Digital Commons Network^™

Redefining Cell Culture Using A 3d Flipwell Co-Culture System: A Mimetic For Gut Architecture And Dynamics In Vitro, Saori Furuta

Faculty Scholarship

Gut mucosae are composed of stratified layers of microbes, a selectively permeable mucus, an epithelial lining, and connective tissue homing immune cells. Studying cellular and chemical interactions between the gut mucosal components has been limited without a good model system. We have engineered a three-dimensional (3D) multi-cellular co-culture system we coined “3D Flipwell system” using cell culture inserts stacked against each other. This system allows an assessment of the impact of a gut mucosal environmental change on interactions between gut bacteria, epithelia, and immune cells. As such, this system can be utilized in examining the effects of exogenous stimuli, such …

Detection Of Allergen Mango Components By Using Droplet Digital Pcr Technology, Li Enjing, Wang Dan, Li Long, Li Hejing, Yang Honglian

Food and Machinery

［［Objective ］］ A droplet digital PCR method for detect allergen mango components was established in this study.[Methods ] Firstly,the chloroplast trnH-psbA gene was selected as the target gene for detect allergen mango components.Secondly,specific primers and probe were designed.Finally,the reaction conditions of droplet digital PCR was optimized to verify the sensitivity and stability of the method.[Results ] This method had been applied to detect allergen mango components in 10 batches of food samples.The results of establishment of droplet digital PCR method was showed good specificity and not cross -react with 10 kinds of other fruits except mango.Under the optimal ddPCR reaction …

Online Simultaneous Determination Of Lactoferrin And Immunoglobulin G In Dairy Products By Two-Dimensional Liquid Chromatography, Chen Qin, Zhou Langjun, Chen Zijing, Liu Mengni, Lin Senyu

Food and Machinery

［［Objective ］］ A method of two -dimensional liquid chromatograohy was established to simultaneously determine the content of lactoferrin and immunoglobulin G in dairy products.[Methods ] The pretreated samples were directly injected into the constructed system.Target protein and other effects were separated on a Pharmacia HI -Trap Protein G as the first -dimensional column using a mobile phase consisting of sodium phosphate buffer and glycocoll buffer by gradient elution lactoferrin and immunoglobulin G were separated on a BioCore SEC -300 (4.6 mm ×250 mm,5 μm) as the second -dimensional column using a mobile phase consisting of water and sodium phosphate buffer …

The Effect Of Ultrafine Tianma Powder On The Behavior Of Motion Sickness Model Mice, Zhu Hongri, Li Jianxiang, Wang Zhaojun, Chen Yonghong

Food and Machinery

［［Objective ］］ To solve the problem of adverse side effects of anti -vertigo drugs and to develop functional foods with the same source of medicine and food as anti -motion sickness.［［Methods ］］ After 7 days of adaptive feeding,50 mice were divided into normal group,model group,positive drug group,Gastrodia H group and Gastrodia L group,and the mouse motion sickness model was constructed by rotational stimulation method,and the ultrafine powder of Gastrodia (gastrodin content 1.05%) was administered by gavage,and the treatment effect of Gastrodia on motion sickness model mice was evaluated by behavioral indicators such as motion sickness reaction index,balance beam test and …

Determination Of Chlorothalonil And Its Metabolite 4-Hydroxychlorothalonil Residues In Animal-Derived Food By Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry, Liu Xiaofang, Li Zheng, He Hao, Jiang Junjie, Chen Yuanyuan

Food and Machinery

［［Objective ］］ To establish a method for simultaneous determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal -derived food by ultra -high performance liquid chromatography -tandem mass spectrometry.［［Methods ］］ The samples were extracted by oscillating with acetonitrile solution of 1% acetic acid,defatted with n -hexane,purified by matrix dispersible solid phase extractant (PSA,anhydrous magnesium sulfate ),separated by Waters BEH C18 column under a gradient elution program using 0.1% formic acid aqueous solution and acetonitrile as the mobile phases.The anion multiple reaction monitoring model (MRM ) of atmospheric pressure chemical ionization source (APCI ) was used for the determination,and the matrix …

Effect Of Chicory Polysaccharide On The Texture And Storage Quality Of Round Scad Fish Ball, Xue Shan, Lin Jingjuan

Food and Machinery

［［Objective ］］ The processing technology of round scad (Decapterus maruadsi) fish balls with chicory polysaccharide was optimized and its storage characteristics were analyzed.[Methods ] The effects of chicory polysaccharide (CP) supplemental level,corn starch supplemental level,salt supplemental level,water supplemental level,complex phosphate supplemental level and gel temperature on the texture and comprehensive score of scad fish balls were investigated.The processing technology of chicory polysaccharide scad fish balls was optimized based on the comprehensive score.The processing technology of chicory polysaccharide scad fish balls was optimized based on the comprehensive score.The microstructure of the fish balls was observed by optical microscope and scanning electron …

Research Progress On Digestion , Absorption And Health Efficacy Of Astaxanthin Ester, Du Yufeng, Che Hongxia, Li Xiaoran, Zhou Miaomiao, Hou Peng

Food and Machinery

Astaxanthin is primarily found in both free and esterified forms in nature,With astaxanthin esters presenting various structural variations.These forms differ significantly in their digestive,absorption,and health -related functions compared to free astaxanthin.This review provides a comprehensive overview of the latest research regarding the sources,digestion and absorption characteristics,and health benefits of astaxanthin esters,based on an analysis of domestic and international studies.It examines how the presence of fatty acids affects the digestion and absorption of astaxanthin ester and discusses their functional activity and potential mechanisms.Additionally,the review highlights existing challenges in the development and utilization of astaxanthin esters.

Determination Of Tetraniliprole Residues In Animal Derived Foods By Multi-Plug Filtration Cleanup Combined With Hplc-Ms/Ms, Yuan Liejiang, Wang Xiu, Li Zheng, Zhu Li, Deng Hang

Food and Machinery

［［Objective ］］ This study aimed to develop a rapid and efficient method for quantifying tetraniliprole residues in animal -derived foods by combining multi -plug filtration cleanup (m-PFC ) with high -performance liquid chromatography -mass spectrometry (HPLC -MS).［［Methods ］］ Samples were extracted with acetonitrile,followed by salting out using sodium chloride and anhydrous magnesium sulfate.The extracts were purified via m -PFC and analyzed using HPLC -MS with external standard calibration.［［Results］］ The method demonstrated a strong linear correlation between tetraniliprole concentration and response values over a range of 0.001 to 0.150 mg/L,with a coefficient of determination (R2) exceeding 0.999.The limit of quantification (LOQ …

Research Progress On Different Pickling Techniques And Their Equipment Applications, Dong Junyi, Yao Huanli, Chen Shuai, Lu Jing, Xu Zeyu

Food and Machinery

The traditional pickling method for meat has a slow pickling speed and uneven pickling solution,which can easily lead to unstable taste and color of the meat.With the deepening of research in recent years,the improvement mechanism of new technologies such as ultra -high voltage and pulsed electric field on the quality of meat pickling has gradually been elucidated and applied in meat pickling.The article reviews the research progress of different pickling techniques and their equipment applications,including ultrasonic pickling technology,vacuum rolling pickling technology,variable pressure rolling pickling technology,ultra -high pressure pickling technology,and pulse electric field technology.The advantages and disadvantages of these techniques and …

Nondestructive Detection Of Chilled Mutton Ph Value Using Hyperspectral Imaging Technique, Li Mengyuan, Zhang Wenguang, Jiang Xinhua, Xu Ziyang, Shi Caixia

Food and Machinery

［Objective］ To detect the pH value of chilled mutton quickly and non -destructively.［Methods］ Visible/near -infrared hyperspectral (400~1 000 nm) imaging technology was used to collect scattering images on the surface of chilled mutton,and the reflectance spectral curve of the region of interest of the sample was extracted to obtain the original spectral data.Four single methods of savitzky -golay (SG),multiplicative scatter correction (MSC ),standard normal variant transformation (SNV ) and first -order derivative (FD) and three combination methods of MSC -SG,SNV -SG and FD -SG were used to preprocess the original spectral data.Linear regression model:partial least squares regression (PLSR ) and …

Impact Of Voluntary Exercise On Systemic Inflammation In A 2? Hit Model Of Heart Failure With Preserved Ejection Fraction, Heather Quiriarte, Eunhan Cho, Youyoung Kim, Jake E. Doiron, Gregory Davis, James Stampley, Robert C. Noland, Zhen Li, Sanjiv J. Shah, Brian A. Irving, Guillaume Spielmann, David J. Lefer, Timothy D. Allerton

Faculty Publications

No abstract provided.

Minutes, Curriculum Committee Meeting, Tuesday, February 18, 2025, Curriculum Committee

Curriculum Committee Minutes

No abstract provided.

Automated And Assisted Identification Of Stroke Using Feature-Based Brain Imaging, Karl A. Kasischke, William Scott Burgin

USF Patents

Provided herein are systems and methods for automated identification of volumes of interest in volumetric brain images using artificial intelligence (AI) enhanced imaging to diagnose and treat acute stroke. The methods can include receiving image data of a brain having header data and voxel values that represent an interruption in blood supply of the brain when imaged, extracting the header data from the image data, populating an array of cells with the voxel values, applying a segmenting analysis to the array to generate a segmented array, applying a morphological neighborhood analysis to the segmented array to generate a features relationship …

Synthetic Osteobiologics In Spine Surgery: A Review, Aydin Si̇nan Apaydin, Eli Johnson, Prince Antwi, Brett Rocos, Michael M. Haglund, Christopher Shaffrey, Peter Passias, Muhammad Adb-El-Barr, Khoi Than

Turkish Journal of Medical Sciences

Background/aimOsteobiologics are increasingly used in orthopedics and spine surgery to facilitate bone healing and prevent non-union. Synthetic osteobiologics are artificial materials crafted in laboratories that aim to replicate the natural composition and functionality of bone. Notable examples, such as calcium phosphate and calcium sulfate, are engineered to mirror the mineral aspect of bone. They mimic human bone functionality, exhibiting osteoconductive, osteoinductive, and osteogenic properties. These characteristics promote cell attachment, migration, recruitment, and differentiation. Consequently, synthetic osteobiologics (osteoconductive grafts) have been introduced in bone fracture repair. The main strength of synthetic osteobiologics in spine surgery lies in improving fusion rates and …

Empagliflozin Effects On Cyclophosphamide-Induced Neurotoxicity In Rats Without Diabetes, Şeri̇fe Ezgi̇ Doğan, Şeri̇fe Mehli̇ka Kuşkonmaz, Pinar Celepli̇, Mehmet Şenes, Sema Hücümenoğlu, Cavi̇t Çulha

Turkish Journal of Medical Sciences

Background/aim: The objective of this study is to evaluate the effect of empagliflozin on cyclophosphamide-induced neurotoxicity in rats.Materials and methods: A total of 32 male Wistar rats were separated into 4 groups as 8 rats each: control group, cyclophosphamide group, empagliflozin group and the cyclophosphamide plus empagliflozin group. At the end of the experiment rats' brains were removed for biochemical analysis of oxidative stress parameters (malondialdehyde, total sulfhydryl, total oxidant status and total antioxidant capacity) and histopathological analysis.Results: Total sulfhydryl levels were increased in group empagliflozin and group empagliflozin and cyclophosphamide compared to the cyclophosphamide group but did not improve …

Relationship Between The Density Of Degenerated Neurons In The Carotid Body And Changes In Blood Ph, Hakan Kina, Aydin Si̇nan Apaydin

Turkish Journal of Medical Sciences

Background/aimCarotid artery is the majority artery for brain feeding. Carotid Bodies (CBs) are located in carotid bifurcation and regulate body fluid pH. We aimed to evaluate the changes in CB-related pH levels as a consequence of degeneration induced by the ligation of both Common Carotid Arteries (BCCAL).Materials and methodsWe included 23 adult male New Zealand rabbits. A control group (n= 5); a sham group (0.5 mL saline injected n=5) and the main study group (subjected to the BCCAL procedure, n = 13). We monitored and recorded pH levels, and cardiac rhythms before, during, and after the experiments at two-day intervals. …

Impact Of Ivermectin On Nerve Regeneration Following Sciatic Injury In Mice: The Consequences Of Dietary High Fructose, Ezgi̇ Deni̇z Arikan, Barişcan Çi̇men, Ayşe Ece Gezen Yilmaz, Eli̇f Akaydin, Berkay Alpay, Deni̇z Eki̇n Erbaş, Erblina Nikshiqi, Sevda Müftüoğlu, Yildirim Sara

Turkish Journal of Medical Sciences

Background/aimPeripheral nerve injuries (PNI) are debilitating disorders affecting predominantly the younger generation, often leading to significant disabilities. Current treatment strategies are inadequate in addressing the complex nature of these injuries. Peripheral nerve healing and functional recovery are crucial components of both pathophysiology and therapeutic approach. High fructose corn syrup (HFCS) is a frequently used sweetener in several beverages and foods. And it is associated with several metabolic disturbances including insulin resistance and may impair nerve healing. This study investigates the therapeutic role of ivermectin on nerve regeneration following sciatic nerve injury and evaluates motor and sensorial functions together with histopathological …

Are Cinnamon Derivatives Effective And Safe For Diabetes?, Methi̇ye Mancak, Ufuk Koca Çalişkan

Turkish Journal of Medical Sciences

Background/aimCinnamon spice is obtained by drying the tree bark of the Cinnamomum Schaeff genus. Cinnamomum genus belongs to the Lauraceae family and there are approximately 250 different species around the world. The most common species in the market are Cinnamomum verum J. S. Presl, C. cassia (L.) J. Presl, C. burmannii (Nees & T. Nees) Blume and C. loureiroi Nees. Cinnamon and its by-products are used for many years for their antidiabetic effect. In this research, the major chemical content and in vitro antidiabetic activities of different commercially available cinnamon samples were evaluated to conclude whether they are safe to …

Association Of Certain Biochemical Parameters Related To Bone Cycle With Genotype In Mps Iii-B Patients, Seda Gökkurt, İrem Peker Eyüboğlu, Banu Nur Güzel, Ercan Mihçi, Ayşe Özer, Mustafa Akki̇pri̇k

Turkish Journal of Medical Sciences

Background/aim: The aim of this study is to investigate the genotype-phenotype correlation in Sanfilippo type B patients in terms of bone formation/resorption parameters, and to determine the release/inhibition of biomarkers accompanying osteoporosis.Materials and Methods: Plasma levels of Osteoprotegerin, Matrix metalloproteinases (MMP2, MMP9), TIMP-1, TIMP-2, BALP, Cathepsin K, and TRACP-5b were examined by ELISA method in MPS IIIB patients. At the same time, mutations in the NAGLU gene causing this disease were detected by whole exome sequencing, and their correlation with biochemical parameters was investigated.Results: Based on the enzyme analysis results, MMP-9, TIMP1, TIMP-2, and MMP-2 were significantly high, while Cathepsin-K …

Impact Of Voluntary Exercise On Systemic Inflammation In A 2? Hit Model Of Heart Failure With Preserved Ejection Fraction., Heather Quiriarte, Eunhan Cho, Youyoung Kim, Jake E. Doiron, Gregory Davis, James Stampley, Robert C. Noland, Zhen Li, Sanjiv J. Shah, Brian A. Irving, Guillaume Spielmann, David J. Lefer, Timothy D. Allerton

School of Medicine Faculty Publications

No abstract provided.

Interview With Helen Carlson And Paul Nicholson, Bailie Jansons

Gordon and Dorothy Carlson Commemoration

No abstract provided.

Crème De La Conference: 8th World Congress Of Pediatric Cardiology And Cardiac Surgery-Wcpccs 2023, Carissa M. Baker-Smith, Liesl Zühlke, Jennifer Co-Vu, Shubhika Srivastava, Renelle George, Shelby Kutty, Ali N. Zaidi, David J. Goldberg, Erica D. Sood, Kathryn M. Dodds, David S. Cooper, Mitchel I. Cohen, Gil Wernovsky, Jeffrey P. Jacobs

Department of Medicine Faculty Papers

No abstract provided.

Allostatic Load And Incident Dementia Among Older Adults In The United States, Mohammed Usman Kabeto

Walden Dissertations and Doctoral Studies

Allostatic load (AL), the cumulative physiological burden of chronic stress, is a significant health problem, and it has been associated with adverse health outcomes, including cognitive functioning. There is a paucity of literature examining associations between AL and incident dementia in the United States. This quantitative study used data from the United States Health and Retirement Study cohort (2016-2020) to examine the association between AL quartile and incident dementia among participants who were aged 56 and older at baseline. Dementia status was determined measuring immediate and delayed recall, serial seven subtraction, and backward count, as well as self-report conditions for …

Paradigm Shift In Obesity Treatment: An Extensive Review Of Current Pipeline Agents, Ecesu Çeti̇n, Brian Pedersen, Mehmet Furkan Burak

Turkish Journal of Medical Sciences

Obesity is a multifaceted disease that poses a significant public health challenge. Recent discoveries in understanding the biological pathways that regulate satiety and metabolism have led to a shift in the treatment paradigm for obesity. Thus, the gap between pharmacological and surgical interventions has diminished. The latest approved anti-obesity medications are achieving weight loss comparable to surgery. These GLP-1 analog-based therapies not only cause substantial weight loss but also improve obesity-associated comorbidities. However, there are still unmet needs in obesity care, and treatment options with alternative pathways are necessary. Whether achieved through lifestyle changes or medication, weight loss often leads …

The Abdominal Compliance Index And Postoperative Pain After Laparoscopic Gynecologic Surgery: A Preliminary Observational Cohort Study, Ebru Akgün Şarer, Mustafa Tahtaci, Umut Cahi̇t Ersoy, Mehtap Honca

Turkish Journal of Medical Sciences

Background/aimLaparoscopic surgery in gynecology has advantages of reduced scarring, less postoperative pain, faster recovery and lower risk of complications. Despite these adavantages, shoulder pain still remains a major concern affecting upto 90% of patients undergoing gynecologic laparoscopic surgery. The use of Abdominal compliance index (ACI) may offer the benefits of gaining work space for safer surgery and may prevent the drawbacks of applying higher insuflation pressures.We investigated the relation of shoulder pain and abdominal pain and analgesic use with the recently advised ACI which is a surrogate index of abdominal expansion in patients undergoing gynecologic laparoscopic surgery.Materials and methodsThirty one …

Ascorbic Acid Exhibits More Of A Protective Effect Than Estradiol Against Nephrotoxicity Induced By Malathion In Rats: A Histopathological And Molecular Docking Study, Mohammad Alhilal, Mahmoud Salem, Ahmed Albakoush, Suzan Alhilal, Basant Farag, Sobhi M. Gomha

Turkish Journal of Medical Sciences

Background/Aim: Despite the known harmful effects associated with malathion toxicity in various organs, it continues to be widely used for plant protection and insect control. This study is the first to compare the protective effects of estradiol and ascorbic acid against malathion-induced nephrotoxicity through histopathological assessment and molecular docking analyses.

Materials and Methods: This study was conducted using 20 female albino rats that were distributed into sham, malathion, malathion + estradiol, and malathion + ascorbic acid groups. Nephrotoxicity was induced by daily treatment with malathion, and the effects of estradiol and ascorbic acid on nephrotoxicity were evaluated. After 4 weeks …

Detection Of Ceftiofur Sodium In Milk By A Molecular-Imprinted Electrochemical Sensor, Liu Yahui, Gao Hongli, Li Zhaozhou, Chen Xiujin, Wang Yao, Niu Huawei

Food and Machinery

［Objective］ A molecular -imprinted electrochemical sensor was developed to detect ceftiofur sodium in milk.［Methods］ Firstly,a molecular -imprinted film was prepared on the surface of a gold electrode by electropolymerization with ceftiofur sodium as a template molecule and acrylamide as a functional monomer in phosphate buffer solution.The electrochemical performance of the sensor was characterized by differential pulse voltammetry and cyclic voltammetry,and the construction parameters and detection conditions were optimized for the sensor.Under the optimal conditions,the sensor was used to quantitatively determine the content of ceftiofur sodium in milk.［Results］］ The sensor constructed under the optimal conditions exhibited a good linear detection range …

Detection Of Egg Appreance Based On Fasternet And Yolov 5 Model, Wei Jingxin, Chen Zhongju, Xu Haoran

Food and Machinery

［Objective ］ Efficiently identify eggs with defects on their appearance in the automatic production process.［Methods ］ Designed a detection model based on fusing Fasternet module and YOLOv 5s.The model used the Kmeans ++ algorithm to re -cluster the dataset and optimizeed the bounding box.The Bottleneck module in the C 3 structure was replaced by the Fasternet Block module to reduce the parameters and improve the percision in the process of detection.The Soft -NMS,a non -maximum suppression was utilized to improve the detection of eggs with similar features.The CBAM attention mechanism was introduced to enhance the function of extracting important features.［Results］ …

Biguanides Antithetically Regulate Tumor Properties By The Dose-Dependent Mitochondrial Reprogramming-Driven C-Src Pathway, Jun Hyoung Park, Kwang Hwa Jung, Dongya Jia, Sukjin Yang, Kuldeep S Attri, Songyeon Ahn, Divya Murthy, Tagari Samanta, Debasmita Dutta, Meron Ghidey, Somik Chatterjee, Seung Yeop Han, Diego A Pedroza, Abha Tiwari, Joyce V Lee, Caitlin Davis, Shuting Li, Vasanta Putluri, Chad J Creighton, Nagireddy Putluri, Lacey E Dobrolecki, Michael T Lewis, Jeffrey M Rosen, José N Onuchic, Andrei Goga, Benny Abraham Kaipparettu

Faculty, Staff and Students Publications

The biguanide metformin attenuates mitochondrial oxidation and is proposed as an anti-cancer therapy. However, recent clinical studies suggest increased proliferation and fatty acid β-oxidation (FAO) in a subgroup of patients with breast cancer (BC) after metformin therapy. Considering that FAO can activate Src kinase in aggressive triple-negative BC (TNBC), we postulate that low-dose biguanide-driven AMPK-ACC-FAO signaling may activate the Src pathway in TNBC. The low bioavailability of metformin in TNBC xenografts mimics metformin's in vitro low-dose effect. Pharmacological or genetic inhibition of FAO significantly enhances the anti-tumor properties of biguanides. Lower doses of biguanides induce and higher doses suppress Src …

Erfa Faculty Research And Creative Activity Award Rfp, San Jose State University, Emeritus And Retired Faculty Association

ERFA Documents and Policies

No abstract provided.