Digital Commons Network^™

Membrane Lipids Augment Cell Envelope Stress Signaling Via The Madrs System To Defend Against Antimicrobial Peptides And Antibiotics In Enterococcus Faecalis, William R Miller, April Nguyen, Kavindra V Singh, Samie Rizvi, Ayesha Khan, Sam G Erickson, Stephanie L Egge, Melissa Cruz, An Q Dinh, Lorena Diaz, Philip C Thornton, Rutan Zhang, Libin Xu, Danielle A Garsin, Yousif Shamoo, Cesar A Arias

Faculty, Staff and Student Publications

Enterococci have evolved resistance mechanisms to protect their cell envelopes against bacteriocins and host cationic antimicrobial peptides (CAMPs) produced in the gastrointestinal environment. Activation of the membrane stress response has also been tied to resistance to the lipopeptide antibiotic daptomycin. However, the actual effectors mediating resistance have not been elucidated. Here, we show that the MadRS (formerly YxdJK) membrane antimicrobial peptide defense system controls a network of genes, including a previously uncharacterized 3-gene operon (madEFG) that protects the Enterococcus faecalis cell envelope from antimicrobial peptides. Constitutive activation of the system confers protection against CAMPs and daptomycin in the absence of …

Zika Virus Ns1 Drives Tunneling Nanotube Formation For Mitochondrial Transfer And Stealth Transmission In Trophoblasts, Rafael T Michita, Long B Tran, Steven J Bark, Deepak Kumar, Shay A Toner, Joyce Jose, Indira U Mysorekar, Anoop Narayanan

Faculty, Staff and Students Publications

Zika virus (ZIKV) is unique among orthoflaviviruses in its vertical transmission capacity in humans, yet the underlying mechanisms remain incompletely understood. Here, we show that ZIKV induces tunneling nanotubes (TNTs) in placental trophoblasts which facilitate transfer of viral particles, proteins, mitochondria, and RNA to neighboring uninfected cells. TNT formation is driven exclusively via ZIKV non-structural protein 1 (NS1). Specifically, the N-terminal 1-50 amino acids of membrane-bound ZIKV NS1 are necessary for triggering TNT formation in host cells. Trophoblasts infected with TNT-deficient ZIKV

Effects Of Acute Pro-Inflammatory Stimulation And 25-Hydroxycholesterol On Hippocampal Plasticity And Learning Involve Nlrp3 Inflammasome And Cellular Stress Responses, Yukitoshi Izumi, Kazuko A O'Dell, Steven Mennerick, Charles F Zorumski

2020-Current year OA Pubs

Neuroinflammation is an increasingly important target for therapeutics in neuropsychiatry and contributes to cognitive dysfunction, disability and death across a range of illnesses. We previously found that acute effects of pro-inflammatory stimulation with lipopolysaccharide (LPS) on hippocampal long-term potentiation (LTP), a form of synaptic plasticity involved in learning and memory, requires synthesis of the oxysterol, 25-hydroxycholesterol (25HC) and exogenous 25HC mimics effects of LPS. However, downstream mechanisms engaged by LPS and 25HC remain uncertain. Here we use rat hippocampal slices and in vivo behavioral studies to provide evidence that acute modulation of synaptic plasticity by both LPS and 25HC requires …

The Important Role Of Pollinators In Sustainability Education, Bronwen Cowie, Paula Mildenhall

Research outputs 2022 to 2026

In this chapter we illustrate some of the ways the education sector can play a key role, not just in educating and activating students as advocates for and agents of sustainability but also in motivating and mobilising communities towards a sustainability agenda. Initially in this chapter it was important to look back and identify how important bees have been through the ages, noting that humans identified very early on that bees could enhance their lives. We then outline some of the contributions a focus on bees can make to sustainability education. One important contribution includes fostering a balanced perspective on …

An Attention-Based Model For Recognition Of Facial Expressions Using Cnn-Bilstm, Sushil Kumar Singh, Manish Kumar, Ikram Majeed Khan, A. Jayanthiladevi, Chirag Agarwal

Polytechnic Journal

In recent studies, computer vision and human-computer interaction have focused heavily on facial expression recognition (FER). Traditional deep learning algorithms have faced significant challenges when processing images with occlusion, uneven lighting, and positional inconsistencies and addressing dataset imbalances. These issues often result in low accuracy, unreliable recognition rates, and slow convergence. To address the challenges posed by non-frontal visual features, this study proposes a hybrid model that fusion of Convolutional Neural Networks (CNN) and Bidirectional Long Short-Term Memory (BiLSTM), augmented with a point-multiplication attention model and Linear Discriminant Analysis (LDA). Data preparation incorporates median filtering and global contrast normalization to …

Antibiotic-Induced Loss Of Gut Microbiome Metabolic Output Correlates With Clinical Responses To Car T-Cell Therapy, Rishika Prasad, Abdur Rehman, Lubna Rehman, Faezeh Darbaniyan, Viktoria Blumenberg, Maria-Luisa Schubert, Uria Mor, Eli Zamir, Sabine Schmidt, Tomo Hayase, Chia-Chi Chang, Lauren Mcdaniel, Ivonne Flores, Paolo Strati, Ranjit Nair, Dai Chihara, Luis E Fayad, Sairah Ahmed, Swaminathan P Iyer, Michael Wang, Preetesh Jain, Loretta J Nastoupil, Jason Westin, Reetakshi Arora, Joel Turner, Fareed Khawaja, Ranran Wu, Jennifer B Dennison, Meghan Menges, Melanie Hidalgo-Vargas, Kayla Reid, Marco L Davila, Peter Dreger, Felix Korell, Anita Schmitt, Mark R Tanner, Richard E Champlin, Christopher R Flowers, Elizabeth J Shpall, Samir Hanash, Sattva S Neelapu, Michael Schmitt, Marion Subklewe, Johannes Francois-Fahrmann, C K Stein-Thoeringer, Eran Elinav, Michael D Jain, Eiko Hayase, Robert R Jenq, Neeraj Y Saini

Faculty, Staff and Student Publications

Antibiotic (ABX)–induced microbiome dysbiosis is widespread in oncology, adversely affecting outcomes and side effects of various cancer treatments, including immune checkpoint inhibitors and chimeric antigen receptor T-cell (CAR-T) therapies. In this study, we observed that prior exposure to broad-spectrum ABXs with extended anaerobic coverage such as piperacillin-tazobactam and meropenem was associated with worse anti-CD19 CAR-T therapy survival outcomes in patients with large B-cell lymphoma (N = 422) than other ABX classes. In a discovery subset of these patients (n = 67), we found that the use of these ABXs was in turn associated with substantial dysbiosis of gut microbiome function, …

Aif3 Splicing Variant Elicits Mitochondrial Malfunction Via The Concurrent Dysregulation Of Electron Transport Chain And Glutathione-Redox Homeostasis, Mi Zhou, Shuiqiao Liu, Yanan Wang, Bo Zhang, Ming Zhu, Jennifer E Wang, Veena Rajaram, Yisheng Fang, Weibo Luo, Yingfei Wang

Faculty, Staff and Student Publications

Genetic mutations in apoptosis-inducing factor (AIF) have a strong association with mitochondrial disorders; however, little is known about the aberrant splicing variants in affected patients and how these variants contribute to mitochondrial dysfunction and brain development defects. We identified pathologic AIF3/AIF3-like splicing variants in postmortem brain tissues of pediatric individuals with mitochondrial disorders. Mutations in AIFM1 exon-2/3 increase splicing risks. AIF3-splicing disrupts mitochondrial complexes, membrane potential, and respiration, causing brain development defects. Mechanistically, AIF is a mammalian NAD(P)H dehydrogenase and possesses glutathione reductase activity controlling respiratory chain functions and glutathione regeneration. Conversely, AIF3, lacking these activities, disassembles mitochondrial complexes, increases …

Future Climate-Driven Habitat Loss And Range Shift Of The Critically Endangered Whitefin Swellshark (Cephaloscyllium Albipinnum), Kerry Brown, Robert Puschendorf

School of Biological and Marine Sciences

Climate change is driving many species to shift their geographical ranges poleward to maintain their environmental niche. However, for endemic species with restricted ranges, like the Critically Endangered whitefin swellshark (Cephaloscyllium albipinnum), endemic to southeastern Australia, such dispersal may be limited. Nevertheless, there is a poor understanding of how C. albipinnum might spatially adjust its distribution in response to climate change or whether suitable refugia exist for this species in the future. Therefore, to address this gap, this study utilised maximum entropy (MaxEnt) modelling to determine the potential distribution of suitable habitat for C. albipinnum under present-day (2010–2020) climate conditions …

Shalom: A Judeo-Christian Response To Greed, Kwok Tung Cheung

Journal of Religion and Business Ethics

In my recent conceptual analysis of greed, I argue that there are three different conceptual aspects, namely, a consuming desire to acquire more and more (the psychological), an inordinate love for material possession and power (the metaphysical), and an action of acquiring more than one’s share by taking what belongs to one’s fellow citizens, essentially with a calculating indifference toward others (the socio-historical). The aim of that analysis, as a foundational work, is to make sense of the vice or the moral mistake of greed in the contemporary world, especially by reviving the socio-historical aspect which is about justice. Building …

The Future Of Higher Education: Reconciling Freedom Of Expression With Trauma-Informed Practices And Relationship-Building, Michael J. Kaufman, Jennifer R. Stukenberg

Widener Commonwealth Law Review

Our nation’s universities face significant challenges, including providing greater access and affordability, managing a business model heavily dependent on tuition revenue in an era of shifting demographics and declining enrollment, integrating technology and artificial intelligence, and mitigating serious enterprise risks.

The Dirty Work Of Faith-Washing In A Faith-Friendly Business, Cathy Driscoll

Journal of Religion and Business Ethics

Drawing on faith/spirituality at work and organizational legitimacy literatures, a case study of a large global meat company is used to deconstruct the concept of faith-friendly business and explore how faith-washing can provide a business with legitimacy and potentially distract stakeholders from irresponsible activities. In particular, I focus on the faith-friendly communications and initiatives in relation to treatment of non-salaried employees as a primary stakeholder of the company. I highlight a disconnect from religious social thought on meaningful and dignified work and many of the concepts found in the modern faith/spirituality at work literature, as well as societal expectations on …

Ischemia And Anatomy In The New 2024 European Guidelines For Chronic Coronary Syndromes: Update And Comparison Of Recommendations, Christian Adrián Caroli

Journal of the Saudi Heart Association

The new 2024 European guideline on chronic coronary syndromes is a pivotal document for clinical practice, updating the evidence and indications after five years, incorporating insights from the paradigm-shifting ISCHEMIA trial. This article explores the evolving role of functional and anatomical testing in assessing coronary artery disease, highlighting the introduction of a new risk probability score based on clinical and risk factors. Additionally, it provides a detailed comparison between these European recommendations and those from the most influential American guidelines, emphasizing key differences in the approach to risk stratification and diagnostic strategies. This comprehensive analysis provides valuable insights for optimizing …

A Long-Term Study Of Algal Blooms In Alexandria's Ecosystems: A Review, Samia K. Mikhail, Wagdy Labib

Blue Economy

Coastal ecosystems in Alexandria are facing significant stress, primarily due to the intrusion of discharged wastewaters of different origins. Over the past three decades, there has been a noticeable increase in the occurrence of harmful algal blooms (HABs) across Alexandria marine, brackish, and freshwater environments. Unfortunately, the situation is deteriorating, and HABs have become chronic issues characterized by frequent occurrences of various causative species. This phenomenon has spread widely affecting previously uninfected coastal areas with high frequency and diverse patterns of occurrence. This article provides an overview of the state of visible HABs associated with incidents of fish and/or invertebrate …

Exploring The Potential Of Oncolytic Viruses As A Novel Therapeutic Approach For Breast Cancer Treatment, Renad Alsayyad, Claudia M. Tellez Freitas

Annual Research Symposium

No abstract provided.

Shock Effects Including Maskelynite Formation In Three Shergottite Meteorites From Mars, George Ayeh Anim

Dissertations and Theses

Martian meteorites result from large impact events on the surface of Mars that cause intense shock metamorphism. This study examines the shock metamorphic features of three shergottite meteorites, Dho 019, NWA 12241, and NWA 15628, to better understand shock impact events. The research uses petrographic analysis, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and electron backscatter diffraction (EBSD) to analyze the meteorites. A key focus of this research is the formation of maskelynite, a glass with plagioclase composition, whose formation mechanism has been the substance of debate among researchers. This study proposes that maskelynite forms through a transient intermediary …

Commensality In The Eateries Of Roman Pompeii, Amaya Yolanda Eckersley

Dissertations and Theses

Despite a nearly 300-year excavation history at Pompeii and many identified eating and drinking establishments, social behavior in these public or semi-public spaces has eluded significant research. Elite Roman literature is the traditional guideline for understanding Roman mealtimes and what they meant for social behavior. This literature, however, fails to accurately represent its sub-elite inhabitants. These sub-elites of Rome largely had their meals prepared outside of their homes. Pompeii is no exception and provides a massive boon for researchers willing to consider archaeological evidence. The buildings and evidence buried at Pompeii have ultimately shown a wide array of tabernae related …

Unh Research Team Finds Concentrated Pfas In Watershed Foams, Robbin Ray

Media Relations

No abstract provided.

The Lumberjack, February 19, 2025

Cal Poly Humboldt's Student Newspaper

The student newspaper of Humboldt State University.

Lindenwood Digest, February 19, 2025, Lindenwood University

Lindenwood Digest

The Lindenwood Digest has been a digital employee newsletter since 2009.

Silencing Faf2 Mitigates Alcohol-Induced Hepatic Steatosis By Modulating Lipolysis And Pcsk9 Pathway, Nazmul Huda, Praveen Kusumanchi, Yanchao Jiang, Hui Gao, Themis Thoudam, Ge Zeng, Nicholas J. Skill, Zhaoli Sun, Suthat Liangpunsakul, Jing Ma, Zhihong Yang

School of Medicine Faculty Publications

BACKGROUND: Chronic alcohol consumption leads to lipid accumulation, oxidative stress, cellular damage, and inflammation in the liver, collectively referred to as alcohol-associated liver disease (ALD). FAF2/UBXD8/ETEA (Fas-associated factor 2) is a ubiquitin ligase adaptor protein that plays a crucial role in the ubiquitin-mediated degradation of misfolded proteins in the endoplasmic reticulum. A recent genome-wide association study indicated an association between FAF2 and ALD; however, the exact contribution of FAF2 to ALD pathogenesis remains unclear. METHODS: FAF2 was knocked down using AAV-delivered shRNA in C57/BL6 mice. Mice were subjected to a chronic-plus-single binge ethanol feeding (NIAAA) model. Nine hours after gavage, …

The Antelope, University Of Nebraska At Kearney

The Antelope

No abstract provided.

Study To Understand The Effects Of Biofouling And Corrosion On Underwater Munitions: Implications For Risk Management And Resource Allocation, Center For International Stabilization And Recovery, Fenix Insight

Global CWD Repository

The project's primary objective is to understand the impacts of the underwater environment on munitions as a means to identify the residual risk these munitions pose, so people undertaking detection and clearance efforts can reliably assess operational risk and make informed decisions.

Consumer Acceptance Of Gene-Edited Food: The Role Of Knowledge, Trust, And Information, Anubrata Deka, Syed Imran Ali Meerza, Amalia Yiannaka

Cornhusker Economics

The research aims to assess consumer preferences and attitudes toward CRISPR applications in the food sector and estimate consumer willingness to pay for a food product developed using CRISPR technology to enhance food safety and confer health benefits. Additionally, the research assesses the impact of information provision on perceptions, attitudes and willingness to pay. Specifically, we consider the potential effect of both the nature of information provided (i.e., whether it emphasizes the differences between GM and CRISPR technology or not) and the delivery format (i.e., text versus video) used to present content-equivalent information. In this context, we also assess consumer …

Phylogenetic Allometric Scaling Of Near Basal Breathing Frequency In Terrestrial, Semi-Aquatic And Aquatic Mammals, Andreas Fahlman, Elliot Stielstra, Ethan Wilstermann, Simon Rylaarsdam, Josefin Larsson, Guillermo J. Sanchez -Contreras, Suguru Higa, Gonçalo N. Marques, Malgosia Kaczmarska, Jason Somarelli, Stacy L. De Ruiter

University Faculty Publications and Creative Works

We measured the BASAL breathing frequency following an overnight fast in adult,non-pregnant/non-lactating, inactive mammals ranging in body mass from 15 to5520 kg. The data included results from 338 individual animals from 34 speciesthat were divided into terrestrial, semi-aquatic (Otariidae and Phocidae) and aquaticmammals. Following attempts to limit the collection of breathing frequency usinga basal definition and to correct the analysis phylogenetically, our results suggestthat there are differences in the allometric mass-exponent between terrestrial andaquatic/semi-aquatic mammals. An allometric regression model, whereby both bodymass and breathing frequency were transformed using log10 , suggested that theallometric mass exponent for terrestrial mammals (−0.303) was …

Purdue Stars Performance Analysis, Kayla Gurganus, Lynne Dahmen

Institute for a Sustainable Future Documents

This Sustainability Performance Analysis (SPA) report reviews Purdue’s past and present sustainability performance as captured through STARS reporting to understand sustainability actions and to clearly and objectively identify where Purdue excels and how it might improve performance through the next STARS 3.0 submission.

Unh Research Team Finds Concentrated Pfas In Watershed Foams, Robbin Ray

UNH Today Archive

No abstract provided.

Benefits Of Equilibrium Between Microbiota- And Host-Derived Ligands Of The Aryl Hydrocarbon Receptor After Stroke In Aged Male Mice, Pedram Peesh, Maria P Blasco-Conesa, Ahmad El Hamamy, Romeesa Khan, Gary U Guzman, Parisa Honarpisheh, Eric C Mohan, Grant W Goodman, Justin N Nguyen, Anik Banerjee, Bryce E West, Kyung Ae Ko, Janelle M Korf, Chunfeng Tan, Huihui Fan, Gabriela D Colpo, Hilda Ahnstedt, Lucy Couture, Solji Roh, Julia K Kofler, Jose F Moruno-Manchon, Michael E Maniskas, Jaroslaw Aronowski, Rodney M Ritzel, Juneyoung Lee, Jun Li, Robert M Bryan, Anjali Chauhan, Venugopal Reddy Venna, Louise D Mccullough, Bhanu Priya Ganesh

Faculty, Staff and Student Publications

Recent studies have highlighted the crucial role of microglia (MG) and their interactions with the gut microbiome in post-stroke neuroinflammation. The activation of immunoregulatory pathways, including the aryl hydrocarbon receptor (AHR) pathway, is influenced by a dynamic balance of ligands derived from both the host and microbiota. This study aimed to investigate the association between stroke-induced dysbiosis and the resultant imbalance in AHR ligand sources (loss of microbiota-derived [indole-based] and increase of host-derived [kynurenine-based]) after stroke. Microbiota-derived AHR ligands decreased in human plasma and remained low for days following an ischemic stroke highlighting the translational significance. Transient-middle-cerebral-artery-occlusion was performed in …

Effect Of Multispecies Swards On The Fat Composition And Oxidative Stability Of Milk From Dairy Cows In Mid-Late Lactation, Samuel Rapisarda, Kate M. Mccarthy, Tommy M. Boland, Helen Sheridan, Frank J. Monahan, Finbar J. Mulligan, Graham O'Neill, Nissreen Abu-Ghannam

Articles

Multispecies swards have been recognized as more sustainable than traditional ryegrass systems, in addition to providing a range of phytochemicals to the ruminant diet. In this regard, polyphenols may be linked to higher levels of bioactive fatty acids in milk and enhanced nutraceutical properties in dairy products. This study compares the fat composition and oxidative stability of milk produced by cows fed on multispecies swards (MULTI) with milk from two conventional swards [a perennial ryegrass monoculture (PRG) and a mixture of perennial ryegrass and white clover (PRG + WC)] during the Irish summer. Milk from cows fed on MULTI had …

Mir-125a-3p And Its Targets: Promising Biomarkers For Ovarian, Liver, And Breast Cancers, Offering A Beacon Of Hope For More Effective Diagnosis And Treatment Strategies, Eman Kamaleldien Anwer

Theses and Dissertations

Cancer is a worldwide health problem with various genetic and epigenetic alterations, resulting in disease heterogeneity. Effective diagnostic, therapeutic, and monitoring markers have been determined recently; however, new diagnostic, prognostic, and therapeutic markers are needed. MicroRNAs (miRNA), short non-coding RNAs, have a highlighted role in cancer progression, migration, and programmed cell death. Our study aims to investigate the significance of miR-125a-3p and its downstream targets in breast (BRCA), hepatocellular (HCC), and ovarian (OVCA) malignancies and their possibility as diagnostic biomarkers and targets for therapeutic agents. Bioinformatics and online tools were used to determine the miRNA-predicted downstream targets, analyze GEO datasets, …

Identification Of Novel Argonaute Proteins Using A Metagenomic Mining Approach, Lobna Abdallah Ghonaim

Theses and Dissertations

Gene editing is one of the most promising tools in science. It enables precise modifications of an organism's genetic material. Metagenomics is considered a powerful tool that unlocks the broad genetic potential found in uncultured microbial communities. Exploring the genetic diversity of uncultured microbial communities helps identify novel functional proteins with unique properties and make the best use of these diverse microbial ecosystems.

We developed and employed a metagenomic-based approach to mine more than 1000 metagenomes for prokaryotic argonaute proteins (pAgos), a potential gene editing machinery encoded in bacterial and archaeal genomes. Our workflow involved strict quality control, sequence assembly, …