Digital Commons Network^™

Dlk-Dependent Axonal Mitochondrial Fission Drives Degeneration After Axotomy, Jorge Gómez-Deza, Matthew Nebiyou, Mor R Alkaslasi, Francisco M Nadal-Nicolás, Preethi Somasundaram, Anastasia L Slavutsky, Wei Li, Michael E Ward, Trent A Watkins, Claire E Le Pichon

Faculty, Staff and Students Publications

Currently there are no effective treatments for an array of neurodegenerative disorders to a large part because cell-based models fail to recapitulate disease. Here we develop a reproducible human iPSC-based model where laser axotomy causes retrograde axon degeneration leading to neuronal cell death. Time-lapse confocal imaging revealed that damage triggers an apoptotic wave of mitochondrial fission proceeding from the site of injury to the soma. We demonstrate that this apoptotic wave is locally initiated in the axon by dual leucine zipper kinase (DLK). We find that mitochondrial fission and resultant cell death are entirely dependent on phosphorylation of dynamin related …

Orthogonal And Multiplexable Genetic Perturbations With An Engineered Prime Editor And A Diverse Rna Array, Qichen Yuan, Hongzhi Zeng, Tyler C Daniel, Qingzhuo Liu, Yongjie Yang, Emmanuel C Osikpa, Qiaochu Yang, Advaith Peddi, Liliana M Abramson, Boyang Zhang, Yong Xu, Xue Gao

Faculty, Staff and Students Publications

Programmable and modular systems capable of orthogonal genomic and transcriptomic perturbations are crucial for biological research and treating human genetic diseases. Here, we present the minimal versatile genetic perturbation technology (mvGPT), a flexible toolkit designed for simultaneous and orthogonal gene editing, activation, and repression in human cells. The mvGPT combines an engineered compact prime editor (PE), a fusion activator MS2-p65-HSF1 (MPH), and a drive-and-process multiplex array that produces RNAs tailored to different types of genetic perturbation. mvGPT can precisely edit human genome via PE coupled with a prime editing guide RNA and a nicking guide RNA, activate endogenous gene expression …

Age-Dependent Regulation Of Axoglial Interactions And Behavior By Oligodendrocyte Ankyring, Xiaoyun Ding, Yu Wu, Anna Vainshtein, Victoria Rodriguez, Emily Ricco, James T Okoh, Yanhong Liu, Daniel C Kraushaar, Elior Peles, Matthew N Rasband

Faculty, Staff and Students Publications

The bipolar disorder (BD) risk gene ANK3 encodes the scaffolding protein AnkyrinG (AnkG). In neurons, AnkG regulates polarity and ion channel clustering at axon initial segments and nodes of Ranvier. Disruption of neuronal AnkG causes BD-like phenotypes in mice. During development, AnkG is also expressed at comparable levels in oligodendrocytes and facilitates the efficient assembly of paranodal junctions. However, the physiological roles of glial AnkG in the mature nervous system, and its contributions to BD-like phenotypes, remain unexplored. Here, we show that oligodendroglia-specific AnkG conditional knockout results in destabilization of axoglial interactions in aged but not young adult mice. In …

Transition To Enteral Triazole Antifungal Therapy For Pediatric Invasive Candidiasis: Secondary Analysis Of A Multicenter Cohort Study Conducted By The Pediatric Fungal Network, Robert F T Bucayu, Craig L K Boge, Inci Yildirim, Martha Avilés-Robles, Surabhi B Vora, David M Berman, Tanvi S Sharma, Lillian Sung, Elio Castagnola, Debra L Palazzi, Lara Danziger-Isakov, Dwight E Yin, Emmanuel Roilides, Gabriela Maron, Alison C Tribble, Pere Soler-Palacin, Eduardo López-Medina, José Romero, Kiran Belani, Antonio C Arrieta, Fabianne Carlesse, Dawn Nolt, Natasha Halasa, Daniel Dulek, Sujatha Rajan, William J Muller, Monica I Ardura, Alice Pong, Blanca E Gonzalez, Christine M Salvatore, Anna R Huppler, Catherine Aftandilian, Mark J Abzug, Arunaloke Chakrabarti, Michael Green, Irja Lutsar, Elizabeth D Knackstedt, Sarah K Johnson, William J Steinbach, Brian T Fisher, Rachel L Wattier

Faculty, Staff and Students Publications

Of 319 children with invasive candidiasis, 67 (21%) transitioned from intravenous to enteral antifungal therapy. Eight (12%) transitioned back to intravenous antifungal therapy, one due to perceived treatment failure defined by clinical progression or worsening. Global treatment response at study completion was successful in 66 participants who transitioned to enteral therapy.

Food For Thought: A Qualitative Assessment Of Medical Trainee And Faculty Perceptions Of Nutrition Education, Olivia Kunitsky, Mahdi Taye, Karla Feeley, Hugh Johnson, Abigail Glynn, Avery Stivale, Matthew Hamers, Alexis Notarianni, Sireesha Mamillapalli, Gabi Waite, Sonia Lobo

Department of Neurology Faculty Papers

BACKGROUND: The American Society of Clinical Nutrition recommends 37 to 44 h of undergraduate medical nutrition education. The Total Health Curriculum at Geisinger Commonwealth School of Medicine (GCSOM) contains 14 h of objective-based nutritional instruction. This study aimed to examine the perceptions of key stakeholders regarding the role of nutrition in medicine and to identify barriers, opportunities for improvement, and roles/responsibilities for innovative implementation of nutrition education.

METHODS: This exploratory, qualitative study employed a phenomenological approach and inductive coding process. Purposive sampling recruited medical students, undergraduate medical education (UME) faculty, physicians, and other healthcare professionals at GCSOM and affiliated clinical …

No One Lives In The Future: Family Dynamics And Environmental Discourse, Morgan Vanek

ABO: Interactive Journal for Women in the Arts, 1640–1830

In the context of catastrophic climate change, the optimism that underpins our economic models for calculating the future cost of present risks has become both untenable and dangerous. Nonetheless, even writing urgently concerned with these risks continues to invoke a version of the Child as a figure of futurity that appears to naturalize both this optimism and our present inaction. In this essay, I argue that we might find another path forward by looking back to the eighteenth-century stories that seem to demonstrate a similar preoccupation with children in danger. Taking Hogarth’s A Harlot’s Progress as a representative example, I …

Reading As Lesbian, Reading As Trans* And Thinking Intergenerationally, Katherine Binhammer

ABO: Interactive Journal for Women in the Arts, 1640–1830

Written from the perspective of a critic who came of age in the queer 1990s, this essay considers the generational difference in understandings of Charlotte Charke as a butch lesbian and a transman. It argues that the history of sexuality demands an intergenerational reading practice because our objects are always engaged in a history of the present.

Periodizing The Long Eighteenth Century — Across Generations, Katarina O'Briain

ABO: Interactive Journal for Women in the Arts, 1640–1830

This paper approaches the topic of intergenerational thinking by asking it what it might mean to stretch the conventional periodization of the long eighteenth century across generations. Reading Phillis Wheatley’s heroic couplet poems alongside Earl Wasserman’s mid-twentieth-century work on neoclassical poetics, it explores some of the surprising possibilities unleashed by revisiting the work of earlier critics. This mid-twentieth-century scholarship, the essay suggests, establishes a mode of eighteenth-century poetics that lends itself to more recent understandings of abolition and structural change. Earlier generations of critics trace an eighteenth-century poetic theory that resonates in the present moment; many generations before that, Phillis …

Ancestors In The Archives: Decolonizing Archival Research, Willow White

ABO: Interactive Journal for Women in the Arts, 1640–1830

Bringing together my identities as an Indigenous woman (Métis Nation of Alberta) and as a scholar specializing in eighteenth-century women writers has involved the continual unlearning of colonial research practices. The following essay reflects on my efforts to work with/in archives in a decolonizing way. I will share some of the practices that I have adopted, not to provide a checklist, but to join the ongoing conversation of many eighteenth-century studies scholars who share a decolonizing desire.

Reading With Aunt Bunty: Intergenerational Feminism And Eighteenth-Century Studies, Alison Conway

ABO: Interactive Journal for Women in the Arts, 1640–1830

This essay asks: what might an intergenerational reading practice look like for feminist critics working in eighteenth-century studies? What could be learned from reading the political and institutional conditions that have shaped reading practices, past and present, in relation to each other? I consider, here, the case of Q.D. Leavis. Reading Leavis, as well as other women who studied at Cambridge in the early twentieth century, shows me how profoundly I have been shaped by Virginia Woolf’s characterization of the university and literary history’s marginalization of women writers. What would it mean to consider the eighteenth century through the lens …

Introduction: Intergenerational Thinking, Katherine Binhammer

ABO: Interactive Journal for Women in the Arts, 1640–1830

This introduction to a conversation on Intergenerational Thinking that first began at the American Society of Eighteenth Century Studies in Toronto, March 2024, establishes the importance of dialogue across generations in feminist eighteenth-century studies. It synthesizes the methods the participants engage to think with past scholars, rather than disavow such work. While the topics discussed are diverse -- feminist history, Phillis Wheatley, Indigenous eighteenth-century archives, climate catastrophe and Charlotte Charke – the writers share an approach to generational thinking grounded in a critique of the assumption that the new is always better.

A Review Of Kristin M. Girten's Sensitive Witnesses: Feminist Materialism In The British Enlightenment, Peter West

ABO: Interactive Journal for Women in the Arts, 1640–1830

A review of Sensitive Witnesses by Kristin M. Girten by Peter West.

A Review Of Glenn Mcgillivray's Actors, Audiences, And Emotions In The Eighteenth Century: Communities Of Sentiment, Semane Parsons

ABO: Interactive Journal for Women in the Arts, 1640–1830

A Review of Glen McGillivray's book Actors, Audiences, and Emotions in the Eighteenth Century: Communities of Sentiment (2023).

A Review Of Jennifer Maclure’S The Feeling Of Letting Die: Necroeconomics And Victorian Fiction, Jolene Zigarovich

ABO: Interactive Journal for Women in the Arts, 1640–1830

A review of Jennifer MacLure’s The Feeling of Letting Die: Necroeconomics and Victorian Fiction by Jolene Zigarovich.

A Review Of Maeve E. Kane's Shirts Powdered Red: Haudenosaunee Gender, Trade, And Exchange Across Three Centuries, Melissa Adams-Campbell

ABO: Interactive Journal for Women in the Arts, 1640–1830

A review of Shirts Powdered Red: Haudenosaunee Gender, Trade, and Exchange across Three Centuries, by Maeve Kane

“Strange And Unusual Things”: Teaching The Speculative Fiction Of Margaret Cavendish, E Mariah Spencer

ABO: Interactive Journal for Women in the Arts, 1640–1830

Margaret Cavendish, a prolific author and natural philosopher, defies categorization with her multifaceted contributions to literature and philosophy. While she is recognized for her groundbreaking work in various academic fields, her literature also resonates within the realms of science fiction, weird fiction, and speculative genres. This essay explores innovative approaches to teaching Cavendish's work, particularly within the realm of speculative literature, using an interdisciplinary lens. Drawing on perspectives including monster theory and the concept of the abject, students are prompted to critically engage with Cavendish's texts. By examining themes such as the monstrous, the supernatural, and the occult, students uncover …

Teaching Margaret Cavendish Through The Lens Of Scientific Anti-Realism, Kevin Lower

ABO: Interactive Journal for Women in the Arts, 1640–1830

Margaret Cavendish (1623-1673) only began receiving sustained attention by historians of philosophy in recent decades, but she is being rapidly integrated into the early modern philosophical canon. This essay explores one dimension of teaching Cavendish that presents difficulties for introductory philosophy students: her criticisms of experimental philosophy. Section one explains how I integrate Cavendish into the narrative of my course, identifying both historical and thematic strategies for including her in introductory philosophy courses. Section two describes methods for teaching Cavendish’s natural philosophy and identifies a pedagogical challenge to presenting her criticisms of experimental philosophy. In their first exposure to Cavendish, …

An Argumental Discourse Before All: An Approach To Teaching Cavendish, Jonathan L. Shaheen

ABO: Interactive Journal for Women in the Arts, 1640–1830

Cavendish directs readers of Observations upon Experimental Philosophy to begin engaging her work through "An Argumental Discourse." In this piece, I describe a way of thinking about the structure of that piece and how to understand two of its major moments in relationship to standardly taught material. The first moment is the dilemma about how inanimate matter is moved, with its response centered on treating a horse and its rider as a paradigm example of causation. The article shows how the argument involves Cavendish's conceptions of causation, minds, bodies, and mind-body interaction. The second moment has to do with the …

Jumping Pens And Historical Vertigo: Teaching Cavendish In The Twenty-First Century, Anne M. Thell

ABO: Interactive Journal for Women in the Arts, 1640–1830

"Jumping Pens" considers "historical and epistemological vertigo" as a useful pedagogical rubric for teaching the works of Margaret Cavendish (1623-73) in contemporary university classrooms. Specifically, it looks back across more than a decade of teaching Cavendish in diverse settings in order to show how exciting this author can be for students who are just starting out in the discipline of literary studies. As this essay argues, Cavendish can make us all better, more self-aware instructors, while also developing critical and historical acumen in our students.

Concise Collections: Teaching Margaret Cavendish, Part Ii, E Mariah Spencer, Tiffany Potter

ABO: Interactive Journal for Women in the Arts, 1640–1830

The Winter 2024 edition of ABO continues the Concise Collections on Teaching series, centering on Margaret Cavendish, Duchess of Newcastle (1623–1673), an "also/and" author whose expansive work defies singular categorization. Cavendish’s prolific corpus engages with a wide array of subjects, from materialism and scientific anti-realism to gender, education, and cognition. Her writings bridge both literary and philosophical traditions, blending speculative fiction with critiques of mechanistic science, particularly through her explorations of weird fiction and proto-scientific thought.

Photo-Feminists: The Inter-Textual Discussion Of Eighteenth-Century Periodical Frontispieces, Karenza Sutton-Bennett

ABO: Interactive Journal for Women in the Arts, 1640–1830

The visual afterlife of the two frontispieces from the first volume of Eliza Haywood’s periodical, The Female Spectator (1744-46) promotes the argument that middling women should be allowed to learn the same proto-disciplinary topics that men studied by representing how women could include both amusements and informal learning in between their duties as wives and mothers. The Female Spectator’s frontispiece, the emblem to Frances Brooke’s The Old Maid (1755-56), the frontispiece to Charlotte Lennox’s The Lady’s Museum (1760-61) and the 1789 frontispiece to The Lady’s Magazine (1770-1847) have the common element of the image of Minerva to visually continue the …

Playing Proverbs With Madame De Genlis, Rebecca Cypess

ABO: Interactive Journal for Women in the Arts, 1640–1830

The theatrical genre known as the proverbe dramatique, which flourished as a pastime of French high society during the late eighteenth century, has often been understood through the written exemplars left by the author and artist Louis Carrogis, known as Carmontelle. Yet Carmontelle’s literary contributions to the genre have obscured the vast range of activities known as jouer des proverbes (playing proverbs)—activities associated especially with women—which included conversational games, pantomime, poetry, dance, and music making. Attending to the many descriptions of jouer des proverbes left by Stéphanie Félicité Ducrest de Saint-Aubin, the Com­tesse de Genlis, reveals that the literary …

The Sounds Of Madame Geoffrin’S Salon, Melanie Conroy

ABO: Interactive Journal for Women in the Arts, 1640–1830

Salons were core institutions of the eighteenth-century European social calendar, especially in Paris. Literary performances and philosophical conversation were the core activities of many of these gatherings. What did conversation and literary readings actually sound like at these events? To what extent were voices audible to everyone in the room, or too muffled or far away to be heard? What was the impact of the ambiance or of ephemeral sound on the reading or reception of the hearing? Using textual descriptions, paintings, and architectural information, I reconstruct two legendary readings of eighteenth-century texts, Voltaire’s L’Orphélin de la Chine and Marmontel's …

Enfilade And Variations: Interpreting Salon Music Through Residential Architecture In Eighteenth-Century Paris, Nicole Vilkner

ABO: Interactive Journal for Women in the Arts, 1640–1830

In eighteenth-century Parisian salons, theme and variations on popular melodies proliferated. Elaine Sisman’s seminal study of variation form provides a foundational approach for understanding variations as rhetorical exercises that persuade through differences in syntax and expression. In this vein, scholars have comprehensively affirmed the ways that salon music reflects the dialogic and sociable practices of Enlightenment receptions (Hanning, Sutcliffe, and Zohn). I suggest that theme and variation form is not only connected to the rhetoric of the salon, but that the concept of sequential elaboration is a principle that was also prized in residential architecture.

Drawing from theoretical writings of …

Simulating An Eighteenth-Century Enlightenment Salon: Embodied Experience And Scholarly Investigation, Jennifer M. Jones

ABO: Interactive Journal for Women in the Arts, 1640–1830

This paper reflects on my experience simulating an 18th-century French salon for an academic conference and in an undergraduate classroom. I argue that simulating a salon is heuristically as well as pedagogically useful and allows historians to test hypotheses about the nature of the salon and the role of the salonnière proposed by scholars such as Dena Goodman and Antoine Lilti. The essay also engages with some of the important insights gained from feminist and performance theory regarding historical reenactment. Thus I caution against naive attempts to reenact the past and instead suggest practices that foster open-ended conversations about the …

Toxic Varnishes: Perfumes, Stench, And Glow In Eighteenth-Century French Salons, Érika Wicky

ABO: Interactive Journal for Women in the Arts, 1640–1830

As several historians, including Alain Corbin, have noted, a new sensitivity to odors emerged in the 18^th century that increased people’s negative responses to strong smells and heightened the demand for fragrances that were not only pleasant but also light and subtle. Some scholars see this period as a true “olfactory revolution.” How was this expressed in the salon, perceived both as a luxurious space and a site of elite sociability? This focus on the smell of varnish aims to illustrate how the scent of materials intertwined with the pleasures of decorative arts, both in the entire space of …

Introduction: The Salon And The Senses In Eighteenth-Century France, Jennifer M. Jones, Rebecca Cypess

ABO: Interactive Journal for Women in the Arts, 1640–1830

An introduction to a special collection of essays exploring the salon and the senses in eighteenth-century France. Provides an overview of the critical historiography on the salon and the role of salon hostesses. Explores new approaches to salon within the context of the history of the senses, with attention to the role of art, music, and sociability in salon culture. Provides an overview of the essays in the collection.

Leading Towards The Queerest Insurrection: Queer Anarchism And Leadership Studies, Josie Holland

Interdisciplinary Journal of Leadership Studies

Leadership shapes our world, our narratives, and what is possible to imagine. Unfortunately, leadership studies has some trouble imagining queer leadership, or rather, queer theory and leadership studies. In order to integrate the more abstract aspects of queer theory with the largely evidence-based field of leadership studies, I draw on the texts, history, practices, and theory of queer anarchism. This article aims to highlight a gap in leadership studies as it currently stands, and through accounting for the absence of queer anarchism in leadership studies, expand what leadership looks like. To this end, I suggest the framing of ‘leadership acts’ …

From Surviving To Thriving: Key Considerations For Weight Control Across Diverse Cancer Survivorship Populations, Justin C. Brown, Marlyn Allicock, Carmina G. Valle, Tanya Agurs-Collins

School of Medicine Faculty Publications

BACKGROUND: Obesity is a chronic, relapsing, progressive disease of excess adiposity that increases the risk of dying from at least 16 types of cancer. The prevalence of obesity has increased more rapidly in cancer survivors compared with the general population. Tailored weight management strategies are needed to improve prognosis and health outcomes in the growing population of cancer survivors. However, certain cancer survivor population subgroups require unique consideration when developing weight management strategies. METHODS: In a symposium convened by The Obesity Society during ObesityWeek 2023 titled "From Surviving to Thriving: Key Considerations for Weight Control Across Diverse Cancer Survivorship Populations," …

A Cross-Cultural Comparative Study Of Electric Vehicle Advertisements From The Perspective Of Visual Grammar, Hu Yumeng, Yizhou Yuan

Visions in Leisure and Business

This article aims to explore the application of visual grammar in a cross-cultural context. Taking the visual grammar performance of electric car advertisements in different cultural backgrounds as an example, it analyzes how visual elements in these advertisements reflect visual grammar from a cross-cultural perspective, focusing on the principles of cross-cultural business communication. Based on the visual grammar theory of Kress and van Leeuwen, this study selected electric vehicle advertising samples from China and the United States and analyzed them using content analysis. The study found that although global electric vehicle advertisements have commonalities in conveying core values such as …