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Articles 16261 - 16290 of 23207
Full-Text Articles in Entire DC Network
Urban Agriculture Technical Advisory, Katherine Rose Ivanjack, Kylie J. Preciado
Urban Agriculture Technical Advisory, Katherine Rose Ivanjack, Kylie J. Preciado
City and Regional Planning
This project serves as a senior capstone, aimed at exploring the potential of urban agriculture to address critical challenges in sustainability, food security, and community development within California cities. This project highlights practical and innovative approaches to urban farming that can be adopted or adapted to suit the unique needs of urban areas across the state.
The Adoption Of The Braided River Model Toward An Inclusive Stem Workforce For All, Aixa Alemán-Díaz, Sakib Hussen, Abdul Siam, Candice M. Etson, Robin Mc C. Greenler, Taylor Lightner, Semarhy Quiñones-Soto, Simone B. Soso, Verónica A. Segarra
The Adoption Of The Braided River Model Toward An Inclusive Stem Workforce For All, Aixa Alemán-Díaz, Sakib Hussen, Abdul Siam, Candice M. Etson, Robin Mc C. Greenler, Taylor Lightner, Semarhy Quiñones-Soto, Simone B. Soso, Verónica A. Segarra
Physics: Faculty Publications
Despite decades of interventions aiming to transform the science, technology, engineering, and mathematics (STEM) workforce to be more inclusive and diverse, little progress has been made in creating long-lasting, sustainable change. For a long period of time, the STEM workforce has been described as a leaky pipeline. While there has been some utility to thinking about the STEM workforce in this way, in this article, we discuss how characterizing the STEM workforce as a leaky pipeline can impede the design of innovative interventions that contribute to sustainable change toward a more inclusive scientific enterprise. As an alternative, we join others …
Applications Of Archaeal Antioxidant Coenzyme M As An Agricultural Biostimulant, Jeremy Hunter Brown
Applications Of Archaeal Antioxidant Coenzyme M As An Agricultural Biostimulant, Jeremy Hunter Brown
Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–
As the demand to meet global food security goals continue to increase, the need to increase efficiency and productivity of agricultural output also rises. This calls for innovative and cost-effective methods for improving plant growth and yield. Here we explore how antioxidants, molecules that inhibit oxidation and are necessary for redox based signaling and redox homeostasis, can be utilized as chemical biostimulants. Specifically, we investigated Coenzyme M, an antioxidant from archaea that holds potential economic advantages over other antioxidants. Chapter 1 provides a comprehensive review of antioxidants and how exogenously application of them affects plant growth and biology, highlighting the …
Emoji Marketing: Toward A Theory Of Brand Paralinguistics, Jacob Almaguer, Reto Felix, Colleen M. Harmeling
Emoji Marketing: Toward A Theory Of Brand Paralinguistics, Jacob Almaguer, Reto Felix, Colleen M. Harmeling
Marketing Faculty Publications
Emojis, or pictographs that supplement or replace written language, have become ubiquitous in contemporary communication, including emoji marketing. Drawing on insights from linguistics and sign theory, the current research proposes an emoji marketing framework in which emoji symbolism (symbolic vs. iconic emoji use) affects consumers’ message appraisals (perceived message intimacy and clarity), which in turn influence brand cultural relevance (propositions P1 and P2). Emoji syntax (i.e., whether emojis are supplemented with text or not) and marketer-consumer group relatedness (shared vs. unshared group membership) moderate the relationship between emoji symbolism and consumers’ message appraisals. The framework suggests that messages that use …
March 2025, Office Of Sustainability
March 2025, Office Of Sustainability
Office of Sustainability Newsletter
No abstract provided.
Influence Of Nitrogen And Phosphorus Rates On The Growth Of Water Hyacinth (Pontederia Crassipes Mart.), Mary Joy M. Abit, Rene Franchesca A. Pilar, Analiza Henedina M. Ramirez
Influence Of Nitrogen And Phosphorus Rates On The Growth Of Water Hyacinth (Pontederia Crassipes Mart.), Mary Joy M. Abit, Rene Franchesca A. Pilar, Analiza Henedina M. Ramirez
The Philippine Agricultural Scientist
Research has been conducted globally to investigate the growth and colonization patterns of Pontederia crassipes Mart. This research aimed to examine the impact of varying rates of nitrogen (N) and phosphorus (P) on the growth of P. crassipes and determine the nutrient levels that would result in the greatest growth response. P. crassipes plants were placed in plastic containers filled with 5 L of distilled water. Nitrogen and phosphorus were applied separately at varying concentrations. Specifically, N was added at 0, 10, 20, 30, 40, or 50 mg L-1 and P at 0, 1, 3, 5, 7, or 9 …
Implementation Of Organic Rice Farming Based On Local Wisdom In Bali Province, Indonesia, Gede Sedana, Ketut Wirawan, Naori Miyazawa
Implementation Of Organic Rice Farming Based On Local Wisdom In Bali Province, Indonesia, Gede Sedana, Ketut Wirawan, Naori Miyazawa
The Philippine Agricultural Scientist
The Green Revolution has significantly contributed to agricultural development. However, it has also caused environmental damage due to excessive use of agricultural inputs and land exploitation. To promote sustainability in line with the Sustainable Development Goals (SGDs), organic farming has been recommended to improve and maintain agroecosystems. In Bali Province, Indonesia, the concept of Tri Hita Karana (THK), a form of local wisdom, has guided farmers in implementing the System of Rice Intensification (SRI) technique introduced by the government. This study explored how organic rice farming integrates local wisdom, assessed farmers’ knowledge and attitudes towards SRI, and examined government support …
Evaluate A Personal-Sized Seedless Watermelon Grown In High Tunnels, Wenjing Guan
Evaluate A Personal-Sized Seedless Watermelon Grown In High Tunnels, Wenjing Guan
Midwest Vegetable Trial Reports
Growing personal-sized seedless watermelons in high tunnels is not a common practice in the U.S. However, as high tunnel adoption increases and farmers gain experience in using them for specialty crop production, there is a need to evaluate nontraditional crops to improve crop rotation within these structures. In this study, we evaluated the performance of a personal-sized seedless watermelon grown in a high tunnel system. This seedless watermelon cultivar was selected because of its thin rind, tender flesh, and high sugar content. The combination of these fruit traits may make it particularly attractive for local food markets. Additionally, we compared …
The Role Of Mthfr Gene Mutation In Neurodevelopmental Disorders: Exploring Potential Correlations With Attention Deficit Hyperactivity Disorder And Anxiety, Heba Mehmood
All NMU Master's Theses
One of the most common neurodevelopmental disorders is Attention Deficit Hyperactivity Disorder (ADHD), which is often co-occurring with symptoms of depression and anxiety. The misdiagnosis and overdiagnosis of ADHD is agreed to be a prevalent problem. The methylenetetrahydrofolate reductase (MTHFR) gene has been identified as a possible contributor to ADHD. The goal of this research is to decrease the frequency at which ADHD is misdiagnosed by investigating the correlation between the MTHFR gene at the single nucleotide polymorphism (SNP) C677T to ADHD and anxiety. A group of 80 participants’ DNA samples were collected through cheek swabs following IRB approval. Participants …
Occlusion-Insensitive Talking Head Video Generation Via Facelet Compensation, Yuhui Deng, Yuqin Lu, Yangyang Xu, Yongwei Nie, Shengfeng He
Occlusion-Insensitive Talking Head Video Generation Via Facelet Compensation, Yuhui Deng, Yuqin Lu, Yangyang Xu, Yongwei Nie, Shengfeng He
Research Collection School Of Computing and Information Systems
Talking head video generation involves animating a still face image using facial motion cues derived from a driving video to replicate target poses and expressions. Traditional methods often rely on the assumption that the relative positions of facial keypoints remain unchanged. However, this assumption fails when keypoints are occluded or when the head is in a profile pose, leading to inconsistencies in identity and blurring in certain facial regions. In this paper, we introduce Occlusion-Insensitive Talking Head Video Generation, a novel approach that eliminates the reliance on spatial correlation of keypoints and instead leverages semantic correlation. Our method transforms facial …
Improving Multimodal Human Pose Estimation By Adversarial Modality Enhancement, Jiangnan Xia, Qilong Wu, Yanyin Guo, Yi Li, Jianghan Cheng, Junwei Li, Zhiyuan Zhang
Improving Multimodal Human Pose Estimation By Adversarial Modality Enhancement, Jiangnan Xia, Qilong Wu, Yanyin Guo, Yi Li, Jianghan Cheng, Junwei Li, Zhiyuan Zhang
Research Collection School Of Computing and Information Systems
Human pose estimation in computer vision predominantly focuses on the visible modality, with limited research on the infrared modality. No existing methods demonstrate robust performance across both modalities, missing their complementary strengths. This gap arises from the lack of a multimodal benchmark and the difficulty of developing robust multimodal capabilities. To address this, we introduce MMPD, a novel visible-infrared multimodal pose benchmark with high-quality annotations for both modalities. Leveraging MMPD, we expose the limitations of state-of-the-art methods due to modality variance. To overcome this challenge, we propose a novel method-agnostic scheme called AMMPE. By employing the Modality Adversarial Enhancement Stage …
Environmental Costs Of The West Philippine Sea Dispute In Light Of Catholic Social Teaching, Teofilo Giovan S. Pugeda Iii
Environmental Costs Of The West Philippine Sea Dispute In Light Of Catholic Social Teaching, Teofilo Giovan S. Pugeda Iii
Loyola Papers
This paper is an analytical reflection on the insights Catholic Social Teaching (CST) has to offer on the West Philippine Sea Dispute. It discusses (1) an overview of the maritime dispute, mainly from the Philippine perspective, (2) the disputed legal status of the West Philippine Sea in local and international law, (3) the state of coral reefs in the South China Sea, particularly in the West Philippine Sea, (4) CST on the environment, especially concerning water, and (5) some practical and conceptual avenues to enrich the conversation.
Ukrainian Internally Displaced Persons And Their Journey Of Christian Hope, Pamela A. Tuason
Ukrainian Internally Displaced Persons And Their Journey Of Christian Hope, Pamela A. Tuason
Loyola Papers
This paper examines eighteen narratives of Ukrainian internally displaced persons (IDPs) during the ongoing war and interprets them through the lens of Christian hope as articulated by Jürgen Moltmann. Using Paul Ricoeur’s threefold mimetic theory—prefiguration, configuration, and refiguration—the study analyzes how personal stories reveal theological meanings embedded in experiences of suffering, trauma, and displacement. The narratives highlight the role of history in displacement, the capacity of traumatized persons to reinterpret suffering through hope, and the longing to regain home. The study also proposes a model for the theological study of migrant narratives, contributing to migration theology and to discussions on …
Spotting And Responding To Health Misinformation And Disinformation, Michael J. Mcgee, Jean-Philippe Berteau
Spotting And Responding To Health Misinformation And Disinformation, Michael J. Mcgee, Jean-Philippe Berteau
Open Educational Resources
Three one-hour lessons. In these lessons students will learn to identify health misinformation which spreads rapidly through social media, search engines, and online platforms. By generating false health news stories with AI and critically analyzing them, students will develop essential skills for public health literacy and ethical responsibility. These activities equip future health professionals with the tools to discern evidence-based information from deceptive content and provide guidance to ensure accurate and authentic communication in the field.
Pigs Get Fat, Hogs Get Slaughtered: Why The Court Over-Indulged With The Pike Balancing Test And How Shedding The Excess Can Save The Dormant Commerce Clause, Jacob Redden
University of Dayton Law Review
No abstract provided.
Real Concerns For An Artificial Threat: Artists, Ai, And The Battle To Script Hollywood’S Future, Evan Sommer
Real Concerns For An Artificial Threat: Artists, Ai, And The Battle To Script Hollywood’S Future, Evan Sommer
Nevada Law Journal
No abstract provided.
Exploring Oxylipins In Host–Microbe Interactions And Their Impact On Infection And Immunity, Robert J. Neff, Christopher D. Radka
Exploring Oxylipins In Host–Microbe Interactions And Their Impact On Infection And Immunity, Robert J. Neff, Christopher D. Radka
Markey Cancer Center Faculty Publications
Plasma lipids are essential components of biological systems, transported through interactions with proteins to maintain cellular functions. These lipids exist in various forms, such as fatty acids, glycerolipids, glycerophospholipids, sphingolipids, sterols, and prenol lipids, derived from dietary intake, adipose tissue, and biosynthesis. While the association between certain fatty acids and cardiovascular diseases has been widely recognized, polyunsaturated fatty acids (PUFAs) exhibit cardioprotective effects, reducing risks of arrhythmias and heart-related mortality. This is due to their role in the production of eicosanoids, which modulate inflammation. Chronic inflammation, particularly in obesity, is significantly influenced by fatty acids, with saturated fatty acids promoting …
Characterization, Performance, And Toxicological Assessment Of Polysulfone-Sulfonated Polyether Ether Ketone Membranes For Water Separation Applications, Muhammad Usman Yousaf, Lucca Madeo Cortarelli, Nerissa I. Jebet, Jason M. Unrine, Nirupam Aich, Olga V. Tsyusko, Isabel C. Escobar
Characterization, Performance, And Toxicological Assessment Of Polysulfone-Sulfonated Polyether Ether Ketone Membranes For Water Separation Applications, Muhammad Usman Yousaf, Lucca Madeo Cortarelli, Nerissa I. Jebet, Jason M. Unrine, Nirupam Aich, Olga V. Tsyusko, Isabel C. Escobar
Markey Cancer Center Faculty Publications
The removal of small molecular weight charged compounds from aqueous solutions using membrane remains a challenge. In this study, polysulfone (PSf)- and sulfonated polyether ether ketone (SPEEK)-based membranes were fabricated via non- solvent induced phase separation process (NIPS) using N-Methyl-2-Pyrrolidone (NMP) as solvent and water as non-solvent. Membranes were characterized structurally and morphologically, followed by toxicity assessment conducted before and after filtration, both with and without annealing at various pH values to evaluate potential leaching of trapped solvent from the membrane pores. Additionally, membrane performance was characterized using binary mixtures of cationic and anionic dyes. The results demonstrated selective filtration …
Computational Design And In Vitro And In Vivo Characterization Of An Apoe-Based Synthetic High-Density Lipoprotein For Sepsis Therapy, Ling Guo, Yaxia Yuan, Fang Zhang, Chang-Guo Zhan, Xiangan Li
Computational Design And In Vitro And In Vivo Characterization Of An Apoe-Based Synthetic High-Density Lipoprotein For Sepsis Therapy, Ling Guo, Yaxia Yuan, Fang Zhang, Chang-Guo Zhan, Xiangan Li
Markey Cancer Center Faculty Publications
Introduction: Septic patients have low levels of high-density lipoproteins (HDLs), which is a risk factor. Replenishing HDLs with synthetic HDLs (sHDLs) has shown promise as a therapy for sepsis. This study aimed to develop a computational approach to design and test new types of sHDLs for sepsis treatment. Methods: We used a three-step computational approach to design sHDL nanoparticles based on the structure of HDLs and their binding to endotoxins. We tested the efficacy of these sHDLs in two sepsis mouse models—cecal ligation and puncture (CLP)-induced and P. aeruginosa-induced sepsis models—and assessed their impact on inflammatory signaling in cells. Results: …
My Apple Journal And Reflection Entries Are Being Used To Train An Ai That Knows My Emotions Before I Do And That's Ok, Andrew Zhang, Special Collections, Fleet Library
My Apple Journal And Reflection Entries Are Being Used To Train An Ai That Knows My Emotions Before I Do And That's Ok, Andrew Zhang, Special Collections, Fleet Library
12th Baker & Whitehill Student Artists' Book Contest 2026
Entry for the 12th Baker & Whitehill Student Artists' Book Contest. Opening reception and award ceremony Wednesday, February 25, 2026 at 6:30pm, Fleet Library, 1st Floor Main Reading Room. Juror: Gabrielle Reed.
Research On The Mechanism Of China's Stock Market And The Efficiency Of Factor-Based Stock Selection Strategies, Huaixiang Xing
Research On The Mechanism Of China's Stock Market And The Efficiency Of Factor-Based Stock Selection Strategies, Huaixiang Xing
Dissertations and Theses Collection (Open Access)
Based on the data of 4280 firms listed on China's A-share market from 2010 to 2023, this paper constructs 20 market factors and 4 financial factors to systematically study multi factor stock selection strategies in China's stock market. Furthermore, this article analyzes the impact of China's capital market mechanisms on stock selection strategies, which include margin trading and securities lending mechanism, Mainland-Hong Kong Stock Connect mechanism, analyst coverage mechanism, and information disclosure mechanism. The main conclusions are as follows:
Firstly, this paper reveals that 12 market factors, including size, price, and liquidity are all effective pricing factors, and the strategy …
Research On The Economic Performance And Process Optimization Strategies In Chinese Agricultural Enterprises, Zili Xu
Dissertations and Theses Collection (Open Access)
With the booming digital economy, digital transformation has become a key strategy for global enterprises to enhance competitiveness and optimize economic performance. This dissertation takes Chinese agricultural enterprises as the research subject and deeply explores the impact of digital transformation on corporate economic performance and strategies for business process optimization.
The dissertation first reviews the research progress on the digital transformation of agricultural enterprises. Secondly, it uses qualitative text analysis and machine learning technology to assess the digital level of Chinese agricultural enterprises and analyzes their current status and characteristics. The empirical study employs a fixed-effects model and finds that …
Regulating Hidden Ai Authorship, Jacob Noti-Victor
Regulating Hidden Ai Authorship, Jacob Noti-Victor
Articles
With the rapid emergence of high-quality generative artificial intelligence (“AI”), some have advocated for mandatory disclosure when the technology is used to generate new text, images, or video. But the precise harms posed by nontransparent uses of generative AI have not been fully explored. While the use of the technology to produce material that masquerades as factual (“deepfakes”) is clearly deceptive, this Article focuses on a more ambiguous area: the consumer’s interest in knowing whether works of art or entertainment were created using generative AI. In the markets for creative content—fine art, books, movies, television, music, and the like—producers have …
Functional Investigation Of A Putative Calcium-Binding Site Involved In The Inhibition Of Inositol 1,4, 5-Trisphosphate Receptor Activity, Vikas Arige, Larry E Wagner, Sundeep Malik, Mariah R Baker, Guizhen Fan, Irina I Serysheva, David I Yule
Functional Investigation Of A Putative Calcium-Binding Site Involved In The Inhibition Of Inositol 1,4, 5-Trisphosphate Receptor Activity, Vikas Arige, Larry E Wagner, Sundeep Malik, Mariah R Baker, Guizhen Fan, Irina I Serysheva, David I Yule
Faculty, Staff and Student Publications
The regulation of inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) activity is thought to define the spatiotemporal patterns of Ca2+ signals necessary for the appropriate activation of downstream effectors. The binding of both IP3 and Ca2+ is obligatory for IP3R channel opening. Ca2+ however regulates IP3R activity in a biphasic manner. Ca2+ binding to a high-affinity pocket formed by the third armadillo repeat domain and linker domain promotes IP3R channel opening without altering the Ca2+ dependency for channel inactivation. These data suggest that a distinct low-affinity Ca2+-binding site is responsible for the reduction in IP3R activity at higher [Ca2+]. We mutated a …
Disrupting Tymp-Mediated Pathways To Block Lymph Node Metastasis In Ovarian Cancer, Emine Bayraktar
Disrupting Tymp-Mediated Pathways To Block Lymph Node Metastasis In Ovarian Cancer, Emine Bayraktar
Dissertations and Theses (Open Access)
Historically, ovarian cancer (OC) was thought to metastasize by surface-to-surface spread, but recent developments have yielded a new understanding of the paths of metastatic spread. In epithelial ovarian cancer, the mechanisms driving lymph node (LN) metastasis are poorly understood, and it remains unclear whether omental metastasis occurs in parallel with LN metastasis. Utilizing comparative transcriptomic and proteomic profiling of primary tumors, LN metastases, and omental metastases, we identified significant upregulation of thymidine phosphorylase (TYMP) in LN metastases compared to primary and omental tumor sites. Functional studies in murine models demonstrated that TYMP silencing significantly suppressed LN metastasis, underscoring TYMP as …
Race, The Academy, And The Constitution Of The War On Drugs, Jeffrey Bellin, Bennett Capers
Race, The Academy, And The Constitution Of The War On Drugs, Jeffrey Bellin, Bennett Capers
Vanderbilt Law School Faculty Publications
The war on drugs is widely viewed as a policy failure. Despite massive government intrusions on personal liberty, drug addiction, overdoses, and drug-related violence have only in- creased since the war was declared in 1971. David Pozen's new book, The Constitution of the War on Drugs, reveals a constitutional failure as well. Pozen chronicles a host of constitutional arguments that American litigants deployed to protect a "right" to use drugs with surprising, if fleeting, suc- cess. Pozen asks what might have been, exploring why the courts backtracked and effectively re- moved the Constitution as a meaningful obstacle to drug prohibitions. …
Policing’S Free-Speech Problem, G. Alex Sinha
Policing’S Free-Speech Problem, G. Alex Sinha
Utah Law Review
The central claim of this Article is that a significant share of typical policing activity is wildly and egregiously unconstitutional. More precisely, police regularly, predictably, and systematically violate the hardest, most settled core of free-speech law under the First Amendment. We have grown to tolerate these violations—we have not even noticed them!—because they are difficult to litigate. But they are inescapable. Having seen them, we cannot abide these violations without entirely overturning the modern understanding of free-speech rights under the First Amendment.
It is well-settled that, under the First Amendment, the government generally may not discriminate against (or in favor …
Sacubitril/Valsartan Attenuates Inflammation And Myocardial Fibrosis In Takotsubo-Like Cardiomyopathy, Jiangying Kuang, Zhiyi Jia, Tou Kun Chong, Jian Chen, Kan Liu, Xin Wang, Zhaohua Li, Jing Zhang, Yanru Kong, Lin Deng, Martin Cadieras, Yuanyuan Sun, Rong Sun, Qinghua Lu, Yusheng Liu
Sacubitril/Valsartan Attenuates Inflammation And Myocardial Fibrosis In Takotsubo-Like Cardiomyopathy, Jiangying Kuang, Zhiyi Jia, Tou Kun Chong, Jian Chen, Kan Liu, Xin Wang, Zhaohua Li, Jing Zhang, Yanru Kong, Lin Deng, Martin Cadieras, Yuanyuan Sun, Rong Sun, Qinghua Lu, Yusheng Liu
2020-Current year OA Pubs
BACKGROUND: Takotsubo syndrome (TTS) primarily manifests as a cardiomyopathy induced by physical or emotional stress, remains a poorly understood condition with no established treatments. In this study, we investigated the potential of sacubitril/valsartan (sac/val) to increase the survival of TTS patients and reduce inflammation and myocardial fibrosis in experimental models.
AIM: This study aimed to evaluate whether sac/val could improve survival rates in TTS patients, mitigate cardiac remodeling in vivo, and explore its anti-inflammatory and antifibrotic mechanisms in vitro.
METHODS: Clinical cases from the Chinese Takotsubo syndrome (ChiTTS) registry were analyzed to assess patient survival rates. In addition, we used …
Unveiling Vaginal Fibrosis: A Novel Murine Model Using Bleomycin And Epithelial Disruption, Jennifer M Mccracken, Gisele A Calderon, Felipe Rivas, Dorothea Erxleben, Taylor Moseley, Lishore A Kumar, Daniel E Kennedy, Swathi Balaji, Adam Hall, Julie C E Hakim
Unveiling Vaginal Fibrosis: A Novel Murine Model Using Bleomycin And Epithelial Disruption, Jennifer M Mccracken, Gisele A Calderon, Felipe Rivas, Dorothea Erxleben, Taylor Moseley, Lishore A Kumar, Daniel E Kennedy, Swathi Balaji, Adam Hall, Julie C E Hakim
Faculty, Staff and Students Publications
Vaginal fibrosis induced by cancer therapy continues to take a physical and psychoemotional burden. To advance the efforts to generate a reproducible and cost-effective animal model, we tested the effect of repeated bleomycin instillations with mucosal layer disruption on induction of vaginal fibrosis. Tissue samples collected at various time points were analyzed for fibrosis-related gene expression changes and collagen content. Low (1.5 U/kg) and high-dose (2.5 U/kg) bleomycin instillations alone did not induce fibrosis, but when high-dose bleomycin was combined with epithelial disruption, increased profibrotic gene expression and trichrome staining were observed. To evaluate spatial and temporal changes in the …
Genetically Engineered Brain Organoids Recapitulate Spatial And Developmental States Of Glioblastoma Progression, Matthew Ishahak, Rowland H Han, Devi Annamalai, Timothy Woodiwiss, Colin Mccornack, Ryan T Cleary, Patrick A Desouza, Xuan Qu, Sonika Dahiya, Albert H Kim, Jeffrey R Millman
Genetically Engineered Brain Organoids Recapitulate Spatial And Developmental States Of Glioblastoma Progression, Matthew Ishahak, Rowland H Han, Devi Annamalai, Timothy Woodiwiss, Colin Mccornack, Ryan T Cleary, Patrick A Desouza, Xuan Qu, Sonika Dahiya, Albert H Kim, Jeffrey R Millman
2020-Current year OA Pubs
Glioblastoma (GBM) is an aggressive form of brain cancer that is highly resistant to therapy due to significant intra-tumoral heterogeneity. The lack of robust in vitro models to study early tumor progression has hindered the development of effective therapies. Here, this study develops engineered GBM organoids (eGBOs) harboring GBM subtype-specific oncogenic mutations to investigate the underlying transcriptional regulation of tumor progression. Single-cell and spatial transcriptomic analyses revealed that these mutations disrupt normal neurodevelopment gene regulatory networks resulting in changes in cellular composition and spatial organization. Upon xenotransplantation into immunodeficient mice, eGBOs form tumors that recapitulate the transcriptional and spatial landscape …