yu, Danniel Rhee, Muhammad Amith, Lu Tang, Lara S Savas, Licong Cui, Cui Tao

Faculty, Staff and Student Publications

OBJECTIVE: Human Papillomavirus (HPV) vaccine is an effective measure to prevent and control the diseases caused by HPV. However, widespread misinformation and vaccine hesitancy remain significant barriers to its uptake. This study focuses on the development of VaxBot-HPV, a chatbot aimed at improving health literacy and promoting vaccination uptake by providing information and answering questions about the HPV vaccine.

METHODS: We constructed the knowledge base (KB) for VaxBot-HPV, which consists of 451 documents from biomedical literature and web sources on the HPV vaccine. We extracted 202 question-answer pairs from the KB and 39 questions generated by GPT-4 for training and …

Plasma Proteomic Characterization Of Motoric Cognitive Risk And Mild Cognitive Impairment, Gabriela T Gomez, Sanish Sathyan, Jingsha Chen, Myriam Fornage, Pascal Schlosser, Zhongsheng Peng, Jenifer Cordon, Priya Palta, Kevin J Sullivan, Adrienne Tin, B Gwen Windham, Rebecca F Gottesman, Nir Barzilai, Sofiya Milman, Joe Verghese, Josef Coresh, Keenan A Walker

Faculty, Staff and Student Publications

INTRODUCTION: Motoric cognitive risk (MCR) is a pre-dementia syndrome characterized by mobility and cognitive dysfunction. This study conducted a proteome-wide study of MCR and compared the proteomic signatures of MCR to that of mild cognitive impairment (MCI).

METHODS: Participants were classified as MCR using a memory questionnaire and 4-meter walk. We measured 4877 plasma proteins collected during late-life and midlife. Multivariable logistic regression related each protein to late-life MCR/MCI. MCR-associated proteins were replicated internally at midlife and in an external cohort.

RESULTS: Proteome-wide analysis (n = 4076) identified 25 MCR-associated proteins. Eight of these proteins remained associated with late-life MCR …

Anty 101h.01: Anthropology And The Human Experience, David Garret Kerr

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Anty 101h.02: Anthropology And The Human Experience, David Garret Kerr

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Anty 133x.B01: Food And Culture, David Garret Kerr

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Anty 459.01; Archaeology Of The Arctic And Subarctic, Anna M. Prentiss

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Anty 513.01: Seminar In Bioarchaeology & Skeletal Biology, Meredeth H. Snow

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Anty 591.01: Forensic Casework, Katherine S. Baca

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Anty 601.02: Research Design & Proposal Preparation, Anna M. Prentiss

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Bch 380.00: Biochemistry, Scott Samuels

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Bch 482.01: Advanced Biochemistry Ii, J. Stephen Lodmell

University of Montana Course Syllabi, 2021-2025

No abstract provided.