Digital Commons Network^™

Uncovering The Binding Mechanism Of Mutated Omicron Variants Via Computational Strategies, Sajjad Haider, Nadeem Ahmad, Muhammad Shafiq, Ali Raza Siddiqui, Mohammad Nur-E-Alam, Aftab Ahmed, Zaheer Ul-Haq

Pharmacy Faculty Articles and Research

The COVID-19 pandemic, triggered by the SARS-CoV-2 virus, has resulted in nearly 630 million cases and 6.60 million fatalities globally, as of November 2022. SARS-CoV-2, a species of the Coronaviridae family, has a single-stranded positive-sense RNA genome as well as four main structural proteins (S, E, M, and N) required for viral entrance into target cells. The spike protein (S) influences this entry through interactions with human angiotensin-converting enzyme 2 (hACE2) receptor. The World Health Organization (WHO) recognized numerous variants of concern (VOCs) that involve Alpha, Beta, Gamma, Delta, and Omicron, having multiple mutations within the spike protein, altering infection …

Implementation Of Pictorial Test Instruments To Improve Deaf Students' Sexual Behavior Knowledge Through Direct Learning, Budi Susetyo, Iding Tarsidi, Wiwi Siswaningsih, Andily Aprilia Rahmawati, Nuning Sapta Rahayu, Tati Hernawati

Journal of ICSAR

Adolescents with disability are vulnerable to sexual violence, due to their limitations. This study aims to improve knowledge and understanding of sexual behavior of deaf students by using test instrument in the form of images in direct learning, so it can overcome deviant sexual behavior. The research method used was an experiment with a one group posttest experimental design, the research sample was 22 deaf students of SMPLB and SMALB in the West Java and Bali. The test instrument in the form of choices was equipped with images consisting of four aspects, namely the anatomy and function of reproductive organs, …

Zongertinib (Bi 1810631), An Irreversible Her2 Tki, Spares Egfr Signaling And Improves Therapeutic Response In Preclinical Models And Patients With Her2-Driven Cancers, Birgit Wilding, Lydia Woelflingseder, Anke Baum, Krzysztof Chylinski, Gintautas Vainorius, Neil Gibson, Irene C Waizenegger, Daniel Gerlach, Martin Augsten, Fiona Spreitzer, Yukina Shirai, Masachika Ikegami, Sylvia Tilandyová, Dirk Scharn, Mark A Pearson, Johannes Popow, Anna C Obenauf, Noboru Yamamoto, Shunsuke Kondo, Frans L Opdam, Annemarie Bruining, Shinji Kohsaka, Norbert Kraut, John V Heymach, Flavio Solca, Ralph A Neumüller

Faculty, Staff and Student Publications

Mutations in ERBB2 (encoding HER2) occur in 2% to 4% of non-small cell lung cancer (NSCLC) and confer poor prognosis. ERBB-targeting tyrosine kinase inhibitors, approved for treating other HER2-dependent cancers, are ineffective in HER2-mutant NSCLC due to dose-limiting toxicities or suboptimal potency. We report the discovery of zongertinib (BI 1810631), a covalent HER2 inhibitor. Zongertinib potently and selectively blocks HER2, while sparing EGFR, and inhibits the growth of cells dependent on HER2 oncogenic driver events, including HER2-dependent human cancer cells resistant to trastuzumab deruxtecan. Zongertinib displays potent antitumor activity in HER2-dependent human NSCLC xenograft models and enhances the activities of …

Neutrophil Extracellular Traps Promote Pre-Metastatic Niche Formation In The Omentum By Expanding Innate-Like B Cells That Express Il-10, Wonjae Lee, Song Yi Ko, Hironari Akasaka, Melanie Weigert, Ernst Lengyel, Honami Naora

Faculty, Staff and Student Publications

Disseminated cancer cells in the peritoneal fluid often colonize omental fat-associated lymphoid clusters but the mechanisms are unclear. Here, we identify that innate-like B cells accumulate in the omentum of mice and women with early-stage ovarian cancer concomitantly with the extrusion of chromatin fibers by neutrophils called neutrophil extracellular traps (NETs). Studies using genetically modified NET-deficient mice, pharmacologic inhibition of NETs, and adoptive B cell transfer show that NETs induce expression of the chemoattractant CXCL13 in the pre-metastatic omentum, stimulating recruitment of peritoneal innate-like B cells that in turn promote expansion of regulatory T cells and omental metastasis through producing …

Nerves At Play: The Peripheral Nervous System In Extracranial Malignancies, Paola D Vermeer, Anthony C Restaino, Jeffrey L Barr, Dan Yaniv, Moran Amit

Faculty, Staff and Student Publications

The exponential growth of the cancer neuroscience field has shown that the host's immune, vascular, and nervous systems communicate with and influence each other in the tumor microenvironment, dictating the cancer malignant phenotype. Unraveling the nervous system's contributions toward this phenotype brings us closer to cancer cures. In this review, we summarize the peripheral nervous system's contributions to cancer. We highlight the effects of nerve recruitment and tumor innervation, the neuro-immune axis, glial cell activity, and neural regulation on cancer development and progression. We also discuss harnessing the neural control of peripheral cancers as a potential therapeutic approach in oncology. …

Combined Kras Inhibition And Immune Therapy Generates Durable Complete Responses In An Autochthonous Pdac Model, Yonghong Liu, Jincheng Han, Wen-Hao Hsu, Kyle A Labella, Pingna Deng, Xiaoying Shang, Paulino Tallón De Lara, Li Cai, Shan Jiang, Ronald A Depinho

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) resists conventional chemo/radiation and immunotherapy. In PDAC, oncogenic KRAS (KRAS*) drives glycolysis in cancer cells to consume available glucose and produce abundant lactate, creating profound immune suppression in the tumor microenvironment. Here, we combined KRAS* inhibition with agents targeting the major arms of the immunity cycle: CXCR1/2 inhibitor for myeloid cells, antagonistic anti-LAG3 antibody for T cells, and agonistic anti-41BB antibody for dendritic cells. This combination elicited robust anti-tumor regression in iKPC mice bearing large autochthonous tumors. While untreated mice succumbed within 3 weeks, sustained treatment led to durable complete tumor regression and prolonged survival in …

Agricultural Producers' Motivations And Challenges With Improving Soil Health In Utah, Peggy Petrzelka, Jessica Ulrich-Schad, Matt Yost, Jenae Dean, Tony Richards, Kristy Davis

All Current Publications

The Utah Soil Health Program (USHP) developed a five-year project to increase understanding of how best to implement soil health practices into Utah’s diverse farming systems. Fifteen producers were selected to participate in the USHP On-Farm Soil Health Demonstration Project. During 2022, the trial’s first year, the participating farmers and ranchers were interviewed. We begin by discussing participant demographics and operation characteristics. Next, we turn to challenges the producers faced in implementing soil health practices in the IMW and then turn to motivations for the producers to enroll in the USHP trial. This will help (1) document what is currently …

Grass Weed Control And Rice Response With Tetflupyrolimet-Containing Programs, Mason C. Castner, Jason K. Norsworthy, Thomas R. Butts, Trenton L. Roberts, Nick R. Bateman, Travis R. Faske

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Tetflupyrolimet is the first herbicide with a novel site of action (SOA) labeled PRE and early POST for use in agronomic crops to be labeled in the last three decades. Direct-seeded paddy rice field experiments were conducted near Stuttgart, AR, on a silt loam soil and near Keiser, AR, on a clay soil to evaluate tetflupyrolimet-containing herbicide programs in comparison to commercial standards in conventional, imidazolinone-resistant, and quizalofop-resistant rice systems. Additionally, a furrow-irrigated rice experiment was conducted near Colt, AR, and Keiser to ensure weed control with clomazone and tetflupyrolimet mixtures compared to commercial standards. Twelve commonly planted rice cultivars …

Root Decomposition And Nutrient Dynamics In Multifunctional Forage-Biomass Buffer-Strip Systems, Helen C.S. Amorium, Amanda J. Ashworth, Taylor C. Adams, Mary C. Savin, Philip A. Moore Jr

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Perennial crops are thought as a solution for enhancing food security and providing ecosystem services under a changing climate, including forage-biomass production, reduced erosion and nutrient leaching, and soil C accumulation. However, the drivers of root decomposition and C allocation in perennial multifunctional forage-biomass buffer-strips as affected by fertility management are poorly elucidated. Thus, study objectives were to assess root decomposition and soil CO2 efflux on switchgrass (Panicum virgatum L.), eastern gamagrass (Tripsacum dactyloides L.), silphium (Silphium integrifolium Michx.), and intermediate wheatgrass Kernza (Thinopyrum intermedium [Host] Barkworth & D.R. Dewey) treated with poultry litter (PL) relative to the unfertilized control. …

Lipid Nanoparticles Deliver Dna-Encoded Biologics And Induce Potent Protective Immunity, Dafei Chai, Junhao Wang, Jing Ming Lim, Xiaohui Xie, Xinfang Yu, Dan Zhao, Perry Ayn Mayson Maza, Yifei Wang, Dana Cyril-Remirez, Ken H Young, Yong Li

Faculty, Staff and Students Publications

Lipid nanoparticles (LNPs) for mRNA delivery have advanced significantly, but LNP-mediated DNA delivery still faces clinical challenges. This study compared various LNP formulations for delivering DNA-encoded biologics, assessing their expression efficacy and the protective immunity generated by LNP-encapsulated DNA in different models. The LNP formulation used in Moderna's Spikevax mRNA vaccine (LNP-M) demonstrated a stable nanoparticle structure, high expression efficiency, and low toxicity. Notably, a DNA vaccine encoding the spike protein, delivered via LNP-M, induced stronger antigen-specific antibody and T cell immune responses compared to electroporation. Single-cell RNA sequencing (scRNA-seq) analysis revealed that the LNP-M/pSpike vaccine enhanced CD80 activation signaling …

Identification And Characterization Of A Novel Pavlovskyella (Acari: Argasidae) From Chile, Parasite Of The Culpeo Fox (Lycalopex Culpaeus), Sebastián Muñoz-Leal, Alexander R Kneubehl, Karen Ardiles, Catalina Parragué-Migone, Ignacio Troncoso-Toro, Pablo Oyarzún-Ruiz, Job E Lopez

Faculty, Staff and Students Publications

Soft ticks (Argasidae) of the subgenus Pavlovskyella Pospelova-Shtrom are worldwide distributed parasites of medical importance. However, the systematics of the subgenus are currently under debate because genetic data shows that the group is paraphyletic. Meanwhile, species of Pavlovskyella continue to be discovered. In this study a novel species of the subgenus is described from specimens collected on a fox in central Chile. The larva of this new species differentiates from other Pavlovskyella spp. by having the following combination of characters: subpyriform dorsal plate; 15 pairs of setae, 7 anterolateral, 3 central, and 5 posterolateral, and hypostome with denticles in the …

Skeletal And Dental Tissue Mineralization: The Potential Role Of The Endoplasmic Reticulum/Golgi Complex And The Endolysosomal And Autophagic Transport Systems, Irving M. Shapiro, Makarand V. Risbud, Tengteng Tang, William J. Landis

Department of Orthopaedic Surgery Faculty Papers

This paper presents a review of the potential role of the endoplasmic reticulum/Golgi complex and intracellular vesicles in mediating events leading to or associated with vertebrate tissue mineralization. The possible importance of these organelles in this process is suggested by observations that calcium ions accumulate in the tubules and lacunae of the endoplasmic reticulum and Golgi. Similar levels of calcium ions (approaching millimolar) are present in vesicles derived from endosomes, lysosomes and autophagosomes. The cellular level of phosphate ions in these organelles is also high (millimolar). While the source of these ions for mineral formation has not been identified, there …

Infiltrating Plasma Cells Maintain Glioblastoma Stem Cells Through Igg-Tumor Binding, Jiancheng Gao, Danling Gu, Kailin Yang, Junxia Zhang, Qiankun Lin, Wei Yuan, Xu Zhu, Deobrat Dixit, Ryan C Gimple, Hao You, Qian Zhang, Zhumei Shi, Xiao Fan, Qiulian Wu, Chenfei Lu, Zhangchun Cheng, Daqi Li, Linjie Zhao, Bin Xue, Zhu Zhu, Zhe Zhu, Hui Yang, Ningwei Zhao, Wei Gao, Yingmei Lu, Junfei Shao, Chuandong Cheng, Dapeng Hao, Shuo Yang, Yun Chen, Xiaoming Wang, Chunsheng Kang, Jing Ji, Jianghong Man, Sameer Agnihotri, Qianghu Wang, Fan Lin, Xu Qian, Stephen C Mack, Zhibin Hu, Chaojun Li, Michael D Taylor, Yan Li, Nu Zhang, Jeremy N Rich, Yongping You, Xiuxing Wang

Faculty, Staff and Students Publications

Glioblastoma is a highly aggressive primary brain tumor with glioblastoma stem cells (GSCs) enforcing the intra-tumoral hierarchy. Plasma cells (PCs) are critical effectors of the B-lineage immune system, but their roles in glioblastoma remain largely unexplored. Here, we leverage single-cell RNA and B cell receptor sequencing of tumor-infiltrating B-lineage cells and reveal that PCs are aberrantly enriched in the glioblastoma-infiltrating B-lineage population, experience low level of somatic hypermutation, and are associated with poor prognosis. PCs secrete immunoglobulin G (IgG), which stimulates GSC proliferation via the IgG-FcγRIIA-AKT-mTOR axis. Disruption of IgG-FcγRIIA paracrine communication inhibits GSC proliferation and self-renewal. Glioblastoma-infiltrating PCs are …

Meta-Analysis Of Dedicated Vs Off-Label Transcatheter Devices For Native Aortic Regurgitation, Sahar Samimi, Taha Hatab, Chloe Kharsa, Safi U Khan, Rody G Bou Chaaya, Fatima Qamar, Joe Aoun, Syed Zaid, Nadeen Faza, Marvin D Atkins, Stephen H Little, William A Zoghbi, Michael J Reardon, Neal S Kleiman, Sachin S Goel

Faculty, Staff and Students Publications

Background: Transcatheter aortic valve replacement (TAVR) for high surgical risk patients with severe native aortic regurgitation (AR) presents unique challenges. Dedicated devices such as the JenaValve (JenaValve Technology) and J-Valve (JC Medical Inc) show promising results in addressing these challenges.

Objectives: This study compares the safety and efficacy of dedicated vs off-label devices among high surgical risk patients with pure native AR.

Methods: We systematically searched PubMed, EMBASE, and Cochrane Central Register of Controlled Trials through July 11, 2024, for studies on TAVR among patients with pure severe native AR. The primary endpoint was 30-day all-cause mortality. Secondary endpoints were …

Soluble Herpes Virus Entry Mediator And Type Ii/Iii Interferons Are Upregulated In Primary Biliary Cholangitis, Yooyun Chung, Hio Lam Phoebe Tsou, Michael A Heneghan, Shilpa Chokshi, Antonio Riva

Peninsula Medical School

Bacterial translocation-induced inflammation and immune dysfunction are recognised factors contributing to the pathogenesis of primary biliary cholangitis (PBC). However, the specific involvement of interferons (IFNs) and soluble checkpoints (sol-CRs) in shaping the immune landscape in PBC patients remains unexplored. Furthermore, the influence of ursodeoxycholic acid (UDC) on these immune mediators is unknown. Twenty-eight cytokines and 14 sol-CRs were quantified by Luminex assays in plasma samples from 64 PBC patients and 10 healthy controls (HCs). D-lactate was measured as a marker of bacterial translocation. The PBC subgroups were: 24 UDC responders (UDCRs), 18 UDC non-responders (UDCNRs) and 22 patients with end-stage …

Daily Glucocorticoids Promote Glioblastoma Growth And Circadian Synchrony To The Host, Maria F Gonzalez-Aponte, Anna R Damato, Tatiana Simon, Nigina Aripova, Fabrizio Darby, Myung Sik Jeon, Jingqin Luo, Joshua B Rubin, Erik D Herzog

2020-Current year OA Pubs

Glioblastoma (GBM) is the most common primary malignant brain tumor in adults with a poor prognosis despite aggressive therapy. Here, we hypothesized that daily host signaling regulates tumor growth and synchronizes circadian rhythms in GBM. We find daily glucocorticoids promote or suppress GBM growth through glucocorticoid receptor (GR) signaling depending on time of day and the clock genes, Bmal1 and Cry. Blocking circadian signals, like vasoactive intestinal peptide or glucocorticoids, dramatically slows GBM growth and disease progression. Analysis of human GBM samples from The Cancer Genome Atlas (TCGA) shows that high GR expression significantly increases hazard of mortality. Finally, mouse …

Economic Analysis Of Hay And Corn Production At Morehead State University’S Derrickson Agricultural Complex, Caroline Adkins, Allie N. Dick, Luke Millay, Vijay Subramaniam

Posters-at-the-Capitol

Morehead State University’s Derrickson Agricultural Complex (DAC) produces several agricultural enterprises and provides valuable hands-on experiences for students. Hay and corn are the two dominant crop enterprises which cover 58 percent of the total area. In addition, the DAC produces hay from six different farms using the crop-share leasing method. The primary objectives of this study are to (1) analyze the profitability of hay and corn production at the DAC and leased farms and (2) identify the strengths, and weaknesses of each farm to improve production efficiencies. Two years of data were collected, and the profitability of hay and corn …

Mobility Task Shows Rescue Of Scotopic Vision In A Swine Model For Autosomal Dominant Retinitis Pigmentosa After Treatment With The Meganuclease Rho 1-2, Gita Jaikumar, Jack Feist, Kautuk Kamboj, Aravind Kumar, Francesca Barone, Archana Jalligampala, Wei Wang, Jeff Smith, Dave Morris, Derek Jantz, Victor Bartsevich, Kristi Viles, Maureen Mccall

Posters-at-the-Capitol

Purpose : Retinitis pigmentosa (RP) is the most common form of inherited retinal disease with ~1:5000 people affected worldwide. The substitution of Proline to Histidine (P23H) at position 23 in the rhodopsin protein is the most common form of adRP in North America. We have shown that treating a transgenic P23H human RHO pig model of adRP (TgP23H hRHO) with a gene-editing meganuclease Rho 1-2, recovers rod structure and restores rod function. We ran WT, Rho1-2 treated and untreated TgP23H hRHO (Tg) pigs through an obstacle course to quantify visually guided mobility under light- and dark-adapted conditions using motion analysis …

A Byzantine-Period Monastery And Farmhouse In Nahal Pehar (Giv‘Ot Bar), In The Northern Negev, Nir-Shimshon Paran

'Atiqot

A Byzantine-period monastery and farmhouse were uncovered on the southwestern bank of Naḥal Peḥar, within the modern settlement of Giv‘ot Bar, in the northern Negev. The monastery was well-preserved, attesting to the daily life of the monks in the Negev in the Byzantine period. It was paved with poorly executed mosaic floors, probably laid by the monks themselves. Most of the finds in the monastery were uncovered on the floors, attesting to its orderly abandonment in the late Byzantine or beginning of the Umayyad period (sixth–seventh centuries CE). The monastery seems to have belonged to the widespread ecclesiastical system documented …

Intermediate Bronze Age Fauna From A Suspected Cultic Area At Nahal RefaʼIm, Liora Kolska Horwitz

'Atiqot

A small assemblage of faunal remains was recovered from four archaeological contexts excavated on the northern bank of Naḥal Refaʼim. These include Bronze Age rock-cut pits (Area 100), a structure (Area 300), a burial cave (Area 500) and an Ottoman-period shomera (Area 400). The remains represent almost exclusively domestic taxa—sheep, goat, cattle and donkey—some of which exhibit butchery marks, other modifications or burning. The faunal assemblage from the pits, together with other complementary data, may attest that the pits were used as favissae (cultic storage pits) associated with ritual activities at the site.

Intermediate Bronze Age, Middle Bronze Age Ii And Iron Age Ii Pottery From Nahal Refa’Im, Lilly Gershuny

'Atiqot

The pottery assemblage from the Naḥal Refa’im site originated in three different archaeological contexts: pits, structure(s) and a burial cave. These findings attest that the main period of occupation was during the Intermediate Bronze Age. One pit may have been reused for burial in Middle Bronze Age II, and the burial cave was visited in Iron Age II, possibly by the inhabitants of nearby Khirbat er-Ras.

Intermediate And Middle Bronze Age Settlement Remains, Domestic Worship, Pits And Burial Practices In Nahal RefaʼIm, Jerusalem, Shlomit Weksler-Bdolah, Alexander Onn

'Atiqot

Excavations on the northern bank of the Refa’im Valley uncovered four sites: a shaft tomb and building remains with a standing stone dating to the Intermediate Bronze Age; a concentration of eight pits dating to the Intermediate Bronze Age and the Middle Bronze Age II; and an Ottoman-period agricultural watchtower. The building remains and installations were probably part of the Intermediate Bronze Age settlement along Naḥal Refa’im. The standing stone’s prominent location, and the large concentration of pits and installations near it, may support its identification with domestic worship. The thin fill at the bottom of some of the pits …

Light-Microscopic And Spectroscopic Analyses Of The Skin Materials Used In The Tefillin And Mezuzah Slips From The Judean Desert, Yonatan Adler, Theresa Emmerich Kamper

'Atiqot

Among the Dead Sea Scrolls is an assemblage of ancient tefillin and mezuzah slips—ritual artifacts composed of thin animal skins inscribed with passages from the Pentateuch. This paper presents the results of light-microscopic and spectroscopic analyses conducted on 26 tefillin and 8 mezuzot from the Judean Desert caves. These analyses provide the first opportunity to identify the following characteristics on these artifacts: animal species; skin thickness; skin layers present; tannage; side/s of skin with writing. In time, these characteristics took on ritual significance, especially in later rabbinic halakhah. A significant finding is that the ancient tefillin analyzed here were all …

An Iron Age Iib–C Hebrew Stamp Seal With Apotropaic And Prophylactic Properties From The Southwestern Corner Of The Temple Mount, Jerusalem, Filip Vukosavović, Ronny Reich, Navot Rom, Yuval Baruch

'Atiqot

An Iron Age Hebrew iconic stamp seal was uncovered near the southwestern corner of the Temple Mount in Jerusalem. The seal, made of black stone, depicts a winged genie and a personal name, Yehoʽezer son of Hoshaʽyahu. This is the first depiction of the winged genie iconography in the region found in an archaeological excavation. Influenced by Neo-Assyrian art, the seal was created in Jerusalem by a local artist for Hoshaʽyahu, a member of the Judean elite, during the Assyrian Century. Later on, the seal was inherited by his son, Yehoʽezer. In addition to being used as a stamp, the …

Evidence Of Worship In The Rock-Cut Rooms On The Eastern Slope Of The City Of David, Jerusalem, Eli Shukron, Liora Freud, Helena Roth, Reli Avisar, Efrat Bocher

'Atiqot

This paper presents the finds retrieved from a unique series of rock-cut rooms found on the eastern slope of the City of David. These rock-cut rooms stand out in terms of their architectural features, as well as the artifacts discovered within them, all of which reflect on their function as a cultic complex, likely constructed in the Middle Bronze Age, with its final stage in Iron Age IIB. This complex offers profound evidence for the diversity of cultic practices in the capital of the Kingdom of Judah.

The Emergence Of Worship And Religion In The Neolithic Period: Direct And Indirect Evidence From Moẓa (Motza), Hamoudi Khalaily, Anna Eirikh-Rose, Ianir Milevski

'Atiqot

Evidence of worship and religion in the form of architectural remains is rather scanty before the shift in human behavior, from hunter-gatherers to sedentary communities, and the emergence of permanent settlements. Communal and ritual structures first appeared in the Natufian culture (12,500–9400 BCE) and expanded during the Neolithic period (9400–5300 BCE), alongside the treatment of burials linked to ritual related to ancestor worship. The excavations at Motza’s Final PPNB (7100–6700 BCE) layer exposed many non-residential structures, some presumably used for worship and ritual. Some burials, including the one interpreted here as a shaman burial, included objects of symbolic importance. Cultic …

Enhancing Student Creativity Through Online Learning: A Study Of Spada Indonesia In Civic Education Courses At Universitas Pendidikan Indonesia And Universitas Lambung Mangkurat, Dede Iswandi, Dasim Budimansyah, Kokom Komalasari, Susan Fitriasari

Jurnal Civics: Media Kajian Kewarganegaraan

This study examines the effectiveness of the SPADA Indonesia online learning platform in fostering creativity among students enrolled in Civic Education courses at Universitas Pendidikan Indonesia (UPI) and Universitas Lambung Mangkurat (ULM) Banjarmasin. Given the increasing importance of creativity as a critical skill for future graduates, this research aims to explore how the features and interactive elements of SPADA Indonesia contribute to enhancing student creativity within the context of mandatory Civic Education curricula. A total of 90 students participated in this study, comprising 45 from each university, selected through purposive sampling to ensure a diverse representation of experiences and perspectives. …

Chromosome 4 Duplication Associated With Strabismus Leads To Gene Expression Changes In Ipsc-Derived Cortical Neurons., Mayra Martinez-Sanchez, William C Skarnes, Ashish Jain, Sampath Vemula, Liang Sun, Shira Rockowitz, Mary C Whitman

Faculty Research 2025

BACKGROUND/OBJECTIVES: Strabismus is the most common ocular disorder of childhood. Three rare, recurrent genetic duplications have been associated with both esotropia and exotropia, but the mechanisms by which they contribute to strabismus are unknown. This work aims to investigate the mechanisms of the smallest of the three, a 23 kb duplication on chromosome 4 (hg38|4:25,554,985-25,578,843).

METHODS: Using CRISPR and bridging oligos, we introduced the duplication into the Kolf2.1J iPSC line. We differentiated the parent line and the line with the duplication into cortical neurons using a three-dimensional differentiation protocol, and performed bulk RNASeq on neural progenitors (day 14) and differentiated …

Beauty And Gore: Unsettling Audiences In The 2018 Production Of Ang Paglilitis Ni Mang Serapio, Sophia Martha B. Marco, Svetlana Antropova

Perspectives in the Arts and Humanities Asia

No abstract provided.

Beauty And Gore: Unsettling Audiences In The 2018 Production Of Ang Paglilitis Ni Mang Serapio, Sophia Martha B. Marco, Svetlana Antropova

Perspectives in the Arts and Humanities Asia

No abstract provided.