Digital Commons Network^™

Fusobacterium Sphaericum Sp Nov, Isolated From A Human Colon Tumor Adheres To Colonic Epithelial Cells And Induces Il-8 Secretion, Martha A Zepeda-Rivera, Yannick Eisele, Alexander Baryiames, Hanrui Wu, Claudia Mengoni, Gianmarco Piccinno, Elsa F Mcmahon, Kaitlyn D Lacourse, Dakota S Jones, Hans Hauner, Samuel S Minot, Nicola Segata, Floyd E Dewhirst, Christopher D Johnston, Susan Bullman

Faculty, Staff and Student Publications

Cancerous tissue is a largely unexplored microbial niche that provides a unique environment for the colonization and growth of specific bacterial communities, and with it, the opportunity to identify novel bacterial species. Here, we report distinct features of a novel Fusobacterium species, F. sphaericum sp. nov. (Fs), isolated from primary colon adenocarcinoma tissue. We acquire the complete closed genome and associated methylome of this organism and phylogenetically confirm its classification into the Fusobacterium genus, with F. perfoetens as its closest neighbor. Fs is phenotypically and genetically distinct, with morphological analysis revealing its coccoid shape, that while similar to …

A Review Of Engraftment Assessments Following Fecal Microbiota Transplant, Chloe Herman, Bridget M Barker, Thais F Bartelli, Vidhi Chandra, Rosa Krajmalnik-Brown, Mary Jewell, Le Li, Chen Liao, Florencia Mcallister, Khemlal Nirmalkar, Joao B Xavier, J Gregory Caporaso

Faculty, Staff and Student Publications

Fecal Microbiota Transplant (FMT) is a treatment for recurrent Clostridium difficile infections and is being explored for other clinical applications, from alleviating digestive and neurological disorders, to restoring microbiomes impacted by cancer treatment. Quantifying the extent of engraftment following an FMT is important in understanding a recipient's response to treatment. Engraftment and clinical response need to be investigated independently to evaluate an FMT's role (or lack thereof) in achieving a clinical response. Standardized bioinformatics methodologies for quantifying engraftment extent would not only improve assessment and understanding of FMT outcomes, but also facilitate comparison of FMT results and protocols across studies. …

Effects Of Urbanization On The Population Genetics And Morphology Of The White-Footed Mouse (Peromyscus Leucopus) Across An Urban–Rural Gradient, Sydney A. Morton

Master's Theses

Urbanization alters landscapes in ways that can reshape wildlife populations by changing habitat connectivity, resource availability, and selective pressures. To investigate how urbanization influences both genetic differentiation and morphology in small mammals, I examined Peromyscus leucopus (PELE) across an urban–rural gradient in northern Georgia. Using RADseq SNP data from PELE individuals across 13 sites, I quantified pairwise genetic differentiation (F_ST) and tested how various environmental variables, including impervious surface cover, human population density, and tree canopy cover, predict genetic differentiation after accounting for geographic distance. Genetic differentiation increased with geographic distance and, after controlling for isolation by …

Raise Your Digital Hand – Multilingual Learner Student And Teacher Perceptions Of Live Digital Feedback, Naveed Khan

Dissertations

Teachers of Multilingual Learners (MLs) must support students with varying English proficiency as they work toward meaningful academic progress. One promising support is live digital feedback, which can be multimodal—written, typed, drawn, or audiovisual—and serves as an added scaffold as MLs work within their zone of proximal development (ZPD). This qualitative case study examined ML student and teacher perceptions of live teacher and peer digital feedback in one-to-one (1:1) classrooms, grounded in sociocultural theory, effective feedback, and multimodality. The research questions focused on the types of feedback provided by teachers and peers and how ML students—both recipients and peer helpers—and …

Establishing Procedures For Primary Human Myoblast Cell Culture, Erica Duffy

Biomedical Engineering

Peripheral Artery Disease (PAD) affects an estimated 200 million people worldwide and is the leading cause of amputation in the United States. PAD is characterized by atherosclerotic narrowing of arteries, resulting in ischemia from reduced blood flow to distal tissues. Although surgical and percutaneous interventions can restore perfusion, fewer than 50% of patients experience sustained benefit, highlighting the need for alternative therapeutic strategies. One promising approach is promoting arteriogenesis, the growth and outward remodeling of pre-existing collateral vessels that improve blood flow around the occluded arteries. Arteriogenesis is regulated by regenerative, M2-polarized macrophages, making macrophage polarization a key therapeutic target. …

Environmental Identities Of College Students Reveal Potential Conflicts And Common Ground For Wildlife Conservation, Richard Von Furstenberg, Lincoln R. Larson, M. Nils Peterson, Kangjae Jerry Lee, Victoria R. Vayer, Kathryn Stevenson, Stacy A.C. Nelson, Jeremy Bruskotter, Adam A. Ahlers, Christine Anhalt-Depies, Taniya Bethke, Chris Chizinski, Brian Clark, Kiley M.D. Fryman, Ashley A. Dayer, Benjamin Ghasemi, Larry Gigliotti, Alan Graefe, Samuel J. Keith, Matt Kelly, Gerard Kyle, Elizabeth Metcalf, Wayde Morse, Mark D. Needham, Neelam C. Poudyal, Michael Quartuch, Shari L. Rodriguez, Chelsie Romulo, Ryan L. Sharp, William Siemer, Matt Springer

Michigan Tech Publications

“Conservationist” and “environmentalist” are two prominent environmental social identities often perceived as conflicting, particularly on wedge issues like hunting. While these groups may hold differing philosophies, their beliefs could overlap, revealing opportunities for collaboration in conservation. We examined environmental identities among U.S. college students across 22 states (n = 17,203) from 2018 to 2020, assessing identity, wildlife values, and positions on polarizing issues. Students were classified into four groups: conservationists (8 %), environmentalists (9 %), pluralists (59 %) who expressed both identities, and eco-agnostics (24 %) who expressed neither. Environmentalists, the most diverse group demographically, exhibited mutualistic wildlife value orientations, …

Occurrence Of Per And Polyfluoroalkyl Substances In Rural Wastewater Treatment Plants And Comparison To Large Treatments Plants, Madeleine Rauhauser

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Per and polyfluoroalkyl substances (PFAS) are a class of compounds defined by their carbon – fluorine bonds, which are incredibly strong. The strength of this bond makes these compounds very useful industrially, they are used as waterproof and grease proof coatings, and as fire retardants. The same thing that makes them useful industrially makes them incredibly recalcitrant in the environment, it is difficult to destroy these compounds. PFAS are also suspected to cause health impacts in people, such as kidney cancer and low birth weights. Wastewater has been commonly identified as a contributor to the amount of PFAS in the …

Detecting Prescribed Fire, Haying And Grazing Events Via Remote Sensing To Create Grassland Disturbance Landcovers For The Ring-Necked Pheasant (Phasianus Colchicus), Megan Amy Baldissara

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation developed disturbance detection models to fulfill the need for remote sensing landcover products describing grassland structure. The use of landcover products derived from remote sensing is increasing over time in pheasant (Phasianus colchicus) research. Such landcover, however, does not provide relevant pheasant structural habitat information (Chapter 1). Pheasants require tall, high-density grassland for nesting, tall grassland with medium density for brood rearing, and tall grassland for wintering. Time since disturbance can serve as a proxy for structure, as it shapes vegetation by removing biomass and resetting succession. Disturbance is easier to detect than structure with current …

Advanced Membrane Methods For Treatment Of Toxic Contaminants And Education Of Early-Career Researchers, Thomas Mckean Iii

Graduate Theses and Dissertations

Membrane technologies are attractive as they are linearly scalable and often require lower operating costs compared to other technologies. One area where they are particularly effective is the treatment of water contaminated with toxic substances. The simplicity of this removal mechanism provides reliable performance capable of meeting stringent removal requirements. However, membrane fouling is a major challenge that often limits their commercial viability. This research sought to investigate novel approaches to pretreatment and membrane modification to overcome fouling and high operating pressures. In addition, membrane separation processes can often be developed into simple yet educational experiments that are ideal for …

National Hero And Water Deity: The Worship Of Triệu Việt Vương In The Red River Delta, Vietnam, Huong Thi Nguyen

Graduate Theses and Dissertations

In my dissertation, the theory of collective memory will be deployed to address many questions regarding retaining, recovering, and re-creating the worship of King Triệu Việt Vương. He was a legendary, historical king of Vietnam who supposedly lived in the 6th century. His achievements were connected to the people living in the Red River Delta in their fight against the Liang invasion. The villagers who lived near or in this area also honored him for his spiritual power. They built a small temple/ shrine right by the riverbank to worship this King. The Vietnamese dynasties, Ly (in the 11th century), …

World On Fire: The Hunger Games And The Rhetoric Of Revolution, Rachel Olivares

Electronic Theses and Dissertations

In the decade between 2010 and 2020, more people participated in mass protests than at any other point in human history. From the Arab Spring to Occupy Wall Street and Hong Kong’s “umbrella movement,” street demonstrations exploded on a global scale. This period of global unrest coincided with another explosion: the dystopian young adult series The Hunger Games surged in popularity, quickly becoming a cultural phenomenon. Following the release of the book series and its subsequent film adaptations, The Hunger Games became a global symbol of grassroots resistance as social and political movements adopted the language and symbols of its …

The Influence Of Tert-Butylhydroquinone (Tbhq) On Acod1/Irg1-Driven Inflammatory Pathways, Alyssa M. Whisel

All Dissertations

The demand for processed foods has led to increased use of synthetic phenolic antioxidants (SPAs), notably tert-butylhydroquinone (TBHQ), to inhibit lipid oxidation and prolong shelf life. While TBHQ is deemed safe at regulated levels, concerns arise from studies indicating potential adverse effects such as oxidative stress, genotoxicity, and impaired immune function. To investigate TBHQ's immunomodulatory effects, RAW 264.7 murine macrophages were pre-treated with TBHQ and subsequently stimulated with lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly I:C, PIC) to simulate bacterial or viral challenges. Results demonstrated that TBHQ at high non-cytotoxic doses reduced nitric oxide and IL-10 production while promoting phagocytosis and …

Drivers Of Faunal Communities In Isolated Wetlands: Local And Landscape Determinants Of Assemblages, Joshua Holbrook

All Dissertations

Ecological interactions such as predation are vital to shaping the distribution of organisms. For interactions to occur, organisms must undertake movement, such as dispersal, that places them in proximity to the organisms with which they may interact. Geographically isolated wetlands (GIW) are productive and biodiverse ecosystems, but little is known on broad geographic scales about the determinants of their fauna. Fishes have difficulty colonizing GIW, whereas amphibians and macroinvertebrates are adept overland dispersers – though they may be sensitive to top-down control by fishes. To better understand these phenomena, I sampled 10-20 wetlands each at nine regions in North and …

Digital Reflections: Evaluating Body Dissatisfaction In Xr Through Eye- And Body-Tracked Virtual Humans, Deyrel Diaz

All Dissertations

In an era where digital and physical realities increasingly intertwine, the perception of body image is undergoing a significant transformation. Traditional understandings of body dissatisfaction, long studied in relation to psychological distress and eating disorders, are now being reshaped by technologies such as Virtual Reality (VR), Augmented Reality (AR), and Artificially Intelligent (AI)- generated media. These technologies have introduced novel ways of experiencing and interacting with the human form, raising critical questions about their impact on self-perception and internalization of beauty standards.

As virtual representations become more prevalent in entertainment, social media, and interactive platforms, it is becoming more crucial …

Interfaces To Enhance Performance In Human-Ai Teams Conducting Safety-Critical Military Operations: A Route Reconnaissance Use Case, Sarvesh Sawant

All Dissertations

Military teams frequently operate in hazardous and dynamic environments where they must recognize threats, act swiftly, and maintain awareness of their surroundings. To assist soldiers in identifying threats, analyzing data, and creating safer routes, Artificial Intelligence (AI) systems are being integrated into these teams. But only when humans comprehend what AI is doing, why it is doing it, and how to collaborate with it can AI be helpful in these high-stakes missions. To enhance human-AI collaboration during military route reconnaissance missions, this dissertation explores ways to improve human-AI communication. In a route reconnaissance mission, teams of soldiers must navigate through …

Universal Systems Simulation Via Constraint Hypergraphs With Applications To Digital Twins, John Morris

All Dissertations

The characterization of systems encompasses a variety of modeling frameworks designed to capture specific behaviors and components of various system domains. Whatever the framework, the core elements of a system representation are the information of the system and a description of how that information is related. The relations in deterministic systems are functions, which, when composed to form executable processes, can be used to simulate system data. A declarative modeling framework is one that encodes mechanisms for preparing these simulations within the model structure, allowing an external agent to form the execution processes required for a given context. To date, …

Exploring Glycosomes As Therapeutic Targets For Trypanosoma Brucei, Sabrina Sutton Pizarro

All Dissertations

Trypanosoma brucei is a protozoan parasite which causes human African trypanosomiasis and the livestock wasting disease, nagana. These parasites have highly specialized peroxisomes, called glycosomes, which house a variety of essential metabolic pathways, including glycolysis. Glycolysis is the sole means of energy production in the infective life stage of T. brucei making glycosomes and the glycolytic pathway promising targets for therapeutic strategies. In this work, we have studied the glycosome as a therapeutic target from multiple perspectives. The first portion of this work addresses drug trafficking. The efficacy of small-molecule inhibitors of glycosomal enzymes is restricted by the rate of …

Better Floors, Better Health: A Theory Of Change For An Improved Household Flooring Intervention In Rural Communities In Kwale And Bungoma Counties, Kenya, Stella Kepha, Hugo Legge, Katherine E. Halliday, Victoria Ochwal, Lynne Elson, Jacinta Mwongeli, William E. Oswald, Beatrice Kakoi, James Wambua, Charles Mwandawiro, Ulrike Fillinger, Rachel Pullan, Doris Njomo

All Peer-Reviewed Publications

Background: Household flooring is increasingly being investigated as a determinant of health, however the pathways through which flooring may impact health and wellbeing are not yet well understood. The SABABU study is a cluster-randomised controlled trial evaluating the impact of an improved flooring intervention on soil-transmitted helminthiasis, tungiasis, and enteric infections in Bungoma and Kwale counties, Kenya. This paper presents the findings from a theory of change development process that was undertaken as part of the formative research phase of the SABABU project. Methods: A co-creation workshop (n = 1), stakeholder meetings (n = 2), and community meetings (n = …

Armando’S Case: Diagnostic Emergent Literacy Assessment For Children With Complex Communication Needs, Bronwyn A. Harris, Tonya R. Moore, Ashley E. Pennell

Journal of Case Learning & Exceptional Learners

This practice-based case study is designed to offer a tutorial on how to adapt an informal, diagnostic emergent literacy assessment to meet the needs of students like six-year-old Armando, a Latino, multilingual learner with autism spectrum disorder (ASD) and complex communication needs (CCN) in an inclusive kindergarten classroom. Armando’s informal literacy assessment adaptations and outcomes are discussed, including descriptions of his emergent literacy skills observed through his engagement in literacy stations, small group reading instruction in his classroom, and his use of alternative and augmentative communication (AAC) in both instructional and assessment settings. This case study illustrates how traditional literacy …

Once Upon A Time: A Kinesthetic Approach To Teaching Evidence, Peter C. Alexander, Cheryl T. Page, Hannah G. Chapman

Southern Illinois University Law Journal

This Article describes and evaluates a kinesthetic, simulation-based approach to teaching Evidence at Southern Illinois University Simmons Law School. Departing from the traditional Langdellian, case-method model, one section of Evidence requires students to memorize and apply the Federal Rules of Evidence through a series of five scaffolded mini-trials built around fractured fairy tales and nursery rhymes. Drawing on the MacCrate Report, Bloom’s taxonomy, and Vygotsky’s theory of scaffolding, we situate this pedagogy within the broader movement toward experiential legal education and argue that Evidence—because of its centrality to litigation practice—is an ideal doctrinal course in which to integrate trial advocacy …

The Messenger, Vol. 52, No. 2

The Messenger (archival - 1870-)

No abstract provided.

Diseño Visual, Identidad De Género Y Diversidad Cultural En La Animación Contemporánea: El Caso De Raya And The Last Dragon, Elisabet Fonts González

GDI. Revista de investigación de Género, Diseño e Innovación

En los últimos años, la animación ha evolucionado hacia representaciones más inclusivas de género y diversidad cultural. Raya and the Last Dragon (López Estrada & Hall, 2021) destaca como un caso significativo de esta transformación, integrando elementos visuales, narrativos y simbólicos que reconfiguran los arquetipos femeninos tradicionales. Este estudio adopta un enfoque cualitativo centrado en el análisis visual, narrativo y simbólico de los personajes Raya, Namaari y Sisu. Basándose en fuentes primarias del proceso creativo y marcos teóricos de la animación y los estudios de género (Furniss, 2017; Lauretis, 1987; Wells, 1998), presta especial atención al diseño de personajes, la …

Elena Asins Y Las Afueras Del Canon: Animación Computacional Y Resistencia, Maria Mar Garrido Román

GDI. Revista de investigación de Género, Diseño e Innovación

Este artículo examina las animaciones generadas por ordenador producidas por Elena Asins, analizando cómo su práctica artística moviliza el pensamiento lógico-matemático, la programación y la experimentación visual. A través de una revisión de su metodología, contexto de producción y resultados formales, el estudio explora la incorporación de procesos algorítmicos en su obra, que generan imágenes con extensión temporal y la consolidan como una figura clave en la historia del arte digital y la animación experimental en España.

La investigación se basa en el análisis de fuentes primarias y, con el objetivo de situar su producción en un marco internacional, establece …

Una Animación Propia. Discurso Emancipador Desde El Cine De Animación Independiente, Elisa Martínez, Damià Jordà

GDI. Revista de investigación de Género, Diseño e Innovación

Introducción: A pesar de la temprana incorporación de las mujeres a la industria de la animación, sus roles fueron históricamente relegados a labores mecánicas, limitando su capacidad de decisión creativa. Este artículo analiza cómo el cine de animación independiente del siglo XX funcionó como un espacio esencial para las directoras que buscaban un control artístico que permitiera desarrollar discursos emancipadores. Se parte de la hipótesis de que el circuito independiente propició que las animadoras desarrollaran técnicas y narrativas alternativas que rompieron con los cánones plásticos y los preceptos ideológicos dominantes en la industria. Metodología: Mediante una metodología cualitativa, …

Animación Por Mujeres Colombianas: La Potencia Expresiva Del Trabajo Hecho A Mano, Daniela Posada Sarmiento

GDI. Revista de investigación de Género, Diseño e Innovación

Este artículo busca situar el trabajo de tres mujeres animadoras colombianas dentro del campo de investigación sobre el género y la animación, evidenciando la escasa presencia de sus aportes en la investigación académica. Se pretende demostrar la hipótesis que sostiene que: existen obras de animación contemporánea desarrolladas por mujeres colombianas que contienen aportes formales, conceptuales, estéticos y poéticos significativos para la investigación en torno a la animación. En primer lugar, se desarrolla un Estado de la cuestión con enfoque crítico, que permite identificar cómo se ha ignorado la participación de mujeres colombianas en los estudios sobre animación. Posteriormente, se caracterizan …

Representando El Buen Queer-Er: Otras Miradas Para Romper La Cisheteronorma En La Animación Infantil-Juvenil, Delicia Aguado Peláez, Patricia Martínez García

GDI. Revista de investigación de Género, Diseño e Innovación

Este artículo explora la ruptura de los estereotipos de género y la representación de la diversidad en series de animación infantil y juvenil creadas por mujeres y disidencias. Partiendo de un análisis de contenido cualitativo desde una perspectiva interseccional, examinamos la construcción de personajes, las dinámicas sexo-afectivas y las relaciones de poder que se encuentran en cuatro producciones: Star vs. Evil Forces (Daron Nefcy), Steven Universe (Rebeca Sugar), She-Ra and the Princesses of Power (ND Stevenson) y The Owl House (Dana Terrace). Todas ellas comparten una serie de características: ofrecen modelos alternativos de feminidad y masculinidad, más allá de …

Colaboración, Discurso Y Técnicas: La Resistencia Simbólica En El Sueño De La Sultana (Isabel Herguera, 2023), Nerea Cuenca-Orellana

GDI. Revista de investigación de Género, Diseño e Innovación

La película El sueño de la sultana (Isabel Herguera, 2023) reinterpreta el cuento utópico homónimo de Rokeya Sakhawat Hossain (1905) realizando una exploración visual que combina animación, archivo y testimonio. El presente trabajo propone un análisis de la obra desde cuatro puntos clave e interrelacionados: la autoría colaborativa y femenina, los discursos visuales, los lenguajes y técnicas, y las formas de resistencia simbólica con el objetivo principal de establecerla como un caso práctico de diseño feminista contemporáneo.

En primer lugar, se examina el proceso de creación de la película como práctica colaborativa transnacional, donde la animación se convierte en un …

Existence, Difference, And The Nigerian Woman: A Feminist Praxis Beyond Patriarchy, Hannah Yemisi Ajewole

Dissertations

This dissertation investigates the postcolonial condition of Nigerian woman through a critical philosophical engagement with feminist theory, focusing particularly on Simone de Beauvoir’s existentialism and Irigaray’s ethics of sexual difference. It interrogates the historical processes through which gender became the basis of hierarchy in the Nigerian society, arguing that colonial and patriarchal override precolonial forms of relationality—marked by dual-sex relationship and age-based hierarchies—into rigid, male-dominated structures of authority and representation. Drawing also on Oyèrónkẹ́ Oyěwùmí’s critique of western gender epistemologies, the work contextualizes the Nigerian woman’s experience within a broader matrix of colonial imposition, cultural displacement, and epistemic violence. This …

Ukrainian Women In The Russo-Ukrainian War: Between Survival And Resistance, Jelena Nikolic Khatatbeh

Dissertations

This dissertation explores how the Russo-Ukrainian war has transformed the lives, identities, and resistance strategies of Ukrainian women. Drawing on in-depth, semi-structured interviews with forty-five Ukrainian women conducted between March 2022 and February 2023, it analyzes how everyday survival, political thought, and civic activism have changed during the war. Using a modified grounded theory approach and a feminist lens, this study examines women’s psychosocial experiences, their evolving political consciousness, and their active roles in humanitarian, cultural, and cyber resistance.Findings reveal that the war simultaneously reinforced and disrupted traditional gender expectations. While Ukrainian women dealt with increased domestic and caregiving burdens …

Analysis Of The Role Of Agricultural Investment Policies And Agricultural Lending In Developing Commodity Production Of Rice Crops, Istibraq Mansour Falih, Omar Hameed Majeed

Journal of Economics and Administrative Sciences

This study investigates the impact of agricultural investment policy—represented by agricultural loans and investment allocations—on rice crop production in Iraq over the period 2003–2023, employing the Autoregressive Distributed Lag (ARDL) model. Using time-series econometric analysis, the study confirms a short-term positive and statistically significant effect of financial support on rice output, while revealing statistically insignificant long-term effects. The presence of a cointegration relationship suggests long-term equilibrium between agricultural policy variables and rice production. However, the absence of causality in the Yamamoto-Toda test implies that structural and institutional inefficiencies may dilute the long-term impact of financial interventions. Practical implications of the …