Digital Commons Network^™

The Prevalence Of Cytauxzoon Felis In Southern United States, Anna Haynes

Biological Sciences Undergraduate Honors Theses

Cytauxzoonosis, caused by transmission from a tick infected with the pathogen Cytauxzoon felis, is considered an emerging tick-borne disease in domestic felines. While not as fatal in other vertebrates such as the reservoir host Lynx rufus (bobcat), in domestic cats, infection is fatal nearly 100% of the time. This study sought to screen ticks pulled from felines for the pathogen across three states in an area identified as a hotspot. Out of 80 ticks screened, 12 tested positive for the pathogen. The positive population of ticks came from 3 neighboring counties in Southern Missouri: Jasper, McDonald, and Newton. McDonald …

Antimicrobial Peptides Improve Antibiotic-Mediating Killing Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Biofilms, Alya R. Theriault

All Theses And Dissertations

Staphylococcus aureus can cause drug-resistant, biofilm-associated infections. These infections place methicillin-resistant S. aureus (MRSA) as one of the sixth leading bacterial pathogens associated with deaths due to antimicrobial resistance. Traditional antibiotics are no longer enough to treat these resistant infections and novel therapeutics are needed. Antimicrobial adjuvants are a potential strategy for improving efficacy of existing antibiotics, and antimicrobial peptides (AMPs) may have adjuvant activity. Recent reports showed that AMPs improve antibiotic activity against planktonic staphylococci, but whether AMPs might alter antibiotic efficacy against biofilm-resident staphylococcus is less clear. We tested the effect of nine AMPs on staphylococcal biofilm susceptibility …

Alphamissense Predictions And Clinvar Annotations: A Deep Learning Approach To Uveal Melanoma, David J. Taylor Gonzalez, Mak B. Djulbegovic, Meghan Sharma, Michael Antonietti, Colin K. Kim, Vladimir N. Uversky, Carol L. Karp, Carol L. Shields, Matthew W. Wilson

Wills Eye Hospital Papers

OBJECTIVE: Uveal melanoma (UM) poses significant diagnostic and prognostic challenges due to its variable genetic landscape. We explore the use of a novel deep learning tool to assess the functional impact of genetic mutations in UM.

DESIGN: A cross-sectional bioinformatics exploratory data analysis of genetic mutations from UM cases.

SUBJECTS: Genetic data from patients diagnosed with UM were analyzed, explicitly focusing on missense mutations sourced from the Catalogue of Somatic Mutations in Cancer (COSMIC) database.

METHODS: We identified missense mutations frequently observed in UM using the COSMIC database, assessed their potential pathogenicity using AlphaMissense, and visualized mutations using AlphaFold. Clinical …

Pseudomonas Aeruginosa Exoenzyme Y Enzymatic Activity Inhibits Exoenzyme S-Induced Caspase Activation But Not Cytotoxicity In Pulmonary Microvascular Endothelial Cells, Connor Holm

Honors Theses

Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of ventilator-associated pneumonia in intensive care unit patients. P. aeruginosa utilizes a type III secretion system that injects exoenzymes U, S, T, and/or Y into the host cell cytosol. A majority of clinically isolated P. aeruginosa strains express ExoY often in combination with ExoU and ExoT, or ExoS and ExoT. Host signaling mechanisms in response to intoxication with individual exoenzymes have been extensively studied, yet little is known about host cell response to co-intoxication. In this study, pulmonary microvascular endothelial cells (PMVECs) were infected with P. aeruginosa strains expressing different combinations …

Improving Blood Culture Contamination Rates In The Emergency Department: An Evidence-Based Quality Improvement Project, Kerri Maciejko

DNP Projects

Background/Significance

Blood culture (BC) contamination in the Emergency Department (ED) leads to false-positive results, unnecessary antibiotic use, prolonged hospital stays, and increased healthcare costs. Contamination rates at the project site ranged from 2.2% to 6.8%, exceeding the hospital goal of <1%. Addressing this issue is critical for accurate diagnosis, effective treatment, and optimal resource utilization.

Purpose

The purpose of this project was to reduce BC contamination rates in the ED by implementing a modified BC kit, providing staff education, and conducting real-time blind audits with feedback over three months.

Methods

An evidence review, appraisal, and synthesis using Melnyk and Fineout-Overholt’s EBP process confirmed that BC kits and diversion devices are effective interventions to reduce contamination. …

Which Costimulatory Domains In Chimeric Antigen Receptor T Cells Induce Cytokine Responses That Are Linked To A More Toxic Car T Cell Therapy In Acute Lymphocytic Leukemia?, Elisabeth Cohen

Longwood Senior Thesis Proposal

No abstract provided.

Activity Of Novel Ceftibuten-Avibactam, Ceftazidime-Avibactam, And Comparators Against A Challenge Set Of Enterobacterales From Outpatient Centers And Nursing Homes Across The United States (2022–2024), Aliaa Fouad, April M. Bobenchik, Robin Chamberland, Andrew E. Clark, Holly K. Huse, Isabella W. Martin, A. Brian Mochon, Erik Munson, Maroun M. Sfeir, Monica Srodon, Kimberly Taylor, Yungchou Wang, Lars F. Westblade, David P. Nicolau, Tomefa E. Asempa

Clinical Lab Sciences Faculty Research and Publications

Ceftibuten is being developed in combination with the oral prodrug of avibactam. Outpatient isolates (n = 500) from 11 US states underwent susceptibility testing with manual broth microdilution. Ceftibuten had susceptibility rates of 64% (CLSI) and 23% (EUCAST). Ceftibuten-avibactam achieved 96.4% inhibition at MIC ≤ 1 µg/mL. Susceptibility rates were as follows: cefpodoxime (2.6%), ceftriaxone (1%), ceftazidime-avibactam (99.6%), tebipenem (89%), ertapenem (94.4%), levofloxacin (26.6%), trimethoprim-sulfamethoxazole (40.2%), and fosfomycin (96.8%). Ceftibuten-avibactam demonstrates potent in vitro activity against cephalosporin non-susceptible isolates.

Bang!: Real-Time Measurements And Transcriptomics Of Metabolic Reprogramming In Activated Macrophages At Fever Temperature, Kenneth Howard Taylor

Graduate Theses and Dissertations

We investigated the real-time metabolic rates of activated macrophage cell lines derived from mouse, chicken and rainbow trout. Each species showed a different kinetic signature with metabolic rates accelerating under fever conditions specific to each organism. Upon activation mouse macrophages increased their rate of glycolysis early and then a later reprogramming phase occurred with a dramatic decrease in oxidative phosphorylation (OXPHOS) accompanied by a reciprocal second increase in glycolytic rates. Chicken macrophages showed an initial increase in both glycolytic and OXPHOS rates. The late drop OXPHOS rates also occurred in chicken however there was no reprogramming that occurred as glycolytic …

Dean's Research Newsletter, May 2025, Neil W. Schluger

SOM Dean's Research Newsletter

No abstract provided.

‭Prevalence Of Potentially Pathogenic Bacteria In Showers At A Long-Term Nursing Care Facility, Joseph Caleb Kulon

Honors Theses

Pathogenic bacteria on environmental surfaces in nursing homes may contribute to the‬ growing burden of healthcare-associated infections (HAIs), particularly as the U.S. population‬ ages and more individuals transition into long-term care. In this study, 70 swab samples were‬ collected from five community shower rooms at a nursing home over three sampling trips. Five‬ common shower surfaces were sampled: the floor drain, shower bench, faucet/valves, shower‬ curtain, and grab bars. Samples were processed in the UTC CIDC microbiology lab and‬ inoculated onto four selective and differential media: Hardy CHROM MRSA agar (for‬ methicillin-resistant‬‭ Staphylococcus aureus‬‭ [MRSA]‬‭ and‬‭ S. pseudintermedius‬‭ [SIM]), mannitol‬ …

Bacterial Contamination Of Wheelchairs In A Long-Term Rehabilitation Facility, Storm A. Welch

Honors Theses

The presence of bacterial contamination on wheelchair surfaces in long-term care facilities poses a significant risk to infection control and patient safety. This study aimed to assess the prevalence and diversity of bacterial species, including Staphylococcus aureus (SA), methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis (SE), Pseudomonas spp., and enteric bacteria on five key components of wheelchairs at a local long-term care facility. A total of 320 swabs were collected from wheelchair components, including handles for pushing, armrests, seats, wheel handles, and tires. Samples were analyzed using selective culture media, and bacterial species were identified based on colony morphology. The results …

Comparison Of Rt-Qpcr And Rt-Ddpcr On Assessing Model Virus In Wastewater, Wafa Youssfi

Graduate Theses and Dissertations

There is an increasing demand for quantifying viral loads in diverse wastewater systems using polymerase chain reaction (PCR). This study evaluates the performance of two commonly used workflows: reverse transcription quantitative PCR (RT-qPCR) and reverse transcription droplet digital PCR (RT-ddPCR) in wastewater. We compared the two methods by measuring the viral ribonucleic acid (RNA) of a model virus Phi6 in samples collected from various treatment stages at the Westside Wastewater Treatment Facility in Fayetteville, AR. RNA was extracted from real and synthetic wastewater samples and analyzed in parallel using both RT-qPCR and RT-ddPCR. Findings reveal that both methods demonstrated similar …

A Review Of Praziquantel Resistance In Schistosomes And The Future Of Schistosomiasis Control, Gabriela G. Eastham

Senior Honors Theses

Schistosomiasis is a group of both acute and chronic parasitic trematode infections of the genus Schistosoma. Praziquantel (PZQ) has been the drug of choice to treat schistosomiasis for decades. Though it is effective against all three clinically relevant species, heavy reliance on PZQ has led to concerns of schistosome resistance, especially in areas that have implemented this drug in mass drug administration (MDA) programs. Although there is currently insufficient evidence to conclude that schistosomes are becoming resistant to PZQ on a large scale, other control methods are a necessity to best treat and eliminate schistosomiasis. This article summarizes the …

Dirty Dianthus: A Review Of The Microbotryum-Dianthus Plant Pathogen System., Roxanne Kaaren Hayes

Electronic Theses and Dissertations

Microbotryum dianthorum is a species complex of smut fungi that act as biotrophic plant pathogens of Dianthus flowering plants. This group of pathogenic fungi are situated within a larger group of fungi that act as biotrophic plant pathogens, some of which are responsible for extensive crop loss in our agricultural system. These pathogens are distinct from other Microbotryum species in their ability to infect not only a single host, but multiple hosts. Their unique ability to parasitize several plant hosts may unlock potential clues about the potential for emergent infection disease in crop systems. Previous work has established that these …

Healthcare Access And Agency In Material Culture: A Specialized Analysis Of Pharmaceutical Artifacts From Louisville’S West End., Sara Marian (Deurell) Ignacio

Electronic Theses and Dissertations

The American Victorian Period of the 1870s to 1920s was a time of significant transitions in medicine. In Louisville, Kentucky, this period saw an influx of Black healthcare professionals and the founding of Louisville National Medical College, the state’s first medical school open to people of color. A number of these professionals lived and/or practiced in the Beecher Terrace study area, which was excavated between 2017 and 2020. Patent medicine brands and archival records were examined qualitatively in order to understand neighborhood and case study household health concerns and consumer choice. Statistical analyses of the medicine bottles recovered during the …

Sars-Cov-2 Modulates Pla2g2d/Pgd2 Signaling To Manipulate Host Immune Response To Increase Disease Severity., Dante L. Hamiel

Electronic Theses and Dissertations

Background: Previous studies suggested that SARS-CoV-2 infection could cause diverse immune and metabolic dysregulations, although the underlying mechanisms remain poorly understood. In addition, viruses may escape the immune response by modulating cellular metabolism, mitochondria, and protein functions indicating that immune or metabolic components may represent novel targets for anti-viral strategies. However, whether immune and metabolic disorders interact or affect each other waits to be determined. On the other hand, previous investigations identified the critical roles of a cell membrane-bound phospholipase PLA₂G2D and its downstream products PGD₂ in regulating the metabolism and inflammation in respiratory system. More importantly, …

D-Mannose Efficacy In Preventing Recurrent Urinary Tract Infections In Women, Miah Lee

Physician Assistant Scholarly Project Posters

• Evaluate the efficacy, safety, and mechanisms of D-mannose, a natural sugar, in preventing and treating UTIs. Evidence from various studies, including randomized controlled trials and pilot research, indicates that D-mannose may reduce UTI recurrence rates and alleviate acute symptoms with comparable efficacy to standard antibiotics. • Recurrent urinary tract infections (rUTIs) are a significant health concern for women, affecting their quality of life and requiring effective and safe prophylactic and therapeutic interventions. • Daily D-mannose demonstrated a significant reduction in recurrence rates for prophylaxis compared to placebo and nitrofurantoin, with fewer adverse effects. • In acute UTI management, D-mannose-based …

Fc Gamma Receptors Facilitate Antigenic Modulation Of Lilrb4 And Function As Predictive Biomarkers In Acute Monocytic Leukemia, Joshua Morse

Dissertations and Theses (Open Access)

Acute monocytic leukemia (monocytic AML) is a subtype of AML marked by a proliferation of abnormal monoblasts. This subtype represents approximately 10% of AML cases. The prognosis of monocytic AML is poor, with a 5-year survival of ~30%. Most patients are diagnosed at an age when they are unlikely to survive first-line non-targeted cytotoxic chemotherapy as a bridge to hematopoietic stem cell transplant (HSCT). Even patients who achieve remission commonly relapse. This population of patients would greatly benefit from precision-targeted therapies but currently there are none approved for monocytic AML.

Leukocyte immunoglobulin-like receptor B4 (LILRB4) is an immune checkpoint expressed …

Expanding Inducible Epithelial Resistance Against Pneumonia: Novel Virus, Novel Receptor, Michael Longmire

Dissertations and Theses (Open Access)

Pneumonia is the leading infectious cause of death worldwide and presents a continuing risk among immunocompromised patients. Our work has been focused for many years on the phenomenon of inducible epithelial resistance, where activation of the innate immune response in the airway epithelium is necessary and sufficient to produce a pathogen-killing but self-protective environment to prevent severe disease. Inducible resistance is pathogen-agnostic and has been demonstrated against an array of respiratory infections. We achieve this using a combination of pattern recognition receptor agonists – Pam2CSK4 and ODN M362 being the current formulation – which produce synergistic levels of pathogen reduction …

Highly Pathogenic Avian Influenza Outbreak Preparedness In The United States: A Scoping Review, Emma J. Gurley

Honors Theses

In order to gauge preparedness for an outbreak of highly pathogenic avian influenza (H5N1) in the United States, a scoping review of the current literature was conducted. This process involved (1) identifying the research question, (2) identifying studies relevant to the topic, (3) selecting studies for inclusion (4) charting the data, and (5) analyzing and reporting the final data. Ultimately, seventeen studies were deemed appropriate for examination within this scoping review and several key themes were identified. The results emphasize the importance of adopting a One Health approach, the key role that vaccination may well play in preventing the spread …

Factors Influencing Community Assembly Of The Amphibian Microbiome In Rochester, Ny, Thomas R. Minahan

Theses

Amphibians are crucial in wetlands and, by extension, our planet. As predator and prey, amphibians serve as keystone species, vital for the continued equilibrium in their wetland ecosystems. However, the global amphibian population is currently threatened by the spread of a pathogenic fungus known as Batrachochytrium dendrobatidis (Bd). Over the past thirty years, Bd has spread worldwide, threatening hundreds of amphibian species. The environmental factors that influence microbial community assembly in amphibians are key to developing interventions that can protect amphibians from disease. This research examines the abiotic and biotic factors influencing microbial community composition in amphibians of Rochester, New …

Back To The Basics: Extracellular Protein Interaction Networks Of The Human Infant Immune System, Mavis Amity Irwin

Theses

Datamining without an age filter proves challenging, especially when searching for direct data from human infants. Although online databases provide immune interaction networks, they often lack information about the age of data sources, resulting in categorizations as age-unspecified. This limitation underrepresents the physiology of naïve immune systems in infants, particularly since full-term infant immunity transitions to an adult-like phenotype between 24 and 30 months of age. This study aims to reconstruct an age-specific immune interaction network for full-term infants by integrating literature-based evidence with existing online database content. A list of 60 extracellular protein candidates involved in immune responses was …

Hemophagocytic Lymphohistiocytosis Secondary To Disseminated Histoplasmosis., Mackenzie Caruthers, Shaili Kothari, Conor Kelly, Kim M Jordan, Branden Luna

Ambulatory and Primary Care Articles

Hemophagocytic lymphohistiocytosis (HLH) is a rare hyperinflammatory condition resulting in erroneous activation of the immune system. Treatment is directed at the underlying pathology that prompts activation of the immune system and usually includes immunosuppressant therapy, including steroids, etoposide, or rituximab. However, the best treatment for patients with significant infection remains unclear. Few cases of HLH are associated with disseminated histoplasmosis, and there are no clear treatment guidelines in these cases. Due to the significant morbidity and mortality associated with HLH, further investigation is needed to identify the best treatment regimens.

The Effect Of Calorie Restriction On Humoral Responses Towards The Influenza Vaccine, Grayson Kobluk

Celebrating Scholarship and Creativity Day (2018-)

Calorie restriction (CR) - a reduction in caloric intake without malnutrition - has been widely studied for its impact on lifespan and the onset of age-related diseases. Emerging research indicates that CR also influences immune function, though its effects can vary depending on the degree of restriction, duration, and individual characteristics. This study examined how CR effects vaccine-specific antibody responses following influenza vaccination. Male and female mice were maintained on either a control diet or a calorie-restricted diet and vaccinated with an inactivated influenza virus vaccine. Antibody titers were measured 28 days post-vaccination to evaluate the magnitude of the humoral …

The Complete Message Is The Best Message: The Case For Standardizing Wireless Emergency Alerts, Jeannette Sutton, Micki Olson, Hugh Walpole, Lauren B. Cain, Nicholas Waugh, Michele M. Wood

Emergency Preparedness, Homeland Security, and Cybersecurity Faculty Scholarship

Standardized messaging for alerts and warnings has been made possible through the use of the Warning Lexicon and workflow. The effectiveness of this approach to motivate protective action behaviors, in comparison with the current standard of practice, has not yet been tested. Using an experimental design to test the effectiveness of the Warning Lexicon, we tested three message types for five hazards: blackouts, law enforcement incidents, infectious diseases, heavy snow, and chemical releases, and found that messages created using the Warning Lexicon contents and workflow produced better messaging outcomes than the current standard of practice. Specifically, participants who received the …

Pelvic Inflammatory Disease, Children's Mercy Kansas City

Clinical Pathways

No abstract provided.

A Multi-Tiered Approach To Improving Vaccination: Adjuvanticity, Alternate Routes Of Delivery, And Science Communication, Haley Agnes Bridgewater

Boise State University Theses and Dissertations

Vaccines have been used to prevent infectious disease for over 200 years. While they are an incredible way to prevent loss of life, they can be improved upon. Researchers around the globe are working to improve the immune response and ultimately the protection provided by existing and new vaccines. In this dissertation, I investigate three ways to improve vaccine immune responses: using an adjuvant, using a prime-boost intramammary delivery route in cows, and using microneedle delivery route. Cholera toxin (CT) is a bacterial toxin that has a non-toxic B subunit (CTB) used as a vaccine adjuvant. However, little is known …

Phosphatase Activity And Structure Of The Spoiie Protein In Clostridioides Difficile, Kathryn Wootten

Biological Sciences Undergraduate Honors Theses

Clostridioides difficile is a major cause of severe gastrointestinal infections and diseases, particularly in healthcare settings. Because of its ability to produce infectious endospores by sporulation, the protein SpoIIE, which is known to play a role in the regulation of this process, is being explored as a potential therapeutic target. In order to gain better insight into the structure and function of this protein, a concentrated and purified sample of the protein must be obtained. The goal of this study was to determine the conditions that would yield the highest amount of SpoIIE. The density of the cultures of bacteria …

The Impact Of Stigma On Individuals Affected By Leprosy: A Pilot Study, Mardo Tarr

Capstone Experience: Master of Public Health

Background: Leprosy, while a treatable condition, continues to be associated with significant stigma, which adversely influences the physical, psychological, social, and economic well-being of affected individuals throughout their lives. The stigma linked to leprosy leads to social exclusion, mental health difficulties, and obstacles to accessing healthcare and employment opportunities (Brakel et al., 2019; Sermrittirong & Van Brakel, 2014). This research employs Life Course Theory to describe the long-term experiences of stigma, coping strategies, and social support individuals living with leprosy. By synthesizing findings from existing literature and conducting a qualitative interview, the study offers an in-depth understanding of how various …

Restoring Trust In Public Health: An Analysis Of The Lasting Implications Of The Covid-19 Pandemic Response On Community Trust In Public Health In Nebraska, Kerri Dittrich

Capstone Experience: Master of Public Health

Background and Significance

Public trust is essential for effective public health efforts. In Nebraska, as in many regions, the pandemic response exposed strengths and vulnerabilities in the public health system. Misinformation, political discourse, inconsistent messaging, and poor communication contributed to growing mistrust, threatening the long-term success of public health programs and initiatives. While national trends have been studied, Nebraska-specific research remains limited.

Methods

An original descriptive survey was designed and administered to public health professionals in Nebraska to assess their perceptions of public trust in the local public health system before and after the COVID-19 pandemic. The survey also explored …