Digital Commons Network^™

Blurred Vision: Understanding Barriers To Initiating Collaborative Market Driving, Kim Feddema, Daniel Schepis, Liudmila Tarabashkina, Sharon Purchase

Research outputs 2022 to 2026

Purpose: This paper aims to examine why many producers of origin-bound products struggle to collectively drive increases in market value, despite shared interests. The authors apply a collaborative market driving (CMD) lens to the early stages of collective efforts, to identify the key motivators, visioning processes and explore why CMD can fail to initiate. Design/methodology/approach: The authors present a qualitative case study of the Western Australian monofloral honey industry. Data includes 64 producers’ and distributors’ online content and 30 interviews with stakeholders. Leximancer was used to map marketing communications, and participant narratives were thematically analysed using terroir and authenticity frameworks. …

Behavioral Responses To Underwater Noise, Christine Erbe, Capri Jolliffe, Renee Schoeman, Valeria Senigaglia, Cristina Tollefsen, Rebecca Wellard, Ann Bowles, Dorian Houser, Shyam Madhusudhana, Sarah A. Marley, Angela Recalde Salas, Chandra Salgado-Kent, Leah Trigg

Research outputs 2022 to 2026

This chapter presents an overview of physical and acoustic behavioral responses of marine mammals to underwater sounds. A literature review was undertaken, and data on received levels at the animal when certain types of responses were observed were compiled in an online supplementary spreadsheet. Based on this, an overview of responses was written, organized first by species and then by sound type. In-air and underwater sound sources were considered. The most studied sound types were mid-frequency sonar and acoustic deterrent devices and then impact pile driving and vessels. The most frequently reported response was avoidance, followed by changes in swim …

Innovation Through Conceptual Research: Tips For Stoking Curiosity, Jun Wen

Research outputs 2022 to 2026

Conceptual research represents a critical step in the theory-building process and contributes innovative ideas that inspire empirical studies. This feature article highlights the importance of such research in academia with examples drawn from the tourism literature. It also critically discusses issues related to publishing conceptual research in premier journals. By using a five-dimension framework of curiosity (i.e., deprivation sensitivity, joyous exploration, social curiosity, stress tolerance, and thrill seeking), scholars can assess researchers’ conceptual topics and readers’ responses when contemplating “what's new” to gauge a paper's publication potential. Several tips are offered to guide like-minded scholars in conducting sound conceptual research: …

The Careful Project: Evaluating The Acceptability Of Local Virtual Reality Experiences As A Leisure Activity For Residents In Aged Care, Davina Porock, Patricia Cain, Christopher Young, Julieanne Hilbers, Maria Bomm, Manonita Ghosh, Mandy Stanley

Research outputs 2022 to 2026

Objectives: The aim of this project was to evaluate the feasibility and acceptability of virtual reality (VR) as a leisure activity for people living in residential aged care. Virtual reality experiences may offer alternatives for residents with limited mobility, or during times when older people are unable to leave residential care. Methods: The intervention used VR videos delivered via a head-mounted device. Video content was created by a local artist specifically for use with older Western Australian adults and used local content. VR sessions were videotaped for analysis. The evaluation included structured observation of video content to assess mood and …

Kaartdijin Bidi (Learning Journey): Place-Based Cultural Regeneration At University, Sandra Wooltorton, Lauren Stephenson, Kathie Ardzejewska, Len Collard

Research outputs 2022 to 2026

Imagine being in a university that functions in a place-based culturally regenerative way. In this concept paper, the authors bring together theory, practice, and experience, in the service of transforming universities towards place-based cultural regeneration. At present, Australian universities operate using an economic philosophy of neoliberal corporatism characterised by hierarchical management strategies, competitive tendencies, patriarchal values, and discourse characterised by bifurcation or binary thinking. These features illustrate a worldview that is entangled with the meta crises of our times such as climate change, species loss, hatred/intolerance, and unfathomable violence. The authors consider ways of moving towards a place-based, Indigenous-informed, practical, …

Review Of Applications Of Deep Learning In Veterinary Diagnostics And Animal Health, Sam Xiao, Navneet K. Dhand, Zhiyong Wang, Kun Hu, Peter C. Thomson, John K. House, Mehar S. Khatkar

Research outputs 2022 to 2026

Deep learning (DL), a subfield of artificial intelligence (AI), involves the development of algorithms and models that simulate the problem-solving capabilities of the human mind. Sophisticated AI technology has garnered significant attention in recent years in the domain of veterinary medicine. This review provides a comprehensive overview of the research dedicated to leveraging DL for diagnostic purposes within veterinary medicine. Our systematic review approach followed PRISMA guidelines, focusing on the intersection of DL and veterinary medicine, and identified 422 relevant research articles. After exporting titles and abstracts for screening, we narrowed our selection to 39 primary research articles directly applying …

A Moving Cause: Travel Notes Towards Pilgrimage As Creative Writing Process, Elizabeth Reid Boyd

Research outputs 2022 to 2026

Pilgrimage Studies is a growing field across the arts and humanities, yet pilgrimage writing is not established as a distinct genre. In this article I share my exploration of pilgrimage as a creative writing process and potential genre. As a social scientist and a creative writer, my approach is qualitative and interdisciplinary, drawing upon an on-the-ground social constructivist perspective and definitions of genre as social action. This includes an ethnomethodological account of my practice that distils ten narrative and thematic elements in the pilgrimage writing process. These elements hinge on the leitmotif of pilgrimage writing being ‘a moving cause’, with …

Implementation Of A Coach-Supported Mhealth Intervention For Dementia Prevention In China: A Qualitative Study Among Chinese Participants And Coaches In The Prodemos Trial, Jinxia Zhang, Marieke P. Hoevenaar-Blom, Xuening Jian, Haifeng Hou, Siqi Ge, Carol Brayne, Esmé Eggink, Melanie Hafdi, Mingyue He, Guohua Wang, Wenzhi Wang, Wei Zhang, Yueyi Yu, Yixuan Niu, Jihui Lyu, Libin Song, Wei Wang, Youxin Wang, Eric P. Moll Van Charante, Manshu Song

Research outputs 2022 to 2026

Background Modifiable risk factors have been linked to 45% of dementia cases. Mobile health (mHealth) interventions targeting lifestyle-related risk factors with remote coaching have the potential to reach underserved high-risk populations globally. To date, little is known about the implementation of such interventions in China. Methods Fifty semi-structured interviews were conducted with 14 participants and 11 health coaches involved in the PRODEMOS trial. This trial investigated whether a coach-supported mHealth application intervention can reduce dementia risk in people aged 55–75 years with multiple risk factors. Interviews were conducted three months and 12–18 months into the intervention, focusing on implementation outcomes …

Ecological Resources Of A Heavily Modified And Utilised Temperate Coastal Embayment: Cockburn Sound, Peter J. Mitchell, Daniel E. Yeoh, Kurt N. Krispyn, Claire N. Greenwell, Sorcha Cronin-O’Reilly, Delphine B.H. Chabanne, Glenn A. Hyndes, Danielle Johnston, David V. Fairclough, Claire Wellington, Alan Cottingham, Gary Jackson, Jeffrey V. Norriss, Matias Braccini, Hector Lozano-Montes, Chandra P. Salgado Kent, Erin Clitheroe, Alissa Tate, James W. Penn, Marion Massam, Neil R. Loneragan, James R. Tweedley

Research outputs 2022 to 2026

Coastal environments and their associated biota provide numerous environmental, economic and societal services. Cockburn Sound, a temperate embayment on the lower west coast of Western Australia, is immensely important for the State and adjacent capital city of Perth. However, urbanisation and associated terrestrial and marine development has the potential to threaten this important ecosystem. This study collated published and unpublished data to review the current state of the ecological resources of Cockburn Sound and describe how they have changed over the past century. Post-WWII, the embayment began undergoing pronounced anthropogenic changes that limited oceanic water exchange, increased nutrient load, modified …

Global Patterns And Drivers Of Seagrass Biomass, Net Primary Production And Meadow Structure, Nicole R. Foster, Enric Gomis, Diana I. Montemayor, Simone Strydom, Miguel Angel Mateo, Eduard Serrano, Aurora M. Ricart, Martin Dahl, Ines Mazarrasa, Paulina Cisternas, Harry Whiteley, Elliott J. Bates, Viena Puigcorbé, Uxue Moreda, Mar Truc, Karina Inostroza, Rafael Huertas, Roisin Mccallum, Anna Lafratta, Chanelle L. Webster, Caitlyn M. O'Dea, Nicole E. Said, Carlos M. Duarte, Paul S. Lavery, Oscar Serrano

Research outputs 2022 to 2026

Aim: We decipher global patterns in seagrass growth and structure to advance seagrass science, facilitate multidisciplinary research, and promote effective management strategies and policy development for seagrass habitats. Location: Global. Time period: 1975 to 2024. Major taxa studied: Seagrasses—marine angiosperms. Methods: A global data set on seagrass total biomass, total net primary productivity (NPP), shoot density, and percent cover was compiled across 11 genera. We tested how these variables change across seagrass genera, life-history strategy, and geographic location; and then modelled the environmental drivers (temperature, nutrients, light availability) of the patterns observed. Results: The mean (±SE) total biomass of seagrasses …

Indigenous Wisdom For Regeneration: Reducing The Knowledge Gap, Sandra Wooltorton

Research outputs 2022 to 2026

The author of this paper uses Indigenous-informed literature and explores the use of a Multispecies Collaboratory to hear place-based voices and practice ways of knowing often denied value by the mainstream. In the Indigenous nation of Australia, the author sets out to learn ways to practice environmental education that build upon aeons of experience at caring for and responding to Country. The starting point is a 2024 paper with three claims, which are: a culture of epistemic violence and denial support the mainstream paradigm which silences Indigenous voices; an Indigenous-informed way of being with living-places can inform the core of …

Nuclear Destabilisation – A Possible Genesis Of Cancer?, Daniel D. Scott, Francesco Bettariga, Marco Ventin, Chris Bishop, Britta Stordal

Research outputs 2022 to 2026

This review examines the increasingly prominent role of mechanics within cancer formation and progression. The extremely varied and contradictory genetic landscape of cancer is in stark contrast to the seemingly universal mechanical characteristics of cancer cells and their tumour microenvironment, and mechanics may be a principal unifying trait of this disease. The tight regulation of innate cell mechanical properties raises the possibility that destabilisation of the cell drives tumour formation in an attempt to restore cell mechanical homeostasis. With losses in cell stiffness more pronounced at the cell nucleus, we hypothesise that destabilisation occurs within the nucleus, likely within the …

Varying Vulnerabilities: Seagrass Species Under Threat From Prolonged Ocean Warming, Marnie L. Campbell, Chi T.U. Le

Research outputs 2022 to 2026

Understanding the response of various seagrass species to prolonged elevated water temperatures is crucial for effective management and seagrass species restoration amid increasing climate change-induced ocean warming and marine heat waves. This is especially important in intertidal seagrass meadows, where heat can penetrate substrate depths of up to 50 cm. We assessed and contrasted the responses of five intertidal species—Zostera muelleri Irmisch ex Asch, Halophila ovalis (R.Br.) Hook f., Halodule uninervis (Forssk.) Asch, Halophila decipiens Ostenf., and Halophila spinulosa (R.Br.) Asch—over a month of elevated temperatures by examining rhizome growth, number of living shoots, root development, and sprig survival. While …

Volumetric Comparison Of Overall Brain And Neuropil Size Between Social And Non-Social Spiders: Exploring The Social Brain Hypothesis, Vanessa Penna-Gonçalves, Donald James Mclean, Nikolas J. Willmott, Michael B.J. Kelly, Jay R. Black, Elizabeth C. Lowe, Marie E. Herberstein

Research outputs 2022 to 2026

The social brain hypothesis predicts that the relative size of specific brain regions is driven by the cognitive capacity required to manage complex (social) situations. Spiders are intriguing models to test this hypothesis, as sociality is rare in this usually solitary and aggressive group. Here, we used microCT to compare the central nervous system and brain volumes between social and solitary females of the species in two taxonomic groups, huntsman and crab spiders. Overall, we found no difference in relative CNS and brain volume between social and solitary species. However, social huntsman spiders Delena cancerides had larger arcuate and mushroom …

Techno-Economic And Eco-Friendly Msw-Based Strategy To Reduce The Impact Of Rajshahi City Electric Vehicle Power Outage In Bangladesh, Shah Tanvir Alam Rimon, Md Shahriar Mohtasim, Md Golam Kibria, Mim Mashrur Ahmed, Barun K. Das

Research outputs 2022 to 2026

Population growth, economic expansion, urbanization, and evolving consumer habits are driving a significant increase in municipal solid waste generation, placing Bangladesh under immense societal pressure to manage and dispose of this waste effectively. Numerous studies have examined municipal solid waste management practices in Bangladesh, yet the potential for utilizing the generated energy to charge electric vehicles (EVs) remains unexplored. Therefore, in this study, waste-to-energy technologies such as landfill gas recovery (LGR), incineration (INC) and anaerobic digestion (AD) were assessed for energy recovery, economic feasibility, and global warming potential in Rajshahi City Corporation, Bangladesh, to meet the extra electricity demand imposed …

Circulating Lipocalin-2 Across The Adult Lifespan, Carlie Bauer, Cassandra Smith, Sara Vogrin, Andrew S. Palmer, Mary Woessner, Shanie Landen, Macsue Jacques, Elizabeth Byrnes, Nir Eynon, Marc Sim, Joshua R. Lewis, Itamar Levinger

Research outputs 2022 to 2026

Lipocalin-2 (LCN2), a hormone produced by adipocytes, osteoblasts and renal tubular cells, is implicated in age-related diseases, including cardio-metabolic disease. To understand the role LCN2 may play in pathological states, we first need to elucidate the relationship between circulating LCN2 with indices of cardio-metabolic health during “normal” ageing. This study examined the relationship between serum levels of LCN2, age and cardio-metabolic measures across the adult lifespan in males and females. We conducted a pooled cohort analysis including 124 community-dwelling males (n = 52) and females (n = 72) (age 20 - 87 years, median BMI 25.92 (23.04, 29.81) kg/m2). Serum …

Value Incoherence Precedes Value Change: Evidence From Value Development In Childhood And Adolescence Across Cultures, Ella Daniel, Anat Bardi, Julie A. Lee, Ricarda Scholz-Kuhn, Einat Elizarov, Jan Cieciuch, Ariel Knafo-Noam, Alice Ramos, Michele Vecchione, Rene Algesheimer, Evelia Murcia Alvarez, Avital Ben Dror Lankry, Maya Benish-Weisman, Ricardo Borges Rodriques, Anat Chomsky, Patricia R. Collins, Eldad Davidov, Anna K. Doring, Stefanie Habermann, Dana Katsoty, Martin Kindschi, Elena Makarova, Gilda Marsicano, Kinneret Misgav, Thomas P. Oeschger, Leonor Pereira Da Costa, Joanne Sneddon, Iva Tendais, Louise Twito-Weingarten

Research outputs 2022 to 2026

We test the theory that personality incoherence may instigate personality change in the context of personal values. Values’ near-universal organization makes value incoherence assessment straightforward. The study included 13 longitudinal samples from seven cultures (Australia, Israel Palestinian citizens, Israel Jewish majority, Italy, Poland, Portugal, and Switzerland), total N = 7,126, and T1 Mage ranging between 6 and 18. Each participant reported values between two- and six-times. Using unfolding analysis, we calculated the fit of the internal value structure of each participant at the first time point to the value structure in their sample (normative structure) and to the theoretical structure …

Transforming Transitions Ii: Building Children's Engagement In Learning At School, Lennie Barblett, Fiona Boylan, Amelia Ruscoe

Research outputs 2022 to 2026

Building on the success of the Transforming Transitions project conducted with ten Western Australian Independent Schools (Barblett et al., 2023), this study extended the inquiry into school engagement and the transition process. The project was reimagined to deepen collaboration with schools, focusing on two key areas: first, fostering meaningful engagement with young learners and their families during the transition to school by drawing on their funds of knowledge; and second, centering the perspectives of children as active participants in their transition, acknowledging their experiences and the role of family-school engagement in shaping their sense of belonging.

Comparing Microct Staining And Scanning Methodology For Brain Studies In Various Sizes Of Spiders, Vanessa Penna-Gonçalves, Nikolas J. Willmott, Michael B.J. Kelly, Jay R. Black, Elizabeth C. Lowe, Marie E. Herberstein

Research outputs 2022 to 2026

Recent advances in microCT are facilitating the investigation of microstructures in spiders and insects leading to an increased number of studies investigating their neuroanatomy. Although microCT is a powerful tool, its effectiveness depends on appropriate tissue preparation and scan settings, particularly for soft, non-sclerotized tissues, such as muscles, organs, and neural tissues. As the application of microCT in spiders is only in its infancy, published protocols are often difficult to implement due to substantial size variation of the specimens. The present study was initiated to determine how to account for this variation. Our work builds on previous methods using microCT …

Diverged Populations Admixture Bolsters Genetic Diversity Of A New Island Dibbler (Parantechinus Apicalis) Population, But Does Not Prevent Subsequent Loss Of Genetic Variation, Rujiporn Thavornkanlapachai, Harriet R. Mills, Kym Ottewell, Cathy Lambert, J. Anthony Friend, Daniel J. White, Zahra Aisya, W. Jason Kennington

Research outputs 2022 to 2026

Translocating individuals from multiple source populations is one way to bolster genetic variation and avoid inbreeding in newly established populations. However, mixing isolated populations, especially from islands, can potentially lead to outbreeding depression and/or assortative mating, which may limit interbreeding between source populations. Here, we investigated genetic consequences of mixing individuals from two island populations of the dibbler (Parantechinus apicalis) in an island translocation. Despite a high level of genetic divergence between the source populations (FST ranges 0.33–0.64), and significant differences in body size, individuals with different ancestries were able to successfully interbreed in captivity and in the wild. However, …

Exploring The Relationship Between Melanopsin Gene Variants, Sleep, And Markers Of Brain Health, Ayeisha Milligan Armstrong, Eleanor O'Brien, Tenielle Porter, Vincent Dore, Pierrick Bourgeat, Paul Maruff, Christopher C. Rowe, Victor L. Villemagne, Stephanie R. Rainey-Smith, Simon M. Laws

Research outputs 2022 to 2026

INTRODUCTION: Melanopsin is a photopigment with roles in mediating sleep and circadian-related processes, which are often disrupted in Alzheimer's disease (AD). Melanopsin also impacts cognition and synaptogenesis. This study investigated the associations between melanopsin genetic variants, sleep, and markers of brain health. METHODS: Linear regression analyses examined the relationship of single-nucleotide polymorphisms (SNPs) within the melanopsin gene (OPN4), with cortical amyloid beta (Aβ), cognition, brain volumes, and self-reported sleep traits in cognitively unimpaired older adults. Further analyses assessed whether sleep traits x OPN4 SNP interactions were associated with markers of brain health. RESULTS: OPN4 SNPs rs2355009 and rs3740334 were associated …

Goldenbraid20 E Coli: A Comprehensive And Characterized Toolkit For Enterics, Matthew B Cooke, Kobie T Welch, Laura D Ramirez, Alice X Wen, David C Marciano, Christophe Herman

Faculty, Staff and Students Publications

Modular cloning systems streamline laboratory workflows by consolidating genetic 'parts' into reusable and modular collections, enabling researchers to fast-track strain construction. The GoldenBraid 2.0 modular cloning system utilizes the cutting property of type IIS restriction enzymes to create defined genetic 'grammars', which facilitate the reuse of standardized genetic parts and assembly of genetic parts in the right order. Here, we present a GoldenBraid 2.0 toolkit of genetic parts designed to accelerate cloning in the model bacterium

Seeing, Feeling, And Hearing The World. A Regenerative Worldview: Rinyi, Pirlirr And Liyan, Kankawa Nagarra Knight, Anne Poelina, Sandra Wooltorton

Research outputs 2022 to 2026

In this communication, the Australian authors-two Indigenous women and one woman with Anglo-Celtic ancestry-take us into Western Australian Indigenous language and worldviews, to help us reach toward a regenerative worldview. Indigenous words such as rinyi, pirlirr, and liyan are explored to point us in a direction unfamiliar to many English speakers, to Land and Country as living and responsive. The authors notice that it is very difficult to describe these terms in English, because English language does not seem sufficiently capacious to describe the depth of relational being-with Country that Indigenous languages portray. This may be changing, as various Indigenous …

Detection Of Vkorc1 Single Nucleotide Polymorphisms Indicates The Presence Of Anticoagulant Rodenticide Resistance In Australia's Introduced Rats, Alicia F. Gorbould, Quinton F. Burnham, Michael T. Lohr, Annette Koenders

Research outputs 2022 to 2026

BACKGROUND: Anticoagulant rodenticides (ARs) are used globally to manage pest rodent populations. However, resistance to ARs in target rodent populations challenges pest control efforts and can increase risks to nontarget species. Resistance is frequently associated with nonsynonymous single nucleotide polymorphisms (nsSNPs) in the Vkorc1 gene, and this study carried out the first Vkorc1 survey of introduced rats on the Australian mainland. RESULTS: We identified three species of introduced rat using the cytochrome b gene across Brisbane, Melbourne, Perth and Sydney: Rattus rattus (Linnaeus 1758) (Lineage I); Rattus norvegicus (Berkenhout 1769); and Rattus tanezumi (Temminck 1844) (Lineage II). Three nsSNPs were …

Possibilities For Climate Change Adaptation Investments In Ghana: Exploring Private Sector Concerns And Perceptions About Policy Incentives, Jane Osei, Albert Ahenkan, Samuel Weniga Anuga

Research outputs 2022 to 2026

Policy incentives can significantly boost private sector investment in climate change adaptation, especially in developing countries. Leveraging private sector resources, including human capital, financial assets, and technological innovations, is crucial for enhancing adaptation efforts. However, many private entities are reluctant to invest due to the perceived business risks associated with climate change. This hesitancy increases vulnerability in developing nations. This study examines the private sector's views in Ghana on policy incentives for climate adaptation investments and the barriers impeding their involvement. The findings show that corporate social responsibility, financial requirements, and access to relevant information are key motivators for private …

Belongingness, Trust And Connection: Arts Teachers In Western Australian Schools Create Safe Learning Spaces, Christina Gray, Lisa Paris, Geoffrey Lowe, Angela Perry, Lara Warwick

Research outputs 2022 to 2026

Central to this phenomenological inquiry is the question of how students’ fundamental needs of belonging, trust and connection are enacted in arts classrooms. Through qualitative semi-structured interviews, this research explores the teaching practices of 23 expert arts teachers in Western Australian schools, revealing strategies to sustain supportive and safe learning environments. The findings highlight the disconnection students face in their schools and the significance of trust in embracing diversity, cultivating quality relationships, and empowering student voice. Understanding these evolving practices can enable less experienced teachers to adapt arts-based approaches to foster inclusive spaces that prioritise student well-being and connection.

Children’S ‘Playbour’ As Influencers On Social Media: An Investigation Into The Legal And Ethical Issues Surrounding Kidfluencers, Catherine J. Archer, Kate Mirandilla Delmo

Research outputs 2022 to 2026

The legal and ethical concerns around children performing as social media influencers (SMIs) on various social media platforms have been in the spotlight, with the recent world-first decision by the Australian Government to ban social media for those under 16. Social media influence is big business across the globe, with organisations investing in working with influencers to promote their brands and/or ideas. Some children now ‘work’, often managed by their parents, to produce posts that promote brands and ideas on behalf of organisations–not only to other children but also to adults. This paper presents findings of two case studies of …

Joint Channel Allocation And Power Control For Mission-Critical Applications In Sustainable Underwater Acoustic Networks, Sadaf Vahabli, Iftekhar Ahmad, Quoc Viet Phung, Daryoush Habibi

Research outputs 2022 to 2026

Underwater Acoustic Networks (UANs) are gaining popularity for underwater communication due to their long-range capabilities. However, they experience significant challenges, including limited bandwidth and spectrum overlap with marine mammal vocalizations, which raises concerns about the sustainable and ethical use of underwater acoustic resources. This study proposes an eco-friendly approach that integrates joint channel allocation and power control to minimize harmful interference with marine life while ensuring reliable communication for mission-critical applications, such as diver safety. To balance marine protection and communication reliability, this work introduces a strategy for intelligent spectrum management, where nodes first sense the acoustic spectrum to detect …

Learning-Associated Astrocyte Ensembles Regulate Memory Recall, Michael R Williamson, Wookbong Kwon, Junsung Woo, Yeunjung Ko, Ehson Maleki, Kwanha Yu, Sanjana Murali, Debosmita Sardar, Benjamin Deneen

Faculty, Staff and Students Publications

The physical manifestations of memory formation and recall are fundamental questions that remain unresolved1. At the cellular level, ensembles of neurons called engrams are activated by learning events and control memory recall1-5. Astrocytes are in close proximity to neurons and engage in a range of activities that support neurotransmission and circuit plasticity6-10. Moreover, astrocytes exhibit experience-dependent plasticity11-13; however whether specific ensembles of astrocytes participate in memory recall remains obscure. Here we show that learning events induce c-Fos expression in a subset of hippocampal astrocytes, which subsequently …

Early Cerebrospinal Fluid Elevations Of Ptau-217 In Severe Traumatic Brain Injury Subjects, Hamad Yadikar, Firas H Kobeissy, Claudia Robertson, Spyridoula Tsetsou, John B Williamson, Damon G Lamb, Amy K Wagner, Todd Kibaugh, Shih-Han Kao, Zhifeng Kou, Robert D Welch, Jose-Miguel Yamal, Luis Leon-Novelo, Richard Rubenstein, Kevin K W Wang

Faculty, Staff and Students Publications

Introduction: Tauopathies, including Alzheimer's disease (AD), feature abnormal accumulations of hyperphosphorylated Tau protein; however, their biomarker potential in traumatic brain injury (TBI) is not well-defined. This study investigated whether cerebrospinal fluid (CSF) phosphorylated Tau at threonine-217 (pTau-217) could serve as an early biomarker for severe TBI (sTBI).

Methods: CSF samples from 26 sTBI patients, collected between 6 and 240 h post-injury, and 19 healthy controls were analyzed using an optimized direct enzyme-linked immunosorbent assay (ELISA; sensitivity < 4.7 pg/mL) for pTau-217 detection, complemented by Western blot validation. Temporal analysis, ROC curves, and trajectory clustering were used for interpretation.

Results: CSF pTau-217 levels were significantly elevated in sTBI patients at 6, 12, 18, 24, and 48 h post-injury compared to controls (p …